阅读说明：本技术 一种对喷浆玉米胚芽柏中赤霉烯酮毒素的生物降解方法 (Biodegradation method for gibberellin ketone toxin in spray-painted maize germ cypress ) 是由 张玉国 张楠楠 于 2019-04-17 设计创作，主要内容包括：本发明公开了一种对喷浆玉米胚芽柏中赤霉烯酮毒素的生物降解方法,采用物理法与生物降解法结合的方式对玉米胚芽中赤霉烯酮毒素有效的清毒。该发明,首先进行了玉米胚芽的除杂操作,并随之进行物理清毒,能够确保玉米胚芽纯度,同时大大降低了玉米胚芽中的赤霉烯酮含量,大大加强了后续生物降解过程的效果,利用酿酒酵母的降解酶进行赤霉烯酮毒素降解,配合德氏乳杆菌的细胞壁吸附特性能够清除玉米胚芽中97.8%的赤霉烯酮毒素,确保了食品安全。(The invention discloses a biodegradation method for gibberellin ketene toxin in guniting maize germ cypress, which adopts a mode of combining a physical method and a biodegradation method to effectively detoxify the gibberellin ketene toxin in the maize germ. According to the method, firstly, the corn germ is subjected to impurity removal operation, and then physical detoxification is carried out, so that the purity of the corn germ can be ensured, the content of the gibberellin in the corn germ is greatly reduced, the effect of a subsequent biodegradation process is greatly enhanced, the gibberellin toxin is degraded by using degrading enzyme of saccharomyces cerevisiae, 97.8% of the gibberellin toxin in the corn germ can be removed by matching with the cell wall adsorption characteristic of lactobacillus delbrueckii, and the food safety is ensured.)

1. A biodegradation method for gibberellin ketene toxin in spray-painted maize germ cypress is characterized by comprising the following steps:

s1, taking corn germ to be processed, carrying out water separation to remove impurities, and filtering corn germ water for later use;

s2, crushing the maize germ in the S1, and performing primary physical detoxification on the crushed maize germ under the conditions of 155-179 ℃ and steam pressure of 0.8-1.2MPa to obtain a primary maize germ detoxification product;

s3 Saccharomyces cerevisiae culture:

(1) firstly, culturing for 48 hours in a solid culture medium, then taking a single colony, and inoculating the single colony into a liquid culture medium for shake culture;

(2) extracting a beer yeast culture product zearalenone degrading enzyme, culturing the product at 37 ℃ for 3-5 days by using beer yeast, taking a culture solution, freezing and centrifuging for 20min under the conditions of 4 ℃ and 7000-8000 r/min, or separating a supernatant and thallus cells by adopting a suction filtration method, taking the separated supernatant to obtain an extracellular crude extract, precipitating by using 30-90% ammonium sulfate, freezing and centrifuging for 10-20min under the conditions of 4 ℃ and 8000 r/min, taking a precipitate, and freezing and drying to obtain a refined zearalenone degrading enzyme;

s4 proliferation culture of Lactobacillus delbrueckii: inoculating the activated strain into proliferation culture medium at a ratio of 5-6% (V/V), and culturing at 37 deg.C for 10 hr to obtain culture solution; collecting thallus, and centrifuging the culture solution at 4 ℃ and 6000-7000r/mim for 10-15min to obtain bacterial sludge;

s5, mixing zearalenone degrading enzyme obtained in S3 and bacterial sludge in S4, mixing the mixture of the zearalenone degrading enzyme and the bacterial sludge with the primary detoxified product of the maize germ in S2, placing the mixture into a centrifugal dehydrator for centrifugal processing at the rotating speed of 4200-.

2. The method of claim 1, wherein the method comprises the steps of: the solid culture medium in the step (1) of the step S1 comprises 5-15% of corn flour and 70-90% of bran by weight.

3. The method of claim 1, wherein the method comprises the steps of: the step (1) of the step S1 comprises the steps of culturing at the temperature of 30-40 ℃, culturing for 24-40 h, adjusting the pH value to 5.0-9.0 and rotating at the speed of 200-300 r/min.

4. The method of claim 1, wherein the method comprises the steps of: the composition of the liquid medium in the step (1) of step S1 is 1% Yeast Extract (Yeast Extract), 2% Peptone (Peptone), 2% Dextrose.

5. The method of claim 1, wherein the method comprises the steps of: and in the step S2, softening operation is carried out on the crushed maize germs, wherein the softening temperature is controlled to be 75-80 ℃, and the moisture of the softened maize germs is controlled to be 15-20%.

Technical Field

The invention relates to the technical field of biological detoxification, in particular to a biodegradation method for gibberellin ketene toxin in spray-dried maize germ cypress.

Background

Zearalenone (ZEN), a mycotoxin produced mainly by fusarium fungi, is widely found in corn, barley, wheat and sorghum grains and by-products thereof, has an estrogenic effect and acts mainly on the reproductive system, thus causing hypermenorrhea in livestock, poultry and laboratory mice. Consumption of zearalenone containing foods by pregnant animals (including humans) can cause abortion, stillbirth and teratogenesis. The food made from wheat flour containing gibberellic disease can also cause poisoning symptoms of central nervous system, such as nausea, chill, headache, mental depression and ataxia.

Traditional food toxin removing methods are divided into physical methods and chemical methods, wherein the physical methods can remove part of toxins but destroy fifteen nutrients; the chemicals used in chemical processes may cause an uncertain hazard to food.

For food safety, the elimination of Zearalenone (ZEN) must ensure that the nutritional characteristics of foodstuffs, feedstuffs and human food are not altered; the zearalenone can be quickly and effectively removed; does not produce residues of toxic substances or carcinogenic/mutagenic residues; economically viable, biodegradation has been studied over the years as a means of effecting the decontamination of Zearalenone (ZEN).

Disclosure of Invention

Based on the technical problems in the background art, the invention provides a biodegradation method for the zearalenone toxin in the guniting maize germ, and solves the problems in the background art.

The invention provides the following technical scheme: a biodegradation method for gibberellin ketene toxin in spray-painted maize germ cypress, comprising the following steps:

s1, taking corn germ to be processed, carrying out water separation to remove impurities, and filtering corn germ water for later use;

s2, crushing the maize germ in the S1, and performing primary physical detoxification on the crushed maize germ under the conditions of 155-179 ℃ and steam pressure of 0.8-1.2MPa to obtain a primary maize germ detoxification product;

s3 culturing Saccharomyces cerevisiae;

(1) firstly, culturing for 48 hours in a solid culture medium, then taking a single colony, and inoculating the single colony into a liquid culture medium for shake culture;

(2) extracting a beer yeast culture product zearalenone degrading enzyme, culturing the product at 37 ℃ for 3-5 days by using beer yeast, taking a culture solution, freezing and centrifuging for 20min under the conditions of 4 ℃ and 7000-8000 r/min, or separating a supernatant and thallus cells by adopting a suction filtration method, taking the separated supernatant to obtain an extracellular crude extract, precipitating by using 30-90% ammonium sulfate, freezing and centrifuging for 10-20min under the conditions of 4 ℃ and 8000 r/min, taking a precipitate, and freezing and drying to obtain a refined zearalenone degrading enzyme;

s4 proliferation culture of Lactobacillus delbrueckii: inoculating the activated strain into proliferation culture medium at a ratio of 5-6% (V/V), and culturing at 37 deg.C for 10 hr to obtain culture solution; collecting thallus, and centrifuging the culture solution at 4 ℃ and 6000-7000r/mim for 10-15min to obtain bacterial sludge;

s5, mixing zearalenone degrading enzyme obtained in S3 and bacterial sludge in S4, mixing the mixture of the zearalenone degrading enzyme and the bacterial sludge with the primary detoxified product of the maize germ in S2, placing the mixture into a centrifugal dehydrator for centrifugal processing at the rotating speed of 4200-.

Preferably, the solid culture medium in the step (1) of the step S1 consists of 5-15% of corn flour and 70-90% of bran by weight.

Preferably, the shaking culture conditions of step (1) of step S1 are that the culture temperature is 30-40 ℃, the culture time is 24-40 h, the pH value is 5.0-9.0, and the rotation speed is 200-300 r/min.

Preferably, the composition of the liquid medium in the step (1) of step S1 is 1% Yeast Extract (Yeast Extract), 2% Peptone (Peptone), 2% Dextrose (glucose).

Preferably, the step S2 is to soften the crushed corn germ, wherein the softening temperature is controlled at 75-80 ℃, and the moisture content of the softened corn germ is controlled at 15-20%.

The invention provides a biodegradation method for gibberellin ketone toxin in guniting maize germ, which comprises the steps of firstly carrying out impurity removal operation on maize germs and then carrying out physical detoxification, ensuring the purity of the maize germs, simultaneously greatly reducing the content of the gibberellin ketone in the maize germs, greatly enhancing the effect of the subsequent biodegradation process, utilizing degrading enzyme of saccharomyces cerevisiae to carry out degradation on the gibberellin ketone toxin, and being matched with the cell wall adsorption characteristic of lactobacillus delbrueckii to remove 97.8% of the gibberellin ketone toxin in the maize germs, thereby ensuring the food safety.

Detailed Description

The technical solution of the present invention will be described in detail below with reference to specific examples.

Saccharomyces cerevisiae is also known as Saccharomyces cerevisiae, also known as Saccharomyces cerevisiae or Saccharomyces cerevisiae. Saccharomyces cerevisiae is the most widely related yeast to human, not only because it is traditionally used for making bread, steamed bread and other food and brewing wine, but also as a eukaryotic model organism in modern molecular and cellular biology, and its function is equivalent to prokaryotic model organism Escherichia coli. Saccharomyces cerevisiae is the most commonly used biological species in fermentation. The cells of Saccharomyces cerevisiae are spherical or ovoid, 5-10 μm in diameter. The propagation method is budding reproduction, the cell walls of yeasts such as saccharomyces cerevisiae and the like can better adsorb and remove ZEN, and the maximum adsorption capacity can reach 2.2 g/KG.