阅读说明：本技术 外植体消毒装置 (Explant sterilizing device ) 是由 穆金艳 贾文庆 邱永杰 郭祥云 于 2021-07-15 设计创作，主要内容包括：本发明提供一种外植体消毒装置,涉及植物组织培养技术领域,包括容器、分隔装置和过滤装置。其中,容器的第一端设有开口。分隔装置设置于容器内以分隔出至少两个腔室,分隔装置的一端设置在容器的第二端,另一端朝开口延伸,各腔室靠近开口的一端均设有用于放置外植体的置入口。过滤装置可拆卸设置于容器内,且用于阻止各腔室内的外植体流失。可将各种类的外植体置入不同腔室内以同时消毒,提高处理效率。通过设置过滤装置,在将容器内的液体倒出时,能够避免腔室内的外植体随液体流失,以达到不浪费外植体的效果。在加入消毒液时,可以使消毒液液面超过过滤装置,以使外植体可以完全浸入到消毒液内,以达到对外植体进行全面消毒的效果。(The invention provides an explant disinfection device, and relates to the technical field of plant tissue culture. Wherein the first end of the container is provided with an opening. The separating device is arranged in the container to separate at least two chambers, one end of the separating device is arranged at the second end of the container, the other end of the separating device extends towards the opening, and one end of each chamber, which is close to the opening, is provided with an inlet for placing an explant. The filtering device is detachably arranged in the container and is used for preventing the explants in each chamber from losing. Various explants can be placed in different chambers to be disinfected simultaneously, and the treatment efficiency is improved. Through setting up filter equipment, when pouring the liquid in the container, can avoid the explant in the cavity to run off along with liquid to reach the effect of not extravagant explant. When the disinfectant is added, the liquid level of the disinfectant can exceed the filtering device, so that the explant can be completely immersed in the disinfectant, and the effect of comprehensively disinfecting the explant is achieved.)

1. An explant sterilization device, comprising: a container, a separating device and a filtering device;

wherein the first end of the container is provided with an opening; the separating device is arranged in the container to separate at least two chambers, one end of the separating device is arranged at the second end of the container, the other end of the separating device extends towards the opening, and one end of each chamber close to the opening is provided with a placement port for placing explants; the filtering device is detachably arranged in the container and is used for preventing the explants in the cavities from losing.

2. The explant sterilization device of claim 1, wherein the container is configured as a cylinder.

3. The explant sterilization device of claim 2, wherein the filtration device is in a disc-shaped configuration and is removably attached to the barrel by an attachment structure.

4. The explant sterilization device of claim 3, wherein the connection structure comprises a first locating structure for abutting a first end of the filtration device and a second locating structure for abutting a second end of the filtration device;

wherein, first location structure with second location structure all set up in on the inner wall of drum, and the two is followed the axial of drum distributes, first location structure with have between the second location structure and be greater than or equal to the clearance of filter equipment's thickness, filter equipment is equipped with the confession first location structure passes dodge the groove.

5. The explant sterilization device of claim 4, wherein the second positioning structure is provided with a positioning groove extending along the axial direction of the cylinder, the positioning groove is used for being in sliding fit with the separation device, and the length of the separation device along the axial direction of the cylinder is equal to the length of one end of the filtration device close to the bottom of the container from the bottom of the cylinder.

6. The explant sterilization device of claim 5, wherein the second positioning structure is provided as a raised structure disposed along the circumference of the cylinder, the positioning groove being provided on the raised structure.

7. The explant sterilization device of claim 4, wherein the first positioning structure is provided as at least two protrusions distributed along the circumference of the cylinder.

8. The explant sterilization device of claim 2, wherein the partitioning means comprises at least two partitions, each of which has a first end abutting the bottom of the cylinder and a second end extending towards the opening;

the first sides of the blocking parts are connected with each other, the second sides of the blocking parts extend along the radial direction of the cylinder, the space between any two adjacent blocking parts forms the chamber, and the gap between the second ends of any two adjacent blocking parts forms the placing opening.

9. The explant sterilization device of claim 1, wherein the partition means is provided with a communicating structure for communicating liquid in two adjacent chambers.

10. The explant sterilization device of claim 1, further comprising a lid for closing or opening the opening.

Technical Field

The invention relates to the technical field of plant tissue culture, in particular to an explant disinfection device.

Background

Plant tissue culture, also known as plant in vitro culture, refers to a technique of inoculating isolated plant organs, tissues, cells and protoplasts to a suitable culture medium under aseptic environment conditions to regenerate the plant into a complete plant. These isolated plant organs, tissues, cells, etc. are inoculated with materials, all referred to as explants.

The explant needs to be disinfected in the process of plant tissue culture, and the disinfection method generally adopted in the prior art is to put the explant into a beaker, pour disinfection alcohol into the beaker, pour the disinfection alcohol out after 30-40 seconds, then pour disinfectant such as sodium hypochlorite solution or mercury bichloride into the beaker, pour the disinfectant out after 10-20 minutes, and then rinse the explant with sterile water for 3 times. The disinfection solution and the sterile water need to be replaced for at least 5 times in the whole disinfection process, for explants with small volume and light weight, the explants are easy to run off along with the pouring of the liquid when the disinfection solution and the sterile water are replaced each time, and the explants float on the surface of the disinfection solution and cannot be completely immersed in the disinfection solution, so that the explants are not disinfected thoroughly. In addition, the above sterilization methods cannot treat multiple explants simultaneously.

Therefore, the technical problem to be solved by the technical staff in the art is how to solve the problems that the method for disinfecting the explant in the prior art cannot treat multiple explants simultaneously, and the explant is easy to float and lose in the treatment process.

Disclosure of Invention

The invention provides an explant disinfection device which can treat various explants at the same time, can ensure that the explants are completely immersed in a disinfectant and cannot run off along with the pouring of liquid, and aims to overcome the defects that the explant disinfection method in the prior art cannot treat various explants at the same time, and the explants are easy to float and run off in the treatment process, realize the disinfection treatment of various explants at the same time, improve the disinfection efficiency, have thorough disinfection effect and do not waste the explant effect.

The invention provides an explant sterilization device, comprising: a container, a separating device and a filtering device;

wherein the first end of the container is provided with an opening; the separating device is arranged in the container to separate at least two chambers, one end of the separating device is arranged at the second end of the container, the other end of the separating device extends towards the opening, and one end of each chamber close to the opening is provided with a placement port for placing explants; the filtering device is detachably arranged in the container and is used for preventing the explants in the cavities from losing.

According to the explant sterilization device provided by the invention, the container is arranged into a cylinder.

According to the explant sterilization device provided by the invention, the filtering device is in a disc-shaped structure and is detachably connected with the cylinder through a connecting structure.

According to the explant sterilization device provided by the invention, the connecting structure comprises a first positioning structure used for abutting against a first end of the filtering device and a second positioning structure used for abutting against a second end of the filtering device;

wherein, first location structure with second location structure all set up in on the inner wall of drum, and the two is followed the axial of drum distributes, first location structure with have between the second location structure and be greater than or equal to the clearance of filter equipment's thickness, filter equipment is equipped with the confession first location structure passes dodge the groove.

According to the explant sterilization device provided by the invention, the second positioning structure is provided with a positioning groove which extends along the axial direction of the cylinder and is used for being in sliding fit with the separation device, and the length of the separation device along the axial direction of the cylinder is equal to the length of one end, close to the bottom of the container, of the filtration device, and the length of the end, close to the bottom of the container, of the filtration device is equal to the length of the bottom of the cylinder.

According to the explant sterilization device provided by the invention, the second positioning structure is provided with a convex structure along the circumferential direction of the cylinder, and the positioning groove is arranged on the convex structure.

According to the explant sterilization device provided by the invention, the first positioning structure is provided with at least two lugs distributed along the circumferential direction of the cylinder.

According to the explant sterilization device provided by the invention, the partition device comprises at least two partitions, the first end of each partition is abutted against the bottom of the cylinder, and the second end of each partition extends towards the opening;

the first sides of the blocking parts are connected with each other, the second sides of the blocking parts extend along the radial direction of the cylinder, the space between any two adjacent blocking parts forms the chamber, and the gap between the second ends of any two adjacent blocking parts forms the placing opening.

According to the invention, an explant sterilization device is provided, and the separation device is provided with a communication structure for communicating liquid in two adjacent chambers.

According to the invention, the explant sterilization device further comprises a cover body for plugging or opening the opening.

According to the explant sterilization device provided by the invention, the internal space of the container can be divided into at least two chambers by arranging the separation device, and different types of explants can be placed into different chambers in the sterilization process, so that the effect of simultaneously sterilizing various explants is achieved, the treatment efficiency of the explants is improved, and the consistent sterilization time of various explants can be ensured. And through setting up filter equipment, when pouring the liquid in the container out, can avoid the explant in the cavity to run off along with liquid on the one hand to reach the effect of not extravagant explant. On the other hand, when the disinfection solution is added, the liquid level of the disinfection solution can be higher than that of the filtering device, and under the blocking effect of the filtering device, the explant can be completely immersed into the disinfection solution, so that the effect of comprehensively disinfecting the explant is achieved.

Drawings

In order to more clearly illustrate the present invention or technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is a schematic structural view of an explant sterilization device provided by the present invention;

FIG. 2 is an exploded view of the structure of the explant sterilization device provided by the present invention;

FIG. 3 is a schematic structural view of a first partitioning apparatus provided in the present invention;

FIG. 4 is a schematic structural view of a second partitioning apparatus provided in the present invention;

FIG. 5 is a schematic view of a third embodiment of the partition according to the present invention;

reference numerals:

1: a container; 2: a filtration device; 3: an avoidance groove;

4: a bump; 5: a raised structure; 6: a barrier portion;

7: a cover body; 8: a chamber.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

An explant sterilization device in an embodiment of the present invention is described below with reference to fig. 1 to 5.

In particular, the explant sterilisation device comprises a container 1, separation means and filtration means 2. Wherein the first end of the container 1 is provided with an opening. A partition is provided within the container 1 to separate at least two chambers 8 within the container 1. One end of the partition means is arranged at the second end of the container 1 and the other end extends towards the opening of the container 1, so that each chamber 8 extends in the direction from the second end to the first end of the container 1. And one end of each chamber 8 close to the opening is provided with an inlet for placing explants. The filter device 2 is used to prevent the loss of explants in each chamber 8, and the filter device 2 is removably arranged inside the container 1.

The explant sterilization device provided by the embodiment of the invention can divide the internal space of the container 1 into at least two chambers 8 by arranging the separation device, and different types of explants can be placed into different chambers 8 in the sterilization process, so that the effect of simultaneously sterilizing various explants is achieved, the treatment efficiency of the explants is improved, and the consistent sterilization time of various explants can be ensured. And through setting up filter equipment 2, when pouring out the liquid with in the container 1 on the one hand, can avoid the explant in the cavity 8 to run off along with the liquid to reach the effect of not extravagant explant. On the other hand, when the disinfection solution is added, the liquid level of the disinfection solution can be higher than that of the filtering device 2, and under the blocking effect of the filtering device 2, the explants can be completely immersed into the disinfection solution, so that the effect of comprehensively disinfecting the explants is achieved.

The specific using process is as follows: firstly, suitable separation devices are arranged in the container 1 according to the number of the types of explants, then corresponding explants are placed in each chamber 8, and finally the filtering device 2 is installed. Then, sterile alcohol is first poured into the container 1, which is required to submerge the filtering device 2, so that the explants are completely immersed in the sterile solution. Pouring out the sterilized alcohol after a certain time, then pouring in sodium hypochlorite or mercuric chloride solution, and finally washing with sterile water, wherein the steps of the method are the same as above. After the disinfection is finished, the filtering device 2 is taken out, and then the tweezers are used for clamping the explants in the cavity 8 to the sterilized absorbent paper to absorb water for later use.

In some embodiments of the invention, the container 1 is provided as a cylinder. So, the drum is convenient for manufacturing and is convenient for the experimenter to hold.

In some embodiments of the invention, the filter device 2 is in a disc-shaped configuration and the filter device 2 is removably connected to the cylinder by a connection structure. So set up, be convenient for carry out the dismouting to filter equipment 2.

In some embodiments of the invention, the connecting structure includes a first locating structure and a second locating structure. The first positioning structure is used for abutting against the first end of the filtering device 2, and the second positioning structure is used for abutting against the second end of the filtering device 2. The first positioning structure and the second positioning structure are both arranged on the inner wall of the cylinder and are distributed along the axial direction of the cylinder. A gap is arranged between the first positioning structure and the second positioning structure, and the width of the gap is larger than or equal to the thickness of the filter device 2. The filter device 2 is provided with an avoiding groove 3 for the first positioning structure to pass through. For example, when the filter device 2 is installed, the avoiding groove 3 on the filter device 2 may be opposite to the first positioning structure, so that the filter device 2 enters between the first positioning structure and the second positioning structure, and then the filter device 2 is rotated by a certain angle, so that the first positioning structure and the avoiding groove 3 are staggered. So, soak disinfection in-process second location structure and can play the effect that supports filter equipment 2, prevent that filter equipment 2 from moving down. When empting the liquid in the drum, first location structure can play the effect of supporting filter equipment 2, can prevent that filter equipment 2 from droing. When the filter device 2 is disassembled, the filter device 2 is firstly rotated until the avoiding groove 3 is opposite to the first positioning structure, and then the filter device 2 is taken out. So set up, connection structure's simple structure to filter equipment 2's installation and dismantlement are all convenient and fast.

In some embodiments of the invention, the second positioning structure is provided with a positioning groove extending along the axial direction of the cylinder, and the separating device is in sliding fit with the positioning groove. And the length of the separating device along the axial direction of the cylinder is equal to the length from one end of the filtering device close to the bottom of the container to the bottom of the cylinder. When the separating device is used, the separating device slides into the container 1 along the positioning groove, and as shown in fig. 1, after the separating device is arranged in the container 1, the positioning groove can play a role in supporting and positioning the separating device, so that the stability of the separating device in the container 1 is improved, and the separating device is prevented from moving in the container 1. And the axial length of the separating device along the cylinder is equal to the length from one end of the filtering device close to the bottom of the container to the bottom of the cylinder, so that the separating device can be attached to the filtering device, and the explants in the cavities are prevented from entering other cavities and being mixed with other explants.

In some embodiments of the invention the second locating formations are provided as raised formations 5 provided along the circumference of the cylinder. The positioning groove is arranged on the convex structure 5. Further, the number of the protruding structures 5 may be multiple, and a gap between two adjacent protruding structures 5 forms a positioning groove, or the protruding structures 5 may also be ring structures, and the positioning groove is a gap formed in the ring structures.

In some embodiments of the invention, the first locating structure is provided as at least two lugs 4 distributed along the circumference of the cylinder. Correspondingly, the number of the avoiding grooves 3 on the filtering device 2 is the same as that of the bumps 4, and the avoiding grooves and the bumps are in one-to-one correspondence.

In some embodiments of the invention, the separating means comprises at least two baffles 6. The first end of each baffle part 6 is abutted against the bottom of the cylinder, and the second end of each baffle part extends towards the opening. The first sides of the respective barriers 6 are interconnected and the second sides are arranged extending in the radial direction of the cylinder. The space between any two adjacent baffles 6 forms a chamber 8, and the gap between the second ends of any two adjacent baffles 6 forms an inlet. Alternatively, the height of each barrier 6 may be set to be two thirds of the height of the container 1. Further, the second side of each barrier 6 may extend into the corresponding positioning slot. As shown in fig. 1 to 3, an example in which the number of the baffle portions 6 is two is shown, and in this case, the number of the chambers 8 is two. The two barriers 6 may be of one-piece construction, i.e. the two barriers are each part of one-piece construction. As shown in fig. 4, an example is shown in which the number of the baffle portions 6 is three, and in this case, the number of the chambers 8 is three. As shown in fig. 5, the number of the baffle portions 6 is four, and the number of the chambers 8 is four. Of course, the number of the baffle parts 6 can also be set according to actual needs.

In some embodiments of the invention, the separating means is provided with communicating means for communicating the liquid in two adjacent chambers 8. With this arrangement, the sterilizing liquid and the like can be uniformly disposed in all the chambers 8, so that the explants in each chamber 8 are subjected to the same sterilizing conditions. Further, the barrier 6 may be a screen, and the communicating structure is a mesh. Of course, the barrier 6 is not limited to being a screen, and for example, the barrier 6 may be a partition plate, and the communicating structure may be a through hole provided in the partition plate.

In some embodiments of the invention, the explant sterilisation device further comprises a lid 7 for closing off or opening the opening. Through setting up lid 7, usable lid 7 comes the shutoff opening in disinfection process for the experimenter can rock container 1, and it has the bubble to bond on avoiding the explant, so that the explant contacts with the antiseptic solution more fully, and so, to the disinfection of explant more thoroughly, lid 7 can avoid rocking container 1 in-process liquid outflow. Optionally, the cap 7 includes, but is not limited to, being connected to the container 1 by means of a threaded connection.

Further, the filter device 2 may be a screen or a partition provided with through holes.

Furthermore, the container 1, the filtering device 2, the blocking portion 6 and the cover 7 may be made of glass or PVC, which is resistant to acid, alkali and high temperature sterilization.

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various modifications and substitutions can be made without departing from the technical principles of the present invention, and these modifications and substitutions should also be considered as the scope of the present invention.