阅读说明：本技术 一种高纯度安丝菌素的制备方法 (Preparation method of high-purity ansamitocin ) 是由 肖红亮 刘吉东 郭明 袁建栋 于 2020-03-25 设计创作，主要内容包括：本发明属于工业微生物技术领域,具体涉及一种高纯度安丝菌素的制备方法。本发明的高纯度安丝菌素的制备方法,包括以下步骤：对安丝菌素发酵液进行预处理,之后加入助滤剂压滤得到菌丝渣；用极性溶剂对菌丝渣进行浸提,过滤,得到浸提液；浓缩除去浸提液中的溶剂,得浓缩液,然后加入不相溶于水的萃取剂进行萃取；将萃取出的有机相依次进行浓缩、降温结晶,得到安丝菌素粗品A；将粗品A用混合溶剂结晶得到粗品B,所述混合溶剂为乙酸乙酯、二氯甲烷中的任意一种与正己烷、正庚烷中的任意一种的组合；用极性混合溶剂对粗品B进行结晶,得到晶体C,干燥即得高纯度安丝菌素成品。本发明方法得到的安丝菌素纯度达到92％以上。(The invention belongs to the technical field of industrial microorganisms, and particularly relates to a preparation method of high-purity ansamitocin. The preparation method of the high-purity ansamitocin comprises the following steps: pretreating ansamitocin fermentation liquor, and then adding a filter aid for filter pressing to obtain hypha residues; leaching the mycelium residues with a polar solvent, and filtering to obtain a leaching solution; concentrating to remove solvent in the leaching solution to obtain concentrated solution, and then adding an extractant which is not dissolved in water for extraction; concentrating the extracted organic phase, cooling and crystallizing to obtain crude ansamitocin A; crystallizing the crude product A with a mixed solvent to obtain a crude product B, wherein the mixed solvent is a combination of any one of ethyl acetate and dichloromethane and any one of n-hexane and n-heptane; and crystallizing the crude product B by using a polar mixed solvent to obtain a crystal C, and drying to obtain a high-purity ansamitocin finished product. The purity of the ansamitocins obtained by the method provided by the invention reaches more than 92%.)

1. A preparation method of high-purity ansamitocin is characterized by comprising the following steps:

1) pretreating ansamitocin fermentation liquor, and then adding a filter aid for filter pressing to obtain hypha residues;

2) leaching the mycelium residues in the step 1) by using a polar solvent, and filtering to obtain a leaching solution;

3) concentrating to remove the solvent in the leaching liquor in the step 2) to obtain a concentrated solution, and then adding an extractant which is not dissolved in water into the concentrated solution for extraction;

4) concentrating the extracted organic phase, cooling and crystallizing to obtain crude ansamitocin A;

5) crystallizing the crude product A in the step 4) by using a mixed solvent to obtain a crude product B, wherein the mixed solvent is a combination of any one of ethyl acetate and dichloromethane and any one of n-hexane and n-heptane;

6) crystallizing the crude product B by using a polar mixed solvent to obtain a crystal C, and drying to obtain a high-purity ansamitocin finished product; the polar mixed solvent is the combination of any one of methanol, ethanol and acetone and water.

2. The method according to claim 1, wherein the pretreatment in step 1) is to adjust the pH of the ansamitocin fermentation broth to 2.0 to 5.0.

3. The method according to claim 1, wherein the filter aid in step 1) is perlite or diatomaceous earth.

4. The preparation method according to claim 1, characterized in that 20-60 g of filter aid is added per liter of ansamitocin fermentation broth in step 1).

5. The preparation method according to claim 1, wherein the polar solvent in step 2) is one or more of methanol, ethanol, acetone and ethyl acetate, and 2-5L of the polar solvent is used for extraction per kilogram of hypha residues.

6. The preparation method of claim 1, wherein the leaching time in the step 2) is 6-15 h.

7. The preparation method of claim 1, wherein the water-immiscible extractant in step 3) is ethyl acetate, butyl acetate, dichloromethane or toluene, the addition amount of the extractant is 0.5-2 times of the volume of the concentrated solution, and the extraction time is 1-5 h.

8. The preparation method according to claim 1, wherein the temperature in the step 4) is reduced to 0-30 ℃ for crystallization.

9. The production method according to any one of claims 1 to 8, wherein the drying in step 6) is drying under reduced pressure.

10. The preparation method according to claim 9, wherein the temperature of the reduced pressure drying is 30-60 ℃ and the time is 8-16 h.

Technical Field

The invention belongs to the technical field of industrial microorganisms, and particularly relates to a preparation method of high-purity ansamitocin.

Background

Ansamitocin (structure shown in formula 1) is a Maytansinoid (Maytansinoid) antibiotic, is mainly obtained by fermentation production of microorganisms such as Actinosynnema leucotrichum (Actinosynnema depressa TCC31565) or separation from higher plants, and has various pharmacological activities such as anti-tumor activity, anti-tubercle bacillus activity, anti-bacterial activity and the like.

Higsahide et al, in 1977, isolated a novel ansamycin with potential anticancer activity from Nocardia C-15003 fermentation broth, with its components linked to carbon chains of different lengths according to C-3, which was classified into P-0, P-1, P-2, P-3', P-4 and P-4', where the ansamitocin P-3(AP-3) linked to isobutyl group is most active and also produces higher yields in all fermentatively produced ansamitocins, so ansamitocin P-3 is often the target product of fermentation, which can prevent cell mitosis by hindering microtubule formation to cause cell death, and has significant antitumor activity in vitro and in tumor-bearing animals.

Ansamitocin P-3 is a chemical precursor of the antineoplastic drug DM 1. DM1 is a chemical small molecule synthesized by chemical modification of a chemotherapeutics called maytansinol, DM1 can be coupled with a targeting antibody through a linker to prepare a targeting antitumor drug, so that the side effect of chemotherapy is reduced, and the curative effect is enhanced. New drug T-DM1(trastuzumab-DM 1) for treating breast cancer, namely a coupling drug of antibody drug Herceptin-DM 1, of Roche company is used for delaying the growth and development time of tumors by 3.2 months compared with lapatinib in patients with advanced breast cancer.

At present, most of ansamitocins are reported in fermentation processes, and few of ansamitocins are also vague in methods or too complicated in processes, are biased to small-amount sample preparation and are not suitable for large-scale production. Methods such as described in CN102731526A, CN102732581A and US7432088 require the use of normal phase silica gel chromatography or medium pressure reverse phase silica gel chromatography; however, the extraction with the whole fermentation liquor in US6573074B2 and US20020015984 consumes too much solvent, and the fermentation liquor needs to be heated to 75 ℃ before extraction, which results in the pollution of the environment and the increase of the operation risk due to the volatilization of a large amount of extraction solvent.

Therefore, there is an urgent need in the art to find a purification method that improves the yield and purity of the product, reduces the production cost, has simple operation steps, and is environmentally friendly, so as to overcome the above-mentioned defects in the prior art and meet the demand of industrial production.

Disclosure of Invention

The invention aims to provide a preparation method of high-purity ansamitocin, and the ansamitocin obtained by the method has high purity and yield.

In order to achieve the purpose, the technical scheme of the invention is as follows:

a preparation method of high-purity ansamitocin comprises the following steps:

1) pretreating ansamitocin fermentation liquor, and then adding a filter aid for filter pressing to obtain hypha residues;

2) leaching the mycelium residues in the step 1) by using a polar solvent, and filtering to obtain a leaching solution;

3) concentrating to remove the solvent in the leaching liquor in the step 2) to obtain a concentrated solution, and then adding an extractant which is not dissolved in water into the concentrated solution for extraction;

4) concentrating the extracted organic phase, cooling and crystallizing to obtain crude ansamitocin A;

5) crystallizing the crude product A in the step 4) by using a mixed solvent to obtain a crude product B, wherein the mixed solvent is a combination of any one of ethyl acetate and dichloromethane and any one of n-hexane and n-heptane;

6) crystallizing the crude product B by using a polar mixed solvent to obtain a crystal C, and drying to obtain a high-purity ansamitocin finished product, wherein the polar mixed solvent is the combination of any one of methanol, ethanol and acetone and water;

the pretreatment in the step 1) is to adjust the pH of ansamitocin fermentation liquor to 2.0-5.0. Preferably, the pH of the ansamitocin fermentation liquor is adjusted to 3.8-4.2. By adjusting the pH value, the protein and the mycelium in the ansamitocin fermentation liquor can be aggregated into clusters, and the filtration efficiency is improved.

The filter aid in the step 1) is perlite or diatomite.

And (2) correspondingly adding 20-60 g of filter aid into each liter of ansamitocin fermentation liquor in the step 1). Preferably, 30-50 g of filter aid is correspondingly added into each liter of ansamitocin fermentation liquor.

The polar solvent in the step 2) is any one or more of methanol, ethanol, acetone and ethyl acetate, and 2-5L of polar solvent is used for leaching per kilogram of hypha residues. Preferably, 3-4L of polar solvent is used for leaching per kilogram of hypha residues.

The leaching time in the step 2) is 6-15 h. Preferably, the leaching time is 6-8 h.

And 3) in the step 3), the extractant incompatible with water is ethyl acetate, butyl acetate, dichloromethane or toluene, the addition amount of the extractant is 0.5-2 times of the volume of the concentrated solution, and the extraction time is 1-5 h. Preferably, the addition amount of the extracting agent is 1-2 times of the volume of the concentrated solution, and the extraction time is 4-5 hours.

Cooling to 0-30 ℃ in the step 4) for crystallization. Preferably, the temperature is reduced to 20-30 ℃.

The drying in step 6) is drying under reduced pressure.

The temperature of the reduced pressure drying is 30-60 ℃, and the time is 8-16 h.

In the step 5), the volume ratio of any one of ethyl acetate and dichloromethane to any one of n-hexane and n-heptane is 1: 0.5 to 5.

And 6), in the polar mixed solvent, the volume ratio of methanol, ethanol or acetone to water is 1: 0.1 to 5.

The concentration in step 3) and step 4) is reduced pressure concentration. The temperature of the reduced pressure concentration is 45-60 ℃.

The concentration of ansamitocins in the system before crystallization after concentration in the step 4) is 10-30 g/L. Preferably 20 to 30 g/L.

Compared with the prior art, the preparation method of high-purity ansamitocin has the main advantages that the strong polar impurities and the non-chromatographic impurities influencing the content in the crude product A after the extraction and the first crystallization treatment by the extracting agent are almost completely removed, and the product content is greatly improved; then removing most pigments through second crystallization and adjusting the component proportion of P-2, P-4 and the like through third polar mixed solvent crystallization, wherein the single-component HPLC purity of the ansamitocin P-3 reaches 92.8 percent, and the total purity of the ansamitocin multi-component HPLC reaches 99.6 percent. In addition, the method does not use a silica gel purification process, does not consume a large amount of solvent and generate solid wastes such as waste silica gel and the like, is environment-friendly and saves a large amount of cost.

The preparation method of high-purity ansamitocin not only improves the product quality, but also greatly reduces the requirements of process operation and equipment, reduces the production cost, is environment-friendly and is suitable for industrial production.

Drawings

FIG. 1 is an HPLC chromatogram of the leach liquor of example 1;

FIG. 2 is an HPLC chromatogram of crude ansamitocin A of example 1;

FIG. 3 is an HPLC chromatogram of white crystals of ansamitocin of example 1.

Detailed Description

The invention will be further illustrated with reference to the following specific examples. It is to be understood that these examples are for illustrative purposes only and are not meant to be any limitation on the present invention.

Example 1

The preparation method of high-purity ansamitocin comprises the following steps:

1) putting the ansamitocin fermentation liquor into a tank for 4000L, wherein the fermentation unit is 624ug/ml (the ansamitocin content is 2496g), adjusting the pH of the ansamitocin fermentation liquor to 4.05 by adopting hydrochloric acid, then adding 120kg of perlite, and performing plate-and-frame filter pressing to obtain 739kg of hypha residues;

2) leaching the mycelium residues in the step 1) with 3200L of ethanol, stirring for 8h, and performing filter pressing to obtain 3500L of leaching solution, wherein the concentration of ansamitocin is 606ug/ml (the mass of the ansamitocin is 2121g), performing HPLC analysis on the leaching solution, and the HPLC map is shown in Table 1, wherein the specific data is shown in Table 1, and the purity of ansamitocin P-3 is 74.8% in the process shown in Table 1;

3) concentrating the leaching solution in the step 2) at 50 ℃ until no solvent flows out, adding about 1600L of residual butyl acetate, stirring and extracting at room temperature for 2h, and separating liquid to obtain 900L of extracted organic phase, wherein the concentration of ansamitocin is 2056ug/ml (the mass of ansamitocin is 1850 g);

4) concentrating the extracted organic phase in the step 3), cooling to 20 ℃ and crystallizing to obtain crude ansamitocin A3110 g, wherein the content of a target ansamitocin P-3 in the crude ansamitocin A is 56% (1741 g); performing HPLC analysis on ansamitocin, wherein the HPLC map is shown in table 2, the specific data are shown in table 2, the data in table 2 can obtain the purity of ansamitocin P-3 of 84.6%;

5) adding the crude product A into a mixed solvent of 35L ethyl acetate and n-hexane (the volume ratio of ethyl acetate to n-hexane is 1: 1) crystallization was carried out to obtain crude product B2295 g, in which the ansamitocin P-3 content in the crude product was 63% (1446 g);

6) to the crude product B was added 40L of an aqueous acetone solution (volume ratio of acetone to water 1: 1.67) to obtain the wet crystal C1354g with ansamitocin P-3 content of 58% (785 g);

7) and (3) drying the hygromycin C at 45 ℃ under reduced pressure for 12 hours to obtain 959g of high-purity ansamitocin white crystals, and performing HPLC analysis on the high-purity ansamitocin white crystals, wherein an HPLC map is shown in a figure 3, data of the HPLC map is shown in a table 3, the purity of ansamitocin P-3 is 92.8%, the total purity of ansamitocin multicomponent is 99.6%, the content of P-3 single component is 92.0% (882g), and the total yield is 35.3%.

The HPLC detection method for ansamitocins in this example is as follows:

reversed phase HPLC analysis using C18(4um, 4.6 x 250mm) column at 40 ℃; gradient eluting with acetonitrile (B) -water (A) system at flow rate of 1.0ml/min, detecting under UV at 234nm, and running for 50 min; the sample to be tested is dissolved into the concentration of about 500ug/ml by using acetonitrile, and the sample injection amount is 20 ul.

The gradient elution is specifically as follows: 0min, 55% of phase A and 45% of phase B; 20min, 55% of phase A and 45% of phase B; 35min, 20% of phase A and 80% of phase B; 40min, 20% of phase A and 80% of phase B; 41min, 55% phase A, 45% phase B; 50min, 55% phase A, 45% phase B.

TABLE 1 HPLC data for ansamitocin leach liquors

TABLE 2 HPLC data for crude ansamitocin A

TABLE 3 HPLC data for high purity ansamitocin white crystals

Peak number	Name of Compound	Retention time (min)	Area (mAU s)	Area%
					1		5.946	4.30497	0.0322
2		9.141	4.47450	0.0335
					3	AP-2	13.573	715.95087	5.3581
4		14.582	12.70156	0.0951
					5	AP-3	18.101	1.23964e4	92.7739
6	AP-3'	19.237	100.97854	0.7557
					7		25.329	33.90541	0.2537
8	AP-4	26.318	93.22408	0.6977
					Total of			1.33619e4	100.0000

Example 2

The preparation method of high-purity ansamitocin comprises the following steps:

1) putting ansamitocin fermentation liquor into a tank of 4300L, wherein the fermentation unit is 574ug/ml (the ansamitocin content is 2648g), adjusting the pH of the ansamitocin fermentation liquor to 4.02 by using hydrochloric acid, adding 120kg of perlite, and performing plate-frame filter pressing to obtain 726kg of mycelium residues;

2) leaching the hypha residues in the step 1) with 2600L ethanol, stirring for 6h, and performing filter pressing to obtain 3700L of leaching solution with the concentration of the ansamitocin of 594ug/ml (the mass of the ansamitocin is 2198 g);

3) concentrating the leaching liquor in the step 2) at 50 ℃ until no solvent flows out, adding 1500L of toluene, stirring and extracting at room temperature for 4h, and separating liquid to obtain 1060L of extracted organic phase, wherein the concentration of ansamitocin is 1692ug/ml (the mass of ansamitocin is 1793 g);

4) concentrating the extracted organic phase in the step 3), cooling to 30 ℃ and crystallizing to obtain crude product A3464 g and ansamitocin P-3 content 49% (1697 g);

5) the crude product a was added to 35L of an ethyl acetate-n-heptane mixed solvent (volume ratio of ethyl acetate to n-heptane 1: 2.5) crystallization to obtain crude product B2710 g and ansamitocin P-3 content of 60% (1626 g);

6) to the crude product B was added 26L of an aqueous acetone solution (volume ratio of acetone to water 1: 1) crystallizing to obtain wet crystal C1461g with ansamitocin P-3 content of 77% (1125 g);

7) and drying the hygromycin C at 45 ℃ under reduced pressure for 12 hours to obtain 1198g of high-purity ansamitocin white crystals, 92.7% of ansamitocin P-3, 99.5% of total purity of ansamitocin multicomponent, 93.6% of content of P-3 single component (1121g), and 42.3% of total yield.

Example 3

The preparation method of high-purity ansamitocin comprises the following steps:

1) putting the ansamitocin fermentation liquor into a tank for 3800L, wherein the fermentation unit is 585ug/ml (the ansamitocin content is 2223g), adjusting the pH of the ansamitocin fermentation liquor to 3.8 by adopting hydrochloric acid, then adding 120kg of perlite, and performing plate-and-frame filter pressing to obtain 698kg of mycelium residues;

2) leaching the mycelium residues in the step 1) with 3800L ethanol, stirring for 8h, and press-filtering to obtain 3800L leachate with an ansamitocin concentration of 495ug/ml (the mass of the ansamitocin is 1881 g);

3) concentrating the leaching liquor in the step 2) at 50 ℃ until no solvent flows out, adding 1200L of toluene, stirring and extracting at room temperature for 1h, and separating liquid to obtain 1300L of an extracted organic phase, wherein the concentration of ansamitocin is 1432ug/ml (the mass of ansamitocin is 1862 g);

4) concentrating the extracted organic phase in the step 3), cooling to 10 ℃ and crystallizing to obtain crude product A2760 g and ansamitocin P-3 content 59% (1628 g);

5) adding the crude product A into a mixed solvent of 35L ethyl acetate and n-hexane (the volume ratio of ethyl acetate to n-hexane is 1: 0.75) to obtain 2469g of crude product B with 61% of ansamitocin P-3 (1506 g);

6) to the crude product B was added 30L of an aqueous acetone solution (volume ratio of acetone to water 1: 2) crystallization was carried out to obtain hygrometric C1391g, 71% (988g) of ansamitocin P-3;

7) the hygromycin C was dried at 45 ℃ under reduced pressure for 10 hours to obtain 959g of high-purity ansamitocin white crystals, 94.0% of ansamitocin P-3, 99.4% of total purity of ansamitocin multicomponent, 94.2% of P-3 monocomponent content (903g), and 40.6% of total yield.

In other embodiments of the invention, the filter aid perlite in step 1) may be replaced with diatomaceous earth.

In other embodiments of the present invention, the mycelium residue may be leached in step 2) with methanol, acetone or ethyl acetate.

In other embodiments of the present invention, the extractant in step 3) may also be ethyl acetate or dichloromethane.

In other embodiments of the present invention, the mixed solvent in step 5) may also be dichloromethane-n-hexane or dichloromethane-n-heptane.

In other embodiments of the present invention, the polar mixed solvent in step 6) is an aqueous solution of methanol or an aqueous solution of ethanol.

Various other modifications and changes may be made by those skilled in the art based on the above-described technical solutions and concepts, and all such modifications and changes should fall within the scope of the claims of the present invention.