阅读说明：本技术 一种具有抗肠黏膜损伤的蒲公英提取物菊苣酸 (Herba Taraxaci extract cichoric acid with intestinal mucosa injury resisting effect ) 是由 朱金水 张靖 陈伟 于 2020-04-04 设计创作，主要内容包括：本发明公开了一种具有抗肠黏膜损伤的蒲公英提取物菊苣酸,属于生物科技技术领域,一种具有抗肠黏膜损伤的蒲公英提取物菊苣酸,包括脱脂乳粉、牛奶蛋白粉、乳清蛋白粉、膳食纤维、低聚麦芽糖、蒲公英提取物、菊苣酸提取物、复合菌剂、食品添加剂等,本发明可以实现对肠粘膜进行保护,从而有效避免肠粘膜的损伤,本发明中加入了膳食纤维,膳食纤维可对肠道进行有效保护,从而降低肠粘膜损伤的可能性。本发明的蒲公英提取物和菊苣酸提取物在提取过程中不引入具有毒性或者难以祛除的有机溶剂,并且采用少溶剂多次提取的工艺,降低了生产成本。(The invention discloses a dandelion extract chicoric acid with intestinal mucosa injury resistance, belonging to the technical field of biotechnology, and the dandelion extract chicoric acid with intestinal mucosa injury resistance comprises skim milk powder, milk protein powder, whey protein powder, dietary fibers, oligomeric maltose, dandelion extract, chicoric acid extract, a complex microbial inoculum, a food additive and the like. The dandelion extract and the chicoric acid extract do not introduce toxic or difficultly-removed organic solvents in the extraction process, and the production cost is reduced by adopting a process of extracting for multiple times with less solvents.)

1. A herba Taraxaci extract cichoric acid with intestinal mucosa injury resisting effect is characterized in that: comprises 30-40 parts of skim milk powder, 10-20 parts of milk protein powder, 10-20 parts of whey protein powder, 15-25 parts of dietary fiber, 5-10 parts of oligomeric maltose, 5-10 parts of dandelion extract, 5-10 parts of chicoric acid extract, 5-10 parts of complex microbial inoculum, 4-6 parts of food additive and 3-5 parts of nutrition enhancer.

2. The cichoric acid extracted from dandelion with effect of intestinal mucosa injury resistance according to claim 1, wherein: the dietary fiber is a composition of soluble dietary fiber and insoluble dietary fiber, wherein the soluble dietary fiber is one of konjac dietary fiber, pectin dietary fiber and algin dietary fiber, and the insoluble dietary fiber is one or more of wheat bran, oatmeal, whole wheat flour, brown rice and oat whole grain.

3. The cichoric acid extracted from dandelion with effect of intestinal mucosa injury resistance according to claim 1, wherein: the composite microbial inoculum comprises bifidobacterium longum, lactobacillus paracasei and lactobacillus plantarum, wherein the mass ratio of the bifidobacterium longum to the lactobacillus paracasei to the lactobacillus plantarum is 2: 1: 1.

4. the cichoric acid extracted from dandelion with effect of intestinal mucosa injury resistance according to claim 1, wherein: the dandelion extract is a dandelion root extract obtained by extracting dandelion roots, and the preparation method of the dandelion root extract comprises the following steps:

a1, putting the dried dandelion root into a pulverizer, and pulverizing to obtain dandelion root powder;

a2, putting dandelion root powder and an extraction solvent into an extraction tank for extraction to obtain dandelion root extract;

a3, filtering the dandelion root extracting solution to obtain dandelion root filtrate;

a4, concentrating the dandelion root filtrate to obtain dandelion root concentrated solution;

a5, drying the dandelion root concentrated solution to obtain the dandelion root extract.

5. The herba Taraxaci extract cichoric acid with effect of resisting intestinal mucosa injury according to claim 4, wherein: in the A2, the extraction solvent is ethanol solution, and the extraction process is at least three times of multiple extractions.

6. The cichoric acid extracted from dandelion with effect of intestinal mucosa injury resistance according to claim 5, wherein: the process of the multiple extraction comprises the following steps: heating and refluxing the dandelion root powder by using 60% ethanol solution for 2.5-3.5h to obtain a first production solution; heating and refluxing the dandelion root powder by using 60% ethanol solution for 1.5-2h to obtain a second liquid; mixing the first produced liquid and the second produced liquid, and filtering to obtain a filtrate; ultrasonic heating and reflux-extracting herba Taraxaci root powder with 80% ethanol solution for 0.5-1 hr to obtain herba Taraxaci extractive solution.

7. The herba Taraxaci extract cichoric acid with effect of resisting intestinal mucosa injury according to claim 4, wherein: in the A3, a plate-and-frame filter is adopted for the filtering process, and the pore size is 80-120 meshes.

8. The cichoric acid extracted from dandelion with effect of intestinal mucosa injury resistance according to claim 1, wherein: the preparation method of the chicoric acid extract comprises the following steps:

b1, carrying out enzymolysis on the echinacea by using glucoglycyl to obtain a primary extracting solution;

b2, extracting the primary extracting solution twice by using an ethanol water solution to obtain a chicory acid water solution; wherein the mass concentration of the acetic acid aqueous solution is 50%, the ethanol aqueous solution is subjected to acidification treatment, the pH of the ethanol solution is regulated to be less than or equal to 3 by using a dilute acid solution, the solid-liquid ratio of the echinacea purpurea to the ethanol solution is 1:6-8, and the extraction temperature is 80-100 ℃;

b3, filtering, concentrating, extracting and washing the chicory acid aqueous solution, wherein ethyl acetate is adopted for extraction in the extraction process, and the mass ratio of the ethyl acetate to the chicory acid aqueous solution is 1-1.5: 1; in the washing process, deionized water is adopted for washing once, and the pH value of the deionized water is adjusted to be less than or equal to 3;

and B4, dehydrating and drying by using anhydrous sodium sulfate, wherein the mass ratio of the anhydrous sodium sulfate to the chicoric acid aqueous solution is 1-1.5:1, and the dehydration time is 8-12h, so as to obtain the chicoric acid extract.

9. The cichoric acid extracted from dandelion with effect of intestinal mucosa injury resistance according to claim 1, wherein: the food additive is a composition of a thickening agent, a sweetening agent, an acidity regulator, an emulsifier and edible essence.

10. The cichoric acid extracted from dandelion with effect of intestinal mucosa injury resistance according to claim 1, wherein: the nutrition enhancer is a composition of amino acids, vitamins and minerals.

Technical Field

The invention relates to the technical field of biotechnology, and particularly relates to a dandelion extract cichoric acid with intestinal mucosa injury resistance.

Background

The intestinal tract is an important digestive organ of a human body and also a largest detoxifying organ of the human body, so that the state of the intestinal tract determines the appearance and beauty of the human body. The intestine refers to the digestive tract from the stomach pylorus to the anus, and is the longest segment of the digestive tract, and is also the most important segment in function. The mammalian intestine includes the small intestine, large intestine and 3 large segments of the rectum. The large volume of digestion and the absorption of almost all the products of digestion are carried out in the small intestine, which concentrates mainly the food residues, forming feces, which are then excreted outside the body through the rectum and anus.

The loss or destruction of the intestinal mucosal barrier function can lead to repeated inflammation of the intestinal mucosa, causing Ulcerative Colitis (UC) with clinical manifestations of abdominal pain, abdominal distension, and mucous bloody stools, with weight loss and fever, epigastric discomfort, belching, anorexia, nausea, vomiting, etc. The main pathological changes of the colon cancer mainly comprise congestion, swelling, erosion and shallow ulcer of mucosa continuously distributed in the intestinal tract, mainly relate to mucosa and submucosa of large intestine, and can be developed from rectum and far-end colon to near-end colon in a reversible way to reach the whole colon. And the risk of colon cancer, the etiology and pathogenesis of UC is still unknown, but it is associated with genetic, environmental and immunological factors. Most of the drugs currently on the market for the treatment of enteritis contain high doses of steroids and immunomodulators. The action of intestinal microorganisms has proven to be of great significance, and the regulation of intestinal flora for the treatment of diseases has become a focus of research.

Disclosure of Invention

1. Technical problem to be solved

Aiming at the problems in the prior art, the invention aims to provide a dandelion extract chicoric acid with the effect of resisting intestinal mucosa injury.

2. Technical scheme

In order to solve the above problems, the present invention adopts the following technical solutions.

A herba Taraxaci extract chicoric acid with intestinal mucosa injury resisting effect comprises 30-40 parts of skimmed milk powder, 10-20 parts of milk protein powder, 10-20 parts of whey protein powder, 15-25 parts of dietary fiber, 5-10 parts of malto oligosaccharide, 5-10 parts of herba Taraxaci extract, 5-10 parts of chicoric acid extract, 5-10 parts of composite microbial inoculum, 4-6 parts of food additive and 3-5 parts of nutrition enhancer.

Furthermore, the dietary fiber is a composition of soluble dietary fiber and insoluble dietary fiber, wherein the soluble dietary fiber is one of konjac dietary fiber, pectin dietary fiber and alginate gel dietary fiber, and the insoluble dietary fiber is one or a composition of more than two of wheat bran, oatmeal, whole wheat flour, brown rice and oat whole grain.

Further, the composite microbial inoculum comprises bifidobacterium longum, lactobacillus paracasei and lactobacillus plantarum, wherein the mass ratio of the bifidobacterium longum to the lactobacillus paracasei to the lactobacillus plantarum is 2: 1: 1.

further, the dandelion extract is a dandelion root extract obtained by extracting dandelion roots, and the preparation method of the dandelion root extract comprises the following steps:

a1, putting the dried dandelion root into a pulverizer, and pulverizing to obtain dandelion root powder;

a2, putting dandelion root powder and an extraction solvent into an extraction tank for extraction to obtain dandelion root extract;

a3, filtering the dandelion root extracting solution to obtain dandelion root filtrate;

a4, concentrating the dandelion root filtrate to obtain dandelion root concentrated solution;

a5, drying the dandelion root concentrated solution to obtain the dandelion root extract.

Further, in the A2, the extraction solvent is ethanol solution, and the extraction process is at least three times of multiple extractions.

Further, the process of extracting for multiple times is as follows: heating and refluxing the dandelion root powder by using 60% ethanol solution for 2.5-3.5h to obtain a first production solution; heating and refluxing the dandelion root powder by using 60% ethanol solution for 1.5-2h to obtain a second liquid; mixing the first produced liquid and the second produced liquid, and filtering to obtain a filtrate; ultrasonic heating and reflux-extracting herba Taraxaci root powder with 80% ethanol solution for 0.5-1 hr to obtain herba Taraxaci extractive solution.

Further, in the A3, a plate-and-frame filter is adopted for the roller in the filtering process, and the pore size is 80-120 meshes.

Further, the preparation method of the chicoric acid extract comprises the following steps:

b1, carrying out enzymolysis on the echinacea by using glucoglycyl to obtain a primary extracting solution;

b2, extracting the primary extracting solution twice by using an ethanol water solution to obtain a chicory acid water solution; wherein the mass concentration of the acetic acid aqueous solution is 50%, the ethanol aqueous solution is subjected to acidification treatment, the pH of the ethanol solution is regulated to be less than or equal to 3 by using a dilute acid solution, the solid-liquid ratio of the echinacea purpurea to the ethanol solution is 1:6-8, and the extraction temperature is 80-100 ℃;

b3, filtering, concentrating, extracting and washing the chicory acid aqueous solution, wherein ethyl acetate is adopted for extraction in the extraction process, and the mass ratio of the ethyl acetate to the chicory acid aqueous solution is 1-1.5: 1; in the washing process, deionized water is adopted for washing once, and the pH value of the deionized water is adjusted to be less than or equal to 3;

and B4, dehydrating and drying by using anhydrous sodium sulfate, wherein the mass ratio of the anhydrous sodium sulfate to the chicoric acid aqueous solution is 1-1.5:1, and the dehydration time is 8-12h, so as to obtain the chicoric acid extract.

Further, the food additive is a composition of a thickening agent, a sweetening agent, an acidity regulator, an emulsifier and edible essence.

Furthermore, the nutrition enhancer is a composition of amino acid, vitamin and mineral.

3. Advantageous effects

Compared with the prior art, the invention has the advantages that:

(1) the compound microbial inoculum consisting of the bifidobacterium longum, the lactobacillus paracasei and the lactobacillus plantarum is added, the bifidobacterium longum, the lactobacillus paracasei and the lactobacillus plantarum all play an effective protection role on intestinal mucosa, the bifidobacterium longum plays a leading role in the protection of the intestinal mucosa, the lactobacillus paracasei and the lactobacillus plantarum are synergized, and the protection effect on the intestinal mucosa is improved.

(2) In the invention, a chicoric acid extract is added, and the chicoric acid is one of the most important immune active ingredients in chicory and echinacea purpurea. Recent pharmacological studies show that chicoric acid has the effects of enhancing the immune function and resisting inflammation, so that intestinal mucosa injury can be effectively prevented, hyaluronidase can be inhibited, and collagen can be protected from free radicals which can cause degradation. The chicoric acid extraction method has the characteristics of high purity, no chemical residue, safety and no toxicity, and has simple and efficient preparation process, low price of required production equipment and reagents and low production cost.

(3) The dandelion extract is also added in the invention, the dandelion extract is the root extract of dandelion, and the root of dandelion contains dandelion alcohol, psi-glucosyl sterol, dandelion sterol, beta-balsamic alcohol, stigmasterol, beta-sitosterol, choline, organic acid, fructose, sucrose, glucose, glucoside, resin, rubber and the like. The roots of dandelion are used for treating liver and gallbladder obstruction, improving liver function, promoting bile secretion and as diuretic. In addition, the dandelion extract is extracted without introducing toxic or difficultly-removed organic solvents, and the production cost is reduced by adopting a process of extracting for multiple times with less solvents.

(4) The dietary fiber is added in the invention, and is a composition of soluble dietary fiber and insoluble dietary fiber. The soluble fiber is interwoven with carbohydrates such as starch and the like in the gastrointestinal tract, delays the absorption of the carbohydrates, can play a role in reducing postprandial blood sugar and also has an effective bowel relaxing function; the insoluble fiber has the effects of promoting gastrointestinal peristalsis, accelerating food to pass through the gastrointestinal tract and reducing absorption on a human body, and can absorb water in the large intestine to soften stool so as to prevent and treat constipation; the dietary fiber can effectively protect the intestinal tract, thereby reducing the possibility of intestinal mucosa injury.

Drawings

FIG. 1 is a flow chart of the method for preparing the dandelion root extract of the present invention;

FIG. 2 is a flow chart of the process for the preparation of chicoric acid extract according to the present invention.

Detailed Description

The drawings in the embodiments of the invention will be combined; the technical scheme in the embodiment of the invention is clearly and completely described; obviously; the described embodiments are only some of the embodiments of the invention; but not all embodiments, are based on the embodiments of the invention; all other embodiments obtained by a person skilled in the art without making any inventive step; all fall within the scope of protection of the present invention.

Example 1:

a herba Taraxaci extract chicoric acid with intestinal mucosa injury resisting effect comprises 30-40 parts of skimmed milk powder, 10-20 parts of milk protein powder, 10-20 parts of whey protein powder, 15-25 parts of dietary fiber, 5-10 parts of malto oligosaccharide, 5-10 parts of herba Taraxaci extract, 5-10 parts of chicoric acid extract, 5-10 parts of composite microbial inoculum, 4-6 parts of food additive and 3-5 parts of nutrition enhancer.

The dietary fiber is a composition of soluble dietary fiber and insoluble dietary fiber, wherein the soluble dietary fiber is one of konjac dietary fiber, pectin dietary fiber and algin dietary fiber, and the insoluble dietary fiber is one or more of wheat bran, oatmeal, whole wheat flour, brown rice and oat whole grain.

The composite microbial inoculum comprises bifidobacterium longum, lactobacillus paracasei and lactobacillus plantarum, wherein the mass ratio of the bifidobacterium longum to the lactobacillus paracasei to the lactobacillus plantarum is 2: 1: 1.

the herba Taraxaci extract is obtained by extracting radix Taraxaci.

Referring to fig. 1, the preparation method of dandelion root extract comprises the following steps:

a1, putting the dried dandelion root into a pulverizer, and pulverizing to obtain dandelion root powder;

a2, putting dandelion root powder and an extraction solvent into an extraction tank for extraction to obtain dandelion root extract;

a3, filtering the dandelion root extracting solution to obtain dandelion root filtrate;

a4, concentrating the dandelion root filtrate to obtain dandelion root concentrated solution;

a5, drying the dandelion root concentrated solution to obtain the dandelion root extract.

In A2, the extraction solvent is ethanol solution, and the extraction process is repeated for at least three times.

The process of multiple extraction is as follows: heating and refluxing the dandelion root powder by using 60% ethanol solution for 2.5-3.5h to obtain a first production solution; heating and refluxing the dandelion root powder by using 60% ethanol solution for 1.5-2h to obtain a second liquid; mixing the first produced liquid and the second produced liquid, and filtering to obtain a filtrate; ultrasonic heating and reflux-extracting herba Taraxaci root powder with 80% ethanol solution for 0.5-1 hr to obtain herba Taraxaci extractive solution.

In A3, a plate-and-frame filter is adopted for the filtering process, and the pore size is 80-120 meshes.

Referring to fig. 2, the preparation method of chicoric acid extract comprises:

b1, carrying out enzymolysis on the echinacea by using glucoglycyl to obtain a primary extracting solution;

b2, extracting the primary extracting solution twice by using an ethanol water solution to obtain a chicory acid water solution; wherein the mass concentration of the acetic acid aqueous solution is 50%, the ethanol aqueous solution is subjected to acidification treatment, the pH of the ethanol solution is regulated to be less than or equal to 3 by using a dilute acid solution, the solid-liquid ratio of the echinacea purpurea to the ethanol solution is 1:6-8, and the extraction temperature is 80-100 ℃;

b3, filtering, concentrating, extracting and washing the chicory acid aqueous solution, wherein ethyl acetate is adopted for extraction in the extraction process, and the mass ratio of the ethyl acetate to the chicory acid aqueous solution is 1-1.5: 1; in the washing process, deionized water is adopted for washing once, and the pH value of the deionized water is adjusted to be less than or equal to 3;

and B4, dehydrating and drying by using anhydrous sodium sulfate, wherein the mass ratio of the anhydrous sodium sulfate to the chicoric acid aqueous solution is 1-1.5:1, and the dehydration time is 8-12h, so as to obtain the chicoric acid extract.

The food additive is a composition of thickening agent, sweetener, acidity regulator, emulsifier and edible essence.

The nutrition enhancer is a composition of amino acids, vitamins and minerals.

The above; but are merely preferred embodiments of the invention; the scope of the invention is not limited thereto; any person skilled in the art is within the technical scope of the present disclosure; the technical scheme and the improved concept of the invention are equally replaced or changed; are intended to be covered by the scope of the present invention.