阅读说明：本技术 一种纳米脂质体及其制备方法与应用以及一种抗炎修复霜、抗炎修复乳和抗炎修复喷雾 (Nano-liposome, preparation method and application thereof, and anti-inflammatory repair cream, anti-inflammatory repair emulsion and anti-inflammatory repair spray ) 是由 刘帅 李阳 周建青 刘敏 徐晓平 于 2021-10-13 设计创作，主要内容包括：本发明涉及一种纳米脂质体及其制备方法与应用以及一种抗炎修复霜、抗炎修复乳和抗炎修复喷雾,涉及化妆品技术领域。本发明的纳米脂质体包括如下质量份数的组分：分子量为150万道尔顿的透明质酸钠0.15～0.3份、分子量为40万道尔顿的透明质酸钠0.25～0.6份、分子量为0.5万道尔顿的透明质酸钠0.02～0.05份、甘油1～25份、1,2-戊二醇2～5份、季铵盐-730.001～0.005份、D-泛醇0.4～2份、β-葡聚糖0.001～0.2份、烟酰胺1～2.5份、氢化卵磷脂1～5份、水59.345～94.178份。本发明的纳米脂质体配方合理,具有长效的抗炎功效,能从根本上修复皮肤屏障。(The invention relates to a nano liposome, a preparation method and application thereof, and an anti-inflammatory repair cream, an anti-inflammatory repair emulsion and an anti-inflammatory repair spray, and relates to the technical field of cosmetics. The nanoliposome comprises the following components in parts by weight: 0.15-0.3 part of sodium hyaluronate with the molecular weight of 150 kilodalton, 0.25-0.6 part of sodium hyaluronate with the molecular weight of 40 kilodalton, 0.02-0.05 part of sodium hyaluronate with the molecular weight of 0.5 kilodalton, 1-25 parts of glycerol, 2-5 parts of 1, 2-pentanediol, 0.005-0. 730.001 part of quaternary ammonium salt, 0.4-2 parts of D-panthenol, 0.001-0.2 part of beta-glucan, 1-2.5 parts of nicotinamide, 1-5 parts of hydrogenated lecithin and 59.345-94.178 parts of water. The nano liposome has reasonable formula, long-acting anti-inflammatory effect and can fundamentally repair skin barriers.)

1. The nanoliposome is characterized by comprising the following components in parts by mass: 0.15-0.3 part of sodium hyaluronate with the molecular weight of 150 kilodalton, 0.25-0.6 part of sodium hyaluronate with the molecular weight of 40 kilodalton, 0.02-0.05 part of sodium hyaluronate with the molecular weight of 0.5 kilodalton, 1-25 parts of glycerol, 2-5 parts of 1, 2-pentanediol, 0.005-0. 730.001 part of quaternary ammonium salt, 0.4-2 parts of D-panthenol, 0.001-0.2 part of beta-glucan, 1-2.5 parts of nicotinamide, 1-5 parts of hydrogenated lecithin and 59.345-94.178 parts of water.

2. The nanoliposome according to claim 1, which comprises the following components by mass: 0.2-0.25 part of sodium hyaluronate with the molecular weight of 150 kilodalton, 0.35-0.5 part of sodium hyaluronate with the molecular weight of 40 kilodalton, 0.03-0.04 part of sodium hyaluronate with the molecular weight of 0.5 kilodalton, 9-17 parts of glycerol, 3-4 parts of 1, 2-pentanediol, 0.004 part of quaternary ammonium salt-730.002-0.004 part of D-panthenol, 0.8-1.6 parts of beta-glucan, 0.091-0.11 part of nicotinamide, 1.5-2 parts of hydrogenated lecithin and 69.345-84.178 parts of water.

3. The nanoliposome according to claim 1 or 2, which comprises the following components by mass: 0.225 part of sodium hyaluronate with the molecular weight of 150 ten thousand daltons, 0.4 part of sodium hyaluronate with the molecular weight of 40 ten thousand daltons, 0.035 part of sodium hyaluronate with the molecular weight of 0.5 ten thousand daltons, 13 parts of glycerol, 3.5 parts of 1, 2-pentanediol, 730.003 parts of quaternary ammonium salt-730.003 parts of D-panthenol 1.2 parts, 0.1005 part of beta-glucan, 1.75 parts of nicotinamide, 3 parts of hydrogenated lecithin and 76.7615 parts of water.

4. The method for preparing nanoliposomes according to any one of claims 1 to 3, comprising the steps of:

(1) mixing sodium hyaluronate with molecular weight of 150 kilodalton, sodium hyaluronate with molecular weight of 40 kilodalton, sodium hyaluronate with molecular weight of 0.5 kilodalton, glycerol and water to obtain phase A;

(2) mixing 1, 2-pentanediol, quaternary ammonium salt-73, D-panthenol, beta-glucan, nicotinamide and hydrogenated lecithin with the A phase, and homogenizing to obtain the nano liposome.

5. The method according to claim 4, wherein the mixing in step (1) is performed in a homogeneous manner;

the homogenizing temperature is 80-85 ℃;

the homogenizing time is 2-4 min;

the rotation speed of the homogenization is 2000-3000 r/min;

the vacuum degree during homogenizing is-0.03 to-0.07 MPa.

6. The method according to claim 4 or 5, wherein the temperature of phase A is 60 to 70 ℃ when the 1, 2-pentanediol, the quaternary ammonium salt-73, the D-panthenol, the beta-glucan, the nicotinamide and the hydrogenated lecithin are mixed with phase A;

the homogenization in the step (2) comprises a first homogenization and a second homogenization which are carried out in sequence;

the rotating speed of the first homogenizing is 6000-30000 r/min;

the first homogenization time is 5-30 min;

the vacuum degree of the first homogeneity is-0.03 to-0.07 MPa;

the pressure of the second homogenizing is 60-150 MPa;

the number of times of the second homogenization is 1-6;

the time for the second homogenization is 30-45 s each time.

7. Use of nanoliposomes according to any one of claims 1 to 3 in the preparation of a cosmetic with anti-inflammatory repair effect;

the cosmetic is anti-inflammatory repair cream, anti-inflammatory repair emulsion or anti-inflammatory repair spray.

8. The anti-inflammatory repair cream is characterized by comprising the following components in percentage by mass:

water phase: oil phase: carbomer: xanthan gum: arginine: deionized water: the nanoliposome is 79-81: 12-13: 0.25-0.35: 0.1-0.2: 0.25-0.35: 4.5-5.5: 1.5-2.5;

the water phase comprises the following components in parts by weight: 69-71 parts of deionized water, 3.5-4.5 parts of glycerol, 4-5 parts of 1, 3-butanediol, 0.04-0.06 part of EDTA-2Na, 0.4-0.6 part of 1, 2-hexanediol and 0.4-0.6 part of p-hydroxyacetophenone;

the oil phase comprises the following components in parts by mass: 2-4 parts of caprylic/capric triglyceride, 2-3 parts of squalane, 1-2 parts of cetostearyl alcohol, 0.5-1.5 parts of shea butter, 1-3 parts of chinaroot greenbrier seed oil and 2-3 parts of glyceryl stearate citrate.

9. The anti-inflammatory repair emulsion is characterized by comprising the following components in percentage by mass:

water phase: oil phase: carbomer: xanthan gum: arginine: deionized water: the nano liposome is 82-84: 8-10: 0.1-0.2: 0.1-0.14: 0.1-0.2: 4-6: 1.5-2.5;

the water phase comprises the following components in parts by weight: 74-76 parts of deionized water, 3-5 parts of glycerol, 2-4 parts of 1, 3-butanediol, 0.4-0.6 part of 1, 2-hexanediol and 0.4-0.6 part of p-hydroxyacetophenone;

the oil phase comprises the following components in parts by mass: 2-4 parts of caprylic/capric triglyceride, 0.5-1.5 parts of squalane, 0.4-0.6 part of cetostearyl alcohol, 0.5-1.5 parts of shea butter, 1-2 parts of chinlon oil and 1.5-2.5 parts of glyceryl stearate citrate.

10. An anti-inflammatory repair spray is characterized by comprising the following components in percentage by mass:

water phase: carbomer: xanthan gum: arginine: deionized water: the nanoliposome is 92-93: 0.05-0.15: 4-6: 1.5-2.5;

the water phase comprises the following components in parts by weight: 85-86 g of deionized water, 3-5 g of glycerol, 2-4 g of 1, 3-butanediol, 0.4-0.6 g of 1, 2-hexanediol and 0.4-0.6 g of p-hydroxyacetophenone.

Technical Field

The invention relates to the technical field of cosmetics, in particular to a nano liposome, a preparation method and application thereof, and an anti-inflammatory repair cream, an anti-inflammatory repair emulsion and an anti-inflammatory repair spray.

Background

Inflammation (inflammation) is what is commonly called "inflammation" and is a defense response of the body to stimuli, manifested by redness, swelling, heat, pain, and dysfunction. The inflammation may be infectious inflammation caused by infection or non-infectious inflammation not caused by infection. Often, inflammation is beneficial and is an automatic defense response of the human body; sometimes, however, inflammation is also harmful, such as attack on the body's own tissues, inflammation occurring in transparent tissues, and the like. In the course of inflammation, on the one hand, the damage factors cause directly or indirectly the destruction of tissues and cells; on the other hand, the inflammatory hyperemia and exudation reaction dilutes, kills and surrounds the injury factor, and simultaneously, the injured tissues are repaired and healed through regeneration of parenchymal and interstitial cells. Inflammation can therefore be said to be a uniform process of injury and anti-injury. Inflammation is most marked by the manifestation of inflammation of the body surface, and the influence of inflammation on the skin barrier is shown as follows: the epidermis water loss rate is increased, and the skin water retention is reduced; ② the skin is dry, tight and even desquamation; and thirdly, the keratin formation, the cell growth and the recovery of the damaged skin are difficult. Mild skin inflammation occurs such as: sensitive skin, and damaged skin barrier. Severe cases may suffer from different degrees of skin disorders such as: acne, acne rosacea, seborrheic dermatitis, hormone-dependent dermatitis, perioral dermatitis, chloasma, etc.

At present, clinically, the treatment for skin inflammation mainly takes medicines as main materials, and special auxiliary skin care products are lacked. Even though some anti-inflammatory skin care products exist in the prior art, the anti-inflammatory effect is not ideal due to unreasonable formula. Therefore, a skin care product with reasonable formula and good anti-inflammatory effect is urgently needed to meet the market demand.

Disclosure of Invention

The invention aims to provide a nano liposome with obvious anti-inflammatory repair effect, a preparation method and application thereof, and anti-inflammatory repair cream, anti-inflammatory repair emulsion and anti-inflammatory repair spray.

In order to achieve the above object, the present invention provides the following technical solutions:

a nanoliposome comprises the following components in parts by weight: 0.15-0.3 part of sodium hyaluronate with the molecular weight of 150 kilodalton, 0.25-0.6 part of sodium hyaluronate with the molecular weight of 40 kilodalton, 0.02-0.05 part of sodium hyaluronate with the molecular weight of 0.5 kilodalton, 1-25 parts of glycerol, 2-5 parts of 1, 2-pentanediol, 0.005-0. 730.001 part of quaternary ammonium salt, 0.4-2 parts of D-panthenol, 0.001-0.2 part of beta-glucan, 1-2.5 parts of nicotinamide, 1-5 parts of hydrogenated lecithin and 59.345-94.178 parts of water.

Preferably, the composition comprises the following components in parts by mass: 0.2-0.25 part of sodium hyaluronate with the molecular weight of 150 kilodalton, 0.35-0.5 part of sodium hyaluronate with the molecular weight of 40 kilodalton, 0.03-0.04 part of sodium hyaluronate with the molecular weight of 0.5 kilodalton, 9-17 parts of glycerol, 3-4 parts of 1, 2-pentanediol, 0.004 part of quaternary ammonium salt-730.002-0.004 part of D-panthenol, 0.8-1.6 parts of beta-glucan, 0.091-0.11 part of nicotinamide, 1.5-2 parts of hydrogenated lecithin and 69.345-84.178 parts of water.

Preferably, the composition comprises the following components in parts by mass: 0.225 part of sodium hyaluronate with the molecular weight of 150 ten thousand daltons, 0.4 part of sodium hyaluronate with the molecular weight of 40 ten thousand daltons, 0.035 part of sodium hyaluronate with the molecular weight of 0.5 ten thousand daltons, 13 parts of glycerol, 3.5 parts of 1, 2-pentanediol, 730.003 parts of quaternary ammonium salt-730.003 parts of D-panthenol 1.2 parts, 0.1005 part of beta-glucan, 1.75 parts of nicotinamide, 3 parts of hydrogenated lecithin and 76.7615 parts of water.

The invention also provides a preparation method of the nanoliposome, which comprises the following steps:

(1) mixing sodium hyaluronate with molecular weight of 150 kilodalton, sodium hyaluronate with molecular weight of 40 kilodalton, sodium hyaluronate with molecular weight of 0.5 kilodalton, glycerol and water to obtain phase A;

(2) mixing 1, 2-pentanediol, quaternary ammonium salt-73, D-panthenol, beta-glucan, nicotinamide and hydrogenated lecithin with the A phase, and homogenizing to obtain the nano liposome.

Preferably, the mixing in step (1) is homogeneous;

the homogenizing temperature is 80-85 ℃;

the homogenizing time is 2-4 min;

the rotation speed of the homogenization is 2000-3000 r/min;

the vacuum degree during homogenizing is-0.03 to-0.07 MPa.

Preferably, when the 1, 2-pentanediol, the quaternary ammonium salt-73, the D-panthenol, the beta-glucan, the nicotinamide and the hydrogenated lecithin are mixed with the phase A, the temperature of the phase A is 60-70 ℃;

the homogenization in the step (2) comprises a first homogenization and a second homogenization which are carried out in sequence;

the rotating speed of the first homogenizing is 6000-30000 r/min;

the first homogenization time is 5-30 min;

the vacuum degree of the first homogeneity is-0.03 to-0.07 MPa;

the pressure of the second homogenizing is 60-150 MPa;

the number of times of the second homogenization is 1-6;

the time of each second homogenization is 30-45 s

The invention also provides the application of the nano liposome in the preparation of cosmetics with anti-inflammatory and repair effects;

the cosmetic is anti-inflammatory repair cream, anti-inflammatory repair emulsion or anti-inflammatory repair spray.

An anti-inflammatory repair cream comprises the following components in percentage by mass:

water phase: oil phase: carbomer: xanthan gum: arginine: deionized water: the nanoliposome is 79-81: 12-13: 0.25-0.35: 0.1-0.2: 0.25-0.35: 4.5-5.5: 1.5-2.5;

the water phase comprises the following components in parts by weight: 69-71 parts of deionized water, 3.5-4.5 parts of glycerol, 4-5 parts of 1, 3-butanediol, 0.04-0.06 part of EDTA-2Na, 0.4-0.6 part of 1, 2-hexanediol and 0.4-0.6 part of p-hydroxyacetophenone;

the oil phase comprises the following components in parts by mass: 2-4 parts of caprylic/capric triglyceride, 2-3 parts of squalane, 1-2 parts of cetostearyl alcohol, 0.5-1.5 parts of shea butter, 1-3 parts of chinaroot greenbrier seed oil and 2-3 parts of glyceryl stearate citrate.

An anti-inflammatory repair emulsion comprises the following components in percentage by mass:

water phase: oil phase: carbomer: xanthan gum: arginine: deionized water: the nano liposome is 82-84: 8-10: 0.1-0.2: 0.1-0.14: 0.1-0.2: 4-6: 1.5-2.5;

the water phase comprises the following components in parts by weight: 74-76 parts of deionized water, 3-5 parts of glycerol, 2-4 parts of 1, 3-butanediol, 0.4-0.6 part of 1, 2-hexanediol and 0.4-0.6 part of p-hydroxyacetophenone;

the oil phase comprises the following components in parts by mass: 2-4 parts of caprylic/capric triglyceride, 0.5-1.5 parts of squalane, 0.4-0.6 part of cetostearyl alcohol, 0.5-1.5 parts of shea butter, 1-2 parts of chinlon oil and 1.5-2.5 parts of glyceryl stearate citrate.

An anti-inflammatory repair spray comprises the following components in percentage by mass:

water phase: carbomer: xanthan gum: arginine: deionized water: the nanoliposome is 92-93: 0.05-0.15: 4-6: 1.5-2.5;

the water phase comprises the following components in parts by weight: 85-86 g of deionized water, 3-5 g of glycerol, 2-4 g of 1, 3-butanediol, 0.4-0.6 g of 1, 2-hexanediol and 0.4-0.6 g of p-hydroxyacetophenone.

The invention provides a nano liposome, a preparation method and application thereof, and an anti-inflammatory repair cream, an anti-inflammatory repair emulsion and an anti-inflammatory repair spray, and compared with the prior art, the nano liposome has the following advantages:

(1) the invention preferably selects three different typesThe sodium hyaluronate with large molecular weight is organically combined, and the synergistic effect of hyaluronic acid with different molecular weights on inhibiting inflammatory reaction and promoting repair is exerted. And because the transdermal properties of the nicotinamide and the D-panthenol are good, part of the cationic active substances, namely the quaternary ammonium salt-73, the D-panthenol and the nicotinamide, act on the epidermal layer and the dermal layer together to achieve better sterilization and bacteriostasis effects. Conversion of nicotinamide to NDA in skin cells⁺And NDAP⁺And (3) the enzyme is supplemented, so that a DNA repair mechanism is improved, structural protein, lipid and collagen of a dermis layer of an epidermis layer are stimulated, and the cell structure is gradually restored to be complete.

(2) The nano liposome prepared by the preparation method can improve the transdermal absorption level and the sustained release capability and can better exert the effect.

(3) The nano-liposome has obvious inhibition effect on inflammatory factors, has long-acting anti-inflammatory effect and can fundamentally repair skin barriers.

Detailed Description

The invention provides a nanoliposome which comprises the following components in parts by weight: 0.15-0.3 part of sodium hyaluronate with the molecular weight of 150 ten thousand daltons, preferably 0.2-0.25 part, and further preferably 0.225 part;

0.25-0.6 part of sodium hyaluronate with the molecular weight of 40 ten thousand daltons, preferably 0.35-0.5 part, and further preferably 0.4 part;

0.02-0.05 part of sodium hyaluronate with the molecular weight of 0.5 ten thousand daltons, preferably 0.03-0.04 part, and further preferably 0.035 part;

1-25 parts of glycerol, preferably 9-17 parts of glycerol, and more preferably 13 parts of glycerol;

2-5 parts of 1, 2-pentanediol, preferably 3-4 parts, and more preferably 3.5 parts;

0.005 part of quaternary ammonium salt-730.001, preferably 0.002-0.004 part, and more preferably 0.003 part;

0.4-2 parts of D-panthenol, preferably 0.8-1.6 parts, and more preferably 1.2 parts;

0.001-0.2 part of beta-glucan, preferably 0.091-0.11 part of beta-glucan, and more preferably 0.1005 part of beta-glucan;

1-2.5 parts of nicotinamide, preferably 1.5-2 parts, and more preferably 1.75 parts;

1-5 parts of hydrogenated lecithin, preferably 2-4 parts, and more preferably 3 parts;

59.345-94.178 parts of water, preferably 69.345-84.178 parts of water, and more preferably 76.7615 parts of water.

The invention also provides a preparation method of the nanoliposome, which comprises the following steps:

(1) mixing sodium hyaluronate with molecular weight of 150 kilodalton, sodium hyaluronate with molecular weight of 40 kilodalton, sodium hyaluronate with molecular weight of 0.5 kilodalton, glycerol and water to obtain phase A;

(2) mixing 1, 2-pentanediol, quaternary ammonium salt-73, D-panthenol, beta-glucan, nicotinamide and hydrogenated lecithin with the A phase, and homogenizing to obtain the nano liposome.

In the invention, the mixing mode of the step (1) is homogenization;

the homogenizing temperature is 80-85 ℃, the preferable temperature is 81-84 ℃, and the further preferable temperature is 82.5 ℃;

the homogenizing time is 2-4 min, preferably 3 min;

the rotation speed of the homogenization is 2000-3000r/min, preferably 2500 r/min;

the degree of vacuum at the time of homogenization is-0.03 to-0.07 MPa, preferably-0.04 to-0.06 MPa, and more preferably-0.05 MPa.

In the present invention, when the 1, 2-pentanediol, the quaternary ammonium salt-73, the D-panthenol, the β -glucan, the nicotinamide, and the hydrogenated lecithin are mixed with the phase a, the temperature of the phase a is 60 to 70 ℃, preferably 62 to 68 ℃, and more preferably 65 ℃.

In the invention, the homogenization in the step (2) comprises a first homogenization and a second homogenization which are carried out in sequence.

The rotating speed of the first homogenizing device is 6000-30000 r/min, preferably 12000-24000 r/min, and more preferably 18000 r/min;

the first homogenizing time is 5-30 min, preferably 15-20 min, and further preferably 17.5 min;

the vacuum degree of the first homogenizing is-0.03 to-0.07 MPa, preferably-0.04 to-0.06 MPa, and further preferably-0.05 MPa;

the pressure of the second homogenizing is 60-150 MPa, preferably 80-130 MPa, and more preferably 105 MPa;

the number of times of the second homogenization is 1-6, preferably 2-5, and more preferably 3.5;

the time for each second homogenization is 30-45 s, preferably 25-40 s.

In the invention, the liquid obtained after the first homogenization is coarse milk dispersion.

In the invention, the liquid obtained by the second homogenization is clear and transparent.

The invention also provides application of the nano liposome in preparation of cosmetics with anti-inflammatory and repair effects.

The cosmetic is anti-inflammatory repair cream, anti-inflammatory repair emulsion or anti-inflammatory repair spray.

The invention also provides an anti-inflammatory repair cream which comprises the following components in percentage by mass:

water phase: oil phase: carbomer: xanthan gum: arginine: deionized water: the nanoliposome is 79-81: 12-13: 0.25-0.35: 0.1-0.2: 0.25-0.35: 4.5-5.5: 1.5-2.5; preferably the aqueous phase: oil phase: carbomer: xanthan gum: arginine: deionized water: the nano-liposome is 80:12.5:0.3:0.15:0.3:5: 2;

the water phase comprises the following components in parts by weight: 69-71 parts of deionized water, preferably 70 parts;

3.5-4.5 parts of glycerol, preferably 4 parts;

4-5 parts of 1, 3-butanediol, preferably 4.5 parts;

0.04-0.06 part of EDTA-2Na, preferably 0.05 part;

0.4-0.6 part of 1, 2-hexanediol, preferably 0.5 part;

0.4-0.6 part of p-hydroxyacetophenone, preferably 0.5 part.

The oil phase comprises the following components in parts by mass: 2-4 parts of caprylic/capric triglyceride, preferably 3 parts;

2-3 parts of squalane, preferably 2.5 parts;

1-2 parts of cetearyl alcohol, preferably 1.5 parts;

0.5-1.5 parts of shea butter, preferably 1 part;

1-3 parts of chinaroot herb seed oil, preferably 2 parts;

2-3 parts of glycerol stearate citrate, preferably 2.5 parts.

The preparation method of the anti-inflammatory repair cream comprises the following steps:

(1.1) dissolving arginine in deionized water to obtain an arginine aqueous solution;

(1.2) slowly adding carbomer and xanthan gum into the water phase, and uniformly dispersing to obtain a middle liquid 1;

(1.3) adding an oil phase into the intermediate liquid 1, and homogenizing to obtain an intermediate liquid 2;

(1.4) adding the arginine aqueous solution into the intermediate solution 2, and continuing stirring for 5min to obtain an intermediate solution 3;

and (1.5) adding the nano liposome into the intermediate solution 3 to obtain the anti-inflammatory repair cream.

The time for adding the oil phase into the intermediate liquid 1 in the step (1.3) is that the temperature of the intermediate liquid 1 is 85 ℃;

the preparation method of the oil phase in the step (1.3) comprises the following steps: mixing caprylic/capric triglyceride, squalane, cetostearyl alcohol, shea butter, white chinlon oil, and glyceryl stearate citrate uniformly, and heating to 85 deg.C to obtain oil phase.

The arginine aqueous solution in the step (1.4) is added into the intermediate solution 2 at the time when the temperature of the intermediate solution 2 is 60 ℃.

The time for adding the nano liposome into the intermediate liquid 3 in the step (1.5) is that the temperature of the intermediate liquid 3 is 50 ℃.

The invention also provides anti-inflammatory repair emulsion which comprises the following components in percentage by mass:

water phase: oil phase: carbomer: xanthan gum: arginine: deionized water: the nanoliposome is 82-84: 8-10: 0.1-0.2: 0.1-0.14: 0.1-0.2: 4-6: 1.5-2.5, and preferably comprises an aqueous phase: oil phase: carbomer: xanthan gum: arginine: deionized water: the nano-liposome is 83:9:0.15:0.12:0.15:5: 2;

the water phase comprises the following components in parts by weight: 74-76 parts of deionized water, preferably 75 parts;

3-5 parts of glycerol, preferably 2.5 parts;

2-4 parts of 1, 3-butanediol, preferably 3 parts;

0.4-0.6 part of 1, 2-hexanediol, preferably 0.5 part;

0.4-0.6 part of p-hydroxyacetophenone, preferably 0.5 part.

The oil phase comprises the following components in parts by mass: 2-4 parts of caprylic/capric triglyceride, preferably 3 parts;

0.5-1.5 parts of squalane, preferably 1 part;

0.4-0.6 part of cetearyl alcohol, preferably 0.5 part;

0.5-1.5 parts of shea butter, preferably 1 part;

1-2 parts of chinaroot herb seed oil, preferably 1.5 parts;

1.5-2.5 parts of glycerol stearate citrate, preferably 2 parts.

The preparation method of the anti-inflammatory repair milk comprises the following steps:

(2.1) dissolving arginine in deionized water to obtain an arginine aqueous solution;

(2.2) slowly adding carbomer and xanthan gum into the water phase, and uniformly dispersing to obtain a middle liquid 1;

(2.3) adding an oil phase into the intermediate liquid 1, and homogenizing to obtain an intermediate liquid 2;

(2.4) adding the arginine aqueous solution into the intermediate solution 2, and continuing stirring for 5min to obtain an intermediate solution 3;

and (2.5) adding the nano liposome into the intermediate liquid 3 to obtain the anti-inflammatory repair emulsion.

The time when the oil phase is added into the intermediate liquid 1 in the step (2.3) is that the temperature of the intermediate liquid 1 is 85 ℃;

the preparation method of the oil phase in the step (2.3) comprises the following steps: mixing caprylic/capric triglyceride, squalane, cetostearyl alcohol, shea butter, white chinlon oil, and glyceryl stearate citrate uniformly, and heating to 85 deg.C to obtain oil phase.

And (3) adding the arginine aqueous solution into the intermediate solution 2 at the time when the temperature of the intermediate solution 2 is 60 ℃.

And (3) adding the nano liposome into the intermediate solution 3 in the step (2.5) when the temperature of the intermediate solution 3 is 50 ℃.

An anti-inflammatory repair spray comprises the following components in percentage by mass:

water phase: carbomer: xanthan gum: arginine: deionized water: the nanoliposome is 92-93: 0.05-0.15: 4-6: 1.5-2.5, and preferably is an aqueous phase: carbomer: xanthan gum: arginine: deionized water: the nano-liposome is 92.5:0.1:0.1:0.1:5: 2;

the water phase comprises the following components in parts by weight: 85-86 parts of deionized water, preferably 85.5 parts; 3-5 parts of glycerol, preferably 3.5 parts;

2-4 parts of 1, 3-butanediol, preferably 3 parts;

0.4-0.6 part of 1, 2-hexanediol, preferably 0.5 part;

0.4-0.6 part of p-hydroxyacetophenone, preferably 0.5 part.

The preparation method of the anti-inflammatory repair spray comprises the following steps:

(3.1) dissolving arginine in deionized water to obtain an arginine aqueous solution;

(3.2) slowly adding carbomer and xanthan gum into the water phase, and uniformly dispersing to obtain a middle liquid 1;

(3.3) adding an arginine aqueous solution into the intermediate solution 1 to obtain an intermediate solution 2;

and (3.4) adding the nano liposome into the intermediate liquid 2 to obtain the anti-inflammatory repair spray.

The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.

The mouse macrophage cell line RAW264.7 used in this experimental example was derived from the cell resource center of the institute of basic medicine of Chinese academy of medical sciences.

The human keratinocytes used in this experimental example were obtained from the institute of biotechnology, Beijing Beinanna Chuanglian union.

Example 1

Preparation of nanoliposomes

59.345g of deionized water is added into an emulsifying pot, 0.15g of 150 kilodalton sodium hyaluronate, 0.5g of 40 kilodalton sodium hyaluronate, 0.05g of 0.5 kilodalton sodium hyaluronate and 1g of glycerol are added while stirring, after the temperature is heated to 80 ℃, the mixture is homogenized for 3min under the conditions that the rotating speed is 2000r/min and the vacuum degree is-0.03 mpa, and the phase A is obtained.

When the temperature of phase A is reduced to 70 ℃, 2g of 1, 2-pentanediol, 0.001g of quaternary ammonium salt-73, 2g D-panthenol, 0.1g of beta-glucan, 2.5g of nicotinamide and 1g of hydrogenated lecithin are added, and first homogenization is carried out under the conditions of 6000rpm/min and the vacuum degree of-0.03 mpa for 30 min. And carrying out secondary homogenization under the condition of 60MPa for 30s, and repeating the secondary homogenization for 6 times to obtain the nano liposome for later use.

Preparation of anti-inflammatory repair cream

Slowly adding 0.3g of carbomer and 0.15g of xanthan gum into the water phase at 25 ℃, and uniformly dispersing to obtain intermediate solution 1. Heating the intermediate solution 1 to 85 deg.C, adding the treated oil phase into the intermediate solution 1, and homogenizing for 5min to obtain intermediate solution 2. And when the temperature of the intermediate solution 2 is reduced to 60 ℃, adding the arginine aqueous solution into the intermediate solution 2, and continuously stirring for 5min to obtain an intermediate solution 3. And (3) when the temperature of the intermediate liquid 3 is reduced to 50 ℃, adding 2g of nano liposome, and continuously stirring to 25 ℃ to obtain the anti-inflammatory repair cream.

The water phase comprises: 70.2g of deionized water, 4g of glycerol, 4.5g of 1, 3-butanediol, 0.05g of EDTA-2Na, 0.5g of 1, 2-hexanediol and 0.5g of p-hydroxyacetophenone.

The oil phase treatment method comprises the following steps: uniformly mixing 3g of caprylic/capric triglyceride, 2.5g of squalane, 1.5g of cetostearyl alcohol, 1g of shea butter, 2g of meadowfoam seed oil and 2.5g of glyceryl stearate citrate, and heating to 85 ℃ to obtain the treated oil phase.

The preparation method of the arginine aqueous solution comprises the following steps: 0.3g arginine was dissolved in 5g deionized water.

15 subjects were selected, and 2X 2cm of the forearm curvature of the subject was selected as the test area. After cleaning the skin, a soybean-sized anti-inflammatory repair cream was applied to the test area, and the moisture content in the stratum corneum of the test area was measured before use and at 0h, 4h and 8h after use using a Corneometer (skin moisture tester), respectively. The same site was measured 5 times to exclude the maximum and minimum and then 3 times of average value was recorded, and the measurement results are shown in Table 1.

Example 2

Preparation of nanoliposomes

Adding 73g of deionized water into an emulsifying pot, adding 0.3g of 150 kilodalton sodium hyaluronate, 0.25g of 40 kilodalton sodium hyaluronate, 0.02g of 0.5 kilodalton sodium hyaluronate and 15g of glycerol while stirring, heating to 85 ℃, and homogenizing for 2min under the conditions of a rotating speed of 3000r/min and a vacuum degree of-0.07 mpa to obtain the phase A.

When the temperature of phase A is reduced to 65 ℃, 5g of 1, 2-pentanediol, 0.003g of quaternary ammonium salt-73, 0.4g D-panthenol, 0.001g of beta-glucan, 2g of nicotinamide and 5g of hydrogenated lecithin are added, and first homogenization is carried out under the conditions of 10000rpm/min and vacuum degree of-0.07 mpa for 20 min. And carrying out secondary homogenization under the condition of 100MPa for 45s, and repeating the secondary homogenization for 3 times to obtain the nano liposome for later use.

Preparation of anti-inflammatory repair milk

Slowly adding 0.15g of carbomer and 0.12g of xanthan gum into the water phase at 25 ℃, and uniformly dispersing to obtain intermediate solution 1. Heating the intermediate solution 1 to 85 deg.C, adding the treated oil phase into the intermediate solution 1, and homogenizing for 5min to obtain intermediate solution 2. And when the temperature of the intermediate solution 2 is reduced to 60 ℃, adding the arginine aqueous solution into the intermediate solution 2, and continuously stirring for 5min to obtain an intermediate solution 3. And (3) when the temperature of the intermediate liquid 3 is reduced to 50 ℃, adding 2g of nano liposome, and continuously stirring to 25 ℃ to obtain the anti-inflammatory repair emulsion.

The water phase comprises: 75.58g of deionized water, 4g of glycerol, 3g of 1, 3-butanediol, 0.5g of 1, 2-hexanediol and 0.5g of p-hydroxyacetophenone.

The oil phase treatment method comprises the following steps: uniformly mixing 3g of caprylic/capric triglyceride, 1g of squalane, 0.5g of cetostearyl alcohol, 1g of shea butter, 1.5g of meadowfoam seed oil and 2g of glyceryl stearate citrate, and heating to 85 ℃ to obtain the treated oil phase.

The preparation method of the arginine aqueous solution comprises the following steps: 0.15g arginine was dissolved in 5g deionized water.

Human keratinocytes were used to conduct an in vitro cell assay to examine the effect of the anti-inflammatory repair milk on the release of inflammatory mediators interleukin-1 alpha (1L-1 alpha) and interleukin-8 (1L-8). The method comprises the following specific steps: mixing the anti-inflammatory repair milk with human keratinocyte at a ratio of 2.5:1, and subjecting to UV300mJ/cm²Irradiating for 6min, detecting release amount of 1L-1 alpha and 1L-8, and measuring the release amount of keratinocyte with no anti-inflammatory repair milk added by UV300mJ/cm²The release amounts of 1L-1 alpha and 1L-8 after 6min irradiation are blank controls. The results are shown in Table 2.

Example 3

Preparation of nanoliposomes

94.178g of deionized water is added into an emulsifying pot, 0.2g of 150 kilodalton sodium hyaluronate, 0.6g of 40 kilodalton sodium hyaluronate, 0.03g of 0.5 kilodalton sodium hyaluronate and 25g of glycerol are added while stirring, after the temperature is heated to 82 ℃, the mixture is homogenized for 4min under the conditions that the rotating speed is 2500r/min and the vacuum degree is-0.05 mpa, and the phase A is obtained.

When the temperature of phase A is reduced to 60 ℃, 3g of 1, 2-pentanediol, 0.005g of quaternary ammonium salt-73, 1g D-panthenol, 0.2g of beta-glucan, 1g of nicotinamide and 3g of hydrogenated lecithin are added, and first homogenization is carried out under the conditions of 30000rpm/min and the vacuum degree of-0.05 mpa, and the homogenization time is 5 min. And carrying out secondary homogenization under the condition of 150MPa for 40s to obtain the nano liposome for later use.

Preparation of anti-inflammatory repair spray

Slowly adding 0.1g of carbomer and 0.1g of xanthan gum into the water phase at 25 ℃, and uniformly dispersing to obtain intermediate solution 1. Adding an arginine aqueous solution into the intermediate solution 1, uniformly mixing, adding the nano liposome, and uniformly mixing to obtain the anti-inflammatory repair spray.

The water phase comprises: 85.7g of deionized water, 3g of glycerol, 3g of 1, 3-butanediol, 0.5g of 1, 2-hexanediol and 0.5g of p-hydroxyacetophenone.

The preparation method of the arginine aqueous solution comprises the following steps: 0.1g arginine was dissolved in 5g deionized water.

A mouse peritoneal mast cell culture is taken, 10mL of anti-inflammatory repair spray is used for culturing the cell culture for 25min, and the influence of the anti-inflammatory repair spray on the histamine release amount is detected. The amount of histamine released by the cell culture without any medium added was used as a blank. The results are shown in Table 3.

Comparative example 1

The method of comparative example 1 was set up as in example 1, but no nanoliposomes were added when setting up the anti-inflammatory repair cream of comparative example 1. The effect of the anti-inflammatory repair cream of comparative example 1 on the moisture content of stratum corneum was examined according to the method of example 1. The results are shown in Table 1.

TABLE 1 Effect of the anti-inflammatory repair creams of example 1 and comparative example 1 on moisture content of stratum corneum

Table 1 shows that, after the anti-inflammatory repair cream composed of nanoliposomes is added in example 1, the moisturizing effect is better than that of the anti-inflammatory repair cream composed of nanoliposomes is not added. The nano liposome of the invention has good water replenishing effect.

Comparative example 2

The method of comparative example 2 was set up as in example 2, but no nanoliposomes were added when setting up the anti-inflammatory repair emulsion of comparative example 2. The effect of the anti-inflammatory repair milk of comparative example 2 on the release of inflammatory mediators interleukin-1 alpha (1L-1 alpha), interleukin-8 (1L-8) was examined according to the method of example 2. The results are shown in Table 2.

Table 2 the effect of the anti-inflammatory repair milks of example 2 and comparative example 2 on the release of the inflammatory mediator interleukin

Table 2 shows that the anti-inflammatory prosthetic emulsion of example 2 to which nanoliposomes were added has a significantly improved inhibition rate in inhibiting inflammatory mediators 1L-1 alpha and 1L-8, as compared to the anti-inflammatory prosthetic emulsion of comparative example 2 to which no nanoliposomes were added. Example 2 the anti-inflammatory repair emulsion obtained by adding the nano liposome can reach 76.9 percent of inhibition rate of 1L-1 alpha of inflammatory medium and 68.9 percent of inhibition rate of 1L-8. The nano liposome of the invention can effectively inhibit inflammatory factors.

Comparative example 3

The method of this comparative example 3 was set up as in example 3, but no nanoliposomes were added when setting up the anti-inflammatory repair spray of this comparative example 3. The effect of the anti-inflammatory repair spray of comparative example 3 on histamine release was examined as in example 3. The results are shown in Table 3.

Table 3 effect of anti-inflammatory repair sprays on histamine release in example 3 and comparative example 3

Table 3 shows that the inhibition rate of histamine by the anti-inflammatory repair spray of example 3 prepared by adding nanoliposomes can reach 76.6%, while the inhibition rate of histamine by the anti-inflammatory repair spray of comparative example 3 prepared without adding nanoliposomes is only 20.9%. It is demonstrated that the nanoliposome of the present invention can better inhibit the release of histamine.

Experimental example 1

Adjusting RAW264.7 cell density to 5 × 10⁴Perml, inoculated in a 96-well cell culture plate at 1000. mu.L/well, incubated at 37 ℃ with 5% CO₂Culturing in a cell culture box for 24 h. After the culture was completed, the medium in the cell plate was aspirated, and serum-free cultures containing the nanoliposome of example 1, the anti-inflammatory repair cream of example 2, and the anti-inflammatory repair spray of example 3 at 500. mu.g/mL were added to the treated groupAbout 200. mu.L of the total amount. The blank control group was added with an equal volume of serum-free medium and cultured for 24h, with 3 duplicate wells per group. After 24h, adding 20 mu L of MTT solution into each group, continuously culturing for 4h, taking out a 96-well plate, sucking out liquid in the hole, adding 150 mu L of dimethyl sulfoxide, oscillating for 5min, measuring the absorbance (A) value at 490nm, and calculating the survival rate of the cells. Survival rate is mean a value of drug/mean a value of blank control. The results are shown in Table 4.

TABLE 4 Effect of treatment groups on cell survival

Table 4 shows that the nanoliposome, anti-inflammatory repair cream and anti-inflammatory repair spray obtained by the present invention are safe and mild without any cytotoxicity.

Experimental example 2

Adjusting RAW264.7 cell density to 5 × 10⁴Perml, inoculated in a 96-well cell culture plate at 1000. mu.L/well, incubated at 37 ℃ with 5% CO₂Culturing in a cell culture box for 24 h. After the completion of the culture, the medium in the cell plate was aspirated, 200. mu.L of a serum-free medium containing 100. mu.g/mL of the nanoliposome of example 1 and 1. mu.g/mL of LPS was added to treatment group 1, 200. mu.L of a serum-free medium containing 100. mu.g/mL of the anti-inflammatory repair cream of example 1 and 1. mu.g/mL of LPS was added to treatment group 2, 200. mu.L of a serum-free medium containing 100. mu.g/mL of the anti-inflammatory repair cream of example 2 and 1. mu.g/mL of LPS was added to treatment group 3, 200. mu.L of a serum-free medium containing 100. mu.g/mL of the anti-inflammatory repair spray of example 3 and 1. mu.g/mL of LPS was added to treatment group 4, and an equal volume of the serum-free medium was used as a blank control. After further culturing for 18h, cell supernatants were collected, the content of PGE2 was measured by ELISA kit, and the inhibition rate was calculated, and the results are shown in table 5.

TABLE 5 Effect of treatment groups on PGE2 release from LPS-stimulated cells

Group of	Inhibition ratio (%)
		Nanoliposomes of example 1	94.6
Anti-inflammatory repair cream of example 1	92.4
		Anti-inflammatory repair milk of example 2	93.2
Anti-inflammatory repair spray of example 3	92.8
		Blank control group	32.3

Table 5 shows that the nanoliposome of the present invention and the anti-inflammatory repair cream, anti-inflammatory repair cream and anti-inflammatory repair spray prepared by using the nanoliposome have good ability of inhibiting LPS from stimulating cells to release PGE 2. The nano-liposome of the invention has good effect of inhibiting PGE2 inflammatory factors.

Experimental example 3

The effect of the nanoliposomes of example 1, the anti-inflammatory repair cream of example 1, the anti-inflammatory repair milk of example 2, and the anti-inflammatory repair spray of example 3 on the release of TNF-a by LPS-stimulated RAW264.7 cells was examined as in experimental example 2. The results are shown in Table 6.

TABLE 6 Effect of treatment groups on TNF-a release from LPS stimulated cells

Group of	Inhibition ratio (%)
		Nanoliposomes of example 1	92.1
Anti-inflammatory repair cream of example 1	90.6
		Anti-inflammatory repair milk of example 2	91.5
Anti-inflammatory repair spray of example 3	90.8
		Blank control group	34.1

Table 6 shows that the nanoliposome of the present invention and the anti-inflammatory repair cream, and the anti-inflammatory repair spray prepared by using the nanoliposome have excellent ability of inhibiting LPS from stimulating cells to release TNF-a. The nano-liposome of the invention has good function of inhibiting TNF-a inflammatory factors.

From the above embodiments, the present invention provides a nanoliposome, a preparation method and an application thereof, and an anti-inflammatory repair cream, an anti-inflammatory repair cream and an anti-inflammatory repair spray, and the nanoliposome of the present invention has the following advantages:

(1) the invention preferably selects three kinds of transparent materials with different molecular weightsThe organic combination of the sodium hyaluronate exerts the synergistic effect of hyaluronic acid with different molecular weights on the inhibition of inflammatory reaction and the promotion of repair. And because the transdermal properties of the nicotinamide and the D-panthenol are good, part of the cationic active substances, namely the quaternary ammonium salt-73, the D-panthenol and the nicotinamide, act on the epidermal layer and the dermal layer together to achieve better sterilization and bacteriostasis effects. Conversion of nicotinamide to NDA in skin cells⁺And NDAP⁺And (3) the enzyme is supplemented, so that a DNA repair mechanism is improved, structural protein, lipid and collagen of a dermis layer of an epidermis layer are stimulated, and the cell structure is gradually restored to be complete.

(2) The nano liposome prepared by the preparation method can improve the transdermal absorption level and the sustained release capability and can better exert the effect.

(3) The nano-liposome has obvious inhibition effect on inflammatory factors, has long-acting anti-inflammatory effect and can fundamentally repair skin barriers.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.