阅读说明：本技术 一种兽用止痛重楼注射液及其制备工艺 (Analgesic rhizoma paridis injection for livestock and preparation process thereof ) 是由 王宝贵 刘文莉 于 2021-07-21 设计创作，主要内容包括：本发明公开了一种兽用止痛重楼注射液,10000毫升该重楼注射液中含有以下成分及重量份：重楼约3千克,吐温-80 80-100毫升,苯甲醇80-100毫升,余量为注射用水。该重楼注射液的制备工艺包括以下步骤：将重楼粉碎后；再加入一定量的注射用水进行煎煮,过滤后进行浓缩,用乙醇多次调整醇含量并浓缩抽滤回收乙醇,再用注射用水进行稀释冷藏,再用活性炭进行煮沸吸附,过滤回收活性炭,用氢氧化钠调节PH值,加吐温-80和苯甲醇后再次稀释定容,最后灭菌罐装。本发明不仅能够有效缓解和减轻动物的疼痛,且减少各种并发症的产生,具有良好的疗效和广阔的市场前景。(The invention discloses an analgesic paris polyphylla injection for livestock, 10000 milliliters of paris polyphylla injection comprises the following components in parts by weight: rhizoma paridis 3 kg, Tween-8080-. The preparation process of the paris polyphylla injection comprises the following steps: pulverizing rhizoma paridis; adding a certain amount of water for injection, decocting, filtering, concentrating, adjusting alcohol content with ethanol for multiple times, concentrating, vacuum filtering, recovering ethanol, diluting with water for injection, refrigerating, boiling with active carbon, filtering, recovering active carbon, adjusting pH with sodium hydroxide, adding tween-80 and benzyl alcohol, diluting again, diluting to desired volume, sterilizing, and canning. The traditional Chinese medicine composition can effectively relieve and relieve pain of animals, reduces various complications, and has good curative effect and wide market prospect.)

1. An analgesic paris polyphylla injection for animals is characterized in that: 10000ml of the paris polyphylla injection contains the following components in parts by weight: rhizoma paridis 3 kg, Tween-8080-.

2. The analgesic paris polyphylla injection for veterinary use as claimed in claim 1, wherein: 10000ml of the paris polyphylla injection contains the following components in parts by weight: rhizoma paridis 3 kg, tween-80100 ml, benzyl alcohol 80 ml, and water for injection in balance.

3. The analgesic paris polyphylla injection for veterinary use as claimed in claim 1, wherein: 10000ml of the paris polyphylla injection contains the following components in parts by weight: rhizoma paridis 3 kg, tween-8080 ml, benzyl alcohol 100 ml, and water for injection in balance.

4. The analgesic paris polyphylla injection for veterinary use as claimed in claim 1, wherein: 10000ml of the paris polyphylla injection contains the following components in parts by weight: rhizoma paridis 3 kg, tween-8090 ml, benzyl alcohol 90 ml, and the balance of water for injection.

5. A process for preparing the analgesic rhizoma paridis injection for animals according to claim 1, which comprises: the method comprises the following steps: pulverizing rhizoma paridis as main material, decocting, precipitating, filtering, adjusting ethanol content, adding water for injection for dilution, stirring, refrigerating, filtering, adsorbing with active carbon, refrigerating, filtering, adjusting pH with NaOH, hot pressing, bottling, and sterilizing to obtain the final product.

6. The preparation process of the analgesic rhizoma paridis injection for animals according to claim 5, wherein the injection comprises the following steps: the method comprises the following specific steps:

1) cleaning rhizoma paridis, sun drying, and pulverizing; removing impurities from the medicines, weighing, adding 25000 ml of injection water and 30000 ml of injection water, soaking for 40-60 minutes, and then decocting; filtering the obtained medicinal liquid with four layers of gauze; the four filtrates are combined and concentrated to 3100 ml 2900-;

2) adding ethanol into the concentrated solution obtained in the step 1) to adjust the ethanol content to be more than 60%, fully stirring, standing for 24 hours, performing suction filtration on two layers of filter paper, recovering ethanol, and concentrating to 3100 ml 2900-;

3) adding ethanol into the concentrated solution obtained in the step 2) to adjust the ethanol content to be more than 75%, standing for about 40 hours, performing suction filtration by using three layers of filter paper, recovering the ethanol, and concentrating to 3100 ml of 2900-;

4) adding water for injection to 8500 ml into the concentrated solution obtained in the step 3), fully stirring, refrigerating for 48 hours below 0 ℃, and performing suction filtration by three layers of filter paper or drip filtration by one layer of filter paper;

5) adding 50 g of activated carbon into the filtrate obtained in the step 4), boiling for 5 minutes, fully stirring for cooling, refrigerating for 24 hours, carrying out suction filtration or drip filtration by using three layers of filter paper, and simultaneously recovering the activated carbon;

6) adding 20% NaOH solution into the filtrate obtained in the step 5) to adjust the pH to 7-7.5, adding 100 ml of Tween 8080-; placing into 100 ml penicillin bottles, and sterilizing at 110 ℃ for 30 minutes in a hot pressing manner for later use;

7) directly canning with 3# filter ball during filling, and sealing by fusing; sterilizing at 100 deg.C for 30 min, lamp-inspecting, heat-testing, printing, and packaging.

7. The preparation process of the analgesic rhizoma paridis injection for animals according to claim 6, wherein the injection comprises the following steps: decocting the rhizoma paridis twice in the step 1), wherein each time lasts for about two hours, filtering the obtained liquid medicine by using four layers of gauze, and keeping the filtrate for later use.

Technical Field

The invention relates to a Chinese herbal medicine preparation, in particular to a preparation process of an analgesic paris polyphylla injection for livestock.

Background

The paris polyphylla is dried rhizome of Yunnan paris polyphylla or paris polyphylla of liliaceae, the paris polyphylla is a unique and rare medicinal material in China and is a perennial herb, the paris polyphylla is collected in Shennong herbal tea with the name of paris polyphylla, at present, the paris polyphylla is mainly cultivated manually, leaves 6-10 are grown in a rotation mode, the length of a leaf stalk is 5-20mm, the leaves are thick paper, the leaves are needle-shaped, oval long and inverted egg-shaped, green and the length is 3.5-6 cm; the perianth sheet is linear and slightly needle-shaped and yellow, the length of the perianth sheet is about 1/2 of sepals to be approximately equal, and the width of the middle part is 2-6 mm; stamen 8-10mm, anther 1-1.5cm long, filament shorter than anther, and drug separation 1-2 mm. Rhizoma paridis contains various steroidal saponins, namely ditridecyloside of diosgenin and pennogenin, and beta-ecdysone, daucosterol and the like, and is a main raw material of various antibacterial, anti-inflammatory and analgesic medicines.

Rhizoma paridis has the effects of clearing heat and removing toxicity, and relieving swelling and pain, is a common medicine for treating chaff carbuncle snake bite, can treat sore throat and joint pain, but at present, rhizoma paridis is rarely prepared into injection for relieving pain of animals.

Disclosure of Invention

In order to solve the defects and shortcomings in the prior art, the invention provides the preparation process of the veterinary analgesic paris polyphylla injection, which has the functions of diminishing inflammation and sterilizing, has good effect on animal pain relief, can effectively relieve and relieve animal pain, does not generate hemolytic reaction, has no vascular irritation or systemic sensitization, reduces various complications, and has good curative effect and wide market prospect.

The technical scheme of the invention is as follows: 10000ml of paris polyphylla injection for relieving pain for animals comprises the following components in parts by weight: rhizoma paridis 3 kg, Tween-8080-.

The traditional Chinese medicine composition has the functions of diminishing inflammation and sterilizing, has a good effect of relieving pain of animals, can effectively relieve and relieve the pain of the animals, does not generate hemolytic reaction, has no vascular irritation or systemic sensitization, reduces the generation of various complications, and has good curative effect and wide market prospect.

Preferably, 10000ml of the rhizoma paridis injection contains the following components in parts by weight: rhizoma paridis 3 kg, tween-80100 ml, benzyl alcohol 80 ml, and water for injection in balance.

Preferably, 10000ml of the rhizoma paridis injection contains the following components in parts by weight: rhizoma paridis 3 kg, tween-8080 ml, benzyl alcohol 100 ml, and water for injection in balance.

Preferably, 10000ml of the rhizoma paridis injection contains the following components in parts by weight: rhizoma paridis 3 kg, tween-8090 ml, benzyl alcohol 90 ml, and the balance of water for injection.

A preparation process of an analgesic rhizoma paridis injection for animals comprises the following steps: pulverizing rhizoma paridis as main material, decocting, precipitating, filtering, adjusting ethanol content, adding water for injection for dilution, stirring, refrigerating, filtering, adsorbing with active carbon, refrigerating, filtering, adjusting pH with NaOH, hot pressing, bottling, and sterilizing to obtain the final product.

Preferably, the preparation process of the veterinary analgesic paris polyphylla injection comprises the following specific steps:

1) cleaning rhizoma paridis, sun drying, and pulverizing; removing impurities from the medicines, weighing, adding 25000 ml of injection water and 30000 ml of injection water, soaking for 40-60 minutes, and then decocting; filtering the obtained medicinal liquid with four layers of gauze; the filtrate was concentrated to 2900-;

2) adding ethanol into the concentrated solution obtained in the step 1) to adjust the ethanol content to be more than 60%, fully stirring, standing for 24 hours, performing suction filtration on two layers of filter paper, recovering ethanol, and concentrating to 3100 ml 2900-;

3) adding ethanol into the concentrated solution obtained in the step 2) to adjust the ethanol content to be more than 75%, standing for about 40 hours, performing suction filtration by using three layers of filter paper, recovering the ethanol, and concentrating to 3100 ml of 2900-;

4) adding water for injection to 8500 ml into the concentrated solution obtained in the step 3), fully stirring, refrigerating for 48 hours below 0 ℃, and performing suction filtration by three layers of filter paper or drip filtration by one layer of filter paper;

5) adding 50 g of activated carbon into the filtrate obtained in the step 4), boiling for 5 minutes, fully stirring for cooling, refrigerating for 24 hours, carrying out suction filtration or drip filtration by using three layers of filter paper, and simultaneously recovering the activated carbon;

6) adding 20% NaOH solution into the filtrate obtained in the step 5) to adjust the pH to 7-7.5, adding Tween-8080-100 ml, benzyl alcohol 80-100 ml and water for injection to 10000ml, stirring and filtering; placing into 100 ml penicillin bottles, and sterilizing at 110 ℃ for 30 minutes in a hot pressing manner for later use;

7) directly canning with 3# filter ball during filling, and sealing by fusing; sterilizing at 100 deg.C for 30 min, lamp-inspecting, heat-testing, printing, and packaging.

Preferably, the rhizoma paridis residue obtained in step 1) is decocted twice, each time lasts for about two hours, the obtained liquid medicine is filtered by four layers of gauze, and the filtrate is reserved.

The traditional Chinese medicine composition has the functions of diminishing inflammation and sterilizing, has a good effect of relieving pain of animals, can effectively relieve and relieve the pain of the animals, does not generate hemolytic reaction, has no vascular irritation or systemic sensitization, reduces the generation of various complications, and has good curative effect and wide market prospect.

Detailed Description

The present invention is further described in detail with reference to the following examples, but the scope of the present invention is not limited thereto.

Example 1

A preparation process of an analgesic rhizoma paridis injection for animals comprises the following steps:

(a) cleaning rhizoma paridis, sun drying, and pulverizing; removing impurities from the medicines, weighing, adding 25000 and 30000 milliliters of water, soaking for 40-60 minutes, and then decocting. Decocting rhizoma paridis (residue) twice, each for about two hours, and filtering the obtained liquid medicine with four layers of gauze. The filtrate was concentrated to about 3000 ml.

(b) Adding ethanol into the concentrated solution to adjust the ethanol content to be more than 60%, fully stirring, standing for 24 hours, performing suction filtration on two layers of filter paper, recovering ethanol, and concentrating to about 3000 ml.

(c) Adding ethanol into the concentrated solution to adjust the alcohol content to be more than 75%, standing for about 40 hours, carrying out suction filtration by using three layers of filter paper, recovering ethanol, and concentrating to about 3000 ml.

(d) Adding water for injection into the concentrated solution to 8500 ml, stirring thoroughly, refrigerating at below 0 deg.C for 48 hr, and vacuum filtering with three layers of filter paper or filtering with one layer of filter paper.

(e) Adding 50 g of activated carbon into the filtrate, boiling for 5 minutes, fully stirring for cooling, refrigerating for 24 hours, carrying out suction filtration or trickling filtration by using three layers of filter paper, and simultaneously recovering the activated carbon.

(f) Adjusting pH of the filtrate to 7-7.5 with 20% NaOH solution, adding tween-8080 and 100 ml of benzyl alcohol, adding water for injection to 10000ml, stirring, and filtering. Packaging into 100 ml penicillin bottles, and autoclaving at 110 ℃ for 30 minutes for later use.

(g) And (3) directly canning by connecting a 3# filter ball during canning, and sealing by fusing. Sterilizing at 100 deg.C for 30 min, lamp-inspecting, heat-testing, printing, and packaging.

The analgesic paris polyphylla injection is prepared according to the preparation process, 10000mL of analgesic paris polyphylla injection is obtained (the paris polyphylla injection comprises the following components in parts by weight of 3 kg of paris polyphylla, 90 mL of tween-8090, 90 mL of benzyl alcohol and the balance of water for injection), pigs of the same variety are fed according to the experiments of 8 percent and 15 percent of dose and blank control components, and the results after 1 month are shown in the following table:

	prevalence rate	Rate of weight gain
			Blank control group	63%	73%
8% addition group	51%	85%
			15% addition group	19%	116%

As can be seen from the above table, the weight gain of the pigs in the 15% addition group is significantly higher than that of the blank control group and that of the 8% addition group; and the prevalence rate of the 15% addition group pigs is far lower than that of the blank control group and the 8% addition group.

The conventional techniques in the above examples are known to those skilled in the art, and are not described in detail herein, for example, conventional techniques such as filtration, concentration and impurity removal.

It is to be understood that the above description is not intended to limit the present invention, and the present invention is not limited to the above examples, and those skilled in the art may make modifications, alterations, additions or substitutions within the spirit and scope of the present invention.