阅读说明：本技术 一种用于显微系统的可调多波长数据采集系统 (Adjustable multi-wavelength data acquisition system for microscope system ) 是由 程鸿 张齐杨 王丽 于 2021-07-02 设计创作，主要内容包括：本发明提供一种用于显微系统的可调多波长数据采集系统,包括下层圆环底座、上层圆环支撑架、固定在下层圆环底座和上层圆环支撑架上的自动伸缩支撑杆、固定在上层圆环支撑架上的杆状传动装置、安装在杆状传动装置上的滤光片、固定在下层圆环底座上的信号接收装置以及与信号接收装置信号连接的控制装置。本发明采用双层圆环架空结构,可以在正置/倒置显微系统下使用,具有轻便、小巧、适用范围广的特点；本发明能够在不进行机械移动的前提下,快速精准地在显微系统下采集不同波长下的多幅强度图像,为后续利用采集到的数据进行多波长条件下的相位恢复提供了基础。(The invention provides an adjustable multi-wavelength data acquisition system for a microscope system, which comprises a lower-layer circular ring base, an upper-layer circular ring support frame, an automatic telescopic support rod, a rod-shaped transmission device, an optical filter, a signal receiving device and a control device, wherein the automatic telescopic support rod is fixed on the lower-layer circular ring base and the upper-layer circular ring support frame, the rod-shaped transmission device is fixed on the upper-layer circular ring support frame, the optical filter is installed on the rod-shaped transmission device, the signal receiving device is fixed on the lower-layer circular ring base, and the control device is in signal connection with the signal receiving device. The invention adopts a double-layer circular ring overhead structure, can be used under an upright/inverted microscope system, and has the characteristics of light weight, small size and wide application range; the invention can rapidly and accurately acquire a plurality of intensity images under different wavelengths under a microscope system on the premise of not mechanically moving, and provides a basis for subsequently utilizing acquired data to carry out phase recovery under the condition of multiple wavelengths.)

1. An adjustable multi-wavelength data acquisition system for a microscopy system, characterized by: the system comprises a lower-layer circular ring base, an upper-layer circular ring support frame, an automatic telescopic support rod, a rod-shaped transmission device, an optical filter, a signal receiving device and a control device;

the upper-layer circular ring support frame and the lower-layer circular ring base are arranged in parallel and are positioned right above the lower-layer circular ring base; the automatic telescopic support rods are at least two, one end of each automatic telescopic support rod is fixed on the lower-layer circular ring base, and the other end of each automatic telescopic support rod is fixed on the upper-layer circular ring support frame;

one end of the rod-shaped transmission device is fixed on the upper-layer circular ring support frame, and the other end of the rod-shaped transmission device extends to the center of the upper-layer circular ring support frame; the optical filter is arranged in parallel with the upper-layer circular ring support frame, a circular rotary multiband optical filter is adopted, and the position of the circle center of the circular rotary multiband optical filter is fixed at one end of the rod-shaped transmission device extending to the center of the upper-layer circular ring support frame;

the signal receiving device is fixed on the lower-layer circular ring base and used for controlling the stretching of the automatic stretching supporting rod and the rotation of the optical filter according to an instruction sent by the control device;

and the control device is used for sending an instruction for controlling the stretching of the automatic telescopic supporting rod and an instruction for controlling the rotation of the optical filter to the signal receiving device.

2. The tunable multi-wavelength data acquisition system for microscopy system according to claim 1, wherein: the number of the automatic telescopic supporting rods is four, and the central angle between two adjacent automatic telescopic supporting rods is 90 degrees.

3. The tunable multi-wavelength data acquisition system for microscopy system according to claim 1, wherein: the rod-shaped transmission device is formed by integrally connecting a downward bent part and a linear part, one end of the downward bent part is fixed on the upper-layer circular support frame, the other end of the downward bent part is connected with one end of the linear part, and the other end of the linear part extends to the center of the upper-layer circular support frame.

4. The tunable multi-wavelength data acquisition system for microscopy system according to claim 1, wherein: the outer diameters of the lower-layer circular ring base and the upper-layer circular ring support frame are both 10cm, the inner diameters of the lower-layer circular ring base and the upper-layer circular ring support frame are both 9cm, and the thicknesses of the lower-layer circular ring base and the upper-layer circular ring support frame are both 0.5 cm; the adjusting range of the automatic telescopic supporting rod is 2 cm-4 cm; the signal receiving device is in a cuboid shape, and the length, the width and the height of the signal receiving device are 0.5cm x 1.5 cm.

5. The tunable multi-wavelength data acquisition system for microscopy system according to claim 1, wherein: the inner parts of the lower-layer circular ring base, the upper-layer circular ring support frame, the automatic telescopic supporting rod, the rod-shaped transmission device and the signal receiving device are all hollow structures and communicated at the connecting part for placing a lead;

the interior of the automatic telescopic supporting rod and one end of the rod-shaped transmission device extending to the center of the upper-layer circular ring supporting frame are both provided with a micro motor;

the button cell is installed in the base of the signal receiver as a power supply, the input end of the signal receiver is connected with the output end of the control device, and the output end of the signal receiver is connected with the positive electrode and the negative electrode of each micro motor through a lead.

6. The tunable multi-wavelength data acquisition system for microscopy system according to claim 1, wherein: the control device adopts a computer.

Technical Field

The invention relates to the technical field of biological microscopic imaging, in particular to an adjustable multi-wavelength data acquisition system for a microscopic system.

Background

Microscopic optical imaging is a technique in which visible light passes through a sample and then passes through one or more lenses to magnify a microscopic image of the sample. The image can be directly observed through an ocular lens, or can be recorded by a light-sensitive plate and a digital image detector (CCD), and can be displayed and processed on a computer.

In microscopic imaging, most samples are colorless and transparent biological samples, and when light waves are transmitted, intensity information and phase information of the samples are contained in the transmitted light. Studies have shown that about three quarters of the information is stored in the phase, while only one quarter of the information is stored in the amplitude. Therefore, phase estimation (recovery) from the intensity distribution of the sample, i.e., the phase recovery problem, has attracted a wide attention.

The amplitude of the object light wave can be directly acquired by the camera, but the phase cannot be directly detected. The most classical phase measurement method is interferometry, however this method suffers from the following disadvantages:

(1) the light waves enter the sensor area along different independent paths, and the measurement result is seriously damaged (caused by environmental interference) under the influence of vibration;

(2) the temporal coherence requirements of the light source are high, requiring relatively complex interference devices, etc.

Another very important class of non-interferometric phase measurement techniques is known as phase recovery techniques. The phase recovery method based on the intensity transmission equation is a typical method, and the method can recover phase information by solving the equation only by measuring the light intensity distribution of the light wave to be measured at different transmission distances. The equation is in the form:

wherein the light wave propagates along the z-direction, λ represents the wavelength of the light, I andrespectively represents z₀The intensity and phase of the location. In this equation, the partial derivative of the intensity in the z direction is difficult to calculate, and it is usually obtained by acquiring a plurality of intensity images to approximate. For example, z can be used₀+ Δ z and z₀The intensity information of the position is obtained by the following differential calculation formula:

however, the non-interference phase recovery technology based on the intensity transmission equation is only suitable for phase solution in short-distance transmission under the condition of single wavelength, and a phase recovery algorithm under the condition of multi-wavelength overcomes the limitation of short-distance transmission and single wavelength at present. The method comprises the steps of firstly, respectively obtaining phase distribution under a plurality of different wavelengths by utilizing a TIE algorithm, then, considering the relevant constraint of the phase between two wavelengths, reconstructing the phase distribution of a recording surface by combining the idea of synthesizing the wavelengths, and finally, reversely transmitting the phase distribution to an object surface by utilizing an angular spectrum method to obtain the recovered object surface phase.

After the phase distribution at a single wavelength is obtained, the phase distribution at the synthesized wavelength can be obtained by a multi-wavelength method. The composite phase distribution at dual wavelength is:

wherein h represents an optical path difference when light passes through an object to be measured,expressed as equivalent wavelengths at two wavelengths, the value of which is greater than either wavelength.

The phase distribution of the three-wavelength synthesis is:

wherein i, j, k are three different wavelengths respectively,

in the conventional experiment, if a phase recovery algorithm under a multi-wavelength condition is required to solve the phase, corresponding intensity images under different wavelengths need to be acquired respectively, and mechanical movement can be caused in the process of replacing the optical filter in order to acquire the intensity images under different wavelengths, so that a final phase recovery result can be influenced.

Disclosure of Invention

The invention aims to provide an adjustable multi-wavelength data acquisition system for a microscope system, which can acquire a plurality of intensity images under different wavelengths on the premise of not carrying out mechanical movement.

The technical scheme of the invention is as follows:

an adjustable multi-wavelength data acquisition system for a microscope system comprises a lower-layer circular ring base, an upper-layer circular ring support frame, an automatic telescopic supporting rod, a rod-shaped transmission device, an optical filter, a signal receiving device and a control device;

the upper-layer circular ring support frame and the lower-layer circular ring base are arranged in parallel and are positioned right above the lower-layer circular ring base; the automatic telescopic support rods are at least two, one end of each automatic telescopic support rod is fixed on the lower-layer circular ring base, and the other end of each automatic telescopic support rod is fixed on the upper-layer circular ring support frame;

one end of the rod-shaped transmission device is fixed on the upper-layer circular ring support frame, and the other end of the rod-shaped transmission device extends to the center of the upper-layer circular ring support frame; the optical filter is arranged in parallel with the upper-layer circular ring support frame, a circular rotary multiband optical filter is adopted, and the position of the circle center of the circular rotary multiband optical filter is fixed at one end of the rod-shaped transmission device extending to the center of the upper-layer circular ring support frame;

the signal receiving device is fixed on the lower-layer circular ring base and used for controlling the stretching of the automatic stretching supporting rod and the rotation of the optical filter according to an instruction sent by the control device;

and the control device is used for sending an instruction for controlling the stretching of the automatic telescopic supporting rod and an instruction for controlling the rotation of the optical filter to the signal receiving device.

The adjustable multi-wavelength data acquisition system for the microscope system is characterized in that the number of the automatic telescopic supporting rods is four, and the central angle between two adjacent automatic telescopic supporting rods is 90 degrees.

The adjustable multi-wavelength data acquisition system for the microscope system is characterized in that the rod-shaped transmission device is formed by integrally connecting a downward bending part and a linear part, one end of the downward bending part is fixed on the upper-layer circular ring support frame, the other end of the downward bending part is connected with one end of the linear part, and the other end of the linear part extends to the center of the upper-layer circular ring support frame.

In the adjustable multi-wavelength data acquisition system for the microscope system, the outer diameters of the lower-layer ring base and the upper-layer ring support frame are both 10cm, the inner diameters thereof are both 9cm, and the thicknesses thereof are both 0.5 cm; the adjusting range of the automatic telescopic supporting rod is 2 cm-4 cm; the signal receiving device is in a cuboid shape, and the length, the width and the height of the signal receiving device are 0.5cm x 1.5 cm.

The adjustable multi-wavelength data acquisition system for the microscope system is characterized in that the lower-layer ring base, the upper-layer ring support frame, the automatic telescopic support rod, the rod-shaped transmission device and the signal receiving device are all hollow structures and communicated at the joints for placing leads;

the interior of the automatic telescopic supporting rod and one end of the rod-shaped transmission device extending to the center of the upper-layer circular ring supporting frame are both provided with a micro motor;

the button cell is installed in the base of the signal receiver as a power supply, the input end of the signal receiver is connected with the output end of the control device, and the output end of the signal receiver is connected with the positive electrode and the negative electrode of each micro motor through a lead.

The adjustable multi-wavelength data acquisition system for the microscope system is characterized in that the control device adopts a computer.

According to the technical scheme, the double-layer circular ring overhead structure is adopted, can be used under an upright/inverted microscope system, and has the characteristics of light weight, small size and wide application range; the invention can rapidly and accurately acquire a plurality of intensity images under different wavelengths under a microscope system on the premise of not mechanically moving, and provides a basis for subsequently utilizing acquired data to carry out phase recovery under the condition of multiple wavelengths.

Drawings

Fig. 1 is a schematic structural view of the present invention.

Detailed Description

The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

As shown in fig. 1, an adjustable multi-wavelength data acquisition system for a microscope system includes a lower ring base 1, an upper ring support frame 2, four automatic telescopic support rods 31, 32, 33, and 34, a rod-shaped transmission device 4, an optical filter 5, a signal receiving device 6, and a control device 7.

The upper layer circular ring support frame 2 and the lower layer circular ring base 1 are arranged in parallel and are positioned right above the lower layer circular ring base 1.

The four automatic telescopic supporting rods 31, 32, 33 and 34 have the same specification and the telescopic lengths are completely consistent, one end of each automatic telescopic supporting rod is fixed on the lower layer circular ring base 1, and the other end of each automatic telescopic supporting rod is fixed on the upper layer circular ring supporting frame 2. The central angle between two adjacent automatic telescopic supporting rods is 90 degrees.

The rod-shaped transmission device 4 is formed by integrally connecting a downward bending part and a linear part, one end of the downward bending part is fixed on the upper layer circular support frame 2, the other end of the downward bending part is connected with one end of the linear part, and the other end of the linear part extends to the center of the upper layer circular support frame 2.

The optical filter 5 is arranged in parallel with the upper-layer circular ring support frame 2, a circular rotary multiband optical filter is adopted, the circle center position of the circular rotary multiband optical filter is fixed at one end of the rod-shaped transmission device 4 extending to the center of the upper-layer circular ring support frame 2, and the rotation of the optical filter 5 is controlled through the rod-shaped transmission device 4. Wb1, wb2 and wb3 in fig. 1 represent three different wave bands, and filters can be freely replaced as required in an actual experiment process to acquire light waves of different wave bands and add or reduce the number of the wave bands.

The signal receiving device 6 is rectangular, fixed on the lower ring base 1, and used for controlling the expansion and contraction of the automatic expansion and contraction support rods 31, 32, 33 and 34 and the rotation of the optical filter 5 according to the instruction sent by the control device 7.

The control device 7 employs a computer for issuing instructions for controlling the expansion and contraction of the automatic expansion and contraction support rods 31, 32, 33, and 34 and instructions for controlling the rotation of the optical filter 5 to the signal receiving device 6.

The lower-layer circular ring base 1, the upper-layer circular ring support frame 2, the automatic telescopic support rods 31, 32, 33 and 34, the rod-shaped transmission device 4 and the signal receiving device 6 are all hollow structures and communicated at the joints for placing wires. The interior of the automatic telescopic supporting rods 31, 32, 33 and 34 and one end of the rod-shaped transmission device 4 extending to the center of the upper-layer circular ring supporting frame 2 are provided with micro motors.

The signal receiver is arranged in the signal receiving device 6, a button battery is installed in a base of the signal receiver to serve as a power supply, the input end of the signal receiver is connected with the output end of the control device 7 to receive instruction signals sent by the control device 7, and the output end of the signal receiver is connected with the positive electrode and the negative electrode of the five micro motors through ten leads to control the four automatic telescopic supporting rods 31, 32, 33 and 34 and the rod-shaped transmission device 4.

The control of the automatically telescoping support rods 31, 32, 33 and 34 is described in detail below, taking the automatically telescoping support rod 31 as an example:

when a stretching instruction signal from the control device 7 is received, the signal receiver is positively connected with the micro motor inside the automatic telescopic supporting rod 31, the automatic telescopic supporting rod 31 automatically stretches, and when a stopping instruction signal is received, the signal receiver cuts off a loop, and the automatic telescopic supporting rod 31 stops stretching; if the control device 7 still does not send out a stop command signal when the maximum adjustable range is reached, the signal receiver will automatically cut off the loop. If the control device 7 sends a contraction command signal, the signal receiver will reverse the electrode, and the micro motor will reverse to complete the operation of automatically contracting the telescopic supporting rod 31. In actual operation, as long as the command signal from the control device 7 is received, the micro motors inside the automatic telescopic supporting rods 31, 32, 33 and 34 are simultaneously operated, and the height adjustment is simultaneously completed.

The control of the rod gear 4 is explained in detail below:

it has been explained above that the lower ring base 1, the upper ring support frame 2, the automatic telescopic support rods 31, 32, 33 and 34, the rod-shaped transmission device 4 and the signal receiving device 6 are all hollow structures and are communicated at the joints for placing wires, wherein two wires are used for controlling the rod-shaped transmission device 4 to complete the rotation of the optical filter 5, the two wires pass through the lower ring base 1, the automatic telescopic support rods 31 and 34 and the upper ring support frame 2 and are directly connected to the positive electrode and the negative electrode of the micro motor at the right end of the rod-shaped transmission device 4, and the optical filter 5 is directly installed at the output end of the micro motor. When receiving a command signal from the control device 7 for controlling the filter 5 to rotate clockwise, the signal receiver controls the rotation direction of the micro motor to drive the filter 5 to rotate forward, otherwise, the electrode of the micro motor is reversed to realize the reverse rotation of the filter 5.

For convenience of description, a double-layer ring overhead structure composed of the lower ring base 1, the upper ring support frame 2, the four automatic telescopic support rods 31, 32, 33 and 34, the rod-shaped transmission device 4, the optical filter 5 and the signal receiving device 6 is referred to as a double-layer ring device.

Due to the limitation of experimental conditions, the outer diameter of the whole double-layer circular ring device is set to be 10cm, wherein the outer diameters of the lower-layer circular ring base 1 and the upper-layer circular ring support frame 2 are both 10cm, the inner diameters are both 9cm, and the thicknesses are both 0.5 cm; the initial height of the automatic telescopic support rods 31, 32, 33 and 34 is 3cm, the adjustable range is 2 cm-4 cm, and the adjusting speed is 1 mm/s; the initial height of the whole double-layer circular device is 4cm, and the height of the whole double-layer circular device can be adjusted between 3cm and 5cm so as to adapt to most experimental conditions and ensure the general applicability of the invention. The size of the signal receiving device 6 is 0.5cm x 0.5cm on the bottom surface and 1.5cm in height, so that the signal receiving device 6 is not influenced when the automatic telescopic supporting rods 31, 32, 33 and 34 are adjusted to the minimum height.

The rod-shaped transmission device 4 is positioned at the middle point of the two automatic telescopic supporting rods 31 and 34, and considering that the thickness of the optical filter 5 can influence the height of the whole double-layer circular ring device, the rod-shaped transmission device 4 is not designed into a structure which extends straightly towards the right, but extends downwards 0.5cm from the left end and then extends towards the right for 3.5cm to the circle center, and therefore the design avoids the thickness of the optical filter 5 from influencing the height of the whole double-layer circular ring device.

Because the optical filter 5 is arranged at the right end of the rod-shaped transmission device 4, the left side of the optical filter 5 covers the rod-shaped transmission device 4, light beams pass through the right side of the optical filter 5, the light beams can be used from top to bottom or from bottom to top, and the diameter of the optical filter 5 can be used between 1cm and 7cm, so that the universality of the invention is greatly improved. The rotation of the filter 5 is controlled by the control device 7, the rotation rate is controlled to be 60s/r, and the filter can rotate in the forward direction and the reverse direction and can be stopped.

The working principle of the invention is as follows:

the control device 7 is paired with the double-layer ring device, the whole double-layer ring device is placed under the light path of the positive (or inverted) microscope system, the light path of the double-layer ring device passes through the right side of the optical filter 5, then the automatic telescopic support rods 31, 32, 33 and 34 are adjusted to proper heights to adapt to experimental conditions, the optical filter 5 is controlled to rotate to obtain light with different wavelengths, and a camera is used for collecting focusing and defocusing intensity images under different wavelengths. After the acquisition is completed, a phase recovery algorithm under the condition of multi-wavelength is adopted to solve the phase.

The above-mentioned embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements made to the technical solution of the present invention by those skilled in the art without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.