阅读说明：本技术 一株烈性沙门氏菌噬菌体及其应用 (Salmonella furciosus bacteriophage and application thereof ) 是由 杜新永 马如霞 杨为强 李先胜 崔耐 王立坤 王晓铃 于 2021-05-18 设计创作，主要内容包括：本发明公开了一株烈性沙门氏菌噬菌体,保藏编号为CGMCC No.21996,对全基因进行测序后发现,不具有毒力基因和溶原性基因；该沙门氏菌噬菌体RDP-SA-20051在80℃高温条件下作用30min,效价保持在1.0×10～(8)pfu/mL以上,具有良好的耐高温特性；该噬菌体在pH在5-9范围作用6小时,其效价保持在1×10～(10)pfu/mL之上,在pH＝12以及pH＝2时,仍有噬菌体存活,该噬菌体的酸碱耐受范围较宽；裂解谱广,可裂解不同血清型的沙门氏菌,裂解率达98.9％,具有更强的裂解性能,在治疗沙门氏菌感染疾病具有很好的优势,效价高,防治效果好。(The invention discloses a virulent salmonella bacteriophage with the preservation number of CGMCC No.21996, which is found to have no virulence genes and lysogenic genes after sequencing of all genes; the Salmonella bacteriophage RDP-SA-20051 acts at 80 deg.C for 30min, and the titer is maintained at 1.0 × 10 8 pfu/mL or more, and has good high temperature resistance; the phage was at pH 5-9 range of action for 6 hours, the titer remains at 1X 10 10 Above pfu/mL, at pH 12 and pH 2, the phage still survived, which phage has a wide range of acid-base tolerance; the cracking spectrum is wide, the salmonella of different serotypes can be cracked, the cracking rate reaches 98.9 percent, the cracking performance is stronger, the advantages of treating salmonella infection diseases are good, the titer is high, and the prevention and treatment effect is good.)

1. A virulent salmonella phage is characterized in that the phage is salmonella phage RDP-SA-20051 with the preservation number of CGMCC No. 21996.

2. The virulent salmonella phage of claim 1, wherein the salmonella phage RDP-SA-20051 has a regular hexagonal head with a diameter of about 52nm and a tail length of 110nm, as observed by transmission electron microscopy, and is a long tail phage.

3. The virulent Salmonella phage of claim 1, wherein the Salmonella phage RDP-SA-20051 is subjected to a high temperature condition of 80 ℃ for 30min, and the titer is maintained at 1.0X 10⁸pfu/mL or more, and has good high temperature resistance; the phage is applied at pH 5-9 for 6 hr, and its titer is maintained at 1 × 10¹⁰Above pfu/mL, at pH 12 and pH 2, the phage still survived, which was acid-base tolerant over a wide range.

4. The virulent salmonella phage of claim 1, wherein the salmonella phage RDP-SA-20051 has a broad spectrum of salmonella killing capabilities.

5. Use of the virulent salmonella phage RDP-SA-20051 according to any one of claims 1 to 4 for the preparation of a medicament for the prevention and or treatment of salmonella-caused diseases.

6. Use of the virulent salmonella phage RDP-SA-20051 according to any one of claims 1 to 4 for the preparation of a feed for the prevention and or treatment of salmonella-caused diseases.

7. A germicidal composition for the control of salmonella comprising an effective amount of the salmonella phage RDP-SA-20051 of claim 1.

8. Use of the salmonella phage according to any one of claims 1 to 4, wherein the salmonella phage RDP-SA-20051 is used as a potential therapeutic drug or nutraceutical for bacterial infections caused by salmonella.

9. An additive for preventing and or treating diseases caused by salmonella, comprising the salmonella phage RDP-SA-20051 according to claim 1 as an active ingredient.

10. A cleaning or disinfecting agent comprising the salmonella phage RDP-SA-20051 of claim 1.

Technical Field

The invention relates to the technical field of biology, in particular to a virulent salmonella bacteriophage and application thereof in pathogenic salmonella infection of poultry animals.

Background

The salmonellosis is one of the zoonosis which has important significance in public health, and is a common food-borne pathogenic bacterium. Chickens are the most important hosts of salmonella, which can cause acute or chronic disease in chickens, and chickens infected with salmonella can also cause vertical transmission of salmonella by producing eggs with bacteria, thereby forming a vicious circle. When the chicken outbreak of the salmonellosis occurs, the death rate can reach 80 percent, and huge economic loss is caused to the poultry industry. Once a person ingests animal food containing a large amount of salmonella, food-borne poisoning may be caused, and it is counted that food poisoning caused by salmonella is often listed among bacterial food poisoning in various countries in the world. Antibiotics have been widely used as the first choice for the treatment of bacterial infections. However, with the widespread use and even abuse of antibiotics, the problem of antibiotic resistance of bacteria is now serious, and antibiotic resistance is threatening global health.

Bacteriophages (bacteriophages or Phage) are one of the most abundant organisms in nature, with about 10 being deposited on the earth³¹I.e. containing up to 10 per 1g of soil or water on average⁸A number of phages. It has long been recognized as useful as a therapeutic agent and as a bactericide. Phage pairBacterial strains are highly specific, depend on the molecular structure and complementarity of phage-adsorbed organs and receptors on the surface of recipient bacteria, and do not affect the normal operation of the organism or endogenous tissues. In addition, even with the development of bacterial resistance, phage will remain in large numbers. The use of bacteriophages for the detection and control of the growth of pathogenic and spoilage bacteria in food and environments has received increasing attention from researchers over the past few years and has been supported by a number of studies. Bmvko LYu and the like discuss the advantages and disadvantages of phage for detecting pathogenic bacteria, and point out that the phage has great application prospect in detecting pathogenic bacteria in the aspects of food safety, processing and manufacturing processes and the like. For example, in foods such as sliced honeydew melon, chicken breast, Chinese cabbage, milk, chicken skin, and bean sprout, the amount of salmonella is significantly reduced by using the phage preparation. In addition, salmonella phages have been isolated from various foods such as meat products such as ground beef, pork, etc., dairy products, lettuce, etc., and thus, the phages are natural microorganisms in food products. The experiment collects samples from diseased chicken bodies in modern broiler farms, screens salmonella virulent phages capable of cracking multiple strains of pathogenic bacteria, and analyzes the biological characteristics of the salmonella virulent phages so as to provide theoretical basis for the research of preventing and controlling salmonella by the phages.

Disclosure of Invention

The invention aims to provide a virulent salmonella phage RDP-SA-20051, and aims to solve the problems that poultry in a farm are not treated timely due to salmonella infection and acute morbidity, and the medication is ineffective due to drug resistance of pathogenic bacteria and the like.

In order to achieve the purpose, the invention adopts the following technical scheme:

a virulent salmonella phage RDP-SA-20051 is observed through an electron microscope, the head is in a regular hexagon shape, the diameter of the head is about 52nm, the length of the tail is 110nm, the salmonella phage RDP-SA-20051 is a long-tail phage, through determination, the salmonella phage RDP-SA-20051 does not have virulence genes and lysogenic genes, the phage is preserved in the China general microbiological culture Collection center in 25.03.20.2021, the preservation address is as follows: no. 3 of Xilu No. 1 of Beijing, Chaoyang, and the preservation number is CGMCC No. 21996.

Further, the Salmonella bacteriophage RDP-SA-20051 acts for 30min at 80 deg.C, and the titer is maintained at 1.0 × 10⁸pfu/mL or more, and has good high temperature resistance; the phage is applied at pH 5-9 for 6 hr, and its titer is maintained at 1 × 10¹⁰Above pfu/mL, has significant alkalophilicity; at pH 12 and pH 2, the phage still survived, and the range of acid-base tolerance of the phage was broad.

Further, the Salmonella phage RDP-SA-20051 has a broad spectrum of Salmonella killing ability.

Another purpose of the invention is to provide the application of the salmonella phage RDP-SA-20051 in preparing medicines for preventing and/or treating diseases caused by salmonella.

Another object of the present invention is to provide the use of the Salmonella bacteriophage RDP-SA-20051 for the preparation of a feed for the prevention and or treatment of Salmonella-caused diseases.

The invention also provides a bactericidal composition for preventing and treating salmonella, which comprises an effective amount of salmonella phage RDP-SA-20051.

Another object of the present invention is to provide a use of Salmonella bacteriophage RDP-SA-20051 for providing potential therapeutic drugs or health products for bacterial infection caused by Salmonella.

Another object of the present invention is to provide an additive for preventing and or treating diseases caused by Salmonella by including the Salmonella bacteriophage RDP-SA-20051 as an effective ingredient thereof.

The invention also provides a cleaning agent or disinfectant, which comprises the salmonella phage RDP-SA-20051.

The invention has the beneficial effects that:

(1) the invention separates salmonella BS-20062 from the body of the chicken died of illness in Shandong one farm, and confirms the virulent toxicity and drug resistance of BS-20062 through experiments, and separates a virulent salmonella phage from the environment by taking BS-20062 as a host, the phage RDP-SA-20051 has strong cracking effect on pathogenic salmonella BS-20062 in the poultry breeding environment, and provides a phage source for the industrial production of phage for preventing and controlling pathogenic salmonella BS-20062 in the poultry breeding environment;

(2) the salmonella bacteriophage RDP-SA-20051 provided by the invention is a virulent bacteriophage separated from nature, and toxicological experiments prove that the salmonella bacteriophage RDP-SA-20051 is safe and has no side effect; the test phage does not contain virulence genes or undesirable genes; the invention does not carry out any genetic modification on the test phage;

(3) the Salmonella bacteriophage RDP-SA-20051 acts for 30min at the high temperature of 80 ℃, and the titer is kept at 1.0 multiplied by 10⁸pfu/mL or more, and has good high temperature resistance; the phage is applied at pH 5-9 for 6 hr, and its titer is maintained at 1 × 10¹⁰Above pfu/mL, at pH 12 and pH 2, the phage still survived, which phage has a wide range of acid-base tolerance;

(4) the salmonella bacteriophage RDP-SA-20051 has a wide cracking spectrum, can crack salmonella enteritidis, salmonella gallinarum, salmonella thopson, salmonella typhimurium, salmonella deflurden, salmonella stainer and other salmonella of different serotypes, has a cracking rate of 98.9 percent, has stronger cracking performance, has good advantages in treating salmonella infection diseases, and has high titer and good prevention and treatment effect;

(5) the salmonella bacteriophage RDP-SA-20051 provided by the invention can be used as an effective component of various products applied to environmental disinfection, for example, the salmonella bacteriophage RDP-SA-20051 can be used for disinfecting and decontaminating surfaces of water distribution systems, medical facilities, breeding facilities, public and private facilities or other environments in the forms of liquid soaking, spraying, combined use with aqueous carriers and the like, and can effectively control the growth and activity of target bacteria; the liquid soaking, spraying forms include but are not limited to detergents, disinfectants, detergents, etc.; the aqueous carrier includes but is not limited to phosphate buffer, LB medium, chloride free water, etc.;

(6) the salmonella phage RDP-SA-20051 of the present invention can be used to prepare compositions, reagents, or kits; the kit can be applied to the rapid detection of the salmonella, and the detection comprises but is not limited to the detection of the salmonella in a target sample in the forms of test paper, a kit and the like, or the screening of target pathogenic bacteria in a clinical sample, so that the detection sensitivity can be effectively ensured;

(7) the salmonella bacteriophage RDP-SA-20051 can be used singly or in a mixed way and used as a biological bactericide, a feed additive or a therapeutic drug, a health product and a medical appliance for bacterial infection caused by salmonella; the salmonella bacteriophage RDP-SA-20051 can be applied to treating or preventing infectious diseases caused by salmonella; hosts that can be infected by Salmonella include humans, livestock (pigs, cattle, sheep, etc.), poultry (chickens, ducks, geese, etc.), and various beasts, fishes, rats, etc.;

(8) the salmonella bacteriophage RDP-SA-20051 can be applied to industrial production, can be specifically amplified by host bacteria, can be highly purified by applying a standard virus purification method, and has excellent popularization prospect; according to the description of the invention and the common knowledge in the field, the technicians in the field can prepare the salmonella phage RDP-SA-20051 or the composition thereof into various products applied to the aspects of medical treatment, detection, disinfection, food protection and the like for industrial application; the product form can include, but is not limited to, the carrier carrying, the concentrated injection or the medicament soaking and the like, and the product is applied to the controlled host body surface, the oral part, the rectum, the inner pleura and other parts; as one embodiment, the carrier-borne form includes, but is not limited to, oral aqueous carriers, oral anhydrous carriers, cream formulations, and the like; concentrated injection forms include, but are not limited to, vaccine injections, pleural cavity injections, transvenous injections, etc.; dosage soaking forms include, but are not limited to, aerosols, rinses, and the like.

Drawings

The above and other features, properties and advantages of the present invention will become more apparent from the following description of the embodiments with reference to the accompanying drawings:

FIG. 1 is a diagram showing the result of PCR amplification of a Salmonella BS-20062 virulence gene;

FIG. 2 is a plaque morphology of bacteriophage RDP-SA-20051 of the present invention;

FIG. 3 is an electron micrograph of bacteriophage RDP-SA-20051 according to the present invention;

FIG. 4 is a graph of the effect of bacteriophage RDP-SA-20051 of the present invention on the growth curve of Salmonella BS-20062;

FIG. 5 is a schematic diagram showing the acid-base resistance of the bacteriophage RDP-SA-20051 of the present invention;

FIG. 6 is a schematic thermal stability diagram of the bacteriophage RDP-SA-20051 of the present invention.

Detailed Description

The invention is further illustrated by the following figures and examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.

The invention separates salmonella BS-20062 from the body of a diseased and dead chicken in a certain farm in Shandong province, determines pathogenicity and drug resistance of the BS-20062 through experiments, separates a virulent salmonella phage from the environment by taking the BS-20062 as a host, and determines the genotype of RDP-SA-20051 through a whole genome sequence.

The salmonella bacteriophage RDP-SA-20051 provided by the invention has good advantages in treating salmonella infection diseases, is wide in cracking spectrum, does not cause damage to probiotics, is high in potency and is good in prevention and treatment effect.

Example 1 isolation and identification of pathogenic bacteria and phages

1. Isolation and identification of pathogenic Salmonella BS-20062

Sampling from viscera of a dead chicken, streaking on an SS culture medium by adopting an aseptic operation method, culturing at 37 ℃ for 18-24h, and then displaying a white and regular colony with a black center edge on an SS agar plate, wherein the diameter of the colony is about 2-3 mm; and (3) selecting a typical colony, streaking the typical colony on an LB agar plate, culturing the typical colony at 37 ℃ for 10-24h, then performing diffusion growth on the LB agar plate, selecting the typical colony, further streaking and purifying for 3 times, then selecting a single colony, inoculating the single colony into 5mL of LB liquid culture medium, and performing shaking culture at 37 ℃ and 200rpm for 8h to obtain a uniform and turbid bacterial suspension. Then identified as salmonella through 16sRNA molecular identification, and one strain is named as BS-20062.

2. Drug resistance test of salmonella BS-20062

The separated salmonella is subjected to in vitro drug sensitivity test of 10 antibiotics of 6 antibiotics, and the results of the selected drugs and drug resistance are shown in table 1. According to the CLSI standard, the method is carried out by using a round paper diffusion method.

Note: r, drug resistance; i, the intermediary degree; and S, sensitivity.

As can be seen from the experimental results, the Salmonella BS-20062 is resistant to most antibiotics.

3. Salmonella virulence gene identification

According to the research results of salmonella virulence factors at home and abroad, 10 pairs of genes influencing salmonella virulence are searched in GenBank, and gene primers are designed and synthesized: fimA (9), pipC (1), stn (2), invJ (3), sseL (5), spvC (4), spvB (6), spvD (7), spvR (8) and ssaB (10), extracting a DNA template of salmonella BS-20062, then carrying out PCR amplification, and carrying out 1% agarose gel electrophoresis detection after the amplification is finished. The PCR amplification detection result of the related virulence genes is shown in FIG. 1. The bacterium contains 9 kinds of virulence genes, i.e., fimA, pipC, stn, invJ, spvC, spvB, spvD, spvR and ssaB. The virulence gene detection concludes that the pathogenic bacteria has extremely high morbidity, and the drug-sensitive experiment discovers that the drug resistance of the bacteria is extremely high.

4. Separation and identification of salmonella phage

(1) Sampling: the sample for the test is collected from sewage around a diseased farm in 2020 and is used as a water sample for phage separation.

10 ml of sewage is taken, centrifuged for 5 minutes at 10000rpm, and after larger impurities and most of bacteria are removed, the sewage is filtered and sterilized by a 0.22 micron microporous filter membrane. 3 ml of the filtrate and 3 ml of the host bacterium suspension were added together to 20 ml of the 2216E liquid medium after autoclaving, and then placed in an incubator at 37 ℃ for overnight culture. After the culture, 5ml of the culture solution is taken, centrifuged for 5 minutes at 10000rpm, and then filtered and sterilized by a 0.22 micron microporous filter, and the filtrate is the stock solution which is supposed to contain the phage. Then, a double-plate method is adopted to identify whether the phage exists, if the phage exists, otherwise, new screening is needed.

(2) Purification of bacteriophages

The plaques isolated initially are often of inconsistent size and morphology and require further purification. Picking single independent, uniform-shaped, clear and transparent plaques on a double-layer plate with plaques, soaking the single independent, uniform-shaped and clear and transparent plaques in 1ml of physiological saline for 10 minutes, taking clear liquid, filtering the clear liquid by using a 0.22 micron microporous filter, properly diluting filtrate (single plaques exist when the double-layer plate is laid), laying the filtrate on the double-layer plate together with host bacterium suspension, repeating the step for 3-4 times, and obtaining purified phage when the size, the shape and the definition of each plaque on the double-layer plate are consistent.

(3) Determination of phage titer

Spreading the purified phage on a double-layer plate, taking a single plaque, adding the plaque into an LB liquid culture medium, simultaneously adding a host bacterium suspension, proliferating the phage, centrifuging the proliferation solution at 10000rpm for 5 minutes, filtering by using a 0.22 micron microporous filter, and diluting the filtrate by 10 times in a gradient manner to 10 times⁸Taking 0.1 ml of phage diluent and 0.1 ml of host bacterial suspension of the last 3 dilutions, spreading a double-layer plate, and determining the titer of the phage.

Phage titer (pfu/mL) is plaque number × dilution factor/0.1

The results are shown in FIG. 2, which shows that the plaques have consistent morphology, size and definition and have typical lytic phage characteristics. The titer of the bacteriophage can reach 10¹³pfu/mL or more.

Example 2 morphological Observation of phages

Applying a phosphotungstic acid negative dyeing method: in the paraffin wax sheetPhage liquid (titer 10)¹⁰pfu/m L)100 mu L, placing the surface of the copper net with the film on a phage droplet, taking down after 10min, naturally drying in the air for 2-3min, then dropping a 2% phosphotungstic acid (PTA) aqueous solution on the copper net for dyeing, taking down after 10min, drying in the air for 10-15 min, observing by using an electron microscope, and selecting a clear phage image for photographing. As can be seen from the electron micrograph in FIG. 3, the phage RDP-SA-20051 is a long-tailed phage with a regular hexagon head, a head diameter of about 52nm and a tail length of 110 nm.

Example 3 phage RDP-SA-20051 genome sequencing

After enrichment culture of a single phage, centrifuging for 15 minutes at 8000g under the condition of 4 ℃, adding 10% PEG8000 and 0.5M NaCl, standing overnight, adding equal amount of chloroform, mixing uniformly, centrifuging for 10 minutes at 5000g after standing and layering, removing a chloroform layer and a PEG layer, adding restriction endonuclease for digestion treatment, suspending the phage under the condition of gradient density cesium chloride, dialyzing for 3 times by TM buffer solution at the later stage, each time for 30 minutes, and finally sending the dialyzed phage to a Huamao gene biological sequencing company for whole genome sequencing.

Example 4 optimal multiplicity of infection experiment

According to a multiplicity of infection of 1: 100. 1: 10. 1:1, 10: 1. 100, and (2) a step of: 1, adding the phage multiplication solution and the host into an LB liquid culture medium, and ensuring that the total volume of a culture system is the same; performing shake culture at 37 deg.C and 200rpm for 6h, centrifuging at 12000r/min at normal temperature for 5min, collecting supernatant, spreading on double plates, and measuring titer; the results are shown in Table 1.

TABLE 1 phage optimal multiplicity of infection test

As can be seen from the data in Table 1, when the infection complex number is 1:1, the proliferation fluid is relatively clear and the titer is the highest after 6h of culture, which indicates that the optimal infection complex number of the salmonella phage RDP-SA-20051 of the present invention is 1: 1.

Example 5 Effect of bacteriophage RDP-SA-20051 on Salmonella BS-20062 growth curves

Inoculating the host bacterial suspension cultured for 6h into 100mL LB liquid culture medium in a proportion of 3%, adding phage multiplication liquid according to the optimal multiplicity of infection, and simultaneously taking the host bacterial suspension without phage as a control to make 3 groups of parallel controls, and taking the average value; the results are shown in FIG. 4.

As can be seen from FIG. 4, the host without phage was continuously proliferated with time, and its OD600 was gradually increased, while the host with phage was added, with the exponential increase of the number of phage, within 5h, the phage lysed host, and its OD600 was not significantly increased and slightly decreased, but proliferated after 6h for the host bacteria tolerant to phage, but at 10 h, the host bacteria was suppressed again, and finally, with time, its OD600 was gradually increased, and finally, leveled off.

The Salmonella phage RDP-SA-20051 is added into the suspension of the host bacteria BS-20062, the OD600 is almost unchanged within 5h, a certain cracking effect on the host bacteria is achieved, compared with a control group, the host OD600 without the added phage is obviously increased, and the phage has stronger cracking capability on the host and can continuously crack the host bacteria. After the continuous action for 6 hours, the mixed bacterial liquid turns turbid, and a flora replaced by the mutant tolerant bacteria alternates, but the phage generates an antagonistic strategy for the phenomenon, so that the OD600 is reduced again, the increase of the bacterial number is continuously suppressed, and the specific coping mechanism is not clear. In conclusion, the bacteriophage has a good inhibitory effect on the pathogenic bacteria.

EXAMPLE 6 determination of acid-base stability

Adjusting pH of normal saline with dilute hydrochloric acid and dilute NaOH solution to obtain buffer solution with pH of 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, and diluting phage into 1 × 10 with the prepared buffer solution¹⁰pfu/mL, carrying out water bath on the diluent for 1h at 37 ℃, diluting the diluent by 10 times of physiological saline, paving a double-plate, carrying out inverted culture at 37 ℃ for 4-6h, and counting; the results are shown in FIG. 5.

As can be seen from FIG. 5, the phage was allowed to act for 6 hours at a pH in the range of 5 to 9, and its titer was maintained at 1X 10¹⁰Above pfu/mL, at pH 7, the potency is highest; the optimal growth of the phage RDP-SA-20051 in a neutral environment is shown; at pH 12 and pH 2, the phage still survived, indicating that the phage has a wide range of acid-base tolerance;

EXAMPLE 7 measurement of thermal stability

Subpackaging the phage stock solution into EP tubes, incubating at 30 deg.C, 40 deg.C, 50 deg.C, 60 deg.C, 70 deg.C, 80 deg.C and 90 deg.C for 30min, diluting with 10 times of normal saline, spreading double plates, and counting; the results are shown in FIG. 6.

As can be seen from FIG. 6, the phage RDP-SA-20051 has stable titer at 1.0X 10 within 30-60 deg.C⁹pfu/mL or more; acting at 80 deg.C for 30min, and keeping the potency at 1.0 × 10⁸pfu/mL or more, and has good high temperature resistance.

EXAMPLE 8 cracking Rate experiments

Under aseptic conditions, 1mL of sample and 1mL of host bacterium solution (1X 10)⁵CFU/mL), incubating for 15 minutes at 37 ℃, mixing uniformly, and diluting to 10 with physiological saline^-1-10^-3Each gradient was applied in an amount of 100. mu.L, spread on LB agar plates, incubated at 37 ℃ for 24 hours, and each gradient was repeated twice. Simultaneously taking 1mL of physiological saline and 1mL of host bacterium liquid (1X 10)⁵CFU/mL) as a blank, repeat the above steps. Plates with 30-300 colonies were picked for enumeration. The test was repeated 3 times and the average was taken. The phage lysis rate was (1-number of treated colonies/number of control colonies) × 100%.

The result shows that the cracking rate of RDP-SA-20051 reaches 98.9%, and the composite has good cracking effect on the host, and is suitable for being used in the culture process.

It should be noted that the specific embodiments are merely representative examples of the present invention, and it is obvious that the technical solution of the present invention is not limited to the above-mentioned examples, and many variations are possible. Those skilled in the art, having the benefit of this disclosure and the benefit of this written description, will appreciate that other embodiments can be devised which do not depart from the specific details disclosed herein.

Sequence listing

<110> Ray union (Qingdao) bioengineering Co., Ltd

<120> Salmonella furiosa phage and application thereof

<160> 1

<170> SIPOSequenceListing 1.0

<210> 1

<211> 43483

<212> DNA

<213> 2 Ambystoma laterale x Ambystoma jeffersonianum

<400> 1

cttaatagac gctcaggggg tgtctgatgc gctatgatgg tcatacgcat ttaagagagg 60

attgcatgaa actcaaatta aaacagcctg acgccgaagt agtggctgcc gcgcacgaag 120

aagcggttaa cgcaaaccgt cgccgtaaac gtccgcgtgg taaacaaagc ctgtatcagt 180

catccagaca ttccgccgaa ctatgggacc cggactattg cgacgaatta atccggttct 240

tcgaccgcac gtcatgggaa ctcgtaccca cgtctaaagg cgacgaacgc ccgcttatcc 300

aggataaacc gccatcactg gcccgtttcg cactacacat aggcgtaacc gttccgatta 360

tcaagctgtg gctgcgagag gttcctgcct tcgctgaagc atacgaaacg gcgcaggccc 420

tggaagaggc gtatttcact gagaccggtg ctgccgggat atctgctacg ttcgccgcag 480

caaaacttgg gcttaataaa actgttgtgg aagaagcgcg cgacgaacca attagcgaag 540

taactattaa ggtggtgtcc ggtgaacgtt gatatcacag ctacgggacc gcagggcgcg 600

ttccttaatc tgcattgtaa gttcccggcc ttcgtcgctg gcttcggcac aggtaaatcg 660

gaagtcatgt gcaactccgc cctgttagac agcatggaag gtggtagcga ttcccttatc 720

gccatgtacg aaccgacgta tgacctggtt cgccttatcc tcgccccgcg tatggaagag 780

aagttgtctg attggggtat tcgttacaag tacaataaat cagacaacat aatctataca 840

tcatccgggc aattcgggga ttttgtcctg cgtacattgg ataacccggc gcgaatcgtc 900

ggttacgaat cttttcgcgc aaaaatagac gaattggaca cgttaaataa agaccacgcc 960

gagcacgcct ggaacaaagt tatcgcccgt aaccgtcagt tgccgcgtac atatcgtccg 1020

attaccccga agcctgctaa tacagtttcg gtatttacga caccagaagg cttccgtttt 1080

gtgcacgaca gatgggctgt aaaaaagaaa ccaggctatg aaatgattca ggcctcgaca 1140

acatccaatc cctttctacc ggaagattat gtgcagtcgt tgcgggatac gtatccaggc 1200

cagttaattg acgcctacat cgacggcgaa tttgtcaacc tgacatccgg cagcgtgtat 1260

tatgcttacg accgacgtaa gaacagcagc cgggagacta tacaacctgg ggaaacgctg 1320

tatatcggtc aggacttcaa cgtcgggcat atggctagca ccgtatacgt tcagcgtgag 1380

tatgtctggc acgcggtagc cgagctggtg gatatgttcg acaccccgga tgtggtcagg 1440

gagattaccg aacgatggaa gcgtaacggg catcacattg ttatgtatcc ggacgccagt 1500

ggtaagaacc gtaaatcgac tgatgcaagt acctcggata ttgcccaatt acagaacgcg 1560

ggtttcgaga tacgtgcaaa atcggttaac cctgccgtta aagaccgtgt agcatcggtg 1620

aataaagcgc tggagtctgg tagattaatg gtcaacgagc aaacctgtcc ggttactgct 1680

cgctgtcttg agcaacaggc atacgataaa aacggcatac cggataagac aagcggcaac 1740

gaccaccaga acgatgcgac aggataccct atcgcctacg aaatgccgtt ggttaaacct 1800

gtttcccata tcccggttac ttttgcactt taagaggatt attaaatgtt aacagcaaac 1860

ggccagggtt ctggcgtaaa aaccaagcac cgtgaatggc tgcactacgc gcctaaatgg 1920

cagaaggtgc gccacgcgct tgctggggac ctggttggct acttacgcaa cgtcggcctt 1980

aatgaaccag ataaagcata cggcgaagcg cgtcaggcag aatacgaggc gggcggcatc 2040

atatacaact tcacccggcg cacgctgtca ggaatggtag gtagcgtcat gcgcaaagaa 2100

ccggaaatca atatcccgaa ggagttggaa tatctgctta aaaatgcaga tgggtccggt 2160

gtaggcctaa tacagcatgc gcaggacacg ctcatggaga ttgactcagt aggtcgtggt 2220

ggtcttcttg ttgacgcccc ggaaacaggc gcagctacgg ctgccgaaca gaacgcgggc 2280

ttgcttaacc ctacaatcgc tttttacacg actgagaaca tcgttaactg gcgactcacg 2340

cgcgtaggtt ctgtaaaccg ggtaactatg gttgtgctgc gcgagacatg ggagtacaac 2400

gaaccgggaa acgagttcga aacgaaatac ggcgaacagt atcgcgttct ggacatcgat 2460

agcgacggca actaccgtca acgtcttttt cgtttcgatg ccgaaggtgg tgcgcaggaa 2520

gacgtcgtgg agatttaccc ggacttaggg gaatcattac gaggggtaat tccgtttacc 2580

tttattgggg cgaccaataa cgacgccact atcgacgacg ctccgctact accactcgcg 2640

gaacttaaca tcgggcacta caggaacagc gcggacaacg aagaatccag cttcgttgtt 2700

gggcagccga ccctgtttat ctacccaggc gagaacttaa cacctcaagc attcaaggaa 2760

gcgaacccta acggtatcaa atttggtagt cggcgtgggc ataacctggg gtacggaggt 2820

agcgcacagc ttatccaggc aggcgagaat aacctggccc gccagaatat gctggacaaa 2880

gagcagcagg ctatccagat tggtgcgcag cttatcaccc cgactcagca aatcaccgca 2940

caatctgccc gaatccagcg cggtgccgat acatccgtca tggcgacaat tgcgcgtaac 3000

gtaagccagg catatacaga tgctttacgt tgggtggcgg taatgttggg taaacccgaa 3060

gataccgagg ttgaattccg ccttaatatg gacttcttcc tggaacctat gacagctcaa 3120

gacagggctg cgtggatggc agacattaat gccggattac tgcccgccac tgcttattac 3180

gctgcgttgc gtaaggcggg ggtgactgac tggaccgacg aggatattct gaacgctatt 3240

gaagatgcac ctttgccgtc gggtgctgtt actcaggtag cgggggagat tccgcaagcg 3300

gcacaacaac aacaacagga gtaaagaaaa aggccccatt acggggcctt agttttagcg 3360

gatattagcc gcgcatttac gcacaacccg cggtatcgtc tctttcagct catcccatga 3420

gttgactgct tccatatcca acaacgtcac catagccttt tgaatatcac tccgtaattg 3480

attagttttg gcgtcctgag agtcccaagt atcaaaaccg gttacttcac ggatatctat 3540

agactctttt atcgcattcg ccacatgtat gaagtgaccg atacgcccaa tcctaccctc 3600

cgtacgctca atcatccggc taacggcggc attaagttct acaaaactaa cagcaccaat 3660

gttacggagt tctacctgcc gttgagttaa gaaacggtct ataacgagga aatggaaata 3720

agtgctgtat tgctcagcaa tatataccgc caaatgaaga cttaaccacg ttttcccgtt 3780

cctccctctc gttgttttca acggaacaac gccagtctcc tcttcacaaa cacgcatgaa 3840

gtctctgcag tcgtccgttt tcagtatctg cgcaagattt cttggttgaa gaccattatc 3900

aaatctccat tggttcattt gaatggttag tgttgacgca tcaatatact catcgctggt 3960

gaactcaatt tcaccgtagg ggaactctaa agtctgcgct ttcatatctt tttcctcagt 4020

taattgcctc acttaaatta tattcctaat ttctttgttt atgcaaacat taatacgcaa 4080

aattatagca aattagactt ttttaggtgt ttgtgtagga ataacctatg ccatgctaag 4140

atgcatcaac aacgcaaaca ggagggattt atgccactac ttacatcatt aatcagccat 4200

caaatatggc tgcaacgcaa cgccagtagc gaagtaaaag acctggcgcc attcatccag 4260

cagatgcgcg atgaggttaa gcggcaggtg ttgttgtttg gtgatgacag cagaacggcg 4320

gcgagactta ccaccatgct gcgggaactt gagcaagcgc tgaacgggat tacgtctggg 4380

tggtatgaga agctactggc cgatgcccgt gagctttcag actatgaagt taactggaac 4440

gtaaaaaccc tgtcgaccaa cgttaacgct aatttcgtaa caccggccgc cgaacaagta 4500

tgggccgcca ctactttcgc accacttgaa ctaagcgaaa agccggttga ttttgtttcg 4560

ttaatgggcg gatggcggca agccgaggtg aatcgcttgg ttatgggcgt taagtctgga 4620

ttcgtacagg gtatgaccac acggcggata gttaagaacg ttgtcgggcc cggcggactg 4680

gctgatatct ccgagcgtaa cgccgctacc gttatccgta ccgccctggc gcacgtatcc 4740

aacgaagcgc ggcaacaggt ttacgctcag aacgacgaca tcattacaaa atacgagtgg 4800

gtatcaaccc ttgattcccg tacttcggct gtttgtcgtt cgcgagattc tatgcagtac 4860

gaaataggca aaggtccgct accaccagcg catccgaact gtcggtcgag tacagcaccg 4920

gtaatcagtt ccgaattcga cttcctggat aagggtgcaa aacgagcggc gaggggtgca 4980

gaaggaggcc agcaggtaag cgcagacacc acctattacg agttccttaa acaacaaccg 5040

gcgtggttcc aggacgaagc gctcggtcct gtcaggggta agatttttcg taatagtggg 5100

ataaccccgg aagaatttcg tgtaatatcg gtagacggat tcgggcgtcc gttaactctt 5160

aaagagatgg cggaactcga taggcgtgtt gccgattatc tgaaagagga atagagatgg 5220

gctttttcaa agttaaagat gtgccttcac gacgtgtagt tcagtactcc cgtgtgtctg 5280

gtgcgggtga aggtgtcgtg tatatcaaag atgaatcggt tctcggtgaa tcggtggacg 5340

aaatgccgtt tgcagataag acaggactga ccacaatctc ggacggcgtc ctgtacgaag 5400

tgccgtatct ggacggagca ggtgatgtgt acttcgatac acagccagca gatgtagaat 5460

tgaaagacgg ttccgcaaaa ttaaccgttg tcgtgaaagg tggcaaagcg ccctacgatt 5520

tgcaatggtt taaaaatggt aaagaggtga tcaacgtccc gtacgttgag ggtgaactaa 5580

ctgttaaaga ccccggagaa tatttcgtca gggcggtaga tgctgatggg gtatcggtgg 5640

taagtaaagc ggctaaggtc tcagaacctg agtaacgaaa ggccccgtca aggggcctta 5700

attttacaca ccattattcc agttattcct tacaaatgta aattcaggaa tactttattc 5760

aaaaagtaga cttctattat tttttatgtt acgctaccta cgctttcagc gtaccgcggt 5820

gcggacgcgt gaagcgggcg ccacagcgcc ctagcgtaac cgcgaacgca ttcggaaggg 5880

ccgagctata ttgctttcag tatttatcta agattcgccg tatgatgttt acagatatac 5940

gagtttaacg cccggcttac gccgggctta gcgtataaag aaaggggata ccatgaattg 6000

gaaagtaacc gcagtaatca cggcagccgg catcctttcc ttgtggttat acggccagta 6060

caactacaga agcgggtggg tagaaggccg agagaatctt gtttcacagc aacaaaagaa 6120

ggcacaagct gggctgacga agaaaacaca acggcagcag cagaatgata ctaaggccgc 6180

ctccgccgaa tcggaaggca aagaaaattc ggaggctatc acccatgaag tcatcaaata 6240

tgttacccgt cctggccgta ctgtctgtga gtttcctcct gaacgggtgt caatcaaacg 6300

acgcgccgcc gagaatgcta attccatccc cggatatgac actaatgcaa ccgccgtgca 6360

agcttattcc accgagcagt gacgctgatg aggacctggc cgtcgatgtg cagaacgccg 6420

agtgcacacg gcagttacgt ctgaaagtgt tccggttgca ggagtacata aggaatattc 6480

tggaatagtt gccttagtaa gtggaataat ttattcttgt tacagaaaca ccgggtggcc 6540

cggtgtccta aagtccaggg gacatattga ctatgaatcg ttttttacgt tacccgcttc 6600

atgaagaagc cggggtagaa gataaaccgg gtgcaggtga cgcaccaaaa atgtacaccg 6660

ccgaagaagt gcaggccttg attgagaaag aagtggccgg gctcaaggca aatcaggaag 6720

cgctccttag cgagaaaaag gaagccgcgc gccgggctaa agaagccgaa gaagaacggc 6780

aacgcgccca ccaggaagca ctgaaggctg ccggtaaaat ggacgaattt gaaaagacga 6840

ttcgtagtca gtatgaaccc gtactgaaag agaaagaaga acgctatgct tctttagccg 6900

cgcgaattct tggtagtgaa cgtaaggctg ttttaggttc tttcgctggt gattttatca 6960

ccccagaagc ggtagaaatc ctggcgccgt tcgttaagac cgagtttgaa ggcgaagacg 7020

tagtgactaa attcatgggt gcggacggca atgttgttac taccgaccct gagcagttcc 7080

gcaaatacct gcgcgaacat aaagcttttt cgcatttgat taaagcaaat gcagcttccg 7140

gtggcggggc ttccgggaat aaaggcggcg gggccgcacc agcgtttaaa gatatgagtg 7200

aaagtgagcg gttggctctc tataaatcta accctgccga atttgaacgg caacttaaag 7260

ccctgaggaa ataaataatg gcaatcacca ctattggtaa tatcgtaact ggcaatatcc 7320

ctgtactggc gtcctatatg acggaagacc cggtagagaa aaccgcgttt ttcaactccg 7380

gaattttgac tccaactccg tatgctgccg agattgcccg aggcccgtct aacatcgcta 7440

acctgccgtt ctggaaagct atcgatactt ctatcgagcc taactactcg aatgacgtat 7500

accaggacat cgcgaccccg cgcgctattc agaccggtga aatgatggcc cgcgttgcat 7560

atctgaacga aggtttcggc caggcagacc tgactgtaga acttaccagc cagaatccat 7620

tgcagtctgt agcctctcgc ctggataact tctggcagcg tcaggctcag cgtcgtctga 7680

ttgctaccgc cctcggtctg tacaacgaca acgtatccgc tactgatgca tatcacgagc 7740

agaacgacat ggtggtcgat gtttctgcaa cttcaggctt cgacgcaggt gcattcatcg 7800

atgctaccca gactatgggc gatgcattga tgggcaacgg cggtgaggtt ctcggtgcta 7860

tcgcaatgca cagcttcgta tacgcacagg cccgtaaagc tcagcttatc gacttcatcc 7920

gtgacgctga gaacaacacc atgttcgcca cctaccaggg ctaccgtgtt atcgtcgatg 7980

acagcatgac cgtagtaggc cagggtgcgc agcgcaagtt catctccgtc atcttcggcc 8040

agggtgctat cggctatggc gaaggcaatc ccgagatgcc gttggaatac gagcgtgaag 8100

catctcgtgc taatggtggt ggtgttgaaa ccctgtggac gcgtaaaacg tggttgctgc 8160

atccgtttgg ttacagcttc accagcgctg taatcaccgg caacggtacc gagactatcg 8220

cacgttccgc tagctggcag gacctggcta acgccgccaa ctggaaccgt gtagttgacc 8280

gtaagcatgt gccaattgcg ttcctggtaa ctggtgtagg tgcttaatcg tagggtatac 8340

tcttgaggga cttcggtccc tcttttcatt tatacggaga ctacataatg tcaaagacag 8400

gtaaagggct gccccgcagc cttgttaacg cggaattcga tattcccgcg gcgaccacaa 8460

cggcaatcgg aggggttaaa aagtccgcta cggtagcggc accaccggcg attagcgcgg 8520

gtagcggtgc agccgccgcc gccgccgctg cacctacgaa agaagagttc gacgcactgg 8580

tcaccaacta caataagttg cgtacggatg tgacttcatt gcacaccgcc gttactaatc 8640

tgttaaccgc ccttaaaaac gctggtactg tatcctgagg aggataaaaa tggttgatgt 8700

aattaaacgt cgtattgttg gtgtatctga tgatagtccg caggacgggc aggtagagat 8760

tgatatggaa aacgtgacgc cgttgcgttt ctctaccggt ctcaatgata ctacctcggt 8820

aacatctggg caggctatca ccctgacggt ggtacttgcc gacggaatgg accctaaaac 8880

cgttcaatgg tataaggata ataacgctat cgctggtgca accgctttga cttacactaa 8940

ggccaactcc gcagcggcgg attccggtac ttacaaagtc gtagcgcacg acggttacgg 9000

taatatcatt tcagatagta cggtagttac cgtaagttaa atacacgcgg cctccgggcc 9060

gctttaagga ttagacatga cagataatta cgtagtacgg gagcaataca agggtgtggt 9120

cgaggttgac gggcagttag tcccgatgcg cgaagaagcg aacccggaag cattaatcga 9180

aactcagcca gtggtcgaag aaccgcatta caacggtggc ggagagccta agcagcgtcg 9240

tcgccgcaaa agcgtagagg aataatttat gccgcttatc gtggaaaccg gtcaaggcat 9300

cccgaatgct gactcttacg tcagcctgga agacgggcgc gcactggccg ctaagtatgg 9360

tctcgaacta ccggaagatg acaccgcggc ggaggccgct cttcgtaatg gcgcggtgta 9420

cgtcggcctt tttgagtctc agatgtgcgg acgtcgagta tccgctagcc aggcgctggc 9480

gttcccgaga accggtatta ctctacacgg gttccctcaa ccgtccaacg ttatccctcc 9540

gttaattgtt caggctcagg taatggctgc cgtagagtat ggcgcgggta ccgacgttcg 9600

agggtctaca gacgggcgag aggttcagac cgaacgagtt gagggtgcgg taaccgtgtc 9660

ctactttaaa aatggctact caggcagcac agtaagcatc acggcagccg atgatgcgct 9720

acgtcctctt ttatgcggta gcaataacgc ctactccttt aatgttttca ggggttaatc 9780

atggctaaga ctaaatcaga aatattttcc cttatcggtg ccaacttccc tgataaccag 9840

tctggactaa ttacacctga aaagttgcgc gaagttacca ctcagatggc ggattctacg 9900

ctgtacggcg ttaaggaagt agaggtactt cgcgcgtcgt ctacggatat tcaggcgcca 9960

actaccacag gtacagcgtt aaccgtatcc tttggtgggg cgcagaaaac aagcgctgac 10020

cctgtgatga ttaatgcatc cggggtagtt acgttcaacg ccgctggtaa ctacgctatc 10080

cgtgttaaat tgcaggctgg gcgcaccggg gcgagcggaa catccatcct tctgccgcgt 10140

gttctgctcg gcggcgccca attcggttca ccagccgcta ctaaactggc aagcgcagac 10200

gccacgattc ctattgaatc gcgtgtggta gttaacgccg tcgctgggca aaacttcgcg 10260

gtagagatta tgcgagacgc ggccgggtct aactttggtg gattgtaccc tcaaacagca 10320

acggttactt cctggggcgt agccccatcc gcattgctgg tcatctcaag actggaggcc 10380

gcataatgag caccgctttc agtaaaagga tgcaaggtgt aggaactcgc ctgttaacaa 10440

aatacggcag taccgtaatc ttgattcaga aaggtcaaaa aacatgggat cctgttttag 10500

gagagtacgt ttggggggcg gacacgacgg tacctcttaa atcagttccg gtacctgtta 10560

acgcggggct ggtaaacgga actaccattc aggcaggaga tatggtggtt aaggccgatt 10620

acagcgtaat tcctaagatg gatgataaag tacaattcaa tggtgaacag tggtctgtgg 10680

tcaccatcga gaagaagatg gttaacgatg acgttgtagc atactttatc caggtgagaa 10740

aatgagtttt gcgcttgatg tgtccaagtt cgtggaaaag gctaagaaga atcctgaaaa 10800

ggtaattcgt caggtatcta tcaagttgtt ttctgcgatt atcaaagcga gtcctgtaga 10860

tacaggtcgc tttcgtatga actggatggc gtccggcggt actcccgccg acggaactac 10920

ggacgctacc gataaagctg gcaccacggc gaccagtaat gccgctaatt tcgtgctaaa 10980

tgccgctgac tggcacacgt ttactctaac taacaatttg ccgtatgcgc agcgccttga 11040

gtatggttgg tcgcaacagg cgcctcaagg cttcgttagg gttaatgtca gcaggttcca 11100

gcaattatta aatgaagaag cctctaaggt gaaataatgg caacatattt cgaggattta 11160

acaaaagcat ttgacacggc actggtagca ttcggcgtta acaacggtat aaaggttgcg 11220

ttggagaaca tcgacgcacc gacgtctacg gatgtaccat atttggcgag ttatatgttg 11280

ttggccgata cggaacaagc tgacctgttc tggaccgagc aacgggcagg tatttatcag 11340

gtggacatta acgttgggtc taccttgggt agcgcccctc tcaaccggtt agctgataag 11400

ctaaacgccg ccttcgccgc cggtaactgt tttagtcgta acgaaatctg tgctgaggta 11460

caatcagtaa gcctcggtcc tcttattgtt gagaatggat gggcgaagag gcctctctca 11520

attaatttca tagcatttac agcgaggatt agataatggc gttacaacca tataaaggcg 11580

cgatgaccgc gcagttttac gttcttgaga cgacgccggg ggtaacaccc actaacccgg 11640

tatggcaacc actgcgcaac actgggggta ttcccgccgt aacccgcgac gccctcatct 11700

ctaatgaact ggacggcagc cgtgaaacat catctatccg caccggtaac cgtcaggtaa 11760

ctggtgaata tgctattgaa cttagcgcgg aaagccagga tgagttgctg gccggcgcaa 11820

tgaccagttc ctgggtagca ggttccacta aatcgggaat cagcgttacc gtagacccgg 11880

tggcgaaaac tttcacacgc gcagacggta gctttgtgac agacggcgtt gaagtaggcg 11940

acctggtgca attcgatggt ttatccggaa ataacgacaa agcgttcatg gtcacggctg 12000

tcacagctac agttataacc ggtgcgggta tccagcatac ccttaccgcc gaatccgacg 12060

tccaggccaa tttgcgtatc gcggataaac tggaaactgg taacttgtgt aaaacctatt 12120

caatcctgac atggttgaaa ggtaaatgcg gaaatcctga ttcgtacatc ataactcgtg 12180

gtgttgaatt taccgggttc accatcgaac aagccgttaa cgcgatggtt acaggttcat 12240

tcccgttcat tggcttgaat caggaaatcc tacaagcacc gccgagtggt tcagatttca 12300

cgaccaattt tagcgcccgt ccgtttgcat cggttgacgt atccgcctat gacggcgccg 12360

ctccgcttaa acttatcgac acgttcacca ttactaacga caacagcgcg tccgcacagt 12420

tcgagttagg aaataacagc gtggcatttg tcgaacgtgg ccgcgcggct aacaccttct 12480

cgttggcggg taagctgtac gacatgacgt tattgaataa attcctgaac gaaacgcaaa 12540

tggaggtatc ttctgttctg aacggcccgg acggtgccat gtgtttcacc ttaaaacgcg 12600

cttcgttgac atcagcaact ccggaaatcg gtggtcccga atctgtcacc ctgtctcttg 12660

agggccaggc aaccggcaac cagttccagt cttcaattgt tatccagcgc attaagtacg 12720

cctaagaaaa aaaaaggccc ctaacggggc cttagtttta aatcaagcct tctgctgtta 12780

acttacagat aatccacatt tcaccttctg cgtagaacat agcctgcgag tgcccagttt 12840

ctgtttcacg cataatgccg taacctttat cgatgaacca ttgctggaag acccgaccgc 12900

gttttacgga gcgattgtat acgccaaatt gttcaagttg tttattcatc cacacggcag 12960

attgaccaaa cttctgggcc acctgcgttg cgttgtacaa gttatttctg tcgatgatgc 13020

ggtcgtagac atcagctttt ggtgccattt ccttgttctc aagcgcaagg cgttctttct 13080

cctcccacaa gtccgcagcc aaccttaaag cctcccccat tgtctggggc accggtaagg 13140

atggtttgct cgctcgtgac tccagttcct gccaacggcg gatgatggca gcgcgcattt 13200

tgatgctgta ccctgaaatc agaattaacg tttcctcttt atccaaaaga agcattttat 13260

acgtgcgccc cttgctgtct ttgtaatcgg ctgacttttc agccgattga atctcagaca 13320

acattttgcg gatatcagcc agaacatttt tatgctcctt acctgtcagt tccgcaatct 13380

cacggctgga catggtttgt gcttcactaa cattcattaa ttcgttcatg gtttcatctc 13440

ctcttgttgg tatggttata tttaacttcg aacatgctta tcgttcaaat cagattcttt 13500

atcgtgacga taaaaaaaag gcccctttcg gggccttttt ctcactcatc aatattacta 13560

cggtatttcc gtaacttctc cagactttca attgcttctg ctaagtcggt tcctgtgtcc 13620

ttgtgccccc gcaagcccat gcacaacagt ttcttcaacg cgtgctgtag cgctgggtcg 13680

cggatgtcaa atgccctcag aacgtcgtac acatcacagg taatactatc tccgtcagta 13740

tttgtcattg tgcgattgta tttgttagtc atcccagcat ctccggtgaa atagttaaac 13800

gcgcgacttc gccgtattcg gcactgtaag taataacatt cgcgctgcgg cctgacatcc 13860

atccaccacg tgatgcgtaa gcatctttcg ccgcgagagt gcggtgttgt tcaacaatca 13920

tgttacggct ttccacaatc ttctgatggt gaaggtgacc cacatgggcg taactgtaaa 13980

cgctctctcc gaacgcctta cggaacttgg cgatcataac tggttcgata gcatcgaatc 14040

tcgccttatg cccatggtga aagaaaaggg tcgtcttacc gtgttggacc atcttgtaga 14100

catcagccga tgtatctaca aaaacacgag gttcattatc gtaaagagtg ttaaacattt 14160

cagccaacca aatcatgcct gactcgtcat ggtttccttg cacaattagg agatgaacat 14220

ttttatgctt aacaagcgcc atattaacca cgcgcctgac catacgaatc atgtagcgca 14280

ccagtttctg gtaacgcgtg tccgcgtcca gtacatggcc actggcaggc gtaacggcat 14340

caaggctgtc aaaatgcgca aagtccccta ataggttaat aacacccaca ccggcatcag 14400

gcgccttctg aaacgccgcg tcgaaccatc ttgagaacag gtcttctgca atcttcatat 14460

cccagtcgtc gccactctca tccgcccagg ccaacatacc taaatggaag tcagaaaccg 14520

tgtaaagatt tagcaacttt tcgtttagtc gctttttagg ccgagggacc gaagaaactg 14580

gcgtaatatc ggacttcatt acatctatta cggctcgcat caactcgacc tggcgctcgg 14640

cgtcggcgtc agtcttaacc cactgaagtt tcgtattgcc gaactcatcc accagtgacg 14700

acgtaccctt tattttatac ccatccggca caaggtgact tacgtcacgc ccgtgaccaa 14760

ctcctttctt tgccagacga gctgacctta gctgaacatt acggcgtgac atgttgtact 14820

tctttgcgat ttcggtcggc ccgagaccag cgtttagctc ttgctgcaac tgttcatctg 14880

ttattttacg agtgaccatg cttatttcct gattgtttaa gacaaatctg attctaataa 14940

tgtaagttta atttagccag attatttacc attaacatta gagttacagg cccagatgag 15000

agccccaacc cacggcagaa gaatccaccc cagtaaaaga ttacatacga atattcctaa 15060

tttcgcttta tgattacgta gcaaggctac caggaacggg atgaaataga taaacgcgat 15120

aataccgata aaaacgctca tgttcttaac ctcatattgt ttagttgacg tagagataat 15180

aggacactat taacggtcat gcaagctatt ttgttattct tatttggcgt ctacggtcgc 15240

accggaaaag cgggtggttc ccgcctggcg caactaccaa ccagtaaccg actaaccaaa 15300

gggcattact atgaaactta gcgattttta ctacgaagca gaagccgaga aaggcgcgcg 15360

tatgccgatt cctttaaaag atggtacaga ttcaggggaa tggttgaacg ttgtctctcc 15420

tgaggctgat gttgcggtta aagctatgcg ggccttcacc ctggcgtacc gagcggcggt 15480

aggtaaatta aaaccgcttc gggataaatg cgaagagcaa aaagacttct cagaatacaa 15540

tttaaaaatg gaagacgcgg cgggagacct aaaccggcaa ttggccctcg aattggtgaa 15600

tggctggagt cttgatgacg agttcactaa ggaaaatctt aagactcttc tcacccaata 15660

taagcgcctg gcggaacatg tagtcgtatt ccaccacgaa cagttgcgtc aattgcagga 15720

aaagtagacg cgttgtttca gtttgcccgt tggaacttca taacccgcca cgaaaggcgc 15780

aagtttgaca gtatagccga cgggcacaaa gccgcgctta tcgctatggg ggtaatagaa 15840

gacgcggaag aaacaacgca ggacgccggg cctgaatgcc cccctgaact actaaccact 15900

tttgagaagt atcgtgatgt taaatttacc cgccgcgtcg atgacgacgg cgtaaagcta 15960

tatccgagag agcaacttag ctggtcggat ttagtggcat atagcactat ttcaggtcag 16020

aatataggga tgtttgaatc tgaaattatc atgggcttag acgccatttt tgagggcaga 16080

aacgatggct gatgtagcta gcttagtagt aaaggtaacc gaacaaggcg cgaaggccac 16140

atcagaccgt cttgataacc tctctaaatc cgcaaaagtt gcgggcgccg ctgttaccgg 16200

cctggccgcc gttgtagccg ctacagccta taaggcggct caggaactag tcgagtcaca 16260

acggcaactg gataagatgt cggccagcct gaaaacacta accggaagca cacaaggtgc 16320

aaaacaggcc ctgagtatat tacaggactt cgctcgtgac acaccttacg gccttgaaca 16380

ggcggtagaa gggttccgta aactggtagc tctaggcctc accccatcag aagaagcgct 16440

acgctcttac ggcaatactg catcagcgat gggtaaagac cttaaccaga tgattgaggc 16500

cgtcgcagat gcgagcacct ttgaattcga acgtctgaaa gaattcggca ttaaagccaa 16560

gcagaaccaa agtgatatcg aatttacctt ccaggggacg actactgtag ttaagaaaaa 16620

cgctgccgat attgagcaat atctcctcaa cataggcaac gttaacttcg ccggggcgat 16680

ggctgaccag gctaataccc tgaacggagc tatcgctagt gcggaggact catggtccca 16740

gttgaagatg actctcgcta ctagtctgga tgtagggtcg ctggcggaac ctttacgtta 16800

tattgacgac ctgatacagg agataaacgc tcaggtcgca tccggtgagt tcgtagccga 16860

gatgcggatg tggggtgaca tggcatccga tgttgggggt gcgatagagg cttcattcga 16920

cgcggcgttt gggatggttg cagacgcgtt aaacgctttg aactccgcct ggacttacac 16980

cagtgaaagc attaccggaa gcggagagga gaccgcatct acgatagctg agtcagcggc 17040

ggatgcgctg gacttcattg cccaggaatt tacggcaatg gagcggtttt ttgaagatat 17100

ggttaaaggt gctcaggatg caggacgtct tgtaaaggcc gcattgacgc cgggagagtc 17160

ggtagctgag gccaagaacc ttaacttcca gttagcgctt gctatggata ctcaaagggg 17220

tgtggctgac ctgacacgga aaagtttccg cgaacaagta gaagctcagg aagaccttat 17280

cgcgctgaag cgagcggctt acgacatcga taaagaagcg gccaaggccg agggtcttgg 17340

taagtttaaa gtgtcgggta aagacaacgg ttctgtaggc gattccgcgg acaatgctgc 17400

taagaaatcc gtcgacgcat tcgaacgtca gaagaaagcc gctgaggatt tctattatca 17460

gtcaatccac cttaacgacg acgtattcca gaagatacaa gctaaccaag aagagcaact 17520

tactaagcta caggagttct acagcaaccg tctccttagt gaccagcaat acgaaaccgc 17580

taagacgcag attatgctcg aggcggatac ggcccgccag gctgagttag ataaacgcga 17640

gaaagagcgc ctggaaaaac agttctccgc ggatgcgtat gtcgctcaga tgcgggccct 17700

tgctgaagga gaatttgcgg aattagaccg gcagtacgag gtcaagctac aaaaacttaa 17760

cgactttcat gcgcagggtt tgattgccga agaaacttac caacaaacgt taagcgccat 17820

ggatgaatct tactcccttg accgagcgaa ggcgaccggc gcggctttcg gcaatatggc 17880

tagtaacatc ggggccgcgc taggagaggc ttccacggca tacaaagcat ttgccatcgc 17940

acaggcgaca atcgctacgt acacatcagc ggtcgaagcg tataaatcaa cagcagccat 18000

accggtagtc ggcccgtatc tggcgcctgt tgctgcggct gctgcggtgg cggctggttt 18060

agcaaacgta ggtaagattc gctccgcccg agaacagggt ggtaacctgg ccgcagggca 18120

gatatccact attgcagaac gtggtaaacc agaagtaatc atgcccgcta gcgcctcccg 18180

tgtccggacg gcggagcaga tgcgacagat tatgggtgag agcggcgcta aatccggcgg 18240

ggataatgtt actattgtga acaataccac cggaagaatt gattctgctg caacagaacg 18300

cgacgatgaa ggccggttgc gtattataat cagtgaaacc gtaagttcag cgttgctgga 18360

tagtaacagc gccatttcta aatcacgtcg cgctacacgc ggccaaccag gatattgata 18420

tgagcgatta ccatttcccg gcctctttga ggccgatagt atcgaaaggc tactcgatga 18480

cgcgaggtaa caacgtgtgg cgggtagacc tggccggagg cggagttcgc caggggcgag 18540

acacctattt tgacgtgttt ccgattaacg ttaccctggt cgtatcgccg ttgggacggc 18600

aggcattcct cagcttcatg gagaaagtag acggtggcgc atcctgtttc tggatgaaac 18660

acgaccttgg ccagggtatt gacgattacc aggtaacgtt aacatccacg tggaacgagt 18720

ccacagatga cggaaagaat tgggtaatca ctttcacggc caccgccgag aagtcaccat 18780

tccaggaagc cagcaacgct tgtctgaacc agaacctacc agatttgtac gggtgctatg 18840

gtgattgcct tggcgaattc cttaaaacct acggagtgta tcagactaca ttccctcgaa 18900

tctgggaccc tatgcaatga gtcaggaatc agtagaagcg gcataccggc gtaaactggc 18960

atccaatcca gacggtgaaa tggattttat tactcttgag atataccacc cacttctttc 19020

gaaacggtgg ttactcgtgc gcggggctga cgacttgacc gctactctag agacgggtga 19080

ggttgttaca ttcgagggta cgccgatgga ggccaagaac gccgccaaca ataacgatat 19140

ggaccagacc gcctctttct ctttgccgga tgtgcttaat atactggacg aggaaatgga 19200

ccgcatccct tatgataata aggaattgcc caaattcatc ttccggcgtt acgtgagcac 19260

ggacctgtca tacccatgtg atgggccggt ggtatatgaa ttgcaaacac tcacacaaga 19320

gaaaggagtg ttcacagcgg aaacaggtac acccatgctt aaccaacgag ctaccggaat 19380

cctgatgacg ccggaggaga ttcctttact tcgagggata ctgacatcgt gaatattaac 19440

gattacactg gcctgccgta tgacttccgc cgccgtaatt gttggcacca cgtccgcaac 19500

gtccgggctg acgcggggtt atcaactcca atgttcgatg taacaagccc aacggcaata 19560

gatgcagctt tcgatgacgg ccattcagac cctaaaggtc ttaggcgagt gcttactccg 19620

cagaattttg acgccgttct actcggtgtg aaacatcgag ggcggatagt gtggcacgct 19680

ggggtatatt acgaagggat ggttagccac tgtgagctgg cgtccagaca ggttagactg 19740

gatagtctgg aagaccttaa agatacttat tcggagattg aattttggcg ctagtaattc 19800

actacacacg aaacgaagac ggcacatttg acgttaaacg ttatcgcgat aatccgatga 19860

acttcgtcgt gaaccacgtt cccgacgggg tgccggttcg cgttttcatt gacgaaattg 19920

gagaagataa cgacgtaaca gaagacttcg aagcactgaa agaagacgcg acttttcata 19980

ttgtggaatc cgctggtggt ggcgctatta aaggcgtcat gaagattttt agcgttattc 20040

ttaaaccgtt ggcgaagctc ttgtcgccgt ccgtgaaagg tgcgtcctcg aaccttgcga 20100

actcgcaggc agactcccca aacaacagtc ttactgaccg caacaacaag gcgcgtccgt 20160

acgagcgcag ctacgatatc tgcgggacgg tacaaacaat acccaataac cttatgtcta 20220

cttataaggt gtttaacgcc gctggtaaaa ttgtagagta cggctactac gacgccgggc 20280

gcggctacct cgacatccac cctgaaggta taactgacgg ggacacccgc gtatcagaca 20340

taacaggtat gtcggttgcc gtgtacgcgc cgtatacgtc accaaataat acatccacac 20400

cacaggtcat ggttggcgac ccgatagagc aaggcctgta catcaccgta gaatccaacg 20460

aagtagacgg cgtggttttg aaagcgccca acggtcttgg catttctttc tcttacatgt 20520

ctgggtatcc gtctttgtca ggaaacattg gcactatata cgaccctaca ggcggctcgg 20580

atttttctgg agtactggtg cctagtgaca cgttttcgct ggtgtccgcg tggacaaata 20640

cagacgttga cctgtccggc ggcgggtatc aggtagtcag cgtgtccgaa gggaccgtta 20700

cctttatagt acctggcggt ctcattggta ggtggcaaga aataagaccg ggttcctttt 20760

tccgtggtga cggagaggcc tcgctgcaac cagacaacac gtatgagaaa actttaaccg 20820

attgggtttc aataaaccgt accgaagtag agcgcattgt agccaatatc gccgccgcga 20880

atggtatgta taaagacaac ggcaaatcga aaacactggc gtcggtcacc gctgagatac 20940

agtaccagtt acttgatgaa aatagtgccc cttatgggcc gatatacact gcacaaggaa 21000

ccgtgtccgg gcgaacccca gactacaacg gtgtcactat ttacgccgac ctgccggttg 21060

tgtcgcgggt gcgggtgcgc gccagaaggg tgacagacct cgactttaat ttcgaaggct 21120

cagtggtaga cgaaataacc tacgttaact tgtacgggca aacacgcgat aacactccac 21180

actacggcaa cagaactacc gtacactcaa tgcgcaagca gacaccacgc gccgcggaag 21240

ttaagcaacc acagttacgt atgattgcca ccgaaatggt acacaaatac cttggtaacg 21300

gtgttttcga agatacgatg acccccaata cccaggccgt gcaatctctt atccgcctgg 21360

cgcgtgatcc ggatgtgggg ggtttaaacc tgacggtacg caacatggat aagttacttg 21420

ccgtgcagaa cgaggtcgaa gcgtatttcg gtgacaaaca ggctggagaa ttttgttaca 21480

cgtttgatga ctataaaacc accatgcagg acatagttag tactatagca gacgccatat 21540

tctgcacccc atatcggcgt ggggcggata tccttctcga ttttgagcgc ccccgcatgg 21600

gtcctgagat ggtgttcacc caccgaagca aggccggtac ttctgaaaag tggaccagaa 21660

catttaacga ttctcaggtt tttgacagcc ttaaattctc gtacatagac cctaaaacaa 21720

acgttaaaga aaccataaca atacctgaaa ccgggggcct taaaacggag acttatgact 21780

ctaaaggtat tcgcaactat aagcaggctt tctgggccgc taatcgccgt caccagaaga 21840

acattttaaa gaagatttca gtgtcgttta ccgccacgga agagggtatc tttgctttgc 21900

caaatcgtgc cattagtgtc gttaagggct cacgtatggc tacctacgat ggctacgtaa 21960

ccgcggttaa cggtcttacc gtagaactgt cacaaccagt taagttcacc tcgggagatg 22020

accattcttt gattctcaag ttacgtgacg gcggcgtgca aagtgttaat gtagtccctg 22080

gagcgcacga caggcaggtg attatgacat cagtgccgca agaagccatt tacgtaggta 22140

atagtgcttt gaaaactgaa ttttcattcg gcaacgaagc aaggcataat gctcagatga 22200

ttcttgtttc tacggtagac ccaggagatg acagaacagt caaaataacc gggtttaact 22260

atgacaagga tttctataag ttcgacaacg tgcctccttt cggtcgtgcg ttctccaacg 22320

gattcgataa cggttttaac taagaggata gccatatgtc tagtggttgc ggtgaggtaa 22380

tgtcacttaa tgatttacag gtagctaaaa aacaccagat tttcgaagcc gaggtgatca 22440

ccggcaaaca gggcggtgta gcgggtggcg ccaatatcga ctacgctact aaccaggtaa 22500

ccgggcagac gcagaagacc ctgcctgcgg ttttacgtga tgttggtttc tccccggcgt 22560

ctttcaactt cacaaccggc gggaccctgg gagctgacga cgcggataaa gcggttcttt 22620

ggccgaaaga agatggcggg gacggtaact attacgcatg gcgtggcccc ctgccgaaag 22680

ttatccctgc ggcgtcgaca cctcttacga caggcggcat ttctgattcg gcttgggtag 22740

cttttgggga catcaccttt cgtgcagaag cggataagaa atttaaatac tccgttaagc 22800

tgtccgactt tactacgtta caacaattgg cggatgccgc tgttgatagt gttcttatcg 22860

accgcgatta cactttcagt aataacgaga ccgttaactt cagcgggaag accctgacca 22920

tcgactgtaa agcgaagttt atcggcgacg gaaacctggt atttacgcaa ttaggtaaag 22980

gttccattgt aatagccccc tttatggaga gtgctacaac gccgtgggtg attaaaccgt 23040

ggaccgacga taatcagtgg ataaccgacc ccgcggcaat cgtggccaca cttaaacagt 23100

ctaaaacaga tggataccag ccgacggtaa acgattacgc caagtttcct ggtatagaat 23160

cccttctccc tccggaagct aaagggcaaa gcatatcttc taccctggaa attcgggaat 23220

gtacaggcgt cgaggttcac cgggcgagtg gtcttatggc gtgtttcctg ttccgcggat 23280

gccatttctg taagatggta gacgctgaca acccgagcgg cggaaaagat ggcgtaatta 23340

cctttgaaaa cctgagcggc gattggggca agggtaacta tgttattggc gggcgcacaa 23400

gttacggttc ggtaagtagc gctcaattct tacgaaacaa tggcggtttc gcgcgcgatg 23460

gcggggtcat cgggtttact tcgtatcgtg cgggggaaag tggtgttaag acgtggcaag 23520

gtacggtagg ttctacgacc tctcgtaact acaacctgca attccgggat tcagcagtgc 23580

tataccctgt atgggacggc ttcgatttag gcgcagatac tgacatgaac cccgaagatg 23640

accgcccagg ggatttcccc atttctcagt acccggtaca tatgctccca ttaaaccatt 23700

tgatcgacaa tctactggtt aggggttctt tgggcgtagg tttcggcatg gatgggaaag 23760

gtctgtatgt ctctaacata accgtcgagg attgcgctgg ttctggggct tatattcttg 23820

cccacgaaac agtattcact aatatcgcaa taatcgacac caatactaaa aacttccccg 23880

cgaaccagat atatatctca ggggcctgcc gtgtaaacgg ccttcgtttg gtcggcatac 23940

gctccactag tggacagggg ttgacgatag acgcgcctaa ctccacggta agtggtataa 24000

ccggcttcgt agacccctca aggattaacg tagccaattt gatggaggaa ggtcttggta 24060

actctcgcat aaacagtttc aataatgatt ctgcggcgct tcggttacgg atacataaac 24120

tgtcaaaaac tcttgatagc ggttctgtgt actcccacat taacggcggg ccaggttctg 24180

gttcagcatg gaccgaaatt accgctattg cggggtcctt gcctgatgcc gtgtcattaa 24240

aaataaacag gggcgattat cgtgctgttg agataccggt agcggtgacc gtcctaccag 24300

acaacgctgt cagggataac ggggctatat cactgtatct ggaaggcgat agccttaagg 24360

cgttagttaa gcgggccgat ggaagctata caagattaac tttggcataa atagtaaagg 24420

ccccgtaagg ggccttaatt tatgataaca gaaccacagc agcgataagt aaagcgaccg 24480

cagccaatcc gtagcctatt aaaaagcatt tcgcaccaat tgagtatttc atttagcgcc 24540

cctctctttg tcaacttcct gttgttctag tagccagtca agttgcgcgt tagcagcgtc 24600

tctttgctgc cgtagccgta aaacctcttc ttcgagttcc ctgatacgtt ttatttctcg 24660

ctttaattgc atagctagaa gttgctcgcg ggaattaatg gcgtctttta tcaattcgtt 24720

gcaagcgtcg atatcaaact cattattcat ctcttacctc gtctcttcat ataattaagc 24780

aactcttcct ggacagattt tttctcgtcc gtacgcgcgg caacgacctc atccagcgtg 24840

tctttagcga ctatgtgata caggaacact ggacgctcgt ggccagcctg tttctggcgt 24900

ataggaccta tacgctcgac aacctgcaaa tagtgctcca ggttccagcc ttgcgaaata 24960

aacgccagat gatgcccgcc gtcctgtaaa ttcaaaccat ggcccgccga tgcagggtgc 25020

acgcacaaaa tctcgatttc cccgcggttc caagattcca tctgcttatt acccttagca 25080

cctttcgcaa acgcctgcgc ctgggggaat cgcctaagaa tgcgctcaag ttcgtgcttg 25140

aactgatagg ccaccagcag cggcgcaccc tgcaactcct cgacaatgga ctctaacgca 25200

tcgagtttcg cgtcgtgcac tttctcccag tctttcgttg cttcaccatc cggccccgac 25260

acatacacgg caccggaagc aatctgcaag cacttagccg ttttagcggc ggcgttagcc 25320

gcttcaactt ctccgctctc cagttccgcg aataacttct cctccatatc tatgtatgcc 25380

tgacgcgctt tcttcggcag gtctatttca accggtacaa taatcggcgc gtcacagccg 25440

aaccactcag ccgcgtcaat agtgaggctg atgtctttca tcctctggtg aatctcatta 25500

tctgcgccag ggcgtacacg ataccttcgc gcaatcgcag atttgccttc ctgtaccgag 25560

ttaaaccagc ggtcggtgaa tgcggtgtat gaagaaccga ggcgctcacc agcgtctata 25620

aaccagttct gaccccacaa gtcctttagg ccgttaggtg acggcgtacc ggtcaggttg 25680

atgaaacgct taactttacc gaacgccacc ttactaagcg cctttgcccg cttgctaccg 25740

cctgaacggc tgcggaaaga tttcagtttc gtgctctcat cggccacgat aacagtaaaa 25800

ggccagtcgt ctttgccgta gtaatcaatc agccactcga taacttcgta gttagtgcac 25860

accacattag cgtccgactc cagcgccgct atgcggcgct tctccgaacc ggttgcatcg 25920

atgacacgaa ggcacgggaa accccatttc tcttgttccg ccggccacgt acccgacgcg 25980

acgcgcaacg gggcgaggat taacacgcgg tcgtcatcat taagctgacc attacggaat 26040

aaccggttaa gcacccacat tgtcgagctt gtgttgtgcg ttacggtgaa gtcgcctaat 26100

agaaaccggt ggtcaccgtc gatagtgaag ccgtaataat catctacgcc gaccggggtt 26160

atagattcta ttccaacgtt aagtacgttt ttattaatat tacgcttcgg taaattctgg 26220

tggcggccgc gcacgaaagg cacctcagaa aaatcgcctg ataccgatac gcggaagtaa 26280

tccccccaaa catcagtgtt tgcgcaacgc ttacgagttt tcttcttgta agcggcgaaa 26340

ccaagagaac ggcatagata gcaaaaatca tcagcgagtc gctcgcttac cgaaatccag 26400

tcaaaacctg ctttagacaa atcacaataa ccgtcactat caagaagccc cgccagcaat 26460

tctaaacgtt gttgccggtc gccgcatttg taattgtgcg gtatatgctt attgttcagg 26520

acacccgccg atttcagcgc gtgcgtaaac ccgtgcttct tatgacctgt gttgccgtga 26580

gatatactcc atgtaagtcc ttcttttcgt atttgcatcc cattacgtgc tgcgtacgac 26640

tcaagatacg cccttatttc cttttcgttc tctcctgatg ttatggcccc agacgacgaa 26700

gtaccgtcgc ccagccatag ccccataagg tacggtggca ataaagcctc gtcctgctct 26760

ttccttgtaa aatcgacagg tacccgccaa cccttaagat acccattagg cccggttaca 26820

tatttcggca acttaagcca gtcgcggacg cttatgtcga aaacggtatt atctggccat 26880

gaaccttttg ctatccctgt agtagttcgc agggatagta tgtggctttc gttgacagta 26940

taagactctc cttttcgcgg tttaacctca tacatcatct cccggcctct accgagagac 27000

agtacatttc tcggcgttga atccgggccc ataagaacgt caccgactat aacgtcttct 27060

actttcttgg ttgtgccgtc aaacataata acttcagtcc cgcgtttcag gcacttgccg 27120

ccgcccatac cgcaccagat attgcaacgt gggtgctgta gcataaacga cgtcattatt 27180

ttctgatatt ccctgcgcct aaacttactc atgccttgaa ctccccgaag tatatttctg 27240

cggctctttg gtatacgcgc gacgcttctt ctatagtgtc aaaatagcca aggtggatgt 27300

atttgttatt gacgcctatt ctggcgcgcc acctgccaga gggtatttgg taaaccccta 27360

tcgcgccgct agtgttatgt gatgagcagt ctcggttctg aatctgctgg ctcctggttg 27420

ctggccttat attatttatg gcgttattag ccctgttacg gtctttatgg tcaagctcac 27480

cattatgata ttctccgtag acgaaagccc atgccagatg gtgggctttt attcttacac 27540

cggagactgt aataactatg tatcctttgt cgtttggccc tccggctacc tcccccgcat 27600

ttctcccttt tatgcttact tttcgcgtaa atatacctgt ttctgggtca tagttaagaa 27660

gacgtttcgc ctcctcaata tcgggtcttc tagtgaattt actcatttca tttcaccacc 27720

agcaccaatt ccttacgtcc gaacgctgta acgttaccgg ttacgtcttc gataaccagt 27780

ttaccgttcg actcgacgaa caccgtatca acggcaacag ggctacgtgt cctaacgttg 27840

aaaatcatgt cacccggtac gatgtcacgt gctggtttgc ggtcatattc gtatttcatt 27900

tctcaattcc ttatttgtgt ttggtgtgaa ctaaatataa tagtgttcta ttaatttatc 27960

aacctgtttc ttagacccaa caacaaaaac atttgcacct cgcttacgca tcctctcgtg 28020

ctcccgtaac tggtgcgggt ctggcttcgt gctttcgtct ttcttcacct cgacgaacca 28080

gatgacacca ccgggaagaa caaccaacag gtcaggagca ccggagcgcc cctcatagga 28140

aagtttccga acgaggccac ccagggcctc aaatcgctcc tttgcgtatt tttgcacgcg 28200

cccttcagga gtactcatct atgcacccct cacgtttcgt gtgttcaata ccgcagcgag 28260

gacagattcg acagtcttct tcccggaaat aataaataat cagtttactt agcataccgt 28320

tttaactccg caccttccgc tacaagagga aaaccatcag cccactccgg caatgcacac 28380

attagttttt ccagttcagc taccgtgtaa tctgatgtat ccggagtctc acataccagt 28440

tcatcgtgaa cagaaagaac tattggatac ccaccggctt ccacattaag catcgcatag 28500

gccaataagt cacggcacaa cgcctgaaca atgttttcac aagcctttcc gccgtgtgtg 28560

tacagggtgg tccattgccg tgttaactgg ttctcaccct ggtacttaat tcttacattg 28620

gtgtttatcc gtccgtcttc gtctgtttcc tttgtcacac taacgccgat tcccggatac 28680

gataggatac ggcctgacgg caactccata cacaaccacc agccaggaac cttcctgccc 28740

gacgaatcaa attctacggt acgccatata cggatagccc tttcaccatt ccggcgcaag 28800

tgtgccccag cccaaaattc acgaccagga ttacgaacag cagctaaaat tccgtcttta 28860

aggtcgcgcc agaacgctac tgtttcaggg tgtgactcac gccacatacg cttgatcgcg 28920

tcacatgtgc gccacacctt cttgtccaga atatacgacg gcctgtcatc cttttcaccg 28980

ggatgcggtg gtctcttggc ttcctgaata cgcgcccact catacccacg tgcagtagca 29040

gcccagatgt ggtcggggaa agtcccgtcc attgtttttg ccatctcaat aaggtcaaga 29100

cccaggtttt tagcaaacgt aacgaacgct ccgacacctc cctcgtagcc gaggccaagc 29160

tcacagttat gcgccacaag aaagcccgag tctgaggcta taagaaaacg gttgccgtct 29220

tccacatcaa tcaggtcgta aactggctcg cagtaggtta atctcccgtt caagctcagc 29280

aatgcgggca acggctgcat catactttcg ttgaagctca ggtattcgac gtctgttacg 29340

gcagttttca gaacggctga caaaccgaat gtttccaggt tcgtagttac cgtctgtgtc 29400

gattcggtcc atttcgaggt ttgggtcatc ccagccttcc agagtttgaa catatctaag 29460

aaacgacctt ttatcgaccc tccactggtc aaataccgtg atacccctgg ccccgtagtt 29520

tttatagctc ttgtttccag ggattgttgt tcgtgatatc gccgctgata atcggttgag 29580

taatcgcgtt ctgtgctcgt cgtccggcat tgcgtcttcg tagcagaaat attgtttacg 29640

atattttttt gttgcttcaa gggcgcaacg gttgcattga gtggttttac ctgccctgac 29700

attgtcataa tagacccgcc ccggcatctc tccgcaatca cattggacga gtagctgtct 29760

ttggccttgt cgttttggta actcgcgcag gataaccagg cgtccatacc tatctccagg 29820

gcgcggtcca tcatatattt gtttgaaacg agttgctttg cctgcttcca ggaatgcgtc 29880

aagattttgt ggtcttcggt cattagtacc ccgtccacat ttatcaccgg tttccacccc 29940

ataaggtgcg cgccttttgt attcacccat ttttcaccac tccatacttt atctgctgat 30000

gtaaccgcca taatatcctt aaatccacca tcggtcaaaa cttgggtatg acggtgaagg 30060

caagccttac ctatctggcg taagtcttta cgttctttct taatatcgtc ggggtccatg 30120

ccaaacatct tacccgccgt tacgcagtaa atatccagcc cggcgcggaa cgtatcaagc 30180

gcggtttctt cgcccgccag ccacgctaaa ccacggcctt cgacgttaga gtaatcggca 30240

acgacaaact tatgccctgc ttccggaatg atgcagctac ggacggtaga cgctgttagt 30300

tttgccacgt caaaccggcg atgcgcccgg cctttaagta acgcggcaat acccttatca 30360

agttcatcgt cgtgataata cccgcgcgcc aggttttgcg gctgaaaacc tttccccgcc 30420

caccgcaatg ttcgcttcgc tcctccgtat tgcaggcaac cacggcggcg gtcatctgaa 30480

gaacggccta acagcaacgg cgcgtatttc gtcgatgcgg tagaagcagc cccgagacgc 30540

atctcgataa tagtacgcgc atcatccggt aaatcctcat ctgccagcaa gtcgttaagc 30600

gtcgacttct gtgcgttgtg gatgcggtgt gccggtgcta gttcctgtaa aataggcaag 30660

aagtctttgc cggttagtga accgccatat ttacgttggg cttcttcctg taactcttct 30720

ttatggcgcg tgactgcttc aatcgctgct tccgccaatg ccacatcaac cttaaatcct 30780

cggtcattaa tcacctggtc taagtgcagc acgcggtctt cgaactcaga attcccccac 30840

ttaggcattt tcttatacac ttcacgcatg gacgtaatgt cgctttttgc gtatgcaata 30900

aattctgccc actcttttgg gtgggtatcg gcagtataac gtcgaatctt atagttcttg 30960

ggcgtaggct tactgaatcg ctgtatcagc gccttaccgc gtttatcctt agccatgctg 31020

gcgtcaatgt ttaaaacctc gcacaacgca gccagtgaac cagggagagc gtgtcggaac 31080

gcgactatca tagtgtctat aatgttttct accaggagtt cgaatcccca gcaatgctta 31140

ataactggtc ggtcgaacat aaggaagttt tggcctacca gtttgaggtt gctgtctggt 31200

ttttgcagct ttagcatcgc ccggcgcaaa tcacgcggca tatcactccc gtcggtggcg 31260

tcccatactt gcactgggcc ttcatcgaaa gcgtaggtgc aaataacaat ttcggtagtt 31320

ggatgctcag cgtaggcata ggaaccgact tttttcaaat cggcttctga gaatgtttca 31380

gtatcgagat aaagcagatt catttatttg acccttatag aaaaggcgac cgaagccgcc 31440

ttaattgaaa agaaaatata ttaacggtta cgacggcgtt cgcgacgcgg tgtttcgtct 31500

tcatcatctt ccaggtcgtc aacgctggca gaaaccgcag aaccaccgaa tgcctttcct 31560

tcacctgcaa atttgatgcc gtttagtttt gcgcctaaaa ctttatattg ctcggaaaac 31620

cagatttcga ttgagatatt agccacacaa ccgctatata cctgcttgcc ttcaatctgt 31680

tcaccgtcga tgttgaagtc tggttctacc tgcttttcac ccttaaccga agtcaggatt 31740

aatggctgtt gtttgttctt ggcctggaag taaaaacctt ccgggaagtc ttcaaacggg 31800

ttatcacgtt cggcgatgtc gcgcacggcg catttatcca tatgcttacc ttcgccgtag 31860

ttctgcttca tccatttctc agcggcggcc tctcccaatg cttccgacac tacggcaaat 31920

actgtatcgc ggagttcttc aatttgcgga tgttccggcg taagaataaa agtaccgtta 31980

tacgtacctt tagtaatgga gccatcgcta ttttcacggt ctttagcgcg ttcgaatacg 32040

ttcagccatg cggtttgtac tttacgaaga ttaagtttga ttcccatttt agttttctcg 32100

cgttttagag tttatccggg aagctgcccg gccagtgatt aagaatataa tagagtacga 32160

ttaagatgtc aagcctctaa atcgtcttca gtaacatttt tccattctgg ccgtttgtct 32220

tcgaccgttg tcacacatgg cgctcctggc ttacgggtta cgtgtttctc cagcgccgcc 32280

catagctcag aacctttgtg ctgcttctcg gcctcggtcg gggtcattag tacttcttta 32340

tgaagcattt cagcgcccaa ctcaaaatgt gatagcgttt ccagcagcgc atcggtatct 32400

ttccacgcac gattacccgg gcggccttca accagcttgt accccggcac tttcttaccg 32460

gaatgcaacg cggcagccat cgctttctcg accttgtcga tgtgctggcg cagcagtggc 32520

aacttctcat actcagctac gagttgctcc ggtgtaagtt ccagcgcaaa gtcgtcctcc 32580

agttcttccg ccagtacaga gttaacggtt tttgtacgcg cggagcactg ttccgagaac 32640

cgacaccact gacaaccatc gacagacggc ttgaagtccg acgctttcag attcttcttg 32700

ccacggaaat atgcatcaag agccaacagt gcgcgtttct gcgcgaactt agcgaacagt 32760

tccagacctt caaccgagat gtcccactcc gacgcaccgc cagcgtacgg ctggaagatg 32820

accagacgaa caacggttat gttatagcgt ctcttgagtc tgcgataaac accgagagcg 32880

taaagcataa gctgtttgtt ttctttcgct tcaacacgat gccgtcctgt tttcaggtcg 32940

ccgataatga gcatgtgctc gtctgtgttc gccatctccc gaacggcaac aaggtcggcg 33000

gtcccgaacg tctcaacacc ttcgtatcct ggatgtaata cctcagtaag attgacacgc 33060

atttccagct tggcgtaagt tgctacgtcg attatagctt tgcagtagtc ggtgtacttg 33120

cgcacctgct caatcatgtc tgccgtaatc agtaccgcac ctttcatcgg cttaattaat 33180

gctttaacag cgcctttacc ttcctccagc acatacgcac ctacatcgcg ttctaacggc 33240

aacgcagtgc cgcgtatata ggcgttaagg tgcatctccg ctatggtgtg catagctgtg 33300

cctaataccg cagctttacc tgacgtgtta ggaatatctt tttcacaggc cagtgatgca 33360

gcgcatgaca gccatttttt agcacctgac ggcgacagta aggcgtgaac atcgttattg 33420

ccgccgcgtt cttttagaat catcgtaaac tctccaaata taagtttaag gcctgtaact 33480

gttcttctgt gggaatgaaa gaatcatcgc gatgtaagtc gcttaactgt tgcagcattc 33540

ccgcgaactg acgtcttgcg tcaacagcga ccatgtgatg cttttcactt ttgtatatta 33600

atgttttcgg gtaatcctca agcctaccag acgatttatt cacgcggagc gcccgagctt 33660

catgcgcaat gtgtatgtac tttttgccgt tttgtagcac agtagctaca ccgctgttct 33720

ttctgcggtc gccgacggtg taaactttgt cgccgctgcg caaattaagc caccatttat 33780

cagtcatgct ctgttctccc actggtcaat taaatgtctt gtcttatgct cgcaatgcat 33840

agcccaccca tacatcgact cgaagacata aaagtcaggc tttgcgaatg tcgtgcgctt 33900

aatctgtgac acgtgacggc caatatcttt aggtcgcggt aacttgccta agtacgccat 33960

ctcttccatc tggtgcgcac cggacggcgc acgcaacagc cacaacgcct ctgtgttatc 34020

ccgcctgtcg acggcgcggt atagttggta aatcataacc ccgaccccca gttaaagcgg 34080

cccgaaggcc gcaggatagt tattcttctt cgaaatactt gttcttgatt gctgtcaggc 34140

gctccagata ctcagccagg tcttcgtctt taatcgctgc aatcttcatc ttcttaccgg 34200

taaactcttc cagcagttcg tcagaatcgt cgcacgcggc atcgcttggg ccttcgttaa 34260

tcgcatcgtc gatagcctga atctggtcgc gaagagattg gtaatcaatt tcttccttct 34320

cttcttgtac tggttcctct acttttgcct tacgcggctt acgttttggt ttctcctctt 34380

ctgccggttt agtgtcgacg atgtcctcac cttcgaccgg aatttctttt gctttctcga 34440

cttcttccag tgctttagcc agttcttttt taactgtctc gacaccggca acggagacgt 34500

caacagtcat ttcgcttacg gatttgttag acgtctgttt cacactattc gcagcaatca 34560

gttcatgggc gactacgaaa cgttcaagta attttaagaa ttggtctaac attatttctt 34620

ctcctgttta gttggtgata aagactataa tcgtactcta ttatttatgc aagcactttt 34680

tcgtaaattg cgtcgtgtat tttattactg tactattact gcatatctaa ctaaggagta 34740

aacatatgca gcaatctgaa ttaggcgctc gcgtagaacg ccgccgtaaa gaaatcggca 34800

tgggtcaaac tgagcttgct ttcaaagctg gcgtatccca aagccttatt acccatctgg 34860

caaccggtcg ggtgtgtaag gtagactgtt ttaaaatctt ccacattgcc gatgctctcg 34920

gtgttgaccc tcgatggtta agctttggtg atacaggggc ctgatggccc ctttcttttt 34980

actctaaatc cccttctgtt gtctttatgt tgtcgttagg ttcatacctg ttctttggcc 35040

gcttacggtc atctataccc gtcggcaacc tgtaagtatt cgttacaact tcaccattct 35100

catcgcaacc aagcatcaat tcaccctctc gtagcatctt ctcgagcacc aggtttgtta 35160

tgctgtggtc cccggccctg gcgactattt gccgttgtgt gaaacctcgt ccggtatcat 35220

cggcttgctg taaatcctcg agagcagaca taaccgattc tcgtgcggaa gcatctttag 35280

accgtttaac tgtatccttt acgtaatctt tacctttacc gtccagtcct tcgttgcgct 35340

ctttttcctc gtccgtttcg aacggttgga agccccacgg catgagtaca agagctttat 35400

gcggttccgg caggtctagg ttaacgattt ccccgtatcc ctcgccccca acgaattcta 35460

cagcctggaa ctctttcggt ggtggagctt cgcgaaactg taccggttcc agcaccatac 35520

ctattgtttt ctgctgcata ccgtttttgt tcttggtatg tgcaacgttt atttgtttct 35580

cggtagcgcg gacaagcgtt agttccacat cgacaccggc gtacagcgcc ccacttccgc 35640

gagccttact cccgccttta ggagtatggt gaacgacacc cacagcgcct ttagtaccgt 35700

cgcgtacttc tttgagcatc gccactaccc gccccatccc gtcttgtccg gctgagttct 35760

cgttaaattt gtctatccag ttcccgaatg tctggttaag agtgtcgaaa gcgaccatcc 35820

cgacaggttc actgcctgct agctggcgca ttttacgcac cagtttcttc gtatccgcga 35880

actcaccggc atccagaaca tgcacatacc gcataccttc atcgccgtat ttagcggcca 35940

acgcagcgat acgggtgtgc gtgaactccc caccctcacc gtcgatgtag aagtggtgcg 36000

ctttacgggt atccgccccc gcgaaccgat acccggcagc actgatatac atcatgccga 36060

gtgtgtagaa cgatttatac gtcccggatt caccgacgat atcccatatg cagttagacg 36120

gcatatatcc ttcgacaatg aagtcagcct gtggtgctgg cggttcttcg tcggccaggt 36180

cttcttcgtc gcaagtgacc ccctcggccc acccaagcgc cacctccaca cggtcaaaag 36240

gtaagccggt ggccgcacaa gcgtaagccc acatatcccg tccgccaggc ttcatcccgc 36300

ccgtagctac aaggtcggta tcgtggtaca gggtaacgtt gggtcgctcg aagccgtcgc 36360

gcggccaaca gaacaggaag tcgtcctgtt tcggttctcc cgtcgagtat tgggcggcgt 36420

gctccggcgt ggcgggcatt tgcaggccgc ggtcggtcat acggccgccg aactcgaagg 36480

cgaactcttc aaacaggtca gttaattcgg attgctcacc ctcgggcact ttgtaatcgg 36540

aagcgcctgt aacgttaatt tcaggaactc cctccataag cttactggcc gtaatcatgc 36600

ggctactttc ggacacgatg acctgacttc ccactggcgg gcggtacatc ggctgagaca 36660

gggtgaagcc cgcgctatct acgtcacggc ctttaaggaa atgcgtcagc agaccgtacc 36720

ggatgcggat gatgtctcca cctgttacag gagtgcgtac cggcataacg acgcgatagc 36780

gcggcgcttc ttcggtgtgg gacgccgtgg tgtagagcat cattgcgtaa cgcgactccc 36840

gcaccatctc gcaatcagtg gcgaattctt ccggcgtagc actatccacg tcggcgtaag 36900

ccagggacga agacgttacg gacgcattgc agcgataaaa cataccttcg gcggcttctt 36960

tacctgtaga gcttaccgtg gcagtacacg cggcggtgat atatccgggg tctgtcttgg 37020

ggttccggcg cgaacgttta agcggctgca taagttcaac gaactcatcc caggtgccgg 37080

aagtcgtggt gtaaacgtta atgtcagcgc gttcctcacg gcgattgcta cgcgaccatg 37140

agtatgctaa attacctgtt gacatgtgct tttcctttgt agtgtttaag gccctgacgt 37200

tcgcgcgttg gggcttttct tttattcaag gtcttctggc gtcgcctgat agcctttaac 37260

ttcagctact gccgaatatt taccggcctt acttttaact gctccgtctt taaccagaac 37320

ctttaacgtg ttctcaatga gagaggaact atagtactga aaataagtcc ggcgcaagtc 37380

aaggacacta caagacccct ttttaaatgt aagagcagct acagccctga atactctttt 37440

ctggaaatca gtcacttcac attctcccta atccacgctt ccactttctc cctgtcgaat 37500

gttcccggca tccggcgacc cataatccgg acacaacaat ccgggaactt gccgtctctt 37560

aaccagttat tcagcgtgcg gcgggtaacc ccaataagct cagctacttc attctgtgtc 37620

attatcaaac cctccatctc agttagtaaa gcaagtatac ccacggcata atgggaaatc 37680

aactataaac acacctattg acattttcat ttttttcgtg taagttgtct cttgtaactt 37740

caagttactt aagtttctgt ttcactttgt gagagcttag cgggtccgcc ctaagcggac 37800

acgcgttaag gcttactgaa acccgcaaac ttaaaactca gtaacttcct tccttagtat 37860

ctgaactgcc ttgggcgagc tatattctca tcggcagcat atctcttaag tatctgaatt 37920

ccttgatgtg ccgtagattc gcaaccgtaa aatgtaacga ttagtgggca ggttttagac 37980

ctgcccttta gttaccaggt aataagaata aaggcacaac cctggttgaa gaaccacaat 38040

ggcaacgtaa ctgaaatgcc gaaagattct tagtctacta ctcagggatt aacgcacttg 38100

cctaacggca agcgcttacc tgacgaggga cgggagacaa gaatagaaag gcacttgcag 38160

aaccaaatat aatagagtac tattatcgca ggttaacgag aaaagaggat gagtcatgtt 38220

taagaagggc cagttagtgc ggtgtactat ctctcacgaa tattacatag ttcttgacca 38280

gataagaaag atgttagctg tcaggagtgt aagttggggg cgtgccgggt ttatggacgc 38340

gcggtatgta acgttaatcg gaaataacta cacggcaaaa gattctaaag agtctgcgtc 38400

ggtgaatctc gggtcttaaa ggaggttgcc gtttatgaag aacttgcagt tggccgtgaa 38460

aagtgaatat ttcgatgcca tgattcgcgg ggagaaaacg gaagagtatc gcctggtaaa 38520

tgattattgg tggacgcgcc ttgttggtcg tgaattcgac cgtttgatta taacgaaggg 38580

ttaccctaaa cgggacgata agagcaggag aatagagtgt aagtaccgtg ggtatgagat 38640

taaagaaata acacacccgc atttcggcag tgaaccagtg gaagtttttg cgataaaggt 38700

ggagttatga gcaagtggat taaatgtagt gagcggatgc ctcctaaaat gacaggggtt 38760

ttagtggcta cggaattcgg gcataaaggc gactggagaa tgaagtaggc cacttatgta 38820

ccggagcatc ctggcgctga cgatgggtgg ctgattccgg gggcataggg gaaaccgtcg 38880

cactggatgc cgttaccgga accgccagaa gtgcgatgtt atattgtaac aactatttag 38940

gattttactg agtgacttac gctgctgagt agcagcgtac catttttgtt aatatttttc 39000

tatttcacca aacgcaacaa tcgaagcgac tgttgcagga gacgcaacaa tgaaaccgaa 39060

cgacctcgta acctggaccg gacgtaacgg agagaccaga cacggcaaaa taacgtcgct 39120

tcacggcatc tatgttcgcg tcgagtggtg gcgggcccat gctaagaagc ctcgtcgcat 39180

tctgacgcgt caggatagat taatcgcgaa atagtattga cgtaaccaaa cataatagtg 39240

tactattact ttattgaaac gaaaggataa tcaaatgacg agcatacttt tcatatgggt 39300

cgtgtccgct ggtcagctac aactggccgg gacagaaacg ttttacacgt tagaggcgtg 39360

ccaggtagcc gcacgcgctg cggagaacgc gccgttaact ttcgtagagc gtcctgttga 39420

cgctcaggta cgcggtattt gcacactgaa acggttatct aagcaggggg agagatgagt 39480

ctcgcaaccg atatactgaa acacgccggc atcaacctgg cacctccttc atcggcggta 39540

acaaagaagg ttactcgcgt taaagaaaag gttaaacgca aacctaagcc gcgtgtaaag 39600

ccggttaacg agatgcctga cgtctatccg cgcatccccg gagtacacca gcctaaatat 39660

tgtgtaggta aaggtctgtg gcgcgcgcac tcttacgatg gcaagaaagt ggtaaacctc 39720

ggtgagttca gtagccaggc aagggcgcat atggcggtta aactgtacaa actgtggcgt 39780

aaacgtgggt attccgaaat cccgcacaag ccatctattc gactttatac gttcaggtaa 39840

tttatatgac tacgatagct ttcgacggca aaaccatggc ctgcgataca tgtgtcacgg 39900

ggaattttaa atactatacg gataccaaga tttacgagaa cgaccacttt gttatagggg 39960

tatccgggga cgccggggtg ggtatgttgt tggttgccga tgatgagata ctgacgccta 40020

agcactacga cttcgacttt tcggcgctgg tgtttgttaa agaggataac cgtattttca 40080

gggtagaatt cttcaagtca tgggattcgc cgttaagctc agttatacca attgcgggta 40140

acgcagcggc cgttggctcc ggtgctccgt atgcacttac ggcgatgttt atgggtgcca 40200

ccgctactgc tgctgtcgag atagcgaagc gcttcgaccc taatactgat gggggagtga 40260

taacacatcg actaggataa atccgaatta tctgttacta aaattgtcgt ataaatgtta 40320

atctccgaag gtaaactaac tttcggagat tttttattgt ggacgataaa tacctttggc 40380

ttagtgtggc gggcctcgcc gggggtgctg tatctcagat taagaagcgc gaggctattt 40440

caccgtggtt gagattgtgt cacctggttg cctcggcatg ttgtgcggtg tacgcatccc 40500

ccatcattat aagttactat gaattatcac agtctgaggg tcagtacctg gttcctttcg 40560

gcgtaggtat gttctggctt aaattatttg aggccgccga ctcgtccctc agcaacttta 40620

agttaccgtg ggggaaataa catgctcaac tcacaaatcg gcgccatttg cctggtcgca 40680

atcatcatca cctcgttaat taatatctac gcgcactgga ttgaagacgg tcttttcggg 40740

cgtctgttgt atatggcgtc agtagtgacc gccgccggag gtctcgccca tctctttacc 40800

ggtgccattc caccgttcat aattacgact ctcgtcgcta tattcgcgct taaatcggtg 40860

cgtcatattt gcgtgaaagg cgcccgctac tacaaatacc ggaggatgta tgtcaaaccg 40920

aaacatcagt gacaacgggt tacacttcac cgccgcgttc gaggggttcc gaggaaccgc 40980

gtacaaggca acgaagaacg agaagtacct tactattggt tatggtcact atggtgccga 41040

tgtgaaagaa ggtcagaaga ttaccgaagg ccagggcctc ctgcttctgc ataaggatat 41100

ggctaaggcc gtagctgcgg tagacaccgt tgcgcatccg tctctaaatc agtcacagtt 41160

cgacgccgtg tgtgaccttg tgtataacgc tggtgcaggt gtgattgccg cttctaccgg 41220

tacaggtcag gcgctgcgaa aaggcgatgc atctacactg agaaacaaac tcagccagtt 41280

ccattaccag aacggcaaat cactcctcgg tttgcgacgc cgcgctgctg gtcgtgttgc 41340

actgttcgac ggtatgttgt ggcaacaggc cgaagctgta gggcgcgctg cgaagtaggt 41400

tgacacatag gagtattcct aagatactaa acctgctcct gcttattcat ccctctagct 41460

cctttatccc ggtaactgac cctaccggga ttttttttta tctgtatcct gaaataatag 41520

ttgactagta acattaaccc tattatattt agttcatcga caacgagaac ggagtagaga 41580

agatgaactt acaaagtgat aaagtttttt accattgcaa accactactg gacaccgaag 41640

catttaaaga cgcgcaactg atggcacgca ttgccgttaa taacctaagt acacggattc 41700

cagcggatgc gttctggttc gccgcgatgc agacactgaa agcagcttat gcaggagaac 41760

aaaaatgaaa gtatatatcg tatccggatg gcattactac gaggacactt acgttcttgg 41820

tgtttatctc agcgaagaaa aggccgaagc cgccgccgcg gtggaacgaa cctcctatga 41880

ttacgacggc gtagacgtat gcgaatggga ggtgcaggaa tgagcgacaa cggacaagta 41940

gcagtaacgt ttaaggtggg cggtaaggta agtaacacgc ccttcccgac acgcgaggaa 42000

ctgattaaac gtaacagttt cccggggccg gacaagaaca agtatctcaa tcgaatgtgg 42060

ggtgagaaga aatgagacgt aaaccgttgc caaaagttgg cgacacccgg ctaacccccg 42120

agttgagaag gctaaaaata acagcagttg tccatgaaag acactcaatg ccgcagttag 42180

atgttaaggc tgtttggtgt ctgcatttta acgatggtac ttacgagtac gttgatgagg 42240

atgagtttta tgactaacaa cgaatacgaa aagatgatgg tagaagccgc agacggcaaa 42300

ttcaagattg aagccgtaga cgcgcaaatt aaggcacacc aggctgcgct tgactacctt 42360

aacgaacatc gccgggagct tatcaatcgt tatgacctga ataagtgtac gcacgatttt 42420

gattacaatc ctaaagatat gtgcggacag tgtgttaagt gcggggcgat taaacgatga 42480

aacgagtatg taaagagacg ttgcgcgagc gaattatgta tctggagtcg gtaagtaagt 42540

taggtctgtc aatgaacgaa gaattccagt tagaagcgta taagatgcta ctgacccggc 42600

ttgagaagga aagcgagcaa aaatgattac cagtatccca aaactaatag agacacacgg 42660

cacattagcg gatacgtgcc gggagacggg acttaatgag atgacattat ccaagtaccg 42720

ccatgacaca aaatgcgagc aacatgtcat ctataacaac cgactgatga cccatacgaa 42780

aacgtcaccg gtcatctaca cgaaacgtgg attatcgcgc aacgaccgta tgaagctgga 42840

ggggtgatgg atagttttct catcctcatg ggtataggat tcacaacatt actgcttgcc 42900

gttgttacat acctgatgtg gattttgttt ctttggccat ttgttgaggc ggtaagtctt 42960

acaaggatga cgagagagta ttggcgtatt aatgggtgca agcgaggtgc ctggtgtacg 43020

atacgtttgt tctttacgtg gtatgctgaa acagtatttg gtcgtaggtt cgaagccata 43080

catagtcgcg ggtggcgatg ggaagccgta ggtaaatggt gggtttataa agaggagagt 43140

ggagaatgat tgagattttg aaggctaccg attccactgg cgacctgatg ttttggtttg 43200

cctttctcag tgcgctaata aaacaaccga ctgtgtttaa cagtcttccc cgatatctgt 43260

ggtggcctat gtttttaggc ttgggtgtat caggagcggc cggcatcctg ctgtggtttt 43320

tcaaatgaga ttactaatca tcccgaacgc ctgggttatc gccgcagcta acgaccatta 43380

cggcggcgat ggtagaagag cactacgaca cggcctatat aactgacaca agcccgcata 43440

tgcgggcttt tctgtacaca ggcctatctt tccctataca atc 43483