阅读说明：本技术 一种从牛血清中分离牛血清白蛋白的制备方法及装置 (Preparation method and device for separating bovine serum albumin from bovine serum ) 是由 李佳亮 孙浩 于 2020-12-15 设计创作，主要内容包括：本发明属于牛血清白蛋白制作设备技术领域,具体公开了一种从牛血清中分离牛血清白蛋白的制备方法,包括以下步骤：取适量新鲜的牛血放入牛血清白蛋白制作一体机中的预处理室中,加压使得牛血中红细胞破碎后,离心分离得到上清液；得到的上清液通入牛血清白蛋白制作一体机中的反应室中,加入沉淀剂分离后得到牛血清；牛血清通入牛血清白蛋白制作一体机中振动透析分离室中,向牛血清中加入PBS溶液,混匀后进行振动透析分离得到溶液；溶液通入牛血清白蛋白制作一体机中的交换室中通过阴离子交换法的到精纯的牛血清白蛋白；精纯的牛血清白蛋白通入牛血清白蛋白制作一体机中的冻干室中,得到牛血清白蛋白粉。(The invention belongs to the technical field of bovine serum albumin manufacturing equipment, and particularly discloses a preparation method for separating bovine serum albumin from bovine serum, which comprises the following steps: taking a proper amount of fresh bovine blood, putting the fresh bovine blood into a pretreatment chamber in a bovine serum albumin manufacturing all-in-one machine, pressurizing to break red blood cells in the bovine blood, and performing centrifugal separation to obtain supernatant; introducing the obtained supernatant into a reaction chamber in a bovine serum albumin manufacturing all-in-one machine, adding a precipitator, and separating to obtain bovine serum; introducing bovine serum into a vibration dialysis separation chamber in a bovine serum albumin manufacturing all-in-one machine, adding a PBS (phosphate buffer solution) solution into the bovine serum, uniformly mixing, and performing vibration dialysis separation to obtain a solution; introducing the solution into an exchange chamber in the integrated bovine serum albumin manufacturing machine to obtain pure bovine serum albumin by an anion exchange method; introducing the pure bovine serum albumin into a freeze-drying chamber in the bovine serum albumin manufacturing all-in-one machine to obtain the bovine serum albumin powder.)

1. A preparation method for separating bovine serum albumin from bovine serum is characterized by comprising the following steps:

s1, taking a proper amount of fresh bovine blood, putting the fresh bovine blood into a pretreatment chamber in the bovine serum albumin manufacturing all-in-one machine, pressurizing to break red blood cells in the bovine blood, and centrifuging to obtain supernatant;

s2, introducing the supernatant obtained in the step S1 into a reaction chamber in a bovine serum albumin making all-in-one machine, and adding a precipitator for separation to obtain bovine serum;

s3, introducing the bovine serum in the step S2 into a vibration dialysis separation chamber in a bovine serum albumin manufacturing all-in-one machine, adding a PBS (phosphate buffer solution) solution into the bovine serum, uniformly mixing, and performing vibration dialysis separation to obtain a solution;

s4, introducing the solution obtained in the step S3 into an exchange chamber in a bovine serum albumin making all-in-one machine, and obtaining pure bovine serum albumin through an anion exchange method;

s5, introducing the pure bovine serum albumin obtained in the step S4 into a freeze-drying chamber of the bovine serum albumin making all-in-one machine, and freeze-drying to obtain the bovine serum albumin powder.

2. The method according to claim 1, wherein the bovine serum albumin is isolated from bovine serum, and the method comprises the steps of: the pressure value in the step S1 is 10 standard atmospheres.

3. The method according to claim 1, wherein the bovine serum albumin is isolated from bovine serum, and the method comprises the steps of: and the precipitator in the step S2 is calcium chloride powder, and the calcium chloride powder and the supernatant are mixed and centrifugally separated to obtain bovine serum.

4. The apparatus according to any one of claims 1 to 4, wherein the apparatus comprises: the device is bovine serum albumin preparation all-in-one, including pre-treatment chamber (1), reaction chamber (2), vibration dialysis separator room (3), exchange chamber (4) and freeze-drying chamber (5), be provided with preprocessing device (6) in pre-treatment chamber (1), be provided with reaction unit (7) in reaction chamber (2), be provided with vibration dialysis separator device (8) in vibration dialysis separator room (3), exchange chamber (4) are connected with freeze-drying chamber (5).

5. The apparatus according to claim 4, wherein the apparatus comprises: preprocessing device (6) include centrifuge (9), centrifuge (9) set up in preprocessing chamber (1), centrifuge (9) bottom is provided with discharge gate (10), preprocessing chamber (1) top is provided with sealed apron (11), be provided with pressure sensor (12) and elevator motor (13) on the sealed apron (11), elevator rod (14) bottom of elevator motor (13) is provided with suction tube (15), suction tube (15) are through suction pump (16) connection reaction chamber (2), preprocessing chamber (1) one side is provided with pressure device (17), be provided with infrared sensor (18) on suction tube (15).

6. The apparatus according to claim 4, wherein the apparatus comprises: reaction unit (7) include agitator motor (19), agitator motor (19) set up on reaction chamber (2) top, agitator rod (20) of agitator motor (19) set up in reaction chamber (2), reaction chamber (2) top one side is provided with adds feed box (21), be provided with solenoid valve (23) on charge door (22) of adding feed box (21) bottom, vibration dialysis separation room (3) are connected through connecting pipe (24) in reaction chamber (2) bottom, be provided with filter layer (25) on connecting pipe (24), also be provided with solenoid valve (23) on connecting pipe (24).

7. The apparatus according to claim 4, wherein the apparatus comprises: vibration dialysis separator (8) include vibrating motor (26), vibrating motor (26) are installed in vibration dialysis separator (3) bottom, vibration dialysis separator (3) top is provided with pneumatic cylinder (27), hydraulic stem (28) bottom of pneumatic cylinder (27) is provided with clamp plate (29), clamp plate (29) and vibration dialysis separator (3) sealed sliding connection.

8. The apparatus according to claim 4, wherein the apparatus comprises: the utility model discloses a vibration dialysis separation chamber, including vibration dialysis separation chamber (3), vibration dialysis separation chamber (3) are still provided with medicine box (30) on one side of vibration dialysis separation chamber (3), vibration dialysis separation chamber (3) are connected in play medicine mouth (31) of medicine box (30), also be provided with solenoid valve (23) on play medicine mouth (31), vibration dialysis separation chamber (3) bottom is provided with dialysis layer (32).

Technical Field

The invention relates to the field of bovine serum albumin manufacturing equipment, in particular to a preparation method and a device for separating bovine serum albumin from bovine serum.

Background

Most of the existing bovine serum albumin is prepared by separating erythrocytes from plasma and then extracting, but the erythrocytes also contain protein, so that the material is wasted, meanwhile, the existing bovine serum albumin is prepared by more steps and is generally distributed to different devices for preparation and production, so that the materials are required to be continuously transferred by workers, the processing progress and the preparation quality of the bovine serum albumin are delayed, and the preparation method and the device for separating the bovine serum albumin from the bovine serum are designed to solve the problems.

Disclosure of Invention

The present invention aims to provide a method and a device for separating bovine serum albumin from bovine serum, so as to solve the problems in the background art.

In order to achieve the purpose, the invention provides the following technical scheme: a preparation method for separating bovine serum albumin from bovine serum comprises the following steps:

s1, taking a proper amount of fresh bovine blood, putting the fresh bovine blood into a pretreatment chamber in the bovine serum albumin manufacturing all-in-one machine, pressurizing to break red blood cells in the bovine blood, and centrifuging to obtain supernatant;

s2, introducing the supernatant obtained in the step S1 into a reaction chamber in a bovine serum albumin making all-in-one machine, and adding a precipitator for separation to obtain bovine serum;

s3, introducing the bovine serum in the step S2 into a vibration dialysis separation chamber in a bovine serum albumin manufacturing all-in-one machine, adding a PBS (phosphate buffer solution) solution into the bovine serum, uniformly mixing, and performing vibration dialysis separation to obtain a solution;

s4, introducing the solution obtained in the step S3 into an exchange chamber in a bovine serum albumin making all-in-one machine, and obtaining pure bovine serum albumin through an anion exchange method;

s5, introducing the pure bovine serum albumin obtained in the step S4 into a freeze-drying chamber of the bovine serum albumin making all-in-one machine, and freeze-drying to obtain the bovine serum albumin powder.

Preferably, the pressure value in step S1 is 10 standard atmospheres.

Preferably, the precipitant in step S2 is calcium chloride powder, and the calcium chloride powder is mixed with the supernatant and centrifuged to obtain bovine serum.

Preferably, device bovine serum albumin preparation all-in-one, including pre-treatment chamber, reaction chamber, vibration dialysis separator, exchange chamber and freeze-drying chamber, be provided with preprocessing device in the pre-treatment chamber, be provided with reaction unit in the reaction chamber, be provided with vibration dialysis separator in the vibration dialysis separator, the exchange chamber is connected with freeze-drying chamber.

Preferably, preprocessing device includes centrifuge, centrifuge sets up in the precomminution room, the centrifuge bottom is provided with the discharge gate, the precomminution room top is provided with sealed apron, be provided with pressure sensor and elevator motor on the sealed apron, elevator motor's lifter bottom is provided with the suction tube, the suction tube passes through the suction pump and connects the reaction chamber, precomminution room one side is provided with pressure device, be provided with infrared sensor on the suction tube.

Preferably, reaction unit includes agitator motor, agitator motor sets up on the reacting chamber top, agitator motor's puddler sets up in the reacting chamber, reacting chamber top one side is provided with reinforced box, be provided with solenoid valve on the charge door of reinforced box bottom, the vibrating dialysis separation chamber is connected through the connecting pipe to the reacting chamber bottom, be provided with the filter layer on the connecting pipe, also be provided with solenoid valve on the connecting pipe.

Preferably, the vibration dialysis separation device comprises a vibration motor, the vibration motor is installed at the bottom end of the vibration dialysis separation chamber, a hydraulic cylinder is arranged at the top end of the vibration dialysis separation chamber, a pressing plate is arranged at the bottom end of a hydraulic rod of the hydraulic cylinder, and the pressing plate is connected with the vibration dialysis separation chamber in a sealing and sliding mode.

Preferably, a medicine box is further arranged on one side of the vibration dialysis separation chamber, a medicine outlet of the medicine box is connected with the vibration dialysis separation chamber, an electromagnetic valve is also arranged on the medicine outlet, and a dialysis layer is arranged at the bottom end of the vibration dialysis separation chamber.

Compared with the prior art, the invention has the beneficial effects that:

the invention belongs to the technical field of bovine serum albumin manufacturing equipment, and particularly discloses a preparation method and a device for separating bovine serum albumin from bovine serum.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of a pretreatment apparatus of the present invention;

FIG. 3 is a schematic view of a reaction apparatus according to the present invention;

FIG. 4 is a schematic view of a vibratory dialysis separator apparatus of the present invention;

in the figure: 1. a pretreatment chamber; 2. a reaction chamber; 3. a vibration dialysis separation chamber; 4. an exchange chamber; 5. a lyophilization chamber; 6. a pretreatment device; 7. a reaction device; 8. a vibratory dialysis separator; 9. a centrifugal separator; 10. a discharge port; 11. sealing the cover plate; 12. a pressure sensor; 13. a lifting motor; 14. a lifting rod; 15. a suction tube; 16. a suction pump; 17. a pressurizing device; 18. an infrared sensor; 19. a stirring motor; 20. a stirring rod; 21. a feeding box; 22. a feed inlet; 23. an electromagnetic valve; 24. a connecting pipe; 25. a filter layer; 26. a vibration motor; 27. a hydraulic cylinder; 28. a hydraulic lever; 29. pressing a plate; 30. a kit; 31. a medicine outlet; 32. and (6) a dialysis layer.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "vertical", "upper", "lower", "horizontal", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

Referring to fig. 1-4, the present invention provides a technical solution: a preparation method for separating bovine serum albumin from bovine serum comprises the following steps:

s1, taking a proper amount of fresh bovine blood, putting the fresh bovine blood into a pretreatment chamber in the bovine serum albumin manufacturing all-in-one machine, pressurizing to break red blood cells in the bovine blood, and centrifuging to obtain supernatant;

s2, introducing the supernatant obtained in the step S1 into a reaction chamber in a bovine serum albumin making all-in-one machine, and adding a precipitator for separation to obtain bovine serum;

s3, introducing the bovine serum in the step S2 into a vibration dialysis separation chamber in a bovine serum albumin manufacturing all-in-one machine, adding a PBS (phosphate buffer solution) solution into the bovine serum, uniformly mixing, and performing vibration dialysis separation to obtain a solution;

s4, introducing the solution obtained in the step S3 into an exchange chamber in a bovine serum albumin making all-in-one machine, and obtaining pure bovine serum albumin through an anion exchange method;

s5, introducing the pure bovine serum albumin obtained in the step S4 into a freeze-drying chamber of the bovine serum albumin making all-in-one machine, and freeze-drying to obtain the bovine serum albumin powder.

Further, the pressure value in the step S1 is 10 standard atmospheres.

Further, the precipitator in the step S2 is calcium chloride powder, and the calcium chloride powder and the supernatant are mixed and centrifuged to obtain bovine serum.

Further, device bovine serum albumin preparation all-in-one, including pre-treatment chamber 1, reaction chamber 2, vibration dialysis separator chamber 3, exchange chamber 4 and freeze-drying chamber 5, be provided with preprocessing device 6 in the pre-treatment chamber 1, be provided with reaction unit 7 in the reaction chamber 2, be provided with vibration dialysis separator device 8 in the vibration dialysis separator chamber 3, exchange chamber 4 is connected with freeze-drying chamber 5.

Further, preprocessing device 6 includes centrifuge 9, centrifuge 9 sets up in preprocessing chamber 1, centrifuge 9 bottom is provided with discharge gate 10, preprocessing chamber 1 top is provided with sealed apron 11, be provided with pressure sensor 12 and elevator motor 13 on the sealed apron 11, elevator motor 13's lifter 14 bottom is provided with suction tube 15, suction tube 15 passes through suction pump 16 and connects reaction chamber 2, preprocessing chamber 1 one side is provided with pressure device 17, be provided with infrared sensor 18 on the suction tube 15.

Further, reaction unit 7 includes agitator motor 19, agitator motor 19 sets up on 2 tops of reacting chamber, agitator motor 19's puddler 20 sets up in reacting chamber 2, 2 top one sides of reacting chamber are provided with reinforced box 21, be provided with solenoid valve 23 on the charge door 22 of reinforced box 21 bottom, 2 bottoms of reacting chamber are passed through connecting pipe 24 and are connected vibration dialysis separation chamber 3, be provided with filter layer 25 on the connecting pipe 24, also be provided with solenoid valve 23 on the connecting pipe 24.

Further, the vibration dialysis separation device 8 comprises a vibration motor 26, the vibration motor 26 is installed at the bottom end of the vibration dialysis separation chamber 3, a hydraulic cylinder 27 is arranged at the top end of the vibration dialysis separation chamber 3, a pressing plate 29 is arranged at the bottom end of a hydraulic rod 28 of the hydraulic cylinder 27, and the pressing plate 29 is connected with the vibration dialysis separation chamber 3 in a sealing and sliding manner.

Further, a medicine box 30 is further arranged on one side of the vibration dialysis separation chamber 3, a medicine outlet 31 of the medicine box 30 is connected with the vibration dialysis separation chamber 3, an electromagnetic valve 23 is also arranged on the medicine outlet 31, and a dialysis layer 32 is arranged at the bottom end of the vibration dialysis separation chamber 3.

The working principle is as follows: the invention relates to a preparation method and apparatus for separating bovine serum albumin from bovine serum, when using, add bovine serum into centrifuge 9 in the pretreatment chamber 1, then pressurize the pretreatment chamber 1 through the pressure device 17, make the pressure value reach 10 standard atmospheric pressures, guarantee the erythrocyte can break completely, make the protein in the erythrocyte flow out, then work and carry on the centrifugal separation to bovine serum through the centrifuge 9, stratify after stewing, work and drive the lifting lever 14 to descend through the elevator motor 13, make the suction pipe 15 insert in the supernatant, then the suction pump 16 works and draws the supernatant into the reaction chamber 2, the infrared sensor 18 set in the suction pipe 15 can distinguish the stratification of the supernatant, prevent the suction pipe 15 from drawing excessively;

introducing the mixture into a reaction chamber 3, opening an electromagnetic valve 23 on a feeding port 22 arranged on a feeding box 21, adding a precipitator into the supernatant, driving a stirring rod 20 to rotate through the work of a stirring motor 19 to stir and mix the supernatant, obtaining bovine serum after the mixture passes through a filter layer 25, and introducing the bovine serum into a vibration dialysis separation chamber 3;

PBS solution is added to medicine box 30 in the vibration dialysis isolation chamber 3, drive the vibration of vibration dialysis isolation chamber 3 through vibrating motor 26 work, make ox blood serum and PBS solution mix, then dialyse mixed liquid through dialysis layer 32, the pneumatic cylinder 27 work that the cooperation set up promotes hydraulic stem 28 and clamp plate 29 and descends and carry out the filter-pressing to mixed liquid, improve dialysis efficiency, the solution gets into exchange chamber 4 and obtains pure bovine serum albumin through the anion exchange method after the dialysis, then obtain ox blood serum albumin powder through freeze-drying chamber 5.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.