阅读说明：本技术 一种可分离层析柱 (Separable chromatographic column ) 是由 彭贵军 于 2020-11-17 设计创作，主要内容包括：本发明涉及一种可分离层析柱,属于化学实验仪器领域；所述的可分离层析柱包括柱体和漏斗部分,所述的柱体和漏斗部分通过柱体下端的法兰面Ⅰ、漏斗部分上端的法兰面Ⅱ、活套法兰连接,所述柱体和漏斗部分均由医疗级的聚乙烯制成,所述的柱体的顶部设有供硅胶软管通过的通孔,所述柱体的外表面设置防滑的塑料外套,所述漏斗部分下端设置有抽真空管,抽真空接口设置在抽真空管上,滴液管设置在抽真空管内,本发明提供了一种可分离层析柱,能够造成柱体内液体飞溅,不会造成填料飞溅在柱体的四壁上；能够拆卸方便,部分材料可单独替换,节省成本；能够保证将固定相抽实抽干；能够保证避免夹子在柱体上留下划痕。(The invention relates to a separable chromatographic column, belonging to the field of chemical experimental instruments; the separable chromatographic column comprises a column body and a funnel part, wherein the column body and the funnel part are connected through a flange surface I at the lower end of the column body, a flange surface II at the upper end of the funnel part and a loose flange, the column body and the funnel part are both made of medical-grade polyethylene, a through hole for a silica gel hose to pass through is formed in the top of the column body, an anti-skid plastic outer sleeve is arranged on the outer surface of the column body, a vacuum pumping pipe is arranged at the lower end of the funnel part, a vacuum pumping interface is arranged on the vacuum pumping pipe, and a dropping liquid pipe is arranged in the vacuum pumping pipe; the disassembly is convenient, and part of materials can be replaced independently, so that the cost is saved; the stationary phase can be guaranteed to be pumped and dried; can guarantee to avoid the clip to leave the mar on the cylinder.)

1. A separable chromatography column, characterized by: separable chromatographic column include cylinder and funnel part, cylinder and funnel part flange face II, the loop flange joint of flange face I, funnel part upper end through the cylinder lower extreme, cylinder and funnel part are made by medical grade's polyethylene, the top of cylinder be equipped with the through-hole that supplies the silica gel hose to pass through, the surface of cylinder sets up skid-proof plastics overcoat, funnel part lower extreme is provided with the evacuation pipe, the setting of evacuation interface is on the evacuation pipe, the drip pipe sets up in the evacuation pipe.

2. A separable chromatography column according to claim 1, wherein: and a sealing rubber gasket is arranged between the flange surface I at the lower end of the column body and the flange surface II at the upper end of the funnel part.

3. A separable chromatography column according to claim 1, wherein: the bottom of the vacuum tube is provided with a layer of replaceable vacuum film.

4. A separable chromatography column according to claim 1, wherein: the upper part of the funnel part is provided with a detachable sand core sieve plate.

Technical Field

The invention relates to a separable chromatographic column, and belongs to the field of chemical experimental instruments.

Background

At present, the separation and purification of various compounds, natural products and traditional Chinese medicines mainly adopts column chromatography, uses a chromatographic column, and is realized through a column chromatography process, wherein the column chromatography is to place a mixed compound at the top end of a glass column bed, so that an eluant (a mobile phase) consisting of a mixed solvent passes through a vertical glass column filled with an adsorptive medium material (a stationary phase) under the action of gravity to be separated to obtain each purer product, and the principle is as follows: because each component in the mixed compound has different affinities for the stationary phase and the mobile phase in the chromatographic column, the proper stationary phase and mobile phase are selected according to the property of the mixed compound, the aim of separating each component as much as possible can be achieved, the separated products are respectively collected into each collection bottle at the outlet of the column, then, the collected chromatographic solutions in each collection bottle are identified, and the same chromatographic solutions are combined and concentrated according to the identification result to obtain each pure product; common chromatographic columns can be divided into atmospheric pressure columns, medium pressure columns and high pressure columns according to different pressures, the atmospheric pressure columns are mostly used in practice, and the chromatographic columns made of different materials are usually adopted by the chromatographic columns according to needs and filled with different materials to realize separation and purification of target substances.

However, in the existing chromatographic column, the sample solution directly flows into the column body from the middle part of the top end of the chromatographic column, so that the filler in the column body is easy to splash onto the inner wall of the chromatographic column, and because the filler for chromatography is usually expensive, the filler splashed onto the inner wall is not fully utilized in chromatography, and the chromatography effect is influenced; the existing chromatographic column is generally of an undetachable integrated structure, the chromatographic column with large specification and a long column body brings much inconvenience to unloading and cleaning, the chromatographic column is difficult to clean, so that compounds to be separated are polluted, the chromatographic column with large specification is expensive, and when the chromatographic column is partially damaged, the whole chromatographic column is scrapped, so that the cost is high; the existing chromatographic column can not bear larger pressure, and the stationary phase can not be pumped to be dry in the experimental process.

Therefore, there is a need to improve the existing chromatographic column, so that the chromatographic column is structurally more complete and can meet the needs of experimenters.

Disclosure of Invention

In order to overcome the problems in the background art, the invention provides a separable chromatographic column which can cause liquid in the column body to splash and can not cause filler to splash on the four walls of the column body; the disassembly is convenient, and part of materials can be replaced independently, so that the cost is saved; the stationary phase can be guaranteed to be pumped and dried; can guarantee to avoid the clip to leave the mar on the cylinder.

In order to achieve the purpose, the invention is implemented according to the following technical scheme:

separable chromatographic column include cylinder and funnel part, cylinder and funnel part flange face II, the loop flange joint of flange face I, funnel part upper end through the cylinder lower extreme, cylinder and funnel part are made by medical grade's polyethylene, the top of cylinder be equipped with the through-hole that supplies the silica gel hose to pass through, the surface of cylinder sets up skid-proof plastics overcoat, funnel part lower extreme is provided with the evacuation pipe, the setting of evacuation interface is on the evacuation pipe, the drip pipe sets up in the evacuation pipe.

Furthermore, a sealing rubber gasket is arranged between the flange surface I at the lower end of the column body and the flange surface II at the upper end of the funnel part.

Furthermore, the bottom of the vacuum tube is a layer of replaceable vacuum film.

Furthermore, the upper part of the funnel part is provided with a detachable sand core sieve plate.

The invention has the beneficial effects that: the top of the column body is provided with a through hole for the silica gel hose to pass through, when sample liquid and eluent enter from the top of the column body, the sample liquid and the eluent are left along the four walls of the column body, so that the liquid in the column body can not splash, the filler can splash onto the four walls of the column body, the height of the filler is influenced, and the purification effect of protein can not be influenced; the column body and the funnel part are connected through a flange surface I at the lower end of the column body, a flange surface II at the upper end of the funnel part and a loose flange, a sealing rubber gasket is arranged between the flange surface I at the lower end of the column body and the flange surface II at the upper end of the funnel part, so that chromatographic columns with various specifications can be prepared, particularly chromatographic columns with longer column bodies can be prepared, and the column body and the sand core funnel part can be used alternately, therefore, when a certain part is damaged, the replacement can be directly carried out, the scrapping of the whole chromatographic column cannot be caused, and the cost is; the vacuum tube is arranged at the lower end of the funnel part, the bottom of the vacuum tube is a layer of replaceable vacuum membrane, the stationary phase can be pumped to be dry, and the vacuum membrane at the bottom of the vacuum tube can be taken away after the stationary phase is pumped to be dry, so that the chromatography effect cannot be influenced; the surface of cylinder sets up skid-proof plastics overcoat, is convenient for hold the cylinder or fix the cylinder centre gripping on the frame, can not break the chromatography column to avoid the clip to leave the mar on the cylinder.

Drawings

FIG. 1 is a schematic view of the structure of the present invention.

In the figure, 1-column body, 2-funnel part, 3-flange face I, 4-flange face II, 5-loose flange, 6-through hole, 7-plastic outer sleeve, 8-vacuum tube, 9-vacuum interface, 10-dropping tube, 11-sealing rubber gasket, 12-vacuum film and 13-sand core sieve plate.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings to facilitate understanding of the skilled person.

As shown in figure 1, the separable chromatographic column comprises a column body 1 and a funnel part 2, the column body 1 and the funnel part 1 are connected through a flange surface I3 at the lower end of the column body 1, a flange surface II 4 at the upper end of the funnel part 2 and a loop flange 5, a sealing rubber gasket 11 is arranged between the flange surface I3 at the lower end of the column body 1 and the flange surface II 4 at the upper end of the funnel part 2, a detachable sand core sieve plate 13 is arranged at the upper part of the funnel part 2, when in use, the sand core sieve plate 13 is placed in the funnel part 2, then the flange surface I3 between the column body 1 and the funnel part 2 and the sealing rubber gasket 11 are filled between the flange surfaces II 4, then the column body 1 and the funnel part 2 are connected through the loop flange 5 of the column body 1 and the funnel part 2, the separable chromatographic column with the design has reasonable structural design and can be prepared into chromatographic columns with various, particularly, the column body and the funnel part of the chromatographic column with a longer column body can be connected through the flange surface I3 and the flange surface II 4, the chromatographic column is convenient to disassemble and clean, the sealing rubber gasket 11 is arranged, the air tightness is good, the column body 1 and the funnel part 2 can be used alternately, when one part is damaged, only the damaged part needs to be damaged, the whole chromatographic column cannot be scrapped, and the cost is saved;

the column body 1 and the funnel part 2 are both made of medical-grade polyethylene, the top of the column body 1 is provided with a through hole 6 for a silica gel hose to pass through, when sample liquid and eluent enter from the top of the column body 1, the sample liquid and the eluent are left along the four walls of the column body 1, so that the liquid in the column body 1 cannot splash, the filler splashes onto the four walls of the column body 1, the height of the filler is influenced, and the purification effect of protein cannot be influenced;

the outer surface of the column body 1 is provided with the anti-skid plastic outer sleeve 7, so that the column body 1 can be held by hands or the column body 1 is clamped and fixed on a frame conveniently, the chromatographic column cannot be broken, and the clamp is prevented from leaving scratches on the column body 1;

2 lower extremes of funnel part are provided with evacuation pipe 8, and evacuation interface 9 sets up on evacuation pipe 8, and dropping liquid pipe 10 sets up in evacuation pipe 8, and evacuation pipe 8 bottom is the removable vacuum membrane 12 of one deck, and 2 lower extremes of funnel part are provided with evacuation pipe 8, and evacuation pipe 8 bottom is the removable vacuum membrane 12 of one deck, and evacuation pipe 8 can be with the stationary phase pump-out futilely to can take off vacuum membrane 12 of evacuation pipe bottom 8 after with the stationary phase pump-out futilely, can not influence the chromatography effect.

The working process of the invention is as follows: when using, hold cylinder 1 or fix cylinder 1 centre gripping on the frame, place psammitolite sieve 13 in funnel part 2, then with I3 of flange face between cylinder 1 and the funnel part 2, sealed rubber gasket 11 stacks up between II 4 of flange face, then the looper flange 5 through cylinder 1 and funnel part 2 is connected cylinder 1 and funnel part 2, after vacuum tube 8 takes out the stationary phase reality and takes out futilely, take off vacuum membrane 12 of vacuum tube bottom 8, with sample liquid, the eluent gets into from the through-hole 6 at cylinder 1 top, alright carry out the chromatography.

According to the invention, the through hole for the silica gel hose to pass through is formed in the top of the column body, so that when sample liquid and eluent enter from the top of the column body, the sample liquid and the eluent are left along the four walls of the column body, the splashing of the liquid in the column body can not be caused, the filler is splashed onto the four walls of the column body, the height of the filler is influenced, and the purification effect of protein can not be influenced; the column body and the funnel part are connected through a flange surface I at the lower end of the column body, a flange surface II at the upper end of the funnel part and a loose flange, a sealing rubber gasket is arranged between the flange surface I at the lower end of the column body and the flange surface II at the upper end of the funnel part, so that chromatographic columns with various specifications can be prepared, particularly chromatographic columns with longer column bodies can be prepared, and the column body and the sand core funnel part can be used alternately, therefore, when a certain part is damaged, the replacement can be directly carried out, the scrapping of the whole chromatographic column cannot be caused, and the cost is; the vacuum tube is arranged at the lower end of the funnel part, the bottom of the vacuum tube is a layer of replaceable vacuum membrane, the stationary phase can be pumped to be dry, and the vacuum membrane at the bottom of the vacuum tube can be taken away after the stationary phase is pumped to be dry, so that the chromatography effect cannot be influenced; the surface of cylinder sets up skid-proof plastics overcoat, is convenient for hold the cylinder or fix the cylinder centre gripping on the frame, can not break the chromatography column to avoid the clip to leave the mar on the cylinder.

Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the scope of the invention as defined by the appended claims.