阅读说明：本技术 一种从刺五加中提取刺五加皂苷的提取方法 (method for extracting acanthopanax saponin from acanthopanax senticosus ) 是由 杨成东 于 2018-05-29 设计创作，主要内容包括：本发明涉及一种医药技术领域,具体涉及一种刺五加皂苷的提取方法,其处理步骤包括：(1)将刺五加粉碎,乙醇回流提取；(2)大孔树脂纯化；(3)高速逆流色谱仪分离；(4)浓缩干燥,即得刺五加皂苷。本发明采用乙醇回流提取-大孔树脂纯化-高速逆流色谱分离提取刺五加皂苷,工艺简单、提取时间短、提取率高,且生产成本低,可进行工业化的生产。(the invention relates to the technical field of medicines, in particular to an extraction method of acanthopanax saponin, which comprises the following processing steps: (1) pulverizing radix Acanthopanacis Senticosi, and extracting with ethanol under reflux; (2) purifying by macroporous resin; (3) separating by a high-speed counter-current chromatograph; (4) concentrating and drying to obtain eleutheroside. The method adopts ethanol reflux extraction, macroporous resin purification and high-speed countercurrent chromatography separation to extract the acanthopanax saponin, has simple process, short extraction time, high extraction rate and low production cost, and can be used for industrialized production.)

1. The extraction method of the acanthopanax senticosus saponin is characterized by comprising the following steps:

1) Crushing acanthopanax, extracting with 30-60% ethanol in an amount which is 3-5 times that of the acanthopanax for 1-3 times in a refluxing manner, wherein each time is 0.5-1.5 hours, filtering, combining extracting solutions, and concentrating until no ethanol exists to obtain a concentrated solution;

2) Adding the concentrated solution into a macroporous resin column for adsorption and purification, eluting with 4-8 BV of acid alcohol, collecting eluent, and recovering the solvent until the eluent is dry;

3) separating the obtained extract with high speed countercurrent chromatography, collecting eluate, concentrating, and drying to obtain high purity eleutheroside.

2. The method for extracting eleutheroside as claimed in claim 1, wherein the macroporous resin in step 2) is AB-8, D-101, DS-401, and the volume-to-weight ratio of the dosage to the medicinal material is 1: 1-3.

3. The method for extracting saponins of acanthopanax senticosus as claimed in claim 1, wherein the acid alcohol in step 2) is a 30-50% ethanol solution containing 1% acetic acid.

4. The method for extracting saponins of acanthopanax senticosus of claim 1, wherein the parameters of the high speed counter current chromatograph in the step 3) are set as follows: the rotating speed is 700-1000 r/min, and the flow rate of the mobile phase is 1-5 ml/min.

Technical Field

The invention belongs to the field of separation of active ingredients of natural products, and particularly relates to a method for extracting and purifying acanthopanax saponin from acanthopanax.

Background

acanthopanax senticosus, named as: the kan crutch is sloppy, the sloppy and one hundred needles. Shrubs 1-6 meters high; and (4) multiple branches are formed. The flowering period is 6-7 months, and the fruit period is 8-10 months. Is distributed in Heilongjiang, Jilin, Liaoning, Hebei and Shanxi in China. Living in hillside forests and roadside irrigation clusters; the medicine nursery is usually cultivated. Acanthopanax senticosus is sweet and warm, enters spleen and lung channels, and can tonify spleen and qi, tonify lung qi, and treat spleen and lung qi deficiency, weakness and hypodynamia, inappetence, loose stool, shortness of breath and no speaking desire. The main medicinal component is acanthopanax saponin, and the extraction process of industrially produced acanthopanax saponin is not complete, so that the purity and the industrial production benefit of the product are directly influenced. The research is mainly carried out on the method, and the reference is provided for improving the purity of the product and the industrial production benefit. Therefore, the research on the extraction process of the acanthopanax saponin is of great significance to the deep development and utilization of the acanthopanax.

The extraction of eleutheroside is carried out by conventional alkalization extraction method. The traditional extraction method has the defects of large organic solvent usage amount, serious pollution, low extraction rate and the like, and the acanthopanax saponin is separated by adopting a high-speed counter-current chromatograph, so that the plant tissue can be crushed and decomposed mildly, the yield is improved greatly, and the three-dimensional structure of the components is not easy to damage.

disclosure of Invention

The invention aims to provide a method for extracting eleutheroside with short extraction time and high yield aiming at the defects of the prior art.

The invention comprises the following steps:

Crushing acanthopanax senticosus, performing reflux extraction for 1-3 times by using 30-60% ethanol in an amount which is 3-5 times that of the acanthopanax senticosus for 0.5-1.5 h each time, filtering, combining extracting solutions, concentrating until no alcohol exists to obtain a concentrated solution, adding the concentrated solution into a macroporous resin column for adsorption and purification, eluting by using 4-8 BV acid alcohol, collecting eluent, recovering a solvent to dryness, separating the acanthopanax senticosus saponin extract by using a high-speed counter-current chromatograph, collecting the eluent, concentrating and drying to obtain high-purity acanthopanax senticosus saponin.

The macroporous resin is AB-8, D-101 and DS-401, and the volume weight ratio of the dosage to the medicinal material is 1: 1-3.

the acid alcohol is a 30-50% ethanol solution containing 1% acetic acid.

The parameters of the high-speed countercurrent chromatograph are set as follows: the rotating speed is 700-1000 r/min, and the flow rate of the mobile phase is 1-5 ml/min.

The present invention will be further described with reference to specific embodiments, but the scope of the invention as claimed is not limited to the following embodiments.

The specific implementation mode is as follows: