阅读说明：本技术 一种用于细胞筛选显微镜载物台的自动进给装置 (Automatic feeding device for cell screening microscope stage ) 是由 秦玲 胡志刚 冯金彪 邓会阳 付东辽 于 2020-08-25 设计创作，主要内容包括：一种用于细胞筛选显微镜载物台的自动进给装置,涉及细胞筛选自动化设备领域,本发明通过在载物平台(6)上设置横向进给机构和纵向进给机构,在现有人工对焦显微镜载物台安装空间狭小、移动范围有限的情况下,实现了对载物台的自动进给,为实现对现有人工对焦细胞筛选显微镜的自动化改造提供了有力支撑等,本发明具有设计精巧,体积小、精度高等特点,适合大范围的推广和应用。(The invention relates to an automatic feeding device for an objective table of a cell screening microscope, which relates to the field of cell screening automation equipment, and realizes automatic feeding of the objective table under the conditions of narrow installation space and limited moving range of the existing manual focusing microscope objective table by arranging a transverse feeding mechanism and a longitudinal feeding mechanism on an objective table (6), thereby providing powerful support and the like for realizing automatic modification of the existing manual focusing cell screening microscope.)

1. An automatic feeding device for a cell screening microscope stage comprises a stage (6), a transverse feeding mechanism and a longitudinal feeding mechanism, and is characterized in that: a guide rail B (26) in the longitudinal feeding mechanism is arranged on the side edge of the object platform (6), a rack A (23) is arranged below the guide rail B (26), a slide block B (27) is arranged in the guide rail B (26), a connecting frame (28) is arranged on the slide block B (27), a supporting plate (29) is arranged on the lower portion of the connecting frame (28), a stepping motor C (16) and a stepping motor B (10) are respectively arranged on the upper portion and the lower portion of the supporting plate (29), a belt wheel B (13) and a belt wheel A (11) are respectively arranged on main shafts of the stepping motor C (16) and the stepping motor B (10), the belt wheel B (13) and the belt wheel A (11) are respectively connected with a belt wheel D (21) arranged on the lower portion of the outer edge surface of a hollow gear shaft (22) and a belt wheel C (20) arranged on the outer edge surface of the main shaft (19) through a transmission belt, the hollow gear shaft (22) is sleeved on the outer edge surface of, the tooth of a cogwheel on hollow gear axle (22) and rack A (23) meshing, the both ends of main shaft (19) are passed through flange (24) and bearing frame (32) respectively and are set up on backup pad (29), are equipped with gear (25) at the upper end of main shaft (19), gear (25) and the rack B (33) meshing among the transverse feed mechanism, rack B (33) set up below slider A (17), slider A (17) set up in guide rail A (18), guide rail A (18) set up the higher authority in link (28), be equipped with on slider A (17) bracket (5) form the automatic feeding device who is used for cell screening microscope objective table.

2. The automatic feeding device for a cell screening microscope stage according to claim 1, characterized in that: the stepping motor C (16) is fixed on the upper part of the connecting frame (28) through a motor mounting plate A (30), and the stepping motor C (16) is connected with the control system through a circuit.

3. The automatic feeding device for a cell screening microscope stage according to claim 1, characterized in that: the stepping motor B (10) is fixed on the lower part of the connecting frame (28) through a motor mounting plate B (31), and the stepping motor B (10) is connected with the control system through a circuit.

4. The automatic feeding device for a cell screening microscope stage according to claim 1, characterized in that: the rear end part of the object carrying platform (6) is fixed on a bracket of the microscope.

5. The automatic feeding device for a cell screening microscope stage according to claim 1, characterized in that: and a bearing (34) is arranged between the outer edge surface of the lower end head of the main shaft (19) and the bearing seat (32).

6. The automatic feeding device for a cell screening microscope stage according to claim 1, characterized in that: and a 96-hole plate (2) is arranged on the bracket (5).

7. The automatic feeding device for a cell screening microscope stage according to claim 1, characterized in that: the transmission belt is any one of a synchronous belt, a multi-wedge belt or a triangular belt.

8. The automatic feeding device for a cell screening microscope stage according to claim 1, characterized in that: the belt wheel C (20) is connected with the main shaft (19) through a flat key, or a jackscrew hole is formed in the outer edge surface of the belt wheel C (20), and a jackscrew is arranged in the jackscrew hole.

9. The automatic feeding device for a cell screening microscope stage according to claim 1, characterized in that: and the outer edge surface of the belt wheel D (21) is provided with a jackscrew hole, and a jackscrew is arranged in the jackscrew hole.

10. The automatic feeding device for a cell screening microscope stage according to claim 1, characterized in that: and a bearing is arranged between the inner edge surface of the inner hole of the hollow gear shaft (22) and the outer edge surface of the flange (24).

Technical Field

The invention relates to the field of cell screening automation equipment, in particular to an automatic feeding device for a cell screening microscope objective table.

Background

It is known that for the culture of biological cells, 96-well plates are used as culture media, while cell screening is performed by means of a microscope, and most of the current laboratories are still manually focused microscopes. However, the 96-well plate has small cell population, difficult visual discrimination, low manual screening detection rate and easy fatigue. Therefore, the work of screening for such cells is urgently needed to be replaced by an automatic device.

Most of the cell screening automation equipment sold in the current market is a large-scale automatic screening system in Europe and America, which is expensive, and many technologies are technically blocked in China, so that the cell screening automation equipment is difficult to popularize domestically. Related companies and organizations in China have not developed the research and development technology of the equipment, and are mostly transformed and upgraded into the automatic focusing microscope on the basis of the existing manual focusing microscope. However, in the process of modification and upgrade, because the existing manual focusing microscope stage has small installation space and limited moving range, the automatic feeding of the stage becomes a technical problem. Related patents have also been reported in which two sets of vertically-distributed screw-nut transmission mechanisms are disposed under the object stage to realize automatic feeding of the object stage in the front-back and left-right directions. However, analysis shows that the automatic feeding mode has a usable value in the overall redesign and research of the automatic focusing microscope, but the automatic modification of the existing manual focusing microscope is not usable due to the limitation of the installation space and the moving range, which means that the existing manual focusing microscope will be scrapped, greatly increases the operation and maintenance cost of the medical institution, and causes resource waste.

In view of the above, the inventors considered that how to provide an automatic feeding device for a cell sorting microscope stage, particularly an automatic feeding device suitable for automatic modification of a conventional manual focusing cell sorting microscope, has become a long-standing technical demand of those skilled in the art.

Disclosure of Invention

In order to overcome the defects in the background technology, the invention provides the automatic feeding device for the cell screening microscope objective table, which has the characteristics of small volume, high precision and exquisite configuration, and is particularly suitable for automatically transforming the existing manual focusing cell screening microscope and the like.

In order to achieve the above purpose, the invention adopts the following technical scheme:

an automatic feeding device for an objective table of a cell screening microscope comprises an objective platform, a transverse feeding mechanism and a longitudinal feeding mechanism, wherein a guide rail B in the longitudinal feeding mechanism is arranged on the side edge of the objective platform, a rack A is arranged below the guide rail B, a slide block B is arranged in the guide rail B, a connecting frame is arranged on the slide block B, a supporting plate is arranged on the lower portion of the connecting frame, a stepping motor C and a stepping motor B are respectively arranged on the upper portion and the lower portion of the supporting plate, a belt wheel B and a belt wheel A are respectively arranged on main shafts of the stepping motor C and the stepping motor B, the belt wheel B and the belt wheel A are respectively connected with a belt wheel D arranged on the lower portion of an outer edge surface of a hollow gear shaft and a belt wheel C arranged on the outer edge surface of the main shaft through a transmission belt, the hollow gear shaft is sleeved on the outer edge surface of the lower, the automatic feeding device is characterized in that two ends of the main shaft are arranged on the supporting plate through flanges and bearing seats respectively, a gear is arranged at the upper end head of the main shaft, the gear is meshed with a rack B in the transverse feeding mechanism, the rack B is arranged below a sliding block A, the sliding block A is arranged in a guide rail A, the guide rail A is arranged on the connecting frame, and a bracket is arranged on the sliding block A to form the automatic feeding device for the cell screening microscope stage.

The automatic feeding device for the cell screening microscope objective table is characterized in that the stepping motor C is fixed on the upper portion of the connecting frame through the motor mounting plate A and is connected with the control system through a circuit.

The automatic feeding device for the cell screening microscope objective table is characterized in that the stepping motor B is fixed on the lower portion of the connecting frame through a motor mounting plate B and is connected with the control system through a circuit.

The automatic feeding device for the cell screening microscope stage is characterized in that the rear end part of the stage is fixed on a bracket of a microscope.

The automatic feeding device for the cell screening microscope objective table is characterized in that a bearing is arranged between the outer edge surface of the lower end head of the main shaft and the bearing seat.

The automatic feeding device for the cell screening microscope stage is characterized in that a 96-hole plate is arranged on the bracket.

The automatic feeding device for the cell screening microscope stage is characterized in that the transmission belt is any one of a synchronous belt, a multi-wedge belt or a triangular belt.

The automatic feeding device for the cell screening microscope objective table is characterized in that the belt wheel C is connected with the main shaft through a flat key, or a jackscrew hole is formed in the outer edge surface of the belt wheel C, and a jackscrew is arranged in the jackscrew hole.

The automatic feeding device for the cell screening microscope objective table is characterized in that a jackscrew hole is formed in the outer edge surface of the belt wheel D, and a jackscrew is arranged in the jackscrew hole.

The automatic feeding device for the cell screening microscope stage is characterized in that a bearing is arranged between the inner edge surface of the inner hole of the hollow gear shaft and the outer edge surface of the flange.

By adopting the technical scheme, the invention has the following advantages:

according to the invention, the transverse feeding mechanism and the longitudinal feeding mechanism are arranged on the objective platform, so that the objective platform can be automatically fed under the conditions of narrow installation space and limited moving range of the existing manual focusing microscope objective platform, powerful support is provided for the automatic modification of the existing manual focusing cell screening microscope, and the like.

Drawings

FIG. 1 is a schematic diagram of an application structure of the present invention;

FIG. 2 is a schematic structural view of the present invention;

FIG. 3 is a schematic perspective view of the spindle of the present invention;

FIG. 4 is a schematic front view of the structure of FIG. 3 according to the present invention;

FIG. 5 is a schematic sectional view A-A of FIG. 4 according to the present invention;

in the figure: 1. a light source; 2. a 96-well plate; 3. a serial interface; 4. a CCD; 5. a bracket; 6. a carrier platform; 7. an eyepiece; 8. a stepping motor A; 9. a base plate; 10. a stepping motor B; 11. a pulley A; 12. a synchronous belt A; 13. a belt pulley B; 14. a synchronous belt B; 15. an objective lens; 16. a stepping motor C; 17. a slide block A; 18. a guide rail A; 19. a main shaft; 20. a pulley C; 21. a pulley D; 22. a hollow gear shaft; 23. a rack A; 24. a flange; 25. a gear; 26. a guide rail B; 27. a slide block B; 28. a connecting frame; 29. a support plate; 30. a motor mounting plate A; 31. a motor mounting plate B; 32. a bearing seat; 33. a rack B; 34. and a bearing.

Detailed Description

The present invention will be explained in more detail by the following examples, which are not intended to limit the invention;

it should be noted that the directions or positional relationships indicated by "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc., used in describing the structure of the present invention are based on the directions or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and thus, should not be construed as limiting the present invention.

The automatic feeding device for the objective table of the cell screening microscope described in conjunction with fig. 1 to 5 comprises an objective table 6, a transverse feeding mechanism and a longitudinal feeding mechanism, wherein the rear end of the objective table 6 is fixed on the support of the microscope, it should be noted that the microscope is a conventional standard component, and therefore, the detailed structure of the microscope is not described in detail, a guide rail B26 in the longitudinal feeding mechanism is arranged at the side edge of the objective table 6, a rack a23 is arranged below the guide rail B26, a slide block B27 is arranged in the guide rail B26, a connecting frame 28 is arranged on the slide block B27, a support plate 29 is arranged at the lower part of the connecting frame 28, a stepping motor C16 and a stepping motor B10 are respectively arranged at the upper part and the lower part of the support plate 29, the stepping motor C16 is fixed at the upper part of the connecting frame 28 through a motor mounting plate a30, the stepping motor C16 is connected to a control system through a line, the stepping motor B10 is fixed at the lower part, the stepper motor B10 is wired to the control system, which is not described herein since the control system is not the focus of the present invention and since standard parts are readily available on the market by anyone skilled in the art. A belt wheel B13 and a belt wheel A11 are respectively arranged on the main shafts of the stepping motor C16 and the stepping motor B10, the belt wheel B13 and the belt wheel A11 are respectively connected with a belt wheel D21 arranged at the lower part of the outer edge surface of the hollow gear shaft 22 and a belt wheel C20 arranged on the outer edge surface of the main shaft 19 through a transmission belt, the transmission belt is any one of a synchronous belt, a multi-wedge belt or a triangular belt, in particular, in order to improve the running precision, the transmission belt is preferably a synchronous belt, namely, the belt wheel B13 and the belt wheel A11 are respectively connected with a belt wheel D21 arranged at the lower part of the outer edge surface of the hollow gear shaft 22 and a belt wheel C20 arranged on the outer edge surface of the main shaft 19 through; in specific implementation, the belt wheel C20 is fixedly connected with the main shaft 19 through a flat key, or a jackscrew hole is formed in the outer edge surface of the belt wheel C20, a jackscrew is arranged in the jackscrew hole, and the belt wheel C20 is fixed on the main shaft 19 through the jackscrew, and in specific implementation, a jackscrew groove can be formed in the position, corresponding to the jackscrew, of the outer edge surface of the main shaft 19, so that a fixing effect can be better achieved, and meanwhile, the flat key or the spline can be used for being combined with the jackscrew for fixing and the like; the hollow gear shaft 22 is sleeved on the outer edge surface of the lower portion of the flange 24, in implementation, a jackscrew hole is formed in the outer edge surface of the belt wheel D21, a jackscrew is arranged in the jackscrew hole, the belt wheel D21 and the hollow gear shaft 22 are fixed together through the jackscrew, even if the hollow gear shaft 22 and the belt wheel D21 rotate together, at the moment, the hollow gear shaft 22 can rotate around the flange 24, and in implementation, in order to improve the flexibility of rotation, a bearing is arranged between the inner edge surface of an inner hole of the hollow gear shaft 22 and the outer edge surface of the flange 24; the teeth of a cogwheel on the hollow gear axle 22 and rack A23 meshing, the both ends of main shaft 19 are passed through flange 24 and bearing frame 32 respectively and are set up on backup pad 29, be equipped with bearing 34 between the outer fringe face of main shaft 19 lower end and the bearing frame 32, be equipped with gear 25 at the upper end of main shaft 19, gear 25 and the meshing of rack B33 among the transverse feed mechanism, rack B33 sets up below slider A17, slider A17 sets up in guide rail A18, guide rail A18 sets up on link 28, is equipped with bracket 5 on slider A17, be equipped with 96 orifice plates 2 on bracket 5 and form the automatic feeding device who is used for cell screening microscope objective table.

When the invention is implemented specifically, a microscope is placed on the bottom plate 9, the loading platform 6 on the microscope is driven by the stepping motor A8 to realize the ascending and descending of the loading platform 6, biological cells to be detected are placed on the 96-hole plate 2, and the 96-hole plate 2 is placed on the bracket 5;

a stepping motor B10 drives a main shaft 19 to rotate through a belt wheel A11, a synchronous belt A12 and a belt wheel C20, the main shaft 19 transmits power to a sliding block A17 through a gear 25 and a rack B33, and the sliding block A17 drives a bracket 5 and a 96-hole plate 2 placed on the bracket 5 to transversely move to complete transverse feeding of a microscope objective table;

the hollow gear shaft 22 is driven by a stepping motor C16 through a belt wheel B13, a synchronous belt B14 and a belt wheel D21, the hollow gear shaft 22 is meshed with a rack A23 and is matched and restricted with a flange 24, a connecting frame 28, a sliding block B27 and a guide rail B26 in an installing way, so that the hollow gear shaft 22 drives the flange 24, the connecting frame 28, a supporting plate 29, a motor mounting plate A30, the stepping motor C16, a belt wheel B13, the synchronous belt B14, a belt wheel D21, the motor mounting plate B31, the stepping motor B10, the belt wheel A11, the synchronous belt A12, a belt wheel C20, a main shaft 19, a bearing seat 32, a bearing 34, the guide rail A18, the sliding block A17, the rack B33, a bracket 5 and a 96 orifice plate 2 to move longitudinally integrally, and further realize the longitudinal feeding of the.

Finally, the automatic feeding of the cell screening microscope stage is realized through the ingenious matching of the motion control of the stepping motor B10 and the stepping motor C16 and the transverse and longitudinal motion mechanisms.