阅读说明：本技术 一种大蒜蛋白酶解物及其制备方法 (Garlic protein zymolyte and preparation method thereof ) 是由 李成秀 张宗方 于 2021-02-06 设计创作，主要内容包括：本发明属于蛋白酶制备技术领域,公开了一种大蒜蛋白酶解物,为一种含有3～9肽的活性多肽类化合物和多糖类化合物的混合物,其干物质的组分及重量百分比为多肽类化合物含量为71～78％,多糖类化合物含量为7～14％,余量为氨基酸,还公开了一种大蒜蛋白酶解物制备方法,包括以下步骤：将洁净的大蒜粉碎,获得大蒜粉末,将所述大蒜粉末与水体混合形成大蒜混合溶液,将大蒜混合溶液放置于除味装置内进行除味,本发明通过除味装置进行除味,从而去除大蒜混合溶液中的臭味,避免大蒜混合溶液产生异味,从而不会影响大蒜蛋白酶解物的制备,降低了蛋白酶解物的制备难度,从而提高了蛋白酶解物的制备效率。(The invention belongs to the technical field of protease preparation, and discloses a garlic protein zymolyte which is a mixture of an active polypeptide compound containing 3-9 peptides and a polysaccharide compound, wherein the dry matter comprises 71-78 wt% of the polypeptide compound, 7-14 wt% of the polysaccharide compound and the balance of amino acid, and a preparation method of the garlic protein zymolyte comprises the following steps: clean garlic is crushed to obtain garlic powder, the garlic powder is mixed with water to form garlic mixed solution, and the garlic mixed solution is placed in a smell removal device for smell removal.)

1. The garlic protein zymolyte is characterized by being a mixture of an active polypeptide compound containing 3-9 peptides and a polysaccharide compound, wherein the dry matter comprises 71-78 wt% of the polypeptide compound, 7-14 wt% of the polysaccharide compound and the balance of amino acid.

2. A preparation method of garlic protein zymolyte is characterized by comprising the following steps:

s1, crushing clean garlic to obtain garlic powder, mixing the garlic powder with a water body to form a garlic mixed solution, and placing the garlic mixed solution in a smell removal device for removing smell;

s2, adding an enzymolysis solution and alkali liquor into the deodorized garlic mixed solution, adjusting the pH value to 7.6-8.4, and carrying out enzymolysis on the deodorized garlic mixed solution to form a garlic enzymolysis solution;

and S3, separating and filtering the garlic enzymolysis solution to form a liquid-phase enzymolysis substrate and a solid-phase enzymolysis substrate.

3. The method for preparing garlic protein hydrolysate according to claim 2, wherein the method comprises the following steps: the method comprises the following steps of (1) crushing clean garlic to obtain garlic powder, mixing the garlic powder with a water body to form a garlic mixed solution, and placing the garlic mixed solution in a smell removal device for smell removal, wherein the method comprises the following steps:

s11, cleaning mud on the surface of the garlic, peeling the garlic and then cleaning the garlic so as to clean the garlic and obtain clean garlic;

s12, drying the clean garlic to remove water on the surface of the garlic and dry the garlic;

s13, carrying out heat treatment on the garlic for a period of time under a certain temperature condition;

s14, after the garlic is subjected to heat treatment, crushing the garlic through crushing equipment to obtain garlic powder;

s15, placing the garlic powder into a container, adding water into the container according to a certain proportion, and mixing the garlic powder and the water to form a garlic mixed solution.

4. The method for preparing garlic protein hydrolysate according to claim 2, wherein the method comprises the following steps: adding the enzymolysis solution and alkali liquor into the deodorized garlic mixed solution, and carrying out enzymolysis on the deodorized garlic mixed solution to form a garlic enzymolysis solution, wherein the method comprises the following steps:

s21, placing the garlic enzymolysis solution in a shaking device for shaking;

s22, separating the vibrated garlic enzymolysis solution by using separation equipment to obtain a primary liquid-phase enzymolysis substrate and a primary solid-phase enzymolysis substrate;

s23, filtering the primary liquid-phase enzymolysis substrate and the primary solid-phase enzymolysis substrate, so that the primary liquid-phase enzymolysis substrate and the primary solid-phase enzymolysis substrate are separated to obtain a liquid-phase enzymolysis substrate and a solid-phase enzymolysis substrate.

5. The method for preparing garlic protein hydrolysate according to claim 2, wherein the method comprises the following steps: the odor removal device comprises an air extractor or activated carbon.

6. The method for preparing garlic protein zymolyte according to claim 3, which is characterized in that: in the step of carrying out heat treatment on the garlic for a period of time under a certain temperature condition, the certain temperature condition is 58-62 ℃, and the period of time is 18-19 min.

7. The method for preparing garlic protein zymolyte according to claim 4, which is characterized in that: the separation device comprises a centrifuge.

8. The method for preparing garlic protein zymolyte according to claim 3, which is characterized in that: the ratio of the garlic powder to the water body is 8: 100-14: 100, respectively.

9. The method for preparing garlic protein hydrolysate according to claim 2, wherein the method comprises the following steps: the enzymolysis solution is a mixed solution of protease and water.

10. The method for preparing garlic protein zymolyte according to claim 3, which is characterized in that: the alkali liquor is sodium hydroxide solution.

Technical Field

The invention belongs to the technical field of protease preparation, and particularly relates to a garlic protein zymolyte and a preparation method thereof.

Background

Proteases are a generic term for a class of enzymes that hydrolyze peptide chains of proteins, and are classified into endopeptidases and telopeptidases according to the way in which they degrade polypeptides, the former being capable of cleaving large molecular weight polypeptide chains from the middle to form lower molecular weight prions and peptones; the latter can be further classified into carboxypeptidases and aminopeptidases, which hydrolyze peptide chains one by one from the free carboxyl terminus or the free amino terminus of a polypeptide to form amino acids, and according to the manner in which they hydrolyze polypeptides, they can be classified into endopeptidases, which cleave the interior of a protein molecule to form a smaller molecular weight peptide peptone, and exopeptidases, which hydrolyze peptide bonds one by one from the free amino or carboxyl terminus of a protein molecule to free amino acids, the former being aminopeptidases and the latter being carboxypeptidases.

However, the existing preparation method of the garlic protein zymolyte has low preparation efficiency, needs to wait for a long time, greatly increases the preparation difficulty of the garlic protease and reduces the preparation efficiency of the garlic protease.

Disclosure of Invention

The invention aims to provide a garlic protein zymolyte and a preparation method thereof, and aims to solve the problem of low preparation efficiency of garlic protease in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: a garlic protein zymolyte is a mixture of active polypeptide compounds containing 3-9 peptides and polysaccharide compounds, wherein the components and the weight percentage of dry matter are 71-78% of the content of the polypeptide compounds, 7-14% of the content of the polysaccharide compounds and the balance of amino acids.

A preparation method of garlic protein zymolyte comprises the following steps:

s1, crushing clean garlic to obtain garlic powder, mixing the garlic powder with a water body to form a garlic mixed solution, and placing the garlic mixed solution in a smell removal device for removing smell;

s2, adding an enzymolysis solution and alkali liquor into the deodorized garlic mixed solution, adjusting the pH value to 7.6-8.4, and carrying out enzymolysis on the deodorized garlic mixed solution to form a garlic enzymolysis solution;

and S3, separating and filtering the garlic enzymolysis solution to form a liquid-phase enzymolysis substrate and a solid-phase enzymolysis substrate.

Preferably, the method for deodorizing garlic comprises the following steps of:

s11, cleaning mud on the surface of the garlic, peeling the garlic and then cleaning the garlic so as to clean the garlic and obtain clean garlic;

s12, drying the clean garlic to remove water on the surface of the garlic and dry the garlic;

s13, carrying out heat treatment on the garlic for a period of time under a certain temperature condition;

s14, after the garlic is subjected to heat treatment, crushing the garlic through crushing equipment to obtain garlic powder;

s15, placing the garlic powder into a container, adding water into the container according to a certain proportion, and mixing the garlic powder and the water to form a garlic mixed solution.

Preferably, the step of adding the enzymolysis solution and the alkali liquor into the deodorized garlic mixed solution to perform enzymolysis on the deodorized garlic mixed solution to form a garlic enzymolysis solution comprises the following steps:

s21, placing the garlic enzymolysis solution in a shaking device for shaking;

s22, separating the vibrated garlic enzymolysis solution by using separation equipment to obtain a primary liquid-phase enzymolysis substrate and a primary solid-phase enzymolysis substrate;

s23, filtering the primary liquid-phase enzymolysis substrate and the primary solid-phase enzymolysis substrate, so that the primary liquid-phase enzymolysis substrate and the primary solid-phase enzymolysis substrate are separated to obtain a liquid-phase enzymolysis substrate and a solid-phase enzymolysis substrate.

Preferably, the odour removal apparatus comprises an air extractor or activated carbon.

Preferably, in the step of heat-treating the garlic at a certain temperature for a period of time, the certain temperature is 58-62 ℃, and the period of time is 18-19 min.

Preferably, the separation apparatus comprises a centrifuge.

Preferably, the ratio of the garlic powder to the water body is 8: 100-14: 100, respectively.

Preferably, the enzymolysis solution is a mixed solution of protease and a water body.

Preferably, the alkali liquor is sodium hydroxide solution.

Compared with the prior art, the invention has the beneficial effects that:

(1) according to the invention, the odor is removed through the odor removing device, so that the odor in the garlic mixed solution is removed, the garlic mixed solution is prevented from generating the odor, the preparation of garlic proteolysis is not influenced, the preparation difficulty of proteolysis is reduced, and the preparation efficiency of proteolysis is improved.

(2) According to the invention, the protease and the water body are mixed in advance, so that an enzymolysis solution is formed by the protease and the water body, and the enzymolysis efficiency of the garlic mixed solution is accelerated by mixing the enzymolysis solution and the garlic mixed solution, thereby further reducing the preparation difficulty of the garlic protein zymolyte and further improving the preparation efficiency of the garlic protein zymolyte.

(3) According to the invention, the garlic enzymolysis solution is vibrated by the vibration equipment, so that the enzymolysis process of the garlic enzymolysis solution is accelerated, the preparation difficulty of the garlic enzymolysis solution is further reduced, and the preparation efficiency of the garlic enzymolysis solution is further improved.

Drawings

FIG. 1 is a flow chart of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1, the present invention provides the following technical solutions: a garlic protein zymolyte is a mixture of active polypeptide compounds containing 3-9 peptides and polysaccharide compounds, wherein the components and the weight percentage of dry matter are 71-78% of the content of the polypeptide compounds, 7-14% of the content of the polysaccharide compounds and the balance of amino acids.

A preparation method of garlic protein zymolyte comprises the following steps:

s1, crushing clean garlic to obtain garlic powder, mixing the garlic powder with a water body to form a garlic mixed solution, and placing the garlic mixed solution in a smell removal device for removing smell;

s2, adding an enzymolysis solution and alkali liquor into the deodorized garlic mixed solution, adjusting the pH value to 7.6-8.4, and carrying out enzymolysis on the deodorized garlic mixed solution to form a garlic enzymolysis solution;

s3, separating and filtering the garlic enzymolysis solution to form a liquid-phase enzymolysis substrate and a solid-phase enzymolysis substrate

Further, the method comprises the following steps of crushing clean garlic to obtain garlic powder, mixing the garlic powder with a water body to form a garlic mixed solution, and placing the garlic mixed solution in a smell removal device for smell removal:

s11, cleaning mud on the surface of the garlic, peeling the garlic and then cleaning the garlic so as to clean the garlic and obtain clean garlic;

s12, drying the clean garlic to remove water on the surface of the garlic and dry the garlic;

s13, carrying out heat treatment on the garlic for a period of time under a certain temperature condition;

s14, after the garlic is subjected to heat treatment, crushing the garlic through crushing equipment to obtain garlic powder;

s15, placing the garlic powder into a container, adding water into the container according to a certain proportion, and mixing the garlic powder and the water to form a garlic mixed solution.

Further, adding the enzymolysis solution and alkali liquor into the deodorized garlic mixed solution to carry out enzymolysis on the deodorized garlic mixed solution to form a garlic enzymolysis solution, and then, the method comprises the following steps:

s21, placing the garlic enzymolysis solution in a shaking device for shaking;

s22, separating the vibrated garlic enzymolysis solution by using separation equipment to obtain a primary liquid-phase enzymolysis substrate and a primary solid-phase enzymolysis substrate;

s23, filtering the primary liquid-phase enzymolysis substrate and the primary solid-phase enzymolysis substrate, so that the primary liquid-phase enzymolysis substrate and the primary solid-phase enzymolysis substrate are separated to obtain a liquid-phase enzymolysis substrate and a solid-phase enzymolysis substrate.

Further, the odor removal device comprises an air extractor or activated carbon.

Further, in the step of carrying out heat treatment on the garlic for a period of time under a certain temperature condition, the certain temperature condition is 58-62 ℃, and the period of time is 18-19 min.

Further, the separation device comprises a centrifuge.

Further, the ratio of the garlic powder to the water body is 8: 100-14: 100, respectively.

Further, the enzymolysis solution is a mixed solution of protease and a water body.

Further, the alkali liquor is sodium hydroxide solution.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.