阅读说明：本技术 一种延缓衰老的核苷酸益生菌胶囊 (Nucleotide probiotic capsule for delaying senescence ) 是由 李勇 丁婷 陈玉松 于 2021-07-30 设计创作，主要内容包括：本发明公开一种延缓衰老的核苷酸益生菌胶囊,属于医药保健技术领域,本发明的核苷酸益生菌胶囊包括胶囊壳和内容物,内容物组分包括4种外源核苷酸和5种益生菌,外源核苷酸包括5’单磷酸胞苷(5’-CMP)、5’单磷酸腺苷(5’-AMP)、5’尿苷酸二钠(5’-UMPNa-(2))和5’鸟苷酸二钠(5’-GMPNa-(2)),益生菌包括嗜酸乳杆菌、植物乳杆菌、干酪乳杆菌等益生菌,益生菌含量均大于1×10～(7)CFU/g。本发明的延缓衰老的核苷酸益生菌胶囊,能够清除体内自由基,抑制自由基对机体的氧化损伤,延长细胞寿命,具有延缓衰老、延年益寿、提高免疫力、调节肠道菌群等多种保健功效,并且携带服用方便,是适合中老年人的保健食品。(The invention discloses a nucleotide probiotic capsule for delaying senescence, which belongs to the technical field of medicine and health care, and comprises a capsule shell and a content, wherein the content comprises 4 exogenous nucleotides and 5 probiotics, the exogenous nucleotides comprise 5 'cytidine monophosphate (5' -CMP), 5 'adenosine monophosphate (5' -AMP) and 5 'disodium uridylate (5' -UMPNa) 2 ) And disodium 5 'guanylate (5' -GMNa) 2 ) The probiotics include Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus casei, etc., and the content of the probiotics is more than 1 × 10 7 CFU/g. The anti-aging nucleotide probiotic capsule can eliminate free radicals in vivo, inhibit oxidative damage of the free radicals to organisms, prolong the service life of cells, has various health-care effects of delaying aging, prolonging life, improving immunity, regulating intestinal flora and the like, is convenient to carry and take, and is a health-care food suitable for middle-aged and elderly people.)

1. The nucleotide probiotic capsule for delaying senescence is characterized by comprising a capsule and contents, wherein the contents comprise a mixture of 4 exogenous nucleotides or sodium salt forms thereof and 5 probiotics, and the weight ratio is as follows: 45-75 parts of nucleotide mixture and 20-30 parts of probiotics.

2. The nucleotide probiotic capsule according to claim 1, characterized in that the weight percentages of the 4 exogenous nucleotides in the content are respectively: 15-45% of CMP, 15-55% of AMP, 6-30% of UMP and 14-35% of GMP.

3. The nucleotide probiotic capsule according to claim 1, wherein the probiotic comprises the following strains in percentage by mass: 17-32% of lactobacillus acidophilus, 17-32% of lactobacillus plantarum, 17-32% of lactobacillus casei, 10-23% of lactobacillus helveticus and 7-19% of pediococcus pentosaceus.

4. The nucleotide probiotic capsule according to any one of claims 1 to 3, characterized in that the weight ratio of the contents is: 10-20 parts of 5 '-cytidine monophosphate (5' -CMP), 20-30 parts of 5 '-adenosine monophosphate (5' -AMP) and 5 '-uridylic acid disodium salt (5' -UMPNa)₂) 5-10 parts of disodium 5 '-guanylate (5' -GMPNA)₂) 10-15 parts of lactobacillus acidophilus, 5-7 parts of lactobacillus plantarum, 5-7 parts of lactobacillus casei, 3-5 parts of lactobacillus helveticus and 2-4 parts of pediococcus pentosaceus.

5. The nucleotide probiotic capsule according to claim 4, characterized in that the weight ratio of the contents is:

10 parts of 5 'cytidine monophosphate, 20 parts of 5' adenosine monophosphate, 5 parts of 5 'uridylate disodium, 10 parts of 5' guanylic acid disodium, 5 parts of lactobacillus acidophilus, 5 parts of lactobacillus plantarum, 5 parts of lactobacillus casei, 3 parts of lactobacillus helveticus and 2 parts of pediococcus pentosaceus;

or the weight ratio of the contents is as follows:

15 parts of 5 'cytidine monophosphate, 25 parts of 5' adenosine monophosphate, 8 parts of 5 'uridylate disodium, 10 parts of 5' guanylic acid disodium, 6 parts of lactobacillus acidophilus, 6 parts of lactobacillus plantarum, 6 parts of lactobacillus casei, 4 parts of lactobacillus helveticus and 3 parts of pediococcus pentosaceus;

or the weight ratio of the contents is as follows:

20 parts of 5 'cytidine monophosphate, 30 parts of 5' adenosine monophosphate, 10 parts of 5 'uridylate disodium, 15 parts of 5' guanylic acid disodium, 7 parts of lactobacillus acidophilus, 7 parts of lactobacillus plantarum, 7 parts of lactobacillus casei, 5 parts of lactobacillus helveticus and 4 parts of pediococcus pentosaceus.

6. The nucleotide probiotic capsule according to claim 1, characterized in that the viable bacteria content of each species in the probiotic is greater than 1 x 10⁷CFU/g。

7. The nucleotide probiotic capsule according to claim 1, wherein the wall material of the capsule is maltodextrin and soybean protein isolate, and the weight ratio of the maltodextrin to the soybean protein isolate is 1: 1-1: 2.

8. The nucleotide probiotic capsule according to claim 7, characterized in that the weight ratio of maltodextrin to soy protein isolate is 1: 1.5-1: 2.

9. The preparation method of the nucleotide probiotic capsule according to any one of claims 1 to 8, characterized by weighing the nucleotide and the probiotic according to a ratio, mixing uniformly to obtain mixed powder, and filling the mixed powder into a hollow capsule to obtain the nucleotide probiotic capsule.

10. Use of the nucleotide probiotic capsule according to any one of claims 1 to 8 for the preparation of a health food and/or a pharmaceutical product for delaying aging.

Technical Field

The invention belongs to the technical field of medicine and health care, and particularly relates to a nucleotide probiotic capsule for delaying senescence.

Background

Aging refers to the process of the human body which shows organization structure change, hypofunction, adaptability and resistance decline and tends to die after the human body grows mature and increases along with time. Aging is a spontaneous and inevitable process as a change in complex life phenomena. With the improvement of living standard of people, China is about to step into an aging society, and aging delaying, health and longevity are the targets pursued by many people, especially women. Pursuing to prolong life is a dream of thousands of years for human beings. Today, prevention and delay of aging has become a hot spot of current research. Research shows that aging is the result of the combined action of various factors, and is related to genetic genes, free radicals, immune dysfunction and other factors. Although the aging is an irresistible natural law in the life activities of everyone, the product for delaying the aging can effectively delay the aging, delay the time of the life, the old and the death, improve the life quality and realize the maximum longevity of the human under the existing conditions by adopting good health care measures and taking the product for delaying the aging.

Nucleotides are important components of cells, are precursors of DNA and RNA, and play important roles in cellular metabolism, protein synthesis, cell division, and the like. Nucleotides become "essential nutrients" for the body under certain conditions, such as disease, rapid growth and development, limited nutrient intake, and hindered endogenous synthesis and expression. Therefore, the nucleotide can be used as a potential substance for delaying senility and prolonging life.

The aging of the body is accompanied by the development of various chronic diseases, and many of the chronic diseases are related to the imbalance of the intestinal flora. Therefore, the intestinal flora is also an important index for influencing the aging of the organism. Probiotics are active microorganisms that have important effects on human health by colonizing in the human body. Researches find that the probiotics have important effects on the aspects of regulating the balance of intestinal flora, promoting nutrient absorption, keeping intestinal health and the like.

At present, a lot of products for delaying senescence exist in the market, but the products have low effect, slow effect and poor effect. Therefore, research and development of a product which is safe and effective in components, free of toxic and side effects and capable of efficiently delaying body aging becomes an important subject to be researched urgently.

Disclosure of Invention

Aiming at the problems, the invention provides a nucleotide probiotic capsule for delaying senescence, which contains 4 exogenous nucleotides and 5 probiotics, has safe and effective components and no toxic or side effect, and has the effects of enhancing immunity, delaying the senescence of organisms and enhancing the vitality of cells.

In order to achieve the purpose, the invention is realized by the following technical scheme:

the nucleotide probiotic capsule for delaying the aging comprises an oval capsule and contents, wherein the contents comprise a mixture of 4 exogenous 5' nucleotides or sodium salt forms thereof and 5 probiotics, and the weight ratio is as follows: 45-75 parts of 5' nucleotide mixture and 20-30 parts of probiotics.

Preferably, the weight percentages of the 4 exogenous 5' nucleotides in the content are respectively: 15-45% of CMP, 15-55% of AMP, 6-30% of UMP and 14-35% of GMP.

Preferably, the content probiotics comprises the following strains in percentage by mass: 17-32% of lactobacillus acidophilus, 17-32% of lactobacillus plantarum, 17-32% of lactobacillus casei, 10-23% of lactobacillus helveticus and 7-19% of pediococcus pentosaceus.

Preferably, the weight ratio of the contents is as follows: 10-20 parts of 5 '-cytidine monophosphate (5' -CMP), 20-30 parts of 5 '-adenosine monophosphate (5' -AMP) and 5 '-uridylic acid disodium salt (5' -UMPNa)₂) 5-10 parts of disodium 5 '-guanylate (5' -GMPNA)₂) 10-15 parts of lactobacillus acidophilus, 5-7 parts of lactobacillus plantarum, 5-7 parts of lactobacillus casei, 3-5 parts of lactobacillus helveticus and 2-4 parts of pediococcus pentosaceus.

Preferably, the composition comprises 10 parts of 5 'cytidine monophosphate, 20 parts of 5' adenosine monophosphate, 5 parts of 5 'disodium uridylate, 10 parts of 5' disodium guanylate, 5 parts of lactobacillus acidophilus, 5 parts of lactobacillus plantarum, 5 parts of lactobacillus casei, 3 parts of lactobacillus helveticus and 2 parts of pediococcus pentosaceus.

Preferably, 15 parts of 5 'cytidine monophosphate, 25 parts of 5' adenosine monophosphate, 8 parts of 5 'disodium uridylate, 10 parts of 5' disodium guanylate, 6 parts of lactobacillus acidophilus, 6 parts of lactobacillus plantarum, 6 parts of lactobacillus casei, 4 parts of lactobacillus helveticus and 3 parts of pediococcus pentosaceus.

Preferably, the composition comprises 20 parts of 5 'cytidine monophosphate, 30 parts of 5' adenosine monophosphate, 10 parts of 5 'disodium uridylate, 15 parts of 5' disodium guanylate, 7 parts of lactobacillus acidophilus, 7 parts of lactobacillus plantarum, 7 parts of lactobacillus casei, 5 parts of lactobacillus helveticus and 4 parts of pediococcus pentosaceus.

Preferably, the viable bacteria content of each strain of the probiotics is more than 1 x 10⁷CFU/g。

Preferably, the wall material of the capsule is maltodextrin and soy protein isolate.

Preferably, the weight ratio of the maltodextrin to the isolated soy protein is 1: 1-1: 2. .

Preferably, the weight ratio of the maltodextrin to the isolated soy protein is 1: 1.5-1: 2.

Preferably, the nucleotide includes natural nucleotides and synthetic nucleotides.

Preferably, the probiotics are probiotic live bacteria freeze-dried powder.

The other aspect of the invention provides a preparation method of the nucleotide probiotic capsule, the contents are weighed according to the proportion and mixed evenly to obtain mixed powder, and the mixed powder is filled into a hollow capsule to obtain the nucleotide probiotic capsule.

The invention also provides application of the nucleotide probiotic capsule in preparation of health-care food and/or medicines for delaying senescence.

Compared with the prior art, the invention has the following beneficial effects:

1. the invention takes dietary nucleotide and probiotics as raw materials, the components are safe and effective, no toxic or side effect exists, the dietary nucleotide and the probiotics are taken in a matching way, the synergistic effect is achieved, the free radicals in vivo can be eliminated, the oxidative damage of the free radicals to organisms is inhibited, the cell life is prolonged, the health-care effects of delaying senility, prolonging the life, improving the immunity, regulating intestinal flora and the like are achieved, the carrying and the taking are convenient, and the health-care food is suitable for middle-aged and elderly people.

2. The nucleotide probiotic capsule has the advantages of simple preparation process, easy operation and good stability, and is beneficial to body health after being taken for a long time.

Detailed Description

The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention. In the following examples, unless otherwise specified, the experimental methods used were all conventional methods, and materials, reagents and the like used were all available from biological or chemical companies.

The nucleotide probiotic capsule for delaying senescence comprises an elliptical capsule shell and contents, wherein the capsule shell comprises maltodextrin and soybean protein isolate; the contents contained 4 dietary nucleotides and various probiotics.

Example 1

A nucleotide probiotic capsule for delaying senescence comprises an elliptical capsule shell and contents, wherein the capsule wall materials are maltodextrin and soybean protein isolate, the weight ratio of the two is 1:1, the contents comprise 4 mixtures in the form of 5' mononucleotide or sodium salt thereof and 5 probiotic viable bacteria freeze-dried powders, and the contents are prepared from the following raw materials in parts by weight: 10 parts of 5 'cytidine monophosphate, 20 parts of 5' adenosine monophosphate, 5 parts of 5 'uridylate disodium, 10 parts of 5' guanylic acid disodium, 5 parts of lactobacillus acidophilus, 5 parts of lactobacillus plantarum, 5 parts of lactobacillus casei, 3 parts of lactobacillus helveticus and 2 parts of pediococcus pentosaceus. The preparation method comprises the steps of uniformly mixing the contents according to the proportion to obtain mixed powder, filling the mixed powder into hollow capsules, and filling 0.2g of the mixed powder into each capsule to obtain the finished product of the nucleotide probiotic capsule.

Example 2

A nucleotide probiotic capsule for delaying senescence comprises an elliptical capsule shell and contents, wherein the capsule wall materials are maltodextrin and soybean protein isolate, the weight ratio of the two is 1:1.5, the contents comprise 4 mixtures in the form of 5' mononucleotide or sodium salt thereof and 5 probiotic viable bacteria freeze-dried powders, and the contents are prepared from the following components in parts by weight: 15 parts of 5 'cytidine monophosphate, 25 parts of 5' adenosine monophosphate, 8 parts of 5 'uridylate disodium, 10 parts of 5' guanylic acid disodium, 6 parts of lactobacillus acidophilus, 6 parts of lactobacillus plantarum, 6 parts of lactobacillus casei, 4 parts of lactobacillus helveticus and 3 parts of pediococcus pentosaceus. The preparation method comprises the steps of uniformly mixing the contents according to the proportion to obtain mixed powder, filling the mixed powder into hollow capsules, and filling 0.2g of the mixed powder into each capsule to obtain the finished product of the nucleotide probiotic capsule.

Example 3

A nucleotide probiotic capsule for delaying senescence comprises an elliptical capsule shell and a content, wherein the capsule wall material is maltodextrin and soybean protein isolate, the weight ratio of the two is 1:2, the content comprises 4 mixtures of 5' mononucleotides or sodium salts thereof and 5 probiotic viable bacteria freeze-dried powders, and the mixture ratio by weight parts is as follows: 20 parts of 5 'cytidine monophosphate, 30 parts of 5' adenosine monophosphate, 10 parts of 5 'uridylate disodium, 15 parts of 5' guanylic acid disodium, 7 parts of lactobacillus acidophilus, 7 parts of lactobacillus plantarum, 7 parts of lactobacillus casei, 5 parts of lactobacillus helveticus and 4 parts of pediococcus pentosaceus. The preparation method comprises the steps of uniformly mixing the contents according to the proportion to obtain mixed powder, filling the mixed powder into hollow capsules, and filling 0.2g of the mixed powder into each capsule to obtain the finished product of the nucleotide probiotic capsule.

Example 4

In order to verify the effect of the nucleotide probiotic capsule on delaying senescence, the experiment firstly examines the antioxidant performance of the compound nucleotide.

Preparing 5' cytidine monophosphate according to the proportion of nucleotides in the nucleotide probiotic capsule in the example 1: 5' adenosine monophosphate: 5' uridylate disodium: the weight ratio of the disodium 5' guanylate is 10: 20: 5: 10, taking 0.1g of mixed nucleotide, dissolving in 100mL of deionized water to prepare a mixed nucleotide mother liquor, and carrying out DPPH free radical clearance measurement and ABTS free radical clearance measurement.

Determination of DPPH radical scavenging Rate:

the mother liquor was diluted 100, 50, 25, 10, 1 fold to prepare gradient dilutions of 10. mu.g/mL, 20. mu.g/mL, 40. mu.g/mL, 100. mu.g/mL, 500. mu.g/mL, respectively.

Respectively placing the above 150 μ L sample and 150 μ L DPPH solution (diluted with anhydrous ethanol) with concentration of 60mg/L in 96-well plate, mixing, storing at 30 deg.C in dark place, and measuring absorbance value under 519nm with microplate reader after 30 min. The DPPH radical clearance equation is as follows:

wherein, A blank is the absorbance of 150 muL deionized water and 150 muL DPPH solution, and A sample is the absorbance of 150 muL sample and 150 muL DPPH solution; IC (integrated circuit)₅₀The concentration of the sample at which DPPH free radical scavenging rate reached 50%.

Determination of ABTS free radical clearance:

(1) the mother liquor was diluted 100, 50, 25, 10, 1 fold to prepare gradient dilutions of 10. mu.g/mL, 20. mu.g/mL, 40. mu.g/mL, 100. mu.g/mL, 500. mu.g/mL, respectively.

(2) A solution of 7mmol/L ABTS and a solution of 140mmol/L potassium persulfate were mixed at a ratio of 62.5: 1, standing overnight at room temperature in the dark to form ABTS stock solution, and diluting with deionized water to obtain 5mmol/L ABTS working solution before use.

(3) 0.15mL of the above diluted solution sample was mixed with 2.85mL of ABTS working solution, and the mixture was left standing at 30 ℃ for 8min, and absorbance was measured at 734nm using deionized water as a blank.

The ABTS free radical clearance equation is as follows:

in the formula, blank A is the absorbance of 0.15mL deionized water and 2.85mL ABTS working solution, and sample A is the absorbance of 0.15mL sample and 2.85mL ABTS working solution. IC (integrated circuit)₅₀Is the concentration of the sample at which the ABTS free radical clearance reaches 50%.

The same detection method is referred to detect the proportion of the nucleotides in the nucleotide probiotic capsule in example 2 to prepare 5' cytidine monophosphate: 5' adenosine monophosphate: 5' uridylate disodium: the weight ratio of the disodium 5' guanylate is 15: 25: 8: 10 and the mixture ratio of the nucleotides in the nucleotide probiotic capsule in example 3, 5' cytidine monophosphate is prepared: 5' adenosine monophosphate: 5' uridylate disodium: the weight ratio of the disodium 5' guanylate is 20: 30: 10: 15, while the antioxidant properties of the mixed nucleotides in the formulation of 5' cytidine monophosphate: 5' adenosine monophosphate: 5' uridylate disodium: the weight ratio of the disodium 5' guanylate is 1: 1: 1:1 as a control experiment, the experimental data are expressed as mean ± standard deviation, and the experimental results are shown in table 1.

TABLE 1 antioxidant Properties of Mixed nucleotides

The results show that the nucleotide mixtures of examples 1-3 all have higher antioxidant properties and can more effectively scavenge free radicals of the body, thereby efficiently delaying the aging of the body and enhancing the vitality of cells, compared with the control group.

Example 5

The metabolism of the body can generate a large amount of oxide free radicals, destroy the cell function, and cause organ aging, thereby causing the aging of the body. Superoxide dismutase (SOD) can scavenge superoxide anion free radical harmful to organism. Glutathione peroxidase (GSH-Px) is an important peroxidase decomposing enzyme widely existing in the body. Can reduce toxic peroxide into non-toxic hydroxyl compound, so as to protect the structure and function of cell membrane from interference and damage of peroxide. Therefore, the SOD and GSH-Px activity can reflect the antioxidant capacity of tissues and is an important index for evaluating the aging of organisms. MDA is a lipid peroxidation product, is closely related to the generation condition of free radicals in a body and the lipid peroxidation degree of body tissue cells, can reflect the oxidative stress damage state of the body tissue, and is also one of important indexes for evaluating aging. In order to further verify the effect of the nucleotide probiotic capsule on delaying aging, animal experiments were carried out on the nucleotide probiotic capsules prepared in examples 1-3, and the effect of the nucleotide probiotic capsule on delaying aging of mice was tested by using a rapid aging tendency SAMP-8 mouse.

First, an experiment was conducted by taking the nucleotide probiotic capsule prepared in example 1 as an example, 40 mice with a rapid aging tendency (SAMP-8 mice, purchased from Beijing Wintolite laboratories Inc.), weighing (20 + -5) G, and randomly divided into 4 groups, i.e., a blank group (the capsule contains only basal feed), a control group 1 (the capsule contains only the same amount and the same ratio of nucleotide probiotic capsule as those of example 1), a control group 2 (the capsule contains only the same amount and the same ratio of probiotic as those of example 1), and a test group (the nucleotide probiotic capsule of example 1), wherein 10 animals in each group were fed adaptively for one week by using American society of Nutrition (AIN-93G) animal feed as basal feed, and the experiments were fed with the corresponding capsules of the blank group, the control group, and the test group, SAMP-8 mice were dosed 2 doses each, 3 times a day for 30 consecutive days, and mouse status was recorded daily.

According to the results, the blank group and the control groups 1-2 showed poor mental state, slow movement and signs of aging with time, while the mice fed with the nucleotide probiotic capsule of the present invention showed good mental and health status.

Examining the last administration, collecting blood from fundus oculi, standing at room temperature, centrifuging to separate serum for later use after blood is completely coagulated, killing mice by dislocation, rapidly separating liver, washing with sodium chloride solution to prepare 10% tissue homogenate, centrifuging, and collecting supernatant of the separated tissue homogenate for later use.

The SOD and GSH-Px activity of mouse serum and liver tissue is determined according to the instructions of the test kit, and the content of malondialdehyde peroxide (MDA) of lipid in mouse serum and liver tissue is determined by thiobarbituric acid spectrophotometry. The experimental data are expressed as means ± standard deviation, and the experimental results are shown in tables 2 to 4.

TABLE 2 MDA, SOD and GSH-Px content data in mice fed the capsules of example 1

TABLE 3 MDA, SOD and GSH-Px content data in mice fed the capsules of example 2

TABLE 4 MDA, SOD and GSH-Px content data in mice fed the capsules of example 3

The results show that compared with the blank control group, the SOD activity and the GSH-Px activity in the serum and the liver tissue of the control group 1-2 mice are improved, and the MDA content in the serum and the liver tissue of the mice is reduced; the numerical values of all indexes of the test group are obviously superior to those of the control group 1-2, and further shows that the anti-aging nucleotide probiotic capsule can synergistically enhance the capability of an organism for clearing free radicals, reduce the degree of tissue cell damage and obviously delay the effect of organism aging without adverse reaction.

Example 6

The test method comprises the following steps: in the same area, 500 female volunteers with aged skin were invited at the same time, aged 45-60 years, and the average age was 54 years.

And (3) inclusion standard: the skin has mild or moderate wrinkles, dry skin, reduced elasticity, pigmentation, dull skin color, etc.; and randomly divided into 10 groups, wherein each group of 50 persons, 10 groups of volunteers respectively correspond to a blank group (the capsule only contains the same amount of glucose), a control group 1 (the capsule only contains the same amount and the same proportion of nucleotide as the nucleotide probiotic capsule in the example 1), a control group 2 (the capsule only contains the same amount and the same proportion of probiotic bacteria as the nucleotide probiotic capsule in the example 1), a control group 3 (the capsule only contains the same amount and the same proportion of nucleotide as the nucleotide probiotic capsule in the example 2), a control group 4 (the capsule only contains the same amount and the same proportion of probiotic bacteria as the nucleotide probiotic capsule in the example 2), a control group 5 (the capsule only contains the same amount and the same proportion of nucleotide as the nucleotide probiotic capsule in the example 3), a control group 6 (the capsule only contains the same amount and the same proportion of probiotic bacteria as the nucleotide probiotic capsule in the example 3), The test group 1 (the nucleotide probiotic capsule of example 1), the test group 2 (the nucleotide probiotic capsule of example 2) and the test group 3 (the nucleotide probiotic capsule of example 3) were performed, and each group of volunteers used the corresponding test product as the only anti-aging and anti-oxidation product, each person took 2 capsules daily for 3 times a day for four weeks, and the volunteers who participated in the test after the test ended fed back the skin change condition and the use feeling, and the results of the effect evaluation are shown in table 5.

TABLE 5 evaluation results of the effects of different capsule products

Group of	The number of participating persons in each group	Facial skin compaction	Is energetic	Memory improvement	Sleep quality improvement
						Test group 1	50	46	45	40	42
Test group 2	50	45	50	41	46
						Test group 3	50	49	47	43	47
Control group 1	50	34	30	32	33
						Control group 2	50	15	20	18	21
Control group 3	50	37	34	30	38
						Control group 4	50	20	23	25	22
Control group 5	50	39	35	37	36
						Control group 6	50	25	24	26	25
Blank group	50	7	13	10	8

The results show that compared with blank groups and control groups 1-6, the nucleotide probiotic capsule can more effectively improve facial skin, energy, memory and sleep quality, thereby achieving the purpose of delaying senility.

It will be apparent to those skilled in the art from this disclosure that many changes and modifications can be made, or equivalents modified, in the embodiments of the invention without departing from the scope of the invention. Therefore, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention shall still fall within the protection scope of the technical solution of the present invention, unless the contents of the technical solution of the present invention are departed.