阅读说明：本技术 一种柴胡炮制品及其炮制方法 (Radix bupleuri processed product and processing method thereof ) 是由 郝婷 赵桂琴 李洪波 毛晓霞 李松涛 傅万峪 于 2021-11-05 设计创作，主要内容包括：本发明公开了一种柴胡炮制品及其炮制方法,该方法包括以下步骤：将柴胡生品饮片用小苏打水溶液浸润；将浸润后的柴胡生品饮片用文火炒干,放凉后,得到柴胡炮制品。该炮制方法简单、易操作,制得柴胡炮制品中柴胡总皂苷含量比柴胡生品饮片高约2倍,柴胡皂苷a、d含量比柴胡生品饮片高2倍以上,水煎煮时,本发明制备的柴胡炮制品浸出物含量比柴胡生饮片高。(The invention discloses a radix bupleuri processed product and a processing method thereof, wherein the method comprises the following steps: soaking radix bupleuri crude decoction pieces in sodium bicarbonate water solution; and (4) frying the soaked radix bupleuri crude decoction pieces with slow fire, and cooling to obtain the radix bupleuri processed product. The processing method is simple and easy to operate, the total saikosaponin content of the prepared bupleurum root product is about 2 times higher than that of the raw bupleurum root decoction pieces, the content of saikosaponin a and saikosaponin d is more than 2 times higher than that of the raw bupleurum root decoction pieces, and when the prepared bupleurum root product is decocted by water, the extract content of the prepared bupleurum root product is higher than that of the raw bupleurum root decoction pieces.)

1. The processing method of the radix bupleuri processed product is characterized by comprising the following steps:

(1) soaking radix bupleuri crude decoction pieces in sodium bicarbonate water solution;

(2) parching the soaked radix bupleuri crude decoction pieces with slow fire, and cooling to obtain radix bupleuri processed product.

2. The processing method of the radix bupleuri processed product according to claim 1, wherein the radix bupleuri raw material decoction pieces in the step (1) are prepared by removing impurities and residual stems from radix bupleuri raw material, cleaning, moistening, slicing and drying to obtain the radix bupleuri raw material decoction pieces_。

3. The processing method of the processed bupleurum root product as claimed in claim 2, wherein the drying temperature is 58-63 ℃.

4. The processing method of the radix bupleuri processed product according to claim 1, wherein the mass ratio of the radix bupleuri raw material decoction pieces to the baking soda in the step (1) is 100: 1-6, and the concentration of the sodium bicarbonate aqueous solution is 60-100 g/L.

5. The processing method of processed radix bupleuri products as claimed in claim 1, wherein the soaking time in step (1) is 0.5-3 h.

6. The processing method of processed bupleuri radix as claimed in claim 1, wherein the temperature of the fire in step (2) is 100-200 ℃.

7. The processing method of the radix bupleuri processed product according to claim 2, wherein the parching time with slow fire in the step (2) is 5-10 min.

8. The processed radix bupleuri product prepared by the processing method of the processed radix bupleuri product according to any one of claims 1 to 7.

Technical Field

The invention relates to the technical field of processing of traditional Chinese medicine decoction pieces, in particular to a radix bupleuri processed product and a processing method thereof.

Background

The Chinese medicinal material bupleuri radix is derived from dried root of Bupleurum chinense DC or Bupleurum angustifolium Willd belonging to Umbelliferae, and has been used as traditional Chinese medicine for thousands of years. The research shows that: the bupleuri radix contains saponin, volatile oil, polysaccharide, flavone, etc., and has antiinflammatory, antibacterial, and antiviral effects. Saikosaponin (saikoaponins, SS) is the main chemical and bioactive components of bupleurum, and the chinese pharmacopoeia (first part of 2020 edition) uses the total amount of Saikosaponin a (saikoaponin a, SSa) and Saikosaponin d (saikoaponin d, SSd) as the quality control index of bupleurum medicinal material. Both SSa and SSd belong to the class of native saponins. Clinically, the bupleurum is mainly decocted into a decoction, so that the content of SSa and SSd in the decoction can be influenced, and further the drug effect is influenced.

Disclosure of Invention

In order to solve the technical problems, the invention aims to provide a radix bupleuri processed product and a processing method thereof, so as to solve the problems of low saikosaponin content and low dissolution rate in the conventional radix bupleuri processed product.

The technical scheme for solving the technical problems is as follows: provides a processing method of a radix bupleuri processed product, which comprises the following steps:

(1) soaking radix bupleuri crude decoction pieces in sodium bicarbonate water solution;

(2) parching the soaked radix bupleuri crude decoction pieces with slow fire, and cooling to obtain radix bupleuri processed product.

The invention has the beneficial effects that: saikosaponin (saikoaponins, SS) is the main chemical and bioactive components of bupleurum, and the chinese pharmacopoeia (first part of 2020 edition) uses the total amount of Saikosaponin a (saikoaponin a, SSa) and Saikosaponin d (saikoaponin d, SSd) as the quality control index of bupleurum medicinal material. SSa and SSd both belong to primary saponins, epoxy ether bonds in the structures of the primary saponins are unstable, and are easy to crack to form secondary saponins, the bupleurum is processed by an alkaline method, and the cracking of the epoxy ether bonds can be inhibited in an alkaline environment, so the content of the saikosaponin in the bupleurum can be improved, and meanwhile, certain combined type saikosaponin can be directly or indirectly converted into free type saikosaponin in the roasting processing process, so the content of the saikosaponin in the bupleurum is further improved, and the saikosaponin is easier to dissolve out under the alkaline condition, so the dissolution rate of the saikosaponin in the bupleurum can be improved.

On the basis of the technical scheme, the invention can be further improved as follows:

further, the raw radix bupleuri decoction pieces in the step (1) are prepared by removing impurities and residual stems from raw radix bupleuri medicinal materials, cleaning, moistening, slicing into thick pieces and drying.

Further, the drying temperature was 58 to 63 ℃.

Further, in the step (1), the mass ratio of the radix bupleuri crude decoction pieces to the baking soda is 100: 1-6, and the concentration of the sodium bicarbonate aqueous solution is 60-100 g/L.

Further, the soaking time in the step (1) is 0.5-3 h.

Further, the temperature of the fire in the step (2) is 100-200 ℃.

Further, the time for frying dry in the step (2) by slow fire is 5-10 min.

The invention has the following beneficial effects: the prepared bupleurum root processed product has the content of the total saikosaponin which is about 2 times higher than that of the raw bupleurum root decoction pieces, and the content of the saikosaponin a and the saikosaponin d which is more than 2 times higher than that of the raw bupleurum root decoction pieces; as the bupleurum root is mainly used as a decoction in clinic, the dissolution rate of saikosaponin a and saikosaponin d in the prepared bupleurum root product is about 2 times higher than that of bupleurum root crude decoction pieces when the bupleurum root is decocted in water.

Detailed Description

The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

Example 1:

a processing method of a radix bupleuri processed product comprises the following steps:

(1) removing impurities and residual stems of radix bupleuri raw medicinal materials, cleaning with clear water, moistening with clear water until the medicinal materials are thoroughly permeated, cutting into 8mm thick pieces, and drying in a 60 ℃ oven to obtain radix bupleuri raw medicinal slices;

(2)1g baking soda (NaHCO)₃) Adding 10mL of distilled water, and dissolving by ultrasonic to prepare a sodium bicarbonate water solution;

(3) soaking 50g of radix bupleuri crude decoction pieces in 20mL of sodium bicarbonate aqueous solution containing 2g of sodium bicarbonate for 3 h;

(4) parching the soaked radix bupleuri crude decoction pieces with slow fire at 100 deg.C for 10min, taking out, cooling, and making into radix bupleuri processed product.

Example 2:

a processing method of a radix bupleuri processed product comprises the following steps:

(1) removing impurities and residual stems of radix bupleuri raw medicinal materials, cleaning with clear water, moistening with clear water until the medicinal materials are thoroughly permeated, cutting into 8mm thick pieces, and oven drying at 58 deg.C to obtain radix bupleuri crude decoction pieces;

(2)1g baking soda (NaHCO)₃) Adding 10mL of distilled water, and dissolving by ultrasonic to prepare a sodium bicarbonate water solution;

(3) soaking 50g of radix bupleuri crude decoction pieces in 30mL of sodium bicarbonate aqueous solution containing 3g of sodium bicarbonate for 2 h;

(4) parching the soaked radix bupleuri crude decoction pieces with slow fire at 180 deg.C for 6min, taking out, cooling, and making into radix bupleuri processed product.

Example 3:

a processing method of a radix bupleuri processed product comprises the following steps:

(1) removing impurities and residual stems of radix bupleuri raw medicinal materials, cleaning with clear water, moistening with clear water until the medicinal materials are thoroughly permeated, cutting into 8mm thick pieces, and oven drying at 63 deg.C to obtain radix bupleuri crude decoction pieces;

(2)1g baking soda (NaHCO)₃) Adding 15mL of distilled water, and dissolving by ultrasonic to prepare a sodium bicarbonate water solution;

(3) soaking 50g of radix bupleuri crude decoction pieces in 30mL of sodium bicarbonate aqueous solution containing 1g of sodium bicarbonate for 2 h;

(4) parching the soaked radix bupleuri crude decoction pieces with slow fire at 120 deg.C for 8min, taking out, cooling, and making into radix bupleuri processed product.

Comparative example 1

A processing method of radix bupleuri decoction pieces comprises the following steps: removing impurities and residual stems of radix bupleuri raw medicinal materials, cleaning with clear water, moistening with clear water until the medicinal materials are thoroughly permeated, cutting into 8mm thick pieces, and drying in a 60 ℃ oven to obtain radix bupleuri raw medicinal slices.

Result detection

Contents of one, total saponins, SSa and SSd

The contents of total saponins in bupleuri radix prepared in examples 1-3 and comparative example 1 were respectively detected by UV spectrophotometry.

The content of SSa and SSd in the bupleurum prepared in the examples 1-3 and the comparative example 1 is respectively detected by adopting a high performance liquid chromatography and referring to a method of content determination under the item of bupleurum in the Chinese pharmacopoeia (the first part of 2020 edition), and the specific detection method comprises the following steps: respectively taking the processed radix bupleuri products prepared in examples 1-3 and the raw radix bupleuri decoction pieces prepared in comparative example 1, and crushing to obtain radix bupleuri powder (sieving by a sieve IV); chromatographic conditions and system applicability test: using octadecylsilane chemically bonded silica as a filler, acetonitrile as a mobile phase A, water as a mobile phase B, and performing gradient elution under the following conditions: acetonitrile (A) -water (B), gradient elution (0-30min, 35% A, 30-50min, 35% -90% A, 50-60min, 90%); the detection wavelength is 210nm, and the number of theoretical plates is not less than 10000 calculated according to the peak of saikosaponin a; preparation of control solutions: taking proper amount of saikosaponin a reference substance and saikosaponin d reference substance, precisely weighing, adding methanol to obtain solution containing saikosaponin a0.4mg and saikosaponin d0.5mg per 1mL, and shaking up to obtain; preparation of a test solution: taking about 0.5g of powder of a product to be detected (passing through a No. four sieve), precisely weighing, placing the powder in a conical flask with a plug, adding 25mL of methanol solution containing 5% concentrated ammonia test solution, sealing the plug, carrying out ultrasonic treatment (power 200W and frequency 40kHz) at 30 ℃ for 30 minutes, filtering, washing a container and medicine residues by 20mL of methanol for 2 times, combining the washing solution and the filtrate, recovering the solvent to dryness, dissolving the residues in methanol, transferring the solution to a 5mL measuring flask, adding methanol to scale, shaking up, filtering, and taking a subsequent filtrate to obtain the product; the determination method comprises the following steps: precisely sucking 20 μ L of the reference solution and 10-20 μ L of the sample solution, respectively, injecting into a liquid chromatograph, and measuring; the powder to be detected contains saikosaponin a and saikosaponin d in percentage by mass based on the dry product.

The above detection results are shown in table 1, and it can be seen from table 1 that the total saikosaponin content of the processed product of bupleurum root prepared by the present invention is about 2 times higher than that of the raw bupleurum root, and the content of saikosaponin a and saikosaponin d is more than 2 times higher than that of the raw bupleurum root.

TABLE 1 Total saponins, SSa and SSd content (% by mass) in Bupleurum chinense

Content (wt.)	Total saponins	SSa	SSd
				Example 1	4.803	1.270	1.477
Example 2	6.211	1.245	1.528
				Example 3	3.921	0.76	0.650
Comparative example 1	2.357	0.373	0.484

Second, the dissolution rate of saikosaponin obtained by decocting bupleurum root with water

Clinically, the bupleurum roots prepared in the example 1 and the comparative example 1 are respectively decocted by distilled water, the contents of the total saponins, SSa and SSd in the extracting solution are detected by an ultraviolet spectrophotometry and a high performance liquid chromatography, the dissolution rates (the percentages of the dissolved substances to the mass of the processed product) of the total saponins, SSa and SSd are obtained, the detection results are shown in table 2, and the dissolution rates of the saikosaponin a and the saikosaponin d in the processed product of the bupleurum roots prepared by the invention are 2 times higher than that of raw bupleurum decoction pieces when the bupleurum roots are decocted by water.

TABLE 2 dissolution rate (% by mass) of total saponins, SSa and SSd in Bupleurum chinense

Content (wt.)	Total saponins	SSa	SSd
				Example 1	0.875	0.531	0.335
Comparative example 1	0.764	0.260	0.154

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.