阅读说明：本技术 一种优质高配合力籼粳交偏粳恢复系的选育方法 (Breeding method of high-quality high-combining-ability indica-japonica hybrid biased japonica restorer ) 是由 刘波 李晓元 肖光余 王明贵 于 2021-02-23 设计创作，主要内容包括：本发明提供了一种优质高配合力籼粳交偏粳恢复系的选育方法,用优质粳稻与优质高产抗病籼粳交杂交稻F1杂交得复交F1代；在F1代群体选择收种,得F2代；在F2代群体选择收种,得F3代；在F3代群体选择收种,得F4代；种植F4代与优质两系不育系测交,得测交F1代,在F4代群体选择收种,得F5代；种植测交F1代进行优势鉴定,选择强优势组合对应父本种植F5代并复测,得到复测F1代,在F5代群体选择收种,得F6代；种植复测F1优势再鉴定,选择强优势组合对应父本种植F6代,在F6代群体选择收种,得F7代,即得。本发明用市售高产抗病的籼粳交杂交稻品种与优质粳稻杂交选育优质籼粳交偏粳恢复系。(The invention provides a breeding method of a high-quality and high-combining ability indica-japonica hybrid cross-japonica restorer, which is characterized in that high-quality japonica rice and high-quality and high-yield disease-resistant indica-japonica hybrid rice F1 are hybridized to obtain a compound cross F1 generation; selecting and harvesting seeds in the F1 generation population to obtain an F2 generation; selecting and harvesting seeds in the F2 generation population to obtain an F3 generation; selecting and harvesting seeds in the F3 generation population to obtain an F4 generation; testing crossing the planted F4 generation with high-quality two-line sterile line to obtain testing crossing F1 generation, and selectively harvesting seeds in F4 generation to obtain F5 generation; performing dominance identification on the planting test cross F1 generation, selecting a strong dominance combination corresponding to the male parent to plant the F5 generation and retest to obtain a retest F1 generation, and selecting a seed harvest in the F5 generation population to obtain an F6 generation; and (4) re-testing the advantages of the F1, re-identifying the advantages, selecting a strong advantage combination corresponding to the male parent for planting F6 generations, and selecting seeds for harvest in F6 generation groups to obtain F7 generations. The invention relates to a method for breeding a high-quality indica-japonica hybrid japonica restorer by hybridizing a commercially available indica-japonica hybrid rice variety with high yield and disease resistance with high-quality japonica rice.)

1. A breeding method of a high-quality indica-japonica rice cross-over segregation-japonica rice restorer line with high combining ability is characterized by comprising the following steps:

s1, hybridizing high-quality japonica rice and high-quality and high-yield disease-resistant indica-japonica hybrid rice in subtropical regions in summer in the first year, and obtaining a compound hybrid F1 generation of japonica/indica-japonica hybrid rice F1 or indica-japonica hybrid rice F/japonica rice of the indica-japonica hybrid rice;

s2, planting compound cross F1 generation and high-quality japonica rice parent obtained in S1 in tropical area in winter of the year, selecting single plant with disease resistance, high seed setting rate and excellent agronomic character and different from parent to harvest seeds after F1 generation seeds are mature in spring of the next year to obtain F2 generation;

s3, after the F2 generation obtained in the S2 is subjected to rice quality screening, F2 generation is planted in subtropical zone in summer in the current year, after the seeds of the F2 generation are mature, single plants in a strain line with high disease resistance and seed setting rate and good agronomic characters are selected for seed collection, and an F3 generation is obtained;

s4, after the F3 generation obtained in the S3 is subjected to rice quality screening, in winter of the year, F3 generation is planted in the tropical additional generation, after the seeds of the F3 generation are mature, a single plant in a strain line with high disease resistance and seed setting rate and good agronomic characters is selected for seed collection, and an F4 generation is obtained;

s5, after the F4 generation obtained in the S4 is subjected to rice quality screening, planting F4 generation in subtropical zone in summer in the third year, and carrying out test cross on the excellent strains of the F4 generation group by using the high-quality and high-combining ability two-line sterile line to obtain a test cross F1 generation; after the seeds of the F4 generation are mature, selecting excellent single plants from the test cross strains for seed collection to obtain an F5 generation;

s6, planting test cross F1 generation and regional test contrast variety obtained in S5 in subtropical zone in summer of the fourth year, and performing advantage identification;

s7, according to the advantage identification test result of the test cross F1 generation in S6, planting the F5 generation obtained in S5 corresponding to the strong advantage combination in the test cross F1 generation in the tropical season in the winter of the year, and retesting the F5 generation plant line by using a high-combining ability high-quality sterile line to obtain retested F1 generation seeds; after the seeds of the F5 generation are mature, selecting excellent single plants from the retest strains to harvest seeds to obtain an F6 generation;

s8, planting the re-tested F1 generation and the regional test control variety obtained in S7 in subtropical regions in summer of the fifth year, and performing advantage re-identification;

s9, screening out a strong advantage combination in the re-testing F1 generation test according to the re-testing result of the advantages of the re-testing F1 generation in S8, planting the F6 generation in S7 corresponding to the strong advantage combination in the re-testing F1 generation in the tropical season in the winter of the year, harvesting seeds after the seeds of the F6 generation are mature, obtaining the F7 generation, and obtaining the indica-japonica segregation-japonica restoration line with high quality and high combining ability.

2. The breeding method of the indica-japonica hybrid japonica restorer line with high quality and high combining ability as claimed in claim 1, wherein the high quality japonica rice in S1 is Huai rice No. 5, Zhenxiang No. 24, Hu Xiang No. 2.

3. The breeding method of the indica-japonica hybrid segregation-japonica restorer line with high quality and high combining ability as claimed in claim 1, wherein the indica-japonica hybrid rice with high quality, high yield and disease resistance in S1 is Yongyou 1538, Yongyou 7872 and Jiayou Zhongke 13-1.

4. The breeding method of the indica-japonica hybrid segregation-japonica restorer line with high quality and high combining ability as claimed in claim 1, wherein the number of the plants of the compound F1 generation in S2 is more than or equal to 1000.

5. The breeding method of the indica-japonica hybrid segregation-japonica restorer line with high quality and high combining ability as claimed in claim 1, wherein the agronomic traits of S2-S4 are growth period, plant leaf morphology, plant height, spike type and grain type.

6. The breeding method of a high-quality high-combining ability indica-japonica hybrid segregation-japonica restorer line as claimed in claim 1, wherein the methods of superiority identification in S6 and reiteration of superiority in S8 are both yield and resistance identification.

7. The breeding method of a high-quality indica-japonica hybrid biased indica restorer line with high combining ability as claimed in claim 1, wherein the strong dominant combination selected by the dominant test in S7 and the dominant test in S9 is a cross test combination with the yield absolute value of more than or equal to 600kg and the yield increase of more than or equal to 10% compared with the highest yield value.

Technical Field

The invention belongs to the technical field of sexual cross breeding of rice, and particularly relates to a breeding method of a high-quality indica-japonica hybrid over-japonica restoring line with high combining ability.

Background

The grain problem is the first major concern of China's county. With the increase of population and the reduction of cultivated land, the grain safety pressure is getting bigger and bigger. The method increases the production potential of the variety by breeding means, and is an effective way for improving the yield per unit of rice by matching with corresponding cultivation technology. The utilization of excellent germplasm resources to obtain breakthrough breeding materials is the basis and precondition for breeding excellent varieties. The heterosis of indica-japonica subspecies is super strong, and the direct or indirect utilization of the heterosis of indica-japonica rice subspecies becomes one of the targets of super hybrid rice breeding. The indica-japonica hybrid rice varieties applied in large area in the current production, such as Yongyou series, spring-you series and Jiayou Zhongke series, are excellent varieties which are carefully cultivated and subjected to market inspection by breeders for years, and are typical indica-japonica hybrid varieties. Inside are a number of advantageous genes such as: the broad-affinity gene S5-n, the invisible semi-dwarf gene sd1, the ideal plant type gene IPA1, the dominant semi-dwarf gene Sdd, the Wxb gene and the like can be utilized. Therefore, the method takes indica-japonica hybrid rice as a basic material, expands the genetic basis of breeding materials, and is an effective way for creating new germplasm resources. Has important significance for improving the utilization efficiency of hybrid seeds and promoting rice breeding.

Disclosure of Invention

The method for breeding the high-quality high-combining ability indica-japonica hybrid segregation-japonica restorer line provided by the invention is characterized in that the high-quality japonica rice is hybridized with the commercially available high-quality high-yield disease-resistant indica-japonica hybrid rice F1 (japonica rice/indica-japonica hybrid rice F1 or indica-japonica hybrid rice F/japonica rice) to breed a new high-quality high-combining ability indica-japonica hybrid segregation-japonica restorer line.

The technical scheme adopted by the invention is as follows: a breeding method of a high-quality indica-japonica rice cross-over segregation-japonica rice restorer line with high combining ability comprises the following steps:

s1, in subtropical zone in summer in the first year, hybridizing high-quality japonica rice with commercially available high-quality high-yield disease-resistant indica-japonica hybrid rice (japonica/indica-japonica hybrid rice F1 or indica-japonica hybrid rice F/japonica rice) to obtain a compound hybrid F1 generation;

s2, planting compound cross F1 generation and high-quality japonica rice parent obtained in S1 in the tropical zone in the winter of the year, and harvesting a single plant which has high disease resistance and seed setting rate and excellent agronomic character and is different from the parent after the seeds of the F1 generation are mature in the spring of the second year to obtain the F2 generation;

s3, after the F2 generation obtained in the S2 is subjected to rice quality screening, F2 generation is planted in subtropical zone in summer in the current year, after the seeds of the F2 generation are mature, single plants in a strain line with high disease resistance and seed setting rate and good agronomic characters are selected for seed collection, and an F3 generation is obtained;

s4, after the F3 generation obtained in the S3 is subjected to rice quality screening, in winter of the year, F3 generation is planted in the tropical additional generation, after the seeds of the F3 generation are mature, a single plant in a strain line with high disease resistance and seed setting rate and good agronomic characters is selected for seed collection, and an F4 generation is obtained;

s5, after the F4 generation obtained in the S4 is subjected to rice quality screening, planting F4 generation in subtropical zone in summer in the third year, and carrying out test cross on the excellent strains of the F4 generation group by using the high-quality and high-combining ability two-line sterile line to obtain a test cross F1 generation; after the seeds of the F4 generation are mature, selecting excellent single plants from the test cross strains for seed collection to obtain an F5 generation;

s6, planting test cross F1 generation and regional test contrast variety obtained in S5 in subtropical zone in summer of the fourth year, and performing advantage identification;

s7, according to the advantage identification test result of the test cross F1 generation in S6, planting the F5 generation obtained in S5 corresponding to the strong advantage combination in the test cross F1 generation in the tropical season in the winter of the year, and retesting the F5 generation plant line by using a high-combining ability high-quality sterile line to obtain retested F1 generation seeds; after the seeds of the F5 generation are mature, selecting excellent single plants from the retest strains to harvest seeds to obtain an F6 generation;

s8, planting the re-tested F1 generation and the regional test control variety obtained in S7 in subtropical regions in summer of the fifth year, and performing advantage re-identification;

s9, screening out a strong advantage combination in the re-testing F1 generation test according to the re-testing result of the advantages of the re-testing F1 generation in S8, planting the F6 generation in S7 corresponding to the strong advantage combination in the re-testing F1 generation in the tropical season in the winter of the year, harvesting seeds after the seeds of the F6 generation are mature, obtaining the F7 generation, and obtaining the indica-japonica segregation-japonica restoration line with high quality and high combining ability.

The high-quality japonica rice in S1 is Huai rice No. 5, Zhenxiang No. 24 and Hubei rice No. 2.

The high-quality, high-yield and disease-resistant indica-japonica hybrid rice in S1 is Yongyou 1538, Yongyou 7872 and Jiayou Zhongke 13-1.

The number of the plants of the compound cross F1 generation in S2 is more than or equal to 1000.

The agronomic traits of S2-S4 are growth period, leaf morphology, plant height, spike shape and grain shape.

The methods of superiority identification in S6 and superiority re-identification in S8 were both yield and resistance identification.

The strong advantage combination screened by the advantage identification in S7 and the advantage identification in S9 is a test cross combination with the yield absolute value of more than or equal to 600kg and the yield increased by more than or equal to 10 percent compared with the highest yield value.

Compared with the prior art, the invention has the following advantages:

the method utilizes the commercial indica-japonica hybrid rice with high quality, high yield and disease resistance to breed the new rice restorer with high quality and high combining ability by hybridizing the indica-japonica hybrid rice with high quality and japonica rice (japonica rice/indica-japonica hybrid rice F1 or indica-japonica hybrid rice F/japonica rice), breaks the blockade of breeders on breeding materials to a certain extent, expands the genetic basis of the breeding materials, improves the breeding efficiency, is expected to breed the rice restorer with good resistance and high quality, and further prepares a new rice variety with high quality, disease resistance and high yield.

Detailed Description

The present invention will be described in further detail with reference to examples.

The embodiment of the invention relates to a breeding method of a high-quality high-combining-ability indica-japonica hybrid segregation-japonica restorer, which comprises the following steps:

s1, in the river oil city of Sichuan province in summer in the first year, hybridizing the high-quality japonica rice No. 5 with the commercially available high-quality high-yield disease-resistant indica-japonica hybrid rice Yongyou 1538 (Huai rice No. 5/Yongyou 1538 or Yongyou 1538/Huai rice No. 5) to obtain a compound cross F1 generation;

s2, planting compound cross F1 and high-quality japonica rice parent Huai rice No. 5 in Suiyan province of Suiyan province S1 in winter in the current year, and harvesting a single plant which is resistant to diseases, high in maturing rate, excellent in agronomic characters and different from the parent Huai rice No. 5 after seeds of the F1 generation are mature in spring of the second year to obtain F2 generation;

s3, after the F2 generation obtained in the S2 is subjected to rice quality screening, F2 generation is planted in oil city in the river in summer next year, and after the seeds of the F2 generation are mature, single plants in a strain line with high disease resistance and seed setting rate and excellent agronomic characters are selected for seed collection to obtain F3 generation;

s4, after the F3 generation obtained in the S3 is subjected to rice quality screening, in winter of the year, F3 generation is planted in the south of Hainan, after the seeds of the F3 generation are mature, single plants in a strain with high disease resistance and seed setting rate and good agronomic characters are selected for seed collection, and F4 generation is obtained;

s5, after the F4 generation obtained in S4 is subjected to rice quality screening, F4 generation is planted in oil city in the river in summer in the third year, and excellent plant lines of F4 generation group are subjected to test cross by using high-quality high-combining ability two-line sterile lines C815S, Y58S, N5088S and 7001S to obtain a test cross F1 generation; after the seeds of the F4 generation are mature, selecting excellent single plants from the test cross strains for seed collection to obtain an F5 generation;

s6, planting test cross F1 generation and upstream region test contrast varieties F you 498 and Yixiangyou 2115 obtained in S5 in the oil city of the river in summer of the fourth year, and performing advantage identification;

s7, according to the advantage identification test result of the test cross F1 generation in S6, planting the F5 generation obtained in S5 corresponding to the strong advantage combination in the test cross F1 generation in Hainan in winter in the current year, and performing retest on F5 generation plant lines by using high-quality and high-combining-ability two-line sterile lines C815S, Y58S, N5088S and 7001S to obtain retest F1 generation seeds; after the seeds of the F5 generation are mature, selecting excellent single plants from the retest strains to harvest seeds to obtain an F6 generation;

s8, retesting F1 generation obtained in the oil planting S7 in summer of the fifth year, and testing the control varieties F you 498 and Yixiangyou 2115 in the upstream area for advantage re-identification;

s9, screening out a strong advantage combination in the repeated F1 generation test according to the re-identified test result of the advantages of the repeated F1 generation in S8, planting an F6 generation obtained in S7 corresponding to the strong advantage combination in the repeated F1 generation in Hainan in winter in the current year, harvesting seeds after the seeds of the F6 generation are mature, and obtaining an F7 generation to obtain the high-quality indica-japonica segregation-japonica restoration line with high combining ability.

In the embodiment, a new high-quality high-combining-ability indica-japonica hybrid segregation restorer line is bred by utilizing commercially available indica-japonica hybrid rice Yongyou 1538 with high-quality japonica rice Huai-rice No. 5 hybrid (Huai-rice No. 5/Yongyou 1538, Yongyou 1538/Huai-rice No. 5), so that the blockade of breeding families on breeding materials is broken to a certain extent, the genetic basis of the breeding materials is expanded, and the breeding efficiency is improved.

The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention in any way. Any simple modification, change and equivalent changes of the above embodiments according to the technical essence of the invention are still within the protection scope of the technical solution of the invention.