阅读说明：本技术 一种缸体活塞组件及色谱柱 (Cylinder body piston assembly and chromatographic column ) 是由 肖立峰 孙新芬 于 2021-09-18 设计创作，主要内容包括：本发明公开了一种缸体活塞组件,包括刚性的缸体,还包括刚性的内衬套及设于内衬套外周的弹性的密封套,所述内衬套的圆周面上设有用于容纳所述密封套受压后的变形量的容纳部,所述缸体设于所述密封套的外周。本发明进一步公开了一种色谱柱,包括上述的缸体活塞组件,所述缸体形成色谱柱的柱管,所述内衬套上侧形成压力溶剂腔,内衬套下侧形成填料柱床腔。本发明具有结构简单、成本低,有利于防止串液,可重复使用等优点。(The invention discloses a cylinder body piston assembly, which comprises a rigid cylinder body, a rigid inner bushing and an elastic sealing sleeve arranged on the periphery of the inner bushing, wherein a containing part used for containing the deformation of the compressed sealing sleeve is arranged on the circumferential surface of the inner bushing, and the cylinder body is arranged on the periphery of the sealing sleeve. The invention further discloses a chromatographic column which comprises the cylinder piston assembly, wherein the cylinder forms a column tube of the chromatographic column, the upper side of the inner bushing forms a pressure solvent cavity, and the lower side of the inner bushing forms a packed column bed cavity. The invention has the advantages of simple structure, low cost, easy prevention of liquid mixing, repeated use and the like.)

1. A cylinder-piston assembly comprising a rigid cylinder (3), characterized in that: the cylinder body is characterized by further comprising a rigid inner bushing (1) and an elastic sealing sleeve (2) arranged on the periphery of the inner bushing (1), wherein a containing portion (11) used for containing the deformation of the sealing sleeve (2) after being pressed is arranged on the circumferential surface of the inner bushing (1), and the cylinder body (3) is arranged on the periphery of the sealing sleeve (2).

2. The cylinder-piston assembly of claim 1, wherein: the accommodating portion (11) is provided in plurality and arranged at intervals in the axial direction of the cylinder body (3).

3. The cylinder-piston assembly of claim 2, wherein: the accommodating part (11) is a ring groove arranged along the circumferential direction of the inner bushing (1).

4. The cylinder-piston assembly of claim 3, wherein: the annular grooves are arc-shaped grooves, and an arc transition part (12) is arranged between every two adjacent arc-shaped grooves.

5. The cylinder-piston assembly according to any one of claims 1 to 4, wherein: the upper end of the inner bushing (1) is provided with a limiting flange (13), the upper end of the sealing sleeve (2) is abutted to the limiting flange (13), and the lower end of the sealing sleeve (2) is in threaded connection with the lower end of the inner bushing (1).

6. The cylinder-piston assembly of claim 5, wherein: the upper end face of the inner bushing (1) is provided with a first liquid inlet connector (42), the lower end face of the inner bushing is provided with a uniform flow groove (14), a uniform flow screen plate (5) is arranged below the inner bushing (1), the inner side of the sealing sleeve (2) is provided with a limiting step (21), and the uniform flow screen plate (5) is abutted to the limiting step (21).

7. A chromatography column, characterized by: comprising a cylinder-piston assembly according to any one of claims 1 to 6, the cylinder (3) forming the column tube of a chromatography column, the upper side of the inner liner (1) forming a pressurized solvent chamber (31), the lower side of the inner liner (1) forming a packed bed chamber (32).

8. The chromatography column of claim 7, wherein: the upper end of the column tube is provided with a second liquid inlet joint (41), and a telescopic pipeline (43) is connected between the second liquid inlet joint (41) and the first liquid inlet joint (42).

9. The chromatography column of claim 8, wherein: the lower end of the column tube is provided with a lower flange (33), and a detachable column tube lower cover (34) is arranged on the lower flange (33).

10. The chromatography column of claim 9, wherein: the lower flange (33) is in threaded fit with the column pipe, and the column pipe lower cover (34) is mounted on the lower flange (33) through a fastener (35).

Technical Field

The invention relates to separation and purification equipment, in particular to a cylinder piston assembly and a chromatographic column.

Background

With the rapid development of biological medicine and chemical industry, preparative liquid chromatography has been widely applied in the field of separation and purification, wherein the dynamic axial compression technology is one of the important technologies in the field of preparative chromatography. In dynamic axial compression chromatography columns, the sealing effect of the piston directly affects the purification effect of the column. At present, the common dynamic axial compression chromatographic column piston in the market basically realizes the high-pressure sealing of a column bed in a chromatographic column by a spring energy storage high-pressure sealing ring, the upper side and the lower side of the spring energy storage high-pressure sealing ring are respectively provided with a guide sleeve, but the piston of the structure can only be pressed at one side, when a pressure solvent is used for driving the piston to move up and down, and the upper side and the lower side of the piston are required to bear the high-pressure solvent, the sealing effect cannot be achieved, the phenomena of liquid mixing and liquid leakage can occur, and the piston assembly of the structure has higher cost.

Disclosure of Invention

The invention aims to solve the technical problem of overcoming the defects of the prior art and provides a cylinder piston assembly which is simple in structure, low in cost, beneficial to preventing liquid leakage and capable of being repeatedly used.

The invention further provides a chromatographic column comprising the cylinder-piston assembly.

In order to solve the technical problems, the invention adopts the following technical scheme:

a cylinder body piston assembly comprises a rigid cylinder body, a rigid inner bushing and an elastic sealing sleeve arranged on the periphery of the inner bushing, wherein a containing portion used for containing the deformation of the compressed sealing sleeve is arranged on the circumferential surface of the inner bushing, and the cylinder body is arranged on the periphery of the sealing sleeve.

As a further improvement of the above technical solution:

the accommodating portion is provided in plurality and arranged at intervals in an axial direction of the cylinder body.

The accommodating part is a ring groove arranged along the circumferential direction of the inner bushing.

The annular groove is an arc-shaped groove, and an arc transition part is arranged between every two adjacent arc-shaped grooves.

The inner bushing is a steel inner bushing, and the sealing sleeve is a polytetrafluoroethylene or modified polytetrafluoroethylene sealing sleeve.

The upper end of the lining sleeve is provided with a limiting flange, the upper end of the sealing sleeve is abutted to the limiting flange, and the lower end of the sealing sleeve is in threaded connection with the lower end of the lining sleeve.

The inner liner upper end face is equipped with first inlet joint, and the lower terminal surface is equipped with even chute, and the inner liner below is equipped with even flow otter board, the seal cover inboard is equipped with spacing step, even flow otter board with spacing step butt.

A chromatographic column comprises the cylinder body piston assembly, the cylinder body forms a column tube of the chromatographic column, a pressure solvent cavity is formed on the upper side of an inner bushing, and a packed column bed cavity is formed on the lower side of the inner bushing.

As a further improvement of the above technical solution:

the upper end of the column tube is provided with a second liquid inlet joint, and a telescopic pipeline is connected between the first liquid inlet joint and the second liquid inlet joint.

The lower end of the column tube is provided with a lower flange, and a detachable column tube lower cover is arranged on the lower flange.

The lower flange is in threaded fit with the column pipe, and the column pipe lower cover is mounted on the lower flange through a fastener.

Compared with the prior art, the invention has the advantages that: the cylinder piston assembly disclosed by the invention has the advantages that the rigid inner bushing is arranged on the inner side of the sealing sleeve, the rigid cylinder body is arranged on the outer side of the sealing sleeve, the accommodating part is arranged on the circumferential surface of the inner bushing, when the two ends of the sealing sleeve are extruded to generate deformation, the deformation on the outer side is limited by the cylinder body, and the deformation on the inner side is filled into the accommodating part, so that a good sealing effect is realized, the liquid on the two ends can be prevented from being mixed with each other, the pressure on the two ends is higher, the sealing sleeve is more tightly attached to the accommodating part, the structure is simple and reliable, and the extruded part of the sealing sleeve can be gradually rebounded after the inner bushing and the sealing sleeve are disassembled from the cylinder body, so that the repeated use is realized.

The chromatographic column disclosed by the invention comprises the cylinder body piston assembly, the cylinder body is used as a column tube, the upper side and the lower side of the inner bushing are respectively provided with the pressure solvent cavity and the packed column bed cavity, and the seal sleeve can play a good sealing role under the working pressure of the pressure solvent cavity and the packed column bed cavity so as to prevent the mutual liquid leakage between the cavities.

Drawings

Fig. 1 is a schematic structural diagram of a cylinder-piston assembly of the present invention.

Fig. 2 is a partially enlarged view of fig. 1.

FIG. 3 is a schematic diagram of the structure of a chromatography column of the present invention.

Fig. 4 is a partially enlarged view of fig. 3.

The reference numerals in the figures denote: 1. an inner liner; 11. an accommodating portion; 12. a circular arc transition portion; 13. a limiting flange; 14. a uniform flow groove; 2. sealing sleeves; 21. a limiting step; 3. a column tube; 31. a pressurized solvent chamber; 32. a packed column bed chamber; 33. a lower flange; 34. a lower cover of the column tube; 35. a fastener; 36. an upper flange; 37. a column tube upper cover; 41. a second liquid inlet joint; 42. a first liquid inlet joint; 43. a telescopic pipeline; 5. and (4) uniformly flowing screen plates.

Detailed Description

The invention is described in further detail below with reference to the figures and specific examples of the specification.

Fig. 1 to 2 show an embodiment of the cylinder piston assembly according to the present invention, which includes a rigid cylinder 3, a rigid inner liner 1, and an elastic gland 2 disposed on an outer periphery of the inner liner 1, wherein a receiving portion 11 for receiving a deformation amount of the gland 2 after being pressed is disposed on a circumferential surface of the inner liner 1, and the cylinder 3 is disposed on an outer periphery of the gland 2.

This cylinder body piston assembly, 2 inboard rigid neck bush 1 that are of seal cover, the outside is rigid cylinder body 3, be equipped with accommodating part 11 on the periphery of neck bush 1, when 2 both ends of seal cover receive the extrusion to produce when warping, outside deformation is restricted by cylinder body 3, inside deflection then is filled to accommodating part 11 in, thereby play good sealed effect, can prevent the mutual liquid of cross liquid at both ends, and the pressure at both ends is big more, seal cover 2 is tighter with the laminating of accommodating part 11, moreover, the steam generator is simple in structure, reliability, after neck bush 1 and seal cover 2 are dismantled from cylinder body 1, seal cover 2 receives extruded part to kick-back gradually, thereby realize used repeatedly.

Further, in the present embodiment, the accommodating portion 11 is provided in plurality and arranged at intervals in the axial direction of the cylinder 3, so that a plurality of stages of mutually-spaced seals can be formed in the axial direction of the cylinder 3, and when a certain stage of seal fails, the sealing performance of the assembly as a whole can still be ensured, and the reliability is higher.

As a preferred embodiment, the receiving portion 11 is a ring groove arranged along the circumferential direction of the inner liner 1, and the sealing sleeve 2 is deformed and filled into the ring groove along the entire circumferential direction, so that a seal can be formed along the entire circumferential direction.

Furthermore, in this embodiment, the ring grooves are arc-shaped grooves, and the arc transition portion 12 is disposed between two adjacent arc-shaped grooves, so that the arc-shaped grooves and the arc transition portion 12 are favorable for the sealing sleeve 2 to closely adhere to the circumferential surface of the inner bushing 1 after being deformed by pressure, and a gap is avoided.

As a preferred embodiment, the inner bushing 1 may be a steel inner bushing, the cylinder 3 may be a steel cylinder, and the sealing sleeve 2 is a teflon sealing sleeve or a modified teflon sealing sleeve, which is easy to deform, good in elasticity, and long in service life.

In this embodiment, the upper end of the inner liner 1 is provided with a limit flange 13, the upper end of the sealing sleeve 2 abuts against the limit flange 13, the lower end of the sealing sleeve 2 is in threaded connection with the lower end of the inner liner 1, further, the upper end surface of the inner liner 1 is provided with a first liquid inlet joint 42, the lower end surface is provided with a uniform flow groove 14, the lower side of the inner liner 1 is provided with a uniform flow screen plate 5, the inner side of the sealing sleeve 2 is provided with a limit step 21, and the uniform flow screen plate 5 abuts against the limit step 21. The upper end of the sealing sleeve 2 is abutted against a limiting flange 13 on the inner bushing 1, the lower end of the sealing sleeve 2 is in threaded connection with the lower end of the inner bushing 1, and the uniform flow screen plate 5 is clamped between the inner bushing 1 and a limiting step 21, so that the inner bushing 1, the sealing sleeve 2 and the uniform flow screen plate 5 can be effectively connected into a whole and synchronously move up and down in the cylinder body 3, the relative displacement among the sealing sleeve 2, the uniform flow screen plate 5 and the inner bushing 1 is prevented, on the other hand, the upper end of the sealing sleeve 2 can deform after being extruded, the sealing performance is guaranteed, and the structure is reasonable and reliable.

Fig. 3 to 4 show an embodiment of the chromatography column of the present invention, which includes the above-mentioned cylinder piston assembly, wherein the cylinder 3 forms the column tube of the chromatography column, the upper side of the inner sleeve 1 forms a pressure solvent chamber 31, and the lower side of the inner sleeve 1 forms a packed bed chamber 32.

The chromatographic column comprises the cylinder piston assembly, the cylinder 3 is used as a column tube, the upper side and the lower side of the inner bushing 1 are respectively provided with a pressure solvent cavity 31 and a packed column bed cavity 32, and the sealing sleeve can play a good sealing role under the working pressure of the pressure solvent cavity 31 and the packed column bed cavity 32, so that liquid cross connection between the cavities is prevented.

The chromatographic column comprises the cylinder body piston assembly, the cylinder body 3 is used as a column tube, the upper side and the lower side of the inner bushing 1 are respectively provided with a pressure solvent cavity 31 and a packed column bed cavity 32, and the sealing sleeve 2 can play a good sealing role under the working pressure of the pressure solvent cavity 31 and the packed column bed cavity 32 so as to prevent the cavities from being mixed with each other.

Further, in this embodiment, a second liquid inlet joint 41 is provided at the upper end of the column tube, and an expansion pipe 43 is connected between the first liquid inlet joint 41 and the second liquid inlet joint 42. The sample can sequentially enter the inner bushing 1 through the second liquid inlet joint 41, the telescopic pipeline 43 and the first liquid inlet joint 42, then uniformly disperse in all directions through the uniform flow groove 14, and finally uniformly enter the packed column bed cavity 32 through the uniform flow screen plate 5, and the telescopic pipeline 43 stretches along with the up-and-down movement of the inner bushing 1 and the sealing sleeve 2 in the column tube, so that the structure is simple and reliable.

Further, in this embodiment, the lower end of the column tube is provided with a lower flange 33, preferably, the lower flange 33 is connected with the lower end of the column tube through a screw thread, and the lower flange 33 is provided with a detachable lower column tube cover 34 (for example, a fastener, a snap, etc. are used to realize detachable connection). After the lower cover 34 of the column tube is disassembled, the pressure solvent is introduced into the pressure solvent cavity 31, the inner bushing 1 and the sealing sleeve 2 integrally move downwards under the pressure action of the pressure solvent to push the packed column bed out of the column tube, so that the column is disassembled without manual participation, and secondary pollution is avoided.

Although the present invention has been described with reference to the preferred embodiments, it is not intended to be limited thereto. Those skilled in the art can make numerous possible variations and modifications to the present invention, or modify equivalent embodiments to equivalent variations, without departing from the scope of the invention, using the teachings disclosed above. Therefore, any simple modification, equivalent change and modification made to the above embodiments according to the technical spirit of the present invention should fall within the protection scope of the technical scheme of the present invention, unless the technical spirit of the present invention departs from the content of the technical scheme of the present invention.