阅读说明：本技术 一种通过胚拯救获得重瓣萱草杂交后代的方法 (Method for obtaining filial generation of hemerocallis sempervirens through embryo rescue ) 是由 陈芬 马丽 刘博� 蔡卫佳 王昊 于 2021-10-18 设计创作，主要内容包括：本发明提供一种通过受精幼胚获得重重瓣萱草杂种后代的方法属于植物组织培养技术的植物再生技术领域,其方法是通过取人工去雄授粉9d的胚珠采用离体培养的方式进行胚拯救,具体步骤为：(1)授粉,(2)取胚珠,(3)诱导培养,(4)继代培养,(5)生根培养,(6)移栽；本发明弥补了重瓣萱草杂交结实率低下的问题,避免了外界环境的不确定性对成活率的影响,同时也缩短了育种周期,可运用于重瓣萱草杂交育种工作。(The invention provides a method for obtaining a hybrid progeny of hemerocallis fulva by fertilizing a young embryo, which belongs to the technical field of plant regeneration of plant tissue culture technology, and the method comprises the following steps of carrying out embryo rescue by taking ovules which are artificially emasculated and pollinated for 9d in an in vitro culture mode: (1) pollinating, (2) taking ovules, (3) performing induction culture, (4) performing subculture, (5) performing rooting culture, and (6) transplanting; the method solves the problem of low fruiting rate of Hemerocallis fulva hybrid, avoids the influence of uncertainty of external environment on survival rate, shortens breeding period, and can be applied to Hemerocallis fulva hybrid breeding work.)