阅读说明：本技术 表达载体元件组合、新的生产用细胞产生方法及其在重组产生多肽中的用途 (Expression vector element combinations, novel production cell production methods and their use in the recombinant production of polypeptides ) 是由 P·M·许尔斯曼 H·克内根 于 2012-12-19 设计创作，主要内容包括：本文报道,对于瞬时转染,人延伸因子1α启动子(含有内含子A)的使用提供增强的生产力(在LC-HC-SM组织中),与使用SV40polyA信号序列相比,牛生长激素polyA信号序列的使用提供增强的生产力,在包含hCMV启动子的载体中,向bGH PolyA信号序列加入HGT产生提高的生产力,载体组织LC(3`-5′)-HC-SM产生改善的表达。对于稳定库,据报道,用包含hEF1α启动子的载体产生的库在分批分析中显示增强的生产力,用包含hEF1α启动子的载体产生的克隆显示减少的低产克隆数,且用包含hEF1α启动子的载体产生的克隆显示更高的IgG表达稳定性。对于单克隆,据报道,选择标记放置在下游的载体组织(LC-HC-SM)对单克隆的生产力具有积极作用,且用包含bGH polyA信号序列和hGT的载体产生的克隆具有更高的生产力。(Herein is reported that for transient transfection, the use of the human elongation factor 1 alpha promoter (containing intron a) provides enhanced productivity (in LC-HC-SM organization), the use of the bovine growth hormone polyA signal sequence provides enhanced productivity compared to the use of the SV40polyA signal sequence, the addition of HGT to the bGH polyA signal sequence in a vector comprising the hCMV promoter results in enhanced productivity, and the vector organization LC (3 '-5') -HC-SM results in improved expression. For stable pools, it was reported that pools produced with vectors containing the hEF1 α promoter showed enhanced productivity in batch analysis, clones produced with vectors containing the hEF1 α promoter showed reduced numbers of low-producing clones, and clones produced with vectors containing the hEF1 α promoter showed higher stability of IgG expression. For the monoclonal, it was reported that the vector organization (LC-HC-SM) with the selectable marker placed downstream had a positive effect on the productivity of the monoclonal, and that clones generated with the vector containing the bGH polyA signal sequence and hGT had higher productivity.)