阅读说明：本技术 一种对玉米浆中赤霉烯酮毒素的生物降解方法 (Biodegradation method for gibberellin ketene toxin in corn steep liquor ) 是由 张玉国 张楠楠 于 2019-04-17 设计创作，主要内容包括：本发明公开了一种对玉米浆中赤霉烯酮毒素的生物降解方法,采用物理吸附法与生物降解法结合的方式进行玉米浆内赤霉烯酮毒素的清除,同时通过降低玉米浆含水量来提高清毒相率。本发明,通过降低玉米桨的水分含量,提高了玉米浆清毒效率,采用活性炭对玉米浆内赤霉烯酮毒素进行吸附,初步降低了玉米浆内赤霉烯酮毒素含量,进一步的采用生物降解的方式对玉米浆内的赤霉烯酮毒素进行降解,采用物理吸附法与生物降解法结合的二步法进行玉米浆内赤霉烯酮毒素的清除,相比于传统的清毒方式,该方案有效的提高了玉米浆内赤霉烯酮毒素的清除效率,大大降低了玉米浆清毒后的赤霉烯酮毒素含量。(The invention discloses a biodegradation method for zearalenone toxin in corn steep liquor, which is used for removing the zearalenone toxin in the corn steep liquor by combining a physical adsorption method and a biodegradation method, and simultaneously improves the toxin removal phase rate by reducing the water content of the corn steep liquor. According to the invention, the water content of the corn steep liquor is reduced, the corn steep liquor detoxification efficiency is improved, the activated carbon is adopted to adsorb the gibberellin ketone toxin in the corn steep liquor, the content of the gibberellin ketone toxin in the corn steep liquor is preliminarily reduced, the gibberellin ketone toxin in the corn steep liquor is further degraded in a biodegradation mode, and the removal of the gibberellin ketone toxin in the corn steep liquor is carried out in a two-step method combining a physical adsorption method and a biodegradation method.)

1. A method for biodegrading a zearalenone toxin in corn steep liquor, comprising the steps of:

s1, removing corn steep liquor and dehydrating, wherein a centrifugal dehydrator is adopted to dehydrate the corn steep liquor, the rotating speed is 3000-4000r/min, the dehydrating temperature is 80-100 ℃, the dehydrating time is 2-3h, the humidity of the dehydrated corn steep liquor is controlled at 30%, and the dehydrated corn steep liquor is in a low water slurry state;

s2, mixing activated carbon in the low-water slurry corn steep liquor obtained in the step S1, mixing the activated carbon with an activating agent in advance, then primarily mixing the activated carbon with the activating agent, taking solid matter, mixing, wherein the mixing rotation speed is 3000-;

s3, culturing saccharomyces cerevisiae by using a solid culture medium, culturing the saccharomyces cerevisiae at 37 ℃ for 3-5 days, taking a culture solution, freezing and centrifuging for 20min under the conditions of 4 ℃ and 7000-8000 r/min, or separating a supernatant from thallus cells by adopting a suction filtration method, taking the separated supernatant to obtain an extracellular crude extract, precipitating by using 30-90% ammonium sulfate, freezing and centrifuging for 10-20min under the conditions of 4 ℃ and 8000 r/min, taking a precipitate, and freezing and drying to obtain the refined zearalenone degrading enzyme;

s4, mixing the zearalenone degrading enzyme in the step S3 with the primary detoxified corn steep liquor in the step S2, and mixing by adopting a mixer at the mixing speed of 100-200r/min, the mixing temperature of 37-45 ℃ and the mixing time of 20-100h to obtain a detoxified product of the corn steep liquor;

s5, mixing the detoxified product obtained in the step S4 with pure water at a mixing ratio of 1:1, a mixing rotation speed of 100-;

s6, placing the corn steep liquor diluent in the step S5 into a centrifugal dehydrator for centrifugal dehydration at the rotating speed of 4200-.

2. The method of claim 1, wherein the zearalenone toxin is selected from the group consisting of: the solid culture medium in the step S2 comprises 5-15% of corn flour and 70-90% of bran by weight.

3. The method of claim 1, wherein the zearalenone toxin is selected from the group consisting of: the activators in said step S2 are cationic surfactants cetyl pyridinium and N, N-dimethyl-N-octadecanoyl benzyl ammonium.

4. The method of claim 1, wherein the zearalenone toxin is selected from the group consisting of: the mixing ratio of the activated carbon to the corn steep liquor in the S2 is 1: 5, the particle size range of the activated carbon particles is 1-5 mm.

Technical Field

The invention relates to the technical field of biological detoxification, in particular to a biodegradation method for zearalenone toxin in corn steep liquor.

Background

Zearalenone is a non-steroidal mycotoxin with estrogen-like effect, and is synthesized and secreted mainly by fusarium graminearum, fusarium roseum, fusarium moniliforme, fusarium tricuspidatum and the like.

Traditional food toxin removing methods are divided into physical methods and chemical methods, wherein the physical methods can remove part of toxins but destroy fifteen nutrients; the chemicals used in chemical processes may cause an uncertain hazard to food.

With the development of biotechnology, techniques for removing zearalenone toxins by biodegradation methods are widely applied, the common biodegradation methods are performed by degrading enzymes extracted from yeast, the removal of toxins is limited, and the removal effect of zearalenone toxins is not ideal for the treatment substance with high water content, such as corn steep liquor.

Disclosure of Invention

Based on the technical problems in the background art, the invention provides a biodegradation method for zearalenone toxin in corn steep liquor, which aims at performing two-step detoxification operation on the corn steep liquor and solves the problem that the removal of toxin is limited by a common biodegradation method.

The invention provides the following technical scheme: a method for biodegrading a zearalenone toxin in corn steep liquor, the method comprising the steps of:

s1, removing corn steep liquor and dehydrating, wherein a centrifugal dehydrator is adopted to dehydrate the corn steep liquor, the rotating speed is 3000-4000r/min, the dehydrating temperature is 80-100 ℃, the dehydrating time is 2-3h, the humidity of the dehydrated corn steep liquor is controlled at 30%, and the dehydrated corn steep liquor is in a low water slurry state;

s2, mixing activated carbon in the low-water slurry corn steep liquor obtained in the step S1, mixing the activated carbon with an activating agent in advance, then taking solid matter, mixing, wherein the mixing rotation speed is 3000-.

S3, culturing saccharomyces cerevisiae by using a solid culture medium, culturing the saccharomyces cerevisiae at 37 ℃ for 3-5 days, taking a culture solution, freezing and centrifuging for 20min under the conditions of 4 ℃ and 7000-8000 r/min, or separating a supernatant from thallus cells by adopting a suction filtration method, taking the separated supernatant to obtain an extracellular crude extract, precipitating by using 30-90% ammonium sulfate, freezing and centrifuging for 10-20min under the conditions of 4 ℃ and 8000 r/min, taking a precipitate, and freezing and drying to obtain the refined zearalenone degrading enzyme;

s4, mixing the zearalenone degrading enzyme in the step S3 with the primary detoxified corn steep liquor in the step S2, and mixing by adopting a mixer at the mixing speed of 100-200r/min, the mixing temperature of 37-45 ℃ and the mixing time of 20-100h to obtain a detoxified product of the corn steep liquor;

s5, mixing the detoxified product obtained in the step S4 with pure water at a mixing ratio of 1:1, a mixing rotation speed of 100-;

s6, placing the corn steep liquor diluent in the step S5 into a centrifugal dehydrator for centrifugal dehydration at the rotating speed of 4200-.

Preferably, the solid medium in the step S2 comprises 5-15% of corn flour and 70-90% of bran by weight.

Preferably, the activating agents in the step S2 are cationic surfactants cetyl pyridinium and N, N-dimethyl-N-octadecyl benzyl ammonium, and the activating agents can reduce the surface hydrophobicity of the activated carbon and improve the adsorption capacity of the activated carbon.

Preferably, the mixing ratio of the activated carbon and the corn steep liquor in the S2 is 1: 5, the particle size range of the activated carbon particles is 1-5 mm.

Compared with the prior art

According to the invention, the water content of the corn steep liquor is reduced, the corn steep liquor detoxification efficiency is improved, the activated carbon is adopted to adsorb the gibberellin ketone toxin in the corn steep liquor, the content of the gibberellin ketone toxin in the corn steep liquor is preliminarily reduced, the gibberellin ketone toxin in the corn steep liquor is further degraded in a biodegradation mode, and the removal of the gibberellin ketone toxin in the corn steep liquor is carried out in a two-step method combining a physical adsorption method and a biodegradation method.

Detailed Description

The technical solution of the present invention will be described in detail below with reference to specific examples.