阅读说明：本技术 Egfr的亲和力成熟结合蛋白及应用 (Affinity maturation binding protein of EGFR (epidermal growth factor receptor) and application ) 是由 魏星 陈涛 于 2020-12-25 设计创作，主要内容包括：本发明公开了一种抗人EGFR的亲和力成熟结合蛋白及应用。该亲和力成熟结合蛋白是氨基酸序列如SEQ ID NO:4所示的aEG22C4亲和力成熟结合蛋白；或是氨基酸序列如SEQ ID NO:2所示的aEG12E2亲和力成熟结合蛋白、氨基酸序列如SEQ ID NO:3所示的aEG13E8亲和力成熟结合蛋白和氨基酸序列如SEQ ID NO:1所示的aEG11A6亲和力成熟结合蛋白中的至少一种与aEG22C4亲和力成熟结合蛋白组合形成的结合蛋白。该亲和力成熟结合蛋白具有高亲和力、高特异性和低免疫原性,并且具有较好的稳定性,结构简单,易于进行工程化改造等优点,可更好地应用于结合蛋白药物的开发。(The invention discloses an affinity maturation binding protein for resisting human EGFR and application thereof. The affinity maturation binding protein is aEG22C4 affinity maturation binding protein with the amino acid sequence shown as SEQ ID NO. 4; or a binding protein formed by combining at least one of aEG12E2 affinity maturation binding protein with an amino acid sequence shown as SEQ ID NO. 2, aEG13E8 affinity maturation binding protein with an amino acid sequence shown as SEQ ID NO. 3 and aEG11A6 affinity maturation binding protein with an amino acid sequence shown as SEQ ID NO. 1 with aEG22C4 affinity maturation binding protein. The affinity mature binding protein has the advantages of high affinity, high specificity, low immunogenicity, good stability, simple structure, easy engineering modification and the like, and can be better applied to the development of binding protein drugs.)