阅读说明：本技术 一种黑桃花色苷提取方法 (Extraction method of nectarine anthocyanin ) 是由 亓希武 梁呈元 陈泽群 房海灵 于盱 李莉 柏杨 刘冬梅 于 2021-08-26 设计创作，主要内容包括：本发明提供了一种黑桃花色苷提取方法,包括如下步骤：(1)黑桃果实烘干、粉碎、过筛；(2)低共熔溶剂提取：取一定量黑桃果实粉末,加入低共熔溶剂,振荡辅助提取,离心,得上清液即为花色苷提取液；(3)去除低共熔溶剂：利用大孔树脂去除低共熔溶剂,收集洗脱液；(4)干燥：旋蒸干燥洗脱液,得干燥粉末,即为黑桃花色苷提取物。该方法绿色、安全、高效,可得到高质量的黑桃花色苷。(The invention provides a method for extracting anthocyanin from black peaches, which comprises the following steps: (1) drying, crushing and sieving the black peach fruits; (2) eutectic solvent extraction: taking a certain amount of black peach fruit powder, adding a eutectic solvent, oscillating for auxiliary extraction, and centrifuging to obtain a supernatant, namely an anthocyanin extracting solution; (3) removing the eutectic solvent: removing the eutectic solvent by using macroporous resin, and collecting eluent; (4) and (3) drying: and (4) evaporating the dried eluent to dryness to obtain dried powder, namely the nectarine anthocyanin extract. The method is green, safe and efficient, and can obtain high-quality anthocyanin of fructus Persicae.)

1. A method for extracting anthocyanin from black peaches is characterized by comprising the following steps: the operation steps are as follows:

(1) drying, crushing and sieving the black peach fruits: cutting black peach fruits into blocks, drying in an oven, crushing and sieving;

(2) eutectic solvent extraction: taking a certain amount of black peach fruit powder, adding a eutectic solvent, oscillating for auxiliary extraction, and centrifuging to obtain a supernatant, namely an anthocyanin extracting solution;

(3) removing the eutectic solvent: removing the eutectic solvent by using macroporous resin, and collecting eluent;

(4) and (3) drying: and (4) evaporating the dried eluent to dryness to obtain dried powder, namely the nectarine anthocyanin extract.

2. The method for extracting anthocyanin from black peaches as claimed in claim 1, wherein the method comprises the following steps: in the step (1), the drying temperature is 40 ℃, and the mixture is sieved by a 60-mesh sieve.

3. The method for extracting anthocyanin from black peaches as claimed in claim 1, wherein the method comprises the following steps: in the step (2), the low eutectic solvent consists of choline chloride: oxalic acid =1:1 (molar ratio), water volume fraction is 30-35%, material-liquid ratio is 1: 20-1: 30, oscillation rate is 200rpm, extraction temperature is 40 ℃, extraction time is 30min, and centrifugation is carried out for 15min at 5000 rpm.

4. The method for extracting anthocyanin from black peaches as claimed in claim 1, wherein the method comprises the following steps: the macroporous resin used in the step (3) is macroporous resin X-5, and the eluent is 70% ethanol containing 0.1% hydrochloric acid.

5. The method for extracting anthocyanin from black peaches as claimed in claim 1, wherein the method comprises the following steps: in the step (4), the drying temperature is 50 ℃ by rotary evaporation.

Technical Field

The invention relates to the field of plant secondary metabolites, and particularly relates to a method for extracting anthocyanin from black peaches by using a eutectic solvent.

Background

Anthocyanin is a water-soluble pigment widely existing in plants, is mainly distributed in epidermal cells of flowers and fruits, and can make the flowers and fruits of the plants show various colors such as red, purple, blue and the like. Anthocyanins structurally belong to flavonoid compounds, and are plant secondary metabolites. Anthocyanins have various biological activities, including antiinflammatory, antioxidant, anticancer, free radical scavenging, and cardiovascular disease treating effects. In addition, anthocyanin is a safe and nontoxic natural pigment, and has important application in the aspects of food additives and health-care food.

The black peach is also named as a black peach, and the skin and the pulp of the black peach are black and purple, so that the black peach has high nutritional value. The black peach fruits contain rich anthocyanin and have high development value, so that the development of a safe, green and efficient anthocyanin extraction method has important significance for black peach anthocyanin development. The eutectic solvent is a deep crystal mixture consisting of a Hydrogen Bond Acceptor (HBA) and a Hydrogen Bond Donor (HBD), is a green solvent, and is an effective substitute of the traditional extraction technology. The eutectic solvent can improve the stability of the extract and has great application potential in the extraction of various natural products.

Disclosure of Invention

The invention aims to provide a method for extracting anthocyanin from black peaches, which can be realized by the following technical scheme:

(1) drying, crushing and sieving the black peach fruits: cutting black peach fruits into blocks, drying in a drying oven at 40 ℃, crushing, and sieving by a 60-mesh sieve;

(2) eutectic solvent extraction: taking a certain amount of black peach fruit powder, and adding a eutectic solvent according to a material-liquid ratio of 1: 20-1: 30, wherein the eutectic solvent comprises choline chloride: oxalic acid =1:1 (molar ratio), the volume fraction of water is 30-35%, the extraction is assisted by oscillation at 200rpm, the extraction temperature is 40 ℃, the extraction time is 30min, and the extraction is carried out for 15min at 5000rpm, so that the supernatant is the anthocyanin extracting solution;

(3) removing the eutectic solvent: loading the anthocyanin extracting solution into a macroporous resin X-5 chromatographic column, washing with deionized water until the pH value is neutral, then eluting with 70% ethanol containing 0.1% hydrochloric acid, and collecting the eluent;

(4) and (3) drying: and (4) carrying out rotary evaporation on the eluent at 50 ℃ to obtain dry powder, namely the nectarine anthocyanin extract.

Detailed Description

In order that those skilled in the art will better understand the technical solutions of the present invention, the following detailed description of the present invention is provided in connection with specific embodiments.

Example 1

A method for extracting anthocyanin from black peaches comprises the following steps:

(1) cutting the black peach fruits into blocks, drying the black peach fruits in a drying oven at 40 ℃, crushing the black peach fruits by using a crusher, and sieving the black peach fruits by using a 60-mesh sieve;

(2) weighing 5g of black peach fruit powder, and adding 100mL of eutectic solvent according to the ratio of 1:20, wherein the eutectic solvent comprises choline chloride: oxalic acid =1:1 (molar ratio), water volume fraction is 30%, oscillation extraction is carried out at 200rpm under 40 ℃ for 30min, and then centrifugation is carried out at 5000rpm for 15min, so as to obtain supernatant;

(3) loading the supernatant into a macroporous resin X-5 chromatographic column, washing with deionized water until the pH is neutral, eluting with 200mL 70% ethanol containing 0.1% hydrochloric acid, and collecting the eluate;

(4) and (4) carrying out rotary evaporation on the eluent at 50 ℃ to obtain dry powder, namely the nectarine anthocyanin extract.