阅读说明：本技术 一种瞿麦中tunicoside B的分离制备方法 (Method for separating and preparing tunicoside B in fringed pink ) 是由 李刚 袁晨 吕悦 王振华 于 2021-09-18 设计创作，主要内容包括：本发明涉及天然药物化学技术领域,具体涉及一种瞿麦中麦芽酚苷类化合物tunicoside B的分离制备方法。其制备方法：提取、微孔树脂中压色谱粗分、反相中压色谱富集及反相/亲水高压二维液相色谱纯化四个步骤。本发明成本低廉、产品纯度大于98%；本发明采用的技术手段可进行规模化生产：原料要求不高、成本低廉、极易获取,易于批量备料；乙醇室温冷浸提取,易于操作；分离采用微孔树脂中压色谱粗分、反相中压色谱富集,涉及的两种分离材料可以装于中压柱层析系统中,易于规模化；分离纯化中使用的反相制备液相色谱或亲水制备液相色谱,为快速的等度方法。(The invention relates to the technical field of natural pharmaceutical chemistry, in particular to a separation and preparation method of a maltoside compound, namely, tunicoside B in dianthus superbus. The preparation method comprises the following steps: the method comprises four steps of extraction, microporous resin medium pressure chromatography rough separation, reversed phase medium pressure chromatography enrichment and reversed phase/hydrophilic high pressure two-dimensional liquid chromatography purification. The invention has low cost and the product purity is more than 98 percent; the technical means adopted by the invention can be used for large-scale production: the raw materials have low requirements, low cost and easy acquisition, and are easy to prepare in batches; the ethanol is extracted by cold soaking at room temperature, and the operation is easy; the separation adopts microporous resin medium-pressure chromatography rough separation and reversed-phase medium-pressure chromatography enrichment, and the two related separation materials can be arranged in a medium-pressure column chromatography system and are easy to scale; the reversed-phase preparative liquid chromatography or hydrophilic preparative liquid chromatography used in the separation and purification is a rapid isocratic method.)

1. A method for separating and preparing a maltoside compound, namely, tunicoside B in dianthus superbus, is characterized by comprising the following steps: the process specifically comprises the following steps:

step 1, extraction: drying the fringed pink medicinal material in the shade, coarsely crushing the fringed pink medicinal material, and mixing the raw materials according to a material-liquid ratio of 1 g: extracting with 5-100 mL of ethanol at room temperature for 2-4 times, each time for 2-4 hours, filtering, and combining the filtrates to obtain a filtrate A, wherein the amount of the silica gel in the filtrate A is as follows: the weight of the dianthus superbus medicinal materials =1: 5-15 mixing the sample and drying under reduced pressure to obtain a mixed sample of the dianthus superbus extract;

step 2, carrying out medium-pressure chromatography rough separation on microporous resin: mixing the Dianthus superbus extract with sample, subjecting the sample to medium pressure chromatographic separation with microporous resin, detecting with ultraviolet detector with detection wavelength of 210 nm, collecting the fourth chromatographic peak fraction in the preparative chromatogram, and drying under reduced pressure to obtain target component Fr 4;

and 3, enriching by reverse phase medium pressure chromatography: mixing the target component Fr4 with silica gel to obtain a sample, separating the sample by a reverse phase medium pressure chromatographic column filled with silica gel matrix material, detecting by an ultraviolet detector with the detection wavelength of 210 nm, collecting the fraction of the fourth chromatographic peak in the preparative chromatogram, and drying the fraction under reduced pressure to obtain a component Fr4-4 containing the target component;

and 4, purifying by reversed phase/hydrophilic high-pressure two-dimensional liquid chromatography: dissolving a component Fr4-4 containing a target component in a methanol-water solution with the volume fraction of 50-100%, preparing a sample with the concentration of 20.0-50.0 mg/mL, filtering with a 0.45-micrometer microporous filter membrane to obtain a filtrate, namely a filtrate B, separating the filtrate B by reverse-phase high-pressure liquid chromatography, detecting by an ultraviolet detector with the detection wavelength of 210 nm, collecting a first chromatographic peak fraction in a reverse-phase high-pressure preparation chromatogram of the filtrate B, and drying under reduced pressure to obtain a component Fr441 containing the target component; dissolving a component Fr441 containing a target component by using a methanol solution with the volume fraction of 100%, preparing a sample with the concentration of 25.0 mg/mL, filtering the sample by using a 0.45-micrometer microporous filter membrane to obtain a filtrate C, purifying the filtrate C by using hydrophilic high-pressure liquid chromatography, detecting the purified filtrate C by using an ultraviolet detector with the detection wavelength of 210 nm, collecting a first chromatographic peak fraction Fr4411 in a hydrophilic high-pressure preparation chromatogram of the filtrate C, and drying the chromatographic peak fraction under reduced pressure to obtain a tunicoside B chemical reference substance with the purity of more than 98%.

2. The method for separating and preparing the maltoside B compound from Dianthus superbus according to claim 1, wherein the maltoside B compound is selected from the group consisting of: in the step 1, the step 2, the step 3 and the step 4, the conditions of reduced pressure drying are as follows: the vacuum degree is 50-250 mbar, and the temperature is 40-60 ℃.

3. The method for separating and preparing the maltoside B compound from Dianthus superbus according to claim 1, wherein the maltoside B compound is selected from the group consisting of: in the step 2, the separation working parameters of the pressure chromatography rough separation in the microporous resin are as follows: the length of a chromatographic column is 460 mm, the diameter of the chromatographic column is 49 mm, the stationary phase of a microporous resin column is HP20SS, the mobile phase A is water, the mobile phase B is methanol, the mobile phase C is dichloromethane, the chromatographic conditions are 0-20 min, 0% B, 20-240 min, 0% -100% B, 240-300 min, 100% B, 300-420 min and 0-100% C, the sample injection amount is 10-80 g, and the flow rate is 40-60 mL/min.

4. The method for separating and preparing the maltoside B compound from Dianthus superbus according to claim 1, wherein the maltoside B compound is selected from the group consisting of: in the step 3, the working parameters of the reversed phase medium pressure chromatographic enrichment are as follows: the length of a chromatographic column is 500 mm, the diameter of the chromatographic column is 50 mm, the stationary phase of a reversed-phase preparation column is Spherical C18 with the diameter of 50 microns, a mobile phase A is water, a mobile phase B is methanol, the chromatographic condition is 0-120 min, the concentration of B is 20-65%, the sample injection amount is 10-30 g, and the flow rate is 40-60 mL/min.

5. The method for separating and preparing the maltoside B compound from Dianthus superbus according to claim 1, wherein the maltoside B compound is selected from the group consisting of: in the step 4, the working parameters of the reversed phase/hydrophilic high-pressure two-dimensional liquid chromatography purification are as follows: the chromatographic column size is 250 × 20 mm, the stationary phase of the reversed-phase preparative column is a reversed-phase column (X10) with the diameter of 7 μm, and the mobile phase is a 25% methanol-water solution; hydrophilic preparative column the stationary phase was a 5 μm zwitterionic column Click XION and the mobile phase was a 94% acetonitrile-water solution.

Technical Field

The invention relates to the technical field of natural pharmaceutical chemistry, in particular to a separation and preparation method of a maltoside compound, namely, tunicoside B in dianthus superbus.

Background

Fringed pink (Chinese pink herb)Dianthus superbus L.) Genus Nerium (A. brevifiliformis)Gypsophila L.) And Caryophyllaceae (Caryophylllaceae), which are commonly used traditional Chinese medicines and contain various chemical components such as saponins, cyclic peptides, flavonoids, phenolic acids, anthraquinones, amides, coumarins, volatile oil and the like.Modern pharmacological studies show that the compound has multiple effects of resisting bacteria, protecting kidney, improving blood sugar and insulin levels, resisting early pregnancy, resisting tumors, suppressing immunity, protecting nerves and the like. In order to accelerate the germplasm resource evaluation of dianthus superbus and the research and development steps of related new drugs, the development of an efficient preparation method of a high-purity chemical reference substance is particularly important, especially for a large-scale preparation technology.

At present, tunicoside B is found for the first time in psammosilene plant of psammosilene genus of Caryophyllaceae in 2019 (Qi small slope, field is reluctant, Shenyunheng, etc.. 2 new maltoside compounds [ J ] in psammosilene root, Chinese herbal medicine, 2019, 50 (11): 2513-2517.), but because the research utilizes the traditional phytochemical means, the experimental operation is more complicated and the compounds are not easy to separate and can not be produced in batch through a plurality of steps of extraction, macroporous resin column elution, reverse phase silica gel column elution, silica gel column chromatography separation, reverse phase silica gel ODS (50 μm) medium pressure liquid phase column chromatography, Sephadex LH-20 column chromatography and the like; in addition, no literature report on the scale-up and rapid preparation of the compound tunicoside B from dianthus superbus is available. Therefore, a method which is simple in process and can rapidly prepare the compound tunicoside B from the dianthus superbus in a large scale is needed to be established.

Disclosure of Invention

In view of the above problems, the present invention aims to provide a method for separating and preparing a maltoside compound, tunicoside B, from fringed pink.

A method for separating and preparing a maltoside compound, namely, tunicoside B in dianthus superbus, is characterized by comprising the following steps: the process specifically comprises the following steps:

step 1, extraction: drying the fringed pink medicinal material in the shade, coarsely crushing the fringed pink medicinal material, and mixing the raw materials according to a material-liquid ratio of 1 g: extracting with 5-100 mL of ethanol at room temperature for 2-4 times, each time for 2-4 hours, filtering, and combining the filtrates to obtain a filtrate A, wherein the filtrate A is obtained by mixing the following components in parts by weight of silica gel: the weight of the dianthus superbus medicinal materials =1: 5-15 mixing the sample and drying under reduced pressure to obtain a mixed sample of the dianthus superbus extract;

step 2, carrying out medium-pressure chromatography rough separation on microporous resin: mixing the Dianthus superbus extract with sample, subjecting the sample to medium pressure chromatographic separation with microporous resin, detecting with ultraviolet detector with detection wavelength of 210 nm, collecting the fourth chromatographic peak fraction in the preparative chromatogram, and drying under reduced pressure to obtain target component Fr 4;

and 3, enriching by reverse phase medium pressure chromatography: mixing the target component Fr4 with silica gel to obtain a sample, separating the sample by a reverse phase medium pressure chromatographic column filled with silica gel matrix material, detecting by an ultraviolet detector with the detection wavelength of 210 nm, collecting the fraction of the fourth chromatographic peak in the preparative chromatogram, and drying the fraction under reduced pressure to obtain a component Fr4-4 containing the target component;

and 4, purifying by reversed phase/hydrophilic high-pressure two-dimensional liquid chromatography: dissolving a component Fr4-4 containing a target component in a methanol-water solution with the volume fraction of 50-100%, preparing a sample with the concentration of 20.0-50.0 mg/mL, filtering with a 0.45-micrometer microporous filter membrane to obtain a filtrate, namely a filtrate B, separating the filtrate B by reverse-phase high-pressure liquid chromatography, detecting by an ultraviolet detector with the detection wavelength of 210 nm, collecting a first chromatographic peak fraction in a reverse-phase high-pressure preparation chromatogram of the filtrate B, and drying under reduced pressure to obtain a component Fr441 containing the target component; dissolving a component Fr441 containing a target component by using a methanol solution with the volume fraction of 100%, preparing a sample with the concentration of 25.0 mg/mL, filtering the sample by using a 0.45-micrometer microporous filter membrane to obtain a filtrate C, purifying the filtrate C by using hydrophilic high-pressure liquid chromatography, detecting the purified filtrate C by using an ultraviolet detector with the detection wavelength of 210 nm, collecting a first chromatographic peak fraction Fr4411 in a hydrophilic high-pressure preparation chromatogram of the filtrate C, and drying the chromatographic peak fraction under reduced pressure to obtain a tunicoside B chemical reference substance with the purity of more than 98%.

Further, in step 1, step 2, step 3 and step 4, the conditions of reduced pressure drying are as follows: the vacuum degree is 50-250 mbar, and the temperature is 40-60 ℃;

further, in the step 2, the separation working parameters of the pressure chromatography rough separation in the microporous resin are as follows: the length of a chromatographic column is 460 mm, the diameter of the chromatographic column is 49 mm, the stationary phase of a microporous resin column is HP20SS, the mobile phase A is water, the mobile phase B is methanol, the mobile phase C is dichloromethane, the chromatographic conditions are 0-20 min, 0% B, 20-240 min, 0% -100% B, 240-300 min, 100% B, 300-420 min and 0-100% C, the sample injection amount is 10-80 g, and the flow rate is 40-60 mL/min;

further, in the step 3, the working parameters of the reverse phase medium pressure chromatographic enrichment are as follows: the length of a chromatographic column is 500 mm, the diameter of the chromatographic column is 50 mm, the stationary phase of a reversed-phase preparation column is Spherical C18 with the diameter of 50 microns, a mobile phase A is water, a mobile phase B is methanol, the chromatographic condition is 0-120 min, the concentration of B is 20-65%, the sample injection amount is 10-30 g, and the flow rate is 40-60 mL/min;

further, in the step 4, the working parameters of the reversed phase/hydrophilic high-pressure two-dimensional liquid chromatography purification are as follows: the chromatographic column size is 250 × 20 mm, the stationary phase of the reversed-phase preparative column is a reversed-phase column (X10) with the diameter of 7 μm, and the mobile phase is a 25% methanol-water solution; hydrophilic preparative column the stationary phase was a 5 μm zwitterionic column Click XION and the mobile phase was a 94% acetonitrile-water solution.

Compared with the prior art, the invention has the following advantages:

(1) the invention has low cost and high product purity

The used extraction solvent, the solvent used for the microporous resin medium pressure chromatography rough separation, the reversed phase medium pressure chromatography enrichment, the reversed phase and the hydrophilic high pressure chromatography separation can be recycled; the used chromatographic separation materials (microporous resin, reversed phase preparative liquid chromatography and hydrophilic preparative liquid chromatography separation materials) can be recycled; the recycled solvent and the recycled separation material ensure that the average cost in the separation process is low, and the purity of the product can be ensured to be more than 98% by two-step medium-pressure separation (microporous resin medium-pressure chromatography rough separation and reversed-phase medium-pressure chromatography enrichment) and high-pressure liquid chromatography purification.

(2) The preparation method can meet the requirement of large-scale production

The raw material requirement is not high, the cost is low, the Chinese pink herb is extremely easy to obtain, and the Chinese pink herb is only required to be planted generally or sold on the market and is easy to prepare in batches; the ethanol is extracted at room temperature, so the operation is easy; the separation adopts a microporous resin column for rough separation, and the microporous resin separation material can be arranged in a medium-pressure column chromatography system, so that the large-scale separation is easy to realize; the reversed phase/hydrophilic high pressure preparation liquid chromatogram used in the separation and purification is a quick isocratic method and is very suitable for large-scale production.

Drawings

FIG. 1 is a microporous resin separation chromatogram of a sample according to the present invention;

FIG. 2 is a reverse phase medium pressure chromatographic enrichment chromatogram of a microporous resin separation fraction Fr4 according to the invention;

FIG. 3 is a reversed-phase preparative liquid chromatography separation chart of fringed pink target component Fr4-4 according to the present invention;

FIG. 4 is a liquid chromatography separation chart of hydrophilic preparation of fringed pink target component Fr441 according to the present invention

FIG. 5 is a chromatogram for verifying purity of maltoside B in fringed pink;

FIG. 6 is a peak mass spectrum of an ESI-MS molecular ion of a tunicoside B chemical reference isolated according to the present invention;

FIG. 7 is a recorded chart of optical rotation measurement of a tunicoside B chemical reference substance separated according to the present invention

FIG. 8 is a UV spectrum of a tunicoside B chemical reference substance isolated according to the present invention

FIG. 9 is an isolated Tunicaside B chemical control fuchsin external spectrum of the present invention

FIG. 10 shows a tunicoside B chemical reference isolated according to the present invention¹H NMR nuclear magnetic map;

FIG. 11 shows a tunicoside B chemical reference isolated according to the present invention¹³C NMR nuclear magnetic map;

FIG. 12 is a schematic plan view of a tunicoside B chemical control isolated according to the present invention.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

A method for separating and preparing a maltoside compound, namely, tunicoside B in dianthus superbus comprises the following steps:

step 1, extraction: drying herba Dianthi in the shade, pulverizing, adding 10 times of ethanol, extracting at room temperature for 2 hr for 4 times, filtering, and mixing filtrates to obtain filtrate A, wherein the filtrate A is based on silica gel: mixing herba Dianthi medicinal material at a ratio of =1:15, and drying under reduced pressure to obtain 827 g of herba Dianthi ethanol extract sample mixture, wherein the reduced pressure drying condition is vacuum degree of 50 mbar and temperature of 40 deg.C;

step 2, carrying out medium-pressure chromatography rough separation on microporous resin: separating the Dianthus superbus ethanol extract sample by a medium-pressure chromatographic column filled with microporous resin materials, detecting by an ultraviolet detector with a detection wavelength of 210 nm, collecting a fourth main chromatographic peak fraction Fr4 (shown in figure 1) in a preparative chromatogram, and drying under reduced pressure to obtain a component Fr4 containing a target component: 159 g;

wherein the reduced pressure drying condition is vacuum degree of 50 mbar and temperature of 40 deg.C; the working parameters of the microporous resin medium pressure chromatography rough separation are as follows: the length of a chromatographic column is 460 mm, the diameter of the chromatographic column is 49 mm, the stationary phase of a microporous resin column is HP20SS, the mobile phase A is water, the mobile phase B is methanol, the mobile phase C is dichloromethane, the chromatographic conditions are 0-20 min, 0% B, 20-240 min, 0% -100% B, 240-300 min, 100% B, 300-420 min and 0-100% C, the sample injection amount is 80 g, and the flow rate is 60 mL/min;

and 3, enriching by reverse phase medium pressure chromatography: mixing the target component Fr4 with silica gel to obtain a sample, separating the sample by a reverse phase medium pressure chromatographic column filled with silica gel matrix material, detecting by an ultraviolet detector with a detection wavelength of 210 nm, collecting a fourth main chromatographic peak fraction Fr4-4 (shown in figure 2) in a preparative chromatogram, and drying the fraction under reduced pressure to obtain a component Fr4-4 containing a target component: 5.7 g;

wherein the reduced pressure drying condition is vacuum degree of 50 mbar and temperature of 40 deg.C; the separation working parameters of the reversed-phase medium-pressure chromatographic enrichment are as follows: the length of a chromatographic column is 500 mm, the diameter of the chromatographic column is 50 mm, the stationary phase of a reversed-phase preparation column is Spherical C18 with the diameter of 50 microns, a mobile phase A is water, a mobile phase B is methanol, the chromatographic condition is 0-120 min, the concentration of B is 20% -65%, the sample injection amount is 30 g, and the flow rate is 60 mL/min;

and 4, purifying by reversed phase/hydrophilic two-dimensional liquid chromatography: dissolving component Fr4-4 containing target component with 50% methanol-water solution by volume fraction, preparing sample with concentration of 50.0 mg/mL, filtering with 0.45 μm microporous membrane to obtain filtrate B, separating by reversed phase high pressure liquid chromatography, detecting with ultraviolet detector with detection wavelength of 210 nm, collecting the first main chromatographic peak fraction in the reversed phase high pressure preparative chromatogram of filtrate B, and drying under reduced pressure to obtain component Fr441 (shown in figure 3) containing target component; dissolving a component Fr441 containing a target component in a methanol solution with the volume fraction of 100%, preparing a sample with the concentration of 25.0 mg/mL, filtering the sample through a 0.45-micrometer microporous filter membrane to obtain a filtrate C, purifying the filtrate C through hydrophilic high-pressure liquid chromatography, detecting the purified filtrate C through an ultraviolet detector with the detection wavelength of 210 nm, collecting a first main chromatographic peak fraction Fr4411 (shown in figure 4) in a hydrophilic high-pressure preparation chromatogram of the filtrate C, and drying the chromatographic peak fraction under reduced pressure to obtain a tunaoside B chemical reference substance with the purity of more than 98 percent, wherein the first main chromatographic peak fraction Fr4411 is shown in figure 4;

wherein, the working parameters of the reversed phase/hydrophilic high-pressure two-dimensional liquid chromatography purification are as follows: the sizes of chromatographic columns are 250 multiplied by 20 mm, the stationary phase of the reversed phase preparative column is a reversed phase column X10 with the diameter of 7 mu m, the mobile phase is a 25% methanol-water solution, the sample injection volume is 4 mL, and the flow rate is 19 mL/min; the fixed phase of the hydrophilic preparation column is a 5 mu m zwitterionic column Click XION, the mobile phase is a 94% acetonitrile-water solution, the sample injection volume is 4 mL, and the flow rate is 19 mL/min.

Example 2

A method for separating and preparing a maltoside compound, namely, tunicoside B in dianthus superbus comprises the following steps:

step 1, extraction: taking 10 kg of fringed pink dried in the shade, crushing, adding ethanol with the mass 10 times of that of the fringed pink dried in the shade, extracting for 3 times at room temperature for 3 hours each time, filtering, and combining filtrate to obtain filtrate A, wherein the filtrate A is obtained according to the weight of silica gel: mixing herba Dianthi medicinal material at a ratio of =1:8, and drying under reduced pressure to obtain 2.66 kg of mixed sample of herba Dianthi ethanol extract, wherein the reduced pressure drying condition is vacuum degree of 250 mbar and temperature of 60 deg.C;

step 2, carrying out medium-pressure chromatography rough separation on microporous resin: separating the sample by a medium-pressure chromatographic column filled with microporous resin materials, detecting by an ultraviolet detector with the detection wavelength of 210 nm, collecting a fourth main chromatographic peak fraction Fr4 in a preparative chromatogram, and drying under reduced pressure to obtain a component Fr 4: 392 g;

wherein the reduced pressure drying condition is vacuum degree of 250 mbar and temperature of 60 deg.C; the working parameters of the microporous resin medium pressure chromatography rough separation are as follows: the length of a chromatographic column is 460 mm, the diameter of the chromatographic column is 49 mm, the stationary phase of a microporous resin column is HP20SS, the mobile phase A is water, the mobile phase B is methanol, the mobile phase C is dichloromethane, the chromatographic conditions are 0-20 min, 0% B, 20-240 min, 0% -100% B, 240-300 min, 100% B, 300-420 min and 0-100% C, the sample injection amount is 40 g, and the flow rate is 50 mL/min;

and 3, enriching by reverse phase medium pressure chromatography: and mixing the target component Fr4 with silica gel to obtain a sample, separating the sample by a reverse phase medium pressure chromatographic column filled with a silica gel matrix material, detecting by an ultraviolet detector with the detection wavelength of 210 nm, collecting a fourth main chromatographic peak fraction Fr4-4 in a preparative chromatogram, and drying the fraction under reduced pressure to obtain a component Fr4-4 containing a target component: 13.8 g;

wherein the reduced pressure drying condition is vacuum degree of 250 mbar and temperature of 60 deg.C; the separation working parameters of the reversed-phase medium-pressure chromatographic enrichment are as follows: the length of a chromatographic column is 500 mm, the diameter of the chromatographic column is 50 mm, the stationary phase of a reversed-phase preparation column is Spherical C18 with the diameter of 50 microns, a mobile phase A is water, a mobile phase B is methanol, the chromatographic condition is 0-120 min, the concentration of B is 20-65%, the sample injection amount is 15 g, and the flow rate is 45 mL/min;

and 4, purifying by reversed phase/hydrophilic two-dimensional liquid chromatography: dissolving a component Fr4-4 containing a target component in 80% methanol-water solution by volume fraction, preparing a sample with the concentration of 40.0 mg/mL, filtering with a 0.45-micrometer microporous membrane to obtain a filtrate B, separating the filtrate B by reverse-phase high-pressure liquid chromatography, detecting with an ultraviolet detector with the detection wavelength of 210 nm, collecting the first main chromatographic peak fraction in the reverse-phase high-pressure preparative chromatogram of the filtrate B, and drying under reduced pressure to obtain a component Fr441 containing the target component; dissolving a component Fr441 containing a target component by using a methanol solution with the volume fraction of 100%, preparing a sample with the concentration of 25.0 mg/mL, filtering the sample by using a 0.45-micrometer microporous filter membrane to obtain a filtrate C, purifying the filtrate C by using hydrophilic high-pressure liquid chromatography, detecting the purified filtrate C by using an ultraviolet detector with the detection wavelength of 210 nm, collecting a first main chromatographic peak fraction Fr4411 in a hydrophilic high-pressure preparation chromatogram of the filtrate C, and drying the chromatographic peak fraction under reduced pressure to obtain 135 mg of a tunicoside B chemical reference substance with the purity of more than 98%;

wherein, the working parameters of the reversed phase/hydrophilic high-pressure two-dimensional liquid chromatography purification are as follows: the sizes of chromatographic columns are 250 multiplied by 20 mm, the stationary phase of the reversed phase preparative column is a reversed phase column X10 with the diameter of 7 mu m, the mobile phase is a 25% methanol-water solution, the sample injection volume is 4 mL, and the flow rate is 19 mL/min; the fixed phase of the hydrophilic preparation column is a 5 mu m zwitterionic column Click XION, the mobile phase is a 94% acetonitrile-water solution, the sample injection volume is 4 mL, and the flow rate is 19 mL/min.

Example 3

A method for separating and preparing a maltoside compound tunicoside B chemical reference substance in dianthus superbus comprises the following steps:

step 1, extraction: 1 kg of fringed pink dried in the shade is taken, crushed and added with ethanol with the weight 10 times of that of the fringed pink dried in the shade, the fringed pink dried in the shade is extracted for 2 times at room temperature for 4 hours each time, and the filtrate A is filtered and combined, namely the filtrate A, and the filtrate A is obtained according to the weight of silica gel: mixing herba Dianthi medicinal material at a ratio of 1:5, and drying under reduced pressure to obtain 338 g of herba Dianthi ethanol extract sample mixture, wherein the reduced pressure drying condition is vacuum degree of 150 mbar and temperature of 50 deg.C;

step 2, carrying out medium-pressure chromatography rough separation on microporous resin: separating the sample by a medium-pressure chromatographic column filled with microporous resin materials, detecting by an ultraviolet detector with the detection wavelength of 210 nm, collecting a fourth main chromatographic peak fraction Fr4 in a preparative chromatogram, and drying under reduced pressure to obtain a component Fr 4: 38.7 g;

wherein the reduced pressure drying condition is vacuum degree of 150 mbar and temperature of 50 deg.C; the working parameters of the microporous resin medium pressure chromatography rough separation are as follows: the length of a chromatographic column is 460 mm, the diameter of the chromatographic column is 49 mm, the stationary phase of a microporous resin column is HP20SS, the mobile phase A is water, the mobile phase B is methanol, the mobile phase C is dichloromethane, the chromatographic conditions are 0-20 min, 0% B, 20-240 min, 0% -100% B, 240-300 min, 100% B, 300-420 min and 0-100% C, the sample injection amount is 10 g, and the flow rate is 40 mL/min;

and 3, enriching by reverse phase medium pressure chromatography: and mixing the target component Fr4 with silica gel to obtain a sample, separating the sample by a reverse phase medium pressure chromatographic column filled with a silica gel matrix material, detecting by an ultraviolet detector with the detection wavelength of 210 nm, collecting a fourth main chromatographic peak fraction Fr4-4 in a preparative chromatogram, and drying the fraction under reduced pressure to obtain a component Fr4-4 containing a target component: 1.4 g.

Wherein the reduced pressure drying condition is vacuum degree of 150 mbar and temperature of 50 deg.C; the separation working parameters of the reversed-phase medium-pressure chromatographic enrichment are as follows: the length of a chromatographic column is 500 mm, the diameter of the chromatographic column is 50 mm, the stationary phase of a reversed-phase preparation column is Spherical C18 with the diameter of 50 microns, a mobile phase A is water, a mobile phase B is methanol, the chromatographic condition is 0-120 min, the concentration of B is 20% -65%, the sample injection amount is 10 g, and the flow rate is 40 mL/min.

And 4, purifying by reversed phase/hydrophilic two-dimensional liquid chromatography: dissolving a component Fr4-4 containing a target component in a methanol-water solution with the volume fraction of 100%, preparing a sample with the concentration of 20.0 mg/mL, filtering with a 0.45-micrometer microporous membrane to obtain a filtrate B, separating the filtrate B by reverse-phase high-pressure liquid chromatography, detecting with an ultraviolet detector with the detection wavelength of 210 nm, collecting the first main chromatographic peak fraction in the reverse-phase high-pressure preparative chromatogram of the filtrate B, and drying under reduced pressure to obtain a component Fr441 containing the target component; dissolving a component Fr441 containing a target component by using a methanol solution with the volume fraction of 100%, preparing a sample with the concentration of 25.0 mg/mL, filtering the sample by using a 0.45-micrometer microporous filter membrane to obtain a filtrate C, purifying the filtrate C by using hydrophilic high-pressure liquid chromatography, detecting the purified filtrate C by using an ultraviolet detector with the detection wavelength of 210 nm, collecting a first main chromatographic peak fraction Fr4411 in a hydrophilic high-pressure preparation chromatogram of the filtrate C, and drying the chromatographic peak fraction under reduced pressure to obtain 14 mg of a tunicoside B chemical reference substance with the purity of more than 98%.

Wherein, the working parameters of the reversed phase/hydrophilic high-pressure two-dimensional liquid chromatography purification are as follows: the sizes of chromatographic columns are 250 multiplied by 20 mm, the stationary phase of the reversed phase preparative column is a reversed phase column X10 with the diameter of 7 mu m, the mobile phase is a 25% methanol-water solution, the sample injection volume is 4 mL, and the flow rate is 19 mL/min; the fixed phase of the hydrophilic preparation column is a 5 mu m zwitterionic column Click XION, the mobile phase is a 94% acetonitrile-water solution, the sample injection volume is 4 mL, and the flow rate is 19 mL/min.

Wherein the obtained Fr4411 is a tunicoside B sample with the purity of more than 98 percent, and a purity verification chromatogram (shown in figure 5);

the structural representation and structure of the maltoside compound tunicoside B obtained from the dianthus superbus are shown in the attached figures 6-12, which proves that the maltoside compound tunicoside B is successfully separated by the method.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.