阅读说明：本技术 一种铁皮石斛种植种苗预处理方法 (Dendrobium officinale planting seedling pretreatment method ) 是由 钱明舟 于 2020-12-01 设计创作，主要内容包括：本发明涉及铁片石斛种植技术领域,具体为一种铁皮石斛种植种苗预处理方法,包括吲哚乙酸和萘乙酸钠,具体步骤为：从铁皮石斛植株上剪取,放入酒精中进行消毒,将其以茎节为中心切成小段,浸泡后除水,用戊二醛水溶液进行循环冲洗,将腋芽苞叶切除,再次用戊二醛水溶液进行循环冲洗并除水,将处理后的铁皮石斛种苗分别放入到培养基,加入分化水溶液,将铁皮石斛幼苗放入到生根培养瓶中进行培养,生根水溶液,最后将铁皮石斛幼苗清洗烘干并放入驯化培养基中。通过对比实施例一到三,当使用水培以及加入不同含量比例的吲哚乙酸和萘乙酸钠可以对幼苗的生根率以及生根效果进行提升,间接地增加了幼苗在后续种植中的存活率。(The invention relates to the technical field of dendrobium officinale planting, in particular to a method for pretreating dendrobium officinale planting seedlings, which comprises indoleacetic acid and sodium naphthaleneacetate, and comprises the following steps: cutting dendrobium officinale plants, putting the dendrobium officinale plants into alcohol for disinfection, cutting the dendrobium officinale plants into small segments by taking stem nodes as centers, soaking the small segments, removing water, circularly washing the small segments with glutaraldehyde aqueous solution, cutting axillary buds, circularly washing the small segments with glutaraldehyde aqueous solution again and removing water, respectively putting the treated dendrobium officinale seedlings into a culture medium, adding a differentiation aqueous solution, putting the dendrobium officinale seedlings into a rooting culture bottle for culture, rooting the aqueous solution, and finally cleaning, drying and putting the dendrobium officinale seedlings into a domestication culture medium. Through comparison of the first to the third examples, when water culture is used and indoleacetic acid and sodium naphthaleneacetate with different content ratios are added, the rooting rate and rooting effect of the seedlings can be improved, and the survival rate of the seedlings in subsequent planting is indirectly increased.)

1. A pretreatment method for dendrobium officinale planting seedlings comprises indoleacetic acid and sodium naphthaleneacetate, and comprises the following specific steps:

the method comprises the following steps of firstly, shearing dendrobium officinale plants to obtain dendrobium officinale seedlings, and putting the dendrobium officinale seedlings into alcohol with the concentration of 60-75% for sterilization and disinfection;

step two, cutting the disinfected dendrobium officinale seedlings into small sections by taking stem nodes as centers, keeping the reserved length of the upper end and the lower end of each stem node to be 0.5-0.7 cm, soaking the small sections in water for 5-10 min, and completely absorbing the surface moisture of the small sections by using sterile tissue paper;

circularly washing the dendrobium officinale seedlings with an aqueous solution with the glutaraldehyde content of 8-10%, throwing off axillary buds of the dendrobium officinale seedlings by using a cutter, circularly washing the dendrobium officinale seedlings with an aqueous solution with the glutaraldehyde content of 8-10% again, and completely absorbing surface moisture of the dendrobium officinale seedlings by using sterile tissue paper again;

step four, respectively putting the treated dendrobium officinale seedlings into a culture medium to be cultured for three weeks at the temperature of 20-28 ℃, and finally obtaining the dendrobium officinale seedlings with callus;

step five, adding an aqueous solution containing coconut juice, yeast extract and malt extract into the culture medium to obtain dendrobium officinale seedlings;

putting the dendrobium officinale seedlings into a rooting culture bottle for culture, fixing two ends of the dendrobium officinale, adding a rooting aqueous solution containing amino acid, potassium, calcium, alcohol, white sugar, indoleacetic acid, sodium naphthylacetate and vitamin substances, wherein the proportion of the indoleacetic acid in the aqueous solution is 30-40%, the proportion of the sodium naphthylacetate in the aqueous solution is 50-55%, and the time for soaking the dendrobium officinale seedlings is 10-20 min;

seventhly, taking out the dendrobium officinale seedlings from the rooting aqueous solution, washing the dendrobium officinale seedlings by using 70-60% of alcohol, then washing the dendrobium officinale seedlings for the second time by using distilled water, and completely absorbing the surface water of the dendrobium officinale seedlings by using sterile tissue paper;

and step eight, putting the dendrobium officinale seedlings into a domestication culture medium for culturing, keeping the domestication environment the same as the actual planting environment, and culturing for 10-15 days.

2. The pretreatment method of dendrobium officinale planting seedlings according to claim 1, which is characterized in that: and the water used in the second step is distilled water, and the water temperature is 5-10 ℃.

3. The pretreatment method of dendrobium officinale planting seedlings according to claim 1, which is characterized in that: the planting environment selected in the third step is 70-75% of air relative humidity and 3000-3500 lx of illumination.

4. The pretreatment method of Dendrobium officinale Kimura et Migo seedlings according to claim 1, wherein the pretreatment method comprises the following steps: and the washing time of the aqueous solution with the glutaraldehyde content of 8-10% used twice in the fourth step is 10-15 min.

5. The pretreatment method of dendrobium officinale planting seedlings according to claim 1, which is characterized in that: the knife used in the fourth step is a disinfection knife soaked by liquid, and the used disinfection water solution contains alcohol and formaldehyde, and the specific gravity of the alcohol and the formaldehyde is 7: 3.

6. The pretreatment method of dendrobium officinale planting seedlings according to claim 1, which is characterized in that: the contents of the coconut juice, the yeast extract and the malt extract in the differentiation aqueous solution used in the fifth step are 35%, 35% and 30% respectively.

7. The pretreatment method of dendrobium officinale planting seedlings according to claim 1, which is characterized in that: and in the sixth step, the illumination time of the planting environment of the culture medium is not less than 10h every day.

Technical Field

The invention relates to the technical field of dendrobium officinale, in particular to a pretreatment method for dendrobium officinale planting seedlings.

Background

Dendrobium officinale: the stem is upright, cylindrical, 9-35 cm long, 2-4 mm thick, does not branch and has multiple sections; the second row of leaves, paper, long round and needle-shaped, the edge and middle rib are often purplish. The raceme usually comes out from the upper part of the old stem with fallen leaves and has 2-3 flowers; the buds are dry, light white, oval, 5-7 mm long, the sepals and the petals are yellow green and similar, the long round shape is coated with needles, the lips are white, the base parts are provided with 1 green or yellow callus, the egg shape is coated with needles, the length is slightly shorter than the sepals, and the middle part is reversely folded.

The dendrobium officinale has the effects of diminishing inflammation, removing heat, nourishing yin, promoting the production of body fluid, tonifying spleen and stomach and the like, and has very strong medicinal value. The growth conditions of wild dendrobium officinale are extremely harsh, people begin to carry out artificial cultivation for realizing mass production, the most key step in the current artificial cultivation is the treatment of the planting seedlings of the dendrobium officinale, the current planting seedlings can be roughly divided into intercepting branches and stems for seed transfer and seed bottle preparation, wherein the intercepting branches can more quickly obtain the planting seedlings of the dendrobium officinale, but the rooting is slow in the follow-up process of acclimating the seedlings, so that the seedlings are easy to blacken, lesion and even rot, and the death of the seedlings is finally caused, and therefore a pretreatment method for the dendrobium officinale planting seedlings needs to be designed.

Disclosure of Invention

The invention aims to provide a pretreatment method for dendrobium officinale planting seedlings, which aims to solve the problem that the existing planting seedlings proposed in the background technology can be roughly divided into an intercepting branch and a seed bottle for seed transformation and seed bottle matching, wherein the intercepting branch can more quickly acquire the planting seedlings of the dendrobium officinale, but the rooting is slow in the follow-up process of acclimatization of the seedlings, so that the seedlings are easy to blacken, lesion and even rot, and finally the seedlings die.

In order to achieve the purpose, the invention provides the following technical scheme: a pretreatment method for dendrobium officinale planting seedlings comprises indoleacetic acid and sodium naphthaleneacetate, and comprises the following specific steps:

the method comprises the following steps of firstly, shearing dendrobium officinale plants to obtain dendrobium officinale seedlings, and putting the dendrobium officinale seedlings into alcohol with the concentration of 60-75% for sterilization and disinfection;

step two, cutting the disinfected dendrobium officinale seedlings into small sections by taking stem nodes as centers, keeping the reserved length of the upper end and the lower end of each stem node to be 0.5-0.7 cm, placing the small sections in water for soaking for 5-10 min, and completely absorbing the surface moisture of the small sections by using sterile tissue paper;

circularly washing the dendrobium officinale seedlings with an aqueous solution with the glutaraldehyde content of 8-10%, throwing off axillary buds of the dendrobium officinale seedlings by using a cutter, circularly washing the dendrobium officinale seedlings with an aqueous solution with the glutaraldehyde content of 8-10% again, and completely absorbing surface moisture of the dendrobium officinale seedlings by using sterile tissue paper again;

step four, respectively putting the treated dendrobium officinale seedlings into a culture medium to be cultured for three weeks at the temperature of 20-28 ℃, and finally obtaining the dendrobium officinale seedlings with callus;

step five, adding an aqueous solution containing coconut juice, yeast extract and malt extract into the culture medium to obtain dendrobium officinale seedlings;

putting the dendrobium officinale seedlings into a rooting culture bottle for culture, fixing two ends of the dendrobium officinale, adding a rooting aqueous solution containing amino acid, potassium, calcium, alcohol, white sugar, indoleacetic acid, sodium naphthylacetate and vitamin substances, wherein the proportion of the indoleacetic acid in the aqueous solution is 30-40%, the proportion of the sodium naphthylacetate in the aqueous solution is 50-55%, and the time for soaking the dendrobium officinale seedlings is 10-20 min;

seventhly, taking out the dendrobium officinale seedlings from the rooting aqueous solution, washing the dendrobium officinale seedlings by using 70-60% of alcohol, then washing the dendrobium officinale seedlings for the second time by using distilled water, and completely absorbing the surface water of the dendrobium officinale seedlings by using sterile tissue paper;

and step eight, putting the dendrobium officinale seedlings into a domestication culture medium for culturing, keeping the domestication environment the same as the actual planting environment, and culturing for 10-15 days.

Preferably, the water used in the second step is distilled water, and the water temperature is 5-10 ℃.

Preferably, the planting environment selected in the third step is 70-75% of air relative humidity and 3000-3500 lx of illumination.

Preferably, the washing time of the aqueous solution with the glutaraldehyde content of 8-10% used twice in the fourth step is 10-15 min.

Preferably, the knife used in the fourth step is a sterilized knife soaked in liquid, and the used sterilized water solution contains alcohol and formaldehyde, and the specific gravity of the alcohol and the formaldehyde is 7: 3.

Preferably, the differentiation aqueous solution used in the fifth step has a coconut juice content of 35%, a yeast extract content of 35%, a malt extract content of 30%, and a malt extract content of 35%.

Preferably, the planting environment of the culture medium in the sixth step is not less than 10 hours per day.

Compared with the prior art, the invention has the beneficial effects that: through comparison examples one to three, when using water planting and adding different content proportions's indoleacetic acid and sodium naphthylacetate can promote the rooting rate and the effect of taking root of seedling, increased the survival rate of seedling in subsequent planting indirectly, the root rot condition can reduce the pollution effect to the soil when seedling planting when reducing the seedling planting simultaneously.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

A pretreatment method for dendrobium officinale planting seedlings comprises indoleacetic acid and sodium naphthaleneacetate, and comprises the following specific steps:

the method comprises the following steps of firstly, shearing dendrobium officinale plants to obtain dendrobium officinale seedlings, and putting the dendrobium officinale seedlings into alcohol with the concentration of 60-75% for sterilization and disinfection;

step two, cutting the disinfected dendrobium officinale seedlings into small sections by taking stem nodes as centers, keeping the reserved length of the upper end and the lower end of each stem node to be 0.5-0.7 cm, placing the small sections in water for soaking for 5-10 min, and completely absorbing the surface moisture of the small sections by using sterile tissue paper;

circularly washing the dendrobium officinale seedlings with an aqueous solution with the glutaraldehyde content of 8-10%, throwing off axillary buds of the dendrobium officinale seedlings by using a cutter, circularly washing the dendrobium officinale seedlings with an aqueous solution with the glutaraldehyde content of 8-10% again, and completely absorbing surface moisture of the dendrobium officinale seedlings by using sterile tissue paper again;

step four, respectively putting the treated dendrobium officinale seedlings into a culture medium to be cultured for three weeks at the temperature of 20-28 ℃, and finally obtaining the dendrobium officinale seedlings with callus;

step five, adding an aqueous solution containing coconut juice, yeast extract and malt extract into the culture medium to obtain dendrobium officinale seedlings;

putting the dendrobium officinale seedlings into a rooting culture bottle for culture, fixing two ends of the dendrobium officinale, adding a rooting aqueous solution containing amino acid, potassium, calcium, alcohol, white sugar, indoleacetic acid, sodium naphthylacetate and vitamin substances, wherein the proportion of the indoleacetic acid in the aqueous solution is 30-40%, the proportion of the sodium naphthylacetate in the aqueous solution is 50-55%, and the time for soaking the dendrobium officinale seedlings is 10-20 min;

seventhly, taking out the dendrobium officinale seedlings from the rooting aqueous solution, washing the dendrobium officinale seedlings by using 70-60% of alcohol, then washing the dendrobium officinale seedlings for the second time by using distilled water, and completely absorbing the surface water of the dendrobium officinale seedlings by using sterile tissue paper;

and step eight, putting the dendrobium officinale seedlings into a domestication culture medium for culturing, keeping the domestication environment the same as the actual planting environment, and culturing for 10-15 days.

Further, the water used in the second step is distilled water, and the water temperature is 5-10 ℃.

Furthermore, the planting environment selected in the third step is 70-75% of air relative humidity and 3000-3500 lx of illumination.

Furthermore, the washing time of the aqueous solution with the glutaraldehyde content of 8-10% used in the fourth step is 10-15 min.

Further, the knife used in the fourth step is a disinfection knife soaked by liquid, and the used disinfection water solution contains alcohol and formaldehyde, wherein the specific gravity of the alcohol and the formaldehyde is 7: 3.

Further, the content of coconut juice, yeast extract and malt extract in the aqueous differentiation solution used in the fifth step is 35%, 35% and 30%, respectively.

Further, the illumination time of the planting environment of the culture medium in the sixth step is not less than 10 hours per day.

The first embodiment;

the method comprises the following steps of firstly, shearing dendrobium officinale plants to obtain dendrobium officinale seedlings, and putting the dendrobium officinale seedlings into 60% alcohol for sterilization and disinfection;

step two, cutting the disinfected dendrobium officinale seedlings into small sections by taking the stem nodes as the center, keeping the reserved length of the upper end and the lower end of each stem node to be 0.7cm, placing the small sections in water for soaking for 5-10 min, and completely absorbing the surface moisture of the small sections by using sterile tissue paper;

step three, circularly washing the dendrobium officinale seedlings with an aqueous solution with the glutaraldehyde content of 10%, throwing off axillary buds of the dendrobium officinale seedlings by using a cutter, circularly washing the dendrobium officinale seedlings with the aqueous solution with the glutaraldehyde content of 10% again, and completely absorbing surface moisture of the dendrobium officinale seedlings with sterile tissue paper again;

step four, respectively putting the treated dendrobium officinale seedlings into a culture medium to be cultured for three weeks at 25 ℃, and finally obtaining the dendrobium officinale seedlings with callus;

step five, adding an aqueous solution containing coconut juice, yeast extract and malt extract into the culture medium to obtain dendrobium officinale seedlings;

putting the dendrobium officinale seedlings into a rooting culture bottle for culture, fixing two ends of the dendrobium officinale, adding a rooting aqueous solution containing amino acid, potassium, calcium, alcohol, white sugar, indoleacetic acid, sodium naphthylacetate and vitamin substances, wherein the proportion of the indoleacetic acid in the aqueous solution is 30%, the proportion of the sodium naphthylacetate in the aqueous solution is 50%, and the soaking time of the dendrobium officinale seedlings is 10-20 min;

seventhly, taking out the dendrobium officinale seedlings from the rooting aqueous solution, washing the dendrobium officinale seedlings by using 60% alcohol, then washing the dendrobium officinale seedlings for the second time by using distilled water, and completely absorbing the surface water of the dendrobium officinale seedlings by using sterile tissue paper;

and step eight, putting the dendrobium officinale seedlings into a domestication culture medium for culturing, keeping the domestication environment the same as the actual planting environment, and culturing for 10-15 days.

Taking out the dendrobium officinale seedlings from the circulating culture medium, and observing that the black root rate of each hundred plants is 15% and the rotten root rate is 12%.

Example two;

the method comprises the following steps of firstly, shearing dendrobium officinale plants to obtain dendrobium officinale seedlings, and putting the dendrobium officinale seedlings into 60% alcohol for sterilization and disinfection;

step two, cutting the disinfected dendrobium officinale seedlings into small sections by taking the stem nodes as the center, keeping the reserved length of the upper end and the lower end of each stem node to be 0.7cm, placing the small sections in water for soaking for 5-10 min, and completely absorbing the surface moisture of the small sections by using sterile tissue paper;

step three, circularly washing the dendrobium officinale seedlings with an aqueous solution with the glutaraldehyde content of 10%, throwing off axillary buds of the dendrobium officinale seedlings by using a cutter, circularly washing the dendrobium officinale seedlings with the aqueous solution with the glutaraldehyde content of 10% again, and completely absorbing surface moisture of the dendrobium officinale seedlings with sterile tissue paper again;

step four, respectively putting the treated dendrobium officinale seedlings into a culture medium to be cultured for three weeks at 25 ℃, and finally obtaining the dendrobium officinale seedlings with callus;

step five, adding an aqueous solution containing coconut juice, yeast extract and malt extract into the culture medium to obtain dendrobium officinale seedlings;

putting the dendrobium officinale seedlings into a rooting culture bottle for culture, fixing two ends of the dendrobium officinale, adding a rooting aqueous solution containing amino acid, potassium, calcium, alcohol, white sugar, indoleacetic acid, sodium naphthylacetate and vitamin substances, wherein the proportion of the indoleacetic acid in the aqueous solution is 35%, the proportion of the sodium naphthylacetate in the aqueous solution is 55%, and the time for soaking the dendrobium officinale seedlings is 10-20 min;

seventhly, taking out the dendrobium officinale seedlings from the rooting aqueous solution, washing the dendrobium officinale seedlings by using 60% alcohol, then washing the dendrobium officinale seedlings for the second time by using distilled water, and completely absorbing the surface water of the dendrobium officinale seedlings by using sterile tissue paper;

and step eight, putting the dendrobium officinale seedlings into a domestication culture medium for culturing, keeping the domestication environment the same as the actual planting environment, and culturing for 10-15 days.

And taking out the dendrobium officinale seedlings from the circulating culture medium, and observing that the black root rate of each hundred plants is 9 percent and the rotten root rate is 8 percent.

Example three;

the method comprises the following steps of firstly, shearing dendrobium officinale plants to obtain dendrobium officinale seedlings, and putting the dendrobium officinale seedlings into 60% alcohol for sterilization and disinfection;

step two, cutting the disinfected dendrobium officinale seedlings into small sections by taking stem nodes as centers, keeping the reserved length of the upper end and the lower end of each stem node to be 0.7cm, placing the small sections in water for soaking for 5-10 min, and completely absorbing the surface moisture of the small sections by using sterile tissue paper;

step three, circularly washing the dendrobium officinale seedlings with an aqueous solution with the glutaraldehyde content of 10%, throwing off axillary buds of the dendrobium officinale seedlings by using a cutter, circularly washing the dendrobium officinale seedlings with the aqueous solution with the glutaraldehyde content of 10% again, and completely absorbing surface moisture of the dendrobium officinale seedlings with sterile tissue paper again;

step four, respectively putting the treated dendrobium officinale seedlings into a culture medium to be cultured for three weeks at 25 ℃, and finally obtaining the dendrobium officinale seedlings with callus;

step five, adding an aqueous solution containing coconut juice, yeast extract and malt extract into the culture medium to obtain dendrobium officinale seedlings;

putting the dendrobium officinale seedlings into a rooting culture bottle for culture, fixing two ends of the dendrobium officinale, adding a rooting aqueous solution containing amino acid, potassium, calcium, alcohol, white sugar, indoleacetic acid, sodium naphthylacetate and vitamin substances, wherein the proportion of the indoleacetic acid in the aqueous solution is 40%, the proportion of the sodium naphthylacetate in the aqueous solution is 55%, and the time for soaking the dendrobium officinale seedlings is 10-20 min;

seventhly, taking out the dendrobium officinale seedlings from the rooting aqueous solution, washing the dendrobium officinale seedlings by using 60% alcohol, then washing the dendrobium officinale seedlings for the second time by using distilled water, and completely absorbing the surface water of the dendrobium officinale seedlings by using sterile tissue paper;

and step eight, putting the dendrobium officinale seedlings into a domestication culture medium for culturing, keeping the domestication environment the same as the actual planting environment, and culturing for 10-15 days.

And taking out the dendrobium officinale seedlings from the circulating culture medium, and observing that the black root rate of each hundred plants is 10% and the rotten root rate is 9%.

The first to third comparative examples are as follows: when the water culture is used and the indoleacetic acid and the sodium naphthaleneacetate with different content proportions are added, the rooting rate and the rooting effect of the seedling can be improved, the survival rate of the seedling in the subsequent planting is indirectly increased, meanwhile, the root rot condition in the seedling planting process is reduced, and the pollution effect of the seedling to the land in the planting process can be reduced.

Comparison was made by comparing the data from example one and example two, example two and three: the content of the indoleacetic acid and the sodium naphthaleneacetate in the hydroponic solution can affect the root lesion rate of the seedlings, but the influence on the lesion rate is gradually reduced along with the increasing of the content of the indoleacetic acid and the sodium naphthaleneacetate.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.