阅读说明：本技术 一种高效提取蝉花虫草子实体中n6-(2-羟乙基)腺苷的方法 (Method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera sporocarp ) 是由 付婷伟 邵颖 陈安徽 彭双双 吕韩 刘妍妍 何晓波 姚泽润 于 2021-05-20 设计创作，主要内容包括：本发明属于食用菌活性成分提取技术领域,具体涉及一种高效提取蝉花虫草子实体中N6-(2-羟乙基)腺苷的方法。本发明所述高效提取蝉花虫草子实体中N6-(2-羟乙基)腺苷的方法,在现有技术传统的乙醇提取-树脂分离-制备型色谱分离的提取方法的基础上,通过在乙醇提取步骤中加入肌氨酸的方式进行辅助提取,有助于提高N6-(2-羟乙基)腺苷的提取效率。(The invention belongs to the technical field of extraction of active ingredients of edible fungi, and particularly relates to a method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera fruiting bodies. The method for efficiently extracting the N6- (2-hydroxyethyl) adenosine from the cordyceps sobolifera sporocarp is used for carrying out auxiliary extraction by adding sarcosine in the ethanol extraction step on the basis of the traditional ethanol extraction-resin separation-preparative chromatographic separation extraction method in the prior art, and is beneficial to improving the extraction efficiency of the N6- (2-hydroxyethyl) adenosine.)

1. A method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera sporocarp is characterized by comprising the following steps:

(1) pulverizing Cordyceps cicadae Miquel fruiting body, adding ethanol solution, mixing, and adding sarcosine for auxiliary extraction;

(2) collecting the extractive solution, separating with resin, eluting with low concentration ethanol solution, collecting eluate, concentrating, and drying;

(3) dissolving the concentrate with low concentration methanol solution, separating by reverse phase high performance liquid chromatography, collecting 14.6-15.2min chromatogram peak component in the chromatogram, and drying.

2. The method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps cicadae miq fruiting bodies according to claim 1, wherein in the step (1), the addition amount of the sarcosine is 3-8 wt% of the cordyceps cicadae miq fruiting bodies.

3. The method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera fruiting bodies according to claim 1 or 2, wherein in the step (1), the concentration of the ethanol solution is 30-50 v/v%.

4. The method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera fruiting bodies according to any one of claims 1 to 3, wherein in the step (1), the dosage of the ethanol solution is 2-4 times of that of the cordyceps sobolifera fruiting bodies.

5. The method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera fruiting bodies according to any one of claims 1 to 4, wherein in the step (2), the resin is D101 macroporous resin.

6. The method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera fruiting bodies according to any one of claims 1 to 5, wherein in the step (2), the dosage ratio of the fruiting bodies to the resin is controlled to be 1: 8-12 g/mL.

7. The method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera fruiting bodies according to any one of claims 1 to 6, wherein in the step (2), the eluent is ethanol solution with volume concentration of 15-25 v/v%, and the use amount is 3-5 times of column volume.

8. The method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera fruiting bodies according to any one of claims 1 to 7, wherein in the step (3), the low-concentration methanol is a methanol solution with a volume fraction of 10-20 v/v%, and a sample concentration is configured to be 50-150 mg/ml.

9. The method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera fruiting bodies according to any one of claims 1 to 8, wherein in the step (3), the reverse phase high performance liquid preparative chromatography conditions comprise:

a chromatographic column: c18 bonded phase packing column;

mobile phase: 10-20 v/v% methanol-water solution;

detection wavelength: an ultraviolet detector is 260 nm;

flow rate of mobile phase: 160 ml/min;

sample introduction amount: 10 ml.

10. The method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera fruiting body according to any one of claims 1 to 9, wherein the cordyceps sobolifera fruiting body is a fruiting body with high content of N6- (2-hydroxyethyl) adenosine.

Technical Field

The invention belongs to the technical field of extraction of active ingredients of edible fungi, and particularly relates to a method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera fruiting bodies.

Background

N6- (2-hydroxyethyl) adenosine (N6- (2-hydroxyethyl) adenosine, HEA) is adenosine derivative extracted from Cordyceps cicadae mycelium, is a specific component of Cordyceps, and has reported functions including antibacterial, anticancer and anticonvulsant effects, and has Ca²⁺Antagonism and muscle contraction activity. HEA is the first biological source of calcium ion antagonist known at present, and is also an adenosine A1 receptor agonist, and the use of DPCPX (adenosine A1 receptor antagonist) can prove that HEA acts on adenosine A1 receptor, while the unique medicinal value of Cordyceps has promoted the development of pharmacological research. At present, the content of HEA is an important index for measuring the quality of cordyceps sinensis, but the content of HEA is very low no matter natural cordyceps sinensis or artificial cordyceps sinensis, so that certain difficulty is caused for extracting HEA, and the problem of low extraction efficiency generally exists.

Disclosure of Invention

Therefore, the technical problem to be solved by the invention is to provide a method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera fruiting bodies so as to solve the problem of low HEA extraction rate in the prior art.

In order to solve the technical problems, the method for efficiently extracting the N6- (2-hydroxyethyl) adenosine from the cordyceps sobolifera sporocarp comprises the following steps:

(1) pulverizing Cordyceps cicadae Miquel fruiting body, adding ethanol solution, mixing, and adding sarcosine for auxiliary extraction;

(2) collecting the extractive solution, separating with resin, eluting with low concentration ethanol solution, collecting eluate, concentrating, and drying;

(3) dissolving the concentrate with low concentration methanol solution, separating by reverse phase high performance liquid chromatography, collecting 14.6-15.2min chromatogram peak component in the chromatogram, and drying.

Specifically, in the step (1), the addition amount of the sarcosine accounts for 3-8 wt% of the using amount of the cordyceps sobolifera sporocarp.

Specifically, in the step (1), the concentration of the ethanol solution is 30-50 v/v%.

Specifically, in the step (1), the dosage of the ethanol solution is 2-4 times of the dosage of the cordyceps sobolifera sporocarp.

Specifically, in the step (2), the resin is D101 macroporous resin.

Specifically, in the step (2), the ratio of the used amount of the fruit body to the used amount of the resin is controlled to be 1: 8-12 g/mL.

Specifically, in the step (2), the eluent is ethanol solution with volume concentration of 15-25 v/v%, and the using amount of the eluent is 3-5 times of the column volume.

Specifically, in the step (3), the low-concentration methanol is a methanol solution with a volume fraction of 10-20 v/v%, and the prepared sample concentration is 50-150 mg/ml.

Specifically, in the step (3), the reverse-phase high performance liquid preparative chromatography conditions include:

a chromatographic column: c18 bonded phase packing column;

mobile phase: 10-20 v/v% methanol-water solution;

detection wavelength: an ultraviolet detector is 260 nm;

flow rate of mobile phase: 160 ml/min;

sample introduction amount: 10 ml.

Specifically, the cordyceps sobolifera fruiting body is a fruiting body with high content of N6- (2-hydroxyethyl) adenosine.

The cordyceps cicadae miq fruiting body with the high content of N6- (2-hydroxyethyl) adenosine is obtained by cultivating the cordyceps cicadae miq fruiting body with a method capable of improving the content of N6- (2-hydroxyethyl) adenosine in the cordyceps cicadae miq fruiting body, and specifically comprises the steps of carrying out liquid strain culture in a liquid culture medium and carrying out fruiting body culture in a solid culture medium;

the solid culture medium comprises the following components in a mass ratio of 1: 0.8-1.5 of solid matrix and nutrient solution;

the solid matrix comprises the following components in parts by weight: 20-30 parts of corn grit, 10-20 parts of bran, 10-20 parts of cicada pupa powder, 3-8 parts of mulberry twig particles, 5-12 parts of tobacco leaf and stem particles and 0.5-2 parts of EM (effective microorganism) bacteria powder;

the nutrient solution comprises the following components in percentage by mass: 20-30g/L of carbon source, 20-30g/L of nitrogen source, 5-12g/L of inorganic salt and 6-8 of pH.

Specifically, the cultivation method for improving the content of N6- (2-hydroxyethyl) adenosine in cordyceps sobolifera fruiting bodies comprises the following steps:

the carbon source comprises glucose;

the nitrogen source comprises peptone;

the inorganic salt comprises a mixture of potassium dihydrogen phosphate, dipotassium hydrogen phosphate and magnesium sulfate.

Specifically, the sporocarp culturing step comprises a spawn running step, a color conversion step and a sporocarp management step.

Specifically, the control conditions of the spawn running step comprise: culturing at 15-18 deg.C and humidity of 60-80% under dark condition.

Specifically, the control conditions of the color conversion step include: controlling the illumination condition to be 200-500lux, and controlling the photoperiod light-dark ratio to be L15-18: d6-9, the culture temperature is 15-18 ℃, and the humidity is 60-80%.

Specifically, the control conditions of the step of managing a fruit body include: controlling the illumination condition to be 200-500lux, and controlling the photoperiod light-dark ratio to be L15-18: d6-9, the culture temperature is 20-25 ℃, and the humidity is 60-80%.

Specifically, the liquid culture medium comprises the following components in percentage by mass: 2-6% of glucose, 1-2% of egg white, 1-3% of peptone and MgSO₄·7H₂O 0.05％、KH₂PO₄0.05% and natural pH.

Specifically, the conditions for culturing the liquid strain comprise: the fermentation temperature is controlled to be 20-25 ℃, and the stirring speed is 150 and 180 rpm.

Specifically, the method further comprises the step of carrying out conventional activation on the strain before the step of culturing the liquid strain.

The invention also discloses cordyceps sobolifera sporocarp obtained by the cultivation method.

The method for efficiently extracting the N6- (2-hydroxyethyl) adenosine from the cordyceps sobolifera sporocarp is used for carrying out auxiliary extraction by adding sarcosine in the ethanol extraction step on the basis of the traditional ethanol extraction-resin separation-preparative chromatographic separation extraction method in the prior art, and is beneficial to improving the extraction efficiency of the N6- (2-hydroxyethyl) adenosine.

According to the cultivation method for improving the content of N6- (2-hydroxyethyl) adenosine in the cordyceps sobolifera fruiting body, on the basis of the conventional cordyceps sobolifera fruiting body cultivation process, the accumulation of N6- (2-hydroxyethyl) adenosine in the fermentation process is effectively induced by screening the solid culture medium substrate, and the content of N6- (2-hydroxyethyl) adenosine in the cordyceps sobolifera fruiting body is improved. The method of the invention is specifically directed to the accumulation of N6- (2-hydroxyethyl) adenosine during fermentation, and is particularly significant in the increase of the content of N6- (2-hydroxyethyl) adenosine compared with the general scheme optimized for adenosine.

Detailed Description

In the following preparation examples of the present invention, the selected cordyceps sobolifera-cultivating strains are conventionally known strains in the prior art, and the cordyceps sobolifera strains selected in the following examples are purchased from Shanghai Haisheng industries, Ltd.

In the following preparation examples of the present invention, the cordyceps sobolifera is subjected to liquid culture to obtain corresponding culture solutions (seed solutions), and then inoculated into corresponding solid culture media to perform fermentation of fruit bodies. In the following preparation examples, the liquid medium used in the liquid culture step includes the following components by mass: glucose 4%, egg white 1%, peptone 2%, MgSO₄·7H₂O 0.05％、KH₂PO₄0.05% and natural pH. After sterilization, the preserved strains are dug and inoculated into the liquid culture medium under the aseptic condition, the fermentation temperature is controlled to be 22 ℃, the stirring speed is 160rpm, and the shake flask culture is carried out for 48 hours, so as to obtain the seed liquid to be inoculated for solid fermentation of the sporocarp in the following embodiment for standby.

Preparation example 1

In this preparation example, the seed liquid obtained as described above was cultured in a solid medium to obtain a fruit body.

The solid matrix was formulated at the following levels: 20 parts of corn grit, 20 parts of bran, 10 parts of cicada pupa powder, 8 parts of mulberry twig particles, 5 parts of tobacco leaf and stem particles and 2 parts of EM (effective microorganism) powder (temporarily not added);

the nutrient solution is prepared according to the following contents: 20g/L glucose, 30g/L peptone, 2g/L potassium dihydrogen phosphate, 1g/L dipotassium hydrogen phosphate and 2g/L magnesium sulfate;

according to the following steps of 1: 0.8, mixing the solid culture medium (except EM bacteria powder) and the nutrient solution uniformly, sealing the prepared culture medium, placing the culture medium in a high-pressure moist heat sterilization pot, and performing conventional sterilization at 121 ℃ for 40 min.

Inoculating the seed solution into the solid culture medium according to the inoculation amount of 10 wt% under the room temperature and aseptic condition, adding the EM bacterial powder with corresponding amount, uniformly mixing, then placing the inoculated culture medium into a cultivation room for cultivation, controlling the temperature at 17 ℃ and the humidity at 70%, and performing light-tight cultivation for 3-5 days until the spawn running is complete.

And then adjusting the illumination condition, controlling the illumination condition to be 300lux, and controlling the photoperiod light-dark ratio to be L16: d8, continuously controlling the culture temperature to be 17 ℃ and the humidity to be 70%, continuously culturing for 5-8 days to finish color conversion and form the stroma bud.

And then maintaining the illumination condition, continuously controlling the illumination condition to be 300lux, and controlling the photoperiod light-dark ratio to be L16: d8, controlling the culture temperature at 22 deg.C and humidity at 70%, continuously culturing for 15-20 days, collecting mature fruiting body, and oven drying.

Preparation example 2

In this preparation example, the seed liquid obtained as described above was cultured in a solid medium to obtain a fruit body.

The solid matrix was formulated at the following levels: 30 parts of corn grit, 10 parts of bran, 20 parts of cicada pupa powder, 3 parts of mulberry twig particles, 12 parts of tobacco leaf and stem particles and 0.5 part of EM (effective microorganism) powder (temporarily not added);

the nutrient solution is prepared according to the following contents: 30g/L glucose, 20g/L peptone, 4g/L potassium dihydrogen phosphate, 4g/L dipotassium hydrogen phosphate and 4g/L magnesium sulfate;

according to the following steps of 1: 1.5, uniformly mixing the solid culture medium (except EM bacteria powder) and the nutrient solution, sealing the prepared culture medium, placing the culture medium in a high-pressure moist heat sterilization pot, and conventionally sterilizing for 40min at 121 ℃.

Inoculating the seed solution into the solid culture medium according to the inoculation amount of 10 wt% under the room temperature and aseptic condition, adding the EM bacterial powder with corresponding amount, uniformly mixing, then placing the inoculated culture medium into a cultivation room for cultivation, controlling the temperature at 17 ℃ and the humidity at 70%, and performing light-tight cultivation for 3-5 days until the spawn running is complete.

And then adjusting the illumination condition, controlling the illumination condition to be 300lux, and controlling the photoperiod light-dark ratio to be L16: d8, continuously controlling the culture temperature to be 17 ℃ and the humidity to be 70%, continuously culturing for 5-8 days to finish color conversion and form the stroma bud.

And then maintaining the illumination condition, continuously controlling the illumination condition to be 300lux, and controlling the photoperiod light-dark ratio to be L16: d8, controlling the culture temperature at 22 deg.C and humidity at 70%, continuously culturing for 15-20 days, collecting mature fruiting body, and oven drying.

Preparation example 3

In this preparation example, the seed liquid obtained as described above was cultured in a solid medium to obtain a fruit body.

The solid matrix was formulated at the following levels: 25 parts of corn grit, 15 parts of bran, 15 parts of cicada pupa powder, 5 parts of mulberry twig particles, 8 parts of tobacco leaf and stem particles and 1 part of EM (effective microorganism) powder (temporarily not added);

the nutrient solution is prepared according to the following contents: 25g/L glucose, 25g/L peptone, 3g/L potassium dihydrogen phosphate, 2g/L dipotassium hydrogen phosphate and 3g/L magnesium sulfate;

according to the following steps of 1: 1.2, uniformly mixing the solid culture medium (except EM bacterial powder) and the nutrient solution according to the mass ratio, sealing the prepared culture medium, placing the culture medium in a high-pressure moist heat sterilization pot, and conventionally sterilizing for 40min at 121 ℃.

Inoculating the seed solution into the solid culture medium according to the inoculation amount of 10 wt% under the room temperature and aseptic condition, adding the EM bacterial powder with corresponding amount, uniformly mixing, then placing the inoculated culture medium into a cultivation room for cultivation, controlling the temperature at 17 ℃ and the humidity at 70%, and performing light-tight cultivation for 3-5 days until the spawn running is complete.

And then adjusting the illumination condition, controlling the illumination condition to be 300lux, and controlling the photoperiod light-dark ratio to be L16: d8, continuously controlling the culture temperature to be 17 ℃ and the humidity to be 70%, continuously culturing for 5-8 days to finish color conversion and form the stroma bud.

And then maintaining the illumination condition, continuously controlling the illumination condition to be 300lux, and controlling the photoperiod light-dark ratio to be L16: d8, controlling the culture temperature at 22 deg.C and humidity at 70%, continuously culturing for 15-20 days, collecting mature fruiting body, and oven drying.

Preparation example 4

The method for culturing cordyceps sobolifera sporocarp in the preparation example is the same as that in the preparation example 3, and only the EM bacterial powder is not contained in the solid matrix.

Preparation example 5

The method for culturing cordyceps sobolifera sporocarp in the preparation example is the same as that in the preparation example 3, and the difference is only that the tobacco leaf and stem particles are not contained in the solid matrix.

Preparation example 6

The method for culturing cordyceps sobolifera sporocarp in the preparation example is the same as that in the preparation example 3, and only the mulberry twig crushed grains are not contained in the solid matrix.

Example 1

Crushing 100g of cordyceps sobolifera fruiting body harvested in the preparation example 3, adding 3 times of 40 v/v% ethanol solution, uniformly mixing, adding 5g of sarcosine, performing auxiliary extraction, and extracting at normal temperature for 2 hours; collecting extract, filtering with warp cloth, collecting filtrate, separating with D101 macroporous adsorbent resin, and controlling the dosage of resin as fruiting body: resin 1: 10g/ml, eluting with 3 times of column volume of 20 v/v% ethanol solution, collecting eluate, and concentrating; dissolving the concentrate with 15% methanol aqueous solution by volume fraction, preparing into 100mg/ml test solution, filtering with 0.45 μm microporous membrane, and separating by preparative High Performance Liquid Chromatography (HPLC) under the conditions of:

a chromatographic column: c18 bonded phase filler;

sample introduction volume: 10 ml;

eluent: 15% strength by volume methanol-water solution

Flow rate of mobile phase: 160 ml/min;

detection wavelength: detecting by an ultraviolet detector at 260 nm;

collecting chromatographic peak components with retention time of 14.6-15.2min, which are fractions of the target product (N6- (2-hydroxyethyl) adenosine), and the appearance is white powder, which is correct via comparison product.

Example 2

Crushing 100g of cordyceps sobolifera fruiting body harvested in the preparation example 4, adding 3 times of 40 v/v% ethanol solution, uniformly mixing, adding 5g of sarcosine, performing auxiliary extraction, and extracting at normal temperature for 2 hours; collecting extract, filtering with warp cloth, collecting filtrate, separating with D101 macroporous adsorbent resin, and controlling the dosage of resin as fruiting body: resin 1: 10g/ml, eluting with 3 times of column volume of 20 v/v% ethanol solution, collecting eluate, and concentrating; dissolving the concentrate with 15% methanol aqueous solution by volume fraction, preparing into 100mg/ml test solution, filtering with 0.45 μm microporous membrane, and separating by preparative High Performance Liquid Chromatography (HPLC) under the conditions of:

a chromatographic column: c18 bonded phase filler;

sample introduction volume: 10 ml;

eluent: 15% strength by volume methanol-water solution

Flow rate of mobile phase: 160 ml/min;

detection wavelength: detecting by an ultraviolet detector at 260 nm;

collecting chromatographic peak components with retention time of 14.6-15.2min, which are fractions of the target product (N6- (2-hydroxyethyl) adenosine), and the appearance is white powder, which is correct via comparison product.

Example 3

Crushing 100g of cordyceps sobolifera fruiting bodies harvested in the preparation example 5, adding 3 times of 40 v/v% ethanol solution, uniformly mixing, adding 5g of sarcosine, performing auxiliary extraction, and extracting at normal temperature for 2 hours; collecting extract, filtering with warp cloth, collecting filtrate, separating with D101 macroporous adsorbent resin, and controlling the dosage of resin as fruiting body: resin 1: 10g/ml, eluting with 3 times of column volume of 20 v/v% ethanol solution, collecting eluate, and concentrating; dissolving the concentrate with 15% methanol aqueous solution by volume fraction, preparing into 100mg/ml test solution, filtering with 0.45 μm microporous membrane, and separating by preparative High Performance Liquid Chromatography (HPLC) under the conditions of:

a chromatographic column: c18 bonded phase filler;

sample introduction volume: 10 ml;

eluent: 15% strength by volume methanol-water solution

Flow rate of mobile phase: 160 ml/min;

detection wavelength: detecting by an ultraviolet detector at 260 nm;

collecting chromatographic peak components with retention time of 14.6-15.2min, which are fractions of the target product (N6- (2-hydroxyethyl) adenosine), and the appearance is white powder, which is correct via comparison product.

Example 4

Crushing 100g of cordyceps sobolifera fruiting bodies harvested in the preparation example 6, adding 3 times of 40 v/v% ethanol solution, uniformly mixing, adding 5g of sarcosine, performing auxiliary extraction, and extracting at normal temperature for 2 hours; collecting extract, filtering with warp cloth, collecting filtrate, separating with D101 macroporous adsorbent resin, and controlling the dosage of resin as fruiting body: resin 1: 10g/ml, eluting with 3 times of column volume of 20 v/v% ethanol solution, collecting eluate, and concentrating; dissolving the concentrate with 15% methanol aqueous solution by volume fraction, preparing into 100mg/ml test solution, filtering with 0.45 μm microporous membrane, and separating by preparative High Performance Liquid Chromatography (HPLC) under the conditions of:

a chromatographic column: c18 bonded phase filler;

sample introduction volume: 10 ml;

eluent: 15% strength by volume methanol-water solution

Flow rate of mobile phase: 160 ml/min;

detection wavelength: detecting by an ultraviolet detector at 260 nm;

collecting chromatographic peak components with retention time of 14.6-15.2min, which are fractions of the target product (N6- (2-hydroxyethyl) adenosine), and the appearance is white powder, which is correct via comparison product.

Comparative example 1

The HEA extraction process of this comparative example differs from example 1 only in that no sarcosine was added in the extraction step.

Examples of the experiments

The N6- (2-hydroxyethyl) adenosine isolated in examples 1-4 and comparative example 1 above was collected, respectively, weighed and the yield thereof was calculated, and reported in Table 1 below.

TABLE 1 extraction of N6- (2-hydroxyethyl) adenosine

	Weight/g	Yield/%
			Example 1	8.15	8.15
Example 2	7.73	7.73
			Example 3	6.43	6.43
Example 4	6.91	6.91
			Comparative example 1	7.41	7.41

Therefore, the extraction method can effectively improve the extraction efficiency of the N6- (2-hydroxyethyl) adenosine in the cordyceps sobolifera.

It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.