阅读说明：本技术 一种犬猫用可减重降脂和调节肠道菌群的组合物及其制备方法 (Composition capable of reducing weight and fat and regulating intestinal flora for dogs and cats and preparation method thereof ) 是由 陆江 朱道仙 刘静 卢劲晔 卢炜 刘莉 郝福星 吴植 周红蕾 于 2021-08-25 设计创作，主要内容包括：本发明公开了一种犬猫用可减重降脂和调节肠道菌群的组合物及其制备方法,本发明涉及畜牧兽医养殖业技术领域,包括菊粉、银杏叶发酵物、荷叶发酵物、鸡肉粉和麦芽糊精,具备了利用益生菌发酵银杏叶和荷叶,制备了银杏叶发酵物和荷叶发酵物,按药食同源原理,配合菊粉极大提高了组合物的减重降脂和调节肠道菌群的作用,经济又高效,并配合鸡肉粉既提高了组合物的适口性又补充了蛋白营养,稳定性好,操作简便,无副作用的效果。(The invention discloses a composition capable of reducing weight and fat and regulating intestinal flora for dogs and cats and a preparation method thereof, and relates to the technical field of livestock and veterinary breeding industry.)

1. The composition capable of reducing weight and fat and regulating intestinal flora for dogs and cats is characterized by comprising, by weight, 10-20 parts of inulin, 5-10 parts of ginkgo leaf leavening, 5-10 parts of lotus leaf leavening, 30-40 parts of chicken powder and 5-10 parts of maltodextrin.

2. The composition for reducing weight and fat and regulating intestinal flora according to claim 1, which is characterized in that: the composition comprises, by weight, 20 parts of inulin, 8 parts of ginkgo leaf leavening, 8 parts of lotus leaf leavening, 35 parts of chicken powder and 10 parts of maltodextrin.

3. A preparation method of the composition for reducing weight and fat and regulating intestinal flora according to claim 1, which comprises the following steps:

s1: weighing 50g of dried ginkgo leaves, crushing to 40-60 meshes of fineness, dissolving in 150ml of deionized water, and fully dissolving to prepare a dissolving solution A;

s2: weighing 12g of bacillus subtilis dry powder, adding the bacillus subtilis dry powder into the solution A, fully and uniformly mixing, and fermenting to obtain fermentation liquor;

s3: centrifuging the fermentation liquor to remove the precipitate, reserving the supernatant, and carrying out cold drying on the supernatant to obtain a ginkgo leaf fermentation product;

s4: preparing a lotus leaf ferment according to the method of S1-S3;

s5: weighing maltodextrin, dissolving the maltodextrin in 8 times of water by weight, and fully stirring to prepare a solution B;

s6: weighing inulin, folium Ginkgo fermented product, folium Nelumbinis fermented product and chicken powder respectively, adding into solution B, stirring to obtain paste, cooling, and sterilizing to obtain the composition.

4. The preparation method of the composition for reducing weight and fat and regulating intestinal flora according to claim 3, wherein the composition comprises the following components in percentage by weight: the fermentation temperature in the step S2 is 28 ℃, and the fermentation time is 72 hours.

5. The preparation method of the composition for reducing weight and fat and regulating intestinal flora according to claim 3, wherein the composition comprises the following components in percentage by weight: and in the step S3, centrifuging at 10000r/min for 20 min.

6. The preparation method of the composition for reducing weight and fat and regulating intestinal flora according to claim 3, wherein the composition comprises the following components in percentage by weight: in the step S6, the mixture is stirred for 30min at the temperature of 75 ℃, and then the temperature is reduced to 45 ℃ while stirring.

7. The preparation method of the composition for reducing weight and fat and regulating intestinal flora according to claim 3, wherein the composition comprises the following components in percentage by weight: the sterilization mode in step S6 is ethylene oxide sterilization.

Technical Field

The invention relates to the technical field of livestock and veterinary breeding industry, in particular to a composition capable of reducing weight and fat and regulating intestinal flora for dogs and cats and a preparation method thereof.

Background

In china, dogs and cats have become a member of many families as partners as people's standard of living has improved. However, because of bad behaviors or bad dietary habits, the incidence of obesity and related metabolic syndrome of dogs and cats increases year by year, the incidence of obesity of dogs and cats is also at a high level in the global scope, pet breeders have recognized health problems brought by obesity to dogs, the obesity can cause imbalance of intestinal flora, the imbalance of the intestinal flora induces excessive energy intake of organisms, and further the organisms accumulate fat in the bodies to aggravate the obesity.

Disclosure of Invention

The composition has the advantages that ginkgo leaves and lotus leaves are fermented by probiotics, ginkgo leaf leavening and lotus leaf leavening are prepared, the effects of losing weight, reducing fat and adjusting intestinal flora of the composition are greatly improved by matching inulin according to the principle of homology of medicine and food, the composition is economical and efficient, the palatability of the composition is improved and protein nutrition is supplemented by matching chicken powder, the stability is good, the operation is simple and convenient, the effect of no side effect is realized, the weight-losing products for pets in the current market are solved, chemical medicines are mainly used, the rebound is easy, and certain safety troubles are caused; and some products containing Chinese herbal medicine raw materials have poor taste, low bioavailability of the Chinese herbal medicine and unsatisfactory effect.

In order to achieve the purpose, the invention provides the following technical scheme: a composition capable of reducing weight and fat and adjusting intestinal flora for dogs and cats comprises, by weight, 10-20 parts of inulin, 5-10 parts of ginkgo leaf leavening, 5-10 parts of lotus leaf leavening, 30-40 parts of chicken powder and 5-10 parts of maltodextrin.

Optionally, the optimal effect formula of the composition comprises 20 parts of inulin, 8 parts of ginkgo leaf leavening, 8 parts of lotus leaf leavening, 35 parts of chicken powder and 10 parts of maltodextrin.

The invention provides a preparation method which comprises the following steps: a preparation method of a composition for reducing weight and fat and regulating intestinal flora for dogs and cats comprises the following steps:

s1: weighing 50g of dried ginkgo leaves, crushing to 40-60 meshes of fineness, dissolving in 150ml of deionized water, and fully dissolving to obtain a solution A.

S2: and weighing 12g of bacillus subtilis dry powder, adding the bacillus subtilis dry powder into the solution A, fully and uniformly mixing, and fermenting to obtain fermentation liquor.

S3: centrifuging the fermentation liquor to remove the precipitate, reserving the supernatant, and carrying out cold drying on the supernatant to obtain the ginkgo leaf fermentation product.

S4: preparing the lotus leaf leavening according to the methods of S1-S3.

S5: weighing maltodextrin, dissolving the maltodextrin in 8 times of water by weight, and fully stirring to prepare a solution B.

S6: weighing inulin, folium Ginkgo fermented product, folium Nelumbinis fermented product and chicken powder respectively, adding into solution B, stirring to obtain paste, cooling, and sterilizing to obtain the composition.

Optionally, the fermentation temperature in the step S2 is 28 ℃, and the fermentation time is 72 hours.

Optionally, the centrifugation is performed at 10000r/min for 20min in the step S3.

Optionally, in the step S6, the mixture is stirred for 30min at a temperature of 75 ℃, and then cooled to 45 ℃ while stirring.

Optionally, the sterilization mode in step S6 is ethylene oxide sterilization.

Compared with the prior art, the invention has the following beneficial effects:

firstly, the ginkgo leaf leavening and the lotus leaf leavening are prepared by fermenting the ginkgo leaf and the lotus leaf by utilizing probiotics, so that the defects that the traditional extraction process is complex and a large amount of active ingredients are lost are overcome, and active substances in the ginkgo leaf and the lotus leaf are better and directly released.

The chicken powder is added, so that the palatability of the composition is improved, the protein nutrition is supplemented, the stability is good, no side effect is caused, and the pet is willing to accept the chicken powder.

The inulin is added, so that the weight loss and lipid reduction effects of the composition and the effect of regulating intestinal flora are improved, the composition is economical and efficient, the growth and the propagation of beneficial bacteria in intestinal tracts can be promoted, and the growth and the propagation of harmful bacteria can be inhibited.

Drawings

FIG. 1 is a process flow diagram of the present invention;

FIG. 2 is a graph of the change in body weight and body fat rate of the present invention;

FIG. 3 is a graph showing the change in the number of intestinal flora according to the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In a first embodiment, please refer to fig. 1 to 3, the present invention provides a technical solution: a composition for reducing weight and fat and regulating intestinal flora for dogs and cats comprises, by weight, 20 parts of inulin, 8 parts of ginkgo leaf ferment, 8 parts of lotus leaf ferment, 35 parts of chicken powder and 10 parts of maltodextrin.

Referring to fig. 1 to 3, the present invention provides a method for preparing a composition for reducing weight and fat and regulating intestinal flora for dogs and cats, comprising the following steps:

s1: weighing 50g of dried ginkgo leaves, crushing to 40-60 meshes of fineness, dissolving in 150ml of deionized water, and fully dissolving to obtain a solution A.

S2: and weighing 12g of bacillus subtilis dry powder, adding the bacillus subtilis dry powder into the solution A, fully and uniformly mixing, and fermenting to obtain fermentation liquor.

S3: centrifuging the fermentation liquor to remove the precipitate, reserving the supernatant, and carrying out cold drying on the supernatant to obtain the ginkgo leaf fermentation product.

S4: preparing the lotus leaf leavening according to the methods of S1-S3.

S5: weighing maltodextrin, dissolving the maltodextrin in 8 times of water by weight, and fully stirring to prepare a solution B.

S6: weighing inulin, folium Ginkgo fermented product, folium Nelumbinis fermented product and chicken powder respectively, adding into solution B, stirring to obtain paste, cooling, and sterilizing to obtain the composition.

In a second embodiment, referring to fig. 1 to 3, the present invention provides a technical solution: a composition for reducing weight and fat and regulating intestinal flora for dogs and cats comprises, by weight, 10 parts of inulin, 5 parts of ginkgo leaf ferment, 5 parts of lotus leaf ferment, 30 parts of chicken powder and 5 parts of maltodextrin.

Referring to fig. 1 to 3, the present invention provides a preparation method of a composition for reducing weight and lipid and regulating intestinal flora for dogs and cats, comprising the following steps: the specific preparation procedure was the same as in example 1.

In a third embodiment, referring to fig. 1 to 3, the present invention provides a technical solution: a composition for reducing weight and fat and regulating intestinal flora for dogs and cats comprises, by weight, 20 parts of inulin, 10 parts of ginkgo leaf ferment, 10 parts of lotus leaf ferment, 40 parts of chicken powder and 10 parts of maltodextrin.

Referring to fig. 1 to 3, the present invention provides a preparation method of a composition for reducing weight and lipid and regulating intestinal flora for dogs and cats, comprising the following steps: the specific preparation procedure was the same as in example 1.

In a fourth embodiment, referring to fig. 1 to 3, the present invention provides a technical solution: a composition for reducing weight and fat and regulating intestinal flora for dogs and cats comprises, by weight, 15 parts of inulin, 7 parts of ginkgo leaf ferment, 5 parts of lotus leaf ferment, 35 parts of chicken powder and 8 parts of maltodextrin.

Referring to fig. 1 to 3, the present invention provides a preparation method of a composition for reducing weight and lipid and regulating intestinal flora for dogs and cats, comprising the following steps: the specific preparation procedure was the same as in example 1.

In a fifth embodiment, referring to fig. 1 to 3, the present invention provides a technical solution: a composition for reducing weight and fat and regulating intestinal flora for dogs and cats comprises, by weight, 15 parts of inulin, 7 parts of ginkgo leaf ferment, 5 parts of lotus leaf ferment, 35 parts of chicken powder and 8 parts of maltodextrin.

Referring to fig. 1 to 3, the present invention provides a preparation method of a composition for reducing weight and lipid and regulating intestinal flora for dogs and cats, comprising the following steps: the specific preparation procedure was the same as in example 1.

In a sixth embodiment, referring to fig. 1 to 3, the present invention provides a technical solution: a composition for reducing weight and fat and regulating intestinal flora for dogs and cats comprises, by weight, 20 parts of inulin, 5 parts of ginkgo leaf ferment, 8 parts of lotus leaf ferment, 40 parts of chicken powder and 8 parts of maltodextrin.

Referring to fig. 1 to 3, the present invention provides a preparation method of a composition for reducing weight and lipid and regulating intestinal flora for dogs and cats, comprising the following steps: the specific preparation procedure was the same as in example 1.

Further, the optimal effect formula of the composition in the embodiment 1 comprises 20 parts of inulin, 8 parts of ginkgo leaf leavening, 8 parts of lotus leaf leavening, 35 parts of chicken powder and 10 parts of maltodextrin, the palatability of the composition is improved and protein nutrition is supplemented by adding the chicken powder, the stability is good, no side effect is caused, pets are willing to accept, the effects of reducing weight and fat and adjusting intestinal flora of the composition are improved by adding the inulin, the composition is economical and efficient, and the growth and the reproduction of beneficial bacteria in intestinal tracts can be promoted and the growth and the reproduction of harmful bacteria can be inhibited.

Further, the composition of example 1 can be used for prevention and adjuvant treatment of obesity in dogs and cats.

Further, in the step S2 of example 1, the fermentation temperature is 28 ℃, the fermentation time is 72 hours, and the fermentation temperature and time are controlled to avoid the fermentation failure and reduce the working efficiency.

Further, in the step S3 in the embodiment 1, after centrifugation is performed at 10000r/min for 20min, macromolecular substances such as bacteria and proteins in the fermentation liquid can be well removed, which is convenient for subsequent use.

Further, in step S6 of example 1, the temperature was kept at 75 ℃ for 30min under stirring, and then the temperature was decreased to 45 ℃ with stirring, so that the drugs were mixed uniformly with stirring, and the temperature was controlled to prevent deterioration.

Further, the sterilization mode in step S6 in example 1 is ethylene oxide sterilization, which can kill all microorganisms including bacterial spores, and the sterilized article can be wrapped and integrally packaged, and can be maintained in an aseptic state before use, and can penetrate the article with irregular shape and sterilize, thereby ensuring the quality of the ointment.

The composition which is designed and prepared in the embodiment and has the effects of losing weight, reducing fat and regulating intestinal flora for obese dogs and cats is verified by clinical animal tests, compared with the embodiment, the composition has better effect, and the clinical test effect is as follows:

1 materials and methods

1.1 design of the experiment

60 male Beagle dogs, about 2 years old, were selected and divided into 2 groups: the normal diet control group comprises 5 normal diet control groups and 55 high fat diet groups, the normal diet control group is fed with normal diet, the high fat diet group is fed with high fat diet containing 30% lard, after 8 weeks of feeding, 40 test dogs with the weight increased by 20% compared with the test dogs are selected from the high fat diet group as obese dogs, the test dogs are further divided into an obesity model control group, an inulin control group, an example 1 group, an example 2 group, an example 3 group, an example 4 group, an example 5 group and an example 6 group, all the obese dogs are further fed with the high fat diet, the normal diet control group is further fed with the normal diet, the example 1 group, the example 2 group, the example 3 group, the example 4 group, the example 5 group and the example 6 group are respectively fed with 15g of the composition of each example each day, the inulin control group is fed with 15g of inulin each day, and the test period is 4 weeks.

1.2 detection method

1.2.1 obesity index assay

After the test was completed, the animal body weight was weighed with an electronic scale, and the body fat percentage was measured with an EchoMRI body composition analyzer.

1.2.2 Biochemical examination of blood

After the test is finished, blood of each group of test dogs is collected through the radial lateral vein on an empty stomach, and serum is obtained by centrifugation at 3000r/min for 10 min. The kit is used for measuring the contents of serum triglyceride, total cholesterol, high density lipoprotein cholesterol and low density lipoprotein cholesterol on the same day.

1.2.3 detection of intestinal flora

After the test is finished, fresh excrement discharged naturally is taken, each 100mg of excrement is weighed by a balance, and bacterial DNA of each group of excrement is extracted according to the instruction provided by the bacterial genome DNA extraction kit after the excrement is treated according to the experimental requirements. And respectively carrying out real-time fluorescence quantitative PCR reaction on DNA extracted from the fecal sample to be detected on lactobacillus, bifidobacterium and escherichia coli, analyzing the specificity of a PCR product according to a dissolution curve, and analyzing a quantitative result by a LightCycler PCR instrument.

1.3 data processing

All data were statistically analyzed using SPSS22.0 software.

2 results

2.1 Change in obesity index

The results are shown in fig. 2, and it can be seen that the body weight and body fat rate of animals in the high fat diet group are significantly higher than those in the normal diet group, indicating that the high fat diet successfully induces the canine obesity model. The compositions given in the different examples showed different reductions in body weight and body fat percentage, and example 1 showed better results than the inulin control group.

2.2 changes in Biochemical indicators of blood

As can be seen from Table 1, TG, TC and LDL-C in the blood of animals in the high-fat feed group are obviously increased and HDL-C is obviously reduced compared with those in the normal feed group, and the dry prognosis given to the composition of different examples can reduce the content of TG, TC and LDL-C in the blood and improve the content of HDL-C, and the effect of example 1 is better than that of the inulin control group. The composition has the function of reducing blood fat, and the effect is most obvious in example 1.

TABLE 1 Change in Biochemical index of blood

Group of	TG/(mmol/L)	TC/(mmol/L)	LDL-C/(mmol/L)	HDL-C/(mmol/L)
					Control group of normal feed	0.61±0.06*#	4.51±0.42*#	2.94±0.26*#	1.52±0.15*#
High fat feed control group	0.97±0.09	6.21±0.58	3.98±0.37	0.96±0.11
					Inulin control group	0.86±0.08*	5.55±0.52*	3.48±0.33*	1.13±0.10
EXAMPLE 1 group	0.72±0.07*#	4.98±0.49*#	3.15±0.31*	1.38±0.12*#
					EXAMPLE 2 group	0.85±0.08*	5.76±0.56	3.52±0.33	1.09±0.11
EXAMPLE 3 group	0.83±0.07*	5.64±0.55*	3.46±0.36	1.15±0.12*
					EXAMPLE 4 group	0.82±0.07*	5.57±0.50*	3.51±0.37*	1.17±0.13*
EXAMPLE 5 group	0.79±0.07*	5.41±0.51*	3.39±0.34*	1.23±0.12*
					EXAMPLE 6 group	0.77±0.07*	5.36±0.52*	3.37±0.36*	1.22±0.11*

Note: denotes P <0.05 compared to the high fat diet group and # denotes P <0.05 compared to the inulin control group.

2.3 variation of the number of intestinal flora

The results are shown in fig. 3, and it can be seen that high-fat diet induces the reduction of the quantity of bifidobacteria and lactobacilli, the increase of the quantity of escherichia coli and the reduction of the ratio of bifidobacteria to escherichia coli in intestinal tracts of dogs, after inulin is administered with the composition of each example, the quantity of bifidobacteria and lactobacilli is increased, the quantity of escherichia coli is reduced, the ratio of bifidobacteria to escherichia coli is increased, and the effect of the composition of example 1 is remarkably superior to that of an inulin control group, which shows that the composition has the effects of reducing weight and fat and regulating intestinal flora of obese dogs and cats, and has the dual effects of promoting the growth and reproduction of beneficial bacteria in intestinal tracts and inhibiting the growth and reproduction of harmful bacteria.

In conclusion, the composition with the effects of losing weight and reducing fat and regulating intestinal flora for obese dogs and cats has the advantages of homology of medicine and food, reduction of the defects of heavy taste of Chinese herbal medicines, low bioavailability and the like, is a product which is willing to be accepted by pets, enriches the weight-losing method for obese pets, can effectively reduce weight and body fat rate, regulates blood fat metabolism and intestinal flora, and is worthy of market popularization and application.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.