阅读说明：本技术 一种茅莓分级多糖、制备方法和在抗抑郁中的应用 (Rubus parvifolius graded polysaccharide, preparation method and application in depression resistance ) 是由 于海涛 其他发明人请求不公开姓名 于 2020-08-17 设计创作，主要内容包括：本发明公开了一种茅莓分级多糖、制备方法和在抗抑郁中的应用。本发明提供了一种从茅梅叶中提取分离得到的醇溶性介于40％～50％之间的分级多糖,其中多糖含量为(95.3±2.7)％,纯度高；本发明发现该茅莓分级多糖在小鼠悬尾测试和小鼠强迫游泳测试中可以显著缩短悬尾不动时间和游泳不动时间,发挥了与阳性药类似的作用,具有明显的抗抑郁活性。本发明提供的茅莓分级多糖为一种天然活性多糖,无毒副作用。(The invention discloses rubus parvifolius graded polysaccharide, a preparation method and application thereof in depression resistance. The invention provides a graded polysaccharide with alcohol solubility between 40-50% extracted and separated from the leaves of the cymbopogon citratus, wherein the content of the polysaccharide is (95.3 +/-2.7)%, and the purity is high; the invention discovers that the rubus parvifolius graded polysaccharide can obviously shorten the tail suspension immobility time and the swimming immobility time in a mouse tail suspension test and a mouse forced swimming test, plays a similar role with a positive drug and has obvious antidepressant activity. The rubus parvifolius graded polysaccharide provided by the invention is a natural active polysaccharide and has no toxic or side effect.)

1. A rubus parvifolius graded polysaccharide is characterized in that: is a fractional polysaccharide with alcohol solubility between 40 percent and 50 percent which is extracted and separated from the cymbopogon citratus leaves.

2. A method for preparing the rubus parvifolius graded polysaccharide of claim 1, which comprises the following steps:

(1) defatting dried leaves of Rubus parvifolius;

(2) taking the degreased thamnis lanceolata leaves, adding distilled water, heating for extraction, filtering, collecting filtrate and filter residue, repeatedly extracting the filter residue once, combining the extracting solutions obtained by the two extractions, concentrating under reduced pressure, centrifuging to remove insoluble substances, adding four times of volume of ethanol, stirring uniformly, standing for precipitation, centrifuging, and collecting the precipitate; redissolving the precipitate with distilled water, dialyzing with 8000-14000 Da dialysis bag distilled water, collecting the liquid in the dialysis bag, concentrating and freeze-drying to obtain total polysaccharide;

(3) redissolving the total polysaccharide with distilled water, centrifuging to remove insoluble substances, adding equal volume of ethanol, stirring uniformly, standing for precipitation, centrifuging, and collecting the precipitate; redissolving the precipitate with distilled water, dialyzing with 8000-14000 Da dialysis bag distilled water, collecting the liquid in the dialysis bag, concentrating and freeze-drying to obtain crude polysaccharide;

(4) redissolving the crude polysaccharide by using distilled water, centrifuging to remove insoluble substances, adding ethanol according to the volume ratio of 7:3, uniformly stirring, standing for precipitation, centrifuging to collect supernatant, concentrating, and freeze-drying to obtain the graded polysaccharide.

3. The method of claim 2, wherein: soaking the degreased part in absolute ethyl alcohol in the step (1).

4. The method of claim 2, wherein: the temperature of the heating extraction in the step (1) is 85 ℃.

5. Use of the rubus parvifolius fractionated polysaccharide of claim 1 in the manufacture of an antidepressant medicament.

Technical Field

The invention relates to the field of polysaccharides, and in particular relates to rubus parvifolius graded polysaccharide, a preparation method and application thereof in depression resistance.

Background

Depression is an affective disorder disease, which is caused by genetic, endocrine, metabolic, neurobiological and environmental factors and mainly clinically shows symptoms such as low mood, lack of interest, anhedonia and the like. Depression has a high rate of recurrence and suicide, and the world health organization indicates depression or the second largest disease that will follow coronary heart disease. At present, the depression symptoms can be relieved by western medicine treatment to a certain extent, but the problems of high recurrence rate, large side effect and the like exist.

Natural active polysaccharides are natural macromolecules which are widely present in plants, animals and microorganisms and are composed of a plurality of monosaccharides through alpha-or beta-glycosidic bonds, and are widely used in health food and medical industries due to various biological activities and no toxic or side effects.

At present, no extraction and separation of rubus parvifolius polysaccharide and application in antidepressant treatment are seen.

Disclosure of Invention

The invention aims to overcome the defects and shortcomings in the prior art and provides rubus parvifolius graded polysaccharide, a preparation method and application in depression resistance.

The technical scheme for realizing the purpose is as follows:

a rubus parvifolius graded polysaccharide is a graded polysaccharide which is extracted and separated from the leaves of the rubus parvifolius and has the alcohol solubility of between 40 and 50 percent.

The preparation method of the rubus parvifolius graded polysaccharide comprises the following steps:

(1) defatting dried leaves of Rubus parvifolius;

(2) taking the degreased thamnis lanceolata leaves, adding distilled water, heating for extraction, filtering, collecting filtrate and filter residue, repeatedly extracting the filter residue once, combining the extracting solutions obtained by the two extractions, concentrating under reduced pressure, centrifuging to remove insoluble substances, adding four times of volume of ethanol, stirring uniformly, standing for precipitation, centrifuging, and collecting the precipitate; redissolving the precipitate with distilled water, dialyzing with 8000-14000 Da dialysis bag distilled water, collecting the liquid in the dialysis bag, concentrating and freeze-drying to obtain total polysaccharide;

(3) redissolving the total polysaccharide with distilled water, centrifuging to remove insoluble substances, adding equal volume of ethanol, stirring uniformly, standing for precipitation, centrifuging, and collecting the precipitate; redissolving the precipitate with distilled water, dialyzing with 8000-14000 Da dialysis bag distilled water, collecting the liquid in the dialysis bag, concentrating and freeze-drying to obtain crude polysaccharide;

(4) redissolving the crude polysaccharide by using distilled water, centrifuging to remove insoluble substances, adding ethanol according to the volume ratio of 7:3, uniformly stirring, standing for precipitation, centrifuging to collect supernatant, concentrating, and freeze-drying to obtain the graded polysaccharide.

Preferably, the step (1) is carried out by soaking in absolute ethyl alcohol for degreasing.

Preferably, the temperature of the heated extraction in step (1) is 85 ℃.

The application of the rubus parvifolius graded polysaccharide in the preparation of antidepressant drugs.

Has the advantages that:

1. the invention provides a graded polysaccharide with alcohol solubility between 40-50% extracted and separated from the leaves of the cymbopogon citratus, wherein the content of the polysaccharide is (95.3 +/-2.7)%, and the purity is high.

2. The invention discovers that the rubus parvifolius graded polysaccharide can obviously shorten the tail suspension immobility time and the swimming immobility time in a mouse tail suspension test and a mouse forced swimming test, plays a similar role with a positive drug and has obvious antidepressant activity.

3. The rubus parvifolius graded polysaccharide provided by the invention is a natural active polysaccharide and has no toxic or side effect.

Drawings

FIG. 1 is an SEM image (500X) of the fractionated polysaccharide of rubus parvifolius;

FIG. 2 is a FT-IR plot of the fractionated polysaccharide of rubus parvifolius;

FIG. 3 shows the results of the tail suspension test of mice;

figure 4 shows the results of the mouse swimming test.

Detailed Description