阅读说明：本技术 一种使用正丁醇提取银杏黄酮的方法 (Method for extracting ginkgetin by using n-butanol ) 是由 原龙 王晓军 刘伶文 刘茜倩 胡世义 于 2019-10-21 设计创作，主要内容包括：本发明公开一种使用正丁醇萃取银杏黄酮的方法,涉及植物功能成分提取技术领域,具体为一种从银杏叶中提取银杏黄酮的方法。本方法以乙醇为溶剂,用超声辅助浸提法从银杏叶粉末中得到银杏叶粗提液；以正丁醇为溶剂对所得的银杏叶粗提液进行纯化分离,干燥得到银杏黄酮。本发明具有操作简便、对设备要求低、以正丁醇为溶剂成本低、萃取率高等特点,可为正丁醇为溶剂萃取银杏黄酮的工业化生产提供借鉴。(The invention discloses a method for extracting ginkgetin by using n-butanol, relates to the technical field of plant functional component extraction, and particularly relates to a method for extracting ginkgetin from ginkgo leaves. The method takes ethanol as a solvent, and obtains a ginkgo leaf crude extract from ginkgo leaf powder by an ultrasonic-assisted extraction method; and (3) purifying and separating the obtained ginkgo leaf crude extract by using n-butanol as a solvent, and drying to obtain the ginkgo flavone. The method has the characteristics of simple and convenient operation, low requirement on equipment, low cost by taking the n-butyl alcohol as the solvent, high extraction rate and the like, and can provide reference for the industrial production of extracting the ginkgetin by taking the n-butyl alcohol as the solvent.)

1. A method for extracting ginkgetin by using n-butanol is characterized by comprising the following steps:

step 1, preparing ginkgo leaf powder from ginkgo leaves, extracting the ginkgo leaf powder with ethanol under the assistance of ultrasonic waves to obtain ginkgo leaf crude extract, and performing rotary evaporation on the obtained ginkgo leaf crude extract to obtain ginkgo leaf extract.

And 2, diluting the ginkgo leaf extract by using distilled water to obtain a diluted extract, extracting the diluted extract by using n-butyl alcohol, separating liquid to obtain a crude extract, performing rotary evaporation on the crude extract to obtain an extract, and drying and concentrating the extract to obtain the ginkgo flavone.

2. The method for extracting ginkgetin using n-butanol according to claim 1, wherein step 1 is specifically performed according to the following steps:

step 1.1, drying clean and fresh ginkgo leaves, crushing the dried and fresh ginkgo leaves into powder, and sieving the powder by a 200-mesh sieve to obtain ginkgo leaf powder;

step 1.2, mixing the ginkgo leaf powder obtained in the step 1.1 with ethanol, and performing ultrasonic extraction after mixing to obtain a ginkgo leaf crude extract;

and step 1.3, placing the ginkgo leaf crude extract obtained in the step 1.2 in a rotary evaporator, and performing rotary evaporation to obtain the ginkgo leaf extract.

3. The method for extracting ginkgetin using n-butanol according to claim 2, wherein the drying temperature of ginkgo leaves in step 1.1 is 70-90 ℃.

4. The method for extracting ginkgo biloba leaves through n-butanol according to claim 2, wherein the mixing ratio of the ginkgo biloba leaves powder to the ethanol in the step 1.2 is 1 g/6-9 mL, and the ethanol is 70-90% by volume.

5. The method for extracting ginkgetin by using n-butanol according to claim 2, wherein the parameters of ultrasonic extraction include ultrasonic power of 180-200W, ultrasonic action at intervals of 0.5s for 5-7 s, and ultrasonic extraction time of 30-50 min.

6. The method for extracting ginkgetin using n-butanol according to claim 2, wherein the 1.3 rotary evaporation parameters include pressure of 0.08-0.09 MPa, rotation speed of 70-90 r/min, and temperature of 50-70 ℃.

7. The method for extracting ginkgetin using n-butanol according to claim 2, wherein said step 2 is carried out by the following steps:

step 2.1, sucking 10-20mL of ginkgo leaf extract, adding 10mL of distilled water for dilution to obtain diluted extract, mixing the diluted extract with n-butyl alcohol, extracting, pouring into a separating funnel for standing, separating liquid after solution layering to obtain crude extract, placing the crude extract in a rotary evaporator, and performing rotary evaporation to obtain extract;

and 2.2, drying and concentrating the extract liquor to obtain the ginkgetin.

8. The method for extracting ginkgetin using n-butanol according to claim 7, wherein the diluted extract is mixed with n-butanol at a ratio of 1 g: 2-5mL, wherein the volume fraction of the n-butanol is 80-90%.

9. The method for extracting ginkgetin using n-butanol according to claim 8, wherein the diluted extract is mixed with n-butanol in step 2.1, and then extracted at 60-65 ℃ for 8-10 min.

Technical Field

The invention belongs to the technical field of plant functional component extraction, and relates to a method for extracting ginkgetin by using n-butanol.

Background

As a kind of common natural medicines, ginkgetin has different extraction and purification methods, and the methods recorded according to the literature currently include: solvent method, ultrasonic extraction method, microwave extraction method, capillary electrophoresis method, macroporous adsorption resin method, etc. The novel separation technology is adopted, and the breakthrough and innovation of the current separation technology are imperative in order to further purify the functional substances in the ginkgo, give full play to the advantages of ginkgo resources to a greater extent and promote the deep research on the aspects of the structure, pharmacology, drug effect and the like of plant effective substances. The optimization of the processing technology of the ginkgo leaves, the innovation of the extraction technology and the establishment of the high-efficiency and quick separation and purification identification technology of trace effective substances in the ginkgo leaves can improve the utilization rate of the ginkgo leaves and medicinal compounds thereof. The technology for realizing rapid separation and purification is essential to generating great economic benefit, medicinal value, protecting environment and the like. The ultrasonic extraction method is expensive in equipment and requires high heat resistance for the treated substance; the macroporous adsorption resin has the defects of high cost, large operation scale and the like, and the ginkgo flavone is extracted and purified by adopting an organic solvent, namely n-butyl alcohol, because the organic solvent extraction method is the most commonly used method at the present stage, but acetone is commonly used as an extracting agent at home and abroad, and the subsequent treatment is more complicated.

Disclosure of Invention

The invention aims to provide a method for extracting ginkgetin by using n-butanol, which solves the problem that the subsequent treatment of the common extractant acetone extraction operation in the prior art is more complicated.

The technical scheme adopted by the invention is that the method for extracting the ginkgetin by using the n-butanol is implemented according to the following steps:

step 1, preparing ginkgo leaf powder from ginkgo leaves, extracting the ginkgo leaf powder with ethanol under the assistance of ultrasonic waves to obtain ginkgo leaf crude extract, and performing rotary evaporation on the obtained ginkgo leaf crude extract to obtain ginkgo leaf extract.

And 2, diluting the ginkgo leaf extract by using distilled water to obtain a diluted extract, extracting the diluted extract by using n-butyl alcohol, separating liquid to obtain a crude extract, performing rotary evaporation on the crude extract to obtain an extract, and drying and concentrating the extract to obtain the ginkgo flavone.

The invention is also characterized in that:

the step 1 is implemented according to the following steps:

step 1.1, drying clean and fresh ginkgo leaves, crushing the dried and fresh ginkgo leaves into powder, and sieving the powder by a 200-mesh sieve to obtain ginkgo leaf powder;

step 1.2, mixing the ginkgo leaf powder obtained in the step 1.1 with ethanol, and performing ultrasonic extraction after mixing to obtain a ginkgo leaf crude extract;

and step 1.3, placing the ginkgo leaf crude extract obtained in the step 1.2 in a rotary evaporator, and performing rotary evaporation to obtain the ginkgo leaf extract.

In the step 1.1, the drying temperature of the ginkgo leaves is 70-90 ℃.

In the step 1.2, the mixing ratio of the ginkgo leaf powder to the ethanol is 1g to 6-9mL, and the volume fraction of the ethanol is 70-90%.

The parameters of ultrasonic extraction comprise ultrasonic power of 180-200W, ultrasonic action is performed every 5-7 s at intervals of 0.5s, and ultrasonic extraction is performed for 30-50 min.

The 1.3 kinds of rotary evaporation parameters comprise pressure of 0.08-0.09 MPa, rotating speed of 70-90 r/min and temperature of 50-70 DEG C

The step 2 is implemented according to the following steps:

step 2.1, sucking 10-20mL of ginkgo leaf extract, adding 10mL of distilled water for dilution to obtain diluted extract, mixing the diluted extract with n-butyl alcohol, extracting, pouring into a separating funnel for standing, separating liquid after solution layering to obtain crude extract, placing the crude extract in a rotary evaporator, and performing rotary evaporation to obtain extract;

and 2.2, drying and concentrating the extract liquor to obtain the ginkgetin.

In the step 2.1, the mixing ratio of the diluted extracting solution to the n-butyl alcohol is 1 g: 2-5mL, and the volume fraction of the n-butyl alcohol is 80-90%.

In step 2.1, mixing the diluted extract with n-butanol, and extracting at 60-65 deg.C for 8-10 min.

The invention has the beneficial effects that: the method adopts the organic solvent n-butyl alcohol to extract and purify the ginkgetin, and has the characteristics of simple and convenient operation, low requirement on equipment, low cost by taking the n-butyl alcohol as the solvent, high extraction rate and the like. The invention can provide reference for the industrial production of extracting the ginkgetin by using the n-butyl alcohol as a solvent.

Detailed Description

The present invention will be described in detail with reference to the following embodiments.