阅读说明：本技术 用于在数字微流控装置中过滤小体积非均相悬浮液的方法 (Method for filtering small size heterogeneous suspension in digital microcurrent-controlled device ) 是由 C·A·狄克逊 J·L·拉曼纳 A·R·惠勒 于 2018-04-03 设计创作，主要内容包括：本文中公开了用于液体的数字微流控(DMF)处理的两种相关技术,所述技术依赖于对流体静电致动使之通过固体多孔介质的条带。在第一种技术中,驱动DMF装置中的含有不同大小的颗粒的小滴通过固体多孔介质,允许基于大小来过滤、浓缩所述颗粒并将所述颗粒回收到小滴中。在第二种技术中,将液体介质的试样直接装载到固体多孔介质上,其中所述液体介质渗入到DMF装置中,使得滤液能够被收集到小滴中。两种技术都可用于在DMF装置上由全血产生血浆,这是对数字微流控的诊断应用具有深远影响的操作。(There is disclosed herein two kinds of the relevant technologies of digital microcurrent-controlled (DMF) processing for liquid, the technology depends on the band that solid porous media is run through to fluid electrostatically actuated.In the first technology, drives the droplet containing different size of particle in DMF device by solid porous media, allow to filter based on size, the particle is concentrated and the particle is recovered in droplet.In second of technology, the sample of liquid medium is loaded directly on solid porous media, wherein the liquid medium penetrates into DMF device, filtrate is collected into droplet.Two kinds of technologies can be used in generating blood plasma by whole blood on DMF device, this is the operation for having profound influence to digital microcurrent-controlled diagnostic application.)

1. it is a kind of for filter heterogeneous suspension based on digital microcurrent-controlled method, which comprises

A) porous material is positioned at the bottom plate in digital microcurrent-controlled device and between top plate, the porous material has pre-selection hole Porosity is to prevent the particle for being greater than pre-selection size from passing through, while the particle for allowing to be less than the pre-selection size passes therethrough；

B) droplet containing different size of particle is applied on the electrod-array being fitted on the bottom plate and with certain side Formula activates the electrod-array to drive the droplet from the first side of the porous material to second side by the porous material Material so that the particle for being less than the pre-selection size passes through the porous material to form filtered droplet, and is greater than institute The particle for stating pre-selection size is trapped in the porous material or is trapped within the porous material in first side Surface on；

C) electrod-array is activated so that the filtered droplet moves away from the porous material to wait further place Reason；

D) droplet that buffer is resuspended is applied in described second side of the porous material and is fitted on the electrod-array and causes The electrod-array is moved to drive the droplet that buffer is resuspended from described second side of the porous material to described first Side is by the porous material, so that the biggish particle that is trapped is removed from the porous material and in the porous material First lateral suspension to it is described be resuspended buffer droplet in；And

E) electrod-array is activated so that the droplet that buffer is resuspended moves away from the porous material with optionally etc. Wait be further processed.

2. according to the method described in claim 1, wherein the porous material is the porous material with length and opposing end portions Band, and wherein the opposing end portions include being embedded in hydrophobic material therein to prevent liquid from leaving the opposing end portions.

3. method according to claim 1 or 2, wherein the digital microcurrent-controlled device is configured for veneer operation.

4. method according to claim 1 or 2, wherein the digital microcurrent-controlled device is configured for double plate operations.

5. method according to claim 1,2,3 or 4, wherein the porous material is two or more porous material items Band, the porous material band are sequentially disposed in the digital microcurrent-controlled device, so that the droplet is by described two Or more porous material band, wherein the porous material band be chosen to have the hole size successively decreased with filter have it is more A different size of droplet.

6. according to claim 1, method described in 2,3 or 5, wherein the droplet is the droplet of human body fluid or animal body fluid.

7. according to the method described in claim 6, wherein the droplet is the droplet of people's blood, Seminal plasma and urine.

8. according to the method described in claim 6, wherein the droplet is the droplet of people's blood, and wherein the method is configured For generating blood plasma by whole blood.

9. it is a kind of for filter heterogeneous suspension based on digital microcurrent-controlled method, which comprises

A) porous material is positioned at the bottom plate in digital microcurrent-controlled device and between top plate, the porous material has pre-selection hole Porosity is to prevent the particle for being greater than pre-selection size from passing through, while the particle for allowing to be less than the pre-selection size passes therethrough, described Porous material has the loading station at the edge for extending beyond the top plate；

B) the sample droplet containing different size of particle is applied on the loading station for being fitted on the porous material, so that institute Sample droplet is stated to penetrate into the porous material；

C) reagent is applied on the electrod-array being fitted on the bottom plate and activate in some way the electrod-array with Drive the droplet from the first side of the porous material to second side by the porous material, so that it is big to be less than the pre-selection The small particle flow through the porous material with obtain having in described second side the smaller particle containing particle Reagent droplet, and the particle for being greater than the pre-selection size remains trapped in the porous material；And

C) activate the electrod-array so that the reagent droplet containing particle move away from the porous material wait into The processing of one step.

10. according to the method described in claim 9, the method includes separation membranes can be laminated to the institute with pre-selection porosity Loading station is stated, the sample reagent is loaded on the loading station.

11. method according to claim 9 or 10, wherein the porous material is have length and opposing end portions porous Material bands, and wherein the end opposite with the loading station of multi-well strip include be embedded in hydrophobic material therein with Prevent liquid from leaving the end of the band.

12. according to method described in claim 9,10 or 11, wherein the digital microcurrent-controlled device is configured for veneer behaviour Make.

13. according to method described in claim 9,10 or 11, wherein the digital microcurrent-controlled device is configured for double plate behaviour Make.

14. the method according to any one of claim 9 to 13, wherein the droplet is the small of human body fluid or animal body fluid Drop.

15. according to the method for claim 14, wherein the droplet is the droplet of people's blood, Seminal plasma and urine.

16. according to the method for claim 15, wherein the droplet is the droplet of people's blood, and wherein the method is matched It sets for generating blood plasma by whole blood.

Technical field

This disclosure relates in digital microcurrent-controlled device filter small size heterogeneous suspension based on digital miniflow Control the method and system of (DMF).

Background technique

Although be macroscopically it is very universal, homogeneous mixture is filtered in micro fluidic device and/or Separation has become the object studied recently.In micro-fluidic field, several filtering policies have been described, that is, active technique, such as Dielectrophoresis (DEP), magnetic and method for acoustic (bibliography 1) and passive technology, including certainty lateral displacement, cross-flow filtration, Hydrodynamic force filtering and folder flow through filter (bibliography 2).For former (active) technology, external control place needed for applying needs Equipment increase complexity and cost to micro fluidic device, and latter (passive) technology usually require pump and valve system and Continuous flow filters and separates particle.Influencing another typical challenge based on micro-fluidic filtration system is to form the dress It sets required manufacturing method and is usually directed to and complicated based on the technology of toilet and need individual design iteration and form energy Enough filter the structure of the particle of different size or grade.

Digital microcurrent-controlled (DMF) is steady liquid handling technology, and the technology includes using electrostatic force to discrete skin The liquid droplets for being raised to microlitre size are manipulated (bibliography 3).DMF and the micro-fluidic difference of traditional continuous flow, that is, be not Continuous flow is in channel, but droplet is clipped between electrode top plate and the bottom plate for the array for carrying insulation driving electrodes.In In this configuration, it can apply and match, mix, merge and separate individual droplet, each droplet serves as different microreactors, makes DMF becomes powerful sample disposition and chemical treatment method.Importantly, DMF be it is reconfigurable-can be used one As device architecture and the general device architecture be recycled and reused for virtually limitless operative combination.

So far, also few examples are filtered and/or separated in DMF device.A kind of method is related in described device Porous polymer material all in one piece (PPM) disk (bibliography 4) for solid phase extractions of interior formation, and other methods use antibody function Magnetic bead can be changed to separate biomolecule from complex matrices (such as blood or serum) and (bibliography 5) is isolated.Although success , but the manufacturing step (manufacture of PPM disk) that these methods needs are complicated and time-consuming, especially functionalization magnetic bead (and The magnet being mounted on stepping motor), or for will the diluted sample outside piece before analysis.Finally, developing use Gravity and hydrodynamic force separate the DMF method (bibliography 6) of particle in the device.However, the range of this method is limited, because It requires non-floating particle and the particle those of in certain size range.

Summary of the invention

There is disclosed herein two kinds of the relevant technologies for DMF, the technology depends on and is allowed to logical to fluid electrostatically actuated Cross solid porous media band.In technology (1), the droplet containing different size of particle in DMF device is driven to pass through solid Body porous media is allowed to be filtered based on size, the particle is concentrated and the particle is recovered in droplet.In technology (2) In, the sample of liquid medium is loaded directly on solid porous media, wherein the sample of the liquid medium penetrates into DMF dress In setting, filtrate is collected into droplet.Two kinds of technologies can be used in generating blood plasma by whole blood on DMF device, this It is the operation that there is profound influence to digital microcurrent-controlled diagnostic application.

In the first embodiment, provide it is a kind of for filter heterogeneous suspension based on digital microcurrent-controlled method, The described method includes:

A) porous material is positioned at the bottom plate in digital microcurrent-controlled device and between top plate, the porous material has pre- Porosity is selected to prevent the particle for being greater than pre-selection size from passing through, while the particle for allowing to be less than the pre-selection size passes therethrough；

B) droplet containing different size of particle is applied on the electrod-array being fitted on the bottom plate and with certain Kind mode activates the electrod-array to drive the droplet from the first side of the porous material to second side by described more Porous materials so that the particle for being less than the pre-selection size passes through the porous material, and are greater than the institute of the pre-selection size It states particle and is trapped in the porous material or is trapped within the porous material on described first surface；

C) electrod-array is activated so that filtered droplet moves away from the porous material to wait further place Reason；

D) droplet that buffer is resuspended is applied in described second side of the porous material and is fitted on the electrod-array simultaneously And the actuating electrod-array is to drive the droplet that buffer is resuspended from described second side to first side by described Porous material, so that the biggish particle that is trapped removes from the porous material and is suspended to the small of the resuspension buffer In drop；And

E) electrod-array is activated so that the droplet that buffer is resuspended moves away from the porous material to wait into one Step processing.

In this second embodiment, provide it is a kind of for filter heterogeneous suspension based on digital microcurrent-controlled method, The described method includes: a) porous material is positioned at the bottom plate in digital microcurrent-controlled device and between top plate, the porous material With pre-selection porosity to prevent the particle for being greater than pre-selection size from passing through, while allowing to be less than the particle of the pre-selection size therefrom Pass through, the porous material has the loading station at the edge for extending beyond the top plate；

B) the sample droplet containing different size of particle is applied on the loading station for being fitted on the porous material, is made The sample droplet is obtained to penetrate into the porous material；

C) reagent is applied on the electrod-array being fitted on the bottom plate and activates the electrode array in some way Column are to drive the droplet from the first side of the porous material to second side by the porous material, so that being less than described pre- Select the particle of size to flow through the porous material with obtain having in described second side the smaller particle containing The reagent droplet of grain, and the particle for being greater than the pre-selection size remains trapped in the porous material；And

C) electrod-array is activated so that the reagent droplet containing particle moves away from the porous material comes Wait be further processed.

By reference to described in detail below and schema, may be implemented in terms of the function of the disclosure and advantageous aspect into one Step understands.

Detailed description of the invention

It only illustrates referring now to schema to describe embodiment, in the drawings:

Figure 1A shows a kind of DMF device, and the DMF device is modified and including for separating the different size in fluid Particle perforated membrane；

Figure 1B (i) to Figure 1B (iv), which is shown, implements different step in the device separation process disclosed herein of Figure 1A Sequence；

Fig. 1 C shows the photograph (In for being painted the result of the particle separating experiment carried out using the DMF device of Figure 1A and Figure 1B UV irradiation is lower to collect)；

Fig. 2A shows another embodiment of DMF device, the DMF device it is modified and including for separating fluid In different size of particle two perforated membranes；

Fig. 2 B (i) to Fig. 2 B (iii), which is shown in the device separation process disclosed herein of Fig. 2A, implements different step Sequence；

Fig. 2 C (i) to Fig. 2 C (ii) shows the photograph for being painted the sequence of the separating experiment carried out using the DMF device of Fig. 2A；

Fig. 2 D shows the representative result of the whole blood sample handled as shown in the sequence of Fig. 2 B；

It is opposite compared with untreated whole blood that Fig. 2 E shows the whole blood sample handled as shown in the sequence of Fig. 2 B Purity (counting of red blood cell)；

Fig. 3 shows another embodiment of DMF device, the DMF device it is modified and including with the DMF of Figure 1A dress It sets compared to positioned at the perforated membrane for being used to separate the different size of particle in fluid being differently directed；And

Fig. 4 shows another embodiment of DMF device, the DMF device it is modified and including for separating fluid In a variety of different size of particles multiple consecutive trackings perforated membrane.

Specific embodiment

The various embodiments and aspect of the disclosure will be described with reference to the details being discussed herein below.Be described below is with schema For illustrating the disclosure and being understood not to the limitation disclosure.Schema is not drawn on scale.Describe numerous details with Thorough understanding to the various embodiments of the disclosure is provided.However, in some cases, not describing known or conventional details It is discussed in order to provide the simplicity of the embodiment to the disclosure.

As used herein, term " includes " and " including " will be understood to is that inclusive and opening, and non-exclusive Property.Specifically, ought be in the specification and in the claims in use, term " includes " and " including " and its variant table Show including specified feature, step or component.These terms will not be interpreted to exclude depositing for other feature, step or component In.

As used herein, it term " exemplary " expression " be used as example, example or illustration " and will not be understood It is preferably or more advantageous than other configurations disclosed herein.

As used herein, term " about " and " about " intend the upper limit and lower limit change that may be present that cover value range Change, such as variation of property, parameter and size.In one non-limiting example, term " about " and " about " indicate to add deduct Percent 10 or less.

Unless otherwise defined, otherwise all scientific and technical terminologies used herein are intended to the ordinary skill people with this field The identical meaning of meaning that member is generally understood.

As used herein, in the background of the disclosure, term " particle " indicates can be with body phase fluid in fluid media (medium) (including cell) distinguish anything.

In technology (1), by applying voltage to series of electrodes to drive hold-up of the droplet by porous material Item bring and filter out particle from droplet, each end of the band is anchored by hydrophobic barrier and is clipped in DMF device Between top plate and bottom plate.The particle that diameter is greater than the aperture of porous media is trapped within item and takes, and liquid (including diameter is less than The suspension of the particle in the aperture of porous media) pass therethrough the arrival other side.It then can be by making fresh droplet in phase The item is passed back through on opposite direction brings collection to be trapped particle on the filter.Pass through by adjusting the movement of droplet one way The number (and volume) of porous media, can be concentrated or dilute particle.

Figure 1A shows DMF device 10, and the DMF device is modified and including different size of in fluid for separating The porous material 12 of particle.Fluid and porous material 12 are clipped in the DMF bottom plate 16 and top plate pair for carrying the array of driving electrodes Between electrode 18.Porous material 12 can be any medium with the hole less than particle to be separated.Porous material 12 includes Positioned at the hydrophobic anchor 20 of the end of the material to prevent liquid to be exuded to around the end of material 12.Porous material 12 it is non- Limitative examples include based on polymer, based on fiber or some combined perforated membranes of the rwo.Figure 1A, which is shown, to be determined Droplet 24 of the position to be filtered.

The exemplary illustration of technology (1) is shown in fig. ib, and preliminary data is shown in Figure 1C.In latter feelings In condition, being handled using technology (1) on DMF device 10 containing diameter is respectively 0.3 μm and 10 μm of red and green fluorescence The droplet of the mixture of pearl.The band of porous material 12 has aperture, so that 10 μm of particles of green are trapped, and the droplet Pass through (28) with lesser red granules.Green particles are then collected into the fresh droplet of buffer (34).

More particularly, Figure 1A, Figure 1B and Fig. 1 C illustrate technology (1), and wherein Figure 1A to Figure 1B (iv) illustrates that DMF is filled The step of droplet filtering set and particle are resuspended.In figure 1A, as discussed above, by hydrophobic barrier 20 by porous material The both ends of 12 band anchor to DMF device 10 top plate or bottom plate (in this case, via paraffin) and by assembling So that medium 12 is between corresponding top plate 18 and bottom plate 16.It has applied with droplet 24 and the droplet was ready for Filter, shows in the left-hand side of porous material 12.It applies with droplet 24 to be filtered and a company is applied by the electrode to bottom plate 16 Voltage go here and there to activate the droplet.In Figure 1B (i), when droplet passes through porous material 12, the particle of material 12 can not be passed through The item is trapped within to take, and the filtrate for being less than any smaller particle in the aperture of porous material 12 containing size proceeds to separately Side.In Figure 1B (ii), particle is trapped within as shown in the figure in porous material 12, and filtered droplet 28 moves away from Porous material 12 is shown in the right-hand side of multi-well strip 12.In Figure 1B (iii), in order to collect the particle being trapped, Shi Peichong It hangs the droplet 32 of buffer and is activated the other way around towards multi-well strip material 12 (right-hand side of band 12 to left and right side) The droplet.In Figure 1B (iv), when buffer passes through the band, the particle being trapped leaves band 12 and is suspended to In droplet 34.

Fig. 1 C shows the photograph (collecting under uv illumination) for the result for being painted particle separating experiment.Firstly, making containing 0.3 μm The droplet of the mixture of the particle of the particle of diameter and biggish 10 μ m diameter from left to right passes through the band of porous material 12, institute The both ends for stating band are anchored by hydrophobic barrier 20.It can be seen in the right-hand side of photograph resulting through filtering droplet 28, the warp Filtering droplet contains the particle of 0.3 μ m diameter.Second, it applies the droplet with buffer and the droplet is made from left to right to pass through institute Band is stated, releases the larger particles of retention in the porous material in this way, and gained droplet can be seen in the left-hand side of photograph 34。

Band of the technology (2) as technology (1) dependent on the porosu solid medium in DMF device.But in technology (2) In, liquid suspension to be filtered is loaded directly into porous media to (porous media can be " edge " of band Either it is laminated to individual " film " at the edge of band).When solution penetrates through mounting medium, diameter is greater than fixed material Aperture particle be trapped on the top of the medium or within, and fluid flows in band via capillary force.Then Make the droplet of buffer by band to collect solute or little particle.

Fig. 2A shows another embodiment of DMF device 50, and the DMF device is modified and flows including being used to separate Two perforated membranes 52 and 54 of the different size of particle in body.It in this embodiment, will be with the porous of hydrophobic anchor 64 Film 54 is placed between the top plate 56 of DMF device 50 and bottom plate 58, and wherein one end extends beyond the edge of top plate 56.By second point On top from the first perforated membrane 54 of the outside that film 52 is laminated to top plate 56.Perforated membrane 54 is used as by separated fluid The medium being transported in DMF device 50 later recycles the fluid in the DMF device.To be separated is contained The droplet 68 of particle suspension liquid is fed in seperation film 52 as shown in Figure 2 A.Source positioning to buffer 62 is resuspended, so that When needing the buffer, droplet 72 can be moved to 54 side of porous material, such as in fig. 2 in the left hand of material 54 Shown in side.The purpose of seperation film 52 is that the particle suspension liquid 68 for allowing to be filtered directly applies without any prior pretreatment Onto described device, and all unwanted particles present in suspension 68 are captured, while allowing other desired components It is moved in multi-well strip 54 via capillarity.

The explanatory illustration of method (2) is shown in Fig. 2 B (i) to Fig. 2 B (iii), and in Fig. 2 C, Fig. 2 D and Fig. 2 E Preliminary data is shown.Fig. 2 C (i) shows a sheet photo, wherein 50 μ L samples of dye solution are loaded on DMF device 50, Make perforated membrane 52 and 54 full of dyestuff.By the fresh droplet 62 for the buffer that is unstained from left to right by collecting the more of dyeing liquid Pore membrane 54 (such as in Fig. 2 B (iii)).Fig. 2 C (ii) show the right-hand side in the photograph it is visible it is resulting extract it is small It drips (80), the droplet contains blue dyes.The region that blue dyes is run out of in perforated membrane 54 is also visible.

In Fig. 2 D and Fig. 2 E, 50 μ L samples of whole blood are loaded on DMF device 50, wherein cell and other particle quilts It is trapped in film 52.Blood plasma (such as in Fig. 2 B (iii)) is collected using the fresh droplet of buffer, is then surveyed using protein Kit is determined by measuring optical density at 620nm to assess the protein content of the blood plasma.For reference, further it is shown that use The protein content of gold standard blood plasma isolation technics (centrifugal process) recycling.Protein recovery is by respectively such as the sequence of Fig. 2 B Shown in as handle and Jing Guo gold standard technical treatment whole blood sample generation blood plasma droplet between protein concentration Ratio.

By calculate after using series processing whole blood sample shown in Fig. 2 B remaining red blood cell number come The purity of blood plasma droplet caused by measuring.For reference, further it is shown that the erythrocyte counts in whole blood.Separative efficiency is to be moved The percentage of the red blood cell removed.These data are preliminary；We believe firmly the volume by adjusting blood, film and porous material Size and recycle the number and volume of droplet, the new technology will reach on simple hand-held DMF device completely At with the comparable rate of recovery of gold standard technology, the DMF device is operated without any movable part.In addition, Primary evidence (being not shown herein) shows the hemolysis levels observed for the new technology and is directed to what gold standard was observed Hemolysis levels are suitable or lower.

More particularly, Fig. 2A, Fig. 2 B (i) illustrate technology (2) to Fig. 2 B (iii), Fig. 2 D and Fig. 2 E, and wherein Fig. 2A is extremely Fig. 2 B (iii) is illustrated the step of filtering on DMF device.In fig. 2, by hydrophobic barrier 64 by the item of porous material 54 One end of band anchors to the corresponding top plate 56 of DMF device 50 or bottom plate 58 (in this case, via paraffin) and passes through Assembling is so that medium is located between the top plate 56 and bottom plate 58 of DMF device 50.The geometry of band 54 is designed to make one End extends beyond the boundary of top plate 56.If desired, additional seperation film 52 can be laminated to the free end of the band.In In Fig. 2 B (i), the sample 68 of the suspension of solid is loaded on film/band 52, so that fluid penetrates into device 50, is made more Porous materials 54 are saturated.In Fig. 2 B (ii), pass through buffer droplet 72 by applying series of voltage to 58 electrode of bottom plate The band of porous material 54.In Fig. 2 B (iii), by the suspension of solute, (and/or diameter is less than the aperture of film/medium 54 The suspension of particle) it is collected into droplet 80.

Fig. 2 D shows the representative result of the whole blood sample handled as shown in Fig. 2 B (i) to Fig. 2 B (iii).Protein The rate of recovery indicates ratio of the amount of the protein in plasma sample compared with the blood plasma for using gold standard centrifugation technique to generate. " DMF-1 ", " DMF-2 ", " DMF-3 " and " DMF-4 " indicates that the blood plasma recycled on DMF device from individual blood sample is small Drop, and " centrifugation " is the blood plasma recycled by gold standard centrifugation technique.To " DMF-1 ", " DMF-2 ", " DMF-3 " and " DMF- 4 " protein recovery is corrected to take into account the volume of the blood plasma extracted from perforated membrane 54.

Fig. 2 E shows the data of the separative efficiency for the method summarized in Fig. 2 B (i) to 2B (iii).Briefly, 50 μ L samples of whole blood are loaded on DMF device 50, wherein cell and other particles are trapped in film 52.Use buffer Fresh droplet collect blood plasma (such as in Fig. 2 B (iii)), and calculated in recycled blood plasma droplet using hemocytometer Red blood cell (RBC) number (200RBC/ μ L).For reference, the RBC for showing whole blood counts (6.1x10⁶RBC/ μ L) and The RBC of the blood plasma recycled by centrifugation technique counts (0RBC/ μ L).Separative efficiency is by DMF or gold standard centrifugal method The percentage of the RBC of removal, and separative efficiency is also shown.

Fig. 3 shows another embodiment of DMF device 70, and the DMF device is modified and including the DMF with Figure 1A Device 10 compares the perforated membrane band 72 for being used to separate the different size of particle in fluid for being located at and being differently directed.Multi-well strip 72 have become longer, and compared with the orientation of the band 54 in the device of Figure 1A 10, the multi-well strip revolves in device 70 90 degree are turned, to allow to carry out multiple separation.

Fig. 4 shows another embodiment of DMF device 90, the DMF device 90 it is modified and including for separating The perforated membrane 92 of multiple consecutive trackings of a variety of different size of particles in fluid.Film 92 is placed in device 90, it is described Film is arranged based on the aperture successively decreased with sequentially filter can be in the particle recycled later.

It is discussed above it has been thought that DMF device is with the bottom plate of fixing DMF electrode, and top plate is to electrode.In this method Optional embodiment in, veneer DMF device can be used, wherein driving electrodes be coplanar to electrode.The veneer dress It sets with double panel assemblies the difference is that applying alive mode.Substitution applies driving voltage to bottom plate and applies to top Ground voltage applies driving voltage and ground voltage to the adjacent electrode on bottom plate.In this embodiment, program is kept As above situation.There is still a need for top plates, but top plate does not play the role of to electrode.

Being described above to the preferred embodiment of the disclosure is presented, to illustrate the principle of the disclosure but not incite somebody to action this It is open to be limited to illustrated specific embodiment.Wish the scope of the present disclosure by the appended claims and its equivalent intension All embodiments of lid limit.

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