阅读说明：本技术 一种自搭建变焦显微图像拍摄系统及方法 (Self-built zoom microscopic image shooting system and method ) 是由 何勇 张泽清 肖舒裴 方慧 于 2020-05-18 设计创作，主要内容包括：本发明涉及一种自搭建变焦显微图像拍摄系统及方法。所述方法包括光学实验平台、相机、显微镜和微动载物平台；所述微动载物平台固定在所述光学实验平台的底座上；所述显微镜设置相机与所述微动载物平台之间,且所述显微镜固定在所述光学实验平台的夹持装置上；所述相机设置在所述显微镜的目镜处,所述相机的光学中心与所述显微镜的轴心处于同一条垂线；所述微动载物平台用于调整所述相机聚焦在拍摄对象表面的高度位置。本发明所提供的一种自搭建变焦显微图像拍摄系统及方法提高对拍摄对象三维形貌拍摄的精确度。(The invention relates to a self-built zoom microscopic image shooting system and a self-built zoom microscopic image shooting method. The method comprises an optical experiment platform, a camera, a microscope and a micro-motion carrying platform; the micro-motion carrying platform is fixed on a base of the optical experiment platform; the microscope is arranged between the camera and the micro-motion carrying platform and is fixed on the clamping device of the optical experiment platform; the camera is arranged at an eyepiece of the microscope, and the optical center of the camera and the axis of the microscope are in the same vertical line; the micro-motion object carrying platform is used for adjusting the height position of the camera focused on the surface of the shooting object. The self-built zoom microscopic image shooting system and method provided by the invention improve the shooting accuracy of the three-dimensional shape of the shot object.)

1. A self-built zoom microscopic image shooting system is characterized by comprising: the system comprises an optical experiment platform, a camera, a microscope and a micro-motion carrying platform;

the micro-motion carrying platform is fixed on a base of the optical experiment platform; the microscope is arranged between the camera and the micro-motion carrying platform and is fixed on the clamping device of the optical experiment platform; the camera is arranged at an eyepiece of the microscope, and the optical center of the camera and the axis of the microscope are in the same vertical line;

the micro-motion object carrying platform is used for adjusting the height position of the camera focused on the surface of the shooting object.

2. The self-built zoom microscopic image capturing system according to claim 1, wherein the micro-motion stage comprises a coarse adjustment knob and a fine adjustment knob.

3. The self-built zoom microscopic image photographing system according to claim 1, further comprising: a light supplement lamp;

the light supplement lamp is arranged on the micro-motion carrying platform; and an objective lens of the microscope penetrates through a hole at the top end of the light supplement lamp.

4. The self-built zoom microscopic image photographing system according to claim 3, wherein the fill-in light is an annular fill-in light.

5. The self-built zoom microscopic image photographing system according to claim 1, further comprising: a computer;

the computer is connected with the camera through the Ethernet; the computer is used for determining the microstructure of the surface of the shooting object according to the microscopic image shot by the camera.

6. The self-building zoom microscopic image photographing system according to claim 1, wherein the camera is an industrial camera.

7. The self-built zoom microscope image photographing system according to claim 1, wherein the microscope is a portable microscope.

8. A self-built zoom microscopic image shooting method is applied to the self-built zoom microscopic image shooting system of any one of claims 1 to 7, and comprises the following steps:

rotating a coarse adjustment knob and a fine adjustment knob of the micro-motion carrying platform to adjust the position of a shooting object on the micro-motion carrying platform;

shooting a shooting object on the micro-motion carrying platform by using a camera to obtain focused images at different focusing positions;

and determining the microstructure of the surface of the shot object by adopting a focusing shape recovery method for all focused images.

9. The method for shooting the self-built zoom microscopic image according to claim 8, wherein the coarse adjustment knob and the fine adjustment knob of the micro-motion object platform are rotated to adjust the position of the shot object on the micro-motion object platform, and the method further comprises the following steps:

and rotating a knob on the clamping device to enable the objective lens of the microscope to penetrate through the hole at the top end of the light supplementing lamp.

Technical Field

The invention relates to the field of image shooting, in particular to a self-built zoom microscopic image shooting system and a self-built zoom microscopic image shooting method.

Background

The three-dimensional photomicrography is widely applied to the fields of life science, material science and the like. In these fields, three-dimensional photomicrographic instruments commonly used mainly include scanning electron microscopes, three-dimensional profilometers, atomic force microscopes, and the like. An image obtained by a scanning electron microscope is a two-dimensional image, and a related scholars regard the gray scale change of the image as the surface appearance change of a sample, but the two-dimensional gray scale information cannot truly reflect three-dimensional information. Scanning electron microscopy also requires complex pre-processing of the sample. The atomic force microscope measures the surface of a sample by moving a probe, the measurement process is one-dimensional, and the contact type probe may damage the surface of the sample. The three-dimensional profilometer utilizes white light interference to obtain a three-dimensional surface, which is less accurate than scanning electron microscopes and atomic force microscopes. In addition, these specialized microscopic instruments have the common disadvantages of being expensive and having a low depth of field. The depth of field problem severely limits the surface topography of the object it captures. For example, the microstructure of the surface of a plant leaf is an important factor influencing the free energy of the surface of the leaf and is closely related to the deposition effect of a liquid medicine, while the surface of the plant leaf is usually a multi-scale fractal structure, and a microscopic instrument with low depth of field cannot completely observe and photograph the structure. At such a scale, the common three-dimensional imaging instrument (such as a laser radar) cannot meet the requirement on precision.

Disclosure of Invention

The invention aims to provide a self-built zoom microscopic image shooting system and a self-built zoom microscopic image shooting method, which are used for improving the shooting accuracy of the three-dimensional shape of a shot object.

In order to achieve the purpose, the invention provides the following scheme:

a self-built zoom microscopy image capture system, comprising: the system comprises an optical experiment platform, a camera, a microscope and a micro-motion carrying platform;

the micro-motion carrying platform is fixed on a base of the optical experiment platform; the microscope is arranged between the camera and the micro-motion carrying platform and is fixed on the clamping device of the optical experiment platform; the camera is arranged at an eyepiece of the microscope, and the optical center of the camera and the axis of the microscope are in the same vertical line;

the micro-motion object carrying platform is used for adjusting the height position of the camera focused on the surface of the shooting object.

Optionally, the micro carrier platform includes a coarse adjustment knob and a fine adjustment knob.

Optionally, the self-built zoom microscopic image shooting system further includes: a light supplement lamp;

the light supplement lamp is arranged on the micro-motion carrying platform; and an objective lens of the microscope penetrates through a hole at the top end of the light supplement lamp.

Optionally, the light supplement lamp is an annular light supplement lamp.

Optionally, the self-built zoom microscopic image shooting system further includes: a computer;

the computer is connected with the camera through the Ethernet; the computer is used for determining the microstructure of the surface of the shooting object according to the microscopic image shot by the camera.

Optionally, the camera is an industrial camera.

Optionally, the microscope is a portable microscope.

A self-built zoom microscopic image shooting method is applied to a self-built zoom microscopic image shooting system, and comprises the following steps:

rotating a coarse adjustment knob and a fine adjustment knob of the micro-motion carrying platform to adjust the position of a shooting object on the micro-motion carrying platform;

shooting a shooting object on the micro-motion carrying platform by using a camera to obtain focused images at different focusing positions;

and determining the microstructure of the surface of the shot object by adopting a focusing shape recovery method for all focused images.

Optionally, the step of rotating the coarse adjustment knob and the fine adjustment knob of the fine movement object stage to adjust the position of the object to be photographed on the fine movement object stage further includes:

and rotating a knob on the clamping device to enable the objective lens of the microscope to penetrate through the hole at the top end of the light supplementing lamp.

According to the specific embodiment provided by the invention, the invention discloses the following technical effects:

the invention provides a self-built zoom microscopic image shooting system and a self-built zoom microscopic image shooting method. When shooting is carried out, the space focusing position information of the shot image sequence can be obtained, so that the microscopic surface three-dimensional appearance of the shot object is calculated, and the shooting accuracy of the three-dimensional appearance of the shot object is improved.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.

Fig. 1 is a schematic structural diagram of a self-built zoom microscopic image shooting system provided by the invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention aims to provide a self-built zoom microscopic image shooting system and a self-built zoom microscopic image shooting method, which are used for improving the shooting accuracy of the three-dimensional shape of a shot object.

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.

Fig. 1 is a schematic structural diagram of a self-built zoom microscopic image photographing system provided by the present invention, the self-built zoom microscopic image photographing system includes: an optical experiment platform 1, a camera 4, a microscope 3 and a micro-motion object stage 2. The camera 4 is an industrial camera; the microscope 3 is a portable microscope.

The micro-motion carrying platform 2 is fixed on a base of the optical experiment platform 1; the microscope 3 is arranged between the camera 4 and the micro-motion object carrying platform 2, and the microscope 3 is fixed on a clamping device of the optical experiment platform 1; the camera 4 is arranged at an eyepiece of the microscope 3, and an optical center of the camera 4 and an axis of the microscope 3 are in the same vertical line.

The micro-motion loading platform 2 is used for adjusting the height position of the camera 4 focused on the surface of the shooting object.

The micro-motion object carrying platform 2 comprises a coarse adjustment knob and a fine adjustment knob.

The self-built zoom microscopic image shooting system further comprises: a light supplement lamp 5; the light supplement lamp 5 is specifically an annular light supplement lamp.

The light supplement lamp 5 is arranged on the micro-motion carrying platform 2; and an objective lens of the microscope 3 penetrates through a hole in the top end of the light supplement lamp 5. The light supplement lamp 5 is arranged on the micro-motion carrying platform 2 and covers the whole shooting object, and is used for adjusting the brightness of a view field.

The self-built zoom microscopic image shooting system further comprises: and a computer 6.

The computer 6 is connected with the camera 4 through Ethernet; the computer 6 is used for determining the microstructure of the surface of the shooting object according to the microscopic image shot by the camera 4.

As a specific embodiment, the self-built zoom microscopic image shooting system provided by the invention has the specific operation process that:

and S1, rotating the coarse adjustment knob of the micro-motion carrying platform 2 anticlockwise to the maximum reading value, and rotating the fine adjustment knob of the micro-motion carrying platform 2 clockwise to the minimum reading value.

And S2, flatly placing the shot object on the micro-motion carrying platform 2, and covering the annular light supplement lamp 5. The knob of the optical experiment platform 1 is rotated, so that the portable microscope 3 downwards penetrates through the hole in the top end of the annular light supplement lamp 5, and the distance between the lens of the microscope 3 and the micro-motion carrying platform 2 is about 0.5 cm.

And S3, turning on image acquisition software on the computer 6, and adjusting the annular light supplement lamp 5 to a proper light supplement intensity.

And S4, clockwise rotating the coarse adjustment knob of the micro-motion carrying platform 2, and moving the carrying platform downwards until all points on the image are observed to pass through the process of 'fuzzy-clear-fuzzy', and acquiring the current image.

S5, the fine adjustment knob of the micro stage 2 is rotated counterclockwise by a small grid (depending on the required resolution of the depth information), and the stage will move up by 1 μm. A current image is acquired.

S6, repeating S5Until all points on the image are observed to go through the process of "blur-sharpness-blur" again.

According to the self-built zoom microscopic image shooting system provided by the invention, the parts of the system are provided with mature and cheap products on the market, and the price of the whole system is far lower than that of a currently common large instrument; the surface of the leaf is directly shot by utilizing an optical principle without an additional pretreatment process; each part of the system is fixed by adopting a knob and a nut, and is easy to disassemble and assemble. The whole system is light in weight, and can be loaded into a suitcase to be carried to a scene for shooting.

The invention also provides a self-built zoom microscopic image shooting method, the self-built zoom microscopic image shooting method corresponds to the self-built zoom microscopic image shooting system provided by the invention, and the self-built zoom microscopic image shooting method comprises the following steps:

and S201, rotating the coarse adjustment knob and the fine adjustment knob of the micro-motion carrying platform 2 to adjust the position of the shooting object on the micro-motion carrying platform 2.

Before S201, in order to improve the shooting effect, the light supplement lamp 5 is turned on, and the knob on the clamping device is rotated, so that the objective lens of the microscope 3 passes through the hole at the top end of the light supplement lamp 5.

S202, shooting the shooting object on the micro-motion loading platform 2 by using the camera 4 to obtain focused images at different focusing positions.

And S203, determining the microstructure of the surface of the shot object by adopting a focus recovery shape method for all the focused images.

The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. For the system disclosed by the embodiment, the description is relatively simple because the system corresponds to the method disclosed by the embodiment, and the relevant points can be referred to the method part for description.

The principles and embodiments of the present invention have been described herein using specific examples, which are provided only to help understand the method and the core concept of the present invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.