阅读说明：本技术 一种黑胡萝卜色素提取工艺的优化方法 (Optimization method of black carrot pigment extraction process ) 是由 苏刘花 于 2020-12-18 设计创作，主要内容包括：本发明提供一种黑胡萝卜色素提取工艺的优化方法,先称取黑胡萝卜干粉,按1:15(g/mL)的比例加入纤维素酶溶液,调节溶液pH值至3.5,于75℃恒温水浴中浸提1h,而后在转速4000r/min的条件下离心40min,过滤,将滤渣浸提1次后合并滤液并定容至200mL,测定吸光值后用X-5树脂吸附,再用乙醇溶液洗脱,得到黑胡萝卜色素提取物；本申请采用纤维酶作为辅助提取溶液,反应条件温和,最大程度地保证黑萝卜色素的品质,且有机溶剂消耗少,提取效率高,环保节能,可进行规模化工业生产。(The invention provides an optimization method of a black carrot pigment extraction process, which comprises the steps of weighing dry black carrot powder, adding a cellulase solution according to the proportion of 1:15 (g/mL), adjusting the pH value of the solution to 3.5, leaching for 1h in a 75 ℃ constant-temperature water bath, centrifuging for 40min at the rotation speed of 4000r/min, filtering, leaching filter residues for 1 time, combining filtrate, fixing the volume to 200mL, measuring the light absorption value, adsorbing by using X-5 resin, and eluting by using an ethanol solution to obtain a black carrot pigment extract; the method adopts the cellulase as the auxiliary extraction solution, has mild reaction conditions, ensures the quality of the black radish pigment to the maximum extent, has less consumption of organic solvent, high extraction efficiency, environmental protection and energy conservation, and can be used for large-scale industrial production.)

1. An optimization method of a black carrot pigment extraction process is characterized by comprising the following steps:

a. firstly weighing 10g of black carrot dry powder, adding 150mL of 0.8% cellulase solution according to the proportion of 1:15 (g/mL), and adjusting the pH value of the solution to 3.5;

b. leaching the solution obtained in the step a in a constant-temperature water bath at 75 ℃ for 1h, centrifuging for 40min at the rotation speed of 4000r/min, filtering, leaching the filter residue for 1 time, combining the filtrate and fixing the volume to 200 mL;

c. precisely transferring 5mL of the leaching solution into a 10mL volumetric flask, fixing the volume to a scale mark, shaking up, adjusting the pH value to 6.5, standing for 60min, and measuring the light absorption value at the maximum absorption wavelength of 533 nm;

d. adsorbing with X-5 resin, and eluting with ethanol solution to obtain black carrot pigment extract.

2. The method for optimizing the black carrot pigment extraction process according to claim 1, wherein the concentration of the cellulase solution is 0.8%, and the liquid-to-material ratio in the step a is 1: 15.

3. The method for optimizing the black carrot pigment extraction process according to claim 1, wherein the constant-temperature water bath temperature is 75 ℃ and the leaching time is 1 h.

4. The method for optimizing the black carrot pigment extraction process according to claim 1, wherein the centrifugal condition is 4000r/min of rotation speed for 40 min.

Technical Field

The invention relates to the technical field of plant deep processing, in particular to an optimization method of a black carrot pigment extraction process.

Background

The black carrot has the same characteristics as carrot, the outer skin of the fruit is purple black, and the stem is purple and orange. The black carrot pigment extracted from black carrots belongs to anthocyanin, and the black carrots have attracted wide attention at present due to the fact that the black carrot pigment is rich in anthocyanin. The black carrot pigment has strong antioxidant activity, multiple functions of resisting aging, cardiovascular diseases, cancers, improving immunity and the like, has certain pharmacological and nutritional effects, has great development potential in the fields of cosmetics, foods and medicines, and can be used as an ideal substitute material for synthetic pigments.

The extraction of black carrot pigment mainly adopts solvent extraction method, microwave extraction method, supercritical extraction method and ultrasonic extraction method. The cellulase-assisted extraction method has the advantages of high extraction efficiency, high speed and simple treatment, is widely applied to extraction processes of natural substances such as alkaloid, flavone, polysaccharide and the like at present, but has a certain blank for research on the aspect of extraction of pigments.

Disclosure of Invention

Aiming at the problems, the invention provides an optimization method of a black carrot pigment extraction process.

The purpose of the invention is realized by adopting the following technical scheme:

an optimization method of a black carrot pigment extraction process comprises the following steps:

a. firstly weighing 10g of black carrot dry powder, adding 150mL of 0.8% cellulase solution according to the proportion of 1:15 (g/mL), and adjusting the pH value of the solution to 3.5;

b. leaching the solution obtained in the step a in a constant-temperature water bath at 75 ℃ for 1h, centrifuging for 40min at the rotation speed of 4000r/min, filtering, leaching the filter residue for 1 time, combining the filtrate and fixing the volume to 200 mL;

c. precisely transferring 5mL of the leaching solution into a 10mL volumetric flask, fixing the volume to a scale mark, shaking up, adjusting the pH value to 6.5, standing for 60min, and measuring the light absorption value at the maximum absorption wavelength of 533 nm;

d. adsorbing with X-5 resin, and eluting with ethanol solution to obtain black carrot pigment extract. Preferably, the ethanol for soaking has the concentration of 90 percent and 100 mL.

Preferably, the concentration of the cellulase solution is 0.8%, and the solution-to-feed ratio in the step a is 1: 15.

Preferably, the temperature of the constant-temperature water bath is 75 ℃, and the leaching time is 1 h.

Preferably, the centrifugal condition is 4000r/min of rotation speed and 40min of time.

The invention has the beneficial effects that:

the invention inspects the optimal extraction conditions of extraction time, feed-liquid ratio and enzyme concentration through a single-factor experiment, optimizes the extraction process through an orthogonal experiment method, furthest ensures the yield and purity of the black carrot pigment, has low organic solvent consumption, high extraction efficiency, is environment-friendly, has wide market prospect, and also provides scientific basis for extraction and comprehensive development and utilization of natural plant pigment.

Detailed Description

The invention is further described with reference to the following examples.

The embodiment of the invention relates to an optimization method of a black carrot pigment extraction process, which comprises the following steps:

a. firstly weighing 10g of black carrot dry powder, adding 150mL of 0.8% cellulase solution according to the proportion of 1:15 (g/mL), and adjusting the pH value of the solution to 3.5;

b. leaching the solution obtained in the step a in a constant-temperature water bath at 75 ℃ for 1h, centrifuging for 40min at the rotation speed of 4000r/min, filtering, leaching the filter residue for 1 time, combining the filtrate and fixing the volume to 200 mL;

c. precisely transferring 5mL of the leaching solution into a 10mL volumetric flask, fixing the volume to a scale mark, shaking up, adjusting the pH value to 6.5, standing for 60min, and measuring the light absorption value at the maximum absorption wavelength of 533 nm;

d. adsorbing with X-5 resin, and eluting with ethanol solution to obtain black carrot pigment extract. Preferably, the ethanol for soaking has the concentration of 90 percent and 100 mL.

Preferably, the concentration of the cellulase solution is 0.8%, and the solution-to-feed ratio in the step a is 1: 15.

Preferably, the temperature of the constant-temperature water bath is 75 ℃, and the leaching time is 1 h.

Preferably, the centrifugal condition is 4000r/min of rotation speed and 40min of time.

Example 1

An optimization method of a black carrot pigment extraction process comprises the following steps:

weighing 10g of 5 parts of black carrot dry powder, respectively adding 0.8% cellulase solution according to the proportion of 1:5, 1:10, 1:15, 1:20 and 1:25 (g/mL), uniformly adjusting the pH value of the solution to 3.5, leaching the 5 parts of solution in a 75 ℃ constant temperature water bath for 1h, then centrifuging for 40min at the rotation speed of 4000r/min, filtering, leaching filter residues for 1 time, combining filtrates, and fixing the volume to 200 mL; precisely transferring 5mL of the leaching solution into a 10mL volumetric flask, fixing the volume to a scale mark, shaking up, adjusting the pH value to 6.5, standing for 60min, and measuring the light absorption value at the maximum absorption wavelength of 533 nm; adsorbing with X-5 resin, and eluting with ethanol solution to obtain black carrot pigment extract.

Example 2

An optimization method of a black carrot pigment extraction process comprises the following steps:

weighing 10g of 5 parts of black carrot dry powder, adding 0.8% cellulase solution according to a ratio of 1:15 (g/mL), adjusting the pH value of the solution to 3.5, leaching the 5 parts of solution in 45, 55, 65, 75 and 85 ℃ constant-temperature water bath for 1h, centrifuging for 40min at the rotation speed of 4000r/min, filtering, leaching the filter residue for 1 time, combining the filtrates, and fixing the volume to 200 mL; precisely transferring 5mL of the leaching solution into a 10mL volumetric flask, fixing the volume to a scale mark, shaking up, adjusting the pH value to 6.5, standing for 60min, and measuring the light absorption value at the maximum absorption wavelength of 533 nm; adsorbing with X-5 resin, and eluting with ethanol solution to obtain black carrot pigment extract.

Example 3

An optimization method of a black carrot pigment extraction process comprises the following steps:

weighing 10g of 5 parts of black carrot dry powder, respectively adding 0.2%, 0.4%, 0.6%, 0.8% and 1.0% cellulase solution according to a ratio of 1:15 (g/mL), adjusting the pH value of the solution to 3.5, leaching the 5 parts of solution in a 75 ℃ constant temperature water bath for 1h, then centrifuging for 40min at a rotation speed of 4000r/min, filtering, leaching filter residues for 1 time, combining filtrates, and fixing the volume to 200 mL; precisely transferring 5mL of the leaching solution into a 10mL volumetric flask, fixing the volume to a scale mark, shaking up, adjusting the pH value to 6.5, standing for 60min, and measuring the light absorption value at the maximum absorption wavelength of 533 nm; adsorbing with X-5 resin, and eluting with ethanol solution to obtain black carrot pigment extract.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.