阅读说明：本技术 一种多功能生物电化学污染物检测装置 (Multifunctional bioelectrochemical pollutant detection device ) 是由 成少安 林朱凡 于 2021-06-11 设计创作，主要内容包括：本发明公开了一种多功能生物电化学污染物检测装置,包括具有两腔室结构的反应器,该反应器包括阳极室和阴极室,阳极室和阴极室之间用阳离子交换膜隔开,在阴极室内设有非生物电极,在阳极室内设有参比电极和生物电极；当所述多功能生物电化学污染物检测装置中的生物电极和非生物电极外接电阻构成回路时,该电阻与反应器构建成生物电极培养装置；或,当所述多功能生物电化学污染物检测装置中的生物电极、非生物电极和参比电极以三电极体系连接恒电位仪时,该反应器与恒电位仪构建成生物电化学传感器一体化装置；其中,生物电化学传感器一体化装置所需的生物电极在生物电极培养装置中培养成熟。(The invention discloses a multifunctional bioelectrochemistry pollutant detection device, which comprises a reactor with a two-chamber structure, wherein the reactor comprises an anode chamber and a cathode chamber, the anode chamber and the cathode chamber are separated by a cation exchange membrane, a non-biological electrode is arranged in the cathode chamber, and a reference electrode and a biological electrode are arranged in the anode chamber; when a biological electrode and a non-biological electrode in the multifunctional bioelectrochemical pollutant detection device are externally connected with a resistor to form a loop, the resistor and a reactor form a biological electrode culture device; or when the bioelectrode, the non-bioelectrode and the reference electrode in the multifunctional bioelectrochemical pollutant detection device are connected with the potentiostat in a three-electrode system, the reactor and the potentiostat form a bioelectrochemical sensor integrated device; wherein, the bioelectrode required by the bioelectrochemical sensor integrated device is cultured and matured in the bioelectrode culture device.)

1. A multifunctional bioelectrochemical pollutant detection device is characterized in that: the reactor comprises a reactor with a two-chamber structure, wherein the reactor comprises an anode chamber and a cathode chamber, the anode chamber and the cathode chamber are separated by a cation exchange membrane, a non-biological electrode is arranged in the cathode chamber, and a reference electrode and a biological electrode are arranged in the anode chamber;

when a biological electrode and a non-biological electrode in the multifunctional bioelectrochemical pollutant detection device are externally connected with a resistor to form a loop, the resistor and a reactor form a biological electrode culture device; or the like, or, alternatively,

when a biological electrode, a non-biological electrode and a reference electrode in the multifunctional bioelectrochemical pollutant detection device are connected with a potentiostat in a three-electrode system, the reactor and the potentiostat form a bioelectrochemical sensor integrated device;

wherein, the bioelectrode required by the bioelectrochemical sensor integrated device is cultured and matured in the bioelectrode culture device.

2. The multifunctional bioelectrochemical contaminant detection device according to claim 1, wherein: and the upper part of the anode chamber of the multifunctional bioelectrochemistry pollutant detection device is also provided with a pH meter probe mounting hole site, a conductivity meter probe mounting hole site, a thermometer mounting hole site and a graphite electrode mounting hole site.

3. The multifunctional bioelectrochemical contaminant detection device according to claim 2, wherein: the anode chamber of the multifunctional bioelectrochemistry pollutant detection device is also provided with a water outlet and a water inlet, and the water outlet is positioned above the water inlet.

4. The multifunctional bioelectrochemical contaminant detection device according to claim 3, wherein: and a cathode chamber of the multifunctional bioelectrochemistry pollutant detection device is also provided with a liquid changing hole.

5. The multifunctional bioelectrochemical contaminant detection device according to any one of claims 1 to 4, wherein: the method for culturing and maturing the bioelectrode in the bioelectrode culture device comprises the following steps: step 1): operating the bioelectrode culture apparatus in MFC mode, adding an inoculation solution into the anode chamber to inoculate the bioelectrode, wherein the inoculation process lasts for five days, and the inoculation solution is replaced every day, wherein the inoculation solution is a mixture of 50% of inoculum and 50% of matrix solution, the matrix solution is 50mM phosphate buffer solution and contains 12.5mL L of L^-1Trace minerals, 5mL L^-1Vitamins, and 1.50gBOD L^-1Glucose; the inoculum is sewage of microorganisms with electricity generation function;

step 2): after the inoculation process is finished, the bioelectrode is cultured in a matrix solution in a sequential batch mode, and the matrix solution is changed every day until the bioelectrode grows mature;

wherein the cathode chamber solution is 50mM PBS solution containing 12.5mL L during the process of inoculating and culturing the bioelectrode^-1Trace minerals, 5mL L^-1Vitamins, changed every 7 days.

6. The multifunctional bioelectrochemical contaminant detection device according to claim 5, wherein: the sewage of the microorganisms with the electricity generating function in the step 1) refers to sewage containing key electricity generating microorganisms existing in natural environment or after artificial domestication, and comprises anoxic section sewage of an urban sewage treatment plant and slaughter house wastewater.

7. The multifunctional bioelectrochemical contaminant detection device according to claim 6, wherein: the electricity-producing microorganisms include microorganisms of the genus Geobacter.

8. The multifunctional bioelectrochemical contaminant detection device according to claim 1, wherein: the foamed nickel air electrode is a non-biological electrode or a counter electrode, the silver/silver chloride electrode is a reference electrode, and the carbon-based electrode is a biological electrode.

9. The multifunctional bioelectrochemical contaminant detection device according to claim 3, wherein: the water inlet and the water outlet are round holes with the diameter of 4mm formed in the two sides of the wall surface of the anode chamber.

Technical Field

The invention relates to a bioelectrochemical reaction, which is mainly applied to the measurement of the concentration of pollutants such as nitrite, BOD and the like in wastewater, in particular to a multifunctional bioelectrochemical pollutant detection device which can be used for the detection of BOD and nitrite in wastewater.

Background

With urban construction and industrial development, the yield of industrial sewage and urban sewage increases year by year. The treatment and discharge of sewage is one of the main tasks of the current environmental protection work. In order to evaluate the treated degree of the pollutants in the sewage and whether the treated sewage meets the discharge standard which can be accepted by the environment, the concentration of the pollutants in the sewage needs to be monitored.

The bioelectrochemical sensor is an efficient method for detecting pollutants in sewage, and the content of the pollutants in the sewage is quantified by utilizing an electric signal of a bioelectrochemical system. Compared with the traditional detection technology, the bioelectrochemical sensor has the advantages of shorter detection time, less chemical reagent investment, lower labor cost and the like. The bioelectrochemical sensor can realize selective test on different pollutants by utilizing different electroactive microorganisms. However, the bioelectrochemical sensor using microorganisms with different electrical activities (such as organic matter oxidation electroactive microorganisms and nitrite reduction electroactive microorganisms) as the detection unit generally needs to use different electrochemical system configurations including a bioanode electrochemical system and a biocathode electrochemical system. Therefore, the conventional bioelectrochemical sensor device can only be used for detecting a single pollutant, and cannot fully utilize mixed bacteria biological electrodes containing different electroactive microorganisms to synchronously detect different pollutants (such as BOD and nitrite).

The bioelectrochemical reaction process is influenced by the physical parameters of the sewage, including the temperature, conductivity, pH value and the like of the sewage. If the concentration of pollutants in sewage is monitored in real time, the bioelectrochemical sensor is required to generate an electric signal and correct the electric signal according to real-time sewage physical parameters. However, at present, a bioelectrochemical sensor device capable of simultaneously measuring a physical property parameter of wastewater and converting a contaminant concentration into an electric signal is lacking.

Disclosure of Invention

The invention aims to solve the technical problem of overcoming the defects of the prior art and provides a multifunctional bioelectrochemical pollutant detection device.

A multifunctional bioelectrochemical pollutant detection device comprises a reactor with a two-chamber structure, wherein the reactor comprises an anode chamber and a cathode chamber, the anode chamber and the cathode chamber are separated by a cation exchange membrane, a non-biological electrode is arranged in the cathode chamber, and a reference electrode and a biological electrode are arranged in the anode chamber;

when a biological electrode and a non-biological electrode in the multifunctional bioelectrochemical pollutant detection device are externally connected with a resistor to form a loop, the resistor and a reactor form a biological electrode culture device; or the like, or, alternatively,

when a biological electrode, a non-biological electrode and a reference electrode in the multifunctional bioelectrochemical pollutant detection device are connected with a potentiostat in a three-electrode system, the reactor and the potentiostat form a bioelectrochemical sensor integrated device;

wherein, the bioelectrode required by the bioelectrochemical sensor integrated device is cultured and matured in the bioelectrode culture device.

Preferably, the upper part of the anode chamber of the multifunctional bioelectrochemical pollutant detection device is also provided with a pH meter probe mounting hole site, a conductivity meter probe mounting hole site, a thermometer mounting hole site and a graphite electrode mounting hole site.

Preferably, the anode chamber of the multifunctional bioelectrochemistry pollutant detection device is further provided with a water outlet and a water inlet, and the water outlet is positioned above the water inlet.

Preferably, a liquid changing hole is further formed in a cathode chamber of the multifunctional bioelectrochemical pollutant detection device.

Preferably, the culturing and maturing of the bioelectrode in the bioelectrode culturing device comprises the following steps: step 1): operating the bioelectrode culture device in an MFC mode, adding an inoculation solution into the anode chamber to inoculate the bioelectrode, wherein the inoculation process lasts for five days, and the inoculation solution is replaced every day, wherein the inoculation solution is 50 percent of inoculationMixture of the substance and 50% matrix solution, which is 50mM phosphate buffer solution and contains 12.5mL L^-1Trace minerals, 5mL L^-1Vitamins, and 1.50gBOD L^-1Glucose; the inoculum is sewage of microorganisms with electricity generation function;

step 2): after the inoculation process is finished, the bioelectrode is cultured in a matrix solution in a sequential batch mode, and the matrix solution is changed every day until the bioelectrode grows mature;

wherein the cathode chamber solution is 50mM PBS solution containing 12.5mL L during the process of inoculating and culturing the bioelectrode^-1Trace minerals, 5mL L^-1Vitamins, changed every 7 days.

Preferably, the wastewater containing the electricity-generating functional microorganisms in the step 1) refers to wastewater containing key electricity-generating microorganisms existing in natural environment or after artificial domestication, and comprises anoxic section wastewater of an urban sewage treatment plant and slaughterhouse wastewater.

Preferably, the electricity-producing microorganisms include microorganisms of the genus Geobacter.

Preferably, the foamed nickel air electrode is a non-biological electrode or a counter electrode, the silver/silver chloride electrode is a reference electrode, and the carbon-based electrode is a biological electrode.

Preferably, the water inlet and the water outlet are round holes with the diameter of 4mm formed in two sides of the wall surface of the anode chamber.

The present application is further described below:

aiming at the problems, the invention uses a double-chamber bioreactor configuration, combines an electrochemical workstation (namely a constant potential rectifier), a thermometer, a pH meter and a conductivity meter, and jointly constructs a bioelectrochemical sensor device which can be used for testing different sewage pollutants and can be synchronously corrected based on sewage physical property parameters.

In order to solve the technical problem, the solution of the invention is as follows:

a bioelectrochemical sensor device which can be used for testing different sewage pollutants and can be synchronously corrected based on sewage physical property parameters. The device can be used for bioelectrode culture and can simultaneously obtain the response electric signals of the bioelectrochemical sensor to different sewage pollutants and the sewage physical parameters.

A double-chamber bioreactor configuration is adopted, a carbon-based material (comprising a carbon brush, a carbon cloth, a graphite sheet and the like) is used for enriching an electroactive microorganism culture biological electrode, a foam nickel air electrode is used as a non-biological electrode, and silver/silver chloride is used as a reference electrode. The reactor is preset with water inlet and outlet holes for replacing solution and holes for loading reference electrode, thermometer, pH meter probe and conductivity meter probe. And in the bioelectrode culture stage, sewage containing electrogenesis function microorganisms is used as an inoculum, and the reactor is externally connected with a resistor and runs in a closed circuit in an MFC mode. When the sewage pollutant is tested, the reactor is connected to an electrochemical workstation by a three-electrode system, the bioelectrode is controlled to operate under different potentials, different bioelectrochemical reactions are carried out, and the concentrations of different pollutants in the sewage can be tested. And correcting the electric signals according to the temperature, pH and conductivity of the sewage obtained by the thermometer, the pH meter and the conductivity meter, so as to realize the accurate detection of the concentration of the pollutants in the sewage.

In the invention, the configuration of the double-chamber bioreactor refers to a reactor which adopts a classical configuration and has a cubic two-chamber structure, wherein the anode chamber has the inner diameter of 35mm, the length of 40mm and the volume of 40mL, and the cathode chamber has the inner diameter of 35mm, the length of 20mm and the volume of 20 mL. The two chambers are separated by a cation exchange membrane.

According to the invention, the hole sites of the water inlet and the water outlet preset on the reactor mean that circular holes with the diameter of 4mm are arranged on two sides of the wall surface of the anode chamber.

The hole sites for loading the reference electrode, the thermometer, the pH meter probe and the conductivity meter probe in the invention refer to circular holes with the aperture of 10mm on the upper part of the anode chamber of the reactor.

The invention relates to inoculation of sewage containing electricity-generating functional microorganisms, which is sewage containing key electricity-generating microorganisms existing in natural environment or after artificial domestication and comprises anoxic section sewage of an urban sewage treatment plant, slaughterhouse wastewater and the like. The key electrogenic microorganisms include microorganisms of the genus Geobacter and the like.

The external resistor of the reactor is characterized in that a graphite sheet electrode and a foam nickel air electrode of the reactor are connected with an external resistor by using a copper wire as a lead to form a closed loop, wherein the bioelectrode is an MFC anode, and the foam nickel air electrode is an MFC cathode.

In the invention, the reactor is connected to the electrochemical workstation by a three-electrode system, namely, a biological electrode of the reactor is used as an anode, a foam nickel air electrode is used as a counter electrode, and Ag/AgCl is used as a reference electrode and is connected to the electrochemical workstation.

In the invention, the bioelectrode is controlled at different potentials, namely, a specific voltage is applied between a reference electrode with relatively stable potential and the bioelectrode through an electrochemical workstation, so that the potential of the bioelectrode is controlled.

The anode chamber/working electrode chamber of the reactor is provided with a sample solution water inlet and a sample solution water outlet which are respectively positioned at the left side and the right side of the reactor cavity and have the diameter of 4 mm. The height of the water inlet from the bottom of the reactor is 8mm, and the height of the water outlet from the bottom of the reactor is 40 mm.

The reactor is matched with a resistance box and an electrochemical workstation, so that the two functions of bioelectrode culture and a bioelectrochemical sensor can be rapidly switched.

The reactor is matched with a thermometer, a pH meter and a conductivity meter, and can synchronously acquire sewage physical parameters including temperature, pH and conductivity during detection.

Description of the inventive principles:

the invention provides a multifunctional bioelectrochemical pollutant detection device which can realize the integration of bioelectrode culture and pollutant detection functions based on a double-chamber bioreactor structure, and the device can synchronously obtain sewage physical property parameters for synchronously correcting detection results in the test process.

The reactor is a biological electrode culture device and a bioelectrochemical sensor integrated device which are constructed by a double-chamber bioreactor and an electrochemical workstation. The mutual conversion of the bioelectrode culture device and the bioelectrochemical sensor is realized through two operation modes of an external resistor and an external bioelectrochemical workstation. And applying voltage between the bioelectrode and the reference electrode by using an electrochemical workstation, and regulating and controlling the type of the bioelectrochemical reaction generated on the bioelectrode in real time by using the difference of reaction potentials of different bioelectrochemical processes (BOD oxidation and nitrite reduction).

When the reactor is used as a bioelectrochemical sensor, the electric response of the biosensor to the concentration of pollutants in sewage and the physical parameters (temperature, pH and conductivity) of the sewage can be synchronously obtained through the combined action of the electrochemical workstation, the thermometer, the pH meter and the conductivity meter.

Compared with the prior art, the invention has the following beneficial effects:

1. the multifunctional bioelectrochemical pollutant detection device disclosed by the invention is flexible in layout, can realize the switching of a bioelectrochemical system between a bioelectrode culture mode and a potential control operation mode, saves the cost and can be repeatedly used.

2. The multifunctional bioelectrochemical pollutant detection device can realize real-time monitoring of different sewage pollutant concentrations by the same device by adjusting the bioelectrode potential through the electrochemical workstation.

3. The bioelectrochemical sensor integrated device constructed by the multifunctional bioelectrochemical pollutant detection device can simultaneously acquire pollutant concentration response signals and sewage physical property parameters, realize real-time detection and real-time correction and improve the detection precision of the bioelectrochemical sensor.

Drawings

FIG. 1 is a schematic view of the multifunctional bioelectrochemical contaminant detection device according to the present invention (wherein, FIG. 1-1 is a side view of the multifunctional bioelectrochemical contaminant detection device according to the present invention, and FIG. 1-2 is a top view of the multifunctional bioelectrochemical contaminant detection device according to the present invention);

FIG. 2 is a circuit diagram of the multifunctional bioelectrochemical contaminant detection device of the present invention in different modes of operation (wherein FIG. 2-1 is a circuit diagram of the multifunctional bioelectrochemical contaminant detection device of the present invention in MFC mode of operation, and FIG. 2-2 is a circuit diagram of the multifunctional bioelectrochemical contaminant detection device of the present invention in detector mode of operation);

FIG. 3 is a voltage curve of the multifunctional bioelectrochemical contaminant detector according to the present invention during the operation of the inoculated culture bioelectrode in the MFC mode.

Reference numerals: 1 is reactor anode chamber (operating in MFC mode) or working electrode chamber (operating in detector mode) — abbreviated as "anode chamber"; 2 is the reactor cathode compartment (operating in MFC mode) or the counter electrode compartment (operating in detector mode) — shortly called "cathode compartment"; 3 is a foam nickel air electrode; 4 is a silver/silver chloride reference electrode; 5 is a carbon-based electrode; 6 is a reactor cover plate; 7 is a cation exchange membrane; 8 is a water inlet of the anode chamber/the working electrode chamber of the reactor, which is called as a water inlet for short; 9 is a water outlet of the reactor anode chamber/working electrode chamber, which is called the water outlet for short; 10 is a graphite electrode mounting hole site; 11 is a reference electrode mounting hole site; 12 is a pH meter probe mounting hole site; 13 installing hole sites on the probes of the conductivity meter; 14 is a thermometer mounting hole site; 15 is a liquid changing hole of a cathode chamber/a counter electrode chamber of the reactor, which is called the liquid changing hole for short; 16 is the connection mode when the reactor operates in MFC mode; 17 is the connection mode when the reactor is operated in the detector mode; 18 is the external resistance of the reactor, which is called the resistance for short; and 19 is a constant potential rectifier, wherein C, R and W respectively correspond to a corresponding electrode binding post, a reference electrode corresponds to a binding post and a working electrode binding post.

Detailed Description

The invention is further described with reference to the following figures and specific examples, which are intended to better illustrate the invention and not to limit it.

Example 1

The multifunctional bioelectrochemical pollutant detection device of the invention operates the bioelectrode for the culture detector in an MFC mode by using the sludge water of the anoxic section of the sewage plant as an inoculum:

first, we used a reactor of a cubic two-chamber structure of classical configuration, with an anode chamber 1 of 35mm in inside diameter, 40mm in length and 40mL in volume, and a cathode chamber 2 of 35mm in inside diameter, 20mm in length and 20mL in volume. The two chambers are separated by a cation exchange membrane 7. A non-biological electrode (adopting a foam nickel air electrode 3) is arranged in the cathode chamber 2, and a reference electrode (adopting a silver/silver chloride reference electrode 4) and a biological electrode (adopting a carbon-based electrode 5) are arranged in the anode chamber.

The bioelectrode, which is a carbon-based electrode 5, was inoculated with a mixture of 50% (v/v) anoxic zone sludge water and 50% (v/v) matrix solution. The base solution was 50mM phosphate buffer (PBS; 11.466g L)^-1Disodium hydrogen phosphate dodecahydrate, 2.75g L^-1Sodium dihydrogen phosphate dihydrate) containing 12.5mL of L^-1Trace minerals, 5mL L^-1Vitamins, and 1.50g L^-1And (3) glucose. The inoculation process is carried out in the anode chamber 1, the inoculation solution is injected from the water inlet 8 of the anode chamber, the inoculation process lasts for five days, and the mixed solution for inoculation (namely the inoculation solution) is replaced every day. After the inoculation process is finished, the bioelectrode 5 is cultured in the matrix solution in a sequential batch mode, and the matrix solution is changed every day. In the above process (including the inoculation and bioelectrode incubation processes), the solutions in the cathode chamber 2 (indicated by reference numeral 2 in FIG. 1) were each 50mM PBS containing 12.5mL L of the solution^-1Trace minerals, 5mL L^-1Vitamins, and the cathode solution is replaced every 7 days through the cathode chamber liquid replacement hole 15.

The anode chamber of the multifunctional bioelectrochemistry pollutant detection device is also provided with a water outlet 9, and the water outlet is positioned above the water inlet. All solutions (including seed, matrix, cathode) were flushed with pure nitrogen for 20 minutes before addition to remove dissolved oxygen from the water. The MFC reactor (i.e. the reactor in the multifunctional bioelectrochemical pollutant detection device of the present invention) is constructed as a bioelectrode culture device, which is operated in a closed loop in an MFC mode, and in this case, the reactor is called as an MFC reactor), and a bioelectrode 5 and a non-bioelectrode 3 are connected with a resistor 18, and the circuits are connected by a copper wire (shown by reference numeral 16 in FIG. 2), and the non-bioelectrode is a foamed nickel air electrode 3. The voltage of the MFC reactor was recorded by a digital data collection instrument (keygage model 34970 a). The whole process of the inoculation culture of the bioelectrode is carried out in a constant temperature environment of 30 ℃.

The voltage profile of the MFC reactor during the seed culture in this example is shown in fig. 3.

According to the voltage of the MFC, the culture condition of the bioelectrode for the detector can be judged. Typically, MFC has a stable and repeatable voltage profile for more than 3 cycles after inoculation, i.e., it is believed that the bioelectrode has grown to be relatively stable and can be used as a detector for bioelectrochemical sensors for the detection of contaminants. As shown in fig. 3, the bioelectrode satisfied a stable and repeatable cycle of the voltage curve more than 3 on day 5, and thus the culture process for culturing the bioelectrode for a detector was completed over 5 days. The culture process of using the biological electrode for the MFC culture detector usually needs 4-9 days, and the biological electrode can be cultured in advance because the performance of the biological electrode is kept relatively stable after the biological electrode is cultured to be mature, so that the mature biological electrode can be used when the pollutant detection requirement is met.

Example 2

The multifunctional bioelectrochemical pollutant detection device of the invention is used as a bioelectrochemical sensor to detect the sewage pollutants (taking the detection of the BOD concentration of the sewage as an example)

A silver/silver chloride reference electrode, a pH meter probe, a conductivity meter probe and a thermometer are respectively arranged on a reference electrode mounting hole site 11, a pH meter probe mounting hole site 12, a conductivity meter probe mounting hole site 13 and a thermometer mounting hole site 14 which are reserved on a reactor. The reactor is connected with a potentiostat 19 by a three-electrode system (as shown by a mark 17 in figure 2), a carbon-based electrode 5 (a biological electrode), a foam nickel air electrode 3 and a silver/silver chloride 4 electrode are respectively used as working electrodes, a counter electrode and a reference electrode, wherein the reference electrode is arranged at a reference electrode mounting hole position 11, thereby constructing a bioelectrochemical sensor integrated device for testing sewage pollutants.

The reactor of the invention controls the potential of the working electrode according to the testing mode of the bioelectrochemical BOD sensor, carries out specific electrochemical scanning analysis and obtains an electric response signal related to the BOD concentration of the sewage. During the test, the pH meter is simultaneously passed (_PThe H meter is arranged in figure 2_PAt the position of the H meter probe mounting hole 12), a conductivity meter (a conductivity meter 13 is mounted on the conductivity meter in figure 2)At the meter-installing hole site 13), and a thermometer (thermometer 14 is installed at the thermometer-installing hole site 14 in fig. 2) to acquire the pH, conductivity, temperature values of the sewage. And correcting the electric response signal according to the pH, the conductivity and the temperature of the sewage to obtain the BOD concentration of the sewage.

In this example, the bioelectrochemical sensor is shown in table 1 for testing electrical signals and physical parameters (including pH, conductivity, and temperature) of different types of wastewater:

table 1 test results of this detector device in testing BOD in different sewages

When the bioelectrochemical sensor integrated device is tested in different sewage, the electrochemical response signals corresponding to BOD in the sewage are obtained, and the pH, the conductivity and the temperature value of the sewage can be synchronously tested to correct the test result of the bioelectrochemical BOD sensor. The result shows that the relative detection error of the detector device for different sewage is 3.9-6.7%, and the detector device is in the leading position in the existing BOD detector. The result shows that the reactor device can well solve the defect that the detection signal of the bioelectrochemical sensor is easy to be changed by the physical parameters of the sewage, and the detector can conveniently and quickly detect the pollutants in actual waste water of different types.

Example 3

The bioelectrochemical sensor integrated device constructed by the invention is used for adjusting detection parameters and realizing the detection of different pollutants.

The bioelectrochemical sensor integrated device regulates and controls the potential change of the bioelectrode through different potentiostat programs, so that different electrochemical tests are performed on the bioelectrode, and the detection of different pollutants by the same device is realized.

Taking BOD and nitrite detection as an example, when the device is used for BOD detection, LSV scanning is carried out on the biological electrode in a detected solution through a potentiostat, the scanning range is controlled in a potential range allowing the biological electrode to generate bioelectrochemistry oxidation BOD reaction, and forward potential scanning is adopted in scanning, so that the bioelectrochemistry oxidation reaction is facilitated, and the current signal generated in the BOD oxidation process is facilitated to be accurately obtained. When the bioelectrochemical sensor integrated device is used for nitrite detection, the bioelectrode is subjected to LSV scanning in a detected solution through the potentiostat, the scanning range is controlled in a potential range which allows the bioelectrochemical reduction nitrite reaction of the bioelectrode to occur, negative potential scanning is adopted in the scanning process, the bioelectrochemical reduction reaction is favorably generated, and a current signal generated in the nitrite reduction process is favorably acquired.