阅读说明：本技术 一种补钙大鲵骨钙片及其制备方法 (Giant salamander bone calcium tablet for supplementing calcium and preparation method thereof ) 是由 汤斌 杨晓东 蔡志明 于 2021-09-14 设计创作，主要内容包括：本发明公开了一种补钙大鲵骨钙片,包括以下按重量份数配比的原料：大鲵骨粉40份-80份,硫酸软骨素15份-45份,维生素5份-10份,硬脂酸镁0.1份-1份；其中,大鲵骨粉由带软骨的大鲵骨制成,硫酸软骨素由大鲵软骨制备得到,并且大鲵软骨和大鲵骨的质量比为9:1-81。本发明还公开了一种补钙大鲵骨钙片的制备方法。本发明大鲵骨钙片的制备工艺简单,并且制备所得的大鲵骨钙片具有钙含量高优点；同时本发明的大鲵在酶解过程中,其软骨不会随着大鲵肉一起被酶解掉,酶解后依然会保留完整的软骨；本发明还为大鲵深加工的开发及其资源化利用提供理论和技术支持,同时对推动大鲵产业在营养和医药方面的发展具有重要意义。(The invention discloses a giant salamander bone calcium tablet for supplementing calcium, which comprises the following raw materials in parts by weight: 40-80 parts of giant salamander bone meal, 15-45 parts of chondroitin sulfate, 5-10 parts of vitamin and 0.1-1 part of magnesium stearate; the giant salamander bone meal is prepared from giant salamander bones with cartilages, the chondroitin sulfate is prepared from the giant salamander cartilages, and the mass ratio of the giant salamander cartilages to the giant salamander bones is 9: 1-81. The invention also discloses a preparation method of the giant salamander bone calcium tablets for supplementing calcium. The preparation process of the giant salamander bone calcium tablet is simple, and the giant salamander bone calcium tablet prepared by the preparation process has the advantage of high calcium content; meanwhile, in the enzymolysis process of the giant salamander, the cartilage of the giant salamander cannot be enzymolyzed away along with the giant salamander meat, and the complete cartilage can still be remained after enzymolysis; the invention also provides theoretical and technical support for the development of giant salamander deep processing and resource utilization thereof, and has important significance for promoting the development of the giant salamander industry in the aspects of nutrition and medicine.)

1. The giant salamander bone calcium tablet for supplementing calcium is characterized by comprising the following raw materials in parts by weight:

40-80 parts of giant salamander bone meal, 15-45 parts of chondroitin sulfate,

5-10 parts of vitamin and 0.1-1 part of magnesium stearate;

the giant salamander bone meal is prepared from giant salamander bones with cartilages, the chondroitin sulfate is prepared from the giant salamander cartilages, and the mass ratio of the giant salamander cartilages to the giant salamander bones is 9: 1-81.

2. The preparation method of the giant salamander bone calcium tablets for supplementing calcium is characterized by comprising the following steps:

step (1), obtaining giant salamander cartilage and giant salamander bone: slaughtering artificially cultured giant salamanders, adding clear water into the bone-carrying part to prepare feed liquid, wherein the ratio of the giant salamander bones to the clear water in the feed liquid is 1:1-10, adding protease and lipolytic enzyme into the feed liquid for enzymolysis, wherein the enzyme adding amount of the protease and the lipolytic enzyme is 1-15 per mill, the enzymolysis temperature is 45-55 ℃, the enzymolysis time is 20-25 min, obtaining the giant salamander cartilage and the giant salamander bones after enzymolysis, and then cleaning the obtained giant salamander cartilage and the giant salamander bones with soft water;

step (2), giant salamander bone meal preparation: directly airing or drying the giant salamander bone with the cartilage, stirring, coarsely grinding, finely grinding and drying to obtain nanoscale giant salamander bone powder;

step (3) preparing a chondroitin sulfate-containing cartilage preparation: adding clear water into the giant salamander cartilage to prepare a feed liquid, wherein the ratio of the giant salamander cartilage to the clear water in the feed liquid is 1:1-10, adding compound protease into the feed liquid for enzymolysis, wherein the enzyme adding amount of the compound protease is 1-5 per mill, the enzymolysis time is 10min-2h, the enzymolysis temperature is 45-55 ℃, and the giant salamander cartilage after enzymolysis is subjected to centrifugation, concentration and drying to obtain a chondroitin sulfate-containing cartilage preparation;

step (4) preparing calcium tablets: weighing 40-80 parts of giant salamander bone meal, 20-50 parts of chondroitin sulfate and 5-10 parts of vitamin according to parts by weight, uniformly mixing, granulating starch slurry, sieving, drying to enable the moisture content of whole particles to reach 4% -5%, adding 0.1-1 part of magnesium stearate, uniformly mixing, and pressing into tablets.

3. The preparation method of the giant salamander bone calcium tablet for supplementing calcium according to claim 2, which is characterized by comprising the following steps: in the step (2), a 100-200 mesh sieve is selected for coarse grinding, a 300-500 mesh sieve is selected for fine grinding, and a 1000-1200 mesh sieve is selected for fine grinding.

4. The preparation method of the giant salamander bone calcium tablet for supplementing calcium according to claim 2, which is characterized by comprising the following steps: and (3) completing the centrifugation in a centrifuge, wherein the rotation speed of the centrifuge is 3000r/min-6000r/min, and the centrifugation time is 3min-8 min.

5. The preparation method of the giant salamander bone calcium tablet for supplementing calcium according to claim 2, which is characterized by comprising the following steps: in the step (3), the concentration temperature is 50-60 ℃, and the concentration pressure is 0.05-0.1 MPa.

6. The preparation method of the giant salamander bone calcium tablet for supplementing calcium according to claim 2, which is characterized by comprising the following steps: in the step (3), the drying is freeze drying or spray drying.

Technical Field

The invention relates to the field of health care products, in particular to a giant salamander bone calcium tablet for supplementing calcium and a preparation method thereof.

Background

The giant salamander belongs to the family of the hidden gilled giant salamanders and belongs to tailed amphibians, is the only large amphibians existing in China and is listed as national second-class protective animals, and meanwhile, the giant salamander is a special species which has comprehensive nutritional value and different efficacy functions at each part. The giant salamander has high economic value, can strengthen physique and can effectively prevent and treat anemia, menstrual disorder and other diseases; the giant salamander has thick, solid and tough skin and can be used for making leather; can also be made into medicine for treating burn; can also be used for treating gastropathy such as infantile hiccup; bile can clear heat and improve eyesight.

The nutritional value of the giant salamander is comprehensively realized in the following aspects: (1) the amino acid content of the giant salamander is complete and the total content is high, so that the giant salamander accords with a chicken protein amino acid mode; (2) the giant salamander has high total protein content and high proportion balance; (3) the content of unsaturated fatty acid of the giant salamander is high, the proportion of the unsaturated fatty acid and the balance degree of the unsaturated fatty acid are good, and the specificity is obvious; (4) the giant salamander contains human body trace elements with good quality, high index and easy absorption; (5) the giant salamander has various active substance contents and unique effect. The giant salamander also has the functions of resisting oxidation, resisting fatigue, resisting immune cell proliferation and tumor, removing toxic substances and delaying aging.

Cartilage is rich in glycoprotein, polypeptide, small molecular protein, and polysaccharide. Research shows that some animal cartilage extracts have good immunoregulation and anti-arthritis effects. The document ICP-AES method determination and analysis of several important mineral substances in giant salamander cartilage and muscle in the Chinese breeding discloses that the content of calcium in the giant salamander cartilage is as high as 493mg/l00g and is greatly higher than 252mg/100g of shark fins (dried) than gold. Like the processing of shark's fin, the giant salamander cartilage can also be processed into delicious food, which provides a valuable reference for the development and utilization of the giant salamander cartilage, especially at present, the current situation of nutrition of residents in China shows that high-absorption rate, high-quality and high-calcium food is in short supply. Undoubtedly, the abundant calcium content of the giant salamander cartilage provides a new clue for the comprehensive development and utilization of the giant salamander.

In the prior art, the cartilage of the giant salamander is usually enzymolyzed together with the giant salamander meat in the enzymolysis process, only the undecomposed giant salamander hard bone is left, and the complete cartilage cannot be reserved.

Disclosure of Invention

The invention aims to provide calcium-supplementing giant salamander bone calcium tablets and a preparation method thereof, and aims to solve the technical problems that cartilage of the giant salamander is usually decomposed by enzyme together with giant salamander meat in an enzymolysis process in the prior art, only undecomposed giant salamander hard bones are left, and complete cartilage cannot be reserved.

In order to achieve the purpose, one embodiment of the invention provides a calcium-supplementing giant salamander bone calcium tablet which comprises the following raw materials in parts by weight:

40-80 parts of giant salamander bone meal, 15-45 parts of chondroitin sulfate,

5-10 parts of vitamin and 0.1-1 part of magnesium stearate;

the giant salamander bone meal is prepared from giant salamander bones with cartilages, the chondroitin sulfate is prepared from the giant salamander cartilages, and the mass ratio of the giant salamander cartilages to the giant salamander bones is 9: 1-81.

Based on the giant salamander bone calcium tablets for supplementing calcium provided by the invention, the invention also discloses a preparation method of the giant salamander bone calcium tablets for supplementing calcium, which comprises the following steps:

step (1), obtaining giant salamander cartilage and giant salamander bone: slaughtering artificially cultured giant salamanders, adding clear water into the bone-carrying part to prepare feed liquid, wherein the ratio of the giant salamander bones to the clear water in the feed liquid is 1:1-10, adding protease and lipolytic enzyme into the feed liquid for enzymolysis, wherein the enzyme adding amount of the protease and the lipolytic enzyme is 1-15 per mill, the enzymolysis temperature is 45-55 ℃, the enzymolysis time is 20-25 min, obtaining the giant salamander cartilage and the giant salamander bones after enzymolysis, and then cleaning the obtained giant salamander cartilage and the giant salamander bones with soft water;

step (2), giant salamander bone meal preparation: directly airing or drying the giant salamander bone with the cartilage, stirring, coarsely grinding, finely grinding and drying to obtain nanoscale giant salamander bone powder;

step (3) preparing a chondroitin sulfate-containing cartilage preparation: adding clear water into the giant salamander cartilage to prepare a feed liquid, wherein the ratio of the giant salamander cartilage to the clear water in the feed liquid is 1:1-10, adding compound protease into the feed liquid for enzymolysis, wherein the enzyme adding amount of the compound protease is 1-5 per mill, the enzymolysis time is 10min-2h, the enzymolysis temperature is 45-55 ℃, and the giant salamander cartilage after enzymolysis is subjected to centrifugation, concentration and drying to obtain a chondroitin sulfate-containing cartilage preparation;

step (4) preparing calcium tablets: weighing 40-80 parts of giant salamander bone meal, 20-50 parts of chondroitin sulfate and 5-10 parts of vitamin according to parts by weight, uniformly mixing, granulating starch slurry, sieving, drying to enable the moisture content of whole particles to reach 4% -5%, adding 0.1-1 part of magnesium stearate, uniformly mixing, and pressing into tablets.

In one preferable scheme of the invention, in the step (2), a 100-200 mesh sieve is selected for coarse grinding, a 300-500 mesh sieve is selected for fine grinding, and a 1000-1200 mesh sieve is selected for fine grinding.

In one preferable scheme of the invention, in the step (3), the centrifugation is finished in a centrifuge, the rotating speed of the centrifuge is 3000r/min-6000r/min, and the centrifugation time is 3min-8 min.

In one preferable scheme of the invention, in the step (3), the concentration temperature is 50-60 ℃, and the concentration pressure is 0.05-0.1 MPa.

In a preferred embodiment of the present invention, in the step (3), the drying is freeze-drying or spray-drying.

In summary, the invention has the following advantages:

1. the preparation process of the giant salamander bone calcium tablet is simple, and the prepared giant salamander bone calcium tablet has the advantages of high calcium content and smooth tablet surface, and is suitable for daily calcium supplement of various crowds.

2. In the enzymolysis process of the giant salamander, the cartilage of the giant salamander cannot be enzymolyzed together with the giant salamander meat, and the complete cartilage can still be remained after enzymolysis.

3. The invention provides theoretical and technical support for the development of giant salamander deep processing and resource utilization thereof, and has important significance for promoting the development of the giant salamander industry in the aspects of nutrition and medicine.

Detailed Description

Example 1

The giant salamander bone calcium tablet for supplementing calcium comprises the following raw materials in parts by weight: 60 parts of giant salamander bone meal, 30 parts of chondroitin sulfate, 7.5 parts of vitamin and 0.55 part of magnesium stearate;

the giant salamander bone meal is prepared from giant salamander bones with cartilages, the chondroitin sulfate is prepared from the giant salamander cartilages, and the mass ratio of the giant salamander cartilages to the giant salamander bones is 1: 1.

A preparation method of calcium supplementing giant salamander bone calcium tablets comprises the following steps:

step (1), obtaining giant salamander cartilage and giant salamander bone: slaughtering artificially cultured giant salamanders, adding clear water into the bone-carrying part to prepare feed liquid, wherein the ratio of the giant salamander bones to the clear water in the feed liquid is 1:1-10, adding protease and lipolytic enzyme into the feed liquid for enzymolysis, wherein the enzyme adding amount of the protease and the lipolytic enzyme is 1-15 per mill, the enzymolysis temperature is 45-55 ℃, the enzymolysis time is 20-25 min, obtaining the giant salamander cartilage and the giant salamander bones after enzymolysis, and then cleaning the obtained giant salamander cartilage and the giant salamander bones with soft water;

step (2), giant salamander bone meal preparation: directly airing or drying the giant salamander bone with the cartilage, stirring, coarsely grinding after stirring, sieving by using a sieve with 100-200 meshes after coarsely grinding, finely grinding after coarsely grinding, sieving by using a sieve with 300-500 meshes after finely grinding, finely grinding after finely grinding, sieving by using a sieve with 1000-1200 meshes after finely grinding, and finally drying to obtain the nanoscale giant salamander bone powder;

step (3) preparing the chondroitin sulfate-containing cartilage preparation, comprising the following steps:

a. enzymolysis: adding clear water into the giant salamander cartilage to prepare a feed liquid, wherein the ratio of the giant salamander cartilage to the clear water in the feed liquid is 1:1-10, adding compound protease into the feed liquid for enzymolysis, wherein the enzyme adding amount of the compound protease is 1-5 per mill, the enzymolysis time is 10min-2h, and the enzymolysis temperature is 45-55 ℃ to prepare an enzymolysis liquid;

b. centrifuging: centrifuging the enzymolysis liquid by a centrifuge at 3000-6000 r/min for 3-8 min;

c. concentration: concentrating the centrifuged supernatant at 50-60 deg.C under 0.05-0.1 MPa;

d. and (3) drying: freeze drying or spray drying; putting the concentrated material into a spray dryer for spray drying treatment to prepare a chondroitin sulfate-containing cartilage preparation;

step (4) preparing calcium tablets: weighing 60 parts of giant salamander bone powder, 30 parts of chondroitin sulfate and 7.5 parts of vitamin according to the following weight parts, uniformly mixing the materials, then granulating starch slurry, sieving the starch slurry by a 100-mesh sieve, drying the starch slurry in an oven at 60 ℃ for 20min to 30min to ensure that the moisture of whole particles reaches 4 to 5 percent, adding 0.55 part of magnesium stearate, uniformly mixing, and pressing the mixture into tablets by a tablet press to obtain the calcium supplement giant salamander bone calcium tablets.

Example 2

The giant salamander bone calcium tablet for supplementing calcium comprises the following raw materials in parts by weight: 40 parts of giant salamander bone meal, 50 parts of chondroitin sulfate, 5 parts of vitamin and 1 part of magnesium stearate;

the giant salamander bone meal is prepared from giant salamander bones with cartilages, the chondroitin sulfate is prepared from the giant salamander cartilages, and the mass ratio of the giant salamander cartilages to the giant salamander bones is 9: 1.

Example 2 preparation method of calcium-supplementing giant salamander bone calcium tablets is the same as example 1.

Example 3

The giant salamander bone calcium tablet for supplementing calcium comprises the following raw materials in parts by weight: 80 parts of giant salamander bone meal, 15 parts of chondroitin sulfate, 10 parts of vitamin and 0.1 part of magnesium stearate;

the giant salamander bone meal is prepared from giant salamander bones with cartilages, the chondroitin sulfate is prepared from the giant salamander cartilages, and the mass ratio of the giant salamander cartilages to the giant salamander bones is 1: 9.

Example 3 preparation method of calcium-supplementing giant salamander bone calcium tablets is the same as example 1.

Example 4

The giant salamander bone calcium tablet for supplementing calcium comprises the following raw materials in parts by weight: 70 parts of giant salamander bone meal, 22 parts of chondroitin sulfate, 8.75 parts of vitamin and 0.35 part of magnesium stearate.

The giant salamander bone meal is prepared from giant salamander bones with cartilages, the chondroitin sulfate is prepared from the giant salamander cartilages, and the mass ratio of the giant salamander cartilages to the giant salamander bones is 5: 12.

Example 4 preparation method of calcium-supplementing giant salamander bone calcium tablets is the same as example 1.

Example 5

The giant salamander bone calcium tablets for supplementing calcium comprise the following raw materials, by weight, 50 parts of giant salamander bone powder, 37 parts of chondroitin sulfate, 6.25 parts of vitamin and 0.75 part of magnesium stearate;

the giant salamander bone meal is prepared from giant salamander bones with cartilages, the chondroitin sulfate is prepared from the giant salamander cartilages, and the mass ratio of the giant salamander cartilages to the giant salamander bones is 6: 5.

Example 5 preparation method of calcium-supplemented giant salamander bone is the same as example 1.

In conclusion, the giant salamander bone calcium tablet for supplementing calcium is prepared from giant salamander bone meal, chondroitin sulfate, vitamins and magnesium stearate as raw materials, has the advantage of high calcium content, and is suitable for daily calcium supplementation of various crowds. In the enzymolysis process of the giant salamander, the cartilage of the giant salamander cannot be enzymolyzed together with the giant salamander meat, and the complete cartilage can still be remained after enzymolysis.