阅读说明：本技术 一种益生菌配方奶粉及其制备方法 (Probiotic formula milk powder and preparation method thereof ) 是由 孟锦华 刘慧� 于 2021-08-13 设计创作，主要内容包括：本发明公开了一种益生菌配方奶粉及其制备方法,益生菌奶粉由鲜羊乳、脱盐乳清粉、浓缩乳清蛋白、水苏糖、维生素C、直投式发酵剂和益生菌粉组成其配比为：鲜羊乳5500-6500份脱盐乳清粉110-245份、浓缩乳清蛋白30-50份、水苏糖20-40份、维生素C0.3-1份、直投式发酵剂0.5-1份、益生菌粉0.006-0.2份。本发明通过鲜羊乳、脱盐乳清粉、浓缩乳清蛋白、水苏糖、维生素C、直投式发酵剂和益生菌粉的精确配比,提高了奶粉的营养程度,通过鲜羊乳对婴幼儿、孕妇、老人和病人均具有很好的营养与保健功效,通过添加益生菌粉,有利于促进肠道吸收和蠕动,通过添加维生素C,可提高人自身的杀菌性能,从而充分的提高了奶粉的营养价值。(The invention discloses a probiotic formula milk powder and a preparation method thereof, wherein the probiotic milk powder comprises fresh goat milk, desalted whey powder, concentrated whey protein, stachyose, vitamin C, a direct vat set starter and probiotic powder, and the ratio is as follows: 5500 portions of fresh goat milk and 6500 portions of 110 portions of desalted whey powder and 245 portions of fresh goat milk, 30 to 50 portions of concentrated whey protein, 20 to 40 portions of stachyose, 0.3 to 1 portion of vitamin C, 0.5 to 1 portion of direct vat set starter and 0.006 to 0.2 portion of probiotic powder. According to the invention, the nutrition degree of the milk powder is improved by the accurate proportion of the fresh goat milk, the desalted whey powder, the concentrated whey protein, the stachyose, the vitamin C, the direct vat set starter and the probiotic powder, the milk powder has good nutrition and health care effects on infants, pregnant women, old people and patients, the intestinal absorption and peristalsis are facilitated by adding the probiotic powder, the sterilization performance of the human body can be improved by adding the vitamin C, and thus the nutrition value of the milk powder is fully improved.)

1. The probiotic formula milk powder is characterized in that: the probiotic milk powder consists of fresh goat milk, desalted whey powder, concentrated whey protein, stachyose, vitamin C, a direct vat set starter and probiotic powder, and the ratio is as follows: 5500 portions of fresh goat milk and 6500 portions, 110 portions of desalted whey powder and 245 portions, 30 to 50 portions of concentrated whey protein, 20 to 40 portions of stachyose, 0.3 to 1 portion of vitamin C, 0.5 to 1 portion of direct vat set starter and 0.006 to 0.2 portion of probiotic powder.

2. The probiotic formula milk powder according to claim 1, characterized in that: the probiotic milk powder consists of fresh goat milk, desalted whey powder, concentrated whey protein, stachyose, vitamin C, a direct vat set starter and probiotic powder, and the ratio is as follows: 5500 parts of fresh goat milk, 110 parts of desalted whey powder, 30 parts of concentrated whey protein, 20 parts of stachyose, 0.3 part of vitamin C, 0.5 part of direct vat set starter and 0.006 part of probiotic powder.

3. The probiotic formula milk powder according to claim 1, characterized in that: the probiotic milk powder consists of fresh goat milk, desalted whey powder, concentrated whey protein, stachyose, vitamin C, a direct vat set starter and probiotic powder, and the ratio is as follows: 6500 parts of fresh goat milk, 245 parts of desalted whey powder, 50 parts of concentrated whey protein, 40 parts of stachyose, 1 parts of vitamin C, 1 part of direct vat set starter and 0.2 part of probiotic powder.

4. A probiotic formula milk powder and a method for preparing the same according to any one of claims 1 to 3, characterized in that: the preparation method comprises the following steps:

A. the method comprises the following steps of (1) purchasing fresh milk, desalted whey powder, concentrated whey protein, stachyose and vitamin C in proportion:

B. preparing a direct vat set starter:

b1, mixing the lactobacillus bulgaricus powder and the streptococcus thermophilus powder according to the mass ratio of 2:1, so that the ratio of the viable count of the lactobacillus bulgaricus powder to the streptococcus thermophilus powder is 1:1, and obtaining the direct vat set starter;

C. preparing probiotic powder;

c1, adding 1.2% of lactose, 0.5% of peptone, 0.25% of xylo-oligosaccharide, 0.20% of sodium acetate and 0.22% of ammonium citrate tribasic into 10% of allium macrostemon extract according to the mass-volume ratio to prepare a culture medium;

c2, sterilizing and cooling a culture medium, respectively inoculating activated probiotics, culturing at a constant temperature of 37 ℃ for 24h, freezing and centrifuging to obtain probiotic bacterial paste, adding a composite freeze-drying protective agent which is 1.5 times the weight of the probiotic bacterial paste, performing cold treatment at 10 ℃ for 1.5h, and performing freeze-drying by using a vacuum freeze-drying machine for 24h to obtain probiotic powder;

D. sterilizing and filtering the fresh goat milk;

d1, performing primary filtration sterilization on the fresh milk through a 200-mesh sterilization filter screen;

d2, performing secondary filtration sterilization on the fresh milk through a 400-mesh sterilization filter screen;

E. d, uniformly stirring the filtered and sterilized fresh goat milk obtained in the step D, desalted whey powder, concentrated whey protein, stachyose and vitamin C to obtain a mixture;

F. e, performing high-temperature instantaneous sterilization on the mixture obtained in the step E;

G. f, cooling the mixture subjected to high-temperature sterilization obtained in the step F;

H. g, carrying out reduced pressure concentration on the mixture obtained in the step G after the temperature is reduced to obtain a concentrated milk material;

I. d, spray drying the concentrated milk material obtained in the step H to obtain prebiotics milk powder;

J. adding the direct vat set starter and the probiotic powder, and uniformly stirring again to obtain probiotic milk powder;

K. packaging;

k1, canning and sealing the probiotic milk powder obtained in the step J by a packaging machine.

5. The probiotic formula milk powder and the preparation method thereof according to claim 4, wherein the probiotic formula milk powder comprises the following components: and D, filtering and sterilizing the fresh milk in an aseptic environment, wherein the filtering times are 2-3.

6. The probiotic formula milk powder and the preparation method thereof according to claim 4, wherein the probiotic formula milk powder comprises the following components: and E, uniformly stirring the fresh goat milk, the desalted whey powder, the concentrated whey protein, the stachyose and the vitamin C by using a stirrer at the rotating speed of 220 r/min.

7. The probiotic formula milk powder and the preparation method thereof according to claim 4, wherein the probiotic formula milk powder comprises the following components: and F, instantly sterilizing by a high-temperature sterilizer at 120-150 ℃ for 3-9 s.

8. The probiotic formula milk powder and the preparation method thereof according to claim 4, wherein the probiotic formula milk powder comprises the following components: and G, naturally cooling the mixture subjected to high-temperature sterilization for 4 hours in an aseptic environment.

9. The probiotic formula milk powder and the preparation method thereof according to claim 4, wherein the probiotic formula milk powder comprises the following components: and step I, spray drying the concentrated feed material by a spray dryer, wherein the air inlet temperature is 150 ℃ and the air outlet temperature is 60 ℃.

Technical Field

The invention relates to the technical field of dairy production, in particular to probiotic formula milk powder and a preparation method thereof.

Background

The milk powder is powder prepared by taking milk of cows, sheep or other animals as a raw material through processes of disinfection, degreasing, dehydration, drying and the like, is a reprocessed product rather than an original food, is suitable for storage and convenient to carry, is added with a proper amount of nutrient components such as vitamins, mineral substances and the like, and can be used for brewing and drinking, food processing, canned beverages and the like; the probiotics refer to living microorganisms beneficial to human bodies, when enough dosage is given, the probiotics are beneficial to the health of hosts, along with social development and progress, the types of milk powder are more and more, in the process of processing the milk powder, other nutritional ingredients are artificially added to enable the milk powder to be more nutritional, along with the continuous improvement of the living standard of people, the requirement of people on diet is also continuously improved, people not only stay on the level of satiety, but also pay more attention to the nutritional function of diet and the safety problem of food, and from the current market, the milk powder has good quality, generally high value and less and really plays a role in health care, so that the probiotics formula milk powder and the preparation method thereof are urgently needed.

Disclosure of Invention

The invention aims to provide probiotic formula milk powder and a preparation method thereof, which have the advantages of nutrition and gastrointestinal absorption promotion, and solve the problems that as the living standard of people is continuously improved, the requirements of people on diet are also continuously improved, people not only stay on the aspect of full eating, but also pay more attention to the nutritional function of diet and the safety of food, and the problems that the quality of milk powder is good and uneven, the value is generally high, and the health-care effect is really played are few and few in the current market.

In order to achieve the purpose, the invention provides the following technical scheme: a probiotic formula milk powder and a preparation method thereof, the probiotic milk powder is composed of fresh goat milk, desalted whey powder, concentrated whey protein, stachyose, vitamin C, a direct vat set starter and probiotic powder, and the proportion is as follows: 5500 portions of fresh goat milk and 6500 portions, 110 portions of desalted whey powder and 245 portions, 30 to 50 portions of concentrated whey protein, 20 to 40 portions of stachyose, 0.3 to 1 portion of vitamin C, 0.5 to 1 portion of direct vat set starter and 0.006 to 0.2 portion of probiotic powder.

Preferably, the probiotic milk powder consists of fresh goat milk, desalted whey powder, concentrated whey protein, stachyose, vitamin C, a direct vat set starter and probiotic powder, and the mixture ratio is as follows: 5500 parts of fresh goat milk, 110 parts of desalted whey powder, 30 parts of concentrated whey protein, 20 parts of stachyose, 0.3 part of vitamin C, 0.5 part of direct vat set starter and 0.006 part of probiotic powder.

Preferably, the probiotic milk powder consists of fresh goat milk, desalted whey powder, concentrated whey protein, stachyose, vitamin C, a direct vat set starter and probiotic powder, and the mixture ratio is as follows: 6500 parts of fresh goat milk, 245 parts of desalted whey powder, 50 parts of concentrated whey protein, 40 parts of stachyose, 1 parts of vitamin C, 1 part of direct vat set starter and 0.2 part of probiotic powder.

Preferably, the preparation method comprises the following steps:

A. the method comprises the following steps of (1) purchasing fresh milk, desalted whey powder, concentrated whey protein, stachyose and vitamin C in proportion:

B. preparing a direct vat set starter:

b1, mixing the lactobacillus bulgaricus powder and the streptococcus thermophilus powder according to the mass ratio of 2:1, so that the ratio of the viable count of the lactobacillus bulgaricus powder to the streptococcus thermophilus powder is 1:1, and obtaining the direct vat set starter;

C. preparing probiotic powder;

c1, adding 1.2% of lactose, 0.5% of peptone, 0.25% of xylo-oligosaccharide, 0.20% of sodium acetate and 0.22% of ammonium citrate tribasic into 10% of allium macrostemon extract according to the mass-volume ratio to prepare a culture medium;

c2, sterilizing and cooling a culture medium, respectively inoculating activated probiotics, culturing at a constant temperature of 37 ℃ for 24h, freezing and centrifuging to obtain probiotic bacterial paste, adding a composite freeze-drying protective agent which is 1.5 times the weight of the probiotic bacterial paste, performing cold treatment at 10 ℃ for 1.5h, and performing freeze-drying by using a vacuum freeze-drying machine for 24h to obtain probiotic powder;

D. sterilizing and filtering the fresh goat milk;

d1, performing primary filtration sterilization on the fresh milk through a 200-mesh sterilization filter screen;

d2, performing secondary filtration sterilization on the fresh milk through a 400-mesh sterilization filter screen;

E. d, uniformly stirring the filtered and sterilized fresh goat milk obtained in the step D, desalted whey powder, concentrated whey protein, stachyose and vitamin C to obtain a mixture;

F. e, performing high-temperature instantaneous sterilization on the mixture obtained in the step E;

G. f, cooling the mixture subjected to high-temperature sterilization obtained in the step F;

H. g, carrying out reduced pressure concentration on the mixture obtained in the step G after the temperature is reduced to obtain a concentrated milk material;

I. d, spray drying the concentrated milk material obtained in the step H to obtain prebiotics milk powder;

J. adding the direct vat set starter and the probiotic powder, and uniformly stirring again to obtain probiotic milk powder;

K. packaging;

k1, canning and sealing the probiotic milk powder obtained in the step J by a packaging machine.

Preferably, step D is performed by filter sterilization of fresh milk under aseptic conditions, wherein the number of filtration is 2-3.

Preferably, in the step E, when the fresh goat milk, the desalted whey powder, the concentrated whey protein, the stachyose and the vitamin C are uniformly stirred, a stirrer is required, and the rotating speed of the stirrer is 220 r/min.

Preferably, the step F is instantly sterilized by a high-temperature sterilizer at the temperature of 120-150 ℃ for 3-9 s.

Preferably, the mixture sterilized at high temperature in the step G is placed in a sterile environment for natural cooling for 4 hours.

Preferably, in the step I, the concentrated feed is subjected to spray drying by a spray dryer, wherein the air inlet temperature is 150 ℃ and the air outlet temperature is 60 ℃.

Compared with the prior art, the invention has the following beneficial effects:

1. the invention improves the nutrition degree of the milk powder by the accurate proportion of the fresh goat milk, the desalted whey powder, the concentrated whey protein, the stachyose, the vitamin C, the direct vat set starter and the probiotic powder, the fresh goat milk has good nutrition and health care effects on infants, pregnant women, old people and patients, the probiotics powder is added, so that the intestinal absorption and peristalsis are facilitated, the vitamin C is added, the sterilization performance of a human body can be improved, therefore, the nutritional value of the milk powder is fully improved, the problems that the requirements of people on diet are continuously improved along with the continuous improvement of living standard of people, people not only stay on the aspect of satiety and pay more attention to the nutritional function of diet and the safety of food are solved, and the problems that the milk powder is good in quality, generally high in value and really has a health-care effect are few and few in quantity in the current market.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention provides a technical scheme that:

a probiotic formula milk powder and a preparation method thereof, the probiotic milk powder is composed of fresh goat milk, desalted whey powder, concentrated whey protein, stachyose, vitamin C, a direct vat set starter and probiotic powder, and the proportion is as follows: 5500 portions of fresh goat milk and 6500 portions, 110 portions of desalted whey powder and 245 portions, 30 to 50 portions of concentrated whey protein, 20 to 40 portions of stachyose, 0.3 to 1 portion of vitamin C, 0.5 to 1 portion of direct vat set starter and 0.006 to 0.2 portion of probiotic powder.

The probiotic milk powder consists of fresh goat milk, desalted whey powder, concentrated whey protein, stachyose, vitamin C, a direct vat set starter and probiotic powder, and the ratio is as follows: 5500 parts of fresh goat milk, 110 parts of desalted whey powder, 30 parts of concentrated whey protein, 20 parts of stachyose, 0.3 part of vitamin C, 0.5 part of direct vat set starter and 0.006 part of probiotic powder.

The probiotic milk powder consists of fresh goat milk, desalted whey powder, concentrated whey protein, stachyose, vitamin C, a direct vat set starter and probiotic powder, and the ratio is as follows: 6500 parts of fresh goat milk, 245 parts of desalted whey powder, 50 parts of concentrated whey protein, 40 parts of stachyose, 1 parts of vitamin C, 1 part of direct vat set starter and 0.2 part of probiotic powder

The preparation method comprises the following steps:

A. the method comprises the following steps of (1) purchasing fresh milk, desalted whey powder, concentrated whey protein, stachyose and vitamin C in proportion:

B. preparing a direct vat set starter:

b1, mixing the lactobacillus bulgaricus powder and the streptococcus thermophilus powder according to the mass ratio of 2:1, so that the ratio of the viable count of the lactobacillus bulgaricus powder to the streptococcus thermophilus powder is 1:1, and obtaining the direct vat set starter;

C. preparing probiotic powder;

c1, adding 1.2% of lactose, 0.5% of peptone, 0.25% of xylo-oligosaccharide, 0.20% of sodium acetate and 0.22% of ammonium citrate tribasic into 10% of allium macrostemon extract according to the mass-volume ratio to prepare a culture medium;

c2, sterilizing and cooling a culture medium, respectively inoculating activated probiotics, culturing at a constant temperature of 37 ℃ for 24h, freezing and centrifuging to obtain probiotic bacterial paste, adding a composite freeze-drying protective agent which is 1.5 times the weight of the probiotic bacterial paste, performing cold treatment at 10 ℃ for 1.5h, and performing freeze-drying by using a vacuum freeze-drying machine for 24h to obtain probiotic powder;

D. sterilizing and filtering the fresh goat milk;

d1, performing primary filtration sterilization on the fresh milk through a 200-mesh sterilization filter screen;

d2, performing secondary filtration sterilization on the fresh milk through a 400-mesh sterilization filter screen;

E. d, uniformly stirring the filtered and sterilized fresh goat milk obtained in the step D, desalted whey powder, concentrated whey protein, stachyose and vitamin C to obtain a mixture;

F. e, performing high-temperature instantaneous sterilization on the mixture obtained in the step E;

G. f, cooling the mixture subjected to high-temperature sterilization obtained in the step F;

H. g, carrying out reduced pressure concentration on the mixture obtained in the step G after the temperature is reduced to obtain a concentrated milk material;

I. d, spray drying the concentrated milk material obtained in the step H to obtain prebiotics milk powder;

J. adding the direct vat set starter and the probiotic powder, and uniformly stirring again to obtain probiotic milk powder;

K. packaging;

k1, canning and sealing the probiotic milk powder obtained in the step J by a packaging machine.

And D, filtering and sterilizing the fresh milk in an aseptic environment, wherein the filtering times are 2-3.

In this embodiment, specifically, in the step E, when the fresh goat milk, the desalted whey powder, the concentrated whey protein, the stachyose and the vitamin C are uniformly stirred, a stirrer needs to be used, and the rotation speed of the stirrer is 220 rpm.

And F, instantly sterilizing by a high-temperature sterilizer at the temperature of 120-150 ℃ for 3-9 s.

And G, naturally cooling the mixture subjected to high-temperature sterilization for 4 hours in an aseptic environment.

And step I, performing spray drying on the concentrated feed material through a spray dryer, wherein the air inlet temperature is 150 ℃ and the air outlet temperature is 60 ℃.

Example two:

in the first embodiment, the following steps are added:

the probiotic milk powder consists of fresh goat milk, desalted whey powder, concentrated whey protein, stachyose, vitamin C, a direct vat set starter and probiotic powder, and the ratio is as follows: 5500 parts of fresh goat milk, 110 parts of desalted whey powder, 30 parts of concentrated whey protein, 20 parts of stachyose, 0.3 part of vitamin C, 0.5 part of direct vat set starter and 0.006 part of probiotic powder.

The preparation method comprises the following steps:

A. the method comprises the following steps of (1) purchasing fresh milk, desalted whey powder, concentrated whey protein, stachyose and vitamin C in proportion:

B. preparing a direct vat set starter:

b1, mixing the lactobacillus bulgaricus powder and the streptococcus thermophilus powder according to the mass ratio of 2:1, so that the ratio of the viable count of the lactobacillus bulgaricus powder to the streptococcus thermophilus powder is 1:1, and obtaining the direct vat set starter;

C. preparing probiotic powder;

c1, adding 1.2% of lactose, 0.5% of peptone, 0.25% of xylo-oligosaccharide, 0.20% of sodium acetate and 0.22% of ammonium citrate tribasic into 10% of allium macrostemon extract according to the mass-volume ratio to prepare a culture medium;

c2, sterilizing and cooling a culture medium, respectively inoculating activated probiotics, culturing at a constant temperature of 37 ℃ for 24h, freezing and centrifuging to obtain probiotic bacterial paste, adding a composite freeze-drying protective agent which is 1.5 times the weight of the probiotic bacterial paste, performing cold treatment at 10 ℃ for 1.5h, and performing freeze-drying by using a vacuum freeze-drying machine for 24h to obtain probiotic powder;

D. sterilizing and filtering the fresh goat milk;

d1, performing primary filtration sterilization on the fresh milk through a 200-mesh sterilization filter screen;

d2, performing secondary filtration sterilization on the fresh milk through a 400-mesh sterilization filter screen;

E. d, uniformly stirring the filtered and sterilized fresh goat milk obtained in the step D, desalted whey powder, concentrated whey protein, stachyose and vitamin C to obtain a mixture;

F. e, performing high-temperature instantaneous sterilization on the mixture obtained in the step E;

G. f, cooling the mixture subjected to high-temperature sterilization obtained in the step F;

H. g, carrying out reduced pressure concentration on the mixture obtained in the step G after the temperature is reduced to obtain a concentrated milk material;

I. d, spray drying the concentrated milk material obtained in the step H to obtain prebiotics milk powder;

J. adding the direct vat set starter and the probiotic powder, and uniformly stirring again to obtain probiotic milk powder;

K. packaging;

k1, canning and sealing the probiotic milk powder obtained in the step J by a packaging machine.

Example three:

in the second embodiment, the following steps are added:

the probiotic milk powder consists of fresh goat milk, desalted whey powder, concentrated whey protein, stachyose, vitamin C, a direct vat set starter and probiotic powder, and the ratio is as follows: 6500 parts of fresh goat milk, 245 parts of desalted whey powder, 50 parts of concentrated whey protein, 40 parts of stachyose, 1 parts of vitamin C, 1 part of direct vat set starter and 0.2 part of probiotic powder.

The preparation method comprises the following steps:

A. the method comprises the following steps of (1) purchasing fresh milk, desalted whey powder, concentrated whey protein, stachyose and vitamin C in proportion:

B. preparing a direct vat set starter:

b1, mixing the lactobacillus bulgaricus powder and the streptococcus thermophilus powder according to the mass ratio of 2:1, so that the ratio of the viable count of the lactobacillus bulgaricus powder to the streptococcus thermophilus powder is 1:1, and obtaining the direct vat set starter;

C. preparing probiotic powder;

c1, adding 1.2% of lactose, 0.5% of peptone, 0.25% of xylo-oligosaccharide, 0.20% of sodium acetate and 0.22% of ammonium citrate tribasic into 10% of allium macrostemon extract according to the mass-volume ratio to prepare a culture medium;

c2, sterilizing and cooling a culture medium, respectively inoculating activated probiotics, culturing at a constant temperature of 37 ℃ for 24h, freezing and centrifuging to obtain probiotic bacterial paste, adding a composite freeze-drying protective agent which is 1.5 times the weight of the probiotic bacterial paste, performing cold treatment at 10 ℃ for 1.5h, and performing freeze-drying by using a vacuum freeze-drying machine for 24h to obtain probiotic powder;

D. sterilizing and filtering the fresh goat milk;

d1, performing primary filtration sterilization on the fresh milk through a 200-mesh sterilization filter screen;

d2, performing secondary filtration sterilization on the fresh milk through a 400-mesh sterilization filter screen;

E. d, uniformly stirring the filtered and sterilized fresh goat milk obtained in the step D, desalted whey powder, concentrated whey protein, stachyose and vitamin C to obtain a mixture;

F. e, performing high-temperature instantaneous sterilization on the mixture obtained in the step E;

G. f, cooling the mixture subjected to high-temperature sterilization obtained in the step F;

H. g, carrying out reduced pressure concentration on the mixture obtained in the step G after the temperature is reduced to obtain a concentrated milk material;

I. d, spray drying the concentrated milk material obtained in the step H to obtain prebiotics milk powder;

J. adding the direct vat set starter and the probiotic powder, and uniformly stirring again to obtain probiotic milk powder;

K. packaging;

k1, canning and sealing the probiotic milk powder obtained in the step J by a packaging machine.

Example four:

in the third embodiment, the following steps are added:

and D, filtering and sterilizing the fresh milk in an aseptic environment, wherein the filtering times are 2-3.

The preparation method comprises the following steps:

A. the method comprises the following steps of (1) purchasing fresh milk, desalted whey powder, concentrated whey protein, stachyose and vitamin C in proportion:

B. preparing a direct vat set starter:

b1, mixing the lactobacillus bulgaricus powder and the streptococcus thermophilus powder according to the mass ratio of 2:1, so that the ratio of the viable count of the lactobacillus bulgaricus powder to the streptococcus thermophilus powder is 1:1, and obtaining the direct vat set starter;

C. preparing probiotic powder;

c1, adding 1.2% of lactose, 0.5% of peptone, 0.25% of xylo-oligosaccharide, 0.20% of sodium acetate and 0.22% of ammonium citrate tribasic into 10% of allium macrostemon extract according to the mass-volume ratio to prepare a culture medium;

c2, sterilizing and cooling a culture medium, respectively inoculating activated probiotics, culturing at a constant temperature of 37 ℃ for 24h, freezing and centrifuging to obtain probiotic bacterial paste, adding a composite freeze-drying protective agent which is 1.5 times the weight of the probiotic bacterial paste, performing cold treatment at 10 ℃ for 1.5h, and performing freeze-drying by using a vacuum freeze-drying machine for 24h to obtain probiotic powder;

D. sterilizing and filtering the fresh goat milk;

d1, performing primary filtration sterilization on the fresh milk through a 200-mesh sterilization filter screen;

d2, performing secondary filtration sterilization on the fresh milk through a 400-mesh sterilization filter screen;

E. d, uniformly stirring the filtered and sterilized fresh goat milk obtained in the step D, desalted whey powder, concentrated whey protein, stachyose and vitamin C to obtain a mixture;

F. e, performing high-temperature instantaneous sterilization on the mixture obtained in the step E;

G. f, cooling the mixture subjected to high-temperature sterilization obtained in the step F;

H. g, carrying out reduced pressure concentration on the mixture obtained in the step G after the temperature is reduced to obtain a concentrated milk material;

I. d, spray drying the concentrated milk material obtained in the step H to obtain prebiotics milk powder;

J. adding the direct vat set starter and the probiotic powder, and uniformly stirring again to obtain probiotic milk powder;

K. packaging;

k1, canning and sealing the probiotic milk powder obtained in the step J by a packaging machine.

Example five:

in the fourth example, the following steps were added:

and E, uniformly stirring the fresh goat milk, the desalted whey powder, the concentrated whey protein, the stachyose and the vitamin C by using a stirrer at the rotating speed of 220 r/min.

The preparation method comprises the following steps:

A. the method comprises the following steps of (1) purchasing fresh milk, desalted whey powder, concentrated whey protein, stachyose and vitamin C in proportion:

B. preparing a direct vat set starter:

b1, mixing the lactobacillus bulgaricus powder and the streptococcus thermophilus powder according to the mass ratio of 2:1, so that the ratio of the viable count of the lactobacillus bulgaricus powder to the streptococcus thermophilus powder is 1:1, and obtaining the direct vat set starter;

C. preparing probiotic powder;

c1, adding 1.2% of lactose, 0.5% of peptone, 0.25% of xylo-oligosaccharide, 0.20% of sodium acetate and 0.22% of ammonium citrate tribasic into 10% of allium macrostemon extract according to the mass-volume ratio to prepare a culture medium;

c2, sterilizing and cooling a culture medium, respectively inoculating activated probiotics, culturing at a constant temperature of 37 ℃ for 24h, freezing and centrifuging to obtain probiotic bacterial paste, adding a composite freeze-drying protective agent which is 1.5 times the weight of the probiotic bacterial paste, performing cold treatment at 10 ℃ for 1.5h, and performing freeze-drying by using a vacuum freeze-drying machine for 24h to obtain probiotic powder;

D. sterilizing and filtering the fresh goat milk;

d1, performing primary filtration sterilization on the fresh milk through a 200-mesh sterilization filter screen;

d2, performing secondary filtration sterilization on the fresh milk through a 400-mesh sterilization filter screen;

E. d, uniformly stirring the filtered and sterilized fresh goat milk obtained in the step D, desalted whey powder, concentrated whey protein, stachyose and vitamin C to obtain a mixture;

F. e, performing high-temperature instantaneous sterilization on the mixture obtained in the step E;

G. f, cooling the mixture subjected to high-temperature sterilization obtained in the step F;

H. g, carrying out reduced pressure concentration on the mixture obtained in the step G after the temperature is reduced to obtain a concentrated milk material;

I. d, spray drying the concentrated milk material obtained in the step H to obtain prebiotics milk powder;

J. adding the direct vat set starter and the probiotic powder, and uniformly stirring again to obtain probiotic milk powder;

K. packaging;

k1, canning and sealing the probiotic milk powder obtained in the step J by a packaging machine.

Example six:

in the fifth example, the following steps were added:

and F, instantly sterilizing by a high-temperature sterilizer at the temperature of 120-150 ℃ for 3-9 s.

The preparation method comprises the following steps:

A. the method comprises the following steps of (1) purchasing fresh milk, desalted whey powder, concentrated whey protein, stachyose and vitamin C in proportion:

B. preparing a direct vat set starter:

b1, mixing the lactobacillus bulgaricus powder and the streptococcus thermophilus powder according to the mass ratio of 2:1, so that the ratio of the viable count of the lactobacillus bulgaricus powder to the streptococcus thermophilus powder is 1:1, and obtaining the direct vat set starter;

C. preparing probiotic powder;

c1, adding 1.2% of lactose, 0.5% of peptone, 0.25% of xylo-oligosaccharide, 0.20% of sodium acetate and 0.22% of ammonium citrate tribasic into 10% of allium macrostemon extract according to the mass-volume ratio to prepare a culture medium;

c2, sterilizing and cooling a culture medium, respectively inoculating activated probiotics, culturing at a constant temperature of 37 ℃ for 24h, freezing and centrifuging to obtain probiotic bacterial paste, adding a composite freeze-drying protective agent which is 1.5 times the weight of the probiotic bacterial paste, performing cold treatment at 10 ℃ for 1.5h, and performing freeze-drying by using a vacuum freeze-drying machine for 24h to obtain probiotic powder;

D. sterilizing and filtering the fresh goat milk;

d1, performing primary filtration sterilization on the fresh milk through a 200-mesh sterilization filter screen;

d2, performing secondary filtration sterilization on the fresh milk through a 400-mesh sterilization filter screen;

E. d, uniformly stirring the filtered and sterilized fresh goat milk obtained in the step D, desalted whey powder, concentrated whey protein, stachyose and vitamin C to obtain a mixture;

F. e, performing high-temperature instantaneous sterilization on the mixture obtained in the step E;

G. f, cooling the mixture subjected to high-temperature sterilization obtained in the step F;

H. g, carrying out reduced pressure concentration on the mixture obtained in the step G after the temperature is reduced to obtain a concentrated milk material;

I. d, spray drying the concentrated milk material obtained in the step H to obtain prebiotics milk powder;

J. adding the direct vat set starter and the probiotic powder, and uniformly stirring again to obtain probiotic milk powder;

K. packaging;

k1, canning and sealing the probiotic milk powder obtained in the step J by a packaging machine.

Example seven:

in example six, the following steps were added:

and G, naturally cooling the mixture subjected to high-temperature sterilization for 4 hours in an aseptic environment.

The preparation method comprises the following steps:

A. the method comprises the following steps of (1) purchasing fresh milk, desalted whey powder, concentrated whey protein, stachyose and vitamin C in proportion:

B. preparing a direct vat set starter:

b1, mixing the lactobacillus bulgaricus powder and the streptococcus thermophilus powder according to the mass ratio of 2:1, so that the ratio of the viable count of the lactobacillus bulgaricus powder to the streptococcus thermophilus powder is 1:1, and obtaining the direct vat set starter;

C. preparing probiotic powder;

c1, adding 1.2% of lactose, 0.5% of peptone, 0.25% of xylo-oligosaccharide, 0.20% of sodium acetate and 0.22% of ammonium citrate tribasic into 10% of allium macrostemon extract according to the mass-volume ratio to prepare a culture medium;

c2, sterilizing and cooling a culture medium, respectively inoculating activated probiotics, culturing at a constant temperature of 37 ℃ for 24h, freezing and centrifuging to obtain probiotic bacterial paste, adding a composite freeze-drying protective agent which is 1.5 times the weight of the probiotic bacterial paste, performing cold treatment at 10 ℃ for 1.5h, and performing freeze-drying by using a vacuum freeze-drying machine for 24h to obtain probiotic powder;

D. sterilizing and filtering the fresh goat milk;

d1, performing primary filtration sterilization on the fresh milk through a 200-mesh sterilization filter screen;

d2, performing secondary filtration sterilization on the fresh milk through a 400-mesh sterilization filter screen;

E. d, uniformly stirring the filtered and sterilized fresh goat milk obtained in the step D, desalted whey powder, concentrated whey protein, stachyose and vitamin C to obtain a mixture;

F. e, performing high-temperature instantaneous sterilization on the mixture obtained in the step E;

G. f, cooling the mixture subjected to high-temperature sterilization obtained in the step F;

H. g, carrying out reduced pressure concentration on the mixture obtained in the step G after the temperature is reduced to obtain a concentrated milk material;

I. d, spray drying the concentrated milk material obtained in the step H to obtain prebiotics milk powder;

J. adding the direct vat set starter and the probiotic powder, and uniformly stirring again to obtain probiotic milk powder;

K. packaging;

k1, canning and sealing the probiotic milk powder obtained in the step J by a packaging machine.

Example eight:

in example seven, the following steps were added:

and step I, performing spray drying on the concentrated feed material through a spray dryer, wherein the air inlet temperature is 150 ℃ and the air outlet temperature is 60 ℃.

The preparation method comprises the following steps:

A. the method comprises the following steps of (1) purchasing fresh milk, desalted whey powder, concentrated whey protein, stachyose and vitamin C in proportion:

B. preparing a direct vat set starter:

b1, mixing the lactobacillus bulgaricus powder and the streptococcus thermophilus powder according to the mass ratio of 2:1, so that the ratio of the viable count of the lactobacillus bulgaricus powder to the streptococcus thermophilus powder is 1:1, and obtaining the direct vat set starter;

C. preparing probiotic powder;

c1, adding 1.2% of lactose, 0.5% of peptone, 0.25% of xylo-oligosaccharide, 0.20% of sodium acetate and 0.22% of ammonium citrate tribasic into 10% of allium macrostemon extract according to the mass-volume ratio to prepare a culture medium;

c2, sterilizing and cooling a culture medium, respectively inoculating activated probiotics, culturing at a constant temperature of 37 ℃ for 24h, freezing and centrifuging to obtain probiotic bacterial paste, adding a composite freeze-drying protective agent which is 1.5 times the weight of the probiotic bacterial paste, performing cold treatment at 10 ℃ for 1.5h, and performing freeze-drying by using a vacuum freeze-drying machine for 24h to obtain probiotic powder;

D. sterilizing and filtering the fresh goat milk;

d1, performing primary filtration sterilization on the fresh milk through a 200-mesh sterilization filter screen;

d2, performing secondary filtration sterilization on the fresh milk through a 400-mesh sterilization filter screen;

E. d, uniformly stirring the filtered and sterilized fresh goat milk obtained in the step D, desalted whey powder, concentrated whey protein, stachyose and vitamin C to obtain a mixture;

F. e, performing high-temperature instantaneous sterilization on the mixture obtained in the step E;

G. f, cooling the mixture subjected to high-temperature sterilization obtained in the step F;

H. g, carrying out reduced pressure concentration on the mixture obtained in the step G after the temperature is reduced to obtain a concentrated milk material;

I. d, spray drying the concentrated milk material obtained in the step H to obtain prebiotics milk powder;

J. adding the direct vat set starter and the probiotic powder, and uniformly stirring again to obtain probiotic milk powder;

K. packaging;

k1, canning and sealing the probiotic milk powder obtained in the step J by a packaging machine.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.