阅读说明：本技术 青稞β-葡聚糖的提取方法 (Method for extracting highland barley beta-glucan ) 是由 王福清 于 2020-08-03 设计创作，主要内容包括：本发明公开了青稞β-葡聚糖的提取方法,包括：将10重量份的青稞麸皮粉加入60重量份的水中,搅匀,加入1.5重量份的四氧化三铁纳米颗粒水分散液,搅匀；将所得混合液预冷至0℃,转入-10℃冷冻12h,冷冻过程中,向混合液中间歇性施加600mT的交变磁场；将所得冷冻品于解冻,加热至45℃,保温搅拌提取2h,离心,得第一上清液和第一沉淀；向第一沉淀中加入60重量份的pH为7.5的氢氧化钠溶液,混匀,加热至45℃,保温搅拌提取1h,离心,得第二上清液和第二沉淀；合并第一上清液和第二上清液,调节pH至7.0,经浓缩、醇沉、离心、干燥处理,得青稞β-葡聚糖。本发明的方法可显著提高β-葡聚糖的提取率和纯度。(The invention discloses a method for extracting highland barley beta-glucan, which comprises the following steps: adding 10 parts by weight of highland barley bran powder into 60 parts by weight of water, stirring uniformly, adding 1.5 parts by weight of ferroferric oxide nanoparticle water dispersion, and stirring uniformly; pre-cooling the obtained mixed solution to 0 ℃, transferring to-10 ℃ for freezing for 12h, and applying an alternating magnetic field of 600mT to the mixed solution intermittently in the freezing process; thawing the obtained frozen product, heating to 45 ℃, preserving heat, stirring, extracting for 2 hours, and centrifuging to obtain a first supernatant and a first precipitate; adding 60 parts by weight of sodium hydroxide solution with the pH value of 7.5 into the first precipitate, uniformly mixing, heating to 45 ℃, preserving heat, stirring, extracting for 1 hour, and centrifuging to obtain a second supernatant and a second precipitate; and (3) combining the first supernatant and the second supernatant, adjusting the pH to 7.0, and performing concentration, alcohol precipitation, centrifugation and drying treatment to obtain the highland barley beta-glucan. The method can obviously improve the extraction rate and purity of the beta-glucan.)

1. The method for extracting highland barley beta-glucan is characterized by comprising the following steps:

step one, adding 10 parts by weight of highland barley bran powder into 60 parts by weight of water, uniformly stirring, adding 1.5 parts by weight of ferroferric oxide nanoparticle water dispersion, and uniformly stirring again to obtain a first mixed solution;

secondly, precooling the first mixed solution to 0 ℃, then transferring the first mixed solution to-10 ℃ for freezing treatment for 12 hours, and in the freezing process, intermittently applying an alternating magnetic field of 600mT to the first mixed solution, wherein the magnetic treatment time is 15min each time and the interval time is 30min, so as to obtain a frozen product;

naturally thawing the frozen product at room temperature to obtain a second mixed solution;

step four, heating the second mixed solution to 45 ℃, preserving heat, stirring and extracting for 2 hours, taking out, and centrifuging to obtain a first supernatant and a first precipitate;

step five, adding 60 parts by weight of sodium hydroxide solution with the pH value of 7.5 into the first precipitate, uniformly mixing, heating to 45 ℃, preserving heat, stirring, extracting for 1 hour, taking out, and centrifuging to obtain a second supernatant and a second precipitate;

step six, combining the first supernatant and the second supernatant, adjusting the pH to 7.0, and concentrating under reduced pressure to 1/8 of the original volume to obtain a concentrated solution;

and seventhly, adding 40 parts by weight of absolute ethyl alcohol into the concentrated solution, uniformly stirring, carrying out alcohol precipitation treatment at 4 ℃ for 12 hours, taking out, centrifuging, taking out the precipitate, and drying to obtain the highland barley beta-glucan.

2. The highland barley beta-glucan extraction method according to claim 1, wherein the concentration of the ferroferric oxide nanoparticle aqueous dispersion is 5mg/ml, and the particle size of the ferroferric oxide nanoparticles is 20 nm.

3. The method for extracting highland barley beta-glucan according to claim 1, wherein in the second step, the magnetic treatment is started when the central temperature of the first mixed solution reaches-3 ℃ in the freezing process.

4. The method for extracting highland barley beta-glucan as claimed in claim 1, wherein the alkali extraction process in the fifth step is performed in a self-made extraction tank, the self-made extraction tank comprises a tank body which is a sandwich tank, the upper part of the outer wall of the tank body is provided with a liquid inlet, and the lower part of the outer wall of the tank body is provided with a liquid outlet; the tank cover is arranged at the top of the tank body and can seal the tank body, and the tank cover is detachably connected with the tank body; stirring portion, it is located the jar is internal and with the coaxial setting of jar body, stirring portion includes the (mixing) shaft, and its vertical locating the middle part of the jar body, the (mixing) shaft is by locating motor drive on the cover rotates, two bar magnet, its edge the direction of height interval of (mixing) shaft sets up and lies in same vertical plane, arbitrary bar magnet with the (mixing) shaft is perpendicular and can dismantle the connection, just the magnetic pole of two bar magnet's adjacent end is different.

5. The method for extracting highland barley beta-glucan according to claim 4, wherein the stirring part further comprises a first stirring blade which is arranged between the two bar magnets, the first stirring blade is spirally arranged along the height direction of the stirring shaft and is fixedly connected with the stirring shaft; a plurality of second stirring leaves, it locates between two bar magnet, a plurality of second stirring leaves are followed the direction of height interval of (mixing) shaft is located the both sides of (mixing) shaft, arbitrary second stirring leaf with the (mixing) shaft is perpendicular and fixed connection, the last array of second stirring leaf is provided with a plurality of through-holes, wherein, the second stirring leaf with first stirring leaf contactless, first stirring leaf with second stirring leaf is made by ABS resin.

6. The highland barley beta-glucan extraction method according to claim 4, wherein the tank body comprises an inner tank body and an outer tank body sleeved outside the inner tank body, an interlayer is formed between the outer tank body and the inner tank body, the liquid inlet and the liquid outlet are respectively communicated with the interlayer, a flange is arranged on the top of the inner tank body, a bearing platform is arranged on the top of the outer tank body, a groove connected with the flange is arranged on the inner side of the bearing platform, a screw hole in threaded connection with the edge of the tank cover is arranged on the outer side of the bearing platform, and the inner tank body and the stirring shaft are both made of ABS resin.

Technical Field

The invention relates to the field of beta-glucan extraction. More specifically, the invention relates to a method for extracting highland barley beta-glucan.

Background

The highland barley is used as the main grain crop of Tibet, has rich beta-glucan content, the average content of 5.25 percent and the highest content of 8.62 percent, and is the most important grain crop. The highland barley beta-glucan has the effects of resisting tumors, regulating blood fat and blood sugar, improving immunity and the like, and has high health-care value. At present, the method for extracting highland barley beta-glucan mainly comprises water and alkali extraction, water and ethanol extraction, enzyme-method auxiliary extraction, high-pressure microwave auxiliary extraction, ultrasonic auxiliary extraction and the like, wherein the water and alkali extraction is mainly used, and compared with the traditional water extraction, although the water and alkali extraction can improve the extraction rate of the beta-glucan, the protein and starch in the extracting solution are seriously polluted, and the purity of the product is low.

Disclosure of Invention

The invention aims to provide an extraction method of highland barley beta-glucan, which can obviously improve the extraction rate and purity of the beta-glucan.

To achieve the objects and other advantages in accordance with the present invention, there is provided a highland barley beta-glucan extraction method, comprising the steps of:

step one, adding 10 parts by weight of highland barley bran powder into 60 parts by weight of water, uniformly stirring, adding 1.5 parts by weight of ferroferric oxide nanoparticle water dispersion, and uniformly stirring again to obtain a first mixed solution;

secondly, precooling the first mixed solution to 0 ℃, then transferring the first mixed solution to-10 ℃ for freezing treatment for 12 hours, and in the freezing process, intermittently applying an alternating magnetic field of 600mT to the first mixed solution, wherein the magnetic treatment time is 15min each time and the interval time is 30min, so as to obtain a frozen product;

naturally thawing the frozen product at room temperature to obtain a second mixed solution;

step four, heating the second mixed solution to 45 ℃, preserving heat, stirring and extracting for 2 hours, taking out, and centrifuging to obtain a first supernatant and a first precipitate;

step five, adding 60 parts by weight of sodium hydroxide solution with the pH value of 7.5 into the first precipitate, uniformly mixing, heating to 45 ℃, preserving heat, stirring, extracting for 1 hour, taking out, and centrifuging to obtain a second supernatant and a second precipitate;

step six, combining the first supernatant and the second supernatant, adjusting the pH to 7.0, and concentrating under reduced pressure to 1/8 of the original volume to obtain a concentrated solution;

and seventhly, adding 40 parts by weight of absolute ethyl alcohol into the concentrated solution, uniformly stirring, carrying out alcohol precipitation treatment at 4 ℃ for 12 hours, taking out, centrifuging, taking out the precipitate, and drying to obtain the highland barley beta-glucan.

Preferably, in the method for extracting highland barley beta-glucan, the concentration of the ferroferric oxide nano particle aqueous dispersion is 5mg/ml, and the particle size of the ferroferric oxide nano particles is 20 nm.

Preferably, in the step two, the magnetic treatment is started when the central temperature of the first mixed solution reaches-3 ℃ in the freezing process.

Preferably, in the method for extracting highland barley beta-glucan, the alkali extraction process in the fifth step is carried out in a self-made extraction tank, the self-made extraction tank comprises a tank body which is an interlayer tank, the upper part of the outer wall of the tank body is provided with a liquid inlet, and the lower part of the outer wall of the tank body is provided with a liquid outlet; the tank cover is arranged at the top of the tank body and can seal the tank body, and the tank cover is detachably connected with the tank body; stirring portion, it is located the jar is internal and with the coaxial setting of jar body, stirring portion includes the (mixing) shaft, and its vertical locating the middle part of the jar body, the (mixing) shaft is by locating motor drive on the cover rotates, two bar magnet, its edge the direction of height interval of (mixing) shaft sets up and lies in same vertical plane, arbitrary bar magnet with the (mixing) shaft is perpendicular and can dismantle the connection, just the magnetic pole of two bar magnet's adjacent end is different.

Preferably, in the method for extracting highland barley beta-glucan, the stirring part further comprises a first stirring blade arranged between the two bar magnets, and the first stirring blade is spirally arranged along the height direction of the stirring shaft and is fixedly connected with the stirring shaft; a plurality of second stirring leaves, it locates between two bar magnet, a plurality of second stirring leaves are followed the direction of height interval of (mixing) shaft is located the both sides of (mixing) shaft, arbitrary second stirring leaf with the (mixing) shaft is perpendicular and fixed connection, the last array of second stirring leaf is provided with a plurality of through-holes, wherein, the second stirring leaf with first stirring leaf contactless, first stirring leaf with second stirring leaf is made by ABS resin.

Preferably, the highland barley beta-glucan extraction method comprises the steps that the tank body comprises an inner tank body and an outer tank body sleeved outside the inner tank body, an interlayer is formed between the outer tank body and the inner tank body, the liquid inlet and the liquid outlet are respectively communicated with the interlayer, a flange is arranged at the top of the inner tank body, a bearing platform is arranged at the top of the outer tank body, a groove connected with the flange is arranged on the inner side of the bearing platform, a screw hole in threaded connection with the edge of the tank cover is arranged on the outer side of the bearing platform, and the inner tank body and the stirring shaft are both made of ABS resin.

The invention at least comprises the following beneficial effects:

firstly, adding a certain amount of ferroferric oxide nanoparticle water dispersion liquid into highland barley bran powder dispersion liquid in advance, transferring the obtained mixed liquid into-10 ℃ for slow freezing for 12 hours, intermittently applying an alternating magnetic field of 600mT to the mixed liquid in the slow freezing process, generating heat by the ferroferric oxide nanoparticles under the action of the alternating magnetic field, raising the surrounding temperature, dissolving/partially dissolving formed ice crystals, and then recrystallizing after the alternating magnetic field disappears to fully destroy the cell structure of the highland barley bran, promote the dissolution of beta-glucan and improve the extraction rate of the beta-glucan.

Secondly, two bar magnets which are positioned in the same vertical plane and are vertical to the stirring shaft are arranged on the stirring shaft, and the magnetic poles of the adjacent ends of the two bar magnets are different, so that in the alkali extraction and stirring process, on one hand, the solution can be magnetized, water molecules can more easily enter highland barley bran cells, the solubility of beta-glucan is increased, the extraction rate of the beta-glucan is further improved, and on the other hand, ferroferric oxide nanoparticles in the solution can be recovered.

And thirdly, a spiral first stirring blade and a second stirring blade vertical to the stirring shaft are arranged on the stirring shaft between the two strip magnets, and a through hole is formed in the second stirring blade to control the flowing direction of the solution in the stirring process, so that the solution moves along the circumferential direction of the stirring tank to the maximum extent, and the magnetization effect is improved.

Fourthly, compared with the existing water and alkali extraction, the extraction temperature and pH of the method are lower, the dissolution of starch and protein is reduced, and the purity of the product is higher.

Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.

Drawings

Fig. 1 is a schematic structural view of a homemade extraction tank according to one embodiment of the present invention.

Detailed Description

The present invention will be described in further detail with reference to the following examples and the accompanying drawings so that those skilled in the art can practice the invention with reference to the description.

It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.

It is to be noted that the experimental methods described in the following embodiments are all conventional methods unless otherwise specified, and the reagents and materials, if not otherwise specified, are commercially available; in the description of the present invention, the terms "lateral", "longitudinal", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.