Conductive ink for vitamin detection
阅读说明：本技术 一种用于维生素检测的导电油墨 (Conductive ink for vitamin detection ) 是由 陈俊豪 余嘉琪 何流 唐涛 于 2020-12-22 设计创作，主要内容包括：本发明属于油墨印刷技术领域,具体涉及一种用于维生素检测的导电油墨,所述导电油墨按照重量百分比包括：树脂10％-30％、导电浆液12％-28％、溶剂50％-70％、增稠剂0.5％-1％和消泡剂1％-5％；所述导电浆液中含有导电颗粒,且导电颗粒的重量占导电浆液总重量的92％-98％；所述导电油墨的制备方法包括：原料预处理、制备导电浆液和混匀成品。克服了现有技术的不足,对导电油墨的配方进行改进,制备出对某一种及多种维生素有电化学响应的导电油墨,并结合丝网印刷技术,印刷出试纸电极。(The invention belongs to the technical field of printing ink printing, and particularly relates to conductive printing ink for vitamin detection, which comprises the following components in percentage by weight: 10-30% of resin, 12-28% of conductive slurry, 50-70% of solvent, 0.5-1% of thickening agent and 1-5% of defoaming agent; the conductive slurry contains conductive particles, and the weight of the conductive particles accounts for 92% -98% of the total weight of the conductive slurry; the preparation method of the conductive ink comprises the following steps: pretreating raw materials, preparing conductive slurry and uniformly mixing to obtain a finished product. The method overcomes the defects of the prior art, improves the formula of the conductive ink, prepares the conductive ink which has electrochemical response to one or more vitamins, and prints the test paper electrode by combining with the silk screen printing technology.)
1. A conductive ink for vitamin detection, characterized by: the conductive ink comprises the following components in percentage by weight: 10-30% of resin, 12-28% of conductive slurry, 50-70% of solvent, 0.5-1% of thickening agent and 1-5% of defoaming agent;
the conductive slurry contains conductive particles, and the weight of the conductive particles accounts for 92% -98% of the total weight of the conductive slurry.
2. The conductive ink for vitamin detection according to claim 1, wherein: the resin is selected from one or more of methyl phenyl silicone resin, epoxy resin and carboxymethyl cellulose.
3. The conductive ink for vitamin detection according to claim 1, wherein: the conductive particles are selected from one or more of graphite, carbon powder, graphene, carbon nano tubes, fullerene, functionalized graphene, functionalized carbon nano tubes, gold powder, bismuth nitrate and platinum powder.
4. The conductive ink for vitamin detection according to claim 1, wherein: the solvent is selected from one or more of polybutadiene glycol, ethylene glycol monoethyl ether, ethanol, isopropanol, dimethylformamide, propylene glycol, purified water, polyvinylpyrrolidone, DMF and cyclohexanone.
5. The conductive ink for vitamin detection according to claim 1, wherein: the defoaming agent is selected from one of tween-20, triton-100 or sodium dodecyl sulfate.
6. The conductive ink for vitamin detection according to claim 1, wherein: the thickening agent is at least one of glycerol or oleic acid, terpineol and methyl cellulose.
7. A method of preparing the conductive ink for vitamin detection as claimed in any one of claims 1 to 6, wherein: the method comprises the following steps:
(1) weighing the raw materials according to the formula percentage, grinding the conductive particles, and sieving the ground conductive particles with a 400-mesh sieve for later use;
(2) placing the sieved conductive particles in a muffle furnace at the temperature of 400-700 ℃, sintering for 30-100min, taking out and naturally cooling to obtain sintered particles;
(3) adding the sintered particles into an ethanol solution, uniformly mixing by ultrasonic waves, putting the mixed suspension into a rotary evaporator, and evaporating the ethanol solution to obtain viscous slurry to obtain conductive slurry;
(4) and adding resin, a solvent, a defoaming agent and a thickening agent into the conductive liquid, and uniformly mixing to obtain the conductive ink.
8. Use of a conductive ink according to any one of claims 1 to 6 for the detection of vitamins, characterized in that: printing of test paper electrodes for the detection of one or more vitamins.
Technical Field
The invention belongs to the technical field of printing ink printing, and particularly relates to conductive printing ink for vitamin detection.
Background
Vitamins have an important role in the physiological activities of the human body. Deficiencies and excesses of vitamins in the body can cause problems. E.g., vitamin a deficiency, may be associated with a serious defect in adaptive immunity; and mortality associated with measles, diarrhea and other diseases may be reduced by vitamin a supplementation; vitamin B1 deficiency, weakness, general debilitation and gastrointestinal symptoms; the deficiency of vitamin B9 may cause megaloblastic anemia, peripheral neuropathy, myelopathy, metabolic disorders, etc.
The current vitamin detection means are as follows: the method comprises the methods of high performance liquid chromatography, tandem mass spectrometry, chemiluminescence, electrochemical methods and the like, wherein the electrochemical methods have the characteristics of sensitive detection, high response speed and no need of complex sample pretreatment process.
The principle of the electrochemical method for detecting the vitamins is as follows: vitamins are a class of substances that undergo redox reactions. Under certain conditions, the vitamin undergoes redox reaction on the surface of the electrode, and electrons transferred during the redox reaction are detected by the electrode, so that a corresponding response current is generated on the electrode. According to the Faraday's law of electrolysis, the greater the amount of a substance that chemically changes on the surface of an electrode, the greater the amount of electricity that the electrode is charged with. In the electrochemical detection of vitamins, it appears that the more vitamins that undergo redox reactions at the electrode surface, the greater the response current at the electrode. Thus establishing a relationship between the concentration of the vitamin in the sample and the response current at the electrode.
The existing vitamin electrochemical detection technology is based on the traditional glassy carbon electrode to realize the detection of the vitamin. Since glassy carbon electrodes are not disposable electrodes, the electrode surface must be refreshed prior to each use. The existing method for updating the electrode surface is a mechanical grinding method, i.e. according to the fineness gradient of alumina particles, the electrode surface is ground to a mirror surface from coarse to fine. This process is time consuming and labor intensive. And the quality of electrode grinding is directly related to the accuracy of the detection result, so the method has higher requirements on operators.
A screen-printed electrode is an electrode that can be produced industrially on a large scale. The printed electrode has a high degree of uniformity in quality. Because the influence of operators is eliminated, the electrode produced by the method is very suitable for detecting substances with higher detection requirements. Blood glucose test strips are one example of the use of this method.
Although screen-printed electrodes have such benefits, there have been no reports or examples to date of the use of screen-printed electrodes to achieve detection of vitamin content in a sample due to some technical limitations.
Disclosure of Invention
The invention aims to provide conductive ink for vitamin detection, overcomes the defects of the prior art, improves the formula of the conductive ink, prepares the conductive ink with electrochemical response to one or more vitamins, and prints a test paper electrode by combining a silk-screen printing technology.
In order to solve the problems, the technical scheme adopted by the invention is as follows:
a conductive ink for vitamin detection, the conductive ink comprising, in weight percent: 10-30% of resin, 12-28% of conductive slurry, 50-70% of solvent, 0.5-1% of thickening agent and 1-5% of defoaming agent;
the conductive slurry contains conductive particles, and the weight of the conductive particles accounts for 92% -98% of the total weight of the conductive slurry.
Further, the resin is selected from one or more of methyl phenyl silicone resin, epoxy resin and carboxymethyl cellulose.
Further, the conductive particles are selected from one or more of graphite, carbon powder, graphene, carbon nano tubes, fullerene, functionalized graphene, functionalized carbon nano tubes, gold powder, bismuth nitrate and platinum powder.
Further, the solvent is selected from one or more of polybutadiene glycol, ethylene glycol monoethyl ether, ethanol, isopropanol, dimethylformamide, propylene glycol, purified water, polyvinylpyrrolidone, DMF and cyclohexanone.
Further, the defoaming agent is selected from one of tween-20, triton-100 or sodium dodecyl sulfate.
Further, the thickening agent is at least one of glycerol or oleic acid, terpineol and methyl cellulose.
The invention also provides a preparation method of the conductive ink for vitamin detection, which comprises the following steps:
(1) weighing the raw materials according to the formula percentage, grinding the conductive particles, and sieving the ground conductive particles with a 400-mesh sieve for later use;
(2) placing the sieved conductive particles in a muffle furnace at the temperature of 400-700 ℃, sintering for 30-100min, taking out and naturally cooling to obtain sintered particles;
(3) adding the sintered particles into an ethanol solution, uniformly mixing by ultrasonic waves, putting the mixed suspension into a rotary evaporator, and evaporating the ethanol solution to obtain viscous slurry to obtain conductive slurry;
(4) and adding resin, a solvent, a defoaming agent and a thickening agent into the conductive liquid, and uniformly mixing to obtain the conductive ink.
The invention finally protects the application of the conductive ink for vitamin detection, which is used for printing test paper electrodes for detecting one or more vitamins.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention prepares the conductive ink which has electrochemical response to one or more vitamins by organically combining the composite carbon material and the related metal material, and prints the test paper electrode by combining the silk screen printing technology.
2. The method has simple process and low repeated difficulty, can be matched with a mature screen printing technology to conveniently prepare the working electrode with uniform height in large scale, and solves the problem that the glassy carbon electrode used in the existing electrochemical detection means needs to be polished.
Drawings
FIG. 1 is a graph showing the linear relationship of vitamin C detection using the conductive ink prepared in example 1.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The embodiment discloses a conductive ink for vitamin detection, which comprises the following components in percentage by weight: 10% of resin, 28% of conductive slurry, 60% of solvent, 0.5% of thickening agent and 1.5% of defoaming agent; the conductive slurry contains conductive particles, and the weight of the conductive particles accounts for 92% of the total weight of the conductive slurry.
Wherein: the resin is selected from one or more of methyl phenyl silicone resin, epoxy resin and carboxymethyl cellulose; the conductive particles are selected from one or more of graphite, carbon powder, graphene, carbon nano tubes, fullerene, functionalized graphene, functionalized carbon nano tubes, gold powder, bismuth nitrate and platinum powder; the solvent is selected from one or more of polybutadiene glycol, ethylene glycol monoethyl ether, ethanol, isopropanol, dimethylformamide, propylene glycol, purified water, polyvinylpyrrolidone, DMF and cyclohexanone; the antifoaming agent is one or more of Tween-20, Triton-100 or sodium dodecyl sulfate; the thickener is at least one of glycerol or oleic acid, terpineol and methylcellulose.
The embodiment discloses a preparation method of conductive ink for vitamin detection, which comprises the following steps:
(1) weighing the raw materials according to the formula percentage, grinding the conductive particles, and sieving the ground conductive particles with a 400-mesh sieve for later use;
(2) placing the sieved conductive particles in a muffle furnace at the temperature of 400-700 ℃, sintering for 30-100min, taking out and naturally cooling to obtain sintered particles;
(3) adding the sintered particles into an ethanol solution, uniformly mixing by ultrasonic waves, putting the mixed suspension into a rotary evaporator, and evaporating the ethanol solution to obtain viscous slurry to obtain conductive slurry;
(4) and adding resin, a solvent, a defoaming agent and a thickening agent into the conductive liquid, and uniformly mixing to obtain the conductive ink.
Example 2
The mixture ratio and the preparation method of the embodiment are basically the same as those of the embodiment 1, except that: the conductive ink comprises the following components in percentage by weight: 20% of resin, 20% of conductive slurry, 56% of solvent, 1% of thickening agent and 3% of defoaming agent; the conductive slurry contains resin, and the weight of the resin accounts for 95% of the total weight of the conductive slurry.
Example 3
The mixture ratio and the preparation method of the embodiment are basically the same as those of the embodiment 1, except that: the conductive ink comprises the following components in percentage by weight: 30% of resin, 12% of conductive slurry, 52% of solvent, 1% of thickening agent and 5% of defoaming agent; the conductive slurry contains resin, and the weight of the resin accounts for 98% of the total weight of the conductive slurry.
Vitamin detection method
(1) The conductive ink printing working electrode prepared by the preparation method described in the above example was used.
(2) Taking 50 mu L of the solution to be detected, adding 100 mu L of corresponding vitamin B1 releasing agent, uniformly mixing, and then taking 50 mu L of mixed liquid to be dripped on a working electrode printed by the conductive ink;
(3) inserting the test paper electrode into corresponding vitamin detector, and accurately removing vitamin C standard solution V by using a micropipettor in sequence1mL、V2mL、V3mL、V4mL、V5mL、V6mL、V7And mL, adding into the mixed solution respectively. Sequentially measuring the corresponding peak current as i by a vitamin detectorP1、iP2、iP3、iP4、iP5、iP6、iP7C is obtained by calculating the concentration of the mixed solution changed after each addition of the vitamin C standard solution1、c2、c3、c4、c5、c6、c7Seven concentration values corresponding to the peak current. The peak current value iPAnd performing minimum linear fitting with the corresponding concentration value c to obtain a linear relation: i.e. iPK + c + b. Wherein k isLinear coefficient, b is the intercept. Therefore, the concentration value of the vitamin C in the blood sample can be obtained by calculating b; the concentration value of vitamin C in the blood sample is equal to 3 b. (FIG. 1 is a linear relationship diagram of vitamin C detection obtained according to the above-described method using the conductive ink prepared in example 1.)
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
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