阅读说明：本技术 一种基于rt-qpcr的结直肠癌早期预警方法 (Colorectal cancer early warning method based on RT-QPCR ) 是由 凌雪峰 于 2021-01-20 设计创作，主要内容包括：本发明公开了一种基于RT-QPCR的结直肠癌早期预警方法,其方法包括如下步骤,血液抽取：首先将患有结直肠癌疾病人群的个人信息记录下来,同时抽取患者人群的血液制成样本A并进行保存；筛选高危人群：从当地人社局数据库中调取用户籍信息进行匹配,将患有结直肠癌疾病人群的直系后代的个人信息记录下来；本发明能够为高危人群提供有效的检测和预警,并使高危人群提前注意自身存在的风险,解决了目前由于直肠癌存有一定的遗传概率,导致直肠癌患者的直系后代为患病高危人群,且目前的预警方法存在着检测效率低下的缺陷,不能尽早发现直肠癌的患病风险,进而造成这些高危人群无法得到有效的检测和预警,病情容易延误的问题。(The invention discloses an early colorectal cancer early warning method based on RT-QPCR, which comprises the following steps of: firstly, recording personal information of a crowd with colorectal cancer diseases, and simultaneously extracting blood of the crowd of patients to prepare a sample A and storing the sample A; screening high risk groups: calling user registration information from a local community database for matching, and recording personal information of direct offspring of people with colorectal cancer diseases; the invention can provide effective detection and early warning for high-risk people, and enables the high-risk people to pay attention to risks in advance, thereby solving the problems that the rectal cancer patients are directly and later ill high-risk people due to certain genetic probability of the rectal cancer, the current early warning method has the defect of low detection efficiency, the ill risks of the rectal cancer cannot be found as early as possible, the high-risk people cannot be effectively detected and early warned, and the illness state is easily delayed.)

1. An early colorectal cancer early warning method based on RT-QPCR is characterized in that: the method comprises the following steps:

(1) blood drawing: firstly, recording personal information of a crowd with colorectal cancer diseases, and simultaneously extracting blood of the crowd of patients to prepare a sample A and storing the sample A;

(2) screening high risk groups: calling user registration information from a local community database for matching, and recording personal information of direct offspring of people with colorectal cancer diseases;

(3) high risk group detection: informing the high-risk people to go to a hospital for physical examination, if the early symptoms of the rectal cancer exist, immediately treating, and if the early symptoms of the rectal cancer do not exist, extracting blood to prepare a sample B and storing the sample B;

(4) gene extraction: taking a sample A of a patient and a sample B of a direct offspring of the patient, and then extracting and storing RNA of the sample A and the sample B;

(5) gene detection: performing gene testing on RNA extracted from the sample A and the sample B by using an RT-QPCR technology, comparing the susceptible conditions of the samples, wherein if the sample B is easy to infect, the probability of illness of the sample corresponding to a detection person is higher, and if the sample B is not easy to infect or does not infect, the probability of illness of the sample corresponding to the detection person is lower;

(6) and (3) carrying out crowd statistics: the personal information of the crowd with higher prevalence rate is intensively counted and made into a file A, and the personal information of the crowd with lower prevalence rate is intensively counted and made into a file B;

(7) and (4) notification and reinspection: and informing the crowd in the file A of the detection condition, informing the crowd in the file B of the detection condition at regular intervals, informing the crowd in the file B of the detection condition at irregular intervals to go to the hospital for recheck, and recording and storing recheck information of different crowds.

2. The early warning method for colorectal cancer based on RT-QPCR according to claim 1, wherein the early warning method comprises the following steps: in the step (1), the recorded personal information includes name, address, contact information and medical history.

3. The early warning method for colorectal cancer based on RT-QPCR according to claim 1, wherein the early warning method comprises the following steps: in the step (1), the blood is subjected to anticoagulation treatment after being drawn.

4. The early warning method for colorectal cancer based on RT-QPCR according to claim 1, wherein the early warning method comprises the following steps: in the step (3), the examination items of the high risk group include digital rectal examination, electronic colonoscopy, fecal occult blood test, CEA examination and CT scanning examination.

5. The early warning method for colorectal cancer based on RT-QPCR according to claim 1, wherein the early warning method comprises the following steps: and (3) in the step (3), the high risk group is informed of the personal information of other people without revealing.

6. The early warning method for colorectal cancer based on RT-QPCR according to claim 1, wherein the early warning method comprises the following steps: in the step (4), the extracted RNA is stored in an environment at-80 ℃ before gene detection.

7. The early warning method for colorectal cancer based on RT-QPCR according to claim 1, wherein the early warning method comprises the following steps: in the step (4), the personal information corresponding to the sample is repeatedly compared and confirmed in the process of taking out the sample.

8. The early warning method for colorectal cancer based on RT-QPCR according to claim 1, wherein the early warning method comprises the following steps: in the step (5), it is confirmed whether or not the samples a and B are contaminated before the detection.

9. The early warning method for colorectal cancer based on RT-QPCR according to claim 1, wherein the early warning method comprises the following steps: in the step (6), the files A and B are classified and stored after being prepared.

10. The early warning method for colorectal cancer based on RT-QPCR according to claim 1, wherein the early warning method comprises the following steps: in the step (7), the frequency of the retest of the archive A population is once in three months or half a year.

Technical Field

The invention relates to the technical field of medical treatment, in particular to an early colorectal cancer early warning method based on RT-QPCR.

Background

Rectal cancer refers to cancer from the dentate line to the rectosigmoid junction, and is one of the most common malignancies of the digestive tract. In China, the incidence and mortality of colorectal cancer are 5 th of malignant tumors, the incidence of colorectal cancer is in the rising stage, more than 40 million colorectal cancer patients are treated in China every year, about 20 million people die of colorectal cancer every year, and the incidence rate is increased by 4.2% in China and is higher than 2% of international level every year. The colorectal cancer has a slow natural disease course and definite precancerous lesions, is easy to screen, can improve and effectively reduce the incidence rate and the death rate of colorectal cancer by early screening, and has great significance in selecting colorectal cancer screening technical schemes due to the differences of colorectal cancer epidemiological characteristics, social, economic and cultural development and health care systems in different countries.

The colorectal cancer has a certain genetic probability, so that rectal cancer patients are directly behind high-risk groups, the existing early warning method has the defect of low detection efficiency, the risk of the rectal cancer cannot be found as early as possible, the high-risk groups cannot be effectively detected and warned, the group is easy to ignore the state of the disease, the state of the disease is delayed, and therefore the early warning method for the colorectal cancer, which can provide effective detection and early warning for the high-risk groups, enables the high-risk groups to pay attention to the risks in advance and has higher efficiency, is provided to solve the problem.

Disclosure of Invention

The invention aims to provide an early colorectal cancer early warning method based on RT-QPCR, so as to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: an early colorectal cancer early warning method based on RT-QPCR, which comprises the following steps:

(1) blood drawing: firstly, recording personal information of a crowd with colorectal cancer diseases, and simultaneously extracting blood of the crowd of patients to prepare a sample A and storing the sample A;

(2) screening high risk groups: calling user registration information from a local community database for matching, and recording personal information of direct offspring of people with colorectal cancer diseases;

(3) high risk group detection: informing the high-risk people to go to a hospital for physical examination, if the early symptoms of the rectal cancer exist, immediately treating, and if the early symptoms of the rectal cancer do not exist, extracting blood to prepare a sample B and storing the sample B;

(4) gene extraction: taking a sample A of a patient and a sample B of a direct offspring of the patient, and then extracting and storing RNA of the sample A and the sample B;

(5) gene detection: performing gene testing on RNA extracted from the sample A and the sample B by using an RT-QPCR technology, comparing the susceptible conditions of the samples, wherein if the sample B is easy to infect, the probability of illness of the sample corresponding to a detection person is higher, and if the sample B is not easy to infect or does not infect, the probability of illness of the sample corresponding to the detection person is lower;

(6) and (3) carrying out crowd statistics: the personal information of the crowd with higher prevalence rate is intensively counted and made into a file A, and the personal information of the crowd with lower prevalence rate is intensively counted and made into a file B;

(7) and (4) notification and reinspection: and informing the crowd in the file A of the detection condition, informing the crowd in the file B of the detection condition at regular intervals, informing the crowd in the file B of the detection condition at irregular intervals to go to the hospital for recheck, and recording and storing recheck information of different crowds.

Preferably, in step (1), the recorded personal information includes name, address, contact information and medical history.

Preferably, in the step (1), the blood is subjected to anticoagulation treatment after the drawing.

Preferably, in the step (3), the examination items of the high risk group include digital rectal examination, electronic colonoscopy, fecal occult blood test, CEA examination and CT scan examination.

Preferably, in the step (3), the personal information of the other people is not leaked in the process of informing the high risk group.

Preferably, in the step (4), the extracted RNA is stored in an environment at-80 ℃ before gene detection.

Preferably, in the step (4), the personal information corresponding to the sample is repeatedly compared and confirmed in the process of taking out the sample.

Preferably, in the step (5), it is confirmed whether the samples a and B are contaminated before the detection.

Preferably, in the step (6), the archive a and the archive B are classified and stored after being created.

Preferably, in the step (7), the frequency of the retest of the population with the profile A is once in three months or half a year.

Compared with the prior art, the invention has the following beneficial effects:

the invention can provide effective detection and early warning for high-risk people, and enables the high-risk people to pay attention to risks in advance, thereby solving the problems that the rectal cancer patients are directly and later ill high-risk people due to certain genetic probability of the rectal cancer, the current early warning method has the defect of low detection efficiency, the ill risks of the rectal cancer cannot be found as early as possible, the high-risk people cannot be effectively detected and early warned, and the illness state is easily delayed.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

An early colorectal cancer early warning method based on RT-QPCR, which comprises the following steps:

(1) blood drawing: firstly, recording personal information of a crowd with colorectal cancer diseases, and simultaneously extracting blood of the crowd of patients to prepare a sample A and storing the sample A;

(2) screening high risk groups: calling user registration information from a local community database for matching, and recording personal information of direct offspring of people with colorectal cancer diseases;

(3) high risk group detection: informing the high-risk people to go to a hospital for physical examination, if the early symptoms of the rectal cancer exist, immediately treating, and if the early symptoms of the rectal cancer do not exist, extracting blood to prepare a sample B and storing the sample B;

(4) gene extraction: taking a sample A of a patient and a sample B of a direct offspring of the patient, and then extracting and storing RNA of the sample A and the sample B;

(5) gene detection: performing gene testing on RNA extracted from the sample A and the sample B by using an RT-QPCR technology, comparing the susceptible conditions of the samples, wherein if the sample B is easy to infect, the probability of illness of the sample corresponding to a detection person is higher, and if the sample B is not easy to infect or does not infect, the probability of illness of the sample corresponding to the detection person is lower;

(6) and (3) carrying out crowd statistics: the personal information of the crowd with higher prevalence rate is intensively counted and made into a file A, and the personal information of the crowd with lower prevalence rate is intensively counted and made into a file B;

(7) and (4) notification and reinspection: and informing the crowd in the file A of the detection condition, informing the crowd in the file B of the detection condition at regular intervals, informing the crowd in the file B of the detection condition at irregular intervals to go to the hospital for recheck, and recording and storing recheck information of different crowds.

The first embodiment is as follows:

an early colorectal cancer early warning method based on RT-QPCR, which comprises the following steps:

(1) blood drawing: firstly, recording personal information of a crowd with colorectal cancer diseases, simultaneously extracting blood of the crowd of patients to prepare a sample A and storing the sample A, wherein the recorded personal information comprises names, addresses, contact ways and disease history so as to repeatedly confirm the disease of the patients and facilitate staff to contact the direct offspring of the patients;

(2) screening high risk groups: calling user registration information from a local community database for matching, and recording personal information of direct offspring of people with colorectal cancer diseases;

(3) high risk group detection: informing the high risk group to go to a hospital for physical examination, if the early symptoms of rectal cancer exist, immediately treating, if the early symptoms of rectal cancer do not exist, extracting blood to prepare a sample B and storing the sample B, wherein the examination items of the high risk group include digital rectal examination, electronic colonoscopy, fecal occult blood test, CEA examination and CT scanning examination, and repeatedly confirming the illness state of the high risk group through various examinations;

(4) gene extraction: taking a sample A of a patient and a sample B of a direct offspring of the patient, then extracting and storing RNA of the sample A and the sample B, wherein the extracted RNA is stored in an environment at-80 ℃ before gene detection so as to prevent the inactivation of the RNA before the detection and influence on a detection result;

(5) gene detection: carrying out gene testing on RNA extracted from a sample A and a sample B by using RT-QPCR technology, comparing susceptibility conditions of the samples, if the sample B is easy to infect, indicating that the prevalence probability of the sample corresponding to a detection person is higher, and if the sample B is not easy to infect or not infect, indicating that the prevalence probability of the sample corresponding to the detection person is lower, wherein whether the sample A and the sample B are polluted or not is confirmed before detection, and the detection result is easily influenced by the detection of the polluted sample, so that misdiagnosis is caused;

(6) and (3) carrying out crowd statistics: the method comprises the steps of carrying out centralized statistics on personal information of people with high prevalence rate and making the personal information into a file A, carrying out centralized statistics on the personal information of people with low prevalence rate and making the personal information into a file B, wherein the file A and the file B are stored in a classified mode after being made, so that the files are prevented from being mixed up with each other and personal information is prevented from being disordered;

(7) and (4) notification and reinspection: the detection state of the crowd of the file A is informed, the crowd of the file B is informed to go to a hospital regularly for recheck, the detection state of the crowd of the file B is informed, the crowd of the file B is informed to go to the hospital irregularly for recheck, the recheck information of different crowds is recorded and stored, wherein the recheck frequency of the crowd of the file A is once every three months or half a year, and the physical condition of the crowd is confirmed repeatedly by carrying out the periodical recheck on the crowd so as to carry out early warning before the patient suffers from a disease.

Example two:

an early colorectal cancer early warning method based on RT-QPCR, which comprises the following steps:

(1) blood drawing: firstly, recording personal information of people suffering from colorectal cancer diseases, simultaneously extracting blood of patient groups to prepare a sample A and storing the sample A, wherein the recorded personal information comprises names, addresses, contact ways and disease history so as to repeatedly confirm the disease condition of the patient, and simultaneously facilitate workers to contact the direct offspring of the patient, and the blood is subjected to anticoagulation treatment after extraction so as to prevent the blood from coagulating and further influence the subsequent operation;

(2) screening high risk groups: calling user registration information from a local community database for matching, and recording personal information of direct offspring of people with colorectal cancer diseases;

(3) high risk group detection: informing high risk people to go to a hospital for physical examination, if early symptoms of rectal cancer exist, immediately treating, if no symptoms exist, extracting blood to prepare a sample B and storing the sample B, wherein the examination items of the high risk people include digital rectal examination, electronic colonoscopy, fecal occult blood test, CEA examination and CT scanning examination, repeatedly confirming the illness state of the high risk people through various examinations, and simultaneously informing the high risk people that the personal information of other people is not leaked in the process so as to prevent embarrassment of relevant people or discrimination by other people;

(4) gene extraction: taking out a sample A of a patient and a sample B of a direct offspring of the patient, then extracting and storing RNA of the sample A and the sample B, wherein the extracted RNA is stored in an environment at-80 ℃ before gene detection so as to prevent the inactivation of the RNA before the detection and influence on a detection result, and meanwhile, repeatedly comparing and confirming personal information corresponding to the sample in the process of taking out the sample so as to prevent the misdiagnosis of other people caused by the disorder of the sample and the personal information;

(5) gene detection: carrying out gene testing on RNA extracted from a sample A and a sample B by using RT-QPCR technology, comparing susceptibility conditions of the samples, if the sample B is easy to infect, indicating that the prevalence probability of the sample corresponding to a detection person is higher, and if the sample B is not easy to infect or not infect, indicating that the prevalence probability of the sample corresponding to the detection person is lower, wherein whether the sample A and the sample B are polluted or not is confirmed before detection, and the detection result is easily influenced by the detection of the polluted sample, so that misdiagnosis is caused;

(6) and (3) carrying out crowd statistics: the method comprises the steps of carrying out centralized statistics on personal information of people with high prevalence rate and making the personal information into a file A, carrying out centralized statistics on the personal information of people with low prevalence rate and making the personal information into a file B, wherein the file A and the file B are stored in a classified mode after being made, so that the files are prevented from being mixed up with each other and personal information is prevented from being disordered;

(7) and (4) notification and reinspection: the detection state of the crowd of the file A is informed, the crowd of the file B is informed to go to a hospital regularly for recheck, the detection state of the crowd of the file B is informed, the crowd of the file B is informed to go to the hospital irregularly for recheck, the recheck information of different crowds is recorded and stored, wherein the recheck frequency of the crowd of the file A is once every three months or half a year, and the physical condition of the crowd is confirmed repeatedly by carrying out the periodical recheck on the crowd so as to carry out early warning before the patient suffers from a disease.

The invention can provide effective detection and early warning for high-risk people, and enables the high-risk people to pay attention to risks in advance, thereby solving the problems that the rectal cancer patients are directly and later ill high-risk people due to certain genetic probability of the rectal cancer, the current early warning method has the defect of low detection efficiency, the ill risks of the rectal cancer cannot be found as early as possible, the high-risk people cannot be effectively detected and early warned, and the illness state is easily delayed.

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as a starting material. In this method, total or messenger RNA (mrna) is first transcribed into complementary dna (cdna) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as a template. RT-qPCR has been used in a variety of molecular biological applications including gene expression analysis, RNA interference validation, microarray validation, pathogen detection, gene testing, and disease research.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.