阅读说明：本技术 从固定化细胞或ffpe组织切片脱离增强抗原性的细胞核的方法以及用于该方法的抗原活化剂及试剂盒 (Method for detaching antigenicity-enhanced cell nucleus from immobilized cell or FFPE tissue section, and antigen activator and kit used for method ) 是由 佐藤七月 黑岩美佳 中辻匡俊 石原英幹 于 2019-02-28 设计创作，主要内容包括：本发明提供用于使用Ki-67抗体检测含Ki-67蛋白质阳性细胞核的细胞的数的预处理方法、该检测方法、该检测方法中使用的试剂盒、及使用该方法的治疗方案选定。探讨由不旨在Ki-67抗原的活化的酶的活化,用不识别MIB-1的表位的酶(稀有切点酶)预处理试样,则含细胞角蛋白的其他抗原的抗原性也增强的同时,增强Ki-67抗原的抗原活化。结果,从FFPE切片增强抗原性的状态下使细胞核脱离,以存在于该核的Ki-67蛋白质作为目标,通过使荧光标记的抗体反应,完成客观性、再现性、普遍性更高的Ki-67阳性细胞的定量方法。(The present invention provides a pretreatment method for detecting the number of cells containing a Ki-67 protein-positive nucleus using a Ki-67 antibody, the detection method, a kit used in the detection method, and selection of a treatment regimen using the method. To investigate the activation of an enzyme not intended for the activation of the Ki-67 antigen, when a sample was pretreated with an enzyme that does not recognize an epitope of MIB-1 (rare-cutting enzyme), the antigenicity of another antigen containing cytokeratin was also increased, and the antigen activation of the Ki-67 antigen was also increased. As a result, the cell nucleus was detached from the FFPE section with the antigenicity enhanced, and the Ki-67 protein present in the nucleus was targeted, and a fluorescence-labeled antibody was reacted to complete a method for quantifying Ki-67-positive cells with higher objectivity, reproducibility, and universality.)