阅读说明：本技术 一种用于光片和结构光显微镜成像的通用耦合槽 (Universal coupling groove for imaging of light sheet and structured light microscope ) 是由 顾忠泽 陈早早 李奇维 徐世慧 胡倩倩 程斌 于 2021-06-04 设计创作，主要内容包括：本发明公开了一种用于光片和结构光显微镜成像的通用耦合槽,包括耦合槽主体和调节耦合槽主体内部液体温度的控制器,所述耦合槽主体的一侧外表面开设有进液管口和出液管口,所述进液管口位于出液管口的上方。本发明所述的一种用于光片和结构光显微镜成像的通用耦合槽,可以自动补水,使水位一直保持在预定的高度,能保证耦合槽内液体的温度,使得显微镜在较长时间观察活体样本成像时,保证样品的活性环境,解决人力补水费时费力的问题,能够对适配管状、玻片、微流芯片和孔板多种样本装载及夹持,使用范围比较广,而且能够进行快速三维成像,显微镜成像的效率和质量高,还能完全排干净耦合槽的水,方便人们清理,而且具有紫外线杀菌功能。(The invention discloses a general coupling groove for imaging of a light sheet and a structured light microscope, which comprises a coupling groove main body and a controller for adjusting the temperature of liquid in the coupling groove main body, wherein the outer surface of one side of the coupling groove main body is provided with a liquid inlet pipe orifice and a liquid outlet pipe orifice, and the liquid inlet pipe orifice is positioned above the liquid outlet pipe orifice. The general coupling groove for imaging of the light sheet and the structured light microscope can automatically replenish water, keep the water level at a preset height all the time, ensure the temperature of liquid in the coupling groove, ensure the active environment of a sample when the microscope observes the imaging of a living body sample for a long time, solve the problem that the manual water replenishing wastes time and labor, load and clamp various samples of an adaptive tubular shape, a glass sheet, a micro-flow chip and a pore plate, have a wide application range, can perform rapid three-dimensional imaging, have high imaging efficiency and quality of the microscope, can completely drain the water in the coupling groove, is convenient for people to clean, and has an ultraviolet sterilization function.)

1. A universal coupling slot for imaging of optical sheet and structured light microscope is characterized by comprising

Coupling groove main part (1) and controller (2) that are used for adjusting the inside liquid temperature of coupling groove main part (1), the feed liquor mouth of pipe (3) and play liquid mouth of pipe (4) have been seted up to one side surface of coupling groove main part (1), feed liquor mouth of pipe (3) are located the top of play liquid mouth of pipe (4), the inlet of controller (2) passes through the pipeline and communicates with play liquid mouth of pipe (4), be provided with U type linker (5) on feed liquor mouth of pipe (3), the liquid outlet of controller (2) passes through pipeline and U type linker (5) intercommunication, be provided with photoelectric switch (6) that are used for responding to coupling groove main part (1) liquid level on U type linker (5).

2. The universal coupling slot for light sheet and structured light microscope imaging of claim 1, wherein: the shape of coupling groove main part (1) is the triangular prism, the both sides surface of coupling groove main part (1) is close to lower extreme position and has all been seted up little objective mouth (7), the shape of little objective mouth (7) is cylindrical.

3. The universal coupling slot for light sheet and structured light microscope imaging of claim 2, wherein: the front end surface of coupling groove main part (1) is seted up and is used for inserting cylinder sample mouth (8) of adaptation tubulose sample or ultraviolet sterilization disinfection stick (10), the internal surface of cylinder sample mouth (8) is provided with the sealing washer, orifice plate sample mouth (9) have been seted up to the upper end surface of coupling groove main part (1).

4. The universal coupling slot for light sheet and structured light microscope imaging according to claim 3, wherein: the ultraviolet sterilization disinfection rod (10) is provided with a plurality of groups of LED ultraviolet lamp groups.

5. The universal coupling slot for light sheet and structured light microscope imaging according to claim 3, wherein: a slide is arranged on the sample port (9) of the pore placing plate.

6. The universal coupling slot for light sheet and structured light microscope imaging according to claim 3, wherein: and a microfluidic chip is arranged on the sample port (9) of the pore-placing plate.

7. The universal coupling slot for light sheet and structured light microscope imaging according to claim 3, wherein: the sample port (9) of the pore plate is provided with a pore plate placing rack, the pore plate placing rack is provided with a pore plate, and the U-shaped bottom of the pore plate is positioned inside the coupling groove main body (1).

Technical Field

The invention relates to the field of light microscope, in particular to a universal coupling groove for imaging of a light sheet and a structured light microscope.

Background

The microscope has the characteristics of non-contact, no damage and capability of detecting the internal information of a sample, so that the microscope is called as a most common exploration tool in the field of life science, particularly the research field of living cell life processes. With the continuous and deep research, the requirements for the microscope breaking through the diffraction limit are more and more urgent. Through continuous efforts of scientists, the technologies of the light-sheet microscope and the structured light microscope are more and more mature, and the advantages of the two microscopes such as a confocal microscope and a two-photon microscope for observing the rapid and high-resolution imaging of living cells are very obvious, so that the design of a universal coupling groove which can be used for the light-sheet microscope and the structured light microscope is very necessary, and therefore, the universal coupling groove for imaging of the light-sheet microscope and the structured light microscope is provided.

Disclosure of Invention

The invention mainly aims to provide a general coupling groove for imaging of a light sheet and a structured light microscope, which can effectively solve the problems in the background art.

In order to achieve the purpose, the invention adopts the technical scheme that:

the utility model provides a general coupling groove for slide and formation of image of structure light microscope, includes the coupling groove main part and is used for adjusting the controller of the inside liquid temperature of coupling groove main part, the feed liquor mouth of pipe and the play liquid mouth of pipe have been seted up to one side surface of coupling groove main part, the feed liquor mouth of pipe is located a liquid orificial top, the inlet of controller passes through the pipeline and communicates with a liquid mouth of pipe, be provided with U type linker on the feed liquor mouth of pipe, the liquid outlet of controller passes through pipeline and U type linker intercommunication, be provided with the photoelectric switch who is used for responding to coupling groove main part liquid level on the U type linker.

Preferably, the coupling groove main body is triangular prism, the positions of the outer surfaces of the two sides of the coupling groove main body, which are close to the lower end, are provided with the microscope openings, and the microscope openings are cylindrical.

Preferably, the front end outer surface of the coupling groove main body is provided with a cylindrical sample port for inserting an adaptive tubular sample or an ultraviolet sterilization disinfection rod, the inner surface of the cylindrical sample port is provided with a sealing ring, and the upper end outer surface of the coupling groove main body is provided with a hole plate sample port.

Preferably, a plurality of groups of LED ultraviolet lamp groups are arranged on the ultraviolet sterilization and disinfection rod.

Preferably, a slide is arranged on the sample port of the pore placing plate.

Preferably, a microfluidic chip is arranged on the sample port of the pore plate.

Preferably, a pore plate placing frame is arranged on the pore plate sample port, a pore plate is arranged on the pore plate placing frame, and the U-shaped bottom of the pore plate is positioned inside the coupling groove main body.

Compared with the prior art, the invention provides a universal coupling groove for imaging of an optical sheet and a structured light microscope, which has the following beneficial effects:

1. through the photoelectric switch arranged on the U-shaped communicating vessel, when the position of the liquid descends, the photoelectric switch turns on the controller, and the liquid is guided in by the controller, so that the controller can automatically replenish water, and the water level is always kept at a preset height;

2. through the controller connected with the coupling groove, the controller can output constant-temperature liquid at 37 ℃, so that the temperature of the liquid in the coupling groove is ensured, the active environment of a sample is ensured when a microscope observes the imaging of a living body sample for a long time, and the problems that manual water supplement wastes time and labor are solved;

3. the coupling groove can load and clamp various samples of adaptive tubular, glass slide, microfluidic chip and pore plate, has a wide application range, can perform rapid three-dimensional imaging, and has high imaging efficiency and quality of a microscope;

4. the shape of coupling groove is the triangular prism, and the play liquid mouth of pipe sets up the side bottom position at the coupling groove, can completely exhaust the water of clean coupling groove, makes things convenient for people to clear up, has used up and can insert the ultraviolet sterilization disinfection stick and carry out ultraviolet sterilization, and is more convenient, and the whole general coupling groove that is used for slide and structure light microscope formation of image's structure is simpler moreover, and the operation is more convenient, and the reliability is high, and the precision is high, and is with low costs, comparatively practical.

The parts of the device not involved are the same as or can be implemented using prior art.

Drawings

FIG. 1 is a schematic diagram of the overall structure of a universal coupling slot for imaging with a light sheet and a structured light microscope according to the present invention;

FIG. 2 is a diagram of a coupling slot main body in a universal coupling slot for imaging with an optical sheet and a structured light microscope according to the present invention;

FIG. 3 is a diagram of the internal structure of the coupling slot main body in the universal coupling slot for imaging the optical sheet and the structured light microscope according to the present invention;

FIG. 4 is a diagram showing the positional relationship between a coupling groove and a microscope stage in a general coupling groove for imaging an optical sheet and a structured light microscope according to the present invention;

FIG. 5 is a diagram of a hole plate holder in a universal coupling slot for imaging an optical sheet and a structured light microscope according to the present invention;

FIG. 6 is a schematic diagram of the communication between the light path of the light sheet microscope and the objective table in the universal coupling slot for imaging the light sheet and the structured light microscope according to the present invention;

FIG. 7 is a schematic diagram of the communication between the objective table and the structured light microscope in the universal coupling slot for imaging the light sheet and the structured light microscope according to the present invention;

FIG. 8 is a diagram of the structure of the UV disinfection bar in the universal coupling slot for imaging with the slide and structured light microscope of the present invention.

In the figure: 1. a coupling groove main body; 2. a controller; 3. a liquid inlet pipe orifice; 4. a liquid outlet pipe orifice; 5. a U-shaped communicating vessel; 6. a photoelectric switch; 7. a microscopic objective lens opening; 8. a cylindrical sample port; 9. placing a sample port of the orifice plate; 10. an ultraviolet sterilization disinfection bar.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.

Example 1

The utility model provides a general coupling groove for slide and structure light microscope formation of image, as shown in fig. 1-8, including coupling groove main part 1 and the controller 2 that is used for adjusting the inside liquid temperature of coupling groove main part 1, the feed liquor mouth of pipe 3 and the play liquid mouth of pipe 4 have been seted up to one side surface of coupling groove main part 1, feed liquor mouth of pipe 3 is located the top of play liquid mouth of pipe 4, the inlet of controller 2 passes through the pipeline and communicates with play liquid mouth of pipe 4, be provided with U type linker 5 on the feed liquor mouth of pipe 3, the liquid outlet of controller 2 passes through pipeline and U type linker 5 intercommunication, be provided with photoelectric switch 6 that is used for responding to 1 liquid level in coupling groove main part on the U type linker 5.

The shape of coupling groove main part 1 is the triangular prism, and micro objective mouth 7 has all been seted up near lower extreme position to the both sides surface of coupling groove main part 1, and micro objective mouth 7's shape is cylindrical.

The front end surface of coupling groove main part 1 is seted up and is used for inserting adaptation tubulose sample or ultraviolet sterilization disinfection stick 10's cylinder sample mouth 8, and the internal surface of cylinder sample mouth 8 is provided with the sealing washer, and the upper end surface of coupling groove main part 1 has been seted up and has been put orifice plate sample mouth 9, and 8 inside adaptations tubulose samples that are provided with of cylinder sample mouth.

When imaging the adaptation tubular sample, insert the adaptation tubular sample in cylinder sample mouth 8, adaptation tubular sample and sealing washer contact prevent that coupling groove liquid from leaking.

Example 2

The utility model provides a general coupling groove for slide and formation of image of structure light microscope, including coupling groove main part 1 with be used for adjusting coupling groove main part 1 inside liquid temperature's controller 2, the feed liquor mouth of pipe 3 and the play liquid mouth of pipe 4 have been seted up to one side surface of coupling groove main part 1, feed liquor mouth of pipe 3 is located the top of play liquid mouth of pipe 4, the inlet of controller 2 passes through pipeline and the 4 intercommunication of play liquid mouth of pipe, be provided with U type linker 5 on the feed liquor mouth of pipe 3, the liquid outlet of controller 2 passes through pipeline and 5 intercommunications of U type linker, be provided with the photoelectric switch 6 that is used for responding to 1 liquid level in coupling groove main part on the U type linker 5.

The shape of coupling groove main part 1 is the triangular prism, and micro objective mouth 7 has all been seted up near lower extreme position to the both sides surface of coupling groove main part 1, and micro objective mouth 7's shape is cylindrical.

The front end surface of coupling groove main part 1 is seted up and is used for inserting the cylinder sample mouth 8 of adaptation tubulose sample or ultraviolet sterilization disinfection stick 10, and the internal surface of cylinder sample mouth 8 is provided with the sealing washer, and the upper end surface of coupling groove main part 1 has been seted up and has been put orifice plate sample mouth 9, is provided with the slide on the orifice plate sample mouth 9.

Example 3

The utility model provides a general coupling groove for slide and formation of image of structure light microscope, including coupling groove main part 1 with be used for adjusting coupling groove main part 1 inside liquid temperature's controller 2, the feed liquor mouth of pipe 3 and the play liquid mouth of pipe 4 have been seted up to one side surface of coupling groove main part 1, feed liquor mouth of pipe 3 is located the top of play liquid mouth of pipe 4, the inlet of controller 2 passes through pipeline and the 4 intercommunication of play liquid mouth of pipe, be provided with U type linker 5 on the feed liquor mouth of pipe 3, the liquid outlet of controller 2 passes through pipeline and 5 intercommunications of U type linker, be provided with the photoelectric switch 6 that is used for responding to 1 liquid level in coupling groove main part on the U type linker 5.

The shape of coupling groove main part 1 is the triangular prism, and micro objective mouth 7 has all been seted up near lower extreme position to the both sides surface of coupling groove main part 1, and micro objective mouth 7's shape is cylindrical.

The front end surface of coupling groove main part 1 is seted up and is used for inserting adaptation tubulose sample or ultraviolet sterilization disinfection stick 10's cylinder sample mouth 8, and the internal surface of cylinder sample mouth 8 is provided with the sealing washer, and the upper end surface of coupling groove main part 1 has been seted up and has been put orifice plate sample mouth 9, is provided with the miniflow chip on the orifice plate sample mouth 9.

Example 4

The utility model provides a general coupling groove for slide and formation of image of structure light microscope, including coupling groove main part 1 with be used for adjusting coupling groove main part 1 inside liquid temperature's controller 2, the feed liquor mouth of pipe 3 and the play liquid mouth of pipe 4 have been seted up to one side surface of coupling groove main part 1, feed liquor mouth of pipe 3 is located the top of play liquid mouth of pipe 4, the inlet of controller 2 passes through pipeline and the 4 intercommunication of play liquid mouth of pipe, be provided with U type linker 5 on the feed liquor mouth of pipe 3, the liquid outlet of controller 2 passes through pipeline and 5 intercommunications of U type linker, be provided with the photoelectric switch 6 that is used for responding to 1 liquid level in coupling groove main part on the U type linker 5.

The shape of coupling groove main part 1 is the triangular prism, and micro objective mouth 7 has all been seted up near lower extreme position to the both sides surface of coupling groove main part 1, and micro objective mouth 7's shape is cylindrical.

The cylinder sample mouth 8 that is used for inserting adaptation tubulose sample or ultraviolet sterilization disinfection stick 10 is seted up to the front end surface of coupling groove main part 1, and the internal surface of cylinder sample mouth 8 is provided with the sealing washer, and the upper end surface of coupling groove main part 1 has been seted up and has been put orifice plate sample mouth 9, is provided with the orifice plate rack on the orifice plate sample mouth 9, is provided with the orifice plate on the orifice plate rack, and the U type bottom of orifice plate is located inside coupling groove main part 1.

When imaging is carried out on a sample in the pore plate, the sample placing hole of the cylinder is sealed by the sealing cover, the pore plate is placed on the pore plate placing frame at first, then the placing frame is placed on the microscope stage, the microscope stage is positioned above the coupling groove, and as shown in fig. 6, the U-shaped bottom of the pore plate is just completely immersed in the liquid of the coupling groove main body 1.

The ultraviolet sterilization disinfection rod 10 is provided with a plurality of groups of LED ultraviolet lamp groups.

The invention is a general coupling groove for imaging of a light sheet and a structured light microscope, when the general coupling groove is used, water can be automatically supplemented through a controller 2, so that the water level is always kept at a preset height, the controller 2 can output constant-temperature liquid of 37 ℃, the temperature of the liquid in the coupling groove is ensured, the active environment of a sample is ensured when a microscope observes an imaging of a living body sample for a long time, a liquid inlet pipe opening 3 of the coupling groove is provided with a U-shaped communicating device 5, the outer side of the coupling groove is provided with a photoelectric switch 6, and when the liquid position descends, the photoelectric switch 6 opens the controller 2, and the liquid is introduced into the coupling groove by the controller 2;

a microscope objective is placed through the microscope opening 7, when the light-sheet microscope is used, the light-sheet microscope illuminating objective is placed at the left side microscope opening 7, the light-sheet microscope imaging objective is placed at the right side, and a sealing ring is used at the opening part to prevent liquid in the coupling groove from leaking through the microscope opening 7;

when the structured light microscope is used, the structured light microscope objective is placed in the left side microscope opening 7, and the right side microscope opening 7 is sealed by the sealing cover, so that liquid in the coupling groove is prevented from leaking out through the microscope opening 7;

after the experiment is finished, the coupling liquid is completely discharged through the liquid outlet pipe orifice 4, and then the ultraviolet sterilization disinfection rod 10 is inserted into the cylindrical sample placing orifice 8 for sterilization;

through the photoelectric switch 6 arranged on the U-shaped communicating vessel 5, when the liquid position descends, the photoelectric switch 6 turns on the controller 2, and the liquid is introduced from the controller 2, so that the controller 2 can automatically supplement water, and the water level is always kept at a preset height;

through the controller 2 connected with the coupling groove, the controller 2 can output constant-temperature liquid at 37 ℃, so that the temperature of the liquid in the coupling groove is ensured, the active environment of a sample is ensured when a microscope observes the imaging of a living body sample for a long time, and the problems that manual water supplement wastes time and labor are solved;

the coupling groove can load and clamp various samples of adaptive tubular, glass slide, microfluidic chip and pore plate, has a wide application range, can perform rapid three-dimensional imaging, and has high imaging efficiency and quality of a microscope;

the shape of coupling groove is the triangular prism, and liquid outlet pipe mouth 4 sets up the side bottom position at the coupling groove, can completely exhaust the water of clean coupling groove, makes things convenient for people to clear up, has used up and can insert ultraviolet sterilization disinfection stick 10 and carry out ultraviolet sterilization, and is more convenient, and the whole general coupling groove that is used for slide and structure light microscope formation of image's structure is simpler moreover, and the operation is more convenient, and the reliability is high, and the precision is high, and is with low costs, comparatively practical.

The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.