阅读说明：本技术 一种防虫害的复合微生物菌剂及其应用 (Insect-resistant compound microbial agent and application thereof ) 是由 祝春华 祝伟倩 朱永杰 何孟夏 孙辉 于 2021-06-16 设计创作，主要内容包括：本发明适用微生物菌剂技术领域,提供一种防虫害的复合微生物菌剂及其应用,本发明中的复合微生物菌剂由多粘芽孢杆菌、绿僵菌、苏云金芽孢杆菌、链霉菌、防护剂、运载体和膨润土组成的粉剂,本发明中的复合微生物菌剂对根结线虫具有良好的防治和灭杀效果,本发明中通过绿僵菌和苏云金芽孢杆菌的联合使用显著的提高了对鳞翅目、双翅目和鞘翅目昆虫的防治效果,利于植物的生长,解决了现有的药剂抗性大,不利于长期灭杀鳞翅目、双翅目和鞘翅目昆虫,易使得杀鳞翅目、双翅目和鞘翅目昆虫反复出现的问题。(The invention is suitable for the technical field of microbial agents, and provides a compound microbial agent for preventing insect pests and an application thereof.)

1. The compound microbial agent for preventing insect pests is characterized by comprising the following components in parts by weight:

30-50 parts of bacillus polymyxa liquid, 20-40 parts of metarhizium anisopliae liquid, 20-40 parts of bacillus thuringiensis liquid, 10-30 parts of streptomycete liquid, 20-40 parts of protective agent, 60-80 parts of carrier and 80-100 parts of bentonite.

2. The compound microbial agent for preventing insect pests according to claim 1, which comprises the following components in parts by weight:

35-50 parts of bacillus polymyxa, 25-40 parts of metarhizium anisopliae, 25-40 parts of bacillus thuringiensis, 15-30 parts of streptomyces, 25-40 parts of a protective agent, 65-80 parts of a carrier and 85-100 parts of bentonite.

3. The compound microbial agent for preventing insect pests according to claim 2, which comprises the following components in parts by weight:

35-45 parts of bacillus polymyxa, 25-35 parts of metarhizium anisopliae, 25-35 parts of bacillus thuringiensis, 15-25 parts of streptomyces, 25-35 parts of protective agent, 65-75 parts of carrier and 85-95 parts of bentonite.

4. The complex microbial inoculant according to claim 1, wherein the protective agent is a combination of hydrolyzed collagen, chitin, cyclodextrin and gelatin.

5. The compound microbial inoculant for pest control according to claim 4, wherein the mass ratio of the hydrolyzed collagen, the chitin, the cyclodextrin and the gelatin is 1.5-1.7: 1-1.2: 1.6-1.8: 1.

6. the compound microbial inoculant for pest control according to claim 5, wherein the mass ratio of the hydrolyzed collagen, the chitin, the cyclodextrin and the gelatin is 1.55-1.65: 1.05-1.15: 1.65-1.75: 1.

7. a method for preparing a compound microbial preparation for preventing insect pests according to any one of claims 1 to 6, which comprises the following steps:

crushing the carrier, and preparing carrier powder into slurry with the mass percent of 70-80%; liquid fermentation culture of bacillus polymyxa, metarhizium anisopliae, bacillus thuringiensis and streptomycete is carried out, bacillus polymyxa, metarhizium anisopliae, bacillus thuringiensis and streptomycete are obtained respectively, then the obtained bacillus polymyxa, metarhizium anisopliae, bacillus thuringiensis and streptomycete are respectively subjected to rotary culture, each bacterium liquid is taken out, a detection agent is used for separating live bacteria and dead bacteria in each bacterium, and live bacteria are taken out;

weighing corresponding parts by weight of bacillus polymyxa liquid, metarhizium liquid, bacillus thuringiensis liquid and streptomycete liquid, respectively carrying out spray drying on the respective liquid to prepare powdery bacterium powder, then placing the bacterium powders together for mixing, putting the mixed bacterium powder into the carrier slurry prepared in the step (1), stirring and mixing, adding a protective agent after stirring uniformly, adding bentonite after stirring uniformly, and stirring uniformly to obtain the product.

8. The method according to claim 7, wherein the amount of viable bacteria in the product is 2-3 hundred million/g in step (2).

9. The use of a compound microbial inoculant according to any one of claims 1 to 7 for the control of lepidopteran, dipteran and coleopteran insects.

Technical Field

The invention belongs to the technical field of microbial agents, and particularly relates to a compound microbial agent for preventing insect pests and an application thereof.

Background

The continuous development of society, agriculture also obtains quick development, needs to use a large amount of pesticides and chemical fertilizers in the farming process, because contain a large amount of chemical agents in pesticides and the chemical fertilizers, makes the soil nutrition unbalance easily to can produce the sick worm, wherein the root knot nematode is the insect pest that often appears in the agriculture.

At present, pesticides are generally adopted for preventing and treating plant meloidogyne, the pesticide resistance of the meloidogyne is improved by using the pesticides for a long time, the pesticide effect is weakened, meanwhile, part of the pesticides can cause certain pollution to the environment, and the existing method for preventing and treating insect pests by adopting a microbial agent is a method for expelling the insect pests or harmful bacteria by utilizing substances which can resist the insect pests or the harmful bacteria and can be generated in the metabolic process of the microbial agent. The existing chemical agents have high resistance, are not beneficial to killing lepidoptera, diptera and coleoptera for a long time, and are easy to cause the lepidoptera, diptera and coleoptera to be killed repeatedly.

Disclosure of Invention

The invention aims to provide a compound microbial agent for preventing insect pests and application thereof, so as to solve the problems in the background technology.

In order to achieve the aim, the invention provides a compound microbial agent for preventing insect pests, which comprises the following components in parts by weight:

30-50 parts of bacillus polymyxa liquid, 20-40 parts of metarhizium anisopliae liquid, 20-40 parts of bacillus thuringiensis liquid, 10-30 parts of streptomycete liquid, 20-40 parts of protective agent, 60-80 parts of carrier and 80-100 parts of bentonite.

Preferably, the composition comprises the following components in parts by weight:

35-50 parts of bacillus polymyxa, 25-40 parts of metarhizium anisopliae, 25-40 parts of bacillus thuringiensis, 15-30 parts of streptomyces, 25-40 parts of a protective agent, 65-80 parts of a carrier and 85-100 parts of bentonite.

Preferably, the composition comprises the following components in parts by weight:

35-45 parts of bacillus polymyxa, 25-35 parts of metarhizium anisopliae, 25-35 parts of bacillus thuringiensis, 15-25 parts of streptomyces, 25-35 parts of protective agent, 65-75 parts of carrier and 90-100 parts of bentonite.

Preferably, the protective agent is a composition between hydrolyzed collagen, chitin, cyclodextrin and gelatin.

Preferably, the mass ratio of the hydrolyzed collagen, the chitin, the cyclodextrin and the gelatin is 1.5-1.7: 1-1.2: 1.6-1.8: 1.

preferably, the mass ratio of the hydrolyzed collagen, the chitin, the cyclodextrin and the gelatin is 1.55-1.65: 1.05-1.15: 1.65-1.75: 1.

preferably, the carrier is diatomaceous earth.

The preparation method of the compound microbial agent for preventing insect pests specifically comprises the following steps:

(1) crushing the carrier, and preparing carrier powder into slurry with the mass percent of 70-80%; liquid fermentation culture of bacillus polymyxa, metarhizium anisopliae, bacillus thuringiensis and streptomycete is carried out, bacillus polymyxa, metarhizium anisopliae, bacillus thuringiensis and streptomycete are obtained respectively, then the obtained bacillus polymyxa, metarhizium anisopliae, bacillus thuringiensis and streptomycete are respectively subjected to rotary culture, each bacterium liquid is taken out, a detection agent is used for separating live bacteria and dead bacteria in each bacterium, and live bacteria are taken out;

(2) weighing corresponding parts by weight of bacillus polymyxa liquid, metarhizium liquid, bacillus thuringiensis liquid and streptomycete liquid, respectively carrying out spray drying on the respective liquid to prepare powdery bacterium powder, then placing the bacterium powders together for mixing, putting the mixed bacterium powder into the carrier slurry prepared in the step (1), stirring and mixing, adding a protective agent after stirring uniformly, adding bentonite after stirring uniformly, and stirring uniformly to obtain the product.

Preferably, in the step (2), the number of effective viable bacteria in the product is 2-3 hundred million/gram.

Preferably, the application of the compound microbial agent for preventing insect pests in preventing and controlling lepidoptera, diptera and coleoptera is provided.

In summary, due to the adoption of the technical scheme, the method has the following beneficial effects:

the invention provides a compound microbial agent for preventing insect pests and application thereof, wherein the compound microbial agent has good prevention, control and killing effects on lepidoptera, diptera and coleopteran insects, the prevention and control effects on the lepidoptera, diptera and coleopteran insects are obviously improved by combined use of metarhizium and bacillus thuringiensis, the compound microbial agent can effectively prevent and control the lepidoptera, diptera and coleopteran insects for a long time, and is beneficial to the growth of plants.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

The existing pesticide has high resistance, is not beneficial to killing lepidoptera, diptera and coleoptera for a long time, and is easy to cause the lepidoptera, diptera and coleoptera killing to appear repeatedly.

Example 1

A preparation method of a compound microbial agent for preventing insect pests specifically comprises the following steps:

(1) crushing 60g of carrier, namely diatomite, sieving the carrier by a 150-mesh sieve, and preparing 70% slurry of carrier powder by mass percent; culturing the bacillus polymyxa, the metarhizium anisopliae, the bacillus thuringiensis and the streptomycete by liquid fermentation, wherein the main components in a culture medium of the liquid fermentation are 3g of beef extract, 10g of peptone, 5g of NaCl, 1000mL of distilled water and 7.4 of pH, respectively obtaining bacillus polymyxa, metarhizium anisopliae, bacillus thuringiensis and streptomycete, respectively carrying out rotary culture on the obtained bacillus polymyxa, metarhizium anisopliae, bacillus thuringiensis and streptomycete to enable the rotating speed to be 300r/min and rotate for 15 minutes, then taking out each bacterial liquid, separating live bacteria and dead bacteria in each bacterial strain by trypan blue, and taking out the live bacteria;

(2) weighing 30g of bacillus polymyxa liquid, 20g of metarhizium anisopliae liquid, 20g of bacillus thuringiensis liquid and 10g of streptomycete liquid, respectively carrying out spray drying on the liquid to prepare powdery bacterium powder, then placing the bacterium powder together for mixing, placing the mixed bacterium powder into the carrier liquid prepared in the step (1), stirring and mixing, adding 20g of protective agent after uniformly stirring, wherein the protective agent is a composition among hydrolyzed collagen, chitin, cyclodextrin and gelatin, and the mass ratio among the hydrolyzed collagen, the chitin, the cyclodextrin and the gelatin is 1.5: 1: 1.6: 1; after being stirred uniformly, 80g of bentonite is added, and the product can be obtained after being stirred uniformly, wherein the number of effective viable bacteria in the product is 2 to 3 hundred million/g.

Example 2

A preparation method of a compound microbial agent for preventing insect pests specifically comprises the following steps:

(1) crushing 80g of carrier, namely diatomite, sieving the carrier by a 150-mesh sieve, and preparing slurry with the carrier powder in a mass percent of 80%; liquid fermentation culture of bacillus polymyxa, destruxinus destructor, bacillus thuringiensis and streptomycete is carried out, bacillus polymyxa, destruxinus, bacillus thuringiensis and streptomycete are obtained respectively, then the obtained bacillus polymyxa, destruxinus, bacillus thuringiensis and streptomycete are respectively subjected to rotary culture, the rotating speed is 400r/min, the rotation is carried out for 20 minutes, then each bacterium liquid is taken out, live bacteria and dead bacteria in each bacterium are separated by trypan blue, and the live bacteria are taken out;

(2) weighing 50g of bacillus polymyxa liquid, 40g of metarhizium anisopliae liquid, 40g of bacillus thuringiensis liquid and 30g of streptomycete liquid, respectively carrying out spray drying on the liquid to prepare powdery bacterium powder, then placing the bacterium powder together for mixing, placing the mixed bacterium powder into the carrier liquid prepared in the step (1), stirring and mixing, adding 40g of protective agent after uniformly stirring, wherein the protective agent is a composition among hydrolyzed collagen, chitin, cyclodextrin and gelatin, and the mass ratio among the hydrolyzed collagen, the chitin, the cyclodextrin and the gelatin is 1.7: 1.2: 1.8: 1; after being stirred uniformly, 100g of bentonite is added, and the product can be obtained after being stirred uniformly, wherein the number of effective viable bacteria in the product is 2 to 3 hundred million/g.

Example 3

A preparation method of a compound microbial agent for preventing insect pests specifically comprises the following steps:

(1) crushing 65g of a carrier, namely diatomite, sieving the carrier through a 150-mesh sieve, and preparing 75% slurry of carrier powder in percentage by mass; liquid fermentation culture of bacillus polymyxa, destruxinus destructor, bacillus thuringiensis and streptomycete is carried out, bacillus polymyxa, destruxinus, bacillus thuringiensis and streptomycete are obtained respectively, then the obtained bacillus polymyxa, destruxinus, bacillus thuringiensis and streptomycete are respectively subjected to rotary culture, the rotating speed is 350r/min, the rotating speed is 18 minutes, then each bacterium liquid is taken out, live bacteria and dead bacteria in each bacterium are separated by trypan blue, and the live bacteria are taken out;

(2) weighing 35g of bacillus polymyxa liquid, 25g of metarhizium anisopliae liquid, 25g of bacillus thuringiensis liquid and 15g of streptomycete liquid, respectively carrying out spray drying on the liquid to prepare powdery bacterium powder, then placing the bacterium powder together for mixing, placing the mixed bacterium powder into the carrier liquid prepared in the step (1), stirring and mixing, adding 25g of protective agent after uniformly stirring, wherein the protective agent is a composition among hydrolyzed collagen, chitin, cyclodextrin and gelatin, and the mass ratio among the hydrolyzed collagen, the chitin, the cyclodextrin and the gelatin is 1.5: 1: 1.6: 1; after being stirred uniformly, 85g of bentonite is added, and the product can be obtained after being stirred uniformly, wherein the number of effective viable bacteria in the product is 2 to 3 hundred million/g.

Example 4

A preparation method of a compound microbial agent for preventing insect pests specifically comprises the following steps:

(1) crushing 70g of carrier, namely diatomite, sieving the carrier by a 150-mesh sieve, and preparing slurry with the carrier powder at a mass percent of 75%; liquid fermentation culture of bacillus polymyxa, destruxinus destructor, bacillus thuringiensis and streptomycete is carried out, bacillus polymyxa, destruxinus, bacillus thuringiensis and streptomycete are obtained respectively, then the obtained bacillus polymyxa, destruxinus, bacillus thuringiensis and streptomycete are respectively subjected to rotary culture, the rotating speed is 350r/min, the rotating speed is 18 minutes, then each bacterium liquid is taken out, live bacteria and dead bacteria in each bacterium are separated by trypan blue, and the live bacteria are taken out;

(2) weighing 40g of bacillus polymyxa liquid, 30g of metarhizium anisopliae liquid, 30g of bacillus thuringiensis liquid and 20g of streptomycete liquid, respectively carrying out spray drying on the liquid to prepare powdery bacterium powder, then placing the bacterium powder together for mixing, placing the mixed bacterium powder into the carrier liquid prepared in the step (1), stirring and mixing, adding 30g of protective agent after uniformly stirring, wherein the protective agent is a composition among hydrolyzed collagen, chitin, cyclodextrin and gelatin, and the mass ratio among the hydrolyzed collagen, the chitin, the cyclodextrin and the gelatin is 1.5: 1: 1.6: 1; after being stirred uniformly, 95g of bentonite is added, and the product can be obtained after being stirred uniformly, wherein the number of effective viable bacteria in the product is 2 to 3 hundred million/g.

Example 5

A preparation method of a compound microbial agent for preventing insect pests specifically comprises the following steps:

(1) crushing 75g of carrier, namely diatomite, sieving the carrier by a 150-mesh sieve, and preparing slurry with 75% of carrier powder by mass percent; liquid fermentation culture of bacillus polymyxa, destruxinus destructor, bacillus thuringiensis and streptomycete is carried out, bacillus polymyxa, destruxinus, bacillus thuringiensis and streptomycete are obtained respectively, then the obtained bacillus polymyxa, destruxinus, bacillus thuringiensis and streptomycete are respectively subjected to rotary culture, the rotating speed is 350r/min, the rotating speed is 18 minutes, then each bacterium liquid is taken out, live bacteria and dead bacteria in each bacterium are separated by trypan blue, and the live bacteria are taken out;

(2) weighing 45g of bacillus polymyxa liquid, 35g of metarhizium anisopliae liquid, 35g of bacillus thuringiensis liquid and 25g of streptomycete liquid, respectively carrying out spray drying on the liquid to prepare powdery bacterium powder, then placing the bacterium powder together for mixing, placing the mixed bacterium powder into the carrier liquid prepared in the step (1), stirring and mixing, adding 35g of protective agent after uniformly stirring, wherein the protective agent is a composition among hydrolyzed collagen, chitin, cyclodextrin and gelatin, and the mass ratio among the hydrolyzed collagen, the chitin, the cyclodextrin and the gelatin is 1.5: 1: 1.6: 1; after being stirred uniformly, 90g of bentonite is added, and the product can be obtained after being stirred uniformly, wherein the number of effective viable bacteria in the product is 2 to 3 hundred million/g.

Example 6

A preparation method of a compound microbial agent for preventing insect pests specifically comprises the following steps:

(1) crushing 70g of carrier, namely diatomite, sieving the carrier by a 150-mesh sieve, and preparing slurry with the carrier powder at a mass percent of 75%; liquid fermentation culture of bacillus polymyxa, destruxinus destructor, bacillus thuringiensis and streptomycete is carried out, bacillus polymyxa, destruxinus, bacillus thuringiensis and streptomycete are obtained respectively, then the obtained bacillus polymyxa, destruxinus, bacillus thuringiensis and streptomycete are respectively subjected to rotary culture, the rotating speed is 350r/min, the rotating speed is 18 minutes, then each bacterium liquid is taken out, live bacteria and dead bacteria in each bacterium are separated by trypan blue, and the live bacteria are taken out;

(2) weighing 40g of bacillus polymyxa liquid, 30g of metarhizium anisopliae liquid, 30g of bacillus thuringiensis liquid and 20g of streptomycete liquid, respectively carrying out spray drying on the liquid to prepare powdery bacterium powder, then placing the bacterium powder together for mixing, putting the mixed bacterium powder into the carrier liquid prepared in the step (1), stirring and mixing, adding 30g of protective agent after uniformly stirring, wherein the protective agent is a composition among hydrolyzed collagen, chitin, cyclodextrin and gelatin, and the mass ratio among the hydrolyzed collagen, the chitin, the cyclodextrin and the gelatin is 1.6: 1: 1.6: 1; after being stirred uniformly, 90g of bentonite is added, and the product can be obtained after being stirred uniformly, wherein the number of effective viable bacteria in the product is 2 to 3 hundred million/g.

Example 7

The preparation method of this example is the same as that of example 6, and the data are partially the same, except that the mass ratio of hydrolyzed collagen, chitin, cyclodextrin and gelatin in the protective agent in this example is 1.6: 1: 1.65: 1.

example 8

The preparation method of this example is the same as that of example 6, and the data are partially the same, except that the mass ratio of hydrolyzed collagen, chitin, cyclodextrin and gelatin in the protective agent in this example is 1.6: 1: 1.7: 1.

example 9

The preparation method of this example is the same as that of example 6, and the data are partially the same, except that the mass ratio of hydrolyzed collagen, chitin, cyclodextrin and gelatin in the protective agent in this example is 1.6: 1: 1.75: 1.

example 10

The preparation method of this example is the same as that of example 6, and the data are partially the same, except that the mass ratio of hydrolyzed collagen, chitin, cyclodextrin and gelatin in the protective agent in this example is 1.6: 1: 1.8: 1.

example 11

A preparation method of a compound microbial agent for preventing insect pests specifically comprises the following steps:

(1) crushing 70g of carrier, namely diatomite, sieving the carrier by a 150-mesh sieve, and preparing slurry with the carrier powder at a mass percent of 75%; liquid fermentation culture of bacillus polymyxa, destruxinus destructor, bacillus thuringiensis and streptomycete is carried out, bacillus polymyxa, destruxinus, bacillus thuringiensis and streptomycete are obtained respectively, then the obtained bacillus polymyxa, destruxinus, bacillus thuringiensis and streptomycete are respectively subjected to rotary culture, the rotating speed is 350r/min, the rotation is carried out for 20 minutes, then each bacterium liquid is taken out, live bacteria and dead bacteria in each bacterium are separated by trypan blue, and the live bacteria are taken out;

(2) weighing 40g of bacillus polymyxa liquid, 30g of metarhizium anisopliae liquid, 30g of bacillus thuringiensis liquid and 20g of streptomycete liquid, respectively carrying out spray drying on the liquid to prepare powdery bacterium powder, then placing the bacterium powder together for mixing, putting the mixed bacterium powder into the carrier liquid prepared in the step (1), stirring and mixing, adding 30g of protective agent after uniformly stirring, wherein the protective agent is a composition among hydrolyzed collagen, chitin, cyclodextrin and gelatin, and the mass ratio among the hydrolyzed collagen, the chitin, the cyclodextrin and the gelatin is 1.6: 1: 1.7: 1; after being stirred uniformly, 90g of bentonite is added, and the product can be obtained after being stirred uniformly, wherein the number of effective viable bacteria in the product is 2 to 3 hundred million/g.

Example 12

A preparation method of a compound microbial agent for preventing insect pests specifically comprises the following steps:

(1) crushing 70g of carrier, namely diatomite, sieving the carrier by a 150-mesh sieve, and preparing slurry with the carrier powder at a mass percent of 75%; liquid fermentation culture of bacillus polymyxa, destruxinus destructor, bacillus thuringiensis and streptomycete is carried out, bacillus polymyxa, destruxinus, bacillus thuringiensis and streptomycete are obtained respectively, then the obtained bacillus polymyxa, destruxinus, bacillus thuringiensis and streptomycete are respectively subjected to rotary culture, the rotating speed is 400r/min, the rotation is carried out for 20 minutes, then each bacterium liquid is taken out, live bacteria and dead bacteria in each bacterium are separated by trypan blue, and the live bacteria are taken out;

(2) weighing 40g of bacillus polymyxa liquid, 30g of metarhizium anisopliae liquid, 30g of bacillus thuringiensis liquid and 20g of streptomycete liquid, respectively carrying out spray drying on the liquid to prepare powdery bacterium powder, then placing the bacterium powder together for mixing, putting the mixed bacterium powder into the carrier liquid prepared in the step (1), stirring and mixing, adding 30g of protective agent after uniformly stirring, wherein the protective agent is a composition among hydrolyzed collagen, chitin, cyclodextrin and gelatin, and the mass ratio among the hydrolyzed collagen, the chitin, the cyclodextrin and the gelatin is 1.6: 1: 1.7: 1; after being stirred uniformly, 90g of bentonite is added, and the product can be obtained after being stirred uniformly, wherein the number of effective viable bacteria in the product is 2 to 3 hundred million/g.

Comparative example 1

A preparation method of a compound microbial agent for preventing insect pests specifically comprises the following steps:

(1) crushing 70g of carrier, namely diatomite, sieving the carrier by a 150-mesh sieve, and preparing slurry with the carrier powder at a mass percent of 75%; liquid fermentation culture of bacillus polymyxa, metarhizium anisopliae and streptomycete is carried out, bacillus polymyxa, metarhizium anisopliae and streptomycete are obtained respectively, then the obtained bacillus polymyxa, metarhizium anisopliae and streptomycete are respectively subjected to rotary culture, the rotating speed is 350r/min, the bacteria are rotated for 18 minutes, then the bacteria are taken out, viable bacteria and dead bacteria in the bacteria are separated by trypan blue, and the viable bacteria are taken out;

(2) weighing 40g of bacillus polymyxa bacterial liquid, 30g of metarhizium anisopliae bacterial liquid and 20g of streptomycete bacterial liquid, respectively carrying out spray drying on the bacterial liquids to prepare powdery bacterial powder, then placing the bacterial powders together for mixing, putting the mixed bacterial powder into the carrier liquid prepared in the step (1), stirring and mixing, adding 30g of protective agent after uniformly stirring, wherein the protective agent is a composition among hydrolyzed collagen, chitin, cyclodextrin and gelatin, and the mass ratio among the hydrolyzed collagen, the chitin, the cyclodextrin and the gelatin is 1.6: 1: 1.7: 1; after being stirred uniformly, 90g of bentonite is added, and the product can be obtained after being stirred uniformly, wherein the number of effective viable bacteria in the product is 2 to 3 hundred million/g.

Comparative example 2

A preparation method of a compound microbial agent for preventing insect pests specifically comprises the following steps:

(1) crushing 70g of carrier, namely diatomite, sieving the carrier by a 150-mesh sieve, and preparing slurry with the carrier powder at a mass percent of 75%; liquid fermentation culture of bacillus polymyxa, bacillus thuringiensis and streptomycete is carried out, bacillus polymyxa, bacillus thuringiensis and streptomycete are obtained respectively, then the obtained bacillus polymyxa, bacillus thuringiensis and streptomycete are subjected to rotary culture respectively, the rotating speed is 350r/min, the rotation is 18 minutes, then all bacteria are taken out, live bacteria and dead bacteria in strains are separated by trypan blue, and the live bacteria are taken out;

(2) weighing 40g of bacillus polymyxa liquid, 30g of bacillus thuringiensis liquid and 20g of streptomycete liquid, respectively carrying out spray drying on the liquid to prepare powdery bacterial powder, then placing the bacterial powders together for mixing, putting the mixed bacterial powder into the carrier slurry prepared in the step (1), stirring and mixing, adding 30g of protective agent after uniformly stirring, wherein the protective agent is a composition among hydrolyzed collagen, chitin, cyclodextrin and gelatin, and the mass ratio among the hydrolyzed collagen, the chitin, the cyclodextrin and the gelatin is 1.6: 1: 1.7: 1; after being stirred uniformly, 90g of bentonite is added, and the product can be obtained after being stirred uniformly, wherein the number of effective viable bacteria in the product is 2 to 3 hundred million/g.

Comparative example 3

A preparation method of a compound microbial agent for preventing insect pests specifically comprises the following steps:

(1) crushing 70g of carrier, namely diatomite, sieving the carrier by a 150-mesh sieve, and preparing slurry with the carrier powder at a mass percent of 75%; liquid fermentation culture of bacillus polymyxa and streptomycete is carried out, bacillus polymyxa bacteria liquid and streptomycete bacteria liquid are respectively obtained, then the obtained bacillus polymyxa bacteria liquid and streptomycete bacteria liquid are respectively subjected to rotary culture, the rotating speed is 350r/min, the bacteria liquid is rotated for 18 minutes, then all bacteria liquid is taken out, viable bacteria and dead bacteria in strains are separated by trypan blue, and the viable bacteria are taken out;

(2) weighing 40g of bacillus polymyxa bacterial liquid and 20g of streptomycete bacterial liquid, respectively carrying out spray drying on each bacterial liquid to prepare powdery bacterial powder, then placing each bacterial powder together for mixing, putting the mixed bacterial powder into the carrier liquid prepared in the step (1), stirring and mixing, adding 30g of protective agent after uniformly stirring, wherein the protective agent is a composition among hydrolyzed collagen, chitin, cyclodextrin and gelatin, and the mass ratio among the hydrolyzed collagen, the chitin, the cyclodextrin and the gelatin is 1.6: 1: 1.7: 1; after being stirred uniformly, 90g of bentonite is added, and the product can be obtained after being stirred uniformly, wherein the number of effective viable bacteria in the product is 2 to 3 hundred million/g.

Selecting 15 groups of experimental lands with cutworms, digging holes when corns are planted, respectively putting the compound microbial agents prepared in the examples 1-12 and the comparative examples 1-3 into the holes of the corresponding groups according to the dosage of 3 kg/mu, selecting healthy corn seedlings to be put into the holes, watering for planting, using 4 kg/mu in the middle and later growth periods, and observing the control effect on the cutworms.

TABLE 1

In summary, the following steps: as can be seen from Table 1, the embodiment of the invention has an effective killing effect on cutworms, can prevent the cutworms from appearing repeatedly and is beneficial to plant growth, and the invention provides a compound microbial agent for preventing insect pests and an application thereof.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.