阅读说明：本技术 一种益生菌冻干粉及其制备方法 (Probiotic freeze-dried powder and preparation method thereof ) 是由 信朝阳 于 2021-10-11 设计创作，主要内容包括：本发明属于益生菌制剂技术领域,公开了一种益生菌冻干粉及其制备方法。该益生菌冻干粉富含多种益生元、多种膳食纤维和多种维生素,精选多种菌株,采用多层包埋技术获得的成品。该成品中含有超过18000亿CFU。该益生菌冻干粉具有调节肠胃、补充维生素和多种微量元素、明显增加了吸收率,促进吸收的同时也有助于提高使用者的免疫力。可加速小肠内食物的分解,抑制腐败菌的生存繁殖,改变了蛋白质的代谢途径,消除氨气、硫化氢等挥发性有机气体的释放,同时,口感好,易于肠道吸收的矿物质、维生素、膳食纤维。(The invention belongs to the technical field of probiotic preparations, and discloses probiotic freeze-dried powder and a preparation method thereof. The probiotic freeze-dried powder is rich in various prebiotics, various dietary fibers and various vitamins, and a finished product is obtained by selecting various bacterial strains and adopting a multilayer embedding technology. The finished product contains more than 18000 hundred million CFU. The probiotic freeze-dried powder has the effects of regulating intestines and stomach, supplementing vitamins and various trace elements, obviously increasing the absorption rate, promoting the absorption and simultaneously being beneficial to improving the immunity of a user. Can accelerate the decomposition of food in the small intestine, inhibit the survival and propagation of putrefying bacteria, change the metabolic pathway of protein, eliminate the release of volatile organic gases such as ammonia gas, hydrogen sulfide and the like, and simultaneously has good taste and is easy to absorb mineral substances, vitamins and dietary fibers by the intestinal tract.)

1. The probiotic freeze-dried powder is characterized by being mainly prepared from the following raw materials in parts by mass:

30-220 parts of probiotics, 20-150 parts of dietary supplements, 20-150 parts of vitamins, 15-110 parts of trace elements, 70-250 parts of milk powder, 20-60 parts of oligolactose and 20-60 parts of pectin.

2. The probiotic freeze-dried powder according to claim 1, which is prepared from the following raw materials in parts by mass:

80-150 parts of probiotic agent, 60-100 parts of dietary supplement, 60-100 parts of vitamin, 40-75 parts of trace element, 100-180 parts of milk powder, 25-45 parts of oligolactose and 25-45 parts of pectin.

3. The probiotic freeze-dried powder according to claim 2, which is prepared from the following raw materials in parts by mass:

100 parts of probiotics, 80 parts of dietary supplements, 80 parts of vitamins, 50 parts of trace elements, 120 parts of milk powder, 30 parts of oligolactose and 30 parts of pectin.

4. The probiotic freeze-dried powder according to any one of claims 1 to 3, wherein the probiotic comprises Lactobacillus plantarum, Bifidobacterium and Pediococcus acidilactici;

the lactobacillus plantarum comprises one or more of lactobacillus plantarum Lp90, lactobacillus plantarum N13, lactobacillus plantarum CN2018, lactobacillus plantarum CW006, lactobacillus reuteri LR08, lactobacillus rhamnosus LRa05, lactobacillus casei LC89, lactobacillus reuteri DSM17938, lactobacillus rhamnosus LGG, lactobacillus fermentum CECT5716, and lactobacillus acidophilus LA 85;

the Bifidobacterium comprises one or more of Bifidobacterium longum BL21, Bifidobacterium animalis Bb-12, Bifidobacterium lactis BLA80, Bifidobacterium adolescentis BAC30, Bifidobacterium breve M-16V, Bifidobacterium bifidum BBi32, Bifidobacterium lactis HN019 and Bifidobacterium infantis BI 45;

the Pediococcus acidilactici is Pediococcus acidilactici CCFM 7902.

5. A probiotic freeze-dried powder according to any of claims 1 to 3, wherein the dietary supplement comprises one or more of stachyose, polydextrose, soy oligosaccharides, isomaltooligosaccharides, crystalline fructose, resistant dextrin, erythritol, lactulose, chitooligosaccharides, polyfructose, fructooligosaccharides and galactooligosaccharides.

6. A probiotic freeze-dried powder according to any one of claims 1 to 3, wherein the vitamins include one or more of vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, niacin, pantothenic acid, folic acid, beta-carotene, vitamin a, vitamin D and vitamin E.

7. The probiotic freeze-dried powder according to any one of claims 1 to 3, wherein the trace elements include one or more of calcium carbonate, zinc gluconate, ferrous gluconate and selenium-enriched yeast.

8. A probiotic freeze-dried powder according to any of claims 1 to 3, characterized in that the milk powder comprises a mixture of bovine colostrum powder and milk powder;

the ratio of the bovine colostrum powder to the milk powder is 0.1-0.3 wt%: 10-40 wt%.

9. A method for preparing a probiotic freeze-dried powder according to any one of claims 1 to 8, characterized in that the method comprises the following steps: selecting probiotic agent according to corresponding proportion, adding solvent, heating, adding dietary supplement, vitamins, microelements, milk powder, oligolactose and pectin, mixing well, drying, sterilizing to obtain the final product.

10. The method for preparing probiotic freeze-dried powder according to claim 9, wherein the solvent is distilled water; the ratio of the probiotic to the solvent is 1: 0.5-0.8 wt%;

heating to 25-28 ℃;

the drying temperature is 40-45 ℃;

the finished product is solid powder.

Technical Field

The invention belongs to the technical field of probiotic preparations, and particularly relates to probiotic freeze-dried powder and a preparation method thereof.

Background

Probiotics are active microorganisms beneficial to a host, can improve the micro-ecological balance of the host and play a beneficial role by being planted in the intestinal tract and the reproductive system of a human body, and the health care effect of the probiotics is generally accepted and widely applied along with the improvement of the living standard of people. Research and product development of probiotics becomes a trend, and research and development in the field are carried out. The production is particularly prominent in the research and application aspects of lactobacillus and bifidobacterium at present, and products in the aspects comprise traditional yoghourt, various newly developed yoghourt, lactic acid beverages, various single compound bacteria health-care products and foods, and probiotic preparations for treating intestinal diseases, because the storage conditions of the probiotics are harsh.

The digestion and absorption of food are important components of human health, and compared with the pore structure of human small intestine villi, various foods after chewing are also macromolecules, and probiotics are needed to help decompose into small molecules to be digested and absorbed. If these nutrients are decomposed by harmful bacteria, an offensive odor and toxins are generated. The odor and peculiar smell generated by decomposition of harmful bacteria mainly comprise volatile gases such as ammonia gas, methane, hydrogen sulfide and the like.

The freeze-dried powder is prepared by freezing the water in the liquid medicine in advance by adopting a vacuum freeze-drying method of a freeze dryer, and then sublimating the frozen water in the liquid medicine in a vacuum sterile environment, thereby obtaining freeze drying. In short, the water in the liquid medicine is pumped out in a low-temperature environment, and the original medicinal effect is kept.

However, the existing products generally have the defects that live bacteria are easy to die and the number of the bacteria is unstable, so that the shelf life of the probiotic product is short.

Disclosure of Invention

In order to solve the problems in the prior art, the invention aims to provide probiotic freeze-dried powder and a preparation method thereof.

The technical scheme adopted by the invention is as follows: the probiotic freeze-dried powder is mainly prepared from the following raw materials in parts by mass:

30-220 parts of probiotics, 20-150 parts of dietary supplements, 20-150 parts of vitamins, 15-110 parts of trace elements, 70-250 parts of milk powder, 20-60 parts of oligolactose and 20-60 parts of pectin.

Preferably, the probiotic freeze-dried powder is mainly prepared from the following raw materials in parts by mass:

80-150 parts of probiotic agent, 60-100 parts of dietary supplement, 60-100 parts of vitamin, 40-75 parts of trace element, 100-180 parts of milk powder, 25-45 parts of oligolactose and 25-45 parts of pectin.

Preferably, the probiotic freeze-dried powder is mainly prepared from the following raw materials in parts by mass:

100 parts of probiotics, 80 parts of dietary supplements, 80 parts of vitamins, 50 parts of trace elements, 120 parts of milk powder, 30 parts of oligolactose and 30 parts of pectin.

Preferably, the probiotic comprises lactobacillus plantarum, bifidobacterium and pediococcus acidilactici;

the lactobacillus plantarum comprises one or more of lactobacillus plantarum Lp90, lactobacillus plantarum N13, lactobacillus plantarum CN2018, lactobacillus plantarum CW006, lactobacillus reuteri LR08, lactobacillus rhamnosus LRa05, lactobacillus casei LC89, lactobacillus reuteri DSM17938, lactobacillus rhamnosus LGG, lactobacillus fermentum CECT5716, and lactobacillus acidophilus LA 85;

the Bifidobacterium comprises one or more of Bifidobacterium longum BL21, Bifidobacterium animalis Bb-12, Bifidobacterium lactis BLA80, Bifidobacterium adolescentis BAC30, Bifidobacterium breve M-16V, Bifidobacterium bifidum BBi32, Bifidobacterium lactis HN019 and Bifidobacterium infantis BI 45;

the Pediococcus acidilactici is Pediococcus acidilactici CCFM 7902.

Preferably, the dietary supplement comprises one or more of stachyose, polydextrose, soy oligosaccharides, isomaltooligosaccharides, crystalline fructose, resistant dextrins, erythritol, lactulose, chitooligosaccharides, polyfructose, fructooligosaccharides and galactooligosaccharides.

Stachyose is a naturally occurring tetrasaccharide, and is a functional oligosaccharide which can obviously promote the proliferation of beneficial bacteria such as bifidobacterium and the like. The pure product is white powder, has slightly sweet taste, sweetness of 22 percent of that of the cane sugar, pure taste and no bad taste or peculiar smell. The molecular structure is as follows: "galactose-glucose-fructose". Stachyose has obvious proliferation effect on beneficial bacteria such as bifidobacterium, lactobacillus and the like in gastrointestinal tract of human body, and can quickly improve the environment in the digestive tract of human body and regulate the balance of microecological bacteria. It can promote the formation of dominant bacteria status of beneficial bacteria in digestive tract, inhibit the production of putrefying bacteria such as Clostridium acidogenum and the like, produce a large amount of physiological active substances, regulate the pH value of intestinal tract, kill pathogenic bacteria, inhibit the generation of putrefying products, inhibit the generation and absorption of endogenous carcinogens, and decompose and derive multiple immune function factors. Commonly known as: food containing beneficial bacteria. Stachyose is a functional oligosaccharide, and does not have any dependence. As the beneficial bacteria in the intestinal tract are also like the beneficial bacteria, the beneficial bacteria are eaten every day, and if the beneficial bacteria are not eaten in one day, the beneficial bacteria are possibly also in malnutrition, and if the beneficial bacteria are not eaten for a long time, the beneficial bacteria are starved to death. Targeting colonization has obvious effect. Compared with probiotic products such as yoghourt and the like: most of the bacteria of the bifidobacteria are inactivated when the bifidobacteria pass through gastric acid, and the bacteria are very sensitive to oxygen and rarely reach the intestinal tract really; stachyose has good stability, and can directly colonize each part of intestinal tract. Has quick action, safety and reliability. In contrast to normal prebiotics: can proliferate beneficial bacteria such as Bifidobacterium at 40 times increasing speed, with quick effect and without abdominal distention and discomfort. Is extracted from natural plants, is safe and has no side effect. The function is as follows: 1. stachyose can effectively prevent and relieve constipation and diarrhea of infants, and inhibit intestinal diseases. 2. Stachyose can enhance infantile immunity and improve infantile disease resistance. 3. Stachyose has effects of clearing intestinal tract, removing toxic substance and removing lead. 4. Stachyose can replace metal ions, thereby promoting the absorption of minerals such as calcium.

The soybean oligosaccharide, oligosaccharide (or Oligosaccharides) refers to a general term for saccharides having a molecular structure formed by connecting 2 to 10 monosaccharide molecules by glycosidic bonds. The molecular weight is 300-2000, which is between monosaccharide (glucose, fructose, galactose) and polysaccharide (fiber, starch), and the molecular weight is composed of disaccharide, trisaccharide and tetrasaccharide. Oligosaccharides as "specific health foods" are oligosaccharides having a special biological function and particularly beneficial to gastrointestinal health, and are also called "functional oligosaccharides". The soybean oligosaccharide is a general term for soluble sugar in soybeans. The main components are sucrose (disaccharide) with monosaccharide number of 3-4, raffinose (trisaccharide), stachyose (tetrasaccharide) and the like. 1. Defecation and bowel cleaning: constipation is mostly caused by the lack of bifidobacteria in the intestine. Especially for the elderly. With age. The intestinal bifidobacteria are gradually reduced, and constipation is easy to occur. Tests prove that healthy people can promote the growth of bifidobacterium and have the function of relaxing the bowels by taking 3 g of soybean oligosaccharide every day. The soybean oligosaccharide can also promote enterokinesia and accelerate excretion. 2. Promoting the proliferation of bifidobacteria in intestinal tract: experimental studies prove that after 10-15 g of soybean oligosaccharide is taken every day, the content of bifidobacterium can be increased from 0.99% to 45% after 17 days. The bifidobacteria in the intestine are particularly susceptible to the use of soy oligosaccharides, producing acetic and lactic acids and some antibiotic substances, thus inhibiting the proliferation of exogenous pathogenic bacteria and intestinal putrefying bacteria: the bifidobacteria also form a protective biological and membrane barrier with the intestinal mucosa surface through phosphatidic acid, thereby preventing invasion and colonization of harmful microorganisms. 3. Reduction of serum cholesterol: the bifidobacterium directly influences and interferes with the activity of p-glycosyl-p-methylglutaryl enzyme A reductase, inhibits the synthesis of cholesterol and reduces serum cholesterol. 4. And protection of the liver: the long-term intake of soybean oligosaccharide can reduce the generation of toxic metabolites in vivo and relieve the detoxifying burden of liver, so the soybean oligosaccharide has certain effects on preventing and treating hepatitis and preventing liver cirrhosis. 5. Inhibition of the production of intestinal putrefactive substances: preventing and treating constipation and abdominal diseases. The function and the effect are as follows: improving the ecological environment of intestinal tract and proliferating bifidobacteria. Preventing constipation, preventing intestinal melanism and enteroparalysis caused by intestinal lavage, inhibiting harmful bacteria, improving disease resistance, reducing blood lipid, regulating blood pressure, and correcting monophagia and anorexia of children. Promoting calcium and zinc absorption, preventing osteoporosis, promoting vitamin synthesis, and resisting aging.

The isomaltose hypgather and isomaltose hypgather pulp (powder) have good taste and quality, can be used for replacing part of cane sugar, improve the taste of food and reduce the sweetness. The viscosity of the isomaltose hypgather pulp is very close to that of the sucrose solution with the same concentration, the handling is easier than that of maltose when food is processed, and the structure and the characteristics of the food such as candy, cake and the like are not affected. The isomaltooligosaccharide has excellent heat resistance and acid resistance. The syrup with concentration of 50% is not decomposed by heating at pH3 and 120 deg.C for a long time. Can be used in beverage, canned food, high-temperature-treated food or low-pH food to maintain original properties and functions. Isomaltose hypgather has moisture retention property, makes water not easy to evaporate, has good effect on moisture retention and quality maintenance of various foods, and can inhibit formation of crystals of sucrose and glucose. Starch-based foods such as breads and desserts are often hardened upon storage, and the addition of isomaltooligosaccharides prevents starch retrogradation and prolongs the shelf life of the food. The isomaltooligosaccharide has a reducing group at the end of the sugar molecule, and undergoes Maillard reaction when being heated together with protein or amino acid to produce unbutton or divest one's upper garment coloration, wherein the degree of coloration depends on the sugar concentration, and on the type, pH, heating temperature and time of the heated protein or amino acid. Therefore, the above factors should be considered in processing various foods using isomaltooligosaccharides. Isomaltooligosaccharide water activity: at a concentration of 75% and 25 ℃ 0.75. Close to sucrose, it is convenient to use a conversion in the food formulation that replaces part of the sucrose.

The crystalline fructose is obtained by crystallizing a mixture of fructose,

preferably, the vitamins include one or more of vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, niacin, pantothenic acid, folic acid, beta-carotene, vitamin a, vitamin D, and vitamin E.

Preferably, the trace elements include one or more of calcium carbonate, zinc gluconate, ferrous gluconate, and selenium-enriched yeast.

Preferably, the milk powder comprises a mixture of bovine colostrum powder and milk powder;

the ratio of the bovine colostrum powder to the milk powder is 0.1-0.3 wt%: 10-40 wt%.

The bovine colostrum powder contains immunoglobulin IgG, and the addition amount is 1%.

A preparation method of probiotic freeze-dried powder comprises the following steps: selecting probiotic agent according to corresponding proportion, adding solvent, heating, adding dietary supplement, vitamins, microelements, milk powder, oligolactose and pectin, mixing well, drying, sterilizing to obtain the final product.

Preferably, the solvent is distilled water; the ratio of the probiotic to the solvent is 1: 0.5-0.8 wt%;

heating to 25-28 ℃;

the drying temperature is 40-45 ℃;

the finished product is solid powder.

The invention has the beneficial effects that:

the invention provides a probiotic freeze-dried powder which is a finished product which is rich in various prebiotics, various dietary fibers and various vitamins, is prepared by selecting various bacterial strains and adopting a multilayer embedding technology. The finished product contains more than 18000 hundred million CFU.

The probiotic freeze-dried powder has the effects of regulating intestines and stomach, supplementing vitamins and various trace elements, obviously increasing the absorption rate, promoting the absorption and simultaneously being beneficial to improving the immunity of a user. Can accelerate the decomposition of food in the small intestine, inhibit the survival and propagation of putrefying bacteria, change the metabolic pathway of protein, eliminate the release of volatile organic gases such as ammonia gas, hydrogen sulfide and the like, and simultaneously has good taste and is easy to absorb mineral substances, vitamins and dietary fibers by the intestinal tract.

Detailed Description

The present invention is further illustrated below with reference to specific examples. It will be appreciated by those skilled in the art that the following examples, which are set forth to illustrate the present invention, are intended to be part of the present invention, but not to be construed as limiting the scope of the present invention. The reagents used are all conventional products which are commercially available.

Example 1:

a preparation method of probiotic freeze-dried powder comprises the following steps: selecting 30 g of probiotics according to a corresponding proportion, adding 6 g of distilled water, heating to 25 ℃, adding 20 g of dietary supplement, 20 g of vitamin, 15 g of trace element, 70 g of milk powder, 20 g of oligolactose and 20 g of pectin, uniformly mixing, drying at 40 ℃, and sterilizing to obtain a solid powdery finished product.

Example 2:

a preparation method of probiotic freeze-dried powder comprises the following steps: selecting 220 g of probiotics according to a corresponding proportion, adding 20 g of distilled water, heating to 28 ℃, adding 150 g of dietary supplement, 150 g of vitamin, 110 g of trace element, 250 g of milk powder, 60 g of oligolactose and 60 g of pectin, uniformly mixing, drying at 45 ℃, and sterilizing to obtain a solid powdery finished product.

Example 3:

a preparation method of probiotic freeze-dried powder comprises the following steps: selecting 80 g of probiotics according to a corresponding proportion, adding 40 g of distilled water, heating to 25 ℃, adding 60 g of dietary supplement, 60 g of vitamin, 40 g of trace element, 100 g of milk powder, 25 g of oligolactose and 25 g of pectin, uniformly mixing, drying at 40 ℃, and sterilizing to obtain a solid powdery finished product.

Example 4:

a preparation method of probiotic freeze-dried powder comprises the following steps: selecting 150 g of probiotics according to a corresponding proportion, adding 75 g of distilled water, heating to 28 ℃, adding 100 g of dietary supplement, 100 g of vitamin, 75 g of trace element, 180 g of milk powder, 45 g of oligolactose and 45 g of pectin, uniformly mixing, drying at 45 ℃, and sterilizing to obtain a solid powdery finished product.

Example 5:

a preparation method of probiotic freeze-dried powder comprises the following steps: selecting 100 g of probiotics according to a corresponding proportion, adding 50 g of distilled water, heating to 25 ℃, adding 80 g of dietary supplement, 80 g of vitamin, 50 g of trace elements, 120 g of milk powder, 30 g of oligolactose and 30 g of pectin, uniformly mixing, drying at 40 ℃, and sterilizing to obtain a solid powdery finished product.

In the above embodiment, the selected probiotic includes lactobacillus plantarum, bifidobacterium and pediococcus acidilactici;

the Lactobacillus plantarum comprises Lactobacillus plantarum Lp90, Lactobacillus plantarum N13, Lactobacillus plantarum CN2018, Lactobacillus plantarum CW006, Lactobacillus reuteri LR08, Lactobacillus rhamnosus LRa05, Lactobacillus casei LC89, Lactobacillus reuteri DSM17938, Lactobacillus rhamnosus LGG, Lactobacillus fermentum CECT5716 and Lactobacillus acidophilus LA 85;

the Bifidobacterium includes Bifidobacterium longum BL21, Bifidobacterium animalis Bb-12, Bifidobacterium lactis BLA80, Bifidobacterium adolescentis BAC30, Bifidobacterium breve M-16V, Bifidobacterium bifidum BBi32, Bifidobacterium lactis HN019 and Bifidobacterium infantis BI 45; the Pediococcus acidilactici is Pediococcus acidilactici CCFM 7902.

The vitamins include one or more of vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, nicotinic acid, pantothenic acid, folic acid, beta-carotene, vitamin A, vitamin D, and vitamin E.

The microelements comprise one or more of calcium carbonate, zinc gluconate, ferrous gluconate and selenium-rich yeast.

The milk powder comprises mixture of bovine colostrum powder and milk powder.

Applicable objects are as follows: not applicable to infants.

The product specification is as follows: 5g per bag.

Brewing method and edible amount: children under 6 years old are recommended to take 1 time per day, 1 bag each time; the children and adults over 6 years old are 1-2 times daily, and 1 bag each time.

It can be taken after concocting with warm water or milk below 37 deg.C, or concocting with complementary food.

The table of the nutrient components contained in the product is as follows:

the present invention is not limited to the above alternative embodiments, and any other products in various forms can be obtained by the present invention, and the present invention is within the protection scope of the present invention. The above embodiments should not be construed as limiting the scope of the present invention, and it will be understood by those skilled in the art that modifications may be made to the technical solutions described in the above embodiments, or equivalent substitutions may be made to some or all of the technical features thereof, without departing from the scope of the present invention, and at the same time, such modifications or substitutions may not make the essence of the corresponding technical solutions depart from the scope of the embodiments of the present invention.