阅读说明：本技术 植物中流感样病毒颗粒的产生 (Production of influenza-like virus particles in plants ) 是由 曼侬·库特里 马克-安德烈·蒂奥斯特 路易斯-菲利普·维泽纳 于 2014-03-28 设计创作，主要内容包括：提供了在植物中产生包含修饰的血凝素的病毒样颗粒(VLP)的方法。所述方法包括将包含调控区的核酸引入所述植物或所述植物的部分,所述调控区在植物中有活性并且与编码修饰的流感血凝素(HA)蛋白质的核苷酸序列可操作地连接,所述修饰的HA蛋白包含修饰的蛋白水解环。在允许所述核酸表达的条件下培养所述植物或所述植物的部分后,从而产生所述VLP。所述修饰的蛋白水解环可包含呈现出减少或废除的蛋白酶切割的一个或多个蛋白酶切割位点。编码所述HA的所述核苷酸序列可选自：B HA、C、H1、H2、H3、H4、H5、H6、H7、H8、H9、H10、H11、H12、H13、H14、H15和H16。也描述了通过所述方法产生的病毒样颗粒(VLP),以及表达所述VLP的植物。所述病毒样颗粒(VLP)可包含植物-特异性N-聚糖,或修饰的N-聚糖。(Methods of producing virus-like particles (VLPs) comprising modified hemagglutinin in a plant are provided. The method comprises introducing into the plant or a part of the plant a nucleic acid comprising a regulatory region active in the plant and operably linked to a nucleotide sequence encoding a modified influenza Hemagglutinin (HA) protein, the modified HA protein comprising a modified proteolytic loop. (ii) growing said plant or part of said plant under conditions which allow expression of said nucleic acid, thereby producing said VLP. The modified proteolytic loop may comprise one or more protease cleavage sites that exhibit reduced or abolished protease cleavage. The nucleotide sequence encoding the HA may be selected from: b HA, C, H1, H2, H3, H4, H5, H6, H7, H8, H9, H10, H11, H12, H13, H14, H15 and H16. Virus-like particles (VLPs) produced by the method, and plants expressing the VLPs, are also described. The virus-like particle (VLP) may comprise plant-specific N-glycans, or modified N-glycans.)