阅读说明：本技术 一种核酸适配体光波导传感器及应用其的检测方法 (Nucleic acid adapter optical waveguide sensor and detection method using same ) 是由 娄新徽 赵家兴 陆张伟 王朔 于 2020-03-16 设计创作，主要内容包括：本发明涉及一种具有靶标原位富集和纯化功能的核酸适配体光波导传感器及基于小分子靶标和与核酸适配体互补短链DNA与偶联在光纤表面的核酸适配体竞争性结合,实现对小分子靶标的定量检测的方法。通过将光波导传感器的二氧化硅纤维进行脱氧核糖核酸适配体和固相微萃取层修饰,实现核酸适配体与靶标的特异性结合与靶标在原位高效富集纯化同步进行。无需任何基于酶的信号放大反应,对多种小分子靶标实现具有超高灵敏度和超高特异性的快速检测。检出限非常低,有优越普适性。可用于复杂样本中靶标直接检测,仅需对液体样品进行稀释,无需样品前处理,检测灵敏度均满足食品和环境中对各靶标的限量标准。检测时间短,传感器再生速度快,可重复使用。(The invention relates to a nucleic acid aptamer optical waveguide sensor with target in-situ enrichment and purification functions and a method for realizing quantitative detection of a small molecular target based on competitive combination of the small molecular target, short-chain DNA complementary with the nucleic acid aptamer and the nucleic acid aptamer coupled on the surface of an optical fiber. The specific combination of the aptamer and the target and the in-situ efficient enrichment and purification of the target are synchronously performed by modifying the deoxyribonucleic acid aptamer and the solid-phase microextraction layer of the silicon dioxide fiber of the optical waveguide sensor. The method does not need any enzyme-based signal amplification reaction, and realizes the rapid detection with ultrahigh sensitivity and ultrahigh specificity on various small molecular targets. The detection limit is very low, and the method has excellent universality. The method can be used for directly detecting the targets in the complex samples, only a liquid sample needs to be diluted, sample pretreatment is not needed, and the detection sensitivity meets the limit standards of each target in food and environment. The detection time is short, the regeneration speed of the sensor is high, and the sensor can be repeatedly used.)