阅读说明：本技术 用于逆转录病毒产生的稳定细胞系 (The stable cell lines generated for retrovirus ) 是由 C.文克 于 2018-04-18 设计创作，主要内容包括：本发明涉及逆转录病毒生产细胞,其包含编码以下的核酸序列：gag和pol蛋白；包膜蛋白或其功能性替代物；可扩增选择标记；和逆转录病毒载体颗粒的RNA基因组；其中所述核酸序列全部整合于逆转录病毒生产细胞基因组内的单一基因座处。本发明还涉及包含非哺乳动物复制起点且能够容纳至少25千碱基(kb)的DNA的核酸载体,其特征在于所述核酸载体包含编码以下的逆转录病毒核酸序列：gag和pol蛋白,和env蛋白或其功能性替代物。所述核酸载体另外包含编码可扩增选择标记的核酸序列。本发明还涉及使用所述核酸载体以产生稳定的逆转录病毒包装和生产细胞系的用途和方法。(The present invention relates to retroviral producer cells, and it includes encode nucleic acid sequence below: gag and pol albumen；Envelope protein or its functional replacement；Amplifiable selected marker；With the rna gene group of retroviral vector particle；Wherein the nucleic acid sequence is all integrated at the term single gene seat in retroviral producer cells genome.The invention further relates to comprising nonmammalian replication orgin and can accommodate at least 25 kilobase (kb) DNA nucleic acid carrier, it is characterized in that the nucleic acid carrier includes to encode retroviral nucleic acid sequence below: gag and pol albumen and env albumen or its functional replacement.The nucleic acid carrier additionally comprises the nucleic acid sequence for encoding amplifiable selected marker.The invention further relates to use the nucleic acid carrier to generate the purposes and method of stable retrovirus packaging and production cell line.)