阅读说明：本技术 一种发酵生产酒精的方法 (Method for producing alcohol by fermentation ) 是由 郑哲 于 2021-08-05 设计创作，主要内容包括：本发明提供一种发酵生产酒精的方法,包括以下步骤：S1.将玉米原料粉碎为玉米粉,过筛网,将筛下物作为调成粉浆的原料,然后与水配成粉浆；S2.将所述粉浆中加入第一α-淀粉酶,然后在55℃-60℃下进行第一次液化处理,得到第一液化浆料；S3.将所述第一液化浆料加入第二α-淀粉酶,然后在70℃-80℃下进行第二次液化处理,得到第二液化浆料S4.将所述第二液化浆料加入第三α-淀粉酶,并加入稳定剂,然后在95℃-105℃下进行第三次液化处理,得到第三液化浆料；S5.将所述第三液化浆料中加入糖化酶,并加入酵母料和发酵促进剂进行发酵,经发酵后的醪液进行蒸馏,得到酒精。本发明的发酵生产酒精的方法,生产效率高,具有高出酒率,较低的还原糖及残总糖含量。(The invention provides a method for producing alcohol by fermentation, which comprises the following steps: s1, crushing a corn raw material into corn flour, screening the corn flour by using a screen, taking undersize products as raw materials for preparing starch slurry, and then preparing the starch slurry with water; s2, adding a first alpha-amylase into the slurry, and then carrying out first liquefaction treatment at 55-60 ℃ to obtain first liquefied slurry; s3, adding a second alpha-amylase into the first liquefied slurry, then carrying out second liquefaction treatment at 70-80 ℃ to obtain a second liquefied slurry S4, adding a third alpha-amylase into the second liquefied slurry, adding a stabilizer, and then carrying out third liquefaction treatment at 95-105 ℃ to obtain a third liquefied slurry; s5, adding saccharifying enzyme into the third liquefied slurry, adding a fermentation master batch and a fermentation promoter for fermentation, and distilling fermented liquor to obtain alcohol. The method for producing alcohol by fermentation has high production efficiency, high wine yield and lower reducing sugar and residual total sugar content.)

1. A method for producing alcohol by fermentation, which is characterized in that: the method comprises the following steps:

s1, crushing a corn raw material into corn flour, screening the corn flour by using a screen with 30-40 meshes, taking undersize as a raw material for preparing flour slurry, and preparing the flour slurry with water;

s2, adding a first alpha-amylase into the slurry, and then carrying out first liquefaction treatment at 55-60 ℃ to obtain first liquefied slurry;

s3, adding a second alpha-amylase into the first liquefied slurry, and then performing second liquefaction treatment at the temperature of 70-80 ℃ to obtain a second liquefied slurry;

s4, adding a third alpha-amylase into the second liquefied slurry, adding a stabilizer, and performing third liquefaction treatment at 95-105 ℃ to obtain a third liquefied slurry;

s5, adding saccharifying enzyme into the third liquefied slurry, adding a fermentation master batch and a fermentation promoter for fermentation, and distilling fermented liquor to obtain alcohol.

2. The method of fermentative production of alcohol according to claim 1, characterized in that: the concentration of the powder slurry of the step S1 is 36-40 wt%.

3. The method of fermentative production of alcohol according to claim 1, characterized in that: in the step S2, the source of the first alpha-amylase is fungi, and the adding amount of the first alpha-amylase is 14-16 u/g; the first liquefaction time is 20-30 min.

4. The method of fermentative production of alcohol according to claim 1, characterized in that: in the step S3, the source of the second alpha-amylase is Bacillus subtilis, and the adding amount of the second alpha-amylase is 18-22 u/g; the second liquefaction time is 15-25 min.

5. The method of fermentative production of alcohol according to claim 1, characterized in that: in the step S4, the source of the third alpha-amylase is Bacillus licheniformis, and the addition amount of the third alpha-amylase is 20-25 u/g; the third liquefaction time is 10-40 min.

6. The method of fermentative production of alcohol according to claim 1, characterized in that: the stabilizing agents in the step S4 are calcium citrate and epsilon-polylysine, the adding amount of the calcium citrate is 0.02-0.06% of the total mass of the second liquefied slurry, and the adding amount of the L-arginine is 0.01-0.04% of the total mass of the second liquefied slurry.

7. The method of fermentative production of alcohol according to claim 1, characterized in that: the addition amount of the saccharifying enzyme in the step S5 is 200-300U/g.

8. The method of fermentative production of alcohol according to claim 1, characterized in that: the addition amount of the yeast material of the step S5 is 0.4-0.8 ml/g.

9. The method of fermentative production of alcohol according to claim 1, characterized in that: the fermentation accelerator in the step S5 comprises indolebutyric acid, magnesium sulfate and ammonium bicarbonate, wherein the addition amount of the indolebutyric acid is 0.01-0.1% of the total mass of the third liquefied slurry, the addition amount of the magnesium sulfate is 0.04-0.4% of the total mass of the third liquefied slurry, and the addition amount of the ammonium bicarbonate is 0.002-0.02% of the total mass of the third liquefied slurry.

10. The method of fermentative production of alcohol according to claim 1, characterized in that: the fermentation temperature of the step S5 is 30-37 ℃, and the fermentation time is 48-96 h.

Technical Field

The invention relates to the technical field of alcohol, in particular to a method for producing alcohol by fermentation.

Background

Alcohol is used as a renewable strategic resource and enters into industrial products which are intensively developed in China, but the alcohol fermentation industry is the industry which has great pollution to the environment at present. Most of the wastewater generated by the traditional fermentation method is discharged into natural water after being treated, and although various indexes such as COD (chemical oxygen demand) and the like in the discharged water after being treated reach the standard, the difference of the discharged water and the natural water is still large. The alcohol fermentation industry is a bulk product production industry, and a discharged water metering unit is usually ten thousand tons, so that the discharged water still has great pollution to natural water. The existing method for producing alcohol by fermentation has low production efficiency and high cost.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides the method for producing the alcohol by fermentation, which can improve the production efficiency of the alcohol, reduce the cost and improve the wine yield. The technical scheme adopted by the invention is as follows:

a method of producing alcohol by fermentation, wherein: the method comprises the following steps:

s1, crushing a corn raw material into corn flour, screening the corn flour by using a screen with 30-40 meshes, taking undersize as a raw material for preparing flour slurry, and preparing the flour slurry with water;

s2, adding a first alpha-amylase into the slurry, and then carrying out first liquefaction treatment at 55-60 ℃ to obtain first liquefied slurry;

s3, adding a second alpha-amylase into the first liquefied slurry, and then performing second liquefaction treatment at the temperature of 70-80 ℃ to obtain a second liquefied slurry;

s4, adding a third alpha-amylase into the second liquefied slurry, adding a stabilizer, and performing third liquefaction treatment at 95-105 ℃ to obtain a third liquefied slurry;

s5, adding saccharifying enzyme into the third liquefied slurry, adding a fermentation master batch and a fermentation promoter for fermentation, and distilling fermented liquor to obtain alcohol.

When the alpha-amylase acts on amylose, the two stages are divided into two stages, the first stage can hydrolyze the amylose into maltose, maltotriose and amylose maltooligosaccharide, the second stage has lower speed and hydrolyzes the maltotriose and the maltooligosaccharide into maltose and glucose, and the speed and the hydrolysis completeness of the maltotriose and the maltose oligosaccharide into maltose and glucose are improved through three times of liquefaction.

Preferably, the method for producing alcohol by fermentation, wherein: the concentration of the powder slurry of the step S1 is 36-40 wt%.

Preferably, the method for producing alcohol by fermentation, wherein: in the step S2, the source of the first alpha-amylase is fungi, and the adding amount of the first alpha-amylase is 14-16 u/g; the first liquefaction time is 20-30 min.

Preferably, the method for producing alcohol by fermentation, wherein: in the step S3, the source of the second alpha-amylase is Bacillus subtilis, and the adding amount of the second alpha-amylase is 18-22 u/g; the second liquefaction time is 15-25 min.

Preferably, the method for producing alcohol by fermentation, wherein: in the step S4, the source of the third alpha-amylase is Bacillus licheniformis, and the addition amount of the third alpha-amylase is 20-25 u/g; the third liquefaction time is 10-40 min.

Preferably, the method for producing alcohol by fermentation, wherein: the stabilizing agents in the step S4 are calcium citrate and epsilon-polylysine, the adding amount of the calcium citrate is 0.02-0.06% of the total mass of the second liquefied slurry, and the adding amount of the L-arginine is 0.01-0.04% of the total mass of the second liquefied slurry.

Preferably, the method for producing alcohol by fermentation, wherein: the addition amount of the saccharifying enzyme in the step S5 is 200-300U/g.

Preferably, the method for producing alcohol by fermentation, wherein: the addition amount of the yeast material of the step S5 is 0.4-0.8 ml/g.

Preferably, the method for producing alcohol by fermentation, wherein: the fermentation accelerator in the step S5 comprises indolebutyric acid, magnesium sulfate and ammonium bicarbonate, wherein the addition amount of the indolebutyric acid is 0.01-0.1% of the total mass of the third liquefied slurry, the addition amount of the magnesium sulfate is 0.04-0.4% of the total mass of the third liquefied slurry, and the addition amount of the ammonium bicarbonate is 0.002-0.02% of the total mass of the third liquefied slurry.

The nitrogen source is an important raw material for synthesizing nitrogen-containing substances such as mycoprotein, nucleic acid and the like, the existing level of the nitrogen source is directly related to the anabolism of the yeast, and the ammonium bicarbonate provides the nitrogen source for the yeast and provides nutrition required by the yeast, so that the fermentation activity is improved, the fermentation rate is improved, the fermentation is thorough, the residual sugar is low, and the alcohol conversion rate is improved.

The insufficient magnesium element can prevent fermentation or make fermentation incomplete, and in the fermentation process, the yeast often has nutrition fermentation activity because of lack of nutrition and alcohol accumulation, the magnesium element is properly added in the fermentation process, the yeast growth and fermentation are facilitated, the magnesium source in the corn flour is less, the proper magnesium source is added, the yeast growth and fermentation are facilitated, and the mash with high initial magnesium source concentration is obtained. Not only the cell quantity in the mash is large, but also the fermentation speed is high and the fermentation is thorough. Researches show that the addition of indolebutyric acid has a certain promotion effect on fermentation, and the indole butyric acid, magnesium sulfate and ammonium bicarbonate are synergistically used as the fermentation promoter, so that the process can obtain the highest alcohol content and lower reducing sugar and residual total sugar content.

Preferably, the method for producing alcohol by fermentation, wherein: the fermentation temperature of the step S5 is 30-37 ℃, and the fermentation time is 48-96 h.

The invention has the advantages that:

(1) the method for producing alcohol by fermentation has high production efficiency, high wine yield and lower reducing sugar and residual total sugar content.

(2) According to the method for producing alcohol by fermentation, disclosed by the invention, the speed and the hydrolysis thoroughness of the maltotriose and maltooligosaccharide hydrolyzed into maltose and glucose are improved by three times of liquefaction; indole butyric acid, magnesium sulfate and ammonium bicarbonate are added in the fermentation to be used as a fermentation promoter in a synergistic manner, so that the production method can obtain the highest alcohol content and lower reducing sugar and residual total sugar content.

Detailed Description

The present invention will be further described with reference to the following specific examples.

Example 1

A method of producing alcohol by fermentation, wherein: the method comprises the following steps:

s1, crushing a corn raw material into corn flour, sieving the corn flour by a 30-40-mesh sieve, taking undersize as a raw material for preparing starch slurry, and preparing the starch slurry and water into 36 wt% starch slurry;

s2, adding a first alpha-amylase into the powder slurry, and then carrying out primary liquefaction treatment for 30min at 55 ℃, wherein the source of the first alpha-amylase is fungi, and the addition amount of the first alpha-amylase is 14u/g, so as to obtain a first liquefied slurry;

s3, adding the first liquefied slurry into second alpha-amylase, wherein the source of the second alpha-amylase is bacillus subtilis, and the adding amount of the second alpha-amylase is 18 u/g; then carrying out secondary liquefaction treatment at 70 ℃ for 25min to obtain second liquefied slurry;

s4, adding a third alpha-amylase into the second liquefied slurry, wherein the source of the third alpha-amylase is bacillus licheniformis, the adding amount of the third alpha-amylase is 20u/g, adding a stabilizer which is calcium citrate and epsilon-polylysine, the adding amount of the calcium citrate is 0.02% of the total mass of the second liquefied slurry, the adding amount of the L-arginine is 0.01% of the total mass of the second liquefied slurry, and then carrying out third liquefaction treatment for 40min at 95 ℃ to obtain a third liquefied slurry;

s5, adding saccharifying enzyme into the third liquefied slurry, wherein the adding amount of the saccharifying enzyme is 200U/g, adding 0.4ml/g of yeast material and a fermentation promoter for fermentation at 30 ℃ for 96 hours, wherein the fermentation promoter comprises indolebutyric acid, magnesium sulfate and ammonium bicarbonate, the adding amount of the indolebutyric acid is 0.01% of the total mass of the third liquefied slurry, the adding amount of the magnesium sulfate is 0.04% of the total mass of the third liquefied slurry, the adding amount of the ammonium bicarbonate is 0.002% of the total mass of the third liquefied slurry, and distilling fermented mash to obtain alcohol.

Example 2

A method of producing alcohol by fermentation, wherein: the method comprises the following steps:

s1, crushing a corn raw material into corn flour, sieving the corn flour by a 30-40-mesh sieve, taking undersize as a raw material for preparing starch slurry, and preparing the starch slurry and water into 37 wt% starch slurry;

s2, adding a first alpha-amylase into the powder slurry, wherein the source of the first alpha-amylase is fungi, the adding amount of the first alpha-amylase is 15u/g, and then carrying out primary liquefaction treatment at 58 ℃ for 24min to obtain a first liquefied slurry;

s3, adding a second alpha-amylase into the first liquefied slurry, wherein the source of the second alpha-amylase is bacillus subtilis, and the adding amount of the second alpha-amylase is 20u/g, and then carrying out secondary liquefaction treatment at 76 ℃ for 20min to obtain a second liquefied slurry;

s4, adding a third alpha-amylase into the second liquefied slurry, wherein the source of the third alpha-amylase is bacillus licheniformis, and the addition amount of the third alpha-amylase is 22 u/g; adding a stabilizer which is calcium citrate and epsilon-polylysine, wherein the adding amount of the calcium citrate is 0.05 percent of the total mass of the second liquefied slurry, and the adding amount of the L-arginine is 0.02 percent of the total mass of the second liquefied slurry, and then carrying out third liquefaction treatment for 20min at 100 ℃ to obtain third liquefied slurry;

s5, adding 220U/g saccharifying enzyme into the third liquefied slurry, adding 0.5ml/g of fermentation master batch and a fermentation promoter for fermentation at the fermentation temperature of 32 ℃ for 56 hours, wherein the fermentation promoter comprises indolebutyric acid, magnesium sulfate and ammonium bicarbonate, the addition amount of the indolebutyric acid is 0.05 percent of the total mass of the third liquefied slurry, the addition amount of the magnesium sulfate is 0.1 percent of the total mass of the third liquefied slurry, the addition amount of the ammonium bicarbonate is 0.01 percent of the total mass of the third liquefied slurry, and distilling fermented mash to obtain alcohol.

Example 3

A method of producing alcohol by fermentation, wherein: the method comprises the following steps:

s1, crushing a corn raw material into corn flour, sieving the corn flour by a 30-40-mesh sieve, taking undersize as a raw material for preparing flour slurry, and preparing the flour slurry with water into 40 wt% of flour slurry;

s2, adding a first alpha-amylase into the powder slurry, wherein the source of the first alpha-amylase is fungi, the adding amount of the first alpha-amylase is 16u/g, and then carrying out primary liquefaction treatment at 60 ℃ for 20min to obtain a first liquefied slurry;

s3, adding a second alpha-amylase into the first liquefied slurry, wherein the source of the second alpha-amylase is bacillus subtilis, and the adding amount of the second alpha-amylase is 22u/g, and then carrying out secondary liquefaction treatment for 15min at 80 ℃ to obtain a second liquefied slurry;

s4, adding a third alpha-amylase into the second liquefied slurry, wherein the source of the third alpha-amylase is bacillus licheniformis, the adding amount of the third alpha-amylase is 25u/g, adding a stabilizer which is calcium citrate and epsilon-polylysine, the adding amount of the calcium citrate is 0.06% of the total mass of the second liquefied slurry, the adding amount of the L-arginine is 0.04% of the total mass of the second liquefied slurry, and then carrying out third liquefaction treatment for 10min at 105 ℃ to obtain a third liquefied slurry;

s5, adding 300U/g saccharifying enzyme into the third liquefied slurry, adding 0.8ml/g of fermentation master batch and a fermentation promoter for fermentation at 37 ℃ for 48 hours, wherein the fermentation promoter comprises indolebutyric acid, magnesium sulfate and ammonium bicarbonate, the addition of the indolebutyric acid is 0.1% of the total mass of the third liquefied slurry, the addition of the magnesium sulfate is 0.4% of the total mass of the third liquefied slurry, the addition of the ammonium bicarbonate is 0.02% of the total mass of the third liquefied slurry, and distilling fermented mash to obtain alcohol.

Comparative example 1

A method of producing alcohol by fermentation, wherein: the method comprises the following steps:

s1, crushing a corn raw material into corn flour, sieving the corn flour by a 30-40-mesh sieve, taking undersize as a raw material for preparing starch slurry, and preparing the starch slurry and water into 36 wt% starch slurry;

s2, adding a first alpha-amylase into the powder slurry, and then carrying out primary liquefaction treatment for 30min at 55 ℃, wherein the source of the first alpha-amylase is fungi, and the addition amount of the first alpha-amylase is 14u/g, so as to obtain a first liquefied slurry;

s3, adding the first liquefied slurry into second alpha-amylase, wherein the source of the second alpha-amylase is bacillus subtilis, and the adding amount of the second alpha-amylase is 18 u/g; then carrying out secondary liquefaction treatment at 70 ℃ for 25min to obtain second liquefied slurry;

s4, adding a third alpha-amylase into the second liquefied slurry, wherein the source of the third alpha-amylase is bacillus licheniformis, the adding amount of the third alpha-amylase is 20u/g, and then carrying out third liquefaction treatment for 40min at 95 ℃ to obtain a third liquefied slurry;

s5, adding saccharifying enzyme into the third liquefied slurry, wherein the adding amount of the saccharifying enzyme is 200U/g, adding 0.4ml/g of yeast material and a fermentation promoter for fermentation at 30 ℃ for 96 hours, wherein the fermentation promoter comprises indolebutyric acid, magnesium sulfate and ammonium bicarbonate, the adding amount of the indolebutyric acid is 0.01% of the total mass of the third liquefied slurry, the adding amount of the magnesium sulfate is 0.04% of the total mass of the third liquefied slurry, the adding amount of the ammonium bicarbonate is 0.002% of the total mass of the third liquefied slurry, and distilling fermented mash to obtain alcohol.

Comparative example 2

A method of producing alcohol by fermentation, wherein: the method comprises the following steps:

s1, crushing a corn raw material into corn flour, sieving the corn flour by a 30-40-mesh sieve, taking undersize as a raw material for preparing starch slurry, and preparing the starch slurry and water into 37 wt% starch slurry;

s2, adding a first alpha-amylase into the powder slurry, wherein the source of the first alpha-amylase is fungi, the adding amount of the first alpha-amylase is 15u/g, and then carrying out primary liquefaction treatment at 58 ℃ for 24min to obtain a first liquefied slurry;

s3, adding 220U/g saccharifying enzyme into the third liquefied slurry, adding 0.5ml/g of yeast master batch and a fermentation promoter for fermentation at the temperature of 32 ℃ for 56 hours, wherein the fermentation promoter comprises indolebutyric acid, magnesium sulfate and ammonium bicarbonate, the addition amount of the indolebutyric acid is 0.05 percent of the total mass of the third liquefied slurry, the addition amount of the magnesium sulfate is 0.1 percent of the total mass of the third liquefied slurry, the addition amount of the ammonium bicarbonate is 0.01 percent of the total mass of the third liquefied slurry, and distilling fermented mash to obtain alcohol.

Comparative example 3

A method of producing alcohol by fermentation, wherein: the method comprises the following steps:

s1, crushing a corn raw material into corn flour, sieving the corn flour by a 30-40-mesh sieve, taking undersize as a raw material for preparing flour slurry, and preparing the flour slurry with water into 40 wt% of flour slurry;

s2, adding a first alpha-amylase into the powder slurry, wherein the source of the first alpha-amylase is fungi, the adding amount of the first alpha-amylase is 16u/g, and then carrying out primary liquefaction treatment at 60 ℃ for 20min to obtain a first liquefied slurry;

s3, adding a second alpha-amylase into the first liquefied slurry, wherein the source of the second alpha-amylase is bacillus subtilis, and the adding amount of the second alpha-amylase is 22u/g, and then carrying out secondary liquefaction treatment for 15min at 80 ℃ to obtain a second liquefied slurry;

s4, adding a third alpha-amylase into the second liquefied slurry, wherein the source of the third alpha-amylase is bacillus licheniformis, the adding amount of the third alpha-amylase is 25u/g, adding a stabilizer which is calcium citrate and epsilon-polylysine, the adding amount of the calcium citrate is 0.06% of the total mass of the second liquefied slurry, the adding amount of the L-arginine is 0.04% of the total mass of the second liquefied slurry, and then carrying out third liquefaction treatment for 10min at 105 ℃ to obtain a third liquefied slurry;

s5, adding 300U/g saccharifying enzyme into the third liquefied slurry, adding 0.8ml/g yeast material, fermenting at 37 ℃ for 48 hours, and distilling fermented mash to obtain alcohol. The results of the performance tests of examples and comparative examples are shown below, and the results are shown in Table 1

TABLE 1

Comparing examples 1-3 with comparative examples 1-3, it can be seen that the ethanol content in the fermentation broth obtained by the production method of the present invention was 16.4% (v/v) or more.

Finally, it should be noted that the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to examples, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.