阅读说明：本技术 一种烤烟调制定色剂及其制备方法和应用 (Cured tobacco modulation fixative and preparation method and application thereof ) 是由 殷全玉 李晓龙 王新发 张明月 王景 云菲 刘国顺 于 2020-12-03 设计创作，主要内容包括：本发明公开一种烤烟调制定色剂及其制备和使用方法。该烤烟调制定色剂由抗氧化剂、增效剂和表面活性剂组成,在烤烟调制的定色阶段使用。其中,天然抗氧化剂维生素C和维生素E可以有效阻止因烟草调制过程中酚类物质过度氧化而产生的杂色。柠檬酸是抗氧化剂的增效剂,可以显著提高抗氧化剂作用效果。十二烷基硫酸钠是表面活性剂,可以增加烟叶组织结构通透性,一方面是促进抗氧化剂和增效剂进入烟叶细胞组织内部发生作用,另一方面是促进烟叶细胞组织中的水分快速排出来,阻止烟草调制过程因水分排出不及时的酚类物质过度氧化,将黄色固定下来。(The invention discloses a cured tobacco modulation fixative and a preparation and use method thereof. The cured tobacco blending color fixative consists of an antioxidant, a synergist and a surfactant and is used in the color fixing stage of cured tobacco blending. Wherein, the natural antioxidants vitamin C and vitamin E can effectively prevent the variegated color generated by the over-oxidation of phenolic substances in the tobacco preparation process. Citric acid is a synergist of the antioxidant, and can obviously improve the effect of the antioxidant. The sodium dodecyl sulfate is a surfactant, can increase the permeability of the tobacco leaf tissue structure, on one hand, promotes the antioxidant and the synergist to enter the inside of the tobacco leaf cell tissue to take effect, on the other hand, promotes the moisture in the tobacco leaf cell tissue to be quickly discharged, prevents the phenol substances from being excessively oxidized due to untimely discharge of the moisture in the tobacco preparation process, and fixes the yellow color.)

1. The cured tobacco blending fixative is characterized in that raw materials comprise a surfactant, an antioxidant and a synergist; the surfactant is sodium dodecyl sulfate; the synergist is citric acid; the antioxidant is one or more of vitamin C, vitamin E and sodium sulfite.

2. The cured tobacco concoction fixative according to claim 1, which is mainly composed of the following raw materials in concentration;

50g/L of surfactant; 10-56 g/L of antioxidant; 16-28 g/L of synergist.

3. The cured tobacco concoction fixative according to claim 2, which is mainly composed of the following raw materials in concentration;

50g/L of sodium dodecyl sulfate; 8-14 g/L of vitamin C; 8-14 g/L of vitamin E; 16-28 g/L of sodium sulfite; 16-28 g/L of citric acid.

4. The cured tobacco concoction fixative according to claim 3, which is mainly composed of the following raw materials in concentration;

50g/L of sodium dodecyl sulfate; 10-12 g/L of vitamin C; vitamin E10-12 g/L; 18-26 g/L of sodium sulfite; 18-26 g/L of citric acid.

5. The cured tobacco concoction fixative according to claim 4, which is mainly composed of the following raw materials in concentration;

50g/L of sodium dodecyl sulfate; 10-12 g/L of vitamin C; vitamin E10-12 g/L; 20-24 g/L of sodium sulfite; 20-24 g/L of citric acid.

6. The cured tobacco concoction fixative according to claim 5, which is mainly composed of the following raw materials in concentration;

50g/L of sodium dodecyl sulfate; vitamin C10 g/L; vitamin E10 g/L; 20g/L of sodium sulfite; citric acid 20 g/L.

7. The preparation method of the cured tobacco prepared fixative as claimed in any one of claims 1-6, characterized by comprising the following steps:

(1) dissolving sodium dodecyl sulfate in water, stirring until the sodium dodecyl sulfate is completely dissolved, and preparing liquid a;

(2) dissolving vitamin E in ethanol, stirring until the vitamin E is completely dissolved, and preparing liquid b;

(3) dissolving citric acid in water, stirring until the citric acid is completely dissolved, and preparing liquid c;

(4) dissolving vitamin C in water, stirring until the vitamin C is completely dissolved, and preparing liquid d;

(5) dissolving sodium sulfite in water, stirring until the sodium sulfite is completely dissolved, and preparing into liquid e;

(6) and pouring the liquid a, the liquid b, the liquid c, the liquid d and the liquid e into the same container, adding the residual water, and uniformly stirring to ensure that the components reach the required concentration, thereby obtaining the cured tobacco blending fixative.

8. The cured tobacco modulation fixing agent of any one of claims 1-6 is applied in the cured tobacco modulation fixing stage.

9. Use according to claim 8, characterized in that the steps are as follows:

1) diluting the prepared fixative solvent by using tap water or distilled water for later use to obtain fixative diluent;

2) when the tobacco leaf modulation is in a large moisture removal stage, placing the fixing agent diluent prepared in the step 1) at a circulating fan, opening the fan to atomize the fixing agent diluent, and then sending the atomized fixing agent diluent into a tobacco curing house;

3) and monitoring the residual amount of the fixing agent diluent, replenishing in time, and stopping using the fixing agent when the temperature in the curing barn exceeds 54 ℃.

10. The use of claim 9, wherein the dilution factor of the fixer diluent of step 1) is 20 times.

Technical Field

The invention relates to the technical field of tobacco production and cigarette manufacturing, in particular to a preparation for preventing tobacco leaves from being fixed in yellow and easily producing variegated colors, ash and even black lees in the tobacco blending process, and a preparation and use method of the preparation.

Background

The curing process of the flue-cured tobacco can be divided into three stages, namely a yellowing stage, a color fixing stage and a tendon drying stage. The main chemical reaction in the yellowing stage is the degradation of protein, starch and chlorophyll in tobacco leaves, and the tobacco leaves are changed from green to yellow; the fixation period is that the moisture in the tobacco leaves is quickly discharged by quickly raising the temperature in the curing barn, so that the leaves are dried, the yellow color is fixed, the cell membranes of the leaves are broken at the stage, the life is lost, the polyphenol oxidase is released, if the moisture in the leaf tissues can not be quickly discharged, the polyphenol oxidase can quickly act, the polyphenol and the protein containing the polyphenol structure are oxidized, brown pigment is generated, and the originally yellow tobacco leaves generate mixed color, ash hanging and even black lees. In the production process, some excellent flue-cured tobacco varieties have rich contents, much oil and strong moisture holding capacity, and the tobacco leaves are difficult to dehydrate in the color fixing period, so that the variegated tobacco is generated. The excessive nutrition of tobacco plants caused by the higher usage amount of the nitrogen fertilizer, the produced large and deep tobacco leaves, the tobacco leaves turning green after rain and the tobacco leaves harvested in the autumn, especially the upper tobacco leaves, have rich polyphenol substances, the polyphenol oxidase activity is higher, the color fixation during the modulation period is difficult, and the phenomena of impurity color and ash hanging are easy to occur. The research and popularization of the substances which can inhibit the generation of the variegated tobacco leaves caused by the excessive oxidation of the polyphenols in the curing process of the flue-cured tobacco are urgently needed in production,

although the research of inhibiting the activity of polyphenol oxidase in tobacco leaves by using substances such as vitamin C, vitamin E, sodium sulfite, organic acid and the like and preventing excessive browning reaction caused by improper baking is more, the tobacco leaves still have biological activity in the yellowing stage of tobacco leaf modulation in the using period, and polyphenol oxidase and action substrates thereof are separated in different areas by cell membranes, so that the browning reaction cannot occur. The antioxidant substances used in advance do not function in the yellowing stage, but rather are lost partly due to the higher temperature in the baking room.

It has been studied to formulate an antioxidant into an aqueous solution and then use it by spraying or soaking. The surface of the tobacco leaves is covered with a dense and impermeable cuticle, and the antioxidant is applied to the tobacco leaves in the form of a water preparation, so that the antioxidant is not beneficial to entering the inside of cells to play a role.

The color fixing period is a key node for flue-cured tobacco modulation, and whether the moisture in the tobacco leaves can be quickly discharged is a key node in the color fixing period. In the color fixing period, some tobacco leaves have very strong water holding capacity, the water in cells cannot be synchronously and quickly discharged although the temperature in a curing barn is raised, the high-temperature and high-humidity environment provides long acting time for polyphenol oxidase in the cells, and polyphenol substances are excessively oxidized, so that variegated smoke, ash hanging smoke and even black distilled grain smoke are generated.

Disclosure of Invention

In order to solve the problem that polyphenol substances in a color fixing period are easily excessively oxidized in the current tobacco curing process, so that brown pigment is generated, and the quality of tobacco leaves is reduced, the invention provides a cured tobacco curing fixative, a preparation method and a use method thereof, and the adopted technical scheme is as follows:

a cured tobacco modulation fixative, the fixative raw materials include surfactant active, antioxidant and synergist; the surfactant is sodium dodecyl sulfate; the synergist is citric acid; the antioxidant is one or more of vitamin C, vitamin E and sodium sulfite.

Preferably, the fixative agent is mainly composed of the following raw materials in concentration;

50g/L of surfactant; 10-56 g/L of antioxidant; 16-28 g/L of synergist.

More preferably, the fixative agent consists essentially of the following concentrations of raw materials;

50g/L of sodium dodecyl sulfate; 8-14 g/L of vitamin C; 8-14 g/L of vitamin E; 16-28 g/L of sodium sulfite; 16-28 g/L of citric acid.

More preferably, the fixative agent consists essentially of the following concentrations of raw materials;

50g/L of sodium dodecyl sulfate; 10-12 g/L of vitamin C; vitamin E10-12 g/L; 18-26 g/L of sodium sulfite; 18-26 g/L of citric acid.

More preferably, the fixative agent consists essentially of the following concentrations of raw materials;

50g/L of sodium dodecyl sulfate; 10-12 g/L of vitamin C; vitamin E10-12 g/L; 20-24 g/L of sodium sulfite; 20-24 g/L of citric acid.

More preferably, the fixative agent consists essentially of the following concentrations of raw materials;

50g/L of sodium dodecyl sulfate; vitamin C10 g/L; vitamin E10 g/L; 20g/L of sodium sulfite; citric acid 20 g/L.

The invention also aims to provide a preparation method of the cured tobacco prepared fixative, which comprises the following steps:

dissolving sodium dodecyl sulfate in water, stirring until the sodium dodecyl sulfate is completely dissolved, and preparing liquid a;

1) dissolving vitamin E in ethanol, stirring until the vitamin E is completely dissolved, and preparing liquid b;

2) dissolving citric acid in water, stirring until the citric acid is completely dissolved, and preparing liquid c;

3) dissolving vitamin C in water, stirring until the vitamin C is completely dissolved, and preparing liquid d;

4) dissolving sodium sulfite in water, stirring until the sodium sulfite is completely dissolved, and preparing into liquid e;

5) and pouring the liquid a, the liquid b, the liquid c, the liquid d and the liquid e into the same container, adding the residual water, and uniformly stirring to ensure that the components reach the required concentration, thereby obtaining the cured tobacco blending fixative.

The cured tobacco modulation fixing agent is applied in the cured tobacco modulation fixing stage.

The application steps are as follows:

1) diluting the prepared fixative solvent by using tap water or distilled water for later use to obtain fixative diluent;

2) when the tobacco leaf modulation is in a large moisture removal stage, placing the fixing agent diluent prepared in the step 1) at a circulating fan, opening the fan to atomize the fixing agent diluent, and then sending the atomized fixing agent diluent into a tobacco curing house;

3) and monitoring the residual amount of the fixing agent diluent, replenishing in time, and stopping using the fixing agent when the temperature in the curing barn exceeds 54 ℃.

Preferably, the dilution factor of the fixer diluent in the step 1) is 20 times.

The flue-cured tobacco suitable for the flue-cured tobacco modulation fixative is preferably selected from the group consisting of large and deep-thickness tobacco leaves with surplus nutrition, flue-cured tobacco leaves harvested after the beginning of autumn, upper leaves, flue-cured tobacco varieties with strong water-holding capacity and other tobacco leaves with large potential black-cured risks.

The technical principle of the invention is as follows:

vitamin C, vitamin E and sodium sulfite antioxidants in the cured tobacco modulation fixative can effectively prevent the variegated color generated by over-oxidation of phenolic substances in the tobacco modulation process. Citric acid is a synergist of the antioxidant, and can obviously improve the effect of the antioxidant. The sodium dodecyl sulfate is a surfactant, can increase the permeability of the tobacco leaf tissue structure, on one hand, promotes the antioxidant and the synergist to enter the inside of the tobacco leaf cell tissue to take effect, on the other hand, promotes the moisture in the tobacco leaf cell tissue to be quickly discharged, prevents the phenol substances from being excessively oxidized due to untimely discharge of the moisture in the tobacco preparation process, and fixes the yellow color.

Compared with the prior art, the invention has the following beneficial effects:

according to the invention, the antioxidant is dissolved in the surfactant lauryl sodium sulfate solution for use, and the lauryl sodium sulfate and the cuticle on the surface of the tobacco leaf act to increase the permeability of the tobacco leaf, so that on one hand, the antioxidant is promoted to enter more cells to inhibit the activity of polyphenol oxidase, on the other hand, the moisture in the cells is promoted to be discharged more quickly, the action time of polyphenol oxidase is shortened, and the yellow color of the tobacco leaf is fixed.

The cured tobacco modulation fixative consists of an antioxidant, a surfactant and a synergist 3. The surfactant sodium dodecyl sulfate can increase the permeability of the cuticle of the tobacco leaf, promote the antioxidant to enter the cell more, and inhibit the activity of polyphenol oxidase.

The sodium dodecyl sulfate acts on the cuticle on the surface of the tobacco leaves to increase the permeability of the tobacco leaves, promote the moisture in the tobacco leaves to be discharged more quickly, reduce the acting time of polyphenol oxidase and fix the yellow color of the tobacco leaves. Meanwhile, the tobacco leaves are dried more quickly, and the cost is reduced.

Compared with the prior art, the method for preparing the cured tobacco in the yellowing period has the advantages that the method is used in the curing period of curing the cured tobacco, the influence of the curing agent on the substance conversion in the tobacco in the yellowing period is reduced, the oxidation consumption is reduced, and the effect is more obvious.

And (3) directly sending the diluted fixative into the tobacco curing room by using a circulating fan in the fixation period of curing tobacco modulation, and compared with the existing spraying or soaking use technology, the method does not need to additionally increase labor.

Detailed Description

The present invention will be further described with reference to the following specific examples, but the present invention is not limited to these examples.

The materials, reagents, methods, apparatuses, etc. used in the following examples are, without being specifically described, all materials, reagents, methods, apparatuses, etc. which are conventional in the art. Those skilled in the art are commercially available.

Example 1

The embodiment provides a cured tobacco prepared fixative solution, wherein a carrier of the solution is distilled water or tap water, and the solution comprises the following components in concentration:

50g/L of lauryl sodium sulfate, 10g/L of vitamin C, 10g/L of vitamin E, 10g g/L of sodium sulfite and 20g/L of citric acid.

The preparation process of the cured tobacco prepared fixative solution comprises the following steps:

1) dissolving 50g of sodium dodecyl sulfate in 500mL of water, and stirring until the sodium dodecyl sulfate is completely dissolved to prepare a liquid a;

2) dissolving 10g of vitamin E in a small amount of ethanol, stirring until the vitamin E is completely dissolved, and preparing liquid b;

3) dissolving 20g of citric acid in 100mL of water, and stirring until the citric acid is completely dissolved to prepare liquid c;

4) dissolving 10g of vitamin C in 100mL of water, stirring until the vitamin C is completely dissolved, and preparing liquid d;

5) dissolving 20g of sodium sulfite in 100mL of water, stirring until the sodium sulfite is completely dissolved, and preparing into liquid e;

6) and pouring the liquid a, the liquid b, the liquid c, the liquid d and the liquid e into a 1L volumetric flask, adding water to a constant volume to reach a scale, and fully shaking up to obtain the cured tobacco blending fixative.

The fixative solution is used in the process of moisture removal of tobacco leaves, and the specific use process is as follows:

1) the fixative is easy to dilute by 20 times for standby;

2) placing the diluted prepared fixing agent in the step 1) at a circulating fan, turning on the fan, atomizing the fixing agent, conveying the atomized fixing agent into a tobacco curing barn by using the circulating fan, and rapidly circulating;

3) detecting the residual amount of the fixing agent solution and replenishing liquid in time;

4) when the temperature of the curing barn rises to above 54 ℃, the curing barn is stopped.

An equal amount of tap water was used as a control during the test.

Example 2

The embodiment provides a cured tobacco prepared fixative solution, wherein a carrier of the solution is distilled water or tap water, and the solution comprises the following components in concentration:

50g/L of lauryl sodium sulfate, 8g/L of vitamin C, 8g/L of vitamin E, 16g/L of sodium sulfite and 16g/L of citric acid.

The preparation method and the use method of the cured tobacco concoction fixative are the same as those in the example 1, and the contrast is the same amount of tap water.

Example 3

The embodiment provides a cured tobacco prepared fixative solution, wherein a carrier of the solution is distilled water or tap water, and the solution comprises the following components in concentration:

50g/L of lauryl sodium sulfate, 14g/L of vitamin C, 14/14 g/L of vitamin E, 28g/L of sodium sulfite and 26g/L of citric acid.

The preparation method and the use method of the cured tobacco concoction fixative are the same as those in the example 1, and the contrast is the same amount of tap water.

Example 4

The embodiment provides a cured tobacco prepared fixative solution, wherein a carrier of the solution is distilled water or tap water, and the solution comprises the following components in concentration:

50g/L of lauryl sodium sulfate, 10g/L of vitamin C, 20g/L of sodium sulfite and 20g/L of citric acid.

The preparation method and the use method of the cured tobacco concoction fixative are the same as those in the example 1, and the contrast is the same amount of tap water.

Example 5

The embodiment provides a cured tobacco prepared fixative solution, wherein a carrier of the solution is distilled water or tap water, and the solution comprises the following components in concentration:

50g/L of lauryl sodium sulfate, 10g/L of vitamin C and 20g/L of citric acid.

The preparation method and the use method of the cured tobacco concoction fixative are the same as those in the example 1, and the contrast is the same amount of tap water.

Example 6

The embodiment provides a cured tobacco prepared fixative solution, wherein a carrier of the solution is distilled water or tap water, and the solution comprises the following components in concentration:

50g/L of lauryl sodium sulfate, 10g/L of vitamin E and 20g/L of citric acid.

The preparation method and the use method of the cured tobacco concoction fixative are the same as those in the example 1, and the contrast is the same amount of tap water.

Example 7

The embodiment provides a cured tobacco prepared fixative solution, wherein a carrier of the solution is distilled water or tap water, and the solution comprises the following components in concentration:

50g/L of sodium dodecyl sulfate, 20g/L of sodium sulfite and 20g/L of citric acid.

The preparation method and the use method of the cured tobacco concoction fixative are the same as those in the example 1, and the contrast is the same amount of tap water.

Example 8

In order to verify the technical effect of the prepared fixative for flue-cured tobacco, the color, the grade and the residual amount of polyphenols of the tobacco leaves treated by the fixative prepared in the embodiments 1 to 7 are measured. The results are shown in tables 1 and 2.

TABLE 1 Effect of the treatments on the color and grade of the cured tobacco leaves

Treatment of	Orange tobacco proportion/%)	Mottled tobacco proportion/%)	First-class smoke proportion/%)	Lower equi-smoke proportion/%)
					Example 1	43.7	21.7	39.1	27.8
Example 2	38.9	23.4	36.8	28.3
					Example 3	37.4	30.7	35.7	30.4
Example 4	43.5	26.8	38.8	25.6
					Example 5	36.8	33.2	33.9	28.4
Example 6	32.6	30.4	30.4	28.9
					Example 7	38.2	31.6	35.9	26.8
Control	29.7	40.5	28.7	31.5

TABLE 2 Effect of the treatments on the Polyphenol content of the cured tobacco/%)

From the above results, it is clear that the yellow smoke proportion and the first-class smoke proportion are significantly increased, and the variegated smoke proportion and the lower-class smoke proportion are significantly decreased after the treatment and the modulation. The polyphenol content of tobacco leaves B2F and C3F is analyzed, and the reduction of polyphenol substances is effectively prevented by each treatment.

Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.