阅读说明：本技术 一种鸡血白蛋白低聚肽及其制备方法和应用 (Chicken blood albumin oligopeptide and preparation method and application thereof ) 是由 毛新亮 李晓敏 曾瑜 陈晶 袁方 陈小娟 栗原博 古润金 于 2021-08-10 设计创作，主要内容包括：本发明提供一种鸡血白蛋白低聚肽及其制备方法和应用,所述方法利用微生物对鸡血白蛋白进行发酵降解,所述微生物为枯草芽孢杆菌、植物乳杆菌、酵母菌、醋酸杆菌中的一种或两种,所述方法包括以下步骤：(1)将鸡血白蛋白和所述微生物在液体培养基中混合得到发酵体系A,于25～40℃和pH6.0～8.0的条件下发酵得到发酵液；(2)收集步骤(1)中分子量低于3000Da的物质得到所述鸡血白蛋白低聚肽。本发明的鸡血白蛋白低聚肽的制备方法通过生物发酵将鸡血白蛋白降解为小分子多肽,充分利用鸡血中的生物活性物质,进一步拓展鸡血的用途,减少资源浪费,提高其产业经济价值,而且鸡血白蛋白低聚肽具有优异的抗氧化、抗疲劳和提高免疫力的功效。(The invention provides a chicken blood albumin oligopeptide and a preparation method and application thereof, the method utilizes microorganisms to ferment and degrade the chicken blood albumin, the microorganisms are one or two of bacillus subtilis, lactobacillus plantarum, saccharomycetes and bacillus aceticus, and the method comprises the following steps: (1) mixing chicken blood albumin and the microorganism in a liquid culture medium to obtain a fermentation system A, and fermenting at 25-40 ℃ and pH 6.0-8.0 to obtain fermentation liquor; (2) and (2) collecting the substances with the molecular weight lower than 3000Da in the step (1) to obtain the chicken blood albumin oligopeptide. According to the preparation method of the chicken blood albumin oligopeptide, the chicken blood albumin is degraded into small molecular polypeptides through biological fermentation, bioactive substances in the chicken blood are fully utilized, the application of the chicken blood is further expanded, the resource waste is reduced, the industrial economic value of the chicken blood albumin oligopeptide is improved, and the chicken blood albumin oligopeptide has excellent effects of resisting oxidation and fatigue and improving the immunity.)

1. A preparation method of chicken blood albumin oligopeptide is characterized by comprising the following steps:

the chicken blood albumin is fermented and degraded by utilizing microorganisms, wherein the microorganisms are one or two of bacillus subtilis, lactobacillus plantarum, saccharomycetes and bacillus aceticus.

2. The method for preparing the chicken blood albumin oligopeptide according to claim 1, wherein the method comprises the following steps:

(1) mixing chicken blood albumin and the microorganism in a liquid culture medium to obtain a fermentation system A, and fermenting at 25-40 ℃ and pH 6.0-8.0 to obtain fermentation liquor;

(2) and (2) collecting the substances with the molecular weight lower than 3000Da in the step (1) to obtain the chicken blood albumin oligopeptide.

3. The method for preparing the chicken blood albumin oligopeptide according to claim 2, wherein the weight ratio of the chicken blood albumin to the microorganism is (5-12): (0.5-5).

4. The method for preparing chicken blood albumin oligopeptide according to claim 3, wherein the fermentation system A comprises 5-12% of chicken blood albumin by mass and 0.5-5% of microorganisms by mass, and the fermentation time in the step (1) is 18-48 hours.

5. The method for preparing the chicken blood albumin oligopeptide according to claim 4, wherein the chicken blood albumin is at a mass concentration of 7-10% and the microorganism is at a concentration of 3-4% in the fermentation system A.

6. The method for preparing chicken blood albumin oligopeptide according to claim 2, wherein the liquid medium comprises a mixture of peptone and beef extract with a carbon source of 0.5-1% glucose and a nitrogen source of 2-2.5%.

7. The method for preparing the chicken blood albumin oligopeptide according to claim 2, wherein the fermentation is performed at 30-38 ℃ and pH 7.0-7.4 for 24-36 hours in the step (1).

8. The method for preparing the chicken blood albumin oligopeptide according to claim 2, wherein in the step (1), the chicken blood albumin is sterilized before being mixed with the microorganism in the liquid culture medium, and the fermentation broth is sterilized after fermentation;

in the step (2), ultrafiltrate containing polypeptide fragments with molecular weight lower than 3000Da in the fermentation liquor is collected by ultrafiltration, and the ultrafiltrate is subjected to spray drying at the temperature of not more than 180 ℃ to obtain the chicken serum albumin oligopeptide.

9. The chicken blood albumin oligopeptide as defined in any one of claims 1 to 8.

10. The use of the chicken blood albumin oligopeptide as defined in claim 9 in the preparation of antioxidant food or health care products, immunity enhancing food or health care products, and anti-fatigue food or health care products.

Technical Field

The invention relates to the technical field of food processing, in particular to chicken blood albumin oligopeptide and a preparation method and application thereof.

Background

China has huge broiler breeding and slaughtering processing industries, in recent years, the demand of domestic markets is greatly increased, the number of stocked broilers is about 10 billion every year, the stock production is as high as hundreds of billions, and China becomes the second largest chicken producing country in the world after the United states. The chicken blood is a byproduct in the process of slaughtering and processing broilers, the utilization rate of the chicken blood is not high at present in China, part of the chicken blood is prepared into chicken blood powder which is used as animal feed, the added value is low, most of the chicken blood is taken as waste and directly discharged into the environment, so that not only is the resource wasted, but also serious environmental pollution is caused. The chicken blood contains various nutrient substances and bioactive substances, particularly abundant proteins, the protein content in the blood plasma is about 8 percent, the protein content in blood cells is about 35 percent, but the utilization rate and the absorption rate are extremely low.

Disclosure of Invention

The invention aims to overcome the defects in the prior art and provides the chicken blood albumin oligopeptide and the preparation method and the application thereof.

In order to achieve the purpose, the invention adopts the technical scheme that: a method for preparing chicken blood albumin oligopeptide, which comprises the following steps:

the chicken blood albumin is fermented and degraded by utilizing microorganisms, wherein the microorganisms are one or two of bacillus subtilis, lactobacillus plantarum, saccharomycetes and bacillus aceticus.

According to the preparation method of the chicken blood albumin oligopeptide, the chicken blood albumin is degraded into the small molecular polypeptide through biological fermentation, bioactive substances in the chicken blood are fully utilized, the application of the chicken blood is further expanded, the resource waste is reduced, the industrial economic value of the chicken blood albumin oligopeptide is improved, the absorption of the chicken blood albumin oligopeptide in an animal body is better promoted, and the chicken blood albumin oligopeptide has excellent effects of resisting oxidation and fatigue and improving immunity.

Preferably, the microorganism comprises bacillus subtilis, and the microorganism further comprises one of lactobacillus plantarum, yeast and bacillus aceticus.

The inventor finds that the bacillus subtilis fermentation has better hydrolysis capacity on the chicken blood albumin, is beneficial to obtaining high-level small molecular albumin peptide and is more beneficial to animal absorption.

Preferably, the method comprises the steps of:

(1) mixing chicken blood albumin and the microorganism in a liquid culture medium to obtain a fermentation system A, and fermenting at 25-40 ℃ and pH 6.0-8.0 to obtain fermentation liquor;

(2) and (2) collecting the substances with the molecular weight lower than 3000Da in the step (1) to obtain the chicken blood albumin oligopeptide.

The inventor finds that the chicken blood albumin oligopeptide prepared by the method has high yield and low cost through research.

Preferably, the weight ratio of the chicken blood albumin to the microorganism is (5-12): (0.5-5).

The inventor finds that the weight ratio of the chicken blood albumin to the microorganism is (5-12): (0.5-5), the chicken blood albumin oligopeptide prepared by the method has high yield and low cost.

Preferably, in the fermentation system A, the mass concentration of the chicken blood albumin is 5-12%, and the concentration of the microorganism is 0.5-5%.

Preferably, the liquid culture medium comprises a mixture of peptone and beef extract with a carbon source of 0.5% -1% and a nitrogen source of 2% -2.5%.

Preferably, in the fermentation system A, the mass concentration of the chicken blood albumin is 7-10%, and the concentration of the microorganism is 3-4%.

Preferably, in the step (1), the fermentation time is 18-48 hours.

Preferably, in the step (1), the fermentation is carried out at 30-38 ℃ and pH 7.0-7.4 for 24-36 hours.

Preferably, in the step (1), before the chicken blood albumin and the microorganism are mixed in the liquid culture medium, the chicken blood albumin is sterilized, and after fermentation, the fermentation broth is sterilized;

in the step (2), ultrafiltrate containing polypeptide fragments with molecular weight lower than 3000Da in the fermentation liquor is collected by ultrafiltration, and the ultrafiltrate is subjected to spray drying at the temperature of not more than 180 ℃ to obtain the chicken serum albumin oligopeptide.

The method for separating the small molecule active peptide by ultrafiltration has better separation effect, belongs to physical separation, and does not introduce chemical reagents, thereby being safe and non-toxic, and furthest retaining the biological activity of the albumin oligopeptide.

The invention also provides the chicken serum albumin oligopeptide prepared by the preparation method of any one of the chicken serum albumin oligopeptides.

The invention also provides application of the chicken blood albumin oligopeptide in preparing antioxidant food or health-care products, immunity-enhancing food or health-care products and anti-fatigue food or health-care products.

The chicken blood albumin oligopeptide has excellent effects of resisting oxidation and fatigue and improving immunity, has low molecular weight, is beneficial to human body absorption, and has high utilization value in food or health care products.

The invention has the beneficial effects that: the invention provides a chicken blood albumin oligopeptide, a preparation method and application thereof, the preparation method of the chicken blood albumin oligopeptide degrades chicken blood albumin into micromolecular polypeptide through biological fermentation, bioactive substances in chicken blood are fully utilized, the application of the chicken blood is further expanded, the resource waste is reduced, the industrial economic value of the chicken blood albumin oligopeptide is improved, the absorption of the chicken blood albumin oligopeptide in an animal body is better promoted, and the chicken blood albumin oligopeptide has excellent effects of resisting oxidation and fatigue and improving immunity.

Drawings

FIG. 1 is a schematic diagram showing the effect of the chicken blood albumin oligopeptide on in vitro antioxidant activity.

Detailed Description

To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.

Example 1

Preparing a bacillus subtilis seed solution: the bacillus subtilis is purchased from China center for Industrial culture Collection of microorganisms (CICC). The seed liquid was cultured using a liquid culture medium of Bacillus subtilis. The liquid culture medium comprises a mixture of glucose with a carbon source of 1% and peptone and beef extract with a nitrogen source of 2%, the pH value is 6.8-7.0, and the seed liquid is prepared by culturing at 30 ℃ for 12 hours.

In this example, we refer to "the preparation and Property research of Chicken blood Albumin" reported in Xiaoxiang et al (Shantou university's newspaper: Nature science edition, 1998(01):41-45) to prepare crude product of Chicken plasma Albumin. The method comprises the following specific steps: 3.8 percent of fresh chicken blood which is subjected to anticoagulation treatment by sodium citrate is centrifuged at 4000r/min for 10min, and the supernatant is separated to obtain plasma. Adding saturated ammonium sulfate solution with the same volume, stirring uniformly, standing at room temperature for 2h, centrifuging at 3000r/min for 20min, and collecting supernatant. Adjusting pH to 3.8 with HCl (1M), standing at room temperature for 1h at 3000r/min, and centrifuging for 20min to obtain precipitate. Redissolving the precipitate with NaOH (1M), adding deionized water to dilute to 5% (w/v), stirring well, and adding sodium caprylate to make the final concentration 0.0017 g/mL. The pH was adjusted to 5.0 with HCl (1M) and left overnight. The next day, the pH was again adjusted to 5.0, heated at 55 ℃ for 30min, and cooled to room temperature. Adding ammonium sulfate under stirring to reach 40% saturation, and centrifuging at 3000r/min for 20min to obtain supernatant. Adjusting pH to 7.0 with HCl (1M), adding ammonium sulfate under stirring to 75% saturation, adjusting pH to 4.6 with HCl (1M), and centrifuging to obtain precipitate, i.e. chicken blood albumin.

The preparation method of the chicken blood albumin oligopeptide provided by the embodiment of the invention comprises the following steps:

(1) taking 100g of crude chicken blood albumin, adding water to dissolve the substrate with the concentration of 8%, sterilizing for 20 minutes at 121 ℃, cooling to room temperature, adjusting the pH of the solution to 7.5, inoculating 3% of bacillus subtilis into the albumin solution, fermenting for 36 hours at 30 ℃ and 200rpm, sterilizing for 20 minutes at 121 ℃ and stopping fermentation;

(2) cooling to room temperature, centrifuging the fermentation liquid at 4000rpm for 10 minutes, and taking supernatant to obtain albumin zymolysis liquid. And (3) performing ultrafiltration to intercept polypeptide fragments with the molecular weight of less than 3000Da in the glycolysis liquid, and performing spray drying on ultrafiltrate at the inlet temperature of 180 ℃ and the outlet temperature of 80 ℃ to obtain 82.6g of albumin oligopeptide powder.

The albumin oligopeptide obtained in the embodiment is milk white powder, has good water solubility and has no bad flavor. The content of active peptide is 74.6%, the content of water is 4.71%, and the content of ash is less than 2.18%. The results of the peptide molecular weight distribution are shown in Table 1, and the oligopeptide with the molecular weight lower than 1000Da in the chicken plasma albumin oligopeptide accounts for 72.34 percent.

TABLE 1 EXAMPLE 1 molecular weight distribution of chicken blood albumin oligopeptides

Example 2

Preparing lactobacillus plantarum: the lactobacillus plantarum used was purchased from the China center for Industrial culture Collection of microorganisms (CICC). The seed liquid was incubated using a liquid medium. Liquid medium, carbon source 0.5% glucose. And (3) culturing the mixture of peptone, beef extract and yeast powder with the nitrogen source of 2.5 percent at the pH of 6.8-7.0 at 37 ℃ for 12 hours to prepare a seed solution.

The preparation method of the chicken blood albumin oligopeptide provided by the embodiment of the invention comprises the following steps:

(1) taking 100g of crude chicken blood albumin, adding water to dissolve the substrate with the concentration of 10%, sterilizing for 20 minutes at 121 ℃, cooling to room temperature, adjusting the pH of the solution to 7.4, inoculating 5% lactobacillus plantarum into the albumin solution, fermenting for 48 hours at 37 ℃ and 200rpm, sterilizing for 20 minutes at 121 ℃ and stopping fermentation;

(2) cooling to room temperature, centrifuging the fermentation liquid at 4000rpm for 10 minutes, and taking supernatant to obtain albumin zymolysis liquid. And (3) performing ultrafiltration to retain polypeptide fragments with the molecular weight of less than 3000Da in the fermentation liquid. Spray drying the ultrafiltrate at inlet temperature of 180 deg.C and outlet temperature of 80 deg.C to obtain albumin oligopeptide powder 79.6 g.

The albumin oligopeptide obtained in the embodiment is milk white powder, has good water solubility and has no bad flavor. The content of active peptide is 75.4%, the content of water is 6.13%, and the content of ash is less than 3.59%. The results of the peptide molecular weight distribution are shown in Table 2, and the oligopeptide with the molecular weight lower than 1000Da in the chicken plasma albumin oligopeptide accounts for 61.20 percent according to the peptide molecular weight distribution.

Table 2 example 2 molecular weight distribution of chicken blood albumin oligopeptide

Example 3

Preparing yeast: the yeast is purchased from China center for Industrial culture Collection of microorganisms (CICC). The seed liquid was incubated using a liquid medium. Liquid medium, 1.0L of 5 ° Bee wort, 15.0g of agar, natural pH, and cultured at 37 ℃ for 12 hours to prepare seed liquid.

The preparation method of the chicken blood albumin oligopeptide provided by the embodiment of the invention comprises the following steps:

(1) taking 100g of crude chicken blood albumin, adding water to dissolve the substrate with the concentration of 8%, sterilizing for 20 minutes at 121 ℃, cooling to room temperature, adjusting the pH of the solution to 7.0, inoculating 5% of yeast into the albumin solution, fermenting for 48 hours at 37 ℃ and 200rpm, and sterilizing for 20 minutes at 121 ℃ to terminate the fermentation;

(2) cooling to room temperature, centrifuging the fermentation liquid at 4000rpm for 10 minutes, and taking supernatant to obtain albumin zymolysis liquid. And (3) performing ultrafiltration to retain polypeptide fragments with the molecular weight of less than 3000Da in the fermentation liquid. Spray drying the ultrafiltrate at inlet temperature of 180 deg.C and outlet temperature of 80 deg.C to obtain albumin oligopeptide powder 84.2 g.

The albumin oligopeptide obtained in the embodiment is milk white powder, has good water solubility and has no bad flavor. The content of active peptide is 79.6%, the content of water is 3.67%, and the content of ash is less than 2.81%. The results of the peptide molecular weight distribution are shown in Table 3, and it can be found that 63.02% of the chicken plasma albumin oligopeptide with the molecular weight lower than 1000 Da.

Table 3 example 3 molecular weight distribution of chicken blood albumin oligopeptide

Example 4

Preparing acetobacter: the acetobacter is purchased from China center for Industrial culture Collection of microorganisms (CICC). The seed liquid was incubated using a liquid medium. Liquid culture medium, glucose 100.0g, yeast extract 10.0g, CaCO₃20.0g, agar 15.0g, and distilled water 1.0L, pH 6.8, cultured at 37 ℃ for 12 hours to prepare a seed solution.

The preparation method of the chicken blood albumin oligopeptide provided by the embodiment of the invention comprises the following steps:

(1) taking 100g of crude chicken blood albumin, adding water to dissolve the substrate with the concentration of 12%, sterilizing for 20 minutes at 121 ℃, cooling to room temperature, adjusting the pH of the solution to 6.5, inoculating 5% acetobacter into the albumin solution, fermenting for 48 hours at 37 ℃ and 200rpm, sterilizing for 20 minutes at 121 ℃ and stopping fermentation;

(2) cooling to room temperature, centrifuging the fermentation liquid at 4000rpm for 10 minutes, and taking supernatant to obtain albumin zymolysis liquid. And (3) performing ultrafiltration to retain polypeptide fragments with the molecular weight of less than 3000Da in the fermentation liquid. And (3) carrying out spray drying on the ultrafiltrate at the inlet temperature of 180 ℃ and the outlet temperature of 80 ℃ to obtain 78.2g of albumin oligopeptide powder.

The albumin oligopeptide obtained in the embodiment is milk white powder, has good water solubility and has no bad flavor. The content of active peptide is 81.6%, the content of water is 4.37%, and the content of ash is less than 3.08%. The results of the peptide molecular weight distribution are shown in Table 4, and it can be found that 67.37% of the chicken plasma albumin oligopeptide with the molecular weight lower than 1000 Da.

Table 4 example 4 molecular weight distribution of chicken blood albumin oligopeptides

Example 5

As can be seen from examples 1-4, after molecular weight analysis, the content of oligopeptides with molecular weight below 1000Da in the chicken blood albumin polypeptide prepared by fermentation of Bacillus subtilis, Lactobacillus plantarum, yeast and Acetobacter is 72.34%, 61.20%, 63.02% and 67.37%, respectively, which indicates that the fermentation of Bacillus subtilis has better hydrolysis capability on chicken blood albumin, and is beneficial to obtaining higher-level small molecular albumin peptide.

The in vitro antioxidant activity of the chicken blood albumin oligopeptide in example 1 was tested by ORAC method.

The antioxidant activity of the oligopeptide in vitro was tested by an Oxidative Radical Absorption Capacity (ORAC) experiment. The experimental groups are respectively AAPH^-Group (blank), AAPH⁺Group (positive control), Trolox (water-soluble vitamin E) group (standard), and test sample group. PBS, test sample, fluorescein sodium and AAPH were added to a 96-well plate and rapidly placed in a fluorescence microplate reader to detect changes in fluorescence signal (at 485nm excitation wavelength and 535nm emission wavelength) within 2h at 37 ℃. The fluorescein sodium is easily attacked and quenched by free radicals generated by AAPH, so that the antioxidant capacity of the tested sample can be reflected by detecting the change of fluorescence intensity. The in vitro antioxidant activity and ORAC value of the chicken blood albumin oligopeptide are shown in figure 1. As can be seen from FIG. 1, the chicken blood albumin oligopeptide has good antioxidant activity, and the ORAC value increases with the increase of the concentration (2.5mg/mL albumin oligopeptide reduction equivalent ORAC value: 342.11; 5.0mg/mL albumin oligopeptide reduction equivalent ORAC value: 692.14).

Example 6

The effect of the chicken blood albumin oligopeptide on the immune function of immunosuppressed mice in example 1.

Kunming mice were randomly divided into a normal group, a Cyclophosphamide (CTX) model group, an albumin oligopeptide low dose group (25mg/kg), an albumin oligopeptide medium dose group (50mg/kg) and an albumin oligopeptide high dose group (100 mg/kg). One week after the animals were acclimatized, the animals were continuously gavaged with albumin oligopeptide for 21 days. From the 19 th day of administration, except the normal group, each group was intraperitoneally injected with 80mg/kg cyclophosphamide daily to establish an immunosuppressive mouse model. After the last administration, the mice fasting for 24h, weighing the weight, taking blood from the orbit, and taking the thymus and spleen for experimental detection.

The thymus is the central immune organ of the body, an important place for the differentiation and maturation of T cells. The spleen is an important peripheral immune organ of the body, where T and B lymphocytes colonize and proliferate. The thymus index and spleen index can reflect the immune function of the body. Immunosuppressive agents such as cyclophosphamide inhibit lymphocyte differentiation, reduce the number of lymphocytes in immune organs such as thymus and spleen, and further lead to reduction in the weight of immune organs. Therefore, the influence of the test object on the body immunity can be detected by observing the thymus index and the spleen index. The mouse thymus and spleen were weighed and the corresponding organ index (%) -organ weight (g)/body weight (g) was calculated. Mouse thymus and spleen indices are shown in table 5. From the results shown in Table 5, it was found that each dose group of the oligopeptide increased the thymus index and spleen index of the mice to some extent, as compared with the cyclophosphamide group.

TABLE 5 Effect of Albumin oligopeptide on thymus index and spleen index in immunosuppressed mice

P <0.05 compared to placebo; compared to the cyclophosphamide model group, # P < 0.05.

Example 7

Effect of chicken blood albumin polypeptide on exercise-induced fatigue mice in example 1.

Kunming mice were randomly divided into a normal group, an albumin oligopeptide low dose group (25mg/kg), an albumin oligopeptide medium dose group (50mg/kg) and an albumin oligopeptide high dose group (100 mg/kg). One week after the animals were acclimatized, the animals were continuously gavaged with albumin oligopeptide for 21 days. 30min after the last administration, the tail root of the mouse is given a 5% weight lead skin load and placed in a 30cm swimming box for exhaustive swimming experiments, and the water temperature is controlled at 25 +/-1 ℃. The time from the beginning of swimming to the appearance of the exhaustion state is recorded as the mouse exhaustion swimming time. And sampling the blood and the liver of the mouse for subsequent experimental determination.

Weight swimming is a forced whole-body energy-wasting activity, and the swimming time of the weight swimming can reflect the anti-fatigue capability of a mouse. The mouse swimming time is shown in table 6. From the results in table 6, it can be seen that the low, medium and high doses of albumin oligopeptide significantly prolonged the swimming time of mice. Hepatic glycogen is an important energy storage mode of the body and can be decomposed into glucose to maintain the stability of blood sugar. During exhaustion exercise, hepatic glycogen is catabolized and converted into energy for the body to use, and further the hepatic glycogen content is reduced. Mouse liver glycogen is shown in Table 6. From the results in table 6, it can be seen that low, medium and high doses of albumin oligopeptide significantly increased the level of liver glycogen in mice. Urea nitrogen is the end product of catabolism of amino acids via the ornithine cycle. When energy generated by sugar metabolism and fat metabolism cannot meet the needs of the organism due to long-term strenuous exercise, protein and amino acid participate in metabolism to provide energy. The mouse urea nitrogen is shown in table 6. From the results in table 6, it can be seen that the low, medium and high doses of albumin oligopeptide significantly reduced the urea nitrogen level in mice. The organism is in anaerobic respiration state during exhaustion exercise, and rapid energy supply is carried out through glycolysis. Glucose is metabolized into lactic acid, which is accumulated in muscle tissue, resulting in the decrease of muscle motor ability and fatigue of the body. The blood lactate of the mice is shown in table 6. From the results in table 6, it can be seen that the low, medium and high doses of albumin oligopeptide significantly reduced the blood lactic acid level of mice. In conclusion, the albumin oligopeptide has better anti-fatigue effect.

TABLE 6 Effect of Albumin oligopeptide on swimming time, serum Urea Nitrogen, liver glycogen and blood lactate content in exhaust exercise mice

P <0.05 compared to the blank control group.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.