阅读说明：本技术 一种北柴胡地上部分不同提取物的抗癫痫作用 (A kind of antiepileptic action of Bupleurum chinense DC difference extract ) 是由 杨炳友 刘艳 匡海学 于 2019-10-08 设计创作，主要内容包括：本发明涉及医药学领域,特别是涉及一种北柴胡地上部分不同提取物的抗癫痫作用的医药用途。通过水蒸气蒸馏法和溶剂回流提取法,制备了北柴胡地上部分的挥发油和乙醇提取物。药理学实验显示,北柴胡地上部分不同提取物均具有明显的抗癫痫作用,其中,乙醇提取物抗癫痫作用与北柴胡根相当、挥发油抗癫痫作用优于北柴胡根。北柴胡地上部分挥发油与拉莫三嗪联合使用的抗癫痫效果显著优于单用上述成分的效果,并且显著优于柴胡复方制剂与拉莫三嗪联合使用的抗癫痫效果,为中西药复方制剂的安全和质量可控提供了可能性。(The present invention relates to medicines, more particularly to a kind of medical usage of the antiepileptic action of Bupleurum chinense DC difference extract.By steam distillation and solvent refluxing extraction method, it is prepared for the volatile oil and ethanol extract of Bupleurum chinense DC.Pharmacological experiment shows that Bupleurum chinense DC difference extract all has apparent antiepileptic action, wherein suitable, volatile oil antiepileptic action is better than Bupleurum Chinese root to ethanol extract antiepileptic action with Bupleurum Chinese root.The antiepileptic efficacy that Bupleurum chinense DC volatile oil and Lamotrigine are used in combination is significantly better than the effect that mentioned component is applied alone, and be significantly better than radix bupleuri compound preparation and antiepileptic efficacy that Lamotrigine is used in combination, for Chinese and Western medicine compound preparation safety and quality controllable provide possibility.)

1. purposes of the Bupleurum Chinese volatile oil in preparation treatment epilepsy drugs, which is characterized in that the volatile oil is for Bupleurum Chinese The volatile oil of the volatile oil of upper part, the Bupleurum chinense DC is prepared as follows: weighing dry Bupleurum Chinese on the ground Part simultaneously crushes, and is extracted by steam distillation, after being dried over anhydrous sodium sulfate to obtain the final product.

2. purposes according to claim 1, which is characterized in that use the extraction time of steam distillation for 12 hours, Institute's water requirement is 5-10 times of medicinal material weight, and the drying time of anhydrous sodium sulfate is 12 hours.

3. purposes of the Bupleurum Chinese ethanol extract in preparation treatment epilepsy drugs, which is characterized in that the ethanol extract is The ethanol extract of Bupleurum chinense DC, the Bupleurum chinense DC ethanol extract are prepared as follows: being weighed dry Dry Bupleurum chinense DC and crushing, the dregs of a decoction of the collection after extraction by steam distillation volatile oil is added ethanol solution and adds Circumfluence distillation, merging filtrate are concentrated under reduced pressure to give Bupleurum chinense DC ethanol extract.

4. purposes according to claim 3, which is characterized in that select 70-95% ethanol solution, solid-liquid ratio 1:2-4 adds Circumfluence distillation 3 times, every time 2 hours.

5. the purposes in preparation treatment epilepsy drugs is used in combination in Bupleurum chinense DC extract and antiepileptic.

6. purposes according to claim 5, which is characterized in that the Bupleurum chinense DC extract is Bupleurum Chinese ground Part ethanol extract or Bupleurum chinense DC volatile oil, the antiepileptic are Lamotrigine.

7. a kind of pharmaceutical composition for treating epilepsy, which is characterized in that the effective component of described pharmaceutical composition is Bupleurum Chinese Upper part volatile oil and Lamotrigine, wherein the content of Bupleurum chinense DC volatile oil and Lamotrigine ratio is 8:1.

Technical field

The present invention relates to medicines, make more particularly to a kind of anti-epileptic of Bupleurum chinense DC difference extract Medical usage.

Background technique

Epilepsy (Epilepsy) is very common one of the nervous system disease in the whole world, and most refractory nerveous system One of disease of uniting, has sudden and repetitious pathological characteristic, and epileptic needs lifelong medication treatment mostly.Epilepsy be by A kind of chronic cerebral functional disorder disease caused by many reasons, with temporary caused by the cerebral neuron over-discharge that repeated When sexual centre nervous function it is not normal with the characteristics of, be main performance with jerk and the loss of consciousness.There are about 70,000,000 in the whole world Epileptic, wherein 90% or more patient is located at low, middle income country.Epidemiological survey shows, the hair of China's epilepsy Sick rate is 5 ‰~7 ‰, existing about 6,500,000~9,100,000 epileptic in the whole nation.The generation of epilepsy can not only cause health problem, Make patient's holistic health low, intelligence and impaired body function, accident occur and injured risk is higher, and is adjoint insane A series of social mentality's problems such as mood, family, the occupation that epilepsy generates, cause tremendous influence to the whole quality of life of patient. In addition, the direct health care costs of epilepsy and because caused by quality of life and production work Disability indirect costs it is huge, make At more heavy financial burden.Therefore, it understands the pathogenesis of epilepsy in depth, seek optimal therapeutic agent and control insane The means of epilepsy all have important meaning to patient and society.

Different types of etiopathogenises can lead to epileptic attack, and pathogenesis is complicated, not yet illustrate completely so far.The treatment means of epilepsy Including carrying out immunotherapy targeted autoantibody, drug therapy, surgical operation therapy and physical therapy etc., usual antiepileptic to the cause of disease It (AEDs) is the preferred means for treating epilepsy, clinically common antiepileptic had both included that conventional antiepileptic drugs such as benzene bar compares Appropriate, dilantin sodium, carbamazepine, sodium vedproate, Clonazepam, ethymal, mysoline etc., also include nearly 20 years both at home and abroad successively Research and develop the new antiepileptic drugs of listing, such as Oxcarbazepine, Lamotrigine, Levetiracetam.But it is with dizziness headache, mutual aid The side effects such as imbalance, allergic reaction, cause some patientss not to be resistant to.However, about 1/3 patient is in drug refractory, it is difficult The property controlled epilepsy generally refers to reasonable application Canonical management 2 years or more (two or more different AEDs by AEDs Use in conjunction and reach effective blood drug concentration), therapeutic effect is still bad, symptom control it is still undesirable, in order to control epilepsy hair Make, operation just becomes another selection.Temporal epilepsy is most common one of epilepsy syndromes, about 50% in treatment of intractable epilepsy It is all this type above.But medial temporal lobe structures (including inside structure) are complicated, before neighbouring thalamus, brain stem, arteria cerebri media, choroid The important anatomy structures such as artery, oculomotor nerve, surgical procedure will cause serious complication accidentally, and the Capital University of Medical Sciences three wins brain Hospital, section functional neurosurgery surgery is cut in the parallel temporal epilepsy stove of 436 patients with temporal epilepsies of in March, 2008 diagnosis and treatment in March, -2016 Except art, wherein 24 (5.5%) postoperative there are the complication such as hemiplegia, intracranial infection, intracranial hematoma, temporalis atrophy.And Chinese medicine is controlled Treating epilepsy has Small side effects, multicomponent, multiple target point and the advantage for reducing epilepsy complication breaking-out, has attracted domestic and international scientific research The concern of person has very wide development prospect.

Bupleurum Chinese root is the dry root of umbelliferae bupleurum (Bupleurum chinense DC.).Radix bupleuri is common solution Medicine administered to bring out the cold is used as China's tradition conventional Chinese medicine, has the application in more than 2000 years.Radix bupleuri is listed in first recorded in Shennong's Herbal Top grade, and be recorded to be summarised in and go through in version " Chinese Pharmacopoeia " and " dictionary of medicinal plant ".Have in inducing diaphoresis, it is soothing the liver, the effect of rising Yang, Be usually used in cold, fever, fevers and chills alternate, malaria, liver depression and qi stagnation, chest and rib distending pain, rectal prolapse, uterine prolapse, irregular menstruation, it is anticonvulsion, Anti-inflammatory, antidepression, anti-epileptic, antitumor etc..Modern pharmacology research shows that bupleurum Chinense volatile oil has antipyretic, anti-inflammatory, liver protection etc. Effect.Saikoside is the main component of Bupleurum Chinese, and has extensive bioactivity, such as anti-inflammatory, antitumor, Hepatoprotective cholagogue, Immunological regulation and central inhibitory action etc..Flavones ingredient in Bupleurum Chinese has the effects that cholagogue, antibacterial, sterilization.For For Bupleurum Chinese treats the present Research of epilepsy, existing research at present shows that bupleurum total saponin, saikosaponin a, c, d, radix bupleuri are waved (radix bupleuri Shugan Decoction, radix bupleuri Shugan Decoction joint guipi decoction add taste, radix bupleuri Shugan Decoction that fritillaria thunbergii is added to add taste plus taste bavin for hair oil and compound Add Os Draconis Concha Ostreae soup etc., Xiao Chaihu Tang recklessly) there is certain effect to treatment epilepsy.Currently, mostly about by radix bupleuri compound preparation The report being used in combination with common anti-epileptic Western medicine, significantly improves the curative effect of epilepsy after combination, and adverse reaction rate is bright Aobvious to reduce, the trend of having become is used in combination in Chinese and Western medicine.

Bupleurum chinense DC records the function for all having promoting the circulation of qi according to " book on Chinese herbal medicine states hook member ", " book on Chinese herbal medicine brief points ", the Materia Medica Effect；Supplement to Thousand Golden Prescriptions, " book on Chinese herbal medicine brief points ", that " Chinese medicine voluminous dictionary " record takes Aerial Parts of Bupleurum chinense DC juice drop ear to control soldier is deaf.Currently, right The pharmacological research of Bupleurum chinense DC is more plain, only to antipyretic liver protection, anti-inflammatory, antiviral, analgesia and enhancing hair The report of tubule function related fields, the report not being used in combination about Bupleurum chinense DC extract and anti-epileptic Western medicine more Road.

Summary of the invention

The object of the present invention is to provide a kind of new drug activities of Bupleurum chinense DC difference extract, specifically It says, the present invention provides a kind of Bupleurum chinense DC difference extracts and anti-epileptic Western medicine to be combined in terms of the drug of anti-epileptic Application.

The present invention is achieved by the following technical solution:

On the one hand, the different extracts that the present invention provides a kind of Bupleurum chinense DC (are mentioned comprising volatile oil compositions, ethyl alcohol Take component) purposes in epilepsy drugs is treated in preparation.

Preferably, the volatile oil of the Bupleurum chinense DC is made of following steps: weighing dry Bupleurum Chinese on the ground Part simultaneously crushes, and is extracted by steam distillation, after being dried over anhydrous sodium sulfate to obtain the final product.

Preferably, use the extraction time of steam distillation for 12 hours, institute's water requirement is 5-10 times of medicinal material weight, The drying time of anhydrous sodium sulfate is 12 hours.

Preferably, the Bupleurum chinense DC ethanol extract is made of following steps: with weighing dry Bupleurum Chinese Upper part simultaneously crushes, and collects the dregs of a decoction after extraction by steam distillation volatile oil, and ethanol solution heating and refluxing extraction is added, and closes And filtrate, it is concentrated under reduced pressure to give Bupleurum chinense DC ethanol extract.

Preferably, selection 70-95% ethanol solution, solid-liquid ratio 1:2-4, heating and refluxing extraction 3 times, every time 2 hours.

Preferably, the drug be using the volatile oil of Bupleurum chinense DC or ethanol extract as active constituent, in addition The preparation that pharmaceutically acceptable auxiliary material or complementary ingredient are prepared.

Preferably, the preparation is tablet, capsule, granule, oral solution or suspension.

Preferably, the different extracts are active constituent.

Preferably, the different extracts include antiepileptic activity substance.

It can a second object of the present invention is to provide make full use of Bupleurum chinense DC resources of medicinal plant abundant Energy.

Preferably, the 87.5% of the total biological yield of Bupleurum chinense DC biological yield Zhan (while being also that Bupleurum Chinese root is raw 7 times of produce amount).

Preferably, the content of Bupleurum chinense DC volatile oil is about 7.8 times of Bupleurum Chinese root.

It (include volatile oil compositions, second third object of the present invention is to provide the different extracts of Bupleurum chinense DC Alcohol extracting component) it is used in combination with anti-epileptic Western medicine and treats the purposes in epilepsy drugs in preparation.

Preferably, anti-epileptic Western medicine is Lamotrigine.

Compared with prior art, the beneficial effects of the present invention are: pharmacological experiment is shown, Bupleurum chinense DC difference is mentioned Take object that there is apparent antiepileptic action, wherein ethanol extract antiepileptic action suitable, volatile oil anti-epileptic with Bupleurum Chinese root Effect is better than Bupleurum Chinese root, and the antiepileptic efficacy that Bupleurum chinense DC volatile oil and Lamotrigine are used in combination is significantly better than list With the effect of mentioned component, and it is significantly better than the antiepileptic efficacy that radix bupleuri compound preparation and Lamotrigine are used in combination, is The safety of compound Western medicine preparation and quality controllable provide possibility.

Specific embodiment

Below in conjunction with specific embodiment, the invention will be further described.It will be understood that following embodiment is merely to illustrate this Invention is not for limiting the scope of the invention.

In the following examples, the experimental methods for specific conditions are not specified, usually according to normal condition or manufacturer provide item Part carries out.