阅读说明：本技术 一种用于核酸提取的齿轮箱 (A gear box for nucleic acid extraction ) 是由 邵光业 其他发明人请求不公开姓名 于 2021-02-15 设计创作，主要内容包括：本申请揭示一种用于核酸提取的齿轮箱,包含：主动轮、从动轮、齿轮箱盖、齿轮箱体、滚珠；在齿轮箱盖和从动轮以及齿轮箱体之间分别设置有滚珠,从动轮的侧面设置有磁体固定槽,齿轮箱盖设置有从动轮滚珠槽,齿轮箱体设置有从动轮滚珠槽。通过主动轮带动从动轮转动,各个从动轮分别带动磁体绕核酸提取试管转动,带动试管内的磁珠转动从而实现核酸提取过程。本发明极大的简化了核酸提取扩增检测一体化设备的结构,缩减了一体化设备的体积,降低了一体化设备的成本,可以大幅提高一体化设备的样本检测量。(The present application discloses a gear box for nucleic acid extraction, comprising: the driving wheel, the driven wheel, the gearbox cover, the gearbox body and the balls; the gear box cover is provided with a driven wheel ball groove, and the gear box body is provided with a driven wheel ball groove. The driving wheel drives the driven wheels to rotate, and each driven wheel drives the magnet to rotate around the nucleic acid extraction test tube respectively, so that the magnetic beads in the test tube are driven to rotate, and the nucleic acid extraction process is realized. The invention greatly simplifies the structure of the integrated equipment for extracting, amplifying and detecting nucleic acid, reduces the volume of the integrated equipment, reduces the cost of the integrated equipment and can greatly improve the sample detection amount of the integrated equipment.)

1. A gearbox for nucleic acid extraction, comprising:

the driving wheel is connected with an external driving mechanism;

the driven wheels are of annular structures, ball grooves are formed in the two sides of each driven wheel, and the driven wheels are arranged in an annular or rectangular array;

the gearbox cover is provided with a driven wheel ball groove;

the gearbox body is provided with a driven wheel ball groove;

and the ball is arranged in the ball groove of the driven wheel.

2. The gearbox for nucleic acid extraction according to claim 1, wherein the ball grooves on the driven wheel are hemispherical grooves in an annular array or annular grooves with semicircular sections.

3. The gear box for nucleic acid extraction according to claim 1, wherein the driven wheel is provided with a magnet fixing groove.

4. The gear box for nucleic acid extraction according to claim 1, wherein the driven wheel ball groove of the gear box cover is an annular groove with a semicircular section.

5. The gear box for nucleic acid extraction according to claim 1, wherein the driven wheel ball groove of the gear box body is an annular groove with a semicircular section.

6. The gearbox for nucleic acid extraction according to claim 1, wherein said driven wheels are engaged with each other.

7. The gearbox for nucleic acid extraction according to claim 1, wherein the driven wheel is connected with the gearbox cover through a ball bearing.

8. The gearbox for nucleic acid extraction according to claim 1, wherein the driven wheel is connected with the gearbox housing through a ball bearing.

9. The gearbox for nucleic acid extraction according to claim 1, wherein the driven wheel ball groove of the gearbox cover is provided with a concentric through hole.

10. The gearbox for nucleic acid extraction according to claim 1, wherein the driven wheel ball groove of the gearbox housing is provided with a through hole.

Technical Field

The invention belongs to the field of molecular diagnosis, and relates to a gear box for nucleic acid extraction.

Background

The principle of magnetic bead method for extracting nucleic acid is that according to the same principle as that of silica gel membrane centrifugal column, the surface of superparamagnetic nano-particles is modified and surface-modified by using nano-technology to prepare superparamagnetic silica nano-magnetic beads. The magnetic beads can be specifically identified and efficiently combined with nucleic acid molecules on a microscopic interface. By utilizing the superparamagnetism of the nanometer magnetic microspheres coated by the silicon dioxide, under the action of Chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate and the like) and an external magnetic field, DNA and RNA can be separated from samples such as blood, animal tissues, food, pathogenic microorganisms and the like, and the magnetic microsphere can be applied to multiple fields such as clinical disease diagnosis, blood transfusion safety, forensic medicine identification, environmental microorganism detection, food safety detection, molecular biology research and the like.

The nucleic acid amplification detection technology is a process of providing in vitro amplification conditions for nucleic acid fragments, enabling the nucleic acid fragments to be amplified in an exponential manner in a large quantity, adding a fluorescent dye or a fluorescent marker in the nucleic acid amplification process, detecting the intensity of a fluorescent signal by adopting an optical device, and obtaining a nucleic acid amplification result by processing and analyzing the fluorescent signal.

The steps of cracking, cleaning, eluting, amplifying and the like are included in the process of nucleic acid extraction and amplification detection, the current mainstream technology must be carried out step by step in a standard laboratory, the whole process is easy to pollute the sample nucleic acid, the operation difficulty is high, the requirement on operators is high, and the popularization of the nucleic acid detection technology is not facilitated. In order to solve the defects of the prior art, the integrated technology of nucleic acid extraction, amplification and detection needs to be developed. The nucleic acid extraction, amplification and detection integrated technology comprises multiple steps of nucleic acid cracking, cleaning, elution, amplification and the like, so that the existing integrated equipment is generally complex in design, large in size, high in cost and small in detection sample amount, and the factors restrict the popularization of the nucleic acid extraction, amplification and detection integrated technology.

Disclosure of Invention

In order to solve the disadvantages of the prior art, the present invention provides a gear box for nucleic acid extraction, comprising:

the driving wheel is connected with an external driving mechanism;

the driven wheels are of annular structures, ball grooves are formed in the two sides of each driven wheel, and the driven wheels are arranged in an annular or rectangular array;

the gearbox cover is provided with a driven wheel ball groove;

the gearbox body is provided with a driven wheel ball groove;

and the ball is arranged in the ball groove of the driven wheel.

Preferably, the ball grooves on the driven wheel are hemispherical grooves in an annular array or annular grooves with semicircular sections.

Preferably, a magnet fixing groove is formed on the driven wheel.

Preferably, the driven wheel ball groove of the gear box cover is an annular groove with a semicircular section.

Preferably, the driven wheel ball groove of the gear box body is an annular groove with a semicircular section.

Preferably, the driven wheels are mutually meshed.

Preferably, the driven wheel is connected with the gearbox cover through a ball.

Preferably, the driven wheel is connected with the gear box body through a ball.

Preferably, a concentric through hole is formed in a driven wheel ball groove of the gear box cover.

Preferably, a through hole is formed in a driven wheel ball groove of the gear box body.

Compared with the prior art, the gain effect of the invention is as follows: the invention greatly simplifies the structure of the integrated equipment for extracting, amplifying and detecting nucleic acid, reduces the volume of the integrated equipment, reduces the cost of the integrated equipment and can greatly improve the sample detection amount of the integrated equipment.

Drawings

FIG. 1 is a perspective view of a gear box structure for nucleic acid extraction.

FIG. 2 is a side sectional view of a gear box for nucleic acid extraction.

Fig. 3 is a perspective view of the driven wheel structure.

Fig. 4 is a perspective view of a gearbox cover structure.

Fig. 5 is a perspective view of the gear housing structure.

Detailed Description

The technical embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings of the present invention.

As shown in FIGS. 1 and 2, the present invention relates to a gear box for nucleic acid extraction, comprising: the gear box comprises a driving wheel (1), a middle driven wheel (202), a left driven wheel (203), a right driven wheel (201), a gear box cover (3), a gear box body (4), balls (5), an upper bearing (6) and a lower bearing (7).

As shown in fig. 1 and 2, a driving wheel (1) is meshed with a middle driven wheel (202), the middle driven wheel (202) is meshed with a left driven wheel (203), and a right driven wheel (201) is respectively meshed; balls (5) are respectively arranged between the gear box cover (3) and the middle driven wheel (202), the left driven wheel (203), the right driven wheel (201) and the gear box body (4), and an upper bearing (6) and a lower bearing (7) are arranged on two sides of the driving wheel (1). As shown in fig. 3, a magnet fixing groove is provided at a side surface of the driven wheel. The gearbox cover (3) is provided with a driven wheel ball groove as shown in figure 4. The gear housing (4) is provided with a driven wheel ball groove as shown in fig. 5.

In the nucleic acid extraction process, the nucleic acid extraction test tube sets up downthehole from the driving wheel, and the external motor passes through in driving wheel (1) drives from driving wheel (202) and rotates, and well from driving wheel (202) drive a left side and rotate from driving wheel (203) and right side (201), and each drives the magnet respectively from the driving wheel and rotates around the nucleic acid extraction test tube, thereby it rotates to drive the magnetic bead in the test tube and realizes the nucleic acid extraction process.

It is to be understood that: although the present invention has been described in detail, the present invention is only for the purpose of illustration and not limitation, and any invention that does not depart from the spirit of the present invention falls within the scope of the present invention.