阅读说明：本技术 玛咖提取物在制备药物或食品中的用途 (Use of maca extract in preparation of medicine or food ) 是由 刘俊彦 汉默克·布鲁斯·杜普瑞 巴尼奇·博格丹 黄宋熙 莫里索·克里斯托弗 杨军 于 2020-06-08 设计创作，主要内容包括：本发明公开了一种玛咖提取物在制备药物或食品中的用途。本发明用玛咖提取玛咖酰胺,发现被提取出来的玛咖酰胺是一类水溶性环氧化物水解酶抑制剂,所述抑制剂有抗炎、止痛、降血压、降血糖、保护心血管、肾脏和神经系统等多种功能,特别是其中的N-苄基亚油酰胺具备良好的抗炎止痛功效。本发明解决了目前水溶性环氧化物水解酶抑制剂人工合成难、成本高等诸多问题。将上述玛咖提取物制备成抗炎止痛、降血压、降血糖、保护心血管、肾脏和神经系统的药品或食品,具有广阔的市场前景。(The invention discloses application of a maca extract in preparation of medicines or foods. According to the invention, macamide is extracted from maca, the extracted macamide is found to be a water-soluble epoxide hydrolase inhibitor, the inhibitor has multiple functions of resisting inflammation, relieving pain, reducing blood pressure and blood sugar, protecting cardiovascular, kidney and nervous systems and the like, and particularly the N-benzyl linoleamide has good anti-inflammatory and analgesic effects. The invention solves the problems of difficult artificial synthesis, high cost and the like of the existing water-soluble epoxide hydrolase inhibitor. The maca extract is prepared into medicines or foods with the effects of resisting inflammation, relieving pain, reducing blood pressure and blood sugar, and protecting cardiovascular system, kidney and nervous system, and has wide market prospect.)

1. Use of a maca extract in the preparation of a medicament or food product, characterised in that the extract is macamide.

2. The use according to claim 1, wherein the macamide is a water-soluble epoxide hydrolase inhibitor.

3. Use according to claim 1 or 2, characterized in that the macamide is:

n-benzyl linoleamide;

n-benzyl linolenamide;

n-benzyl arachidonamide;

n-benzyl eicosa-5Z, 8Z,11Z,14Z, 17Z-pentaenamide;

n-3-methoxybenzyl linoleamide;

n-3-methoxybenzyl linolenamide;

n-3-methoxybenzyl arachidonamide;

n-3-methoxybenzyl eicosa-5Z, 8Z,11Z,14Z, 17Z-pentaenamide;

n-3, 4-dimethoxybenzyl linolenamide;

n- (2-phenylethyl) linoleamide;

n- (2-phenylethyl) linolenamide;

any one or a combination of any two or more.

4. Use according to claim 1 or 2, wherein the medicament or food product is an analgesic and or anti-inflammatory product.

5. Use according to claim 3, wherein the medicament or food product is an analgesic and or anti-inflammatory product.

6. Use according to claim 1 or 2, wherein the function of the medicament or food product is one or a combination of any of the following:

protecting the cardiovascular system; the kidney is protected; protecting the nervous system.

7. Use according to claim 3, wherein the function of the medicament or food product is one or a combination of any of:

protecting the cardiovascular system; the kidney is protected; protecting the nervous system.

Technical Field

The invention relates to the technical field of edible and medicinal application of maca, in particular to the technical field of edible and medicinal application of a maca extract.

Background

Lepidium meyenii belongs to annual or biennial herb of Lepidium of Brassicaceae, and is native to 3500m-4500m south America Andes mountain area of Peruvian sea. Maca is called "Peru ginseng" in south America because of its efficacy comparable to that of ginseng, and is a plant used as both medicine and food. The root of the underground expanded and juicy maca reservoir is radish-like, and is the main edible part with commercial value. In the past 20 years, maca has become more and more well understood and of increasing concern around the world. The natural conditions of western regions in China are suitable for the growth of maca, introduction of seeds is realized in provinces (regions) such as Yunnan, Xinjiang, Jilin and Tibet in China, and the economic and social benefits are good. Researches show that maca has the effects of improving the fertility of people and animals, improving sexual functions, inhibiting enlargement of prostate, resisting fatigue and depression, improving osteoporosis, improving memory and the like.

However, water-soluble antioxidant hydrolase inhibitors have never been reported in the literature in maca extracts. Water-soluble epoxide hydrolase (sEH) is an enzyme capable of metabolizing epoxyfatty acids, which is widely present in mammals, converts endogenous epoxyeicosatrienoic acids (EETs) to dihydroxyeicosatrienoic acids (DHETs). Endogenous EETs are obtained by oxidizing Arachidonic Acid (AA) through certain cytochrome P450 monooxygenases, are important signal molecules in organisms, and have the functions of regulating ion transport, gene expression, vasodilatation and the like. In animals, there are many ways to degrade the EETs, and the sEH metabolizes the EETs into DHETs as the main metabolic pathway, so that the EETs are reduced in concentration and reduced in physiological activity, which may lead to an increase in blood pressure and further affect the functions of kidney and heart. Directly functionally, the water-soluble redox hydrolase inhibitor has various efficacies of reducing blood pressure and blood sugar, protecting the cardiovascular system, the kidney system, the nervous system and the like, and currently, clinical experiments and application of sEH are carried out by a plurality of companies in the United states, but the sEH is a small molecular compound synthesized in a laboratory, and the high-efficiency water-soluble redox hydrolase inhibitor from a natural source is very rare.

Therefore, those skilled in the art are working on developing a highly effective water-soluble epoxide hydrolase inhibitor from natural sources, and related drugs and related functional foods with water-soluble epoxide hydrolase as an action target.

Disclosure of Invention

In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is to obtain a highly effective water-soluble redox hydrolase inhibitor of natural origin, and develop a series of medicines and functional foods around the efficacy of the inhibitor, so as to overcome the problems of high cost, small quantity and small molecule of the artificial synthesis of the water-soluble redox hydrolase inhibitor.

In order to achieve the above object, the present invention provides a use of a maca extract, which is macamide, in the preparation of a medicament or food.

Further, the macamide is a water-soluble epoxide hydrolase inhibitor.

Further, the macamides are

N-benzyl linoleamide;

n-benzyl linolenamide;

n-benzyl arachidonamide;

n-benzyl eicosa-5Z, 8Z,11Z,14Z, 17Z-pentaenamide;

n-3-methoxybenzyl linoleamide;

n-3-methoxybenzyl linolenamide;

n-3-methoxybenzyl arachidonamide;

n-3-methoxybenzyl eicosa-5Z, 8Z,11Z,14Z, 17Z-pentaenamide;

n-3, 4-dimethoxybenzyl linolenamide;

n- (2-phenylethyl) linoleamide;

n- (2-phenylethyl) linolenamide;

any one or a combination of any two or more.

Further, the medicament or food product is an analgesic and or anti-inflammatory product.

Further, the medicament or food product is an analgesic and or anti-inflammatory product.

Further, the function of the drug or food is one or a combination of any of:

protecting the cardiovascular system; the kidney is protected; protecting the nervous system.

Further, the function of the drug or food is one or a combination of any of:

protecting the cardiovascular system; the kidney is protected; protecting the nervous system.

Technical effects

The project firstly clarifies that a class of compounds in maca extract maca amide have the activity of inhibiting water-soluble epoxide hydrolase, proves that the compounds have the function of relieving pain, and can develop a series of medicines, natural medicines and foods taking the inhibiting water-soluble epoxide hydrolase as a target point.

The conception, the specific structure and the technical effects of the present invention will be further described with reference to the accompanying drawings to fully understand the objects, the features and the effects of the present invention.

Drawings

FIG. 1 is a compound structural diagram of N-benzylpalmitamide;

FIG. 2 is a compound structural diagram of N-benzylstearamide;

FIG. 3 is a compound structural diagram of N-benzyl oleamide;

FIG. 4 is a structural diagram of a compound of N-benzyl linoleamide

FIG. 5 is a compound structural diagram of N-benzyl linolenamide

FIG. 6 is a compound structural diagram of N-benzyl arachidonamide

FIG. 7 is a compound structural diagram of N-benzyl eicosa-5Z, 8Z,11Z,14Z, 17Z-pentaenamide

FIG. 8 is a structural diagram of a compound of N-3-methoxybenzyl palmitamide

FIG. 9 is a compound structural diagram of N-3-methoxybenzyl stearamide

FIG. 10 is a compound structural diagram of N-3-methoxybenzyl oleamide

FIG. 11 is a compound structural diagram of N-3-methoxybenzyl linoleamide

FIG. 12 is a compound structural diagram of N-3-methoxybenzyl linolenamide

FIG. 13 is a compound structural diagram of N-3-methoxybenzyl arachidonamide

FIG. 14 is a compound structural diagram of N-3-methoxybenzyl eicosa-5Z, 8Z,11Z,14Z, 17Z-pentaenamide

FIG. 15 is a structural diagram of a compound of N-3, 4-dimethoxybenzylpalmitamide

FIG. 16 is a structural diagram of a compound of N-3, 4-dimethoxybenzyl linoleamide

FIG. 17 is a compound structural diagram of N-3, 4-dimethoxybenzyl linolenamide

FIG. 18 is a structural diagram of a compound of N- (2-phenylethyl) linoleamide

FIG. 19 is a compound structural diagram of N- (2-phenylethyl) linolenamide

FIG. 20 is a graph of experimental data demonstrating the anti-inflammatory analgesic effect of macamides

Detailed Description

Definitions of terms of art:

①IC₅₀(half maximum inhibition concentration) refers to the half inhibitory concentration of the antagonist being measured. It indicates that a drug or substance (inhibitor) is inhibiting half the amount of a biological process (or a substance, such as an enzyme, cellular receptor or microorganism, that is involved in the process). IC (integrated circuit)₅₀The value can be used to measure the ability of a drug to inhibit an enzyme, i.e., the stronger the inhibitory ability, the lower the value.

The CAS Number (CAS Registry Number or CAS Number, CAS Rn, CAS #), also called CAS accession Number, is a unique Number identification Number of a certain substance (compound, polymer material, Biological sequence, mixture or alloy). The lower histochemical Abstract Service (CAS) of the American Chemical society is responsible for assigning a CAS number to each substance appearing in the literature, in order to avoid the trouble of multiple names for chemicals and to facilitate database retrieval. Almost all chemical databases today allow searching with CAS numbers.

③ nM represents units of nmol/L, read as: nanomole per liter;

n is "na", a common unit being nM, 1M ═ 10^ s⁹nM. M, i.e. "mol/L", is read as: moles per liter.

sEH refers to a water-soluble epoxide hydrolase, water-soluble epoxide hydrolase (sEH).

Example 1

The maca can be extractedA macamide is obtained, the macamide is a water-soluble epoxide hydrolase inhibitor, specifically is N-benzyl palmitamide, a compound structure diagram of the N-benzyl palmitamide is shown in figure 1, and the CAS number of the N-benzyl palmitamide is 74058-71-2. The inventors tested the inhibitory effect of N-benzyl palmitamide on human, rat and mouse water-soluble epoxide hydrolase, where IC is of human sEH₅₀IC of sEH in rat with (nM) of 524₅₀IC of sEH for mouse with (nM) of 370₅₀(nM) is 422. The N-benzyl palmitamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 2

Maca can be extracted to obtain maca amide which is a water-soluble epoxide hydrolase inhibitor, specifically N-benzyl stearamide, the structure of the compound of the N-benzyl stearamide is shown in figure 2, and the CAS number of the N-benzyl stearamide is 5327-45-7. The inventors tested the inhibitory effect of N-benzylstearamide on human, rat and mouse water-soluble epoxide hydrolase, where IC is of human sEH₅₀(nM) IC of sEH over 100000 for rats₅₀IC of sEH for mouse with (nM) of 35755₅₀(nM) is 59564. The N-benzyl stearamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 3

Maca extracts a macamide which is a water-soluble epoxide hydrolase inhibitor, in particular N-benzyl oleamide, the structure of which is shown in FIG. 3, with CAS number 101762-87-2. The inventors tested the inhibitory effect of N-benzyl oleamide on human, rat and mouse water-soluble epoxide hydrolase, where IC is of human sEH₅₀IC of sEH for rat with (nM) 893₅₀IC of sEH for mouse with (nM) of 198₅₀(nM) is 265. The N-benzyl oleamide may beThe preparation method is used for preparing a medicine or food, and the medicine or the food has one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 4

Maca extracts a macamide which is a water-soluble epoxide hydrolase inhibitor, specifically N-benzyl linoleamide, the structure of which is shown in FIG. 4, and the CAS number of N-benzyl linoleamide is 18286-71-0. The inventors tested the inhibitory effect of N-benzyl linoleamide on human, rat and mouse water-soluble epoxide hydrolases, where IC is of human sEH₅₀IC of sEH in rat with (nM) of 155₅₀IC of sEH for mouse with (nM) of 41₅₀(nM) is 44. The N-benzyl linoleamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

The pharmacokinetic parameters of N-benzyl linoleamide in the rat model are as follows:

the IC of rats orally administered N-benzyl linoleamide (dose 100mg/kg rat body weight) reached a peak concentration of 520nM (Cmax 520nM) in blood after 3 hours (Tmax 3h) of 3 hours₅₀11.8 times of the total amount of the compound, and the half-life period is 9 hours (t1/2 is 9 h). The present inventors studied the anti-inflammatory analgesic effect of N-benzyl linoleamide having strong sEH inhibitory effect using LPS-induced inflammatory pain model in rats, and the results are shown in fig. 20, where N-benzyl linoleamide (dose 100mg/kg body weight of rats) orally administered has significant anti-inflammatory analgesic effect.

LPS induced inflammatory pain in the rat paw, as shown in FIG. 20, the rat paw was more sensitive to pain stimulation and the tolerance to pain decreased to around 60% of that before LPS treatment (black filled circles), while oral administration of N-benzyl linoleamide (dose 100mg/kg rat body weight) reduced the LPS induced inflammatory pain in the rat, as compared to the LPS treated model group, the drug group (grey filled triangles) had higher tolerance to pain than the LPS treated group, indicating that N-benzyl linoleamide had anti-inflammatory and analgesic effects. Data are mean ± standard error (N ═ 4), and the tolerance to pain for LPS and pre-drug treated rats was defined as 100%.

Example 5

Maca extracts a macamides which is a water-soluble epoxide hydrolase inhibitor, in particular N-benzyl linolenamide, the structure of which is shown in FIG. 5, with CAS number 883715-18-2. The inventors tested the inhibitory effect of N-benzyl linolenamide on human, rat and mouse water-soluble epoxide hydrolases, where IC is of human sEH₅₀IC of sEH for rat with (nM) of 134₅₀IC of sEH for mouse with (nM) of 23₅₀(nM) is 28. The N-benzyl linolenamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 6

Maca can be extracted to obtain maca amide which is a water-soluble epoxide hydrolase inhibitor, specifically N-benzyl arachidonamide, and FIG. 6 shows the structure diagram of the compound of the N-benzyl arachidonamide. The inventors tested the inhibitory effect of N-benzyl arachidonamide on human, rat and mouse water-soluble epoxide hydrolase, where IC is of human sEH₅₀IC of sEH for rat with (nM) 123₅₀IC of sEH for mice with (nM) of 40₅₀(nM) is 50. The N-benzyl peanut tetraenamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 7

Maca can be extracted to obtain maca amide which is a water-soluble epoxide hydrolase inhibitor, specifically N-benzyl eicosa-5Z, 8Z,11Z,14Z, 17Z-pentaenamide, and the structure diagram of the compound of the N-benzyl eicosa-5Z, 8Z,11Z,14Z, 17Z-pentaenamide is shown in figure 7. The inventor tests N-benzyl eicosa-5Z, 8Z,11Z,14Z, 17Z-pentaenamide on human and largeInhibition of murine and murine Water-soluble epoxide Hydrolase, where IC is of human sEH₅₀IC of sEH for rat with (nM) of 111₅₀IC of sEH for mouse with (nM) of 29₅₀(nM) is 43. The N-benzyl eicosa-5Z, 8Z,11Z,14Z, 17Z-pentaenamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 8

Maca can be used for extracting maca amide which is a water-soluble epoxide hydrolase inhibitor, specifically N-3-methoxybenzyl palmitamide, and figure 8 shows the structure diagram of the compound of the N-3-methoxybenzyl palmitamide, and the CAS number of the N-3-methoxybenzyl palmitamide is 5327-45-7. The inventors tested the inhibitory effect of N-3-methoxybenzyl palmitamide on human, rat and mouse water-soluble epoxide hydrolases, where IC is on human sEH₅₀IC of sEH in rats with (nM) 16077₅₀IC of sEH for mouse with (nM) of 28676₅₀(nM) is 89850. The N-3-methoxybenzyl palmitamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 9

Maca can be extracted to obtain maca amide which is a water-soluble epoxide hydrolase inhibitor, specifically N-3-methoxybenzyl stearamide, and FIG. 9 shows the structure of the compound of the N-3-methoxybenzyl stearamide, wherein the CAS number of the N-3-methoxybenzyl stearamide is 883715-21-7. The inventors tested the inhibitory effect of N-3-methoxybenzyl stearamide on human, rat and mouse water soluble epoxide hydrolases, where the IC50(nM) for human sEH was 241 and for rat sEH₅₀IC of sEH for mouse with (nM) of 84₅₀(nM) is 126. The N-3-methoxybenzyl stearamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting skinProtecting the nervous system.

Example 10

Maca can be extracted to obtain maca amide which is a water-soluble epoxide hydrolase inhibitor, specifically N-3-methoxybenzyl oleamide, the structure diagram of the compound of the N-3-methoxybenzyl oleamide is shown in figure 10, and the CAS number of the N-3-methoxybenzyl oleamide is 74058-71-2. The inventors tested the inhibitory effect of N-3-methoxybenzyl oleamide on human, rat and mouse water-soluble epoxide hydrolases, where IC is on human sEH₅₀IC of sEH in rat with (nM) of 241₅₀IC of sEH for mouse with (nM) of 84₅₀(nM) is 126. The N-3-methoxybenzyl oleamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 11

Maca can be extracted to obtain maca amide which is a water-soluble epoxide hydrolase inhibitor, specifically N-3-methoxybenzyl linoleamide, and FIG. 11 shows the structure of the compound of the N-3-methoxybenzyl linoleamide. The inventors tested the inhibitory effect of N-3-methoxybenzyl linoleamide on human, rat and mouse water-soluble epoxide hydrolases, where IC is on human sEH₅₀IC of sEH for rat with (nM) 92₅₀IC of sEH for mouse with (nM) of 43₅₀(nM) is 38. The N-3-methoxybenzyl linoleamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 12

Maca can be extracted to obtain maca amide which is a water-soluble epoxide hydrolase inhibitor, specifically N-3-methoxybenzyl linolenamide, and a compound structure diagram of the N-3-methoxybenzyl linolenamide is shown in figure 12. The inventors tested the inhibitory effect of N-3-methoxybenzyl linolenamide on human, rat and mouse water-soluble epoxide hydrolases, where the sE is humanIC of H₅₀IC of sEH for rat with (nM) of 76₅₀IC of sEH for mouse with (nM) of 9.3₅₀(nM) was 27. The N-3-methoxybenzyl linolenamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 13

Maca can be extracted to obtain maca amide which is a water-soluble epoxide hydrolase inhibitor, specifically N-3-methoxybenzyl arachidonamide, and FIG. 13 shows the structure of the compound of the N-3-methoxybenzyl arachidonamide. The inventors tested the inhibitory effect of N-3-methoxybenzyl arachidonamide on human, rat and mouse water-soluble epoxide hydrolases, where IC is on human sEH₅₀IC of sEH in rat with (nM) of 55₅₀IC of sEH for mice with (nM) of 22₅₀(nM) is 73. The N-3-methoxybenzyl arachidonamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 14

Maca can be extracted to obtain maca amide which is a water-soluble epoxide hydrolase inhibitor, specifically N-3-methoxybenzyl eicosa-5Z, 8Z,11Z,14Z, 17Z-pentaenamide, and a compound structure diagram of the N-3-methoxybenzyl eicosa-5Z, 8Z,11Z,14Z, 17Z-pentaenamide is shown in figure 14. The inventors tested the inhibitory effect of N-3-methoxybenzyl eicosa-5Z, 8Z,11Z,14Z, 17Z-pentaenamide on human, rat and mouse water-soluble epoxide hydrolases, where IC is on human sEH₅₀IC of sEH for rat with (nM) 63₅₀(nM) 18.6, IC of sEH for mice₅₀(nM) is 49. The N-3-methoxybenzyl eicosane-5Z, 8Z,11Z,14Z, 17Z-pentaenamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 15

Maca can be extracted to obtain maca amide which is a water-soluble epoxide hydrolase inhibitor, specifically N-3, 4-dimethoxybenzyl palmitamide, the structure diagram of the compound of the N-3, 4-dimethoxybenzyl palmitamide is shown in figure 15, and the CAS number of the N-3, 4-dimethoxybenzyl palmitamide is 74058-71-2. The inventors tested the inhibitory effect of N-3, 4-dimethoxybenzyl-palmitamide on human, rat and mouse water-soluble epoxide hydrolases, where IC is on human sEH₅₀(nM) 7059, IC of sEH in rat₅₀IC of sEH for mouse with (nM) of 15910₅₀(nM) is greater than 100000. The N-3, 4-dimethoxy benzyl palmitamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 16

Maca can be extracted to obtain maca amide which is a water-soluble epoxide hydrolase inhibitor, specifically N-3, 4-dimethoxybenzyl linoleamide, the compound structure diagram of the N-3, 4-dimethoxybenzyl linoleamide is shown in figure 16, and the CAS number of the N-3, 4-dimethoxybenzyl linoleamide is 74058-71-2. The inventors tested the inhibitory effect of N-3, 4-dimethoxybenzyl linoleamide on human, rat and mouse water-soluble epoxide hydrolases, where IC is on human sEH₅₀IC of sEH in rats with (nM) of 104₅₀IC of sEH for mouse with (nM) of 111₅₀(nM) is 148. The N-3, 4-dimethoxy benzyl linoleamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 17

Maca can be extracted to obtain maca amide which is a water-soluble epoxide hydrolase inhibitor, specifically N-3, 4-dimethoxybenzyl linolenamide, and a compound structure diagram of the N-3, 4-dimethoxybenzyl linolenamide is shown in figure 17. The inventors tested N-3, 4-dimethoxybenzylInhibition of human, rat and mouse water-soluble epoxide hydrolases by linolenamides, in which IC is of human sEH₅₀IC of sEH in rat with (nM) of 84₅₀IC of sEH for mouse with (nM) 90₅₀(nM) is 122. The N-3, 4-dimethoxy benzyl linolenamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 18

Maca extracts a macamide which is a water-soluble epoxide hydrolase inhibitor, specifically N- (2-phenylethyl) linoleamide, and fig. 18 shows the structure of the compound of N- (2-phenylethyl) linoleamide. The inventors tested the inhibitory effect of N- (2-phenylethyl) linoleamide on water-soluble epoxide hydrolase of human, rat and mouse, wherein IC of sEH of human₅₀IC of sEH for rat with (nM) of 28₅₀IC of sEH for mouse with (nM) of 33₅₀(nM) was 91. The N- (2-phenylethyl) linoleamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 19

Maca extracts a macamide which is a water-soluble epoxide hydrolase inhibitor, specifically N- (2-phenylethyl) linolenamide, and fig. 19 shows the structure of the compound of N- (2-phenylethyl) linolenamide, which has CAS number 74058-71-2. The inventors tested the inhibitory effect of N- (2-phenylethyl) linolenamide on human, rat and mouse water-soluble epoxide hydrolases, where IC was on human sEH₅₀IC of sEH in rat with (nM) of 524₅₀IC of sEH for mouse with (nM) of 370₅₀(nM) is 422. The N- (2-phenethyl) linolenamide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 20

Maca can be extracted to obtain maca amide, and the maca amide can be used for preparing medicines or foods, and the medicines or the foods have one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

Example 21

Maca can extract a water-soluble epoxide hydrolase inhibitor, which can be used for preparing a drug or a food, wherein the drug or the food has one or more of the following functions: relieving pain, relieving inflammation, protecting cardiovascular system, protecting kidney, and protecting nervous system.

The foregoing detailed description of the preferred embodiments of the invention has been presented. It should be understood that numerous modifications and variations could be devised by those skilled in the art in light of the present teachings without departing from the inventive concepts. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning and limited experiments based on the prior art according to the concept of the present invention should be within the scope of protection defined by the claims.